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Sci Rep ; 12(1): 8623, 2022 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-35597874


Blind-structured illumination microscopy (blind-SIM) enhances the optical resolution without the requirement of nonlinear effects or pre-defined illumination patterns. It is thus advantageous in experimental conditions where toxicity or biological fluctuations are an issue. In this work, we introduce a custom convolutional neural network architecture for blind-SIM: BS-CNN. We show that BS-CNN outperforms other blind-SIM deconvolution algorithms providing a resolution improvement of 2.17 together with a very high Fidelity (artifacts reduction). Furthermore, BS-CNN proves to be robust in cross-database variability: it is trained on synthetically augmented open-source data and evaluated on experiments. This approach paves the way to the employment of CNN-based deconvolution in all scenarios in which a statistical model for the illumination is available while the specific realizations are unknown or noisy.

Aprendizado Profundo , Iluminação , Algoritmos , Artefatos , Microscopia de Fluorescência
Opt Express ; 28(24): 35427-35437, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33379657


Optical interrogation of tissues is broadly considered in biomedical applications. Nevertheless, light scattering by tissue limits the resolution and accuracy achieved when investigating sub-surface tissue features. Light carrying optical angular momentum or complex polarization profiles, offers different propagation characteristics through scattering media compared to light with unstructured beam profiles. Here we discuss the behaviour of structured light scattered by tissue-mimicking phantoms. We study the spatial and the polarization profile of the scattered modes as a function of a range of optical parameters of the phantoms, with varying scattering and absorption coefficients and of different lengths. These results show the non-trivial trade-off between the advantages of structured light profiles and mode broadening, stimulating further investigations in this direction.

Microscopia de Polarização/métodos , Imagens de Fantasmas , Espalhamento de Radiação , Biomimética , Luz , Modelos Biológicos
Phys Rev Lett ; 124(16): 160401, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32383956


Structured light is attracting significant attention for its diverse applications in both classical and quantum optics. The so-called vector vortex beams display peculiar properties in both contexts due to the nontrivial correlations between optical polarization and orbital angular momentum. Here we demonstrate a new, flexible experimental approach to the classification of vortex vector beams. We first describe a platform for generating arbitrary complex vector vortex beams inspired to photonic quantum walks. We then exploit recent machine learning methods-namely, convolutional neural networks and principal component analysis-to recognize and classify specific polarization patterns. Our study demonstrates the significant advantages resulting from the use of machine learning-based protocols for the construction and characterization of high-dimensional resources for quantum protocols.

Phys Rev Lett ; 122(2): 020503, 2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30720314


The capability to generate and manipulate quantum states in high-dimensional Hilbert spaces is a crucial step for the development of quantum technologies, from quantum communication to quantum computation. One-dimensional quantum walk dynamics represents a valid tool in the task of engineering arbitrary quantum states. Here we affirm such potential in a linear-optics platform that realizes discrete-time quantum walks in the orbital angular momentum degree of freedom of photons. Different classes of relevant qudit states in a six-dimensional space are prepared and measured, confirming the feasibility of the protocol. Our results represent a further investigation of quantum walk dynamics in photonics platforms, paving the way for the use of such a quantum state-engineering toolbox for a large range of applications.