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1.
Front Cardiovasc Med ; 9: 864188, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35509278

RESUMO

Thoracic radiotherapy patients have higher risks of developing radiation-induced heart disease (RIHD). Ionizing radiation generates excessive reactive oxygens species (ROS) causing oxidative stress, while Momordica. charantia and its extract have antioxidant activity. Plant-derived extracellular vesicles (EVs) is emerging as novel therapeutic agent. Therefore, we explored the protective effects of Momordica. charantia-derived EVs-like nanovesicles (MCELNs) against RIHD. Using density gradient centrifugation, we successfully isolated MCELNs with similar shape, size, and markers as EVs. Confocal imaging revealed that rat cardiomyocytes H9C2 cells internalized PKH67 labeled MCELNs time-dependently. In vitro assay identified that MCELNs promoted cell proliferation, suppressed cell apoptosis, and alleviated the DNA damage in irradiated (16 Gy, X-ray) H9C2 cells. Moreover, elevated mitochondria ROS in irradiated H9C2 cells were scavenged by MCELNs, protecting mitochondria function with re-balanced mitochondria membrane potential. Furthermore, the phosphorylation of ROS-related proteins was recovered with increased ratios of p-AKT/AKT and p-ERK/ERK in MCELNs treated irradiated H9C2 cells. Last, intraperitoneal administration of MCELNs mitigated myocardial injury and fibrosis in a thoracic radiation mice model. Our data demonstrated the potential protective effects of MCELNs against RIHD. The MCELNs shed light on preventive regime development for radiation-related toxicity.

2.
Chin Med ; 17(1): 51, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35477576

RESUMO

BACKGROUND: Hemorrhagic transformation (HT) is a common complication of delayed tissue plasminogen activator (t-PA) treatment for ischemic stroke. Peroxynitrite plays an important role in the breakdown of blood-brain barrier (BBB) and the development of HT. We tested the hypothesis that Angong Niuhuang Wan (AGNHW), a traditional Chinese medicinal formula, could be used in conjunction with t-PA to protect the BBB, minimize HT, and improve neurological function by suppressing peroxynitrite-mediated matrix metalloproteinase-9 (MMP-9) activation. METHODS: We first performed quality control study and chemical identification of AGNHW by using UPLC. In animal experiments, male Sprague-Dawley rats were subjected to 5 h of middle cerebral artery occlusion (MCAO) followed by 19 h of reperfusion plus t-PA infusion (10 mg/kg) at 5 h of cerebral ischemia. AGNHW (257 mg/kg) was given orally at 2 h after MCAO. Hemorrhagic transformation was measured using hemorrhagic scores and hemoglobin levels in ischemic brains. Evans blue leakage was utilized to assess the severity of the blood-brain barrier (BBB) damage. The modified neurologic severity score (mNSS) test was used to assess neurological functions. Peroxynitrite and superoxide was detected by using fluorescent probes. MMP-9 activity and expression were examined by gelatin zymography and immunostaining. The antioxidant effects were also studied by using brain microvascular endothelial b.End3 cells exposed to 5 h of oxygen and glucose deprivation (OGD) plus 5 h of reoxygenation with t-PA treatment (20 µg/ml). RESULTS: AGNHW significantly reduced the BBB damage, brain edema, reduced hemorrhagic transformation, enhanced neurological function, and reduced mortality rate in the ischemic stroke rats with t-PA treatment. AGNHW reduced peroxynitrite and superoxide in vivo and in vitro and six active chemical compounds were identified from AGNHW with peroxynitrite scavenging activity. Furthermore, AGNHW inhibited MMP-9 activity, and preserved tight junction protein claudin-5 and collagen IV in the ischemic brains. CONCLUSION: AGNHW could be a potential adjuvant therapy with t-PA to protect the BBB integrity, reduce HT, and improve therapeutic outcome in ischemic stroke treatment via inhibiting peroxynitrite-mediated MMP-9 activation.

3.
Front Microbiol ; 13: 843417, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35464991

RESUMO

With its low-cost, label-free and non-destructive features, Raman spectroscopy is becoming an attractive technique with high potential to discriminate the causative agent of bacterial infections and bacterial infections per se. However, it is challenging to achieve consistency and accuracy of Raman spectra from numerous bacterial species and phenotypes, which significantly hinders the practical application of the technique. In this study, we analyzed surfaced enhanced Raman spectra (SERS) through machine learning algorithms in order to discriminate bacterial pathogens quickly and accurately. Two unsupervised machine learning methods, K-means Clustering (K-Means) and Agglomerative Nesting (AGNES) were performed for clustering analysis. In addition, eight supervised machine learning methods were compared in terms of bacterial predictions via Raman spectra, which showed that convolutional neural network (CNN) achieved the best prediction accuracy (99.86%) with the highest area (0.9996) under receiver operating characteristic curve (ROC). In sum, machine learning methods can be potentially applied to classify and predict bacterial pathogens via Raman spectra at general level.

4.
J Am Chem Soc ; 144(17): 7758-7767, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35404593

RESUMO

Optical cavities provide a versatile platform for manipulating the excited-state dynamics of molecules via strong light-matter coupling. We employ optical absorption and two-multidimensional electronic spectroscopy simulations to investigate the effect of optical cavity coupling in the nonadiabatic dynamics of photoexcited pyrazine. We observe the emergence of a novel polaritonic conical intersection (PCI) between the electronic dark state and photonic surfaces as the cavity frequency is tuned. The PCI could significantly change the nonadiabatic dynamics of pyrazine by doubling the decay rate constant of the S2 state population. Moreover, the absorption spectrum and excited-state dynamics could be systematically manipulated by tuning the strong light-matter interaction, e.g., the cavity frequency and cavity coupling strength. We propose that a tunable optical cavity-molecule system may provide promising approaches for manipulating the photophysical properties of molecules.

5.
Biochem Biophys Res Commun ; 609: 84-92, 2022 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-35421633

RESUMO

Autophagy is a double-edged sword that affects tumor progression by promoting cell survival or death depending on different living contexts. The concrete mechanism by which autophagy modulates the efficacy of radiotherapy for prostate cancer (PC) remains unclear. We exposed RM-1 PC cells to X-ray and explored the role of autophagy in radiation injury. Our results showed increased apoptosis and autophagy levels in RM-1 cells after radiation. Pharmacological inhibition of autophagy by chloroquine significantly mitigated radiation-induced apoptosis, while the enhancement of autophagy by rapamycin aggravated apoptosis. Sirt1, a member of sirtuin family, deacetylates various transcription factors to trigger cell survival in response to radiation injury. We found that radiation led to Sirt1 downregulation, which was reversed by the inhibition of autophagy. On the contrary, enhanced autophagy further diminished protein level of Sirt1. Notably, overexpression of Sirt1 by plasmid significantly alleviated radiation-induced apoptosis, but silenced Sirt1 by siRNA further induced apoptosis, indicating the radioprotective effect of Sirt1 on RM-1 cells. In summary, our findings suggested that autophagy-mediated Sirt1 downregulation might be a promising therapeutic target for PC.


Assuntos
Neoplasias da Próstata , Lesões por Radiação , Sirtuína 1/metabolismo , Animais , Apoptose , Autofagia , Regulação para Baixo , Humanos , Masculino , Camundongos , Neoplasias da Próstata/genética , Neoplasias da Próstata/radioterapia , Tolerância a Radiação , Sirtuína 1/genética
6.
Am J Trop Med Hyg ; 2022 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-35405641

RESUMO

Brucellosis is a zoonotic disease with more than half a million people diagnosed worldwide. In China, 99% of cases were historically reported in the northern part of the country, and few were diagnosed in Guangdong province. Recently, Guangzhou has become as an emerging focus for brucellosis, with personal awareness of brucellosis and inexperience of clinicians hindering timely clinical diagnosis. To improve clinical management of this disease, we retrospectively analyzed 60 brucellosis cases from 2010 to 2020 in Guangdong Provincial People's Hospital. There were no manifestation differences between southern and northern patients. However, 68.3% of patients lacked awareness of risk factors for Brucella infection. Therefore, to prevent its spread and avoid delays in diagnosis, implemented infected-animal control programs and enhanced education on brucellosis are necessary.

7.
Biosens Bioelectron ; 206: 114150, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35278850

RESUMO

Despite their potential for signal amplification in immunochromatographic assays (ICAs) with Au nanoparticles (AuNPs) as probes, metal growth methods are of limited practical applicability given their complex non-specificity and lack of robust growth schemes. Here, we propose a novel method of polyallylamine hydrochloride (PAH)-mediated metal growth for the detection of Escherichia coli O157:H7 by AuNP-ICA. The developed method relies on the highly controlled growth of Cu shells on the AuNP core and allows one to achieve highly enhanced colorimetric signals by controlling PAH as the growth framework. The introduction of PAH eliminates the non-specific adsorption of Cu ions on the nitrocellulose membrane and thus provides maximized and effective signal-to-noise ratios to achieve a detection limit of 9.8 CFU/mL for E. coli O157:H7. Moreover, the newly developed detection method exhibits good reproducibility (coefficient of variation <13%), remarkable stability, and practical applicability. The PAH-mediated signal enhancement system paves the way to the realization of stable metal growth methods based on Au, Ag, and other metals and is well suited for the rapid, stable, and sensitive detection of food-borne pathogens using the AuNP-ICA platform.


Assuntos
Técnicas Biossensoriais , Escherichia coli O157 , Nanopartículas Metálicas , Microbiologia de Alimentos , Ouro/química , Imunoensaio , Nanopartículas Metálicas/química , Reprodutibilidade dos Testes
8.
Emerg Microbes Infect ; 11(1): 978-987, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35293849

RESUMO

The highly infectious Delta variant strain of SARS-CoV-2 remains globally dominant and undermines COVID-19 vaccines. Rapid detection of the Delta variant is crucial for the identification and quarantine of infected individuals. In this study, our aim was to design and validate a genotyping RT-LAMP method to detect Delta variants specifically. R203M in the N gene of SARS-CoV-2 was chosen as the Delta variant-specific mutation for genotyping. To target the R203M-harboring region and the conserved sequence of the N gene, two sets of primers were designed, and a Cq (quantification cycle) ratio-based RT-LAMP for SARS-CoV-2 and R203M detection was developed by analyzing the significant discrepancy in amplification efficiency of the two sets of primers. We validated the RT-LAMP method on 498 clinical specimens in parallel with RT-qPCR, and 84 Delta variants from 198 positive samples were determined by sequencing. Compared with traditional RT-qPCR analyses, RT-LAMP appears to be 100% accurate in detecting SARS-CoV-2 clinical samples. RT-LAMP has a good ability to distinguish between Delta and non-Delta variants under a Cq ratio threshold of 1.80. Furthermore, the AUC (area under the curve) of this method was 1.00; the sensitivity, specificity and accuracy were all 100%. In summary, we have proposed a rapid, accurate and cost-effective RT-LAMP method to detect SARS-CoV-2 and Delta variants, which may facilitate the surveillance of COVID-19.


Assuntos
COVID-19 , SARS-CoV-2 , COVID-19/diagnóstico , Vacinas contra COVID-19 , Humanos , Técnicas de Diagnóstico Molecular , Mutação , Técnicas de Amplificação de Ácido Nucleico , SARS-CoV-2/genética , Sensibilidade e Especificidade
9.
Chem Sci ; 13(4): 1037-1048, 2022 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-35211270

RESUMO

By placing Mg-porphyrin molecules in a chiral optical cavity, time reversal symmetry is broken, and polariton ring currents can be generated with linearly polarized light, resulting in a circular dichroism signal. Since the electronic state degeneracy in the molecule is lifted by the formation of chiral polaritons, this signal is one order of magnitude stronger than the bare molecule signal induced by circularly polarized light. Enantiomer-selective photochemical processes in chiral optical cavities is an intriguing future possibility.

10.
ACS Appl Mater Interfaces ; 14(6): 8282-8296, 2022 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-35112830

RESUMO

Hierarchical, ultrathin, and porous NiMoO4@CoMoO4 on Co3O4 hollow bones were successfully designed and synthesized by a hydrothermal route from the Co-precursor, followed by a KOH (potassium hydroxide) activation process. The hydrothermally synthesized Co3O4 nanowires act as the scaffold for anchoring the NiMoO4@CoMoO4 units but also show more compatibility with NiMoO4, leading to high conductivity in the heterojunction. The intriguing morphological features endow the hierarchical Co3O4@NiMoO4@CoMoO4 better electrochemical performance where the capacity of the Co3O4@NiMoO4@CoMoO4 heterojunction being 272 mA·h·g-1 at 1 A·g-1 can be achieved with a superior retention of 84.5% over 1000 cycles. The enhanced utilization of single/few NiMoO4@CoMoO4 shell layers on the Co3O4 core make it easy to accept extra electrons, enhancing the adsorption of OH- at the shell surface, which contribute to the high capacity. In our work, an asymmetric supercapacitor utilizing the optimized Co3O4@NiMoO4@CoMoO4 activated carbon (AC) as electrode materials was assembled, namely, Co3O4@NiMoO4@CoMoO4//AC device, yielding a maximum high energy density of 53.9 W·h·kg-1 at 1000 W·kg-1. It can retain 25.92 W·h·kg-1 even at 8100 W·kg-1, revealing its potential and viability for applications. The good power densities are ascribed to the porous feature from the robust architecture with recreated abundant mesopores on the composite, which assure improved conductivity and enhanced diffusion of OH- and also the electron transport. The work demonstrated here holds great promise for synthesizing other heterojunction materials M3O4@MMoO4@MMoO4 (M = Fe, Ni, Sn, etc).

11.
Opt Express ; 30(4): 5121-5130, 2022 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-35209481

RESUMO

Spin angular momentum (SAM) is widely used in spin-dependent unidirectional optical interfaces, optical manipulation, integrated optical signal processing, laser structuring and other fields, but its physical mechanism has not been fully understood so far. In this work, we investigate the three-dimensional (3D) SAM in tightly focused x-polarized first-order vortex beams from the perspectives of light field itself, phase distribution, and focusing propagation. It is shown that the distribution of three orthogonal components of SAM at the focal plane has pseudo two-fold rotational symmetry, because the cycloidal rotation of the electric field of the tightly focused vortex beam is opposite. The 3D SAM distribution in the focal region is visualized by mapping the 3D distribution of state of polarization (SoP). In addition, a principle experimental method for identifying the transverse SAM by using the direction of particle's rotation axis in optical tweezers is proposed.

12.
Microbiol Spectr ; 10(1): e0240921, 2022 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-35107359

RESUMO

In clinical settings, rapid and accurate diagnosis of antibiotic resistance is essential for the efficient treatment of bacterial infections. Conventional methods for antibiotic resistance testing are time consuming, while molecular methods such as PCR-based testing might not accurately reflect phenotypic resistance. Thus, fast and accurate methods for the analysis of bacterial antibiotic resistance are in high demand for clinical applications. In this pilot study, we isolated 7 carbapenem-sensitive Klebsiella pneumoniae (CSKP) strains and 8 carbapenem-resistant Klebsiella pneumoniae (CRKP) strains from clinical samples. Surface-enhanced Raman spectroscopy (SERS) as a label-free and noninvasive method was employed for discriminating CSKP strains from CRKP strains through computational analysis. Eight supervised machine learning algorithms were applied for sample analysis. According to the results, all supervised machine learning methods could successfully predict carbapenem sensitivity and resistance in K. pneumoniae, with a convolutional neural network (CNN) algorithm on top of all other methods. Taken together, this pilot study confirmed the application potentials of surface-enhanced Raman spectroscopy in fast and accurate discrimination of Klebsiella pneumoniae strains with different antibiotic resistance profiles. IMPORTANCE With the low-cost, label-free, and nondestructive features, Raman spectroscopy is becoming an attractive technique with great potential to discriminate bacterial infections. In this pilot study, we analyzed surfaced-enhanced Raman spectroscopy (SERS) spectra via supervised machine learning algorithms, through which we confirmed the application potentials of the SERS technique in rapid and accurate discrimination of Klebsiella pneumoniae strains with different antibiotic resistance profiles.


Assuntos
Antibacterianos/farmacologia , Carbapenêmicos/farmacologia , Farmacorresistência Bacteriana , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Análise Espectral Raman/métodos , Análise Discriminante , Humanos , Klebsiella pneumoniae/química , Klebsiella pneumoniae/genética , Aprendizado de Máquina , Testes de Sensibilidade Microbiana , Redes Neurais de Computação , Projetos Piloto
13.
J Control Release ; 341: 487-497, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34856228

RESUMO

Implantable insulin infusion systems using the intra-peritoneal route have dramatically changed the management of diabetes paving the way toward the realization of the potential "holy grail" of a fully implantable artificial pancreas. However, the wear duration of delivery catheters is compromised by the foreign body-mediated immune response. Both occlusion material present at the distal catheter tip end and fibrotic encapsulation surrounding the catheters influence the controlled and precise delivery of insulin, which eventually leads to the need for surgical intervention. The novel part of the current work is the investigation of the roles of implant physical properties (catheter size and tip configuration), as well as local inflammation control (through utilization of an anti-inflammatory agent) on the host fibrotic response using a previously developed animal model. The cellular and molecular response, the medication delivery efficacy as well as the ability to flush the catheters were examined and further compared among the different mitigation strategies. Reduction in catheter size as well as tuning the tip configuration from a cone shape to a round shape showed delayed host recognition and delayed propagation of the fibrotic response. However, the round shaped tips had an increased occurrence of lumen occlusion as a result of flow change. It became apparent that changing the physical properties of the catheters was not a long-term solution to catheter obstructions caused by the foreign body reaction. In comparison, control of the local inflammatory response through the use of an anti-inflammatory agent demonstrated a promising strategy for maintenance of catheter functionality without any type of obstructions. These finding will have a large impact toward the development of long-term use catheters for continuous intraperitoneal insulin infusion.


Assuntos
Diabetes Mellitus Tipo 1 , Insulina , Animais , Cateteres de Demora , Diabetes Mellitus Tipo 1/tratamento farmacológico , Reação a Corpo Estranho/tratamento farmacológico , Reação a Corpo Estranho/prevenção & controle , Sistemas de Infusão de Insulina
14.
J Chem Theory Comput ; 18(1): 406-414, 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-34920666

RESUMO

Quantum light spectroscopy, providing novel molecular information nonaccessible by classical light, necessitates new computational tools when applied to complex molecular systems. We introduce two computational protocols for the molecular nuclear wave packet dynamics interacting with an entangled photon pair to produce an entangled two-photon absorption signal. The first involves summing over transition pathways in a temporal grid defined by two light-matter interaction times accompanied by the field correlation functions of quantum light. The signal is obtained by averaging over the two time distribution characteristics of the entangled photon state. The other protocol involves a Schmidt decomposition of the entangled light and requires summing over the Schmidt modes. We demonstrate how photon entanglement can be used to control and manipulate the two-photon excited nuclear wave packets in a displaced harmonic oscillator model.

15.
Talanta ; 236: 122886, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635266

RESUMO

Rheumatoid arthritis (RA), an autoimmune and chronic inflammatory disorder, is an incurable disease. We developed a peptide-based electrochemical sensor using electrochemical impedance spectroscopy that can be used to detect autoantibodies for RA diagnostics. We first validated that the developed peptide showed high sensitivity and could compliment the current gold standard method of an anti-cyclic citrullinated peptide antibody (anti-CCP) ELISA. The developed peptide can be modified on the nanogold surface of the working electrode of sensing chips through the method of a self-assembling monolayer. The sensing process was first optimized using a positive control cohort and a healthy control cohort. Subsequently, 10 clinically confirmed samples from RA patients and five healthy control samples were used to find the threshold value of the impedance between RA and healthy subjects. Furthermore, 10 clinically confirmed samples but with low values of anti-CCP autoantibodies were used to evaluate the sensitivity of the present method compared to the conventional method. The proposed method showed better sensitivity than the current conventional anti-CCP ELISA method.


Assuntos
Artrite Reumatoide , Artrite Reumatoide/diagnóstico , Espectroscopia Dielétrica , Impedância Elétrica , Ensaio de Imunoadsorção Enzimática , Humanos , Peptídeos
16.
Light Sci Appl ; 10(1): 243, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34876551

RESUMO

Highly sensitive force measurements of a single microscopic particle with femto-Newton sensitivity have remained elusive owing to the existence of fundamental thermal noise. Now, researchers have proposed an optically controlled hydrodynamic manipulation method, which can measure the weak force of a single microscopic particle with femto-Newton sensitivity.

17.
Wideochir Inne Tech Maloinwazyjne ; 16(4): 633-640, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34950256

RESUMO

INTRODUCTION: Malignant esophageal obstruction is usually caused by esophageal and other chest cancers. More than 80% of cases of obstructive esophageal cancer (OEC) have lost the chance of curative resection. Stent insertion is a first-line palliative approach used to treat incurable OEC. AIM: To gauge the relative clinical efficacy of I-125 seed-loaded stent (ISS) versus normal stent (NS) insertion as a treatment for OEC. MATERIAL AND METHODS: Querying of the PubMed, Embase, and Cochrane Library databases was conducted to find all relevant studies published up to November 2020. The meta-analysis was undertaken using RevMan v5.3. RESULTS: We identified 158 studies initially, eight (4 randomized controlled trials and 4 retrospective studies) of which were used in this meta-analysis. We found that the two groups exhibited the comparable pooled Δdysphagia scores (MD = 0.02; p = 0.80), stent restenosis rates (OR = 0.97; p = 0.89), stent migration rates (OR = 0.81; p = 0.63), severe chest pain rates (OR = 1.05; p = 0.81), hemorrhage rates (OR = 1.53; p = 0.16), aspiration pneumonia rates (OR = 0.72; p = 0.38), and fistula formation rates (OR = 1.47; p = 0.44). The pooled time-to-restenosis and survival were both significantly longer in the ISS group (p = 0.04 and < 0.0001, respectively). Significant heterogeneity was detected in the endpoints of Δdysphagia scores and survival (I2 = 73% and 86%, respectively). Funnel plot analysis indicated an absence of publication bias related to the selected study endpoints. CONCLUSIONS: For patients with OEC, our meta-analysis indicated that ISS insertion could provide longer stent patency and survival than NS insertion.

18.
Front Oncol ; 11: 752642, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912709

RESUMO

BACKGROUND: Glucose-6-phosphate isomerase (GPI) plays an important role in glycolysis and gluconeogenesis. However, the role of GPI in lung adenocarcinoma (LUAD) remains unclear. METHODS: All original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and integrated via R 3.2.2. GPI expression was explored with TCGA, GEO, and Oncomine databases. Immunohistochemistry staining was used to analyze GPI expression in clinical specimens. The correlations between GPI and cancer immune characteristics were analyzed via the TIMER and TISIDB databases. GPI-specific siRNAs were used to verify the role of GPI expression on cell proliferation and cell cycle distribution. RESULTS: In general, GPI is predominantly overexpressed and has reference value in the diagnosis and prognostic estimation of LUAD. Upregulated GPI was associated with poorer overall survival, clinical stage, N stage, and primary therapy outcome in LUAD. Mechanistically, we identified a hub gene that included a total of 56 GPI-related genes, which were tightly associated with the cell cycle pathway in LUAD patients. Knockdown of GPI induced cell proliferation inhibition and cell cycle arrest. GPI expression was positively correlated with infiltrating levels of Th2 cells and regulatory T cells (Tregs); in contrast, GPI expression was negatively correlated with infiltrating levels of CD8+ T cells, central memory T cells, dendritic cells, macrophages, mast cells, and eosinophils. GPI was negatively correlated with the expression of immunostimulators, such as CD40L, IL6R, and TMEM173, in LUAD. CONCLUSION: GPI may play an important role in the cell cycle and can be used as a prognostic biomarker for determining the prognosis and immune infiltration in LUAD.

19.
Anal Chim Acta ; 1187: 339155, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34753577

RESUMO

A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nucleic acid aptamers. WGA was modified onto Fe3O4@Au magnetic nanoparticles (MNPs) for the efficient capture of S. aureus and L. mono in complex samples (orange juice, extracts of lettuce, and human urine) within 15 min. The streptavidin (SA)/aptamers co-functionalized SERS tags were fabricated by covalent attaching two different Raman reporters and SA molecules onto 45 nm Au NPs and then conjugated with two biotin-aptamers that specifically bind to their target bacteria with high affinity and stability. The combined use of high-sensitive SERS tags, WGA-mediated magnetic enrichment, and SA-mediated aptamer conjugation remarkably improved the assay sensitivity. Under optimized conditions, the developed SERS biosensor can simultaneously detect the two target bacteria with high detection sensitivity (<6 cells/mL), favorable linear relation (10-107 cells/mL), and high accuracy (recovery rate <7.03%). Therefore, the proposed SERS platform is rapid, sensitive, easy to use, and thus show potential as a tool for the timely identification of pathogenic bacteria in real samples.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Listeria monocytogenes , Nanopartículas Metálicas , Ouro , Humanos , Fenômenos Magnéticos , Análise Espectral Raman , Staphylococcus aureus , Estreptavidina , Aglutininas do Germe de Trigo
20.
Proc Natl Acad Sci U S A ; 118(47)2021 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-34799455

RESUMO

We demonstrate how two-photon excitation with quantum light can influence elementary photochemical events. The azobenzene trans → cis isomerization following entangled two-photon excitation is simulated using quantum nuclear wave packet dynamics. Photon entanglement modulates the nuclear wave packets by coherently controlling the transition pathways. The photochemical transition state during passage of the reactive conical intersection in azobenzene photoisomerization is strongly affected with a noticeable alteration of the product yield. Quantum entanglement thus provides a novel control knob for photochemical reactions. The distribution of the vibronic coherences during the conical intersection passage strongly depends on the shape of the initial wave packet created upon quantum light excitation. X-ray signals that can experimentally monitor this coherence are simulated.

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