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1.
Endocrinol Metab (Seoul) ; 36(5): 1055-1068, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34674511

RESUMO

BACKGROUND: We studied whether musclin function in humans is related to glycemic control, body composition, and cardiorespiratory capacity. METHODS: A cross-sectional study was performed in sedentary adults with or without metabolic syndrome (MS). Serum musclin was measured by enzyme-linked immunosorbent assay. Insulin resistance (IR) was evaluated by the homeostatic model assessment (HOMA-IR). Body composition was determined by dual-energy X-ray absorptiometry and muscle composition by measuring carnosine in the thigh, a surrogate of fiber types, through proton magnetic resonance spectroscopy. Cardiorespiratory capacity was assessed through direct ergospirometry. RESULTS: The control (n=29) and MS (n=61) groups were comparable in age (51.5±6.5 years old vs. 50.7±6.1 years old), sex (72.4% vs. 70.5% women), total lean mass (58.5%±7.4% vs. 57.3%±6.8%), and peak oxygen consumption (VO2peak) (31.0±5.8 mL O2./kg.min vs. 29.2±6.3 mL O2/kg.min). Individuals with MS had higher body mass index (BMI) (30.6±4.0 kg/m2 vs. 27.4± 3.6 kg/m2), HOMA-IR (3.5 [95% confidence interval, CI, 2.9 to 4.6] vs. 1.7 [95% CI, 1.1 to 2.0]), and musclin (206.7 pg/mL [95% CI, 122.7 to 387.8] vs. 111.1 pg/mL [95% CI, 63.2 to 218.5]) values than controls (P˂0.05). Musclin showed a significant relationship with HOMA-IR (ß=0.23; 95% CI, 0.12 to 0.33; P˂0.01), but not with VO2peak, in multiple linear regression models adjusted for age, sex, fat mass, lean mass, and physical activity. Musclin was significantly associated with insulin, glycemia, visceral fat, and regional muscle mass, but not with BMI, VCO2peak, maximum heart rate, maximum time of work, or carnosine. CONCLUSION: In humans, musclin positively correlates with insulinemia, IR, and a body composition profile with high visceral adiposity and lean mass, but low body fat percentage. Musclin is not related to BMI or cardiorespiratory capacity.

2.
Eur J Appl Physiol ; 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34687360

RESUMO

PURPOSE: We carried out a randomized, clinical trial in adults of both sexes with metabolic syndrome (MS) to assess the efficacy of high-intensity, low-volume interval training (HIIT) compared to moderate-intensity continuous training (MICT) on insulin resistance (IR), muscle mass, muscle activation, and serum musclin. METHODS: Fasting glycemia, insulinemia, and glycated haemoglobin were determined by conventional methods, IR by Homeostatic model assessment (HOMA), lean mass by Dual-Energy X-ray Absorptiometry, muscle activation through carnosine by Proton Magnetic Resonance Spectroscopy, and musclin by Enzyme-Linked ImmunoSorbent Assay before and after a supervised, three-times/week, 12-week treadmill programme. HIIT (n = 29) consisted of six intervals with one-minute, high-intensity phases at 90% of peak oxygen consumption (VO2peak). MICT (n = 31) trained at 60% of VO2peak for 30 min. RESULTS: Patients had a mean age of 50.8 ± 6.0 years, body mass index of 30.6 ± 4.0 kg/m2, and VO2peak of 29.0 ± 6.3 mL.kg-1.min-1. Compared to MICT, HIIT was not superior at reducing Ln HOMA-IR (adjusted mean difference: 0.083 [95%CI - 0.092 to 0.257]), carnosine or musclin or at increasing thigh lean mass. HIIT increased carnosine by 0.66 mmol/kg.ww (95% CI 0.08-1.24) after intervention. Both interventions reduced IR, body fat percentage and increased total lean mass/height2 and VO2peak. Musclin showed a non-significant reduction with a small effect size after both interventions. CONCLUSION: Compared to MICT, HIIT is not superior at reducing IR, carnosine or musclin or at increasing skeletal muscle mass in adults with MS. Both training types improved IR, muscle mass and body composition. NCT03087721, March 22nd, 2017. TRIAL REGISTRATION NUMBER: NCT03087721. Registered March 22nd, 2017.

3.
Rev. colomb. gastroenterol ; 36(2): 241-251, abr.-jun. 2021. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1289304

RESUMO

Resumen Actualmente, la pandemia por la enfermedad por coronavirus de 2019 (COVID-19) ha sido uno de los grandes retos para el personal de la salud y se ha convertido en un mayor desafío cuando hay pacientes con enfermedad inflamatoria intestinal (EII); debido a la fisiopatología de la enfermedad y los tratamientos utilizados, se pueden generar interrogantes difíciles de contestar ante el corto tiempo de evidencia científica existente sobre la vacunación para coronavirus del síndrome respiratorio agudo grave de tipo 2 (SARS-CoV-2). Es claro que la vacunación es una de las intervenciones de salud pública más efectivas para lograr una inmunidad colectiva en esta pandemia; por tal motivo, se realiza una revisión enfocada en los aspectos claves frente a la vacunación para la infección por SARS-CoV-2 en los pacientes con EII según la evidencia disponible.


Abstract Currently, the coronavirus disease 2019 (COVID-19) pandemic has been one of the great challenges for health personnel and has become a greater challenge when we have patients with inflammatory bowel disease (IBD); because of the pathophysiology of the disease and the treatments used, it can generate questions that are difficult to answer given the short time of scientific evidence that we have on vaccination for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The vaccination is one of the most effective public health interventions to get immunity in this pandemic; for this reason, we made a review with the key aspects about vaccination for infection by SARS-CoV-2 in patients with IBD according to the available evidence.

4.
Pensam. psicol ; 18(2): 79-91, Jul.-Dec. 2020. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1154987

RESUMO

Resumen Objetivo. Explorar las diferencias entre los niveles de actividad física (AF) ‒ligero, moderado y vigoroso‒, en función a la Calidad de Vida Relacionada con la Salud (CvRS). Método. 269 participantes (M = 25.3, DE = 1.5) entre estudiantes, docentes y administrativos de una comunidad académica de Pereira, Colombia. La AF se evaluó con el International Physical Activity Questionnaire (IPAQ) y la CvRS con el Cuestionario de Salud SF-36. Resultados. Quienes reportaron tener un nivel de AF moderado mostraron significativamente mejor vitalidad, salud mental y salud en general (p < 0.05). Mientras que aquellos con nivel de AF vigoroso promediaron más alto en salud general. Los niveles de AF moderado y vigoroso, en comparación con el ligero, presentaron mayor puntaje en la salud general y mental (p < 0.05). Conclusión. Este estudio aportó evidencia a favor de la hipótesis del vínculo entre AF y CvRS; además, reveló que aquellos con niveles de AF moderado y vigoroso presentan mejor CvRS en dimensiones de función social, vitalidad y salud general y mental. Futuros estudios deberían enfocarse en diferenciar los niveles de AF que mejor favorecen la CvRS, principalmente, en población escolar .


Abstract Objective. To explore the Physical Activity (PA) levels ‒Low, Medium and High‒ differences according to Health-Related Quality of Life (HRQL). Method. 269 participants (M = 25.3, SD = 1.5) between, students, teachers and administrators of an academic community of Pereira, Colombia. The PA was assessed with the International Physical Activity Questionnaire (IPAQ) and the HRQL through the SF-36. Results. Those who reported having a moderate PA level showed significantly best vitality, mental health outcomes, and overall health (p < 0.05). While those with vigorous PA level averaged higher in overall health. The moderate and vigorous PA levels, compared to the low level, showed a higher score in general health and mental health (p < 0.05). Conclusion. This study provides evidence in favor of the link between PA and HRQL hypothesis; in addition, those subjects who self-report moderate and vigorous PA levels showed better HRQL in the social function, vitality, general health, and mental health dimensions. Future studies should emphasize the different PA levels that will favor the HRQL, principally, in school population.


Resumo Escopo. Explorar as diferenças entre os níveis de atividade física (AF) leve, moderado e vigoroso, em função à Qualidade de Vida Relacionada com a Saúde (CvRS). Metodologia. 269 participantes (M = 25.3, DE = 1.5) entre estudantes, docentes e administrativos de uma comunidade académica de Pereira, Colômbia. A AF foi avaliada com o International Physical Activity Questionnaire (IPAQ) e a CvRS com o Questionário de Saúde SF-36. Resultados. Quem reportou ter um nível de AF moderado mostrou significativamente melhor vitalidade, saúde mental e saúde em geral (p < 0.05). Enquanto que aqueles com nível de AF vigoroso tem de média maior saúde geral. Os níveis de AF moderado e vigoroso, em comparação com o leve apresentaram maior pontuação na saúde geral e mental (p < 0.05). Conclusão. Este estudo aportou provas a favor da hipóteses do vínculo entre AF e CvRS; além, revelou que aqueles com níveis de AF moderado e vigoroso apresentam melhor CvRS em dimensões de função social, vitalidade e saúde geral e mental. Futuros estudos deverão se enfocar em diferenciar os níveis de AF que melhor favorecem a CvRS, principalmente, em população escolar.

5.
PLoS One ; 15(10): e0238994, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33052938

RESUMO

Brain networks offers a new insight about connections between function and anatomical regions of human brain. We present results from brain networks built from functional magnetic resonance images during finger tapping paradigm. Pearson voxel-voxel correlation in time and frequency domains were performed for all subjects. Besides this standard framework we have implemented a new approach consisting in filtering the data with respect to the fMRI paradigm (finger tapping) in order to obtain a better understanding of the network involved in the execution of the task. The main topological graph measures have been compared in both cases: voxel-voxel correlation and voxel-paradigm filtering plus voxel-voxel correlation. With the standard voxel-voxel correlation a clearly free-scale network was obtained. On the other hand, when we prefiltered the paradigm we obtained two different kind of networks: 1) free-scale; 2) random-like. To our best knowledge, this behaviour is reported here for first time for brain networks. We suggest that paradigm signal prefiltering can provide more infomation about the brain networks.


Assuntos
Mapeamento Encefálico/métodos , Encéfalo/diagnóstico por imagem , Encéfalo/fisiologia , Imageamento por Ressonância Magnética/métodos , Adulto , Mapeamento Encefálico/estatística & dados numéricos , Conectoma/métodos , Conectoma/estatística & dados numéricos , Feminino , Humanos , Imageamento por Ressonância Magnética/estatística & dados numéricos , Masculino , Rede Nervosa/diagnóstico por imagem , Rede Nervosa/fisiologia , Oxigênio/sangue , Desempenho Psicomotor/fisiologia
6.
Eur J Pharmacol ; 851: 88-98, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30771349

RESUMO

Pomolic acid (PA) isolated from Licania pittieri has hypotensive effects in rats, inhibits human platelet aggregation and elicits endothelium-dependent relaxation in rat aortic rings. The present study was designed to investigate the effects of PA on cardiomyocytes. Trabeculae and enzymatically isolated cardiomyocytes from rats were used to evaluate the concentration-dependent effects of PA on cardiac muscle tension and excitation-contraction coupling (ECC) by recording Ca2+ transients reported with Fluo-3 and Fura-2, as well as L-type Ca2+ currents (LTCC). PA reduced the contractile force in rat cardiac trabeculae with an EC50 = 14.3 ±â€¯2.4 µM. PA also reduced the amplitude of Ca2+ transients in a concentration-dependent manner, with an EC50 = 10.5 ±â€¯1.3 µM, without reducing sarcoplasmic reticulum (SR) Ca2+ loading. PA decreased the half width of the Ca2+ transient by 31.7 ±â€¯3.3% and increased the decay time and decay time constant (τ) by 7.6 ±â€¯2.7% and 75.6 ±â€¯3.7%, respectively, which was associated with increased phospholamban (PLN) phosphorylation. PA also reversibly reduced the macroscopic LTCC in the cardiomyocyte membrane, but did not demonstrate any effects on skeletal muscle ECC. In conclusion, PA reduces LTCC, Ca2+ transients and cardiomyocyte force, which along with its vasorelaxant effects explain its hypotensive properties. Increased PLN phosphorylation protected the SR from Ca2+ depletion. Considering the effects of PA on platelet aggregation and the cardiovascular system, we propose it as a new potential, multitarget cardiovascular agent with a demonstrated safety profile.


Assuntos
Acoplamento Excitação-Contração/efeitos dos fármacos , Contração Miocárdica/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Ácido Oleanólico/análogos & derivados , Animais , Canais de Cálcio Tipo L/metabolismo , Masculino , Miócitos Cardíacos/citologia , NG-Nitroarginina Metil Éster/farmacologia , Ácido Oleanólico/farmacologia , Fosforilação/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Retículo Sarcoplasmático/efeitos dos fármacos , Retículo Sarcoplasmático/metabolismo
7.
Front Psychol ; 10: 2774, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31920823

RESUMO

Physical exercise (PE) is associated with cognitive changes and brain function. However, it is required to clarify the effect of PE in different intensities, population groups conditions and the EF duration over different cognitive domains. Besides, no studies are known to have evaluated the contextual emotional recognition. Therefore, we studied the effect of acute PE of moderate intensities up to higher ones to the executive functions and the contextual emotional recognition. The participants were evaluated and classified in two experiments according to the IPAQ short form self-report and control measures. In both experiments, the groups were randomized, controlled, and exposed to one session of indoor cycling through intervals of high measure intensity (75-85% HRmax). Experiment 1 comprised young adults who were physically active (PA) and healthy, apparently (n = 54, M age = 20.7, SD = 2.5). Experiment 2 involved young adults who were physically inactive (IP) and healthy, apparently (n = 36, M age = 21.6, SD = 1.8). The duration was the only factor that varied: 45 min for PA and 30 min for PI. The executive functions were evaluated by the Stroop, TMT A/B, and verbal fluency, and the emotional recognition through a task that includes body and facial emotions in context, simultaneously. The analysis of factorial mixed ANOVA showed effects on the right choices of the indoor cycling groups in the PA, and the time response in PI. Also, other effects were observed in the controlled groups. TMT-A/B measures showed changes in the pre-test-post-test measures for both experiments. Verbal fluency performance favored the control group in both experiments. Meanwhile, the emotional recognition showed an effect of the PE in error-reduction and enhanced the scores in the right choices of body emotions. These results suggest that the EF with intensities favored cognitive processes such as inhibitory control and emotional recognition in context. We took into account the importance of high-complexity tasks design that avoid a ceiling effect. This study is the first on reporting a positive effect of PE over the emotional contextual recognition. Important clinical and educational implications are presented implications which highlight the modulatory role of EF with moderate to high intensities.

8.
J Natl Cancer Inst ; 109(6)2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28040796

RESUMO

Background: Metaplastic breast cancer is one of the most therapeutically challenging forms of breast cancer because of its highly heterogeneous and chemoresistant nature. We have previously demonstrated that ribosomal protein L39 (RPL39) and its gain-of-function mutation A14V have oncogenic activity in triple-negative breast cancer and this activity may be mediated through inducible nitric oxide synthase (iNOS). The function of RPL39 and A14V in other breast cancer subtypes is currently unknown. The objective of this study was to determine the role and mechanism of action of RPL39 in metaplastic breast cancer. Methods: Both competitive allele-specific and droplet digital polymerase chain reaction were used to determine the RPL39 A14V mutation rate in metaplastic breast cancer patient samples. The impact of RPL39 and iNOS expression on patient overall survival was estimated using the Kaplan-Meier method. Co-immunoprecipitation and immunoblot analyses were used for mechanistic evaluation of RPL39. Results: The RPL39 A14V mutation rate was 97.5% (39/40 tumor samples). High RPL39 (hazard ratio = 0.71, 95% confidence interval = 0.55 to 0.91, P = 006) and iNOS expression (P = 003) were associated with reduced patient overall survival. iNOS inhibition with the pan-NOS inhibitor NG-methyl-L-arginine acetate decreased in vitro proliferation and migration, in vivo tumor growth in both BCM-4664 and BCM-3807 patient-derived xenograft models (P = 04 and P = 02, respectively), and in vitro and in vivo chemoresistance. Mechanistically, RPL39 mediated its cancer-promoting actions through iNOS signaling, which was driven by the RNA editing enzyme adenosine deaminase acting on RNA 1. Conclusion: NOS inhibitors and RNA editing modulators may offer novel treatment options for metaplastic breast cancer.


Assuntos
Inibidores Enzimáticos/uso terapêutico , Óxido Nítrico Sintase Tipo II/metabolismo , Proteínas Ribossômicas/genética , Proteínas Ribossômicas/metabolismo , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/patologia , ômega-N-Metilarginina/uso terapêutico , Adenosina Desaminase/genética , Adenosina Desaminase/metabolismo , Animais , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Movimento Celular/genética , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/genética , Regulação para Baixo/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Feminino , Humanos , Estimativa de Kaplan-Meier , Metaplasia , Camundongos , Taxa de Mutação , Transplante de Neoplasias , Nitratos/metabolismo , Óxido Nítrico Sintase Tipo II/antagonistas & inibidores , Óxido Nítrico Sintase Tipo II/genética , Nitritos/metabolismo , RNA Interferente Pequeno/farmacologia , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais/genética , Taxa de Sobrevida , Neoplasias de Mama Triplo Negativas/metabolismo , Ubiquitina C/metabolismo , ômega-N-Metilarginina/farmacologia
9.
Adv Pharmacol Sci ; 2015: 418673, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26366171

RESUMO

Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine.

10.
Molecules ; 20(4): 6181-93, 2015 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-25859777

RESUMO

Jatropha gossypifolia L. (Euphorbiaceae) is a plant widely used in the treatment of type 2 diabetes mellitus (T2DM), but there are few scientific reports validating its activity in this area. In this work and through a bioguided assay, a crude extract stimulated glucose uptake in C2C12 myotubes up to 30%, thereby reducing insulin resistance induced by fatty acids compared to the basal control. A chromatographic fraction applied intraperitoneally (IP) in mice reduced glucose by 42% in a mouse model of T2DM, after administration of 10 doses during 20 days. A flavanone was purified from this active fraction and its structure was assigned by 1H- and 13C-NMR (1D and 2D) and MS. This compound retains the previously reported activity, stimulating in vitro the glucose uptake in a concentration-dependent manner. This study indicates that Jatropha gossypifolia L. extracts enhance glucose uptake in cultured myotubes and adipocytes and also improving glucose tolerance in an in vivo model.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Flavonoides/administração & dosagem , Hipoglicemiantes/administração & dosagem , Extratos Vegetais/administração & dosagem , Animais , Glicemia , Diabetes Mellitus Tipo 2/patologia , Flavonoides/química , Teste de Tolerância a Glucose , Humanos , Hipoglicemiantes/química , Jatropha/química , Camundongos , Extratos Vegetais/química
11.
Stem Cells ; 32(9): 2309-23, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24809620

RESUMO

Triple negative breast cancer (TNBC) is known to contain a high percentage of CD44(+) /CD24(-/low) cancer stem cells (CSCs), corresponding with a poor prognosis despite systemic chemotherapy. Chloroquine (CQ), an antimalarial drug, is a lysotropic reagent which inhibits autophagy. CQ was identified as a potential CSC inhibitor through in silico gene expression signature analysis of the CD44(+) /CD24(-/low) CSC population. Autophagy plays a critical role in adaptation to stress conditions in cancer cells, and is related with drug resistance and CSC maintenance. Thus, the objectives of this study were to examine the potential enhanced efficacy arising from addition of CQ to standard chemotherapy (paclitaxel) in TNBC and to identify the mechanism by which CQ eliminates CSCs in TNBCs. Herein, we report that CQ sensitizes TNBC cells to paclitaxel through inhibition of autophagy and reduces the CD44(+) /CD24(-/low) CSC population in both preclinical and clinical settings. Also, we are the first to report a mechanism by which CQ regulates the CSCs in TNBC through inhibition of the Janus-activated kinase 2 (Jak2)-signal transducer and activator of transcription 3 signaling pathway by reducing the expression of Jak2 and DNA methyltransferase 1.


Assuntos
Cloroquina/farmacologia , DNA (Citosina-5-)-Metiltransferases/metabolismo , Janus Quinase 2/metabolismo , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Neoplasias de Mama Triplo Negativas/patologia , Animais , Autofagia/efeitos dos fármacos , Linhagem Celular Tumoral , DNA (Citosina-5-)-Metiltransferase 1 , Feminino , Humanos , Camundongos , Camundongos Nus , Células-Tronco Neoplásicas/patologia , Transdução de Sinais/efeitos dos fármacos , Neoplasias de Mama Triplo Negativas/metabolismo
12.
Free Radic Biol Med ; 47(7): 924-31, 2009 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-19539747

RESUMO

The pathophysiology of nonalcoholic steatohepatitis (NASH) is still not fully understood, and available treatments are not entirely satisfactory. Steatosis progression to NASH is associated with deleterious action of reactive oxygen species, mitochondrial dysfunction, and inflammatory cytokines. We investigated the use of curcumin (compared to vitamin E) in the treatment of NASH. Experimental NASH was induced in rabbits by the intake of a high-fat diet. Oxidative stress status, histology, lipid metabolism, and TNF-alpha protein levels were assessed in liver. The high-fat diet induced pathologically assessed NASH, and compared to healthy controls, raised the levels of aminotransferases, reduced mitochondrial antioxidants, increased mitochondrial reactive oxygen species, and led to poor mitochondrial function as well as to higher TNF-alpha protein levels. Curcumin administration together with the high-fat diet led to rabbits with a lower NASH grade and lower levels of aminotransferases, higher values for mitochondrial antioxidants, lower mitochondrial reactive oxygen species, an improved mitochondrial function, and lower levels of TNF-alpha protein levels. Vitamin E treatment was unable to reduce NASH. In conclusion, curcumin might be useful in the management of NASH through a mechanism involving the antioxidant, anti-inflammatory, and mitochondrial-protective potential of curcumin.


Assuntos
Curcumina/farmacologia , Fígado Gorduroso/tratamento farmacológico , Mitocôndrias/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismo , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Antioxidantes/farmacologia , Gorduras na Dieta/administração & dosagem , Gorduras na Dieta/efeitos adversos , Transporte de Elétrons/efeitos dos fármacos , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Fígado Gorduroso/metabolismo , Fígado Gorduroso/patologia , Fígado/metabolismo , Fígado/patologia , Masculino , Mitocôndrias/metabolismo , Fosfolipídeos/análise , Coelhos , Espécies Reativas de Oxigênio/metabolismo
13.
Biofactors ; 32(1-4): 263-73, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19096124

RESUMO

The effects of the administration of water soluble coenzyme Q10 (25 mg/kg per day) over 30 days, after 50 days feeding on a high-fat diet (3% lard + 1.3% cholesterol), were investigated in the plasma and liver mitochondria of rabbits. Results showed that this atherogenic diet enhanced lipid levels both in plasma and liver mitochondria, reduced plasma and mitochondrial concentrations of retinol and coenzyme Q10, led to higher DNA damage in peripheral blood lymphocytes and reactive oxygen species concentration in liver mitochondria. The treatment of animals with coenzyme Q10 reduced (to the healthy group levels) lipid concentration in liver mitochondria with no effect on plasma lipids, increased mitochondrial levels of alpha-tocopherol, restored mitochondrial coenzyme Q10 and improved alpha-tocopherol levels in plasma. Moreover, coenzyme Q10 supplementation reduced mitochondrial reactive oxygen species levels and decreased DNA damage in peripheral blood lymphocytes. The findings suggest that antioxidant therapy with coenzyme Q10 may be used in the treatment of liver pathologies associated to the intake of high-fat, atherogenic, diets.


Assuntos
Aterosclerose/metabolismo , Dano ao DNA , Linfócitos/metabolismo , Mitocôndrias Hepáticas/metabolismo , Estresse Oxidativo/fisiologia , Ubiquinona/análogos & derivados , Animais , Dieta Aterogênica , Linfócitos/efeitos dos fármacos , Masculino , Modelos Animais , Coelhos , Espécies Reativas de Oxigênio/metabolismo , Ubiquinona/farmacologia , Vitamina A/sangue
14.
Nutrition ; 24(11-12): 1167-73, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18586460

RESUMO

OBJECTIVE: Oxidative stress results from an imbalance between the formation and neutralization of pro-oxidants. Disturbance of the pro-oxidant/antioxidant balance is also considered to be a causative factor underlying oxidative damage to cellular molecules, such as DNA, causing strand breaks. There is considerable controversy about the antioxidant status in iron-deficiency anemia (IDA), but scant information is available regarding DNA integrity. In the present study, we investigated the relation between DNA stability and hepatic antioxidant capacity in rats with induced IDA. METHODS: Peripheral DNA damage was assessed using an alkaline comet assay. Further, the hepatic antioxidant enzyme glutathione peroxidase and the production of thiobarbituric acid-reactive substances were measured in control rats and in those with induced IDA. RESULTS: Comparison of the control and anemic rats showed no differences in thiobarbituric acid-reactive substances production in the cytosolic fraction of hepatic cells. Nor were there any differences in liver glutathione peroxidase enzyme activity or DNA stability, as demonstrated by the percentage of DNA in the head (90.77 in control rats versus 88.23 in anemic rats), tail (9.23 in control rats versus 11.76 in anemic rats), and olive tail moment (0.155 in control rats versus 0.141 in anemic rats). CONCLUSION: IDA does not affect DNA stability or lipid peroxidation in rats, suggesting that there is enough compensatory capacity to keep antioxidant defenses high.


Assuntos
Anemia Ferropriva/sangue , Antioxidantes/metabolismo , Dano ao DNA , Glutationa Peroxidase/metabolismo , Peroxidação de Lipídeos , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Anemia Ferropriva/fisiopatologia , Animais , Ensaio Cometa/métodos , Peroxidação de Lipídeos/fisiologia , Fígado/enzimologia , Masculino , Oxirredução , Estresse Oxidativo , Distribuição Aleatória , Ratos , Ratos Wistar
15.
Rev. colomb. anestesiol ; 24(1): 123-4, ene.-mar. 1996.
Artigo em Espanhol | LILACS | ID: lil-218112
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