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Chem Biol Interact ; 311: 108798, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31433962


Natural products are a valuable source of anticancer agents, with many naturally derived compounds currently used in clinical and preclinical treatments. This study aims to investigate the antiproliferative activity and potential mechanism of action of the xanthoquinodin JBIR-99, isolated from fungi Parengyodontium album MEXU 30,054 and identified by single-crystal X-ray crystallography. Cytotoxicity of xanthoquinodin was evaluated in a panel of human cancer cells lines and CCD-112-CoN normal colon cells, using the sulforhodamine B assay. PC-3 prostate cancer cells were used in biochemical assays including cell cycle, mitochondrial transmembrane potential (MTP), reactive oxygen species (ROS) and caspase activity. Expression levels of apoptosis-pathway-related proteins were analyzed by Western blot. The in vivo toxicity of xanthoquinodin was determined using a zebrafish model. Xanthoquinodin showed cytotoxicity in all cancer cell lines but demonstrated relative selective potency against PC-3 cells with an IC50 1.7 µM. In CCD-112-CoN cells, xanthoquinodin was non-cytotoxic at 100 µM. In PC-3 cells, the compound induced loss of MTP, production of ROS, and cell cycle arrest in S phase. The expression and activity of caspase-3 was increased, which correlates with the upregulation of Cyt c, Bax, nuclear factor kappa-B (NF-κB) (p65) and IKKß, and downregulation of poly ADP ribose polymerase (PARP-1) and Bcl-2. Lastly, xanthoquinodin did not cause any visible developmental toxicity in zebrafish at 50 µM. These results demonstrate xanthoquinodin induces apoptosis in PC-3 prostate cancer cells by activation of both intrinsic and extrinsic apoptotic pathways. In addition, the non-toxic effect in vivo indicates that xanthoquinodin could be a useful lead in the development of a novel, anti-cancer agent that is selective for prostate cancer.

Apoptose/efeitos dos fármacos , Ascomicetos/química , Cromonas/farmacologia , Ascomicetos/metabolismo , Linhagem Celular Tumoral , Cromonas/química , Cristalografia por Raios X , Citocromos c/metabolismo , Humanos , Quinase I-kappa B/metabolismo , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Conformação Molecular , Poli(ADP-Ribose) Polimerase-1/metabolismo , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Proteínas Repressoras/metabolismo , Pontos de Checagem da Fase S do Ciclo Celular/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos
PLoS One ; 14(5): e0216820, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31112581


BACKGROUND AND AIMS: Dronedarone is a new multichannel-blocking antiarrhythmic for the treatment of patients with atrial fibrillation. Our group has demonstrated that dronedarone produces regression of cardiac remodeling; however, its effect on the remodeling of the elastic arteries has not yet been reported. We aim to assess the effects of dronedarone on the regression of thoracic aortic remodeling in spontaneously hypertensive rats (SHRs). METHOD: Ten-month-old male SHRs were randomly assigned to an intervention group (SHR-D), where the animals received dronedarone treatment (100 mg/kg), to a control group (SHR) where rats were given vehicle, or to a group (SHR-A) where they were given amiodarone. A fourth group of normotensive control rats (Wistar-Kyoto rats, WKY) was also added. After two weeks of treatment, we studied the structure, the elastic fiber content of the thoracic aorta using histological techniques and confocal microscopy, and the vascular mechanical properties using an organ bath and isometric tension analysis. A mass spectrometric determination of symmetric dimethylarginine (SDMA) concentrations was performed. RESULTS: SHR group developed the classic remodeling expected from the experimental model: outward hypertrophic remodeling, increased elastic fiber content and wall stiffness. However, the SHR-D group showed statistically significantly lower values for aortic tunica media thickness, wall to lumen ratio, external diameter, cross-sectional area, volume density of the elastic fibers, wall stiffness, and aortic SDMA concentration when compared to the SHR group. These parameters were similar in the SHR and SHR-A groups. Interestingly, the values for tunica media thickness, volume density of the elastic fibers, wall stiffness, and SDMA concentration obtained from the SHR-D group were similar to those measured in the WKY group. CONCLUSION: These results suggest that dronedarone improves the structure and passive mechanical properties of the thoracic aorta in hypertensive rats, and that this protective effect could be associated with a reduction in the concentration of aortic SDMA.

Neuropsychology ; 33(4): 568-580, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30907608


OBJECTIVE: The present study aimed to comprehensively study the specific neurocognitive constructs underlying verbal memory deficits and their neuroanatomical correlates in first episode psychosis (FEP) patients. METHOD: A total of 218 FEP patients and 145 healthy participants were examined with the Rey Auditory Verbal Learning Test (a widely used verbal memory measure that provides a range of performance indexes to evaluate memory) and voxel-based morphometry (a neuroimaging analysis technique that allows investigation of focal differences in brain anatomy). RESULTS: The analyses showed that the FEP group presented significantly lower scores on acquisition/learning, F(1, 566) = 40.7; p < .001, and delayed recall, F(1, 570) = 74.12; p < .001, as well as higher rates of forgetting, F(1, 566) = 20.03; p < .001. They also exhibited a significant sensitivity to retroactive, F(1, 554) = 8.74; p = .003, but not to proactive interference. Neuroimaging analyses found significant interactions between bilateral frontal lobe morphometry and proactive interference (ρFWE = 0.023). Rate of forgetting also significantly interacted with right occipital cortex morphometry (ρFWE = 0.033). Patients with higher rates of forgetting, proactive and retroactive interference demonstrated further gray matter reductions in frontal and occipital cortical areas. CONCLUSIONS: These findings emphasize the anterior orbitofrontal cortex as the brain region that contributes to verbal memory deficits in FEP patients, and suggest specific relationships between different neuroanatomical structures and discrete verbal memory processes. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Encéfalo/diagnóstico por imagem , Memória/fisiologia , Transtornos Psicóticos/psicologia , Adulto , Feminino , Humanos , Imagem por Ressonância Magnética/métodos , Masculino , Rememoração Mental/fisiologia , Testes Neuropsicológicos , Transtornos Psicóticos/diagnóstico por imagem , Adulto Jovem
Nutr Hosp ; 36(1): 80-86, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30836761


Introduction: Background: the aim of this study was to evaluate the intake of nutrients, anthropometric parameters, health indicators, adipokines and insulin levels in a population of young undergraduates. Method: in this study, 378 young undergraduates were invited to participate. Due to the inclusion criteria and their own decision of participating, 90 attended the anthropometric, health indicators: waist circumference (WC), waist to hip ratio (WHR), waist to height ratio (WHtR), and homeostatic model assessment-insulin resistance index (HOMA-IR) studies and completed the questionnaire of frequency of food intake; and 34 participants were selected to perform the determination of biochemical parameters, insulin and adipokines levels: leptin, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and hepatocyte growth factor (HGF). Results: according to WC, WHR and WHtR, obese population showed health, cardiovascular and metabolic risk. Overweight population showed cardiometabolic risk. In general, lipid intake was higher than 30%, being animal fat the most consumed. The levels of leptin (women: 17.2 ± 9.2, 28 ± 11.3, 36.8 ± 17.8; men: 4.3 ± 3.6, 9.5 ± 3.1, 24.6 ± 16.4 to lean overweight and obese, respectively) and insulin (women: 408 ± 182, 438 ± 187, 768 ± 167; men: 244 ± 88, 520 ± 256, 853 ± 590) increased along with body mass index (BMI), body fat percentage (BFP), visceral fat area (VFA), WC, WHR and WHtR. Lean (2.4 ± 1.3), overweight (2.2 ± 0.9) and obese (4.3 ± 1.1) women and overweight (2.8 ± 1.2) and obese (5.0 ± 3.1) men showed insulin resistance according to HOMA-IR. Significant correlation between leptin and HOMA-IR was found (p = 0.41). BMI, BFP, VFA, WC, and WHtR positively correlated with leptin (p = 0.67, 0.75, 0.66, 0.60, 0.67, respectively) and insulin (p = 0.37, 0.40, 0.48, 0.49, 0.42, respectively), while WHR only with insulin (p = 0.43). No significant differences were found in the other adipokines. Conclusion: the use of health indicators such VFA, WC, WHR, WHtR and HOMA-IR are useful tools in the determination of health, cardio vascular and metabolic risk and are correlated with levels of leptin and insulin in the studied population.

Adipocinas/sangue , Antropometria , Dieta , Adiposidade , Composição Corporal , Índice de Massa Corporal , Estudos Transversais , Comportamento Alimentar , Feminino , Humanos , Resistência à Insulina , Leptina/sangue , Masculino , Obesidade/sangue , Sobrepeso/sangue , Fatores Sexuais , Estudantes , Circunferência da Cintura , Relação Cintura-Quadril , Adulto Jovem
Nutr. hosp ; 36(1): 80-86, ene.-feb. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: ibc-FGT-1754


Background: obesity is the excessive accumulation of adipose tissue related to food intake and other factors. The aim of this study was to evaluate the intake of nutrients, anthropometric parameters, health indicators, adipokines and insulin levels in a population of young undergraduates. Method: in this study, 378 young undergraduates were invited to participate. Due to the inclusion criteria and their own decision of participating, 90 attended the anthropometric, health indicators: waist circumference (WC), waist to hip ratio (WHR), waist to height ratio (WHtR), and homeostatic model assessment-insulin resistance index (HOMA-IR) studies and completed the questionnaire of frequency of food intake; and 34 participants were selected to perform the determination of biochemical parameters, insulin and adipokines levels: leptin, IL-6, IL-8, tumor necrosis factor alpha (TNF- α), monocyte chemoattractant protein-1 (MCP-1) and hepatocyte growth factor (HGF). Results: according to WC, WHR and WHtR (women: 104 ± 20, 0.87 ± 0.08, 0.6 ± 0.13; men: 112 ± 10, 0.95 ± 0.09, 0.64 ± 0.06, respectively), obese population showed health, cardiovascular and metabolic risk. Overweight population showed cardiometabolic risk. In general, lipid intake was higher than 30%, being animal fat the most consumed. The levels of leptin (women: 17.2 ± 9.2, 28 ± 11.3, 36.8 ± 17.8; men: 4.3 ± 3.6, 9.5 ± 3.1, 24.6 ± 16.4 to lean overweight and obese, respectively) and insulin (women: 408 ± 182, 438 ± 187, 768 ± 167; men: 244 ± 88, 520 ± 256, 853 ± 590) increased along with body mass index (BMI), body fat percentage (BFP), visceral fat area (VFA), WC, WHR and WHtR. Lean (2.4 ± 1.3), overweight (2.2 ± 0.9) and obese (4.3 ± 1.1) women and overweight (2.8 ± 1.2) and obese (5.0 ± 3.1) men showed insulin resistance according to HOMA-IR. Significant correlation between leptin and HOMA-IR was found (p = 0.41). BMI, BFP, VFA, WC, and WHtR positively correlated with leptin (p = 0.67, 0.75, 0.66, 0.60, 0.67, respectively) and insulin (p = 0.37, 0.40, 0.48, 0.49, 0.42, respectively), while WHR only with insulin (p = 0.43). No significant differences were found in the other adipokines. Conclusion: the use of health indicators such VFA, WC, WHR, WHtR and HOMA-IR are useful tools in the determination of health, cardio vascular and metabolic risk and are correlated with levels of leptin and insulin in the studied population

Introducción: la obesidad es la acumulación excesiva de tejido adiposo relacionada con la ingesta de alimentos y otros factores. El objetivo de este estudio fue evaluar la ingesta de nutrientes, parámetros antropométricos, indicadores de salud, adipocinas y niveles de insulina en una población de jóvenes universitarios con una dieta habitual. Método: en este estudio se invitó a participar a 378 jóvenes universitarios. Debido a los criterios de inclusión y su propia decisión de participar, 90 asistieron a los estudios antropométricos y de indicadores de salud: circunferencia de cintura (WC), índice de cadera cintura (WHR), índice de cintura-talla (WHtR) y modelo homeostático de evaluación-índice de resistencia a la insulina (HOMA-IR) y completaron el cuestionario de frecuencia de ingesta de alimentos. Treinta y cuatro participantes fueron seleccionados para realizar la determinación de los parámetros bioquímicos, niveles de insulina y adipocinas (leptina, IL-6, IL-8, factor de necrosis tumoral alfa [TNF- α], proteína quimioatractante de monocitos-1 [MCP-1] y factor de crecimiento hepático [HGF]). Resultados: de acuerdo con WC, WHR y WHtR (mujeres: 104 ± 20, 0,87 ± 0,08, 0,6 ± 0,13; hombres: 112 ± 10, 0,95 ± 0,09, 0,64 ± 0,06, respectivamente), la población obesa mostró riesgo cardiovascular, metabólico y para la salud. La población con sobrepeso mostró riesgo cardiometabólico. En general, la ingesta de lípidos fue superior al 30% y la grasa animal fue la más consumida. Los niveles de leptina (mujeres: 17,2 ± 9,2, 28 ± 11,3, 36,8 ± 17,8; hombres: 4,3 ± 3,6, 9,5 ± 3,1, 24,6 ± 16,4 para delgados, sobrepeso y obesos, respectivamente) e insulina (mujeres: 408 ± 182, 438 ± 187, 768 ± 167; hombres: 244 ± 88, 520 ± 256, 853 ± 590) aumentaron junto con el índice de masa corporal (BMI), porcentaje de grasa corporal (BFP), área de grasa visceral (VFA), WC, WHR y WHtR. Las mujeres delgadas (2,4 ± 1,3), con sobrepeso (2,2 ± 0,9) y obesas (4,3 ± 1,1) y los hombres con sobrepeso (2,8 ± 1,2) y obesos (5,0 ± 3,1) mostraron resistencia a la insulina de acuerdo con HOMA-IR. Se encontró una correlación significativa entre leptina y HOMA-IR (p = 0,41). BMI, BFP, VFA, WC y WHtR correlacionaron positivamente con leptina (p = 0,67, 0,75, 0,66, 0,60 y 0,67, respectivamente) e insulina (p = 0,37, 0,40, 0,48, 0,49 y 0,42, respectivamente), mientras que el WHR solo con insulina (p = 0,43). No se encontraron diferencias signifi cativas en las otras adipocinas. Conclusión: el uso de indicadores de salud como VFA, WC, WHR, WHtR y HOMA-IR es una herramienta útil en la determinación del riesgo metabólico, cardiovascular y de salud, y dichos indicadores correlacionaron con los niveles de leptina e insulina en la población estudiada

Rev. cient. (Guatem.) ; 28(1): [32]-[43], 20181107.
Artigo em Espanhol | LILACS-Express | ID: biblio-963803


Neolentinus ponderosus y N. lepideus son dos especies de hongos saprobios que poseen uso alimenticio tradicional en comunidades de los departamentos de Huehuetenango y Totonicapán, Guatemala. La capacidad degradadora de ambas especies les confiere potencial para el estudio de la producción de cuerpos fructíferos a nivel de sustrato, por lo que en esta investigación se evaluó el crecimiento de una cepa nativa de N. ponderosus y una de N. lepideus en el medio de cultivo agar extracto de malta (EMA) a diferente pH y también se estudió la degradación de la madera de dos especies de pino (Pinus tecunumanii y P. ayacahuite) en cámaras de podredumbre durante 12 meses. El mayor diámetro de crecimiento miceliar de la cepa N. ponderosus se observó a pH 7.0, en tanto que para N. lepideus se obtuvo a pH 5.0 y 5.6. Las colonias de ambas cepas evidenciaron textura afelpada, borde regular a irregular, color blanco, con o sin pigmento difusible, olor frutal, hifas de 1.0-5.0 µm de diámetro, con clamidosporas y fíbulas. La madera de P. tecunumanii degradada por la cepa de N. ponderosus y la madera de P. ayacahuite por la cepa N. lepideus, presentaron porcentajes de pérdida de peso de 8.76 (±5.58) y 12.07 (±5.66), respectivamente y en ambos casos se alcanzó solamente el estadio temprano de degradación. Los resultados de este estudio sientan las bases para investigaciones posteriores en los que se evalúe el cultivo en troncos de ambas especies para la producción de cuerpos fructíferos con fines alimenticios y comerciales

Neolentinus ponderosus and N. lepideus are two saprophytic fungi species used traditionally in Huehuetenango and Totonicapán, Guatemala. The degradative capacity of both species confers them potential for fruiting bodies production. This study evaluated the mycelial growth of two native strains of N. ponderosus and N. lepideus in malt extract agar (EMA) at different pH and the degradation of wood from two pine species in rot chambers during 12 months. pH 7.0 was the most appropriate for the mycelial growth of N. ponderosus and for N. lepideus were 5.0 and 5.6. The colonies of both strains showed fruity odor, velvety texture, regular to irregular edge, white color, with or without diffusible pigment, hyphae with 1-5 µm width, chlamydospores and clamp connections. Wood from Pinus tecunumanii and P. ayacahuite exhibit weight-loss percentages between 8.76 ± 5.58 and 12.07 ± 5.66, with N. ponderosus 145.2003 and N. lepideus 90.2002, respectively. In both cases reached the early stage of brown-rot decay. These results could be useful for future research that evaluate the fruiting bodies production in logs for food and commercial purposes

Molecules ; 23(9)2018 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-30223456


Anthocyanins in red grape musts may evolve during the winemaking process and wine aging for several different reasons; colour stability and evolution is a complex process that may depend on grape variety, winemaking technology, fermentative yeast selection, co-pigmentation phenomena and polymerization. The condensation of flavanols with anthocyanins may occur either with the flavylium ion or with the hemiacetal formation in order to produce oligomers and polymers. The kinetics of the reaction are enhanced by the presence of metabolic acetaldehyde, promoting the formation of pyranoanthocyanin-type dimers or flavanol-ethyl-anthocyanin structures. The experimental design carried out using white must corrected with the addition of malvidin-3-O-glucoside and flavanols, suggests that non-Saccharomyces yeasts are able to provide increased levels of colour intensity and larger polymeric pigment ratios and polymerization indexes. The selection of non-Saccharomyces genera, in particular Lachancea thermotolerans and Schizosaccharomyces pombe in sequential fermentation, have provided experimental wines with increased fruity esters, as well as producing wines with potential pigment compositions, even though there is an important reduction of total anthocyanins.

Rev. cient. (Guatem.) ; 27(2): [33]-[47], 20180630.
Artigo em Espanhol | LILACS-Express | ID: biblio-906115


Pinus caribaea var. hondurensis (Sénéc.) W. H. Barrett & Golfari es una especie mesoamericana cuyo hábitat natural en Guatemala se está reduciendo aceleradamente por deforestación, aumento de ganadería y producción de cultivos de subsistencia. Además, los hongos ectomicorrícicos asociados, fundamentales para su supervivencia, han sido muy poco estudiados. Este es un estudio descriptivo que contiene información acerca de la diversidad de hongos micorrícicos recolectados en rodales de P. caribaea del municipio de Poptún, Petén, durante los años 1997-1998 y 2013- 2016, del uso de algunos de esos hongos como inóculo micorrícico, de identificación molecular de dos especies de Lactarius y de importantes aspectos etnomicológicos locales. Se encontró que existen especies endémicas, como Boletus guatemalensis, predominancia de boletales y russulales, diversidad fúngica semejante a la del sureste del país, poco uso de hongos comestibles silvestres y el primer registro de Amanita persicina (anteriormente A. muscaria var. persicina) en las tierras bajas mayas de Guatemala, con importantes aportes etnomicológicos. Aunque la diversidad debe ser mucho mayor a la encontrada, es aconsejable proseguir las recolectas, la identificación taxonómica de las especies locales y promover la conservación y reforestación con esta singular especie de pino a nivel municipal

Pinus caribaea var. hondurensis (Sénéc.) W. H. Barrett & Golfari is a Mesoamerican species whose natural habitat in Guatemala is being quickly reduced by deforestation, increase of livestock and subsistence crops. The mycorrhizal fungi associated with them, fundamental for their survival, had not been studied before. In this study it is presented a short report of the ectomycorrhizal fungi diversity collected in the stands of P. caribaea in the village of Poptún, Petén, between the years 1997-1998 and 2013-2016. Endemic species, such as Boletus guatemalensis, and predominance of boletales and russulales, were found. The fungal diversity presents more similarities to the one found in the southeast of the country, compared to the diversity of the west highlands. The first record of Amanita persicina (previously A. muscaria var. persicina) in the Mayan lowlands of Guatemala is reported, as well as related important ethnomycological findings. Nevertheless, it is expected higher diversity in the area, therefore, further research is recommended, regarding the taxonomic identification of the different species and the conservation and reforestation of the area with this pine species

J Physiol ; 596(23): 5791-5806, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29277911


KEY POINTS: Intrauterine growth restriction (IUGR), induced by maternal undernutrition, leads to impaired aortic development. This is followed by hypertrophic remodelling associated with accelerated growth during lactation. Fetal nutrient restriction is associated with increased aortic compliance at birth and at weaning, but not in adult animals. This mechanical alteration may be related to a decreased perinatal collagen deposition. Aortic elastin scaffolds purified from young male and female IUGR animals also exhibit increased compliance, only maintained in adult IUGR females. These mechanical alterations may be related to differences in elastin deposition and remodelling. Fetal undernutrition induces similar aortic structural and mechanical alterations in young male and female rats. Our data argue against an early mechanical cause for the sex differences in hypertension development induced by maternal undernutrition. However, the larger compliance of elastin in adult IUGR females may contribute to the maintenance of a normal blood pressure level. ABSTRACT: Fetal undernutrition programmes hypertension development, males being more susceptible. Deficient fetal elastogenesis and vascular growth is a possible mechanism. We investigated the role of aortic mechanical alterations in a rat model of hypertension programming, evaluating changes at birth, weaning and adulthood. Dams were fed ad libitum (Control) or 50% of control intake during the second half of gestation (maternal undernutrition, MUN). Offspring aged 3 days, 21 days and 6 months were studied. Blood pressure was evaluated in vivo. In the thoracic aorta we assessed gross structure, mechanical properties (intact and purified elastin), collagen and elastin content and internal elastic lamina (IEL) organization. Only adult MUN males developed hypertension (systolic blood pressure: MUNmales  = 176.6 ± 5.6 mmHg; Controlmales  = 136.1 ± 4.9 mmHg). At birth MUN rats were lighter, with smaller aortic cross-sectional area (MUNmales  = (1.51 ± 0.08) × 105  µm2 , Controlmales  = (2.8 ± 0.04) × 105  µm2 ); during lactation MUN males and females exhibited catch-up growth and aortic hypertrophy (MUNmales  = (14.5 ± 0.5) × 105  µm2 , Controlmales  = (10.4 ± 0.9) × 105  µm2 ), maintained until adulthood. MUN aortas were more compliant until weaning (functional stiffness: MUNmales  = 1.0 ± 0.04; Controlmales  = 1.3 ± 0.03), containing less collagen with larger IEL fenestrae, returning to normal in adulthood. Purified elastin from young MUN offspring was more compliant in both sexes; only MUN adult females maintained larger elastin compliance (slope: MUNfemales  = 24.1 ± 1.9; Controlfemales  = 33.3 ± 2.8). Fetal undernutrition induces deficient aortic development followed by hypertrophic remodelling and larger aortic compliance in the perinatal period, with similar alterations in collagen and elastin in both sexes. The observed alterations argue against an initial mechanical cause for sex differences in hypertension development. However, the maintenance of high elastin compliance in adult females might protect them against blood pressure rise.

Food Chem ; 239: 975-983, 2018 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-28873660


Non-Saccharomyces yeasts may contribute to enrich wine aroma while promoting the formation of stable pigments. Yeast metabolites such as acetaldehyde and pyruvate participate in the formation of stable pigments during fermentation and wine aging. This work evaluated the formation of polymeric pigments in red musts added with (+)-Catechin, ProcyanidinB2 and ProcyanidinC1. The non-Saccharomyces yeasts used were Lachancea thermotolerans, Metschnikowia pulcherrima and Torulaspora delbrueckii in sequential fermentation with Saccharomyces cerevisiae and Schizosaccharomyces pombe. Use of Lachancea thermotolerans led to larger amounts of polymeric pigments in sequential fermentation. (+)-Catechin is the flavanol prone to forming such pigments. The species Metschnikowia pulcherrima produced higher concentration of esters and total volatile compounds. The sensory analysis pointed out differences in fruitiness and aroma quality. The results obtained strengthen the fact that metabolites from non-Saccharomyces yeasts may contribute to form stable polymeric pigments while also influencing wine complexity.

Vinho , Catequina , Fermentação , Polifenóis , Vitis , Leveduras
J Nat Prod ; 80(8): 2311-2318, 2017 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-28796494


Two new dioxomorpholines, 1 and 2, three new derivatives, 3-5, and the known compound PF1233 B (6) were isolated from a marine-facultative Aspergillus sp. MEXU 27854. Their structures were established by 1D and 2D NMR and HRESIMS data analysis. The absolute configuration of 1 and 2 was elucidated by comparison of experimental and DFT-calculated vibrational circular dichroism spectra. Compounds 3, 5, and 6 were noncytotoxic to a panel of human cancer cell lines with different functional status for the tumor-suppressor protein p53, but were inhibitors of P-glycoprotein-reversing multidrug resistance in a doxorubicin-resistant cell line.

Subfamília B de Transportador de Cassetes de Ligação de ATP/agonistas , Subfamília B de Transportador de Cassetes de Ligação de ATP/química , Aspergillus/química , Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo , Linhagem Celular Tumoral , Dicroísmo Circular , Resistência a Múltiplos Medicamentos , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular
Ter. psicol ; 35(1): 5-14, Apr. 2017. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-846327


The aim of this study was to examine the psychometric properties of the solution-Focused Inventory (SFI) in a Spanish population, replicating the original validation study of Grant et al. (2012). The SFI, designed to evaluate solution-focused thinking, is a 12-item scale with three subscales: problem Disengagement, goal orientation and resource activation. The instrument was adapted and translated into Spanish, then two studies were carried out to examine its psychometric properties. The first study confirmed the instrument's structure, and its good internal consistency. The second study confirmed its convergent validity; SFI was positively correlated with measures of well-being, resilience, satisfaction with life and perspective taking, and negatively correlated with psychopathology. This second study confirmed the stability of the SFI scores across time. In sum, these two studies provide additional support for the reliability and validity of the SFI as a measure of solution-focused thinking, and open its use to Spanish-speaking populations.

El objetivo de este estudio fue analizar las propiedades psicométricas del cuestionario centrado en soluciones (CCS) en una muestra española, replicando el estudio original de Grant et al. (2012). El CCS diseñado para evaluar los pensamientos centrados en las soluciones, compuesta de tres subescalas: Distanciamiento del problema, orientación a la Meta y activación de recursos. Fue traducido y adaptado al castellano, posteriormente se realizaron dos estudios para examinar sus propiedades psicométricas. El primer estudio confirmó la estructura del instrumento y obtuvo una buena consistencia interna. El segundo estudio confirmó su validez convergente; el CCS correlacionó positivamente con el bienestar, resiliencia, satisfacción con la vida y toma de perspectiva, y negativamente con una medida de psicopatología. El segundo estudio confirmó la estabilidad, a lo largo del tiempo. Estos dos estudios confirman que el CCS es una medida que evalúa pensamientos centrados en las soluciones y puede ser utilizado para población española.

Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Resolução de Problemas , Inquéritos e Questionários , Análise Fatorial , Metas , Felicidade , Satisfação Pessoal , Psicometria
Rev. cient. (Guatem.) ; 26(2): [49]-[57], octubre. 2016.
Artigo em Espanhol | LILACS-Express | ID: biblio-883274


Los hongos conidiales se desarrollan en ambientes muy variados en los que influye la temperatura, la humedad y la profundidad de la hojarasca. En esta investigación se analizó la influencia que tiene la humedad del ambiente, microambiente y hojarasca; la temperatura del ambiente y microambiente y la profundidad de la hojarasca, en el desarrollo de 14 especies de hongos anamórficos encontrados en la Reserva Ecológica Cayalá, un bosque urbano ubicado en la Ciudad de Guatemala. Este análisis se realizó por medio de muestreos durante la época lluviosa de julio a noviembre de 2013 y por estadística multivariada para asociar las distintas variables con las especies fúngicas. Se encontró que la presencia de Bactrodesmium longisporum MB Ellis, Beltrania rhombica Penz, Michelia, Junewangia globulosa (Thoth) WA Baker & Morgan - Jones y Neopodocodis megasperma (Boedijin) Rifai fueron influenciadas por la profundidad de la hojarasca y la temperatura del microambiente mientras que el resto de especies lo fueron por la humedad del ambiente, microambiente y hojarasca. Es importante conocer la forma en que las variables evaluadas afectan la presencia de estos hongos, por su acción en la dinámica de remanentes boscosos urbanos, para contribuir a su conservación

Conidial fungi develop in distinct habitats with several environmental conditions in which influences the temperature, humidity and depth of litter. In this research the influence of the humidity, temperature, microenvironment and litter moisture and temperature and depth of litter, was analyzed in the development of 14 species of anamorphic fungi found in the Reserva Ecológica Cayalá, located in Guatemala City. This analysis was performed by sampling during the rainy season from July to November 2013 and using multivariate statistics for different variables associated with the species. It was found that the presence of Bactrodesmium longisporum MB Ellis, Beltrania rhombica Penz, Michelia, Junewangia globulosa (Thoth) WA Baker & Morgan - Jones, and Neopodocodis megasperma(Boedijin) Rifai, was influenced by the depth of litter and microenvironment temperature while the other species were for the environmental humidity, microenvironment moisture and litter moisture. It is important to know how the evaluated variables affect the presence of these fungi, by the roleits action on the dynamics of urban forest remmants, to contribute to their conservation.

Rev. cient. (Guatem.) ; 26(1): [40]-[50], octubre 2016.
Artigo em Espanhol | LILACS-Express | ID: biblio-883303


Los hongos anamorfos constituyen un grupo muy diverso microscópicos cuya forma de reproducción es asexual a través de conidios. Actúan como agentes de degradación de la materia vegetal en el suelo, lo cual es un proceso clave en el reciclaje de nutrientes. Debido a la importancia y al vacío de información que existe sobre estos hongos en Guatemala, en este trabajo se identificaron 12 especies, por medio de características microscópicas; luego de la extracción de las estructuras fúngicas desarrolladas en hojas, ramas y semillas recolectadas en una parcela de la Reserva Ecológica Cayalá de la Ciudad de Guatemala. El material de referencia está depositado en la colección de hongos anamorfos de la Micoteca de Macrohongos de Guatemala "Lic. Rubén Mayorga Peralta" (MICG)

Anamorphic fungi are a diverse group of microscopic fungi that reproduce asexually through conidia. They degrade plant debris on the soil, which is a key process in nutrients recycling. Because of its importance and the lack of information regarding to these fungi in Guatemala, In this article, 12 species were identified through microscopic characteristics, after extraction of fungal structures developed in leaves, twigs and seeds collected at the Cayalá Ecological Reserve in Guatemala City. Reference materials are placed at the anamorphic fungi collection at Micoteca de Macro hongos de Guatemala, "Dr. Ruben Mayorga Peralta" (MICG).

Rev Med Inst Mex Seguro Soc ; 53 Suppl 3: S240-5, 2015.
Artigo em Espanhol | MEDLINE | ID: mdl-26509299


BACKGROUND: Survival of children with pineal region tumors has increased significantly in the last decade; these tumors have an insidious outcome associated with endocrine disorders with high morbidity and mortality, especially after gross resection. The objective was to report the survival, outcome, morbidity and mortality according to type of surgery, histology and treatment in children with pineal region tumors. METHODS: This retrospective study included all patients of 17 years or less with diagnosis of pineal region tumor, who went over a period of 10 years to a children's hospital. A histopathological review was made, and the extent of resection was determined. The survival was also estimated. RESULTS: Forty-six patients were included, out of which 36 had complete medical records and adequate pathologic material. Gross resection was performed in 24 (66.6 %), and biopsy in 12 (33.3 %); 23 (88 %) patients died; hydroelectrolytic imbalance was the cause of 14 deaths (60 %) and the other nine (39.1 %) were secondary to tumor progression. Ten-years survivals among patients treated with gross resection and biopsy were 52 and 75 %, respectively (p = 0.7). Endocrine alterations were observed in 13 patients (36.1 %); in 10 of these (76.9 %) the total resection was performed. CONCLUSIONS: Pineal region tumors in children can be treated with diagnostic biopsy, followed by adjuvant treatment consisting of chemotherapy and radiotherapy.

Astrocitoma/cirurgia , Neoplasias Encefálicas/cirurgia , Germinoma/cirurgia , Tumores Neuroendócrinos/cirurgia , Glândula Pineal/cirurgia , Adolescente , Astrocitoma/mortalidade , Astrocitoma/patologia , Astrocitoma/terapia , Neoplasias Encefálicas/mortalidade , Neoplasias Encefálicas/patologia , Neoplasias Encefálicas/terapia , Quimiorradioterapia Adjuvante , Criança , Pré-Escolar , Feminino , Seguimentos , Germinoma/mortalidade , Germinoma/patologia , Germinoma/terapia , Humanos , Lactente , Masculino , Tumores Neuroendócrinos/mortalidade , Tumores Neuroendócrinos/patologia , Tumores Neuroendócrinos/terapia , Glândula Pineal/patologia , Estudos Retrospectivos , Análise de Sobrevida , Resultado do Tratamento
J Nutr Biochem ; 26(12): 1650-9, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26350253


Intrauterine growth retardation predisposes to hypertension development, known as fetal programming. Females are less susceptible, which has been mainly attributed to estrogen influence. We hypothesize that perinatal differences in oxidative status might also contribute. We studied 21-day-old (prepuberal) and 6-month-old male and female offspring from rats fed ad libitum during gestation (Control) or with 50% of Control daily intake from day 10 to delivery (maternal undernutrition, MUN). We assessed in vivo blood pressure and the following plasma biomarkers of oxidative status: protein carbonyls, thiols, reduced glutathione (GSH), total antioxidant capacity, superoxide anion scavenging activity (SOSA) and catalase activities; we calculated a global score (oxy-score) from them. Estradiol and melatonin concentration was measured in young rats. Prepuberal MUN males were normotensive but already exhibited increased carbonyls and lower thiols, GSH, SOSA and melatonin; oxy-score was significantly lower compared to Control males. Prepuberal MUN females only exhibited reduced SOSA compared to Control females. Adult rats from all experimental groups showed a significant increase in carbonyls and a decrease in antioxidants compared to prepuberal rats; oxy-score was negative in adult rats suggesting the development of a prooxidative status as rat age. Adult MUN males were hypertensive and exhibited the highest increase in carbonyls despite similar or even higher antioxidant levels compared to Controls. Adult MUN females remained normotensive and did not exhibit differences in any of the biomarkers compared to Controls. The better global antioxidant status developed by MUN females during perinatal life could contribute to their protection against hypertension programming.

Antioxidantes/metabolismo , Transtornos da Nutrição Fetal/sangue , Oxigênio/sangue , Fatores Sexuais , Animais , Animais Recém-Nascidos , Biomarcadores/sangue , Pressão Sanguínea , Peso Corporal , Catalase/metabolismo , Modelos Animais de Doenças , Estradiol/sangue , Estrogênios/sangue , Feminino , Retardo do Crescimento Fetal/sangue , Glutationa/sangue , Masculino , Melatonina/sangue , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley , Compostos de Sulfidrila/sangue
J Nat Prod ; 78(4): 730-5, 2015 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-25706243


Two new compounds, pestalotin 4'-O-methyl-ß-mannopyranoside (1) and 3S,4R-(+)-4-hydroxymellein (2), were isolated from an organic extract of a Xylaria feejeensis, which was isolated as an endophytic fungus from Hintonia latiflora. In addition, the known compounds 3S,4S-(+)-4-hydroxymellein (3), 3S-(+)-8-methoxymellein (4), and the quinone derivatives 2-hydroxy-5-methoxy-3-methylcyclohexa-2,5-diene-1,4-dione (5), 4S,5S,6S-4-hydroxy-3-methoxy-5-methyl-5,6-epoxycyclohex-2-en-1-one (6), and 4R,5R-dihydroxy-3-methoxy-5-methylcyclohexen-2-en-1-one (7) were obtained. The structures of 1 and 2 were elucidated using a set of spectroscopic and spectrometric techniques. The absolute configuration of the stereogenic centers of 1 and 2 was determined using ECD spectroscopy combined with time-dependent density functional theory calculations. In the case of 1, comparison of the experimental and theoretical (3)J6-7 coupling constants provided further evidence for the stereochemical assignments. Compounds 2 and 3 inhibited Saccharomyces cerevisiae α-glucosidase (αGHY), with IC50 values of 441 ± 23 and 549 ± 2.5 µM, respectively. Their activity was comparable to that of acarbose (IC50 = 545 ± 19 µM), used as positive control. Molecular docking predicted that both compounds bind to αGHY in a site different from the catalytic domain, which could imply an allosteric type of inhibition.

Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Inibidores de Glicosídeo Hidrolases/farmacologia , Isocumarinas/isolamento & purificação , Isocumarinas/farmacologia , Manose/análogos & derivados , Rubiaceae/microbiologia , Xylariales/química , alfa-Glucosidases/efeitos dos fármacos , Acarbose/farmacologia , Algoritmos , Inibidores de Glicosídeo Hidrolases/química , Isocumarinas/química , Manose/química , Manose/isolamento & purificação , Manose/farmacologia , México , Estrutura Molecular
Org Biomol Chem ; 13(5): 1429-38, 2015 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-25469759


The three-armed pyrenyl-triazole receptor 1 behaves as a highly selective fluorescent molecular sensor for citrate anions over similar carboxylates such as malate or tartrate. In addition, this receptor senses Cu(2+) cations through absorption and emission channels even in the presence of Hg(2+) metal cations. The related three-armed ferrocenyl-triazole receptor 2 behaves as a highly selective dual (redox and chromogenic) chemosensor molecule for Pb(2+) metal cations.

Acupunct Med ; 32(6): 486-94, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25267728


OBJECTIVE: To investigate the antihyperglycaemic activity of laser acupuncture stimulation at 650 and 980 nm at BL20 in streptozotocin (STZ)-induced diabetic rats. METHODS: Seventy healthy adult male albino Wistar rats weighing 250±50 g were divided into seven groups of 10 animals each. Groups I-III comprised healthy control rats which were untreated (I) or stimulated with laser acupuncture at 650 nm (II) and 980 nm (III), respectively. Groups IV-VII underwent induction of diabetes with a single intraperitoneal administration of STZ at 50 mg/kg. Animals with blood glucose levels of ≥200 mg/dL on the fifth day were used for the experiments and were left untreated (group IV), treated with glibenclamide (group V) or stimulated with laser acupuncture at 650 nm (group VI) and 980 nm (group VII), respectively. Laser acupuncture was applied at BL20 on alternate days for a total of 12 sessions over a 28-day period. RESULTS: After 28 days of treatment, STZ-induced diabetic rats stimulated with laser acupuncture at 650 and 980 nm had significantly lower glucose levels compared with untreated diabetic rats (242.0±65.0 and 129.8±33.2 vs 376.5±10.0 mg/dL, both p≤0.05). Treatment at 980 nm also attenuated the increase in glucose between day 1 and day 28 compared with the glibenclamide-treated diabetic group (41.5±19.6 mg/dL vs 164.1±13.7 g/dL, p<0.05). Laser acupuncture treatment did not affect the blood count or biochemical profile and was not associated with any morphological changes in the pancreas, liver, kidney or spleen. CONCLUSIONS: Stimulation with laser acupuncture at 650 and 980 nm at BL20 in STZ-induced diabetic rats has antihyperglycaemic activity. The results support further evaluation of laser acupuncture as an alternative or complementary treatment for the control of hyperglycaemia.

Terapia por Acupuntura , Glicemia/metabolismo , Diabetes Mellitus Experimental/terapia , Hiperglicemia/terapia , Terapia por Acupuntura/métodos , Animais , Lasers , Masculino , Ratos Wistar
Zookeys ; (399): 43-69, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24843252


We describe the composition and distribution of bivalve molluscs from the sandy and rocky intertidal and the shallow subtidal environments of Bahía de Mazatlán, México. The bivalve fauna of the bay is represented by 89 living species in 28 families, including 37 new records and four range extensions: Lithophaga hastasia, Adula soleniformis, Mactrellona subalata, and Strigilla ervilia. The number of species increases from the upper (44) and lower intertidal (53) to the shallow subtidal (76), but only 11 (17%) have a wide distribution in the bay (i.e., found in all sampling sites and environments). The bivalve assemblages are composed of four main life forms: 27 epifaunal species, 26 infaunal, 16 semi-infaunal, and 20 endolithic. A taxonomic distinctness analysis identified the sampling sites and environments that contribute the most to the taxonomic diversity (species to suborder categories) of the bay. The present work increased significantly (31%) to 132 species previous inventories of bivalves of Bahía de Mazatlán. These species represent 34% of the bivalve diversity of the southern Golfo de California and approximately 15% of the Eastern Tropical Pacific region.