Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 40
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(11): 1147-1151, 2019 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-31683403

RESUMO

Objective: To examine the association of pre-pregnancy body mass and weight gain during pregnancy with macrosomia. Methods: From January 2015 to December 2015, a total of 20 477 pregnant women were recruited by probabilistic proportional scale sampling with simple randomization in Sichuan, Yunnan and Guizhou Provinces. Basic information of pregnant women, weight gain during pregnancy and weight of newborn were collected. A multiple logistic regression model was used to assess the association between the pre-pregnancy body mass and gestational weight gain indicators with macrosomia. Results: 20 321 mother-infant were included in the final analysis. 20 321 pregnant women were (30.09±4.10) years old and delivered at (39.20±1.29) weeks, among which 12 341 (60.73%) cases were cesarean delivery. The birth weight of 20 321 infants were (3 292.26±431.67) grams, and 970 (4.77%) were macrosomia. The multiple logistic regression model showed that after adjusting for the age of women, compared to the normal weight group in the pre-pregnancy, the overweight and obesity group elevated the risk of macrosomia, with OR (95%CI) about 1.99 (95%CI: 1.69-2.35) and 4.05 (95%CI: 3.05-5.39), respectively. After adjusting for the age, the pre-pregnancy BMI, delivery weeks, delivery mode and infant's gender, compared to the weight-gain appropriate group, higher weight gain rate in the mid-pregnancy and excessive total gestational weight gain elevated the risk of macrosomia, with OR (95%CI) about 1.99 (95%CI: 1.66-2.39) and 1.80 (95%CI: 1.55-2.08), respectively. Conclusion: The overweight before pregnancy, obesity before pregnancy, the rate of weight gain in the second trimester and the high total weight gain during pregnancy could increase the risk of macrosomia.


Assuntos
Índice de Massa Corporal , Macrossomia Fetal/epidemiologia , Sobrepeso/epidemiologia , Ganho de Peso , Adulto , Peso ao Nascer , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Recém-Nascido , Obesidade/epidemiologia , Gravidez , Complicações na Gravidez/epidemiologia , Resultado da Gravidez , Segundo Trimestre da Gravidez , Fatores de Risco
2.
Zhonghua Yi Xue Za Zhi ; 98(46): 3737-3740, 2018 Dec 11.
Artigo em Chinês | MEDLINE | ID: mdl-30541213

RESUMO

Objective: To discuss the efficacy and safety of subinguinal microsurgical varicocelectomy in the treatment of non-obstructive azoospermia (NOA) with varicocele. Methods: The clinical data of 141 patients with NOA and varicocele who underwent subinguinal microsurgical varicocelectomy from March 2015 to June 2017 in Shanghai General Hospital was collected.One hundred and ten patients suffered from varicocele on the left side, 1 on the right side, and the rest (30 cases) were bilateral varicocele. Grade Ⅰ varicocele were found on 7 sides (the right and left side was count respectively), grade Ⅱ on 121 sides, and grade Ⅲ on 43 sides. Sperm analysis, pregnancy rate and complications were recorded after at least 6 months since operation. Results: Eleven cases were lost during the follow-up. Eighteen of the remaining 130 NOA patients processed successful sperm retrieval in post-operative semen analysis (18/130, 13.8%). Six couples(6/130, 4.6%) succeeded in natural pregnancy. Five couples (5/130, 3.8%)underwent successful pregnancy following with intracytoplasmic sperm injection(ICSI). Twenty-six out of the remaining 112 patients underwent the micro dissection testicular sperm extraction (micro-TESE), and 4 patients got a successful sperm retrieval (4/26, 15.4%). Among them, 2 couples had successful pregnancy with ICSI. Totally 2 cases of postoperative infection of incision were found. Conclusions: Microsurgical varicocelectomy had a beneficial effect on sperm quality of patients suffered from NOA with varicocele to some extent, even leading to unassisted pregnancy or avoiding micro-TESE before ICSI. Microsurgical varicocelectomy could be applied in the treatment of NOA with varicocele.


Assuntos
Azoospermia , Varicocele , Azoospermia/cirurgia , China , Feminino , Humanos , Masculino , Gravidez , Estudos Retrospectivos , Injeções de Esperma Intracitoplásmicas , Recuperação Espermática
3.
Zhonghua Liu Xing Bing Xue Za Zhi ; 39(10): 1319-1323, 2018 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-30453430

RESUMO

Objective: To explore the effects of both pre-gestational BMI and gestational weight gain (GWG) on the birth weight of neonates. Methods: A total of 5 395 pregnant women were selected from the Southwest areas of China (Sichuan/Yunnan/Guizhou) and were divided into groups as pre-gestational underweight, normal weight, overweight and obesity, according to the WHO Recommendation on BMI Classification. Guidelines on Pregnancy weight were adopted from the Institute of Medicine to confirm the accuracy of GWG. Multinomial logistic regression model was used to assess the associations between pregestational BMI and GWG, on the birth weight of the neonates. Results: After adjusting for related confounders, low pre-gestational BMI appeared as a risk factor for SGA (OR=1.91, 95%CI: 1.47-2.50), and was also associated with the decreased risk of LGA (OR=0.55, 95%CI: 0.47-0.66). Inadequate GWG was both associated with the increased risk of delivering SGA (OR=1.57, 95%CI: 1.21-2.03) and the decreased risk of LGA (OR=0.48, 95%CI: 0.41-0.57). Pre-gestational overweight/obesity (OR=1.85, 95%CI: 1.58-2.17) and excessive GWG (OR=1.87, 95%CI: 1.67- 2.11) were both positively associated with the risks on LGA. Data from the stratified analysis indicated that inadequate GWG was positively associated with the risk of SGA among underweight or normal weight women (all P<0.05), but not with those overweight/obese women. Conclusions: Pre-gestational BMI and GWG were important influencing factors on the birth weight of neonates. Health education programs for pregnant women should be intensified and gestational weight gain should also be reasonably under control.


Assuntos
Peso ao Nascer , Índice de Massa Corporal , Ganho de Peso na Gestação , Ganho de Peso , Adulto , China , Feminino , Humanos , Recém-Nascido , Obesidade , Sobrepeso , Gravidez , Resultado da Gravidez , Estudos Prospectivos
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 52(6): 668-672, 2018 Jun 06.
Artigo em Chinês | MEDLINE | ID: mdl-29886691

RESUMO

Objective: To evaluate the effectiveness and to explore the releated factors of antiretroviral therapy among HIV/AIDS patients in Liangshan Yi Autonomous Prefecture, Sichuan Province. Methods: The method of convenience sampling was adopted in July 2017 to select the research objects who were accepted antiretroviral therapy (ART) over 6 months, older than 18 years and had HIV viral load in 2016, totally 400 cases. A retrospective study was used to collect the data, including social demography, medicine use, information of medical service acquisition, their own behaviors and cognition. 395 questionnaires were effectively recovered. χ(2) test and logistic regression were performed to examine relationships between factors and effects. Results: All of the 395 respondents were Yi-nationality. The average age of all cases was (39.23±7.52) years old and 223 were male (56.5%). Among 395 cases patients who were detect Viral load in 2016, 221 cases were under the number of 400 copies, thze effective rate of ART was 55.9%. Multivariate analysis showed that HIV/AIDS patients who missed the medication during the antiviral therapy had poor antiviral effects. Compared to those who adhered to medication, the treatment-ineffective OR (95%CI) of the patients missing the medication during the therapy was 7.06 (3.67-13.58); Compared to those who had adverse reactions that affect the therapy, the treatment-ineffective OR (95%CI) of the patients with mild adverse reactions that did not affect the therapy was 0.45 (0.23-0.87); Compared to the patients who used drugs during the treatment, the treatment-ineffective OR (95%CI) value of the antiretroviral therapy effect of non-drug users was 0.39 (0.16-0.91);Compared to the patients who have a correct cognition that insisting on taking medicine correctly can extend their life expectancy as a common person, the treatment-ineffective OR (95%CI) values for those who hold the view that could be prolonged by 10-20 years and not/unknown were 4.18 (1.59-10.99) and 6.64 (2.67-16.53). Conclusion: The HIV/AIDS patients who receive ART were less effective in Liangshan, Prefecture. Missings drugs is one of the main influencing factors for the ineffective treatment.


Assuntos
Antirretrovirais/uso terapêutico , Infecções por HIV/tratamento farmacológico , Adulto , China , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Resultado do Tratamento , Carga Viral
5.
Zhonghua Fu Chan Ke Za Zhi ; 52(10): 657-661, 2017 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-29060962

RESUMO

Objective: To analyze the clinical characteristics and perinatal outcomes of listeriosis during pregnancy. Methods: From July 2010 to April 2017, 70 131 women delivered in West China Second University Hospital. Nineteen cases were confirmed as listeriosis. The clinical symptoms, laboratory results, pathogens, placenta pathology and perinatal outcomes were analyzed retrospectively. Results: The median age of the 19 cases was 29.7 (19.0-42.0) years old. The median time before diagnosis was 4.8(0.5-19.0) days. The main clinical symptoms at first visits were high fever (17/19), increased white blood cells (18/19), abdominal pain (12/19). Listeria was found in samples of mother's blood (11/19), vaginal secretions (15/19), placenta (1/19), neonatal blood (4/19), neonatal phlegm (5/19) and neonatal ear secretions (1/19), respectively. Inflammation of placenta was identified in all 19 cases. Among the 19 cases, 1 was grade Ⅰ chorioamnionitis, 4 was grade Ⅱ, 5 was grade Ⅲ and 9 was grade Ⅵ. Only 4 newborn survived after therapy, and others suffered perinatal death, including 8 cases of intrauterine death, 3 cases of miscarriage and 6 cases of treatment failure. Conclusions: Listeriosis has characteristics of acute onset, quick development and high morbidity during pregnancy. The empiric use of antibiotics might not cover listeria. The understanding of listeriosis should be improved.


Assuntos
Morte Fetal/etiologia , Listeria monocytogenes/isolamento & purificação , Listeriose/diagnóstico , Complicações Infecciosas na Gravidez/diagnóstico , Aborto Espontâneo , Adulto , Antibacterianos/uso terapêutico , China/epidemiologia , Corioamnionite/tratamento farmacológico , Corioamnionite/microbiologia , Feminino , Humanos , Recém-Nascido , Listeriose/sangue , Listeriose/tratamento farmacológico , Listeriose/epidemiologia , Parto , Placenta , Gravidez , Complicações Infecciosas na Gravidez/sangue , Complicações Infecciosas na Gravidez/tratamento farmacológico , Complicações Infecciosas na Gravidez/epidemiologia , Resultado da Gravidez , Estudos Retrospectivos
6.
Zhonghua Liu Xing Bing Xue Za Zhi ; 38(8): 1102-1106, 2017 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-28847063

RESUMO

Objective: To investigate the epidemiologic and behavioral characteristics of HIV among community population in Liangshan prefecture. Methods: We collected social demographic, behavioral and serological information by means of the monitoring questionnaire and serological tests. Data was analyzed by using the chi-square test and logistic regression. Results: From April to June of 2010 to 2015, 14 092 cases of community population were selected as the study objects, with 267 cases diagnosed as HIV positive patients. The HIV positive rates were 3.24%, 3.07%, 1.17%, 1.38%,1.42% and 1.25%, respectively. We observed that when community population having the following characteristics as: living in Butuo country (OR=3.83), being males (OR=1.77), being Yi nationality (OR=4.40) being widowed (OR=28.57), with history of drug abuse (OR=3.71) or injecting drug use (PWID) (OR=4.92), or history of needle sharing among PWID (OR=8.53), were under higher risks for HIV infection. With histories as: having had secondary or above levels of schooling (OR=0.59), having protected sex with regular partners (OR=0.21) and with non-regular partners (OR=0.46), they seemed to be somehow protected for getting HIV infection. Conclusion: The positive HIV rates of HIV among community population in Dechang, Ningnan and Butuo varied from 0.10% to 8.77% while the HIV transmission among general population remained challenging.


Assuntos
Infecções por HIV/etiologia , Uso Comum de Agulhas e Seringas , Vigilância de Evento Sentinela , Abuso de Substâncias por Via Intravenosa/epidemiologia , China/epidemiologia , China/etnologia , Análise de Dados , HIV , Infecções por HIV/etnologia , Humanos , Masculino , Comportamento Sexual/etnologia , Abuso de Substâncias por Via Intravenosa/virologia , Inquéritos e Questionários
7.
Insect Mol Biol ; 26(6): 702-714, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28799241

RESUMO

Carboxylesterases (CarEs) play an important role in detoxifying insecticides in insects. Over-expression and structural modification of CarEs have been implicated in the development of organophosphate (OP) insecticide resistance in insects. A previous study identified four nonsynonymous mutations (resulting in four amino acid residue substitutions) in the open reading frame of the carboxylesterase gene of resistant cotton aphids compared to the omethoate susceptible strain, which has possibly influenced the development of resistance to omethoate (a systemic OP insecticide). The current study further characterized the function of these mutations, both alone and in combination, in the hydrolysis of OP insecticides. The metabolism results suggest that the combination of four mutations, mainly existing in the laboratory-selected OP-resistant cotton aphid population, increased the OP hydrolase activity (approximately twofold) at the cost of detectable carboxylesterase activity. The functional studies of single or multiple mutations suggest the positive effect of H104R, A128V and T333P on the acquisition of OP hydrolase activity, especially the combination of H104R with A128V or T333P. K484R substitution decreased both the OP hydrolase activity and the CarE activity, indicating that this mutation primarily drives the negative effect on the acquisition of OP hydrolase activity amongst these four mutations in the resistant strain. The modelling and docking results are basically consistent with the metabolic results, which strongly suggest that the structural gene modification is the molecular basis for the OP resistance in this laboratory-selected cotton aphid strain.


Assuntos
Afídeos/genética , Hidrolases de Éster Carboxílico/genética , Inseticidas , Organofosfatos , Animais , Afídeos/enzimologia , Linhagem Celular , Himecromona/análogos & derivados , Himecromona/metabolismo , Resistência a Inseticidas/genética , Simulação de Acoplamento Molecular , Mutação , Naftóis/metabolismo , Spodoptera
8.
Zhonghua Liu Xing Bing Xue Za Zhi ; 38(4): 486-490, 2017 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-28468068

RESUMO

Objective: To analyze the HIV-infection related behaviors among unmarried youths in rural areas of Liangshan prefecture from 2011 to 2013 and factors that influencing the HIV transmission in the population under research. Methods: According to the HIV sentinel surveillance methods for data collection, EpiData 3.1 and SPSS 19.0 were used to handle data and both Chi-square test and logistic regression were used to explore the related factors. Results: A total number of 5 871 qualified youths were involved in this study from 2011 to 2013 with the prevalence rate on HIV infection among those unmarried youths from the rural areas as 3.45% (201/5 833). 27.56% (1 607/5 833) of them had been working outside their hometown area. 31.72% (1 850/5 833) of the unmarried youths had sexual experiences, with 41.46% (767/1 850) of them had casual sex but the rate of consistent condom use was only 3.46% (64/1 850). 5.04% (294/5 833) of the unmarried youths admitted of ever having used drugs, with 34.35% (101/294) of them having the experiences of injecting, with 84.16% (85/101) of them sharing needles. Rates on factors as: working outside the resident areas, ever having had casual sexual behaviors, drug use and injecting drug use were seen higher among HIV infections, with differences statistically significant (χ(2)=88.72, 104.43, 4.20, 154.39, 55.94, P<0.05). Results from the logistic regression showed that factors as: being male,Yi ethnicity, illiteracy, experience of working outside the resident area, never or casual condom use and needle-sharing drug use etc. would significantly increase the risk of HIV infection. Conclusions: HIV infection among unmarried youths from rural areas in Liangshan prefecture called for attention because of the high rates of risk behaviors. Factors as: having had sexual experiences with low condom use, popular injecting drug use with needle sharing, being male, under Yi ethnicity, with lower education level and ever working outside the resident area etc. were under risks that related to HIV infection for unmarried youths in this area.


Assuntos
Infecções por HIV/prevenção & controle , Uso Comum de Agulhas e Seringas/estatística & dados numéricos , Comportamento Sexual , Pessoa Solteira/estatística & dados numéricos , Abuso de Substâncias por Via Intravenosa/epidemiologia , Adolescente , Adulto , China/epidemiologia , Grupos Étnicos , Feminino , Infecções por HIV/epidemiologia , Infecções por HIV/transmissão , Humanos , Modelos Logísticos , Masculino , Prevalência , Assunção de Riscos , População Rural , Sexo Seguro/estatística & dados numéricos , Vigilância de Evento Sentinela , Pessoa Solteira/psicologia , Abuso de Substâncias por Via Intravenosa/psicologia , Sexo sem Proteção/estatística & dados numéricos
9.
Andrologia ; 49(5)2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27862170

RESUMO

Azoospermia factor (AZF) genes on the long arm of the human Y chromosome are involved in spermatogenesis, and microdeletions in the AZF region have been recognised to be the second major genetic cause of spermatogenetic failure resulting in male infertility. While screening for these microdeletions can avoid unnecessary medical and surgical treatments, current methods are generally time-consuming. Therefore, we established a new method to detect and analyse microdeletions in the AZF region quickly, safely and efficiently. In total, 1,808 patients with spermatogenetic failure were recruited from three hospitals in southern China, of which 600 patients were randomly selected for screening for Y chromosome microdeletions in AZF regions employing real-time polymerase chain reaction with a TaqMan probe. In our study, of 1,808 infertile patients, 150 (8.3%) were found to bear microdeletions in the Y chromosome using multiplex PCR, while no deletions were found in the controls. Among the AZF deletions detected, two were in AZFa, three in AZFb, 35 in AZFc, three in AZFb+c and two in AZFa+b+c. Our method is fast-it permits the scanning of DNA from a patient in one and a half hours-and reliable, minimising the risk of cross-contamination and false-positive and false-negative results.


Assuntos
DNA/análise , Infertilidade Masculina/genética , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Adulto , Azoospermia/genética , China , Deleção Cromossômica , Cromossomos Humanos Y/genética , DNA/genética , Humanos , Masculino , Pessoa de Meia-Idade , Oligospermia/genética , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase em Tempo Real , Sensibilidade e Especificidade , Aberrações dos Cromossomos Sexuais , Espermatogênese/genética
10.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(6): 1006-1011, 2016 12 18.
Artigo em Chinês | MEDLINE | ID: mdl-27987505

RESUMO

OBJECTIVE: To investigate the early complication rate and identify patient-related independent clinical risk factors for early complications in patients following interventional pulmonology procedures. METHODS: In the period from December 2014 to December 2015, sufficient data of Peking University First Hospital Respiratory and Critical Care Medicine Department for analysis were identified in 218 subjects. Interventional pulmonology procedures were performed in all the patients. Early complications after the procedures were defined as newly respiratory failure, arrhythmia requiring treatment, severe hemoptysis, pneumothorax, pneumomediastinum, pulmonary edema, tracheoesophageal fistulae, bronchopleural fistulae, acute coronary syndrome, acute cerebrovascular accident, and death. Patient-related clinical risk factors were defined as coronary atherosclerotic heart disease, cerebral infarction, diabetes mellitus, cirrhosis, chronic kidney disease, arrhythmia, asthma, chronic obstructive pulmonary disease, hypertension, and previous interventional pulmonology treatment. The patient-related independent clinical risk factors which had close relations to the occurrence of early complications were analyzed by multivariate statistical analysis with Logistic regression. RESULTS: There were 56.4% male and 43.6% female subjects in this study. There were 10.6% current smokers, 26.6% former smokers, and 62.8% non-smokers. The overall early complication rate was 8.3%. In all the subjects groups, the patient-related independent clinical risk factors for the early complication rate were coronary atherosclerotic heart disease (B=1.545, P=0.006, OR=4.686, 95% CI 1.568-14.006), chronic obstructive pulmonary disease (B=1.037, P=0.049, OR=2.820, 95% CI 1.675-11.790), and current smoking status (B=1.412, P=0.032, OR=4.139, 95% CI 1.134-15.109); for the newly respiratory failure rates were coronary atherosclerotic heart disease (B=2.207, P=0.004, OR=9.087, 95% CI 2.028-40.714), chronic obstructive pulmonary disease (B=1.646, P=0.048, OR=5.188, 95% CI 1.783-34.375), and lesions involving three central airways (B=1.899, P=0.032, OR=6.680, 95% CI 1.182-37.740). In the malignant group, the patient-related independent clinical risk factor for the early complication rate was current smoking status (B=2.953, P=0.006, OR=19.161, 95% CI 2.360-155.572). In the benign group, the patient-related independent clinical risk factor for the early complication rate was only coronary atherosclerotic heart disease (B=1.976, P=0.022, OR=7.214, 95% CI 1.324-39.298). CONCLUSION: Closer monitoring of patients with identified clinical risk factors is advisable prior and immediately after interventional pulmonology procedures. In order to avoid or minimize early complications, special attention should be directed toward patients who are current smokers, or patients with lesions involving three central airways, or with coronary atherosclerotic heart disease or chronic obstructive pulmonary disease.


Assuntos
Complicações Pós-Operatórias/epidemiologia , Sistema Respiratório/cirurgia , Fatores de Risco , Síndrome Coronariana Aguda/etiologia , Arritmias Cardíacas/etiologia , Doença da Artéria Coronariana/complicações , Feminino , Hemoptise/etiologia , Humanos , Modelos Logísticos , Masculino , Enfisema Mediastínico/etiologia , Pneumotórax/etiologia , Complicações Pós-Operatórias/mortalidade , Doença Pulmonar Obstrutiva Crônica/complicações , Edema Pulmonar/etiologia , Insuficiência Respiratória/etiologia , Fumar/efeitos adversos , Acidente Vascular Cerebral/etiologia , Fístula Traqueoesofágica/etiologia
11.
Zhonghua Yi Xue Za Zhi ; 96(36): 2868-2871, 2016 Sep 27.
Artigo em Chinês | MEDLINE | ID: mdl-27760628

RESUMO

Objective: To evaluate the efficacy and safety of microsurgical crossover vasovasostomy in treating complicated obstructive azoospermia. Methods: The data of 14 patients with complicated obstructive azoospermia treated with microsurgical crossover vasovasostomy were reviewed from October 2012 to March 2016.Ten of them underwent microsurgical crossover vasovasostomy. Intraoperative exploration revealed that 2 patients had vas deferens injury and contralateral testicular atrophy or epididymal obstruction due to previous hernia repair; 7 patients had obstruction of intracorporeal vas deferens on one side and epididymal obstruction on the other side; the other 1 patient had unilateral vasal obstruction with contralateral epididymal obstruction. Furthermore, 4 patients underwent microsurgical crossover vasoepididymostomy, including 3 patients who had obstruction at caput epididymis on one side, and obstruction at cauda epididymis and distal vas deferens on the other side; the other patient had absence of vas deferens in the scrotum on one side, and testicular atrophy on the other side. Regular follow-up visits were conducted after the surgery. Results: Two patients were lost to follow-up; the other 12 patients were follow-up for an average of 11 (range: 2-23) months. In the 10 cases receiving microsurgical crossover vasovasostomy (including 2 patients lost to follow-up), 1 has not undergone semen re-analysis, 6 were confirmed patent, including 3 reporting spontaneous pregnancy. The patency rate in the 4 patients receiving microsurgical crosseover vasoepididymostomy was 2/4, with 1 patient reporting spontaneous pregnancy. There was no complaint of discomfort or complications following the surgery. Conclusions: Microsurgical crossover anastomosis may be effective and safe for patients with complicated obstructive azoospermia, according to preoperative assessment and intraoperative exploration. It allows natural conception for patients with refractory infertility. The microsurgical crossover anastomosis could be an effective therapy to achieve satisfactory patency of vas deferens.


Assuntos
Anastomose Cirúrgica , Azoospermia , Epididimo , Humanos , Masculino , Microcirurgia , Testículo , Ducto Deferente
12.
Zhonghua Liu Xing Bing Xue Za Zhi ; 37(8): 1105-7, 2016 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-27539341

RESUMO

OBJECTIVE: This paper aimed to analyze the situation of HIV infection in Liangshan Yi Autonomous, including HIV recent rate of infection and the ongoing strategies on prevention of the disease in this prefecture. METHODS: Surveillance data on population size, medical institutions, from 2011 to 2013 in Liangshan prefecture was collected. The total numbers of people living in the areas appeared as 412 608, 393 699 and 443 025 from the above mentioned three years. The overall numbers of people living with HIV/AIDS were 4 480, 3 999 and 4 719 respectively in the three years of observation, using the ELISA and WB blot. We also used the ELISA, and Western blotting methods to find out the HIV-positive individuals. We then applied the ' BED HIV-1 incidence capture enzyme immunoassay (BED-CEIA)' on these HIV-positive individuals to locate those who were recently infected, to estimate the annual recent infection rates. RESULTS: The HIV-positive rates in Liangshan Prefecture appeared as 1.09%, 1.02% and 1.07% respectively, from 2011 to 2013, with the recent infection rates as 0.34% (95%CI: 0.32-0.37), 0.62% (95%CI: 0.58-0.66) and 0.61% (95%CI: 0.57-0.65) respectively. The newly infections mainly involved with males, with Yi ethnicity, married, being illiterate or received primary school education. In males, the rates of infection appeared as 64.24% (433/674), 59.22% (504/851) and 58.37% (495/848) respectively, in the three years of observation among people with Yi ethnicity, the recent infection rates were 84.72% (571/674) in 2011, 94.59% (805/851) in 2012 and 84.20% (714/848) in 2013. The rates seen in married people were 61.57% (415/674), 61.45% (523/851) and 60.50% (513/848) respectively. Among the illiterates or people only received primary school education, the rates appeared as 60.24% (406/674) in 2011, 61.57% (524/851) in 2012, and 60.03% (509/848) in 2013. CONCLUSION: Using the HIV recent infection rate to describe the situation of AIDS in Liangshan prefecture, figures seemed to be more reliable than under the regular HIV-positive rate.


Assuntos
Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , HIV-1/imunologia , China/epidemiologia , Ensaio de Imunoadsorção Enzimática/métodos , Grupos Étnicos , Infecções por HIV/virologia , Humanos , Técnicas Imunoenzimáticas/métodos , Incidência , Masculino , Casamento , Fatores Socioeconômicos
13.
Public Health ; 139: 178-182, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27406975

RESUMO

OBJECTIVES: Metabolic syndrome (MS), as a precursor of diabetes mellitus (DM) and cardiovascular disease, is increasing steadily worldwide. We examined the preventive effects of lifestyle intervention on the occurrence of DM and acute myocardial infarction (AMI) in MS. STUDY DESIGN: Observational study on disease occurrence after lifestyle intervention. METHODS: The lifestyle intervention was administered to subjects with MS participating in a metropolitan lifestyle intervention program for 1 year. The same numbers of non-participating age- and sex-matched subjects with MS were randomly extracted from national health examination data. After intervention or examination, new occurrences of hypertension, DM, and AMI were identified through the national health insurance claims data during 1 year. For DM and AMI, multivariate logistic regression analysis for the factors affecting each disease was performed. RESULTS: In the intervention group and the control group (14,918 in each group), the occurrence of hypertension was 555 (6.07%) and 751 (8.33%), the occurrence of DM was 324 (2.55%) and 488 (3.89%), the occurrence of dyslipidemia was 321 (2.59%) and 373 (2.72%), and the occurrence of AMI was 13 (0.09%) and 26 (0.17%), respectively. In multivariate logistic regression analysis, adjusted odds ratios for intervention were 0.752 (95% confidence interval [CI]: 0.644-0.879) and 0.499 (95% CI: 0.251-0.992) for DM and AMI, respectively, indicating that lifestyle intervention has a preventive effect. CONCLUSIONS: Lifestyle intervention in MS has preventive effects on the occurrence of DM and AMI, and long-term follow-up is needed to evaluate these preventive effects in more detail.


Assuntos
Diabetes Mellitus/epidemiologia , Estilo de Vida , Síndrome Metabólica/epidemiologia , Infarto do Miocárdio/epidemiologia , Serviços Preventivos de Saúde , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Avaliação de Programas e Projetos de Saúde , República da Coreia/epidemiologia
14.
Clin Exp Obstet Gynecol ; 43(1): 134-6, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27048036

RESUMO

The laparoscopic subtotal hysterectomy (LSH) was given to a patient whose uterus was about seven-month pregnanacy because of fibroids. The biggest problem was the operation space and visual field was too narrow. Different from the usual procedure we do, we morcellated the uterus at the beginning to expand the space. Loop ligature of the uterine isthmus was adopted to block uterine ateries before morcellating the uterus. After the adnexa exposed totally, we started to cut off the round ligaments, proper ligaments and fallopian tubes like usual. It was the first time we did LSH for so giant uterus in our hospital, although which was usually suitable for the uterus smaller than four-month pregnancy. But if the uterine ateries can be blocked effectively at the beginning, the uterus can be morcellated and the space will be enlarged. The laparoscopic subtotal hysterectomy will also be completed successfully.


Assuntos
Histerectomia/métodos , Laparoscopia/métodos , Leiomioma/cirurgia , Neoplasias Uterinas/cirurgia , Animais , Feminino , Humanos , Leiomioma/diagnóstico , Pessoa de Meia-Idade , Gravidez , Índice de Gravidade de Doença , Neoplasias Uterinas/diagnóstico
15.
Epidemiol Infect ; 144(6): 1291-300, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26542331

RESUMO

Hepatitis C virus (HCV) has become a global public health problem. Many studies have been conducted to identify risk factors for HCV infection. However, some of these studies reported inconsistent results. Using data collected from 11 methadone clinics, we fit both a non-spatial logistical regression and a geographically weighted logistic regression to analyse the association between HCV infection and some factors at the individual level. This study enrolled 5401 patients with 30·0% HCV infection prevalence. The non-spatial logistical regression found that injection history, drug rehabilitation history and senior high-school education or above were related to HCV infection; and being married was negatively associated with HCV infection. Using the spatial model, we found that Yi ethnicity was negatively related to HCV infection in 62·0% of townships, and being married was negatively associated with HCV infection in 81·0% of townships. Senior high-school education or above was positively associated with HCV infection in 55·2% of townships of the Yi Autonomous Prefecture. The spatial model offers better understanding of the geographical variations of the risk factors associated with HCV infection. The geographical variations may be useful for customizing intervention strategies for local regions for more efficient allocation of limited resources to control transmission of HCV.


Assuntos
Usuários de Drogas/estatística & dados numéricos , Hepacivirus/fisiologia , Hepatite C/epidemiologia , Hepatite C/etiologia , Abuso de Substâncias por Via Intravenosa/complicações , Abuso de Substâncias por Via Intravenosa/epidemiologia , Adulto , Idoso , China/epidemiologia , Demografia , Feminino , Geografia , Hepatite C/virologia , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Fatores Socioeconômicos , Adulto Jovem
16.
Eur Rev Med Pharmacol Sci ; 19(21): 4035-46, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26592825

RESUMO

OBJECTIVE: Induced pluripotent stem cells (iPSCs) have emerged as a promising tool for treating incurable diseases. The current challenges are to avoid potential xenopathogenic transmission and immune rejection potentially caused by exposure of iPSCs to animal-derived products. In addition, an efficient feeder cell-free culture condition will be required for minimizing batch-to-batch variation and facilitating scale-up. Therefore, establishing an efficient extracellular matrix (ECM) culture system is considered as a prerequisite for the future clinical application of iPSC-based cell therapies. In this study, we evaluated the feasibility of culturing iPSCs in ECM derived from human dental pulp cells (hDCP). MATERIALS AND METHODS: iPSCs growing in Matrigel were transferred to ECM or Matrigel and cultured in mTeSRTM1 medium. RESULTS: The number of adherent cells in the ECM group was higher than that in the Matrigel group after incubation for 8, 12, and 24 h, indicating that the ECM could enhance cell adherence. The adhesion of cells to ECM not only depends on simple physical attachment with ECM, but also mediated by fibronectin in the ECM. The hDPC-iPSCs showed orderly growth in the ECM, suggesting that the ECM could promote the growth and proliferation of hDPC-iPSCs. We also observed that stem cells growed along to avoid contact inhibition. CONCLUSIONS: The iPSCs maintained undifferentiated state when cultured in ECM when the iPSCs and ECM are of the same cell origin. ECM and mTeSRTM1, can both be used as new culture medium for iPSCs that facilitates the clinical application of iPSC-based cell therapies in the future.


Assuntos
Técnicas de Cultura de Células/métodos , Diferenciação Celular/fisiologia , Polpa Dentária/citologia , Matriz Extracelular/fisiologia , Células-Tronco Pluripotentes Induzidas/fisiologia , Adolescente , Diferenciação Celular/efeitos dos fármacos , Colágeno/química , Colágeno/farmacologia , Meios de Cultura/farmacologia , Combinação de Medicamentos , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/fisiologia , Matriz Extracelular/química , Matriz Extracelular/efeitos dos fármacos , Humanos , Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos , Laminina/química , Laminina/farmacologia , Masculino , Proteoglicanas/química , Proteoglicanas/farmacologia , Tecidos Suporte/química
17.
Eur Rev Med Pharmacol Sci ; 19(3): 412-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25720712

RESUMO

OBJECTIVE: We performed this study to investigate the effect of blood pressure control in ultra-early basal ganglia intracerebral hemorrhage. PATIENTS AND METHODS: 120 patients with ultra-early basal ganglia intracerebral hemorrhage were randomly divided into experimental group (strengthened antihypertensive) and control (normal antihypertensive). Each group consists of 60 patients, whose contractive pressure were controlled by intravenous antihypertensive drugs among 130-140 mmHg and 160-180 mmHg respectively for 24 h, after 1 h of beginning treatment. They were all evaluated by NIH Stroke Scale (NIHSS) before and after the treatment. Cranial CT, hematoma volume, hematoma enlargement, edema volume, serum matrix metalloproteinase-9 level were performed and compared between groups. RESULTS: After 24 h, hematoma volume and hematoma enlargement in the experimental group was significantly lower than control (p < 0.05). After 14 days, NIHSS score in the experimental group was significantly lower than control (p < 0.05). Cerebral edema amount and serum MMP-9 level in the experimental group were significantly lower than control after 5 days and 14 days. CONCLUSIONS: Ultra-early basal ganglia intracerebral hemorrhage can remarkably reduce hematoma enlargement, cerebral edema, serum MMP-9 level, and improve the neurological function.


Assuntos
Anti-Hipertensivos/administração & dosagem , Gânglios da Base/patologia , Pressão Sanguínea/efeitos dos fármacos , Pressão Sanguínea/fisiologia , Hemorragia Cerebral/diagnóstico , Hemorragia Cerebral/tratamento farmacológico , Idoso , Gânglios da Base/efeitos dos fármacos , Edema Encefálico/sangue , Edema Encefálico/diagnóstico , Edema Encefálico/tratamento farmacológico , Hemorragia Cerebral/sangue , Feminino , Hematoma/sangue , Hematoma/diagnóstico , Hematoma/tratamento farmacológico , Humanos , Infusões Intravenosas , Masculino , Metaloproteinase 9 da Matriz/sangue , Pessoa de Meia-Idade , Fatores de Tempo
18.
Andrologia ; 46(5): 504-12, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23682825

RESUMO

This study was conducted to investigate the effects of aescin treatment in a rodent model treated with an experimentally induced varicocele. Experimental varicocele was induced by partial ligation of the left renal vein of rats. Aescin administration was performed daily for 4 weeks after the varicocele induction. Seven weeks later, a contrast-enhanced ultrasound was performed of the rats' testis to assess testicular blood flow. The animals were sacrificed, and H&E staining was then used to evaluate testicular pathological changes and polymorphonuclear leucocytes density. Cauda epididymal sperm counts and motility were evaluated. Blood was collected for the measurement of follicle-stimulating hormone, luteinising hormone and testosterone. Contrast-enhanced ultrasound showed that there were significant decreases in testicular blood flow in the aescin-treated groups compared with those in control varicocele group. Testicular oedema was detected in those rats treated with a varicocele but without aescin, while no oedema was found in the experimental group. H&E staining showed dysfunctional spermatogenesis in both cohorts; however, polymorphonuclear leucocytes density was significantly reduced in aescin-treated groups. There was an increase in sperm counts of the aescin-treated groups. Our study demonstrated that aescin could exert therapeutical effects on reversal of testicular lesions in varicocele rats.


Assuntos
Escina/farmacologia , Testículo/efeitos dos fármacos , Varicocele/patologia , Animais , Hormônio Foliculoestimulante/sangue , Masculino , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , Motilidade Espermática , Testículo/patologia
19.
Water Sci Technol ; 62(6): 1320-6, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20861546

RESUMO

A mesoporous SBA-15 doped iron oxide (Fe2O3/SBA-15) was synthesized by co-condensation, characterized and used as heterogeneous catalysts for the photo-Fenton decolorization of azo dye Orange II under UV irradiation. Response surface methodology (RSM) was used to investigate operating condition effects, such as hydrogen peroxide concentration, initial pH and catalyst loadings, on the decolorization rate. UV irradiation is found to enhance the activity of the catalyst in the process. RSM analysis evidenced the influence of the initial pH value and H2O2 concentration on the dye degradation rate. The coupled UV/Fe2O3/SBA-15/H2O2 process at room temperature is revealed as a promising friendly process for wastewater treatment. Indeed, the use of a heterogeneous catalyst allows an easy active phase recycling without multi-step recovering while the heterogeneous catalyst used here exhibits high catalytic activity for the reaction considered.


Assuntos
Compostos Azo/análise , Benzenossulfonatos/análise , Peróxido de Hidrogênio/química , Ferro/química , Modelos Teóricos , Raios Ultravioleta , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Análise de Variância , Compostos Azo/efeitos da radiação , Benzenossulfonatos/efeitos da radiação , Catálise , Gráficos por Computador , Compostos Férricos/química , Oxirredução , Porosidade , Dióxido de Silício/química , Poluentes Químicos da Água/efeitos da radiação
20.
Eur J Clin Microbiol Infect Dis ; 29(7): 907-11, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20440530

RESUMO

Two detection methods for H. pylori infections, i.e. serological antibody titer measurements by Hp-ELISA and histological scoring by HE staining, have been compared to investigate the relationship between the diagnosis methods, to clarify the application scope of each diagnosis method and to determine its influencing factors. In the 7,241 subjects who participated in gastric cancer screening, H. pylori infection rate was 70.14% by the Hp-HE method and 41.87% by the Hp-ELISA method when 34EIU was recognized as the cut-off value. The IgG titers increased with the elevation of HE scores; however, the two methods were not closely correlated among those in different gastric disease status. Age, gender and drinking status did not have significant impact on the relationship between the two methods; however, smoking status seemed to significantly influence the correlation of the two diagnosis methods. In conclusion, it was necessary to reevaluate the cut-off value when using ELISA test kits in different population groups. In most cases, the results of two H. pylori infection diagnosis methods show high correlation. However, this relationship can be affected by smoking and gastric diseases status. Additionally, the dynamic change of H. pylori antibody titers is an indicator of gastric disease development.


Assuntos
Técnicas Bacteriológicas/métodos , Infecções por Helicobacter/diagnóstico , Helicobacter pylori/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antibacterianos/sangue , Criança , Pré-Escolar , China , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Infecções por Helicobacter/microbiologia , Infecções por Helicobacter/patologia , Helicobacter pylori/imunologia , Histocitoquímica/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA