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1.
J Exp Bot ; 2020 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-33367755

RESUMO

Sucrose metabolism is of high importance for most plant species, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. Two cleaving enzymes channel sucrose into sink metabolism; sucrose synthases (SUS) and invertases (INV), which are localized in different subcellular compartments. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a previously reported pathway QTL largely affecting primary carbon metabolism in tomato. Of the eight A/N-INV genes identified in the tomato genome, NI6 mRNA is present in all organs, but its expression was higher in sink tissues (mainly roots and fruits). A NI6-GFP fusion protein was found in the cytosol of mesophyll cells. Tomato NI6-silenced plants showed impaired growth phenotypes, delayed flowering and dramatic reduction in the fruit set. Global gene expression and metabolite profile analyses of these plants revealed that NI6 is not only essential for sugar metabolism but also plays a signaling role in stress adaptation. Gene-metabolite network analyses allowed identification of major hubs, whose expression patterns were greatly affected by NI6 silencing, within the signaling cascade that coordinates carbohydrate metabolism with growth and development in tomato.

2.
Microorganisms ; 8(8)2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32785053

RESUMO

The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress.

3.
Biol Res ; 53(1): 29, 2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32631429

RESUMO

Arid environments are defined by the lack of water availability, which is directly related to the mean annual precipitation (MAP), and high values of solar irradiation, which impacts the community composition of animals, plants, and the microbial structure of the soil. Recent advances in NGS technologies have expanded our ability to characterize microbiomes, allowing environmental microbiologists to explore the complete microbial structure. Intending to identify and describe the state-of-the-art of bacterial communities in arid soils at a global scale, and to address the effect that some environmental features may have on them, we performed a systematic review based on the PRISMA guideline. Using a combination of keywords, we identified a collection of 66 studies, including 327 sampled sites, reporting the arid soil bacterial community composition by 16S rDNA gene high-throughput sequencing. To identify factors that can modulate bacterial communities, we extracted the geographical, environmental, and physicochemical data. The results indicate that even though each sampled site was catalogued as arid, they show wide variability in altitude, mean annual temperature (MAT), soil pH and electric conductivity, within and between arid environments. We show that arid soils display a higher abundance of Actinobacteria and lower abundance of Proteobacteria, Cyanobacteria, and Planctomycetes, compared with non-arid soil microbiomes, revealing that microbial structure seems to be strongly modulated by MAP and MAT and not by pH in arid soils. We observed that environmental and physicochemical features were scarcely described among studies, hence, we propose a reporting guideline for further analysis, which will allow deepening the knowledge of the relationship between the microbiome and abiotic factors in arid soil. Finally, to understand the academic collaborations landscape, we developed an analysis of the author's network, corroborating a low degree of connectivity and collaborations in this research topic. Considering that it is crucial to understand how microbial processes develop and change in arid soils, our analysis emphasizes the need to increase collaborations between research groups worldwide.


Assuntos
Microbiota , Microbiologia do Solo , Animais , Microbiota/genética , Plantas , Proteobactérias , Solo
4.
Microorganisms ; 8(6)2020 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-32599933

RESUMO

Autochthonous microorganisms are an important source of the distinctive metabolites that influence the chemical profile of wine. However, little is known about the diversity of fungal communities associated with grape musts, even though they are the source of local yeast strains with potential capacities to become starters during fermentation. By using internal transcribed spacer (ITS) amplicon sequencing, we identified the taxonomic structure of the yeast community in unfermented and fermented musts of a typical Vitis vinifera L. var. Sauvignon blanc from the Central Valley of Chile throughout two consecutive seasons of production. Unsurprisingly, Saccharomyces represented the most abundant fungal genus in unfermented and fermented musts, mainly due to the contribution of S. uvarum (42.7%) and S. cerevisiae (80%). Unfermented musts were highly variable between seasons and showed higher values of fungal diversity than fermented musts. Since microbial physiological characterization is primarily achieved in culture, we isolated nine species belonging to six genera of fungi from the unfermented must samples. All isolates were characterized for their potential capacities to be used as new starters in wine. Remarkably, only Metschnikowia pulcherrima could co-exist with a commercial Saccharomyces cerevisiae strain under fermentative conditions, representing a feasible candidate strain for wine production.

5.
J Intensive Care Med ; 35(9): 927-932, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32677498

RESUMO

The coronavirus disease 2019 pandemic resulted in unprecedented numbers of patients with respiratory failure requiring ventilatory support. The number of patients who required critical care quickly outpaced the availability of intensive care unit (ICU) beds. Consequently, health care systems had to creatively expand critical care services into alternative hospital locations with repurposed staff and equipment. Deploying anesthesia workstations to the ICU to serve as mechanical ventilators requires equipment preparation, multidisciplinary planning, and targeted education. We aim to contextualize this process, highlighting major differences between anesthesia workstations and ICU ventilators, and to share the insights gained from our experiences creating an anesthesia provider-based ventilator management team.


Assuntos
Anestesia Geral/instrumentação , Infecções por Coronavirus/terapia , Unidades de Terapia Intensiva/organização & administração , Equipe de Assistência ao Paciente/organização & administração , Pneumonia Viral/terapia , Respiração Artificial/instrumentação , Ventiladores Mecânicos/provisão & distribução , Betacoronavirus , Humanos , Pandemias
7.
Mar Drugs ; 18(5)2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32414006

RESUMO

Pesticides cause severe environmental damage to marine ecosystems. In the last ten years, cypermethrin has been extensively used as an antiparasitic pesticide in the salmon farming industry located in Northern Patagonia. The objective of this study was the biochemical and genomic characterization of cypermethrin-degrading and biosurfactant-producing bacterial strains isolated from cypermethrin-contaminated marine sediment samples collected in southern Chile (MS). Eleven strains were isolated by cypermethrin enrichment culture techniques and were identified by 16S rDNA gene sequencing analyses. The highest growth rate on cypermethrin was observed in four isolates (MS13, MS15a, MS16, and MS19) that also exhibited high levels of biosurfactant production. Genome sequence analyses of these isolates revealed the presence of genes encoding components of bacterial secondary metabolism, and the enzymes esterase, pyrethroid hydrolase, and laccase, which have been associated with different biodegradation pathways of cypermethrin. These novel cypermethrin-degrading and biosurfactant-producing bacterial isolates have a biotechnological potential for biodegradation of cypermethrin-contaminated marine sediments, and their genomes contribute to the understanding of microbial lifestyles in these extreme environments.

8.
Pathogens ; 9(5)2020 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-32397152

RESUMO

Piscirickettsia salmons, the causative agent of piscirickettsiosis, is genetically divided into two genomic groups, named after the reference strains as LF-89-like or EM-90-like. Phenotypic differences have been detected between the P. salmonis genogroups, including antibiotic susceptibilities, host specificities and pathogenicity. In this study, we aimed to develop a rapid, sensitive and cost-effective assay for the differentiation of the P. salmonis genogroups. Using an in silico analysis of the P. salmonis 16S rDNA digestion patterns, we have designed a genogroup-specific assay based on PCR-restriction fragment length polymorphism (RFLP). An experimental validation was carried out by comparing the restriction patterns of 13 P. salmonis strains and 57 field samples obtained from the tissues of dead or moribund fish. When the bacterial composition of a set of field samples, for which we detected mixtures of bacterial DNA, was analyzed by a high-throughput sequencing of the 16S rRNA gene amplicons, a diversity of taxa could be identified, including pathogenic and commensal bacteria. Despite the presence of mixtures of bacterial DNA, the characteristic digestion pattern of the P. salmonis genogroups could be detected in the field samples without the need of a microbiological culture and bacterial isolation.

11.
Rev. chil. cardiol ; 39(1): 43-48, abr. 2020. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1115449

RESUMO

A nivel mundial, la tercera causa de muerte por causa cardiovascular es el Tromboembolismo Pulmonar (TEP), después del Infarto agudo de Miocardio y el Accidente cerebrovascular, con una incidencia anual estimada de 40 casos por cada 100.000 habitantes. Se comunica el caso clínico de un paciente de 44 años con diagnóstico de TEP recurrente con Hipertensión pulmonar tromboembólica crónica (CTPH) que fue sometido a tromboendarterectomía pulmonar bilateral (PTE) bajo paro circulatorio con hipotermia profunda. Se informa de los resultados de los medios de diagnóstico y del tratamiento quirúrgico mediante la tromboendarterectomía. Se discute el tema en el contexto de la experiencia internacional y nacional.


Assuntos
Humanos , Masculino , Adulto , Embolia Pulmonar/cirurgia , Endarterectomia/métodos , Hipertensão Pulmonar/cirurgia , Embolia Pulmonar/diagnóstico , Hipertensão Pulmonar/diagnóstico , Hipotermia Induzida
12.
Food Chem ; 319: 126360, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32151896

RESUMO

Sweet cherry is a valuable non-climacteric fruit with elevated phytonutrients, whose fruit quality attributes are prone to rapid deterioration after harvest, especially peel damage and water loss of stem. Here the metabolic and transcriptional response of exogenous melatonin was assessed in two commercial cultivars of sweet cherry (Santina and Royal Rainier) during cold storage. Gene expression profiling revealed that cuticle composition and water movement may underlie the effect of melatonin in delaying weight loss. An effect of melatonin on total soluble solids and lower respiration rate was observed in both cultivars. Melatonin induces overexpression of genes related to anthocyanin biosynthesis, which correlates with increased anthocyanin levels and changes in skin color (Chroma). Our results indicate that along with modulating antioxidant metabolism, melatonin improves fruit quality traits by triggering a range of metabolic and gene expression changes, which ultimately contribute to extend sweet cherry postharvest storability.


Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Melatonina/farmacologia , Prunus avium/efeitos dos fármacos , Antocianinas/metabolismo , Frutas/metabolismo , Prunus avium/metabolismo
13.
Sci Rep ; 10(1): 5560, 2020 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-32221328

RESUMO

The Atacama Desert is the most arid desert on Earth, focus of important research activities related to microbial biodiversity studies. In this context, metabolic characterization of arid soil bacteria is crucial to understand their survival strategies under extreme environmental stress. We investigated whether strain-specific features of two Microbacterium species were involved in the metabolic ability to tolerate/adapt to local variations within an extreme desert environment. Using an integrative systems biology approach we have carried out construction and comparison of genome-scale metabolic models (GEMs) of two Microbacterium sp., CGR1 and CGR2, previously isolated from physicochemically contrasting soil sites in the Atacama Desert. Despite CGR1 and CGR2 belong to different phylogenetic clades, metabolic pathways and attributes are highly conserved in both strains. However, comparison of the GEMs showed significant differences in the connectivity of specific metabolites related to pH tolerance and CO2 production. The latter is most likely required to handle acidic stress through decarboxylation reactions. We observed greater GEM connectivity within Microbacterium sp. CGR1 compared to CGR2, which is correlated with the capacity of CGR1 to tolerate a wider pH tolerance range. Both metabolic models predict the synthesis of pigment metabolites (ß-carotene), observation validated by HPLC experiments. Our study provides a valuable resource to further investigate global metabolic adaptations of bacterial species to grow in soils with different abiotic factors within an extreme environment.


Assuntos
Actinobacteria/genética , Redes e Vias Metabólicas/genética , Adaptação Fisiológica/genética , Altitude , Proteínas de Bactérias/genética , Biodiversidade , Clima Desértico , Genoma Bacteriano/genética , Concentração de Íons de Hidrogênio , Filogenia , Solo , Microbiologia do Solo
14.
Biol. Res ; 53: 29, 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1124216

RESUMO

Abstract Arid environments are defined by the lack of water availability, which is directly related to the mean annual precipitation (MAP), and high values of solar irradiation, which impacts the community composition of animals, plants, and the microbial structure of the soil. Recent advances in NGS technologies have expanded our ability to characterize micro- biomes, allowing environmental microbiologists to explore the complete microbial structure. Intending to identify and describe the state-of-the-art of bacterial communities in arid soils at a global scale, and to address the effect that some environmental features may have on them, we performed a systematic review based on the PRISMA guideline. Using a combination of keywords, we identified a collection of 66 studies, including 327 sampled sites, reporting the arid soil bacterial community composition by 16S rDNA gene high-throughput sequencing. To identify factors that can modulate bacterial communities, we extracted the geographical, environmental, and physicochemical data. The results indicate that even though each sampled site was catalogued as arid, they show wide variability in altitude, mean annual temperature (MAT), soil pH and electric conductivity, within and between arid environments. We show that arid soils display a higher abundance of Actinobacteria and lower abundance of Proteobacteria, Cyanobacteria, and Planctomycetes, compared with non-arid soil microbiomes, revealing that microbial structure seems to be strongly modulated by MAP and MAT and not by pH in arid soils. We observed that environmental and physicochemical features were scarcely described among studies, hence, we propose a reporting guideline for further analysis, which will allow deepening the knowledge of the relationship between the microbiome and abiotic factors in arid soil. Finally, to understand the academic collaborations landscape, we developed an analysis of the author's network, corroborating a low degree of connectivity and collaborations in this research topic. Considering that it is crucial to understand how microbial processes develop and change in arid soils, our analysis emphasizes the need to increase collaborations between research groups worldwide.


Assuntos
Animais , Microbiologia do Solo , Microbiota/genética , Plantas , Solo , Proteobactérias
15.
Data Brief ; 25: 103972, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31249848

RESUMO

The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.

16.
Rev. colomb. psiquiatr ; 48(2): 96-104, ene.-jun. 2019. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1042853

RESUMO

RESUMEN Objetivo: Colombia está enfrentado una epidemia emergente del consumo endovenoso de heroína. Un conocimiento de los programas existentes que ofrecen tratamiento asistido con metadona en el país es necesario para que se puedan proponer estrategias de mejoría. Métodos: Se encuestaron 13 programas de regiones prioritarias. Se evaluaron las caracte rísticas demográficas y clínicas de los usuarios, así como los servicios ofrecidos por estos programas, sus protocolos de tratamiento con metadona y las diferencias en las barreras al tratamiento y las causas de abandono del tratamiento. Resultados: Se analizaron 12/13 cuestionarios, con un total de 538 pacientes activos. La mayo ría de los pacientes eran varones (85,5%) de 18 a 34 arios (70%). El 40% eran usuarios de drogas intravenosas y el 25% admitió compartir agujas. Entre las comorbilidades asociadas con el consumo de heroína se encontró la enfermedad mental (48%), la hepatitis C (8,7%) y la infección por el VIH (2%). La comorbilidad psiquiátrica se asocia más con los pacientes que acuden al sector privado (el 69,8 frente al 29,7%; p < 0,03). La media de la dosis inicial de metadona es 25,3 ± 8,9mg/día y las dosis de mantenimiento van de 41 a 80 mg/día. La falta de articulación con atención primaria fue una barrera más sentida que los problemas con la cobertura del seguro médico y los prejuicios del tratamiento con metadona (p < 0,05). También, los problemas administrativos y de la aseguradora (p < 0,003), la falta de sumi nistro de metadona (p < 0,018) y la recaída en el consumo (p < 0,014) son las razones más significativas de abandono del tratamiento. Conclusiones: Estos programas tienen diferentes niveles de desarrollo e implementación en los protocolos de tratamiento. Algunas de las barreras de acceso y de las causas de abandono del tratamiento pueden mitigarse mejorando la administración de salud.


ABSTRACT Objective: Colombia is facing a rising epidemic of intravenous heroin use. Knowledge of the methadone-assisted treatment programs in the country is crucial in order to propose improvement strategies. Methods: 13 programmes from priority regions were surveyed. The demographic and clinical characteristics of the patients attending the programs, a description of the services offered, their methadone treatment protocols, the various barriers to treatment and the causes of treatment abandonment were reviewed. Results: 12/13 questionnaires were analysed with a total of 538 active patients. Most of the patients attending these programs were men (85.5%) between 18 and 34 years-old (70%). Forty percent (40%) were intravenous drug users and 25% admitted sharing needles. The comorbidities associated with heroin use were mental illness (48%), hepatitis C (8.7%) and HIV (2%). Psychiatric comorbidity was more likely in patients attending the private sec tor (69.8% vs 29.7%; p < 0.03). The initial average dose of methadone administered was 25.3 ± 8.9mg/day, with a maintenance dose ranging from 41 to 80mg/day. Lack of align ment with primary care was perceived to be the most serious barrier to access, ahead of problems with insurance and prejudice towards treatment with methadone (p < 0.05). Health Administration and insurance problems (p < 0.003), together with the lack of availa bility of methadone (p < 0.018) and relapse (p < 0.014) were the most important reasons for abandonment of treatment. Conclusions: The treatment protocols of these programmes offer different levels of develop ment and implementation. Some of the barriers to access and reasons for abandonment of treatment with methadone can be mitigated with better health administration.

17.
Interacciones ; 5(2): 8, 01 de mayo de 2019.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1049643

RESUMO

El presente trabajo contó con el propósito de señalar y, en la medida de lo posible, resolver algunos de los problemas conceptuales relacionados con el área de conocimiento que comúnmente se denomina desarrollo psicológico. El primer paso para lograr esto, consistió en clarificar si el desarrollo psicológico es una subdisciplina de la psicología o es un área en la que convergen distintas disciplinas. Conforme se avanzó hacia estos objetivos, fue imperante describir, de forma introductoria, las estrategias que se han implementado desde la psicología interconductual para resolver los problemas relacionados con la definición de desarrollo psicológico y, por lo tanto, con el objeto de estudio de la psicología. Partiendo de la definición de este objeto, fue posible distinguir entre el desarrollo humano como un área de conocimiento en la que convergen distintas disciplinas y el desarrollo psicológico como ámbito de la psicología, superando así las confusiones originadas del tomarlas como equivalentes. Finalmente, se definió el desarrollo psicológico con base en las competencias funcionales y se discutió la pertenencia de sustituir el concepto de desarrollo por el de devenir psicológico.


The present work had the purpose of pointing out and solving some of the conceptual problems related to the area of knowledge called psychological development. The first step in achieving this was to clarify whether psychological development is a sub-discipline of psychology or an area in which different disciplines converge. As objectives were reached, it was imperative to describe, in an introductory way, the strategies that have been implemented from interbehavioral psychology to solve the problems related to the definition of psychological development and, therefore, with the object of psychology study. Starting from the definition of this object, it was possible to distinguish between human development as an area of knowledge in which different disciplines converge and psychological development as an area of psychology, overcoming the confusions originated from taking them as equivalents. Finally, psychological development was defined based on functional competencies, and the relevance of replacing the concept of development with psychological becoming was discussed.

18.
Rev Colomb Psiquiatr ; 48(2): 96-104, 2019.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30981333

RESUMO

OBJECTIVE: Colombia is facing a rising epidemic of intravenous heroin use. Knowledge of the methadone-assisted treatment programs in the country is crucial in order to propose improvement strategies. METHODS: 13 programmes from priority regions were surveyed. The demographic and clinical characteristics of the patients attending the programs, a description of the services offered, their methadone treatment protocols, the various barriers to treatment and the causes of treatment abandonment were reviewed. RESULTS: 12/13 questionnaires were analysed with a total of 538 active patients. Most of the patients attending these programs were men (85.5%) between 18 and 34 years-old (70%). Forty percent (40%) were intravenous drug users and 25% admitted sharing needles. The comorbidities associated with heroin use were mental illness (48%), hepatitis C (8.7%) and HIV (2%). Psychiatric comorbidity was more likely in patients attending the private sector (69.8% vs 29.7%; p<0.03). The initial average dose of methadone administered was 25.3±8.9mg/day, with a maintenance dose ranging from 41 to 80mg/day. Lack of alignment with primary care was perceived to be the most serious barrier to access, ahead of problems with insurance and prejudice towards treatment with methadone (p<0.05). Health Administration and insurance problems (p<0.003), together with the lack of availability of methadone (p<0.018) and relapse (p<0.014) were the most important reasons for abandonment of treatment. CONCLUSIONS: The treatment protocols of these programmes offer different levels of development and implementation. Some of the barriers to access and reasons for abandonment of treatment with methadone can be mitigated with better health administration.


Assuntos
Acesso aos Serviços de Saúde , Dependência de Heroína/epidemiologia , Metadona/administração & dosagem , Abuso de Substâncias por Via Intravenosa/epidemiologia , Adolescente , Adulto , Criança , Colômbia , Feminino , Dependência de Heroína/reabilitação , Humanos , Masculino , Uso Comum de Agulhas e Seringas/estatística & dados numéricos , Tratamento de Substituição de Opiáceos/métodos , Abuso de Substâncias por Via Intravenosa/reabilitação , Inquéritos e Questionários , Adulto Jovem
19.
Biomedica ; 39(1): 65-74, 2019 03 31.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31021548

RESUMO

INTRODUCTION: Direct visual inspection for cervical cancer screening remains controversial, whereas colposcopy-biopsy is considered the gold standard for diagnosis of preneoplastic cervical lesions. OBJECTIVES: To determine the rates of cervical intraepithelial neoplasia grade 2 or more and of false positives for colposcopy and direct visual inspection. MATERIALS AND METHODS: Women aged 25-59 underwent direct visual inspection with acetic acid (VIA), Lugol's iodine (VIA-VILI), and colposcopy. Punch biopsies were obtained for all positive tests. Using histology as the gold standard, detection and false positive rates were compared for VIA, VIA-VILI, and colposcopy (two thresholds). Sensitivity and false positive ratios with the corresponding 95% confidence intervals were estimated. RESULTS: We included 5,011 women in the analysis and we obtained 602 biopsies. Positivity rates for colposcopy high-grade and low-grade diagnosis were 1.6% and 10.8%. Positivity rates for VIA and VIA-VILI were 7.4% and 9.9%. VIA showed a significantly lower detection rate than colposcopy with low-grade diagnosis as the threshold (SR=0.72; 95% CI 0.57-0.91), and significantly lower false positive rate (FPR=0.70; 95% CI 0.65-0.76). No differences between VIA-VILI and colposcopy low-grade threshold were observed. VIA and VIA-VILI showed significantly higher detection and false positive rates than colposcopy high-grade threshold. Sensitivity rates for visual inspection decreased with age and false positive rates increased. For all age groups, false positive rates for VIA and VIA-VILI were significantly higher than colposcopy. CONCLUSIONS: Detection rates for VIA-VILI similar to colposcopy low-grade threshold represent a chance to reduce cervical cancer mortality through see-and-treat approaches among women with limited access to health care. Lower detection rates suggest reviewing high-grade colposcopy findings as the threshold for biopsy in certain settings.


Assuntos
Neoplasia Intraepitelial Cervical/patologia , Detecção Precoce de Câncer/métodos , Neoplasias do Colo do Útero/patologia , Ácido Acético , Adulto , Biópsia , Colômbia , Colposcopia , Reações Falso-Positivas , Feminino , Humanos , Iodetos , Pessoa de Meia-Idade
20.
Front Microbiol ; 10: 612, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30984140

RESUMO

Listeria monocytogenes is a foodborne pathogen that can cause severe disease in susceptible humans. This microorganism has the ability to adapt to hostile environmental conditions such as the low temperatures used by the food industry for controlling microorganisms. Bacteria are able to adjust their transcriptional response to adapt to stressful conditions in order to maintain cell homeostasis. Understanding the transcriptional response of L. monocytogenes to stressing conditions could be relevant to develop new strategies to control the pathogen. A possible alternative for controlling microorganisms in the food industry could be to use copper as an antimicrobial agent. The present study characterized three L. monocytogenes strains (List2-2, Apa13-2, and Al152-2A) adapted to low temperature and challenged with different copper concentrations. Similar MIC-Cu values were observed among studied strains, but growth kinetic parameters revealed that strain List2-2 was the least affected by the presence of copper at 8°C. This strain was selected for a global transcriptional response study after a 1 h exposition to 0.5 mM of CuSO4 × 5H2O at 8 and 37°C. The results showed that L. monocytogenes apparently decreases its metabolism in response to copper, and this reduction is greater at 8°C than at 37°C. The most affected metabolic pathways were carbohydrates, lipids and nucleotides synthesis. Finally, 15 genes were selected to evaluate the conservation of the transcriptional response in the other two strains. Results indicated that only genes related to copper homeostasis showed a high degree of conservation between the strains studied, suggesting that a low number of genes is implicated in the response to copper stress in L. monocytogenes. These results contribute to the understanding of the molecular mechanisms used by bacteria to overcome a combination of stresses. This study concluded that the application of copper in low concentrations in cold environments may help to control foodborne pathogens as L. monocytogenes in the industry.

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