Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Molecules ; 26(16)2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34443607

RESUMO

Cellular conformation of reduced pyridine nucleotides NADH and NADPH sensed using autofluorescence spectroscopy is presented as a real-time metabolic indicator under pressurized conditions. The approach provides information on the role of pressure in energy metabolism and antioxidant defense with applications in agriculture and food technologies. Here, we use spectral phasor analysis on UV-excited autofluorescence from Saccharomyces cerevisiae (baker's yeast) to assess the involvement of one or multiple NADH- or NADPH-linked pathways based on the presence of two-component spectral behavior during a metabolic response. To demonstrate metabolic monitoring under pressure, we first present the autofluorescence response to cyanide (a respiratory inhibitor) at 32 MPa. Although ambient and high-pressure responses remain similar, pressure itself also induces a response that is consistent with a change in cellular redox state and ROS production. Next, as an example of an autofluorescence response altered by pressurization, we investigate the response to ethanol at ambient, 12 MPa, and 30 MPa pressure. Ethanol (another respiratory inhibitor) and cyanide induce similar responses at ambient pressure. The onset of non-two-component spectral behavior upon pressurization suggests a change in the mechanism of ethanol action. Overall, results point to new avenues of investigation in piezophysiology by providing a way of visualizing metabolism and mitochondrial function under pressurized conditions.


Assuntos
NADP/química , NADP/metabolismo , NAD/química , NAD/metabolismo , Pressão , Fluorescência , Conformação Molecular
2.
Membranes (Basel) ; 11(7)2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34357166

RESUMO

Electrically conductive, highly flexible graphene membranes hold great promise for harvesting energy from ambient vibrations. For this study, we built numerous three-dimensional graphene ripples, with each featuring a different amount of compression, and performed molecular dynamics simulations at elevated temperatures. These ripples have a convex cosine shape, then spontaneously invert their curvature to concave. The average time between inversion events increases with compression. We use this to determine how the energy barrier height depends on strain. A typical convex-to-concave curvature inversion process begins when the ripple's maximum shifts sideways from the normal central position toward the fixed outer edge. The ripple's maximum does not simply move downward toward its concave position. When the ripple's maximum moves toward the outer edge, the opposite side of the ripple is pulled inward and downward, and it passes through the fixed outer edge first. The ripple's maximum then quickly flips to the opposite side via snap-through buckling. This trajectory, along with local bond flexing, significantly lowers the energy barrier for inversion. The large-scale coherent movement of ripple atoms during curvature inversion is unique to two-dimensional materials. We demonstrate how this motion can induce an electrical current in a nearby circuit.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...