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1.
Dalton Trans ; 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32196064

RESUMO

Two FeII8L12 cubic metal-organic cages were constructed with semi-rigid ligands and they further self-assembled into supramolecular assemblies with three different porous cavities. The supramolecular assemblies showed synergistic adsorption of I2 and TTF, and their solid state spin-crossover behaviors were influenced by the adsorbed guest molecules.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32182951

RESUMO

According to the World Health Organization (WHO), one out of 10 people get sick from eating contaminated food. Complex food production process and globalization make food supply chain more delicate. Many technologies have been investigated in recent years to address food insecurity and achieve efficiency in dealing with food recalls. One of the most promising technologies is Blockchain, which has already been used successfully in financial aspects, such as bitcoin, and it is attracting interests from food supply chain organizations. As blockchain has characteristics, such as decentralization, security, immutability, smart contract, it is therefore expected to improve sustainable food supply chain management and food traceability. This paper applies a content-analysis based literature review in blockchain adoption within food supply chain. We propose four benefits. Blockchain can help to improve food traceability, information transparency, and recall efficiency; it can also be combined with Internet of things (IoT) to achieve better efficiency. We also propose five potential challenges, including lack of deeper understanding of blockchain, technology difficulties, raw data manipulation, difficulties of getting all stakeholders on board, and the deficiency of regulations.

3.
Inorg Chem ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32191464

RESUMO

The AlF2(O2S) and SiF2(O2S) complexes with bidentately coordinated sulfur dioxide ligands were prepared via the reactions of aluminum and silicon atoms with SO2F2 under UV-vis irradiation in cryogenic matrixes. The products were identified by infrared spectroscopy as well as DFT frequency calculations, and nonplanar C2v structures with the SO2 ligand bound to Al/Si via two oxygen atoms were established. In spite of the similarity in geometric structures, the electronic structures of AlF2(O2S) and SiF2(O2S) are completely different. The longer S-O bond length and the lower O-S-O stretching vibrational frequencies of SiF2(O2S) in comparison to those of AlF2(O2S) indicate that SiF2(O2S) possesses a singlet SO22--like ligand while the SO2 ligand is better described as SO2- in AlF2(O2S), which is further confirmed by bonding and population analysis. Linear correlations were established between the experimental O-S-O stretching frequencies and calculated S-O bond lengths of SO2, AlF2(O2S), and SiF2(O2S), suggesting that the S-O bond is gradually activated when SO2 is coordinated to AlF2 and SiF2.

4.
Emerg Infect Dis ; 26(4): 711-720, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32186492

RESUMO

Incidence of invasive pneumococcal disease caused by antimicrobial-resistant Streptococcus pneumoniae types not included in pneumococcal conjugate vaccines has increased, including a penicillin- and meropenem-resistant serotype 15A-ST63 clone in Japan. During 2013-2017, we collected 206 invasive pneumococcal isolates in Taiwan for penicillin and meropenem susceptibility testing. We found serotypes 15B/C-ST83 and 15A-ST63 were the most prevalent penicillin- and meropenem-resistant clones. A transformation study confirmed that penicillin-binding protein (PBP) 2b was the primary meropenem resistance determinant, and PBP1a was essential for high-level resistance. The rate of serotype 15B/C-ST83 increased during the study. All 15B/C-ST83 isolates showed an ermB macrolide resistance genotype. Prediction analysis of recombination sites revealed 12 recombination regions in 15B/C-ST83 compared with the S. pneumoniae Spain23F-ST81 genome. Pneumococcal clones rapidly recombine to acquire survival advantages and undergo local expansion under the selective pressure exerted by vaccines and antimicrobial drugs. The spread of 15B/C-ST83 is alarming for countries with high antimicrobial pressure.

5.
Dalton Trans ; 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32101238

RESUMO

Gas-phase coordination complexes of actinyl(v) cations, AnO2+, provide a basis to assess fundamental aspects of actinide chemistry. Electrospray ionization of solutions containing an actinyl cation and sulfonate anion CH3SO2- or C6H5SO2- generated complexes [(AnVO2)(CH3SO2)2]- or [(AnVO2)(C6H5SO2)2]- where An = Np or Pu. Collision induced dissociation resulted in C-S bond cleavage for methanesulfinate to yield [(AnVO2)(CH3SO2)(SO2)]-, whereas hydrolytic ligand elimination occurred for benzenesulfinate to yield [(AnVO2)(C6H5SO2)(OH)]-. These different fragmentation pathways are attributed to a stronger C6H5-SO2- versus CH3-SO2- bond, which was confirmed for both the bare and coordinating sulfinate anions by energies computed using a relativistic multireference perturbative approach (XMS-CASPT2 with spin-orbit coupling). The results demonstrate shutting off a ligand fragmentation channel by increasing the strength of a particular bond, here a sulfinate C-S bond. The [(AnVO2)(CH3SO2)(SO2)]- complexes produced by CID spontaneously react with O2 to eliminate SO2, yielding [(AnO2)(CH3SO2)(O2)]-, a process previously reported for An = U and found here for An = Np and Pu. Computations confirm that the O2/SO2 displacement reactions should be exothermic or thermoneutral for all three An, as was experimentally established. The computations furthermore reveal that the products are superoxides [(AnVO2)(CH3SO2)(O2)]- for An = Np and Pu, but peroxide [(UVIO2)(CH3SO2)(O2)]-. Distinctive reduction of O2- to O22- concomitant with oxidation of U(v) to U(vi) reflects the relatively higher stability of hexavalent uranium versus neptunium and plutonium.

6.
J Cell Physiol ; 2020 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-32037554

RESUMO

Endothelial glycocalyx degradation, critical for increased pulmonary vascular permeability, is thought to facilitate the development of sepsis into the multiple organ failure. Maresin conjugates in tissue regeneration 1 (MCTR1), a macrophage-derived lipid mediator, which exhibits potentially beneficial effects via the regulation of bacterial phagocytosis, promotion of inflammation resolution, and regeneration of tissue. In this study, we show that MCTR1 (100 ng/mouse) enhances the survival of mice with lipopolysaccharide (LPS)-induced (15 mg/kg) sepsis. MCTR1 alleviates LPS (10 mg/kg)-induced lung dysfunction and lung tissue inflammatory response by decreasing inflammatory cytokines (tumor necrosis factor-α, interleukin-1ß [IL-1ß], and IL-6) expression in serum and reducing the serum levels of heparan sulfate (HS) and syndecan-1. In human umbilical vein endothelial cells (HUVECs) experiments, MCTR1 (100 nM) was added to the culture medium with LPS for 6 hr. MCTR1 treatment markedly inhibited HS degradation by downregulating heparanase (HPA) protein expression in vivo and in vitro. Further analyses indicated that MCTR1 upregulates sirtuin 1 (SIRT1) expression and decreases NF-κB p65 phosphorylation. In the presence of BOC-2 or EX527, the above effects of MCTR1 were abolished. These results suggest that MCTR1 protects against LPS-induced sepsis in mice by attenuating pulmonary endothelial glycocalyx injury via the ALX/SIRT1/NF-κB/HPA pathway.

7.
Sci Total Environ ; 714: 136648, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32018951

RESUMO

Land-use conversion of pristine boreal peatlands for agricultural purposes is an ongoing process and projected to become more intensive with rising population growth and increased demands for food production. However, agricultural use of peatlands affects the production and emission of nitrous oxide (N2O), a very potent greenhouse gas currently gaining more attention in the global assessment of greenhouse gases. While the intensity of N2O emissions depends on a range of environmental factors, including hydrological parameters, temperature and the availability of nitrogen in soils, key driving processes remain poorly understood. In order to understand the effects of land-use change on the peatland ecosystem, we quantified N2O fluxes under different land-use in a comparative study between a natural bog and an adjacent abandoned pasture in Newfoundland, Canada. We conducted in situ gas flux measurements using the static chamber method over five growing seasons. In addition, we measured photosynthetic rates and environmental parameters, namely soil temperature and moisture, water table and concentrations of total nitrogen and dissolved organic carbon in pore waters. According to previous studies, we hypothesized higher N2O emissions from the abandoned pasture due to drainage compared to the natural bog. However, despite significant differences of environmental parameters and photosynthetic rates, we found no significant difference of N2O fluxes between the two sites. We argue that N2O production at the abandoned pasture was inhibited due to exhaustion of plant-available nitrogen as a result of increased gross primary production compared to the natural bog. We conclude that the effect of drainage and fertilization on N2O fluxes during the growing season was superposed by vegetation composition change effects at the abandoned pasture, leading to similar N2O fluxes at both sites.

8.
J Phys Condens Matter ; 32(16): 165702, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910400

RESUMO

Tungsten tetraboride (WB4)-based solid solutions represent one of the most promising superhard metal candidates; however, their underlying hardening mechanisms have not yet been fully understood. Here, we explore the lattice compressibility of WB4 binary solid solutions with different manganese (Mn) concentrations using high-pressure x-ray diffraction (XRD) up to 52 GPa. Under initial compression, the lattices of low and high Mn-doped WB4 alloys (i.e. W0.96Mn0.04B4 and W0.84Mn0.16B4) are shown to be more and less compressible than pure WB4, respectively. Then, a c-axis softening is found to occur above 39 GPa in WB4, consistent with previous results. However, an anomalous sudden a-axis stiffening is revealed at ~36 GPa in W0.96Mn0.04B4, along with suppression of c-axis softening observed in WB4. Furthermore, upon Mn addition, a simultaneous stiffening of a- and c-axes is demonstrated in W0.84Mn0.16B4 at ~37 GPa. Speculation on the possible relationship between this anomalous stiffening and the combined effects of valence-electron concentration (VEC) and atomic size mismatch is also included to understand the origin of the nearly identical hardness enhancement in those two solid solutions compared to WB4. Our findings emphasize the importance of accurate bonding and structure manipulation via solute atoms to best optimize the hardness of WB4 solid solutions.

9.
Mol Biol Rep ; 47(2): 1057-1065, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31802368

RESUMO

Watermelon (Citrullus lanatus) is one of the major cucurbit crop that cultivated all over the world. Adaptability and flowering time are important agronomic characteristics that influence the quality and yield of watermelon, however, the molecular basis underlying these traits were still unclear. In this study, we identified 166, 182, 178, and 279 flowering genes in watermelon, melon, cucumber and pumpkin, respectively, and found that a lot of genes in the photoperiodic, autonomous, and vernalization pathways were absence in the four cucurbits. A higher ratio of flowering time genes was identified in the hormone pathway in cucurbits than in Arabidopsis, and a higher average ka/ks value of hormone pathway genes than the photoperiodic and vernalization pathway genes was identified in watermelon. Moreover, a gene ClGA2/KS (Cla005482) were found to associated with ecotype differentiation, flowering time, and whole growth period in watermelon. This study added knowledge to the molecular basis of flowering time regulation in cucurbits, and the molecule marker of ClGA2/KS gene may facilitate the breeding progress for selecting watermelon varieties with superior adaption and flowering time.

10.
J Cell Physiol ; 235(2): 1013-1024, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31240715

RESUMO

Iron is an essential metal ion in the human body and usually dysregulated in cancers. However, a comprehensive overview of the iron-related genes and their clinical relevance in cancer is lacking. In this study, we utilized the expression profiling, proteomics, and epigenetics from the Cancer Genome Atlas database to systematically characterized the alterations of iron-related genes. There were multiple iron-related genes with dysregulation across 14 cancers and some of these ectopic changes may be associated with aberrant DNA methylation. Meanwhile, a variety of genes were significantly associated with patient survival, especially in kidney renal clear cell carcinoma. Then differentially expressed genes were validated in clinical samples. Finally, we found deferoxamine and erastin could inhibit proliferation in various tumor cells and influence the expression of several iron-related genes. Overall, our study provides a comprehensive analysis of iron metabolism across cancers and highlights the potential treatment of iron targeted therapies for cancers.

11.
Clin Interv Aging ; 14: 1763-1769, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695346

RESUMO

Background: Critically ill older patients with acute kidney injury (AKI), also referred to as acute renal failure, are associated with high in-hospital mortalities. Preexisting malnutrition is highly prevalent among AKI patients and increases in-hospital mortality rate. This study is to evaluate the predictive power of some serum nutritional related biomarkers predicting the 90 days in-hospital mortality of critically ill older patients with AKI. Methods: A prospective, observational study was conducted in a university teaching hospital. One hundred and five critically ill older patients with AKI aged 60-95 were enrolled and were divided into survival group (n=44) and non-survival group (n=61) in the light of their final outcomes. Receiver operating characteristic analyses (ROC) were performed to calculate the area under ROC curve (AUC). Sensitivity and specificity of in-hospital mortality prediction were calculated. Results: Significant differences were found between the survival group and non-survival group of critically ill older patients with AKI. AUC of low density lipoprotein (LDL) and albumin were 0.686 and 0.595, respectively. The asymptotic 95% confidence intervals of LDL and albumin were 0.524-0.820 and 0.488-0.696, respectively. Sensitivity of the 90 days in-hospital mortality prediction of LDL and albumin were 68.71% and 69.09%, respectively. Specificity of 90 days in-hospital mortality prediction of LDL and albumin were 69.23% and 50.0%, respectively. Conclusion: LDL and albumin did not have sufficient power to predict the 90 days in-hospital mortality of critically ill older patients with AKI. Further research on the association between malnutrition and poor prognosis of critically ill older patients with AKI is needed in the future.Trial registration: ClinicalTrials.gov identifier: NCT00953992.


Assuntos
Lesão Renal Aguda/sangue , Lesão Renal Aguda/mortalidade , Mortalidade Hospitalar , Lipoproteínas LDL/sangue , Albumina Sérica/metabolismo , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Estado Terminal/mortalidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Curva ROC
12.
Australas J Dermatol ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31724160

RESUMO

Phaeohyphomycosis, caused by opportunistic rare fungi, has increasingly been reported in diabetic and immunosuppressed patients. Pyrenochaeta unguis-hominis (P. unguis-hominis), a member of the dematiaceae group, has been identified to cause human nail infection. We report a rare case of cutaneous phaeohyphomycosis in a diabetic female caused by P. unguis-hominis.

13.
Dalton Trans ; 48(46): 17158-17162, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31740908

RESUMO

A dinuclear Eu(iii) complex Eu2(OCH2NMe2)2[N(SiMe3)2]4 (1) was synthesized via the reaction of Me3NO and Eu[N(SiMe3)2]3, during which the unprecedented C-H activation of Me3NO occurred along with oxygen-atom transfer. A simple Eu[N(SiMe3)2]3(OPMe3) adduct (2) was obtained when Me3PO was used, which should be due to the higher energy barrier and poorer product stability that prevent the formation of the phosphine substituted analogue of 1.

14.
Math Biosci Eng ; 16(6): 7546-7561, 2019 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-31698628

RESUMO

Medical ultrasound images are corrupted by speckle noise, and despeckling methods are required to effectively and efficiently reduce speckle noise while simultaneously preserving details of tissues. This paper proposes a despeckling approach named the Gabor-based anisotropic diffusion coupled with the lattice Boltzmann method (GAD-LBM), which uses the lattice Boltzmann method (LBM) to fast solve the partial differential equation of an anisotropic diffusion model embedded with the Gabor edge detector. We evaluated the GAD-LBM on both synthetic and clinical ultrasound images, and the experimental results suggested that the GAD-LBM was superior to other nine methods in speckle suppression and detail preservation. For synthetic and clinical images, the computation time of the GAD-LBM was about 1/90 to 1/20 of the GAD solved with the finite difference, indicating the advantage of the GAD-LBM in efficiency. The GAD-LBM not only has excellent ability of noise reduction and detail preservation for ultrasound images, but also has advantages in computational efficiency.

15.
J Am Soc Mass Spectrom ; 30(12): 2623-2631, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31637665

RESUMO

Tetrapositive hafnium complexes in the form of Hf(TMPDA)34+ and Hf(TMOGA)34+ were produced by ESI of acetonitrile solutions of Hf(ClO4)4/TMPDA and Hf(ClO4)4/TMOGA respectively. Analogous Hf(TMGA)34+ and Hf(TMTDA)34+ were not observed when the Hf(ClO4)4/TMGA and Hf(ClO4)4/TMTDA solutions were subjected to ESI under similar conditions. Geometry optimizations on these four tetrapositive complexes revealed that the Hf(TMPDA)34+ and Hf(TMOGA)34+ complexes possess C3 and D3 geometries respectively with the Hf4+ center coordinated by nine atoms. Similar geometries were found for Hf(TMGA)34+ and Hf(TMTDA)34+, but both are six-coordinate complexes, which should account for their absence in the gas phase. In addition, no tetrapositive hafnium ion was observed when methanol was used as a solvent instead of acetonitrile. The much stronger affinity of Cl- toward Hf4+ than ClO4- should be the reason why tetrapositive hafnium ions were not observed when HfCl4 was used as the hafnium source. CID of the Hf(TMPDA)34+ and Hf(TMOGA)34+ complexes resulted in the formation of Hf(TMPDA)(TMPDA-H)3+ and Hf(TMOGA)(TMOGA-H)3+ respectively as the major products. The most stable structures of both tripositive hafnium products arise from the deprotonation of CH3 cis to Ocarbonyl, and the Hf(IV) center in both cases is six coordinate. Compared with the loss of protonated ligand observed in the experiments, it is much higher in energy for either Hf(TMPDA)34+ or Hf(TMOGA)34+ to lose neutral or cationic ligand on the basis of DFT calculations.

16.
Inorg Chem ; 58(22): 15652-15658, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31661249

RESUMO

Peroxo-like sulfur monoxide complexes of molybdenum and tungsten oxyfluorides [OMF2(η2-SO)] were prepared via the reactions of molybdenum and tungsten atoms and SO2F2 in cryogenic matrixes. On the basis of the infrared spectra and density functional theory calculations, the SO ligand is bound to the metal center in a side-on fashion, and both complexes possess closed shell singlet ground states. The experimental S-O stretching frequencies of OMoF2(η2-SO) and OWF2(η2-SO) are much lower than those of SO- but close to that of singlet SO2-, indicating that the SO ligand should be considered as SO2-. This is consistent with the rather long S-O bond length in comparison to that of the ONbF2(η2-SO) and OTaF2(η2-SO) complexes with SO- ligand. Bonding analysis results reveal that the π* (3π) orbitals of the triplet SO molecule are both doubly occupied in OMoF2(η2-SO) and OWF2(η2-SO) due to the two-electron transfer from metal to SO. End-on isomers with M-OS or M-SO geometries were also predicted to be stable, but all of them are higher in energy than the side-on complexes, and the calculated frequencies are inconsistent with the experimental values. Comparisons in bond lengths, vibrational frequencies, and natural charges between the OMF2(η2-SO) and OMF2(η2-O2) complexes (M = Mo, W, Nb, Ta) further confirm the peroxo-like character of the SO ligand in OMoF2(η2-SO) and OWF2(η2-SO).

17.
Life Sci ; 239: 116946, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610202

RESUMO

AIMS: Infantile hemangioma (IH) is the most common vascular neoplasm in infant and young children. Long non-coding RNAs (lncRNAs) are known to be associated with IH. This study aims to investigate the role and underlying mechanism of lncRNA-MALAT1 in IH. MAIN METHODS: qRT-PCR was used to quantify the expressions of MALAT1, miR-424, and MEKK3 in IH tissues. The cell proliferation, apoptosis, migration, invasion, and tube formation ability were assessed by MTT assay, colony formation assay, flow cytometric analysis, transwell assay and tube formation assay, respectively. The interaction among MALAT1, miR-424 and MEKK3 was evaluated by luciferase reporter assay. Immunohistochemistry (IHC) and Western blotting were utilized to evaluate the expression levels of MEKK3, Ki-67 and NF-κB pathway-related proteins both in vitro and in vivo. KEY FINDINGS: In IH tissues, MALAT1 and MEKK3 were overexpressed while miR-424 was down-regulated. Silencing MALAT1 or overexpression of miR-424 significantly inhibited the IH cell proliferation, migration and tube formation, but promoted the cell apoptosis. Knockdown of MALAT1 suppressed the expression of MEKK3 and inactivated the IKK/NF-κB pathway by sponging miR-424. Overexpression of MEKK3 in HemEcs reversed the impact of knockdown of MALAT1 and overexpression of miR-424 on the cell proliferation, apoptosis, migration, invasion and tube formation rate. The tumor xenografts experiments demonstrated that silencing MALAT1 significantly inhibited the tumor growth in vivo and Ki-67 in the tumor tissues was also significantly suppressed. SIGNIFICANCE: MALAT1 promoted the IH progression through inhibiting miR-424 to activate MEKK3-mediated IKK/NF-κB pathway, suggesting that MALAT1, miR-424 and MEKK3 could be used as potential targets to improve IH treatment efficiency.


Assuntos
Hemangioma/metabolismo , MAP Quinase Quinase Quinase 3/metabolismo , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Animais , Apoptose/genética , Carcinogênese/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Feminino , Hemangioma/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , MicroRNAs/genética , NF-kappa B/metabolismo , Invasividade Neoplásica , RNA Longo não Codificante/genética , RNA Interferente Pequeno/genética , Transdução de Sinais , Ensaios Antitumorais Modelo de Xenoenxerto
18.
Exp Ther Med ; 18(5): 4011-4021, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31611939

RESUMO

Psoriasis is a chronic inflammatory skin disorder that impairs the quality of life of affected patients. Emerging studies indicate that certain long non-coding RNAs (lncRNAs) have important roles in psoriasis. However, the exact functions of lncRNAs and their regulatory mechanisms as competitive endogenous RNAs (ceRNAs) in psoriasis have remained to be fully elucidated. In the present study, differentially expressed lncRNAs, microRNAs (miRNAs) and mRNAs were identified by analyzing public datasets, and a psoriasis-associated lncRNA-miRNA-mRNA network was constructed based on the ceRNA theory. Furthermore, previously validated abnormally expressed miRNAs in psoriasis were identified by a systematic literature search in the PubMed and Web of Science databases, and a specific miRNA-associated lncRNA-miRNA-mRNA sub-network was extracted. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using DAVID 6.8. A total of 253 lncRNAs, 106 miRNAs and 1,156 mRNAs were identified as being differentially expressed between psoriasis skin and healthy control skin. The present study identified two key lncRNAs that may potentially have a role in the pathogenesis of psoriasis: AL035425.3 and Prader Willi/Angelman region RNA 6. This integrative analysis enhances the understanding of the molecular mechanism of psoriasis and may provide novel therapeutic targets for the treatment of psoriasis.

19.
Fish Shellfish Immunol ; 94: 833-841, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31580936

RESUMO

Glycoprotein (G) is the most common gene used in SVCV vaccine constructions. To identify the major immunogenicity determinant region of SVCV G gene, herein we truncated G gene to 4 parts (G-1, G-2, G-3 and G-4). Bioinformatics and the enzyme linked immunosorbent assay (ELISA) were used to identify the antigenicity of these 4 truncated G proteins. Immunological assays (serum antibody production, enzyme activity, immune genes expression and challenge test) were carried out to further identify the immunogenicity of the screened G protein in common carp. Moreover, to further verify the immune response of the screened G protein-based subunit vaccine, its protective effects on common carp against SVCV infection using single-walled carbon nanotubes (SWCNTs) as a carrier were evaluated. Results showed that G-3 protein could induce higher antibody titer than other truncated G proteins. Furthermore, carps vaccinated with G-3 and G (positive control) showed significant enhancement of immune response (serum antibody production, enzyme activity and immune related genes expression) when compared with control groups. Meanwhile, as a promising vaccine carrier, SWCNTs could significantly enhance the immune effect of naked subunit vaccine (G-3 and G). Notably, after SVCV challenge, there was no significant difference in immune protection between G-3 and G, nor between SWCNTs-G-3 and SWCNTs-G. These results so far suggest G-3 might be the potential antigen epitope of SVCV. This study lays a foundation for developing vaccine and immunodiagnostic techniques.


Assuntos
Carpas/imunologia , Imunogenicidade da Vacina/imunologia , Nanotubos de Carbono/análise , Rhabdoviridae/imunologia , Animais , Epitopos/imunologia
20.
J Investig Med ; 67(8): 1103-1109, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31575668

RESUMO

Although significant improvements have been achieved in the renal replacement therapy of acute kidney injury (AKI), the mortality of patients with AKI remains high. The aim of this study is to prospectively investigate the capacity of Acute Physiology and Chronic Health Evaluation version II (APACHE II), Simplified Acute Physiology Score version II (SAPS II), Sepsis-related Organ Failure Assessment (SOFA) and Acute Tubular Necrosis Individual Severity Index (ATN-ISI) to predict in-hospital mortality of critically ill patients with AKI. A prospective observational study was conducted in a university teaching hospital. 189 consecutive critically ill patients with AKI were selected according Risk, Injury, Failure, Loss, or End-stage kidney disease criteria. APACHE II, SAPS II, SOFA and ATN-ISI counts were obtained within the first 24 hours following admission. Receiver operating characteristic analyses (ROCs) were applied. Area under the ROC curve (AUC) was calculated. Sensitivity and specificity of in-hospital mortality prediction were calculated. In this study, the in-hospital mortality of critically ill patients with AKI was 37.04% (70/189). AUC of APACHE II, SAPS II, SOFA and ATN-ISI was 0.903 (95% CI 0.856 to 0.950), 0.893 (95% CI 0.847 to 0.940), 0.908 (95% CI 0.866 to 0.950) and 0.889 (95% CI 0.841 to 0.937) and sensitivity was 90.76%, 89.92%, 90.76% and 89.08% and specificity was 77.14%, 70.00%, 71.43% and 71.43%, respectively. In this study, it was found APACHE II, SAPS II, SOFA and ATN-ISI are reliable in-hospital mortality predictors of critically ill patients with AKI. Trial registration number: NCT00953992.

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