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1.
BMC Oral Health ; 20(1): 55, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32066451

RESUMO

BACKGROUND: The aim of this study was to assess the difference of palatal morphology in different vertical patterns between skeletal Class I subjects and skeletal Class II subjects with retrusive mandible. METHODS: Seventy-six skeletal Class II subjects with retrusive mandible (38 females, 38 males) and 85 skeletal Class I subjects (45 females, 40 males) were collected retrospectively and divided into hyperdivergent, normodivergent and hypodivergent groups. CBCT images of these subjects were reoriented by Dolphin 3D Imaging software. Three-dimensional (3D) maxilla was segmented by ProPlan software before using Geomagic Studio software to reconstruct 3D palatal morphology. Deviation patterns on 3D colored map analysis was performed to compare the difference of 3D palatal morphology between different groups. RESULTS: 3D colored map analysis showed that male's palate was higher and wider than that of female in the posterior part, regardless of different sagittal and vertical patterns. In skeletal Class II subjects with retrusive mandible, males with hyperdivergent and normodivergent showed higher and narrower in the posterior part of palate, while females with hyperdivergent and normodivergent had a higher but no obviously narrow palate compared with the hypodivergent subjects. Skeletal Class II subjects with retrusive mandible showed flatter and narrower in the posterior part of palate than that of skeletal Class I subjects. CONCLUSIONS: Sagittal and vertical patterns have great influence on the palatal morphology and as the vertical dimension increased, the palate tended to be higher and narrower.

2.
Nat Immunol ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32034311

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

3.
Science ; 367(6478): 688-694, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32029629

RESUMO

Synapses between engram cells are believed to be substrates for memory storage, and the weakening or loss of these synapses leads to the forgetting of related memories. We found engulfment of synaptic components by microglia in the hippocampi of healthy adult mice. Depletion of microglia or inhibition of microglial phagocytosis prevented forgetting and the dissociation of engram cells. By introducing CD55 to inhibit complement pathways, specifically in engram cells, we further demonstrated that microglia regulated forgetting in a complement- and activity-dependent manner. Additionally, microglia were involved in both neurogenesis-related and neurogenesis-unrelated memory degradation. Together, our findings revealed complement-dependent synapse elimination by microglia as a mechanism underlying the forgetting of remote memories.

4.
Br J Cancer ; 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31919406

RESUMO

BACKGROUND: Chemoresistance remains a critical event that accounts for colorectal cancer (CRC) lethality. The aim of this study is to explore the ability of dichloroacetate (DCA) to increase chemosensitivity in CRC and the molecular mechanisms involved. METHODS: The effects of combination treatment of DCA and oxaliplatin (L-OHP) were analysed both in vitro and in vivo. The DCA-responsive proteins in AMPK pathway were enriched using proteomic profiling technology. The effect of DCA on CAB39-AMPK signal pathway was analysed. In addition, miRNA expression profiles after DCA treatment were determined. The DCA-responsive miRNAs that target CAB39 were assayed. Alterations of CAB39 and miR-107 expression were performed both in vitro and on xenograft models to identify miR-107 that targets CAB39-AMPK-mTOR signalling pathway. RESULTS: DCA increased L-OHP chemosensitivity both in vivo and in vitro. DCA could upregulate CAB39 expression, which activates the AMPK/mTOR signalling pathway. CAB39 was confirmed to be a direct target of miR-107 regulated by DCA. Alterations of miR-107 expression were correlated with chemoresistance development in CRC both in vitro and in vivo. CONCLUSION: These findings suggest that the miR-107 induces chemoresistance through CAB39-AMPK-mTOR pathway in CRC cells, thus providing a promising target for overcoming chemoresistance in CRC.

5.
Redox Biol ; 29: 101402, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31926626

RESUMO

Oxidative stress is a major cause of adverse outcomes in preeclampsia (PE). Ferroptosis, i.e. programmed cell death from iron-dependent lipid peroxidation, likely mediates PE pathogenesis. We evaluated specific markers for ferroptosis in normal and PE placental tissues, using in vitro (trophoblasts) and in vivo (rat) models. Increase in malondialdehyde content and total Fe2+ along with reduced the glutathione content and glutathione peroxidase activity was observed in PE placenta. While the trophoblasts experienced death under hypoxia, inhibitors of ferroptosis, apoptosis, autophagy, and necrosis increased the cell viability. Microarrays, bioinformatic analysis, and luciferase reporter assay revealed that upregulation of miR-30b-5p in PE models plays a pivotal role in ferroptosis, by downregulating Cys2/glutamate antiporter and PAX3 and decreasing ferroportin 1 (an iron exporter) expression, resulting in decreased GSH and increased labile Fe2+. Inhibition of miR-30b-5p expression and supplementation with ferroptosis inhibitors attenuated the PE symptoms in rat models, making miR-30b-5p a potential therapeutic target for PE.

6.
Nutrients ; 12(1)2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31906493

RESUMO

Food labels comprise a national health-intervention policy that informs consumers of food-product nutritional value. Previous evidence has indicated that, compared to a purely numeric guideline-daily-amount label, a traffic-light-inspired, color-coded label more effectively conveys the nutritional level and increases the selection of healthier products. Therefore, we used functional magnetic resonance imaging to assess the mechanism whereby traffic-light and guideline-daily-amount labels influence food-related decision-making. Forty-four female dieters (age, mean = 20.0, standard deviation = 1.45 years) were recruited to participate in a food-choice task; healthy or unhealthy food options were presented with color-coded traffic-light or purely numeric guideline-daily-amount labels, and the participants were asked to state their preference. We found that, compared with the guideline-daily-amount label, a salient, red traffic-light label potentially reduced unhealthy food-related decision-making and activated the superior medial frontal gyrus and the supplementary motor area, which are implicated in the execution of responses and motor inhibition. For the same stimulus contrast, we also found increased activation in the anterior cingulate cortex, which is associated with salient information monitoring. Finally, we found stronger functional connectivity between the anterior cingulate cortex and inhibitory regions (inferior and middle frontal gyri) under red-traffic-light than under guideline-daily-amount label conditions. These results suggest that traffic-light-inspired labels may be a more effective means of public-policy intervention than are numeric labels conveying guideline daily amounts.

7.
Artif Cells Nanomed Biotechnol ; 48(1): 425-434, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31916462

RESUMO

Abdominal wall defects are associated with abdominal wall surgery, infection and tumour resection. Polypropylene (PP) mesh, which has excellent mechanical strength, is currently the primary clinical repair material. In repairing the abdominal wall, the mesh can erode the bowel and cause other problems. Constructing a barrier that induces a weak inflammatory response and promotes rapid recovery of the peritoneum is important. We used electrospinning technology to construct a silk fibroin coating on the abdominal surface of a PP patch. A rat model was used to compare the inflammatory responses, regeneration of peritoneal tissue, and antiadhesion effects of electrospun regenerated silk fibroin (RSF) coatings, polycaprolactone (PCL) coatings, and noncoated PP meshes. The inflammatory responses, antiadhesion fractions, and areas of RSF and PCL were better than those of PP at 6 weeks. RSF was associated with complete peritoneal regeneration, in contrast to PCL. At 12 weeks, the structure of the PCL peritoneum was unstable, and the adhesion fraction and area were significantly higher than those of RSF. The intact peritoneum could not be effectively regenerated. The RSF group exhibited lower IL-6 levels than the PCL and PP groups but higher VEGF, IL-10 and TGF-ß levels, making RSF more conducive to the regeneration of peritoneal and abdominal wall tissues.

8.
Med Sci Monit ; 26: e921835, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31942867

RESUMO

BACKGROUND To study the risk predictors of intracranial asymptomatic small aneurysm rupture by electrocardiographic (ECG)-gated 4D-CT angiography (4D-CTA). MATERIAL AND METHODS A total of 168 patients with intracranial aneurysms <7 mm who underwent 4D-CTA examinations were retrospectively analyzed and divided into a ruptured group and an unruptured group. The original scanning data of all cases were reconstructed to obtain 20 groups of data in 5% time intervals in the cardiac cycle. After processing with 3D workstation software, 20 sets of images and dynamic images were achieved. The morphological characteristics and clinical features of the 2 groups of aneurysms were analyzed through univariate analysis, then logistic regression analysis was performed for the meaningful indicators. Finally, the optimum diagnostic cut-off point was calculated by ROC curve analysis. RESULTS Univariate analysis showed that sex, smoking history, vascular bifurcation point, pulsation point appearance, aneurysm height, aspect ratio (AR), and size ratio (SR) were significantly different (P<0.05) between the ruptured group and unruptured group. Multivariate regression analysis indicated that the pulsation points (OR=8.188, 95% CI: 3.984-17.198) and high SR values (OR=5.325, 95% CI: 1.503-18.867) were independent predictors of asymptomatic small aneurysm rupture. When the SR value was higher than 1.65, the area below the ROC curve was 0.723, the diagnostic sensitivity was 75%, and the specificity was 80%. CONCLUSIONS The occurrence of pulsation points and SR values >1.65 were independent predictors for the rupture of asymptomatic small intracranial aneurysms <7 mm in diameter.

9.
Reproduction ; 159(1): 59-71, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31705793

RESUMO

The invasion of maternal decidua by extravillous trophoblast (EVT) is essential for the establishment and maintenance of pregnancy, and abnormal trophoblast invasion could lead to placenta-associated pathologies including early pregnancy loss and preeclampsia. SEC5, a component of the exocyst complex, plays important roles in cell survival and migration, but its role in early pregnancy has not been reported. Thus, the present study was performed to explore the functions of SEC5 in trophoblast cells. The results showed that SEC5 expression in human placental villi at first trimester was significantly higher than it was at the third trimester, and it was abundantly localized in the cytotrophoblast (CTB) and the trophoblastic column. SEC5 knockdown was accompanied by reduced migration and invasion in HTR-8/SVneo cells. In addition, the expression and plasma membrane distribution of integrin ß1 was also decreased. Furthermore, shRNA-mediated knockdown of SEC5 inhibited the outgrowth of first trimester placental explants. SEC5 and InsP3R were colocalized in the cytoplasm of HTR-8/SVneo cells, and the cell-permeant calcium chelator BAPTA-AM could significantly inhibit HTR-8/SVneo cell invasion. The Ca2+ imaging results showed that the 10% fetal bovine serum-stimulated cytosolic calcium concentration ([Ca2+]c) was not only reduced by downregulated SEC5 but also was blocked by the InsP3R inhibitor. Furthermore, either the [Ca2+]c was buffered by BAPTA-AM or the knockdown of SEC5 disrupted HTR-8/SVneo cell F-actin stress fibers and caused cytoskeleton derangement. Taken together, our results suggest that SEC5 might be involved in regulating trophoblast cell migration and invasion through the integrin/Ca2+ signal pathway to induce cytoskeletal rearrangement.

10.
Oncogene ; 39(2): 469-485, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31597953

RESUMO

The development of chemoresistance remains a major challenge that accounts for colorectal cancer (CRC) lethality. Dichloroacetate (DCA) was originally used as a metabolic regulator in the treatment of metabolic diseases; here, DCA was assayed to identify the mechanisms underlying the chemoresistance of CRC. We found that DCA markedly enhanced chemosensitivity of CRC cells to fluorouracil (5-FU), and reduced the colony formation due to high levels of apoptosis. Using the microarray assay, we noted that miR-149-3p was involved in the chemoresistance of CRC, which was modulated by wild-type p53 after DCA treatment. In addition, PDK2 was identified as a direct target of miR-149-3p. Mechanistic analyses showed that overexpression of miR-149-3p enhanced 5-FU-induced apoptosis and reduced glucose metabolism, similar to the effects of PDK2 knockdown. In addition, overexpression of PDK2 partially reversed the inhibitory effect of miR-149-3p on glucose metabolism. Finally, both DCA treatment and miR-149-3p overexpression in 5-FU-resistant CRC cells were found to markedly sensitize the chemotherapeutic effect of 5-FU in vivo, and this effect was also validated in a small retrospective cohort of CRC patients. Taken together, we determined that the p53/miR-149-3p/PDK2 signaling pathway can potentially be targeted with DCA treatment to overcome chemoresistant CRC.

11.
J Nanosci Nanotechnol ; 20(3): 1605-1612, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492322

RESUMO

The present study focuses on the microstructural and bioactive properties evolution in selective laser melting (SLM) ß titanium alloys. We have applied cross-scan strategy for improving mechanical properties and lower elastic modulus of SLMed Ti-20Mg-5Ta alloys which has been shown to be altering the microstructure and refining the grain size. The cross-scan strategy can refine the microstructure and induce various deformation textures in contrast to the conventional scan strategy. The microstructures of Ti-20Mg-5Ta alloys indicate that the cross-scan strategy will yield the best mechanical properties and lower elastic modulus. The corrosion behavior of the Ti-20Mg-5Ta alloys was studied during immersion in an acellular simulated body fluid (SBF) at 37±0.50 °C for 28 days. Both the mechanical and bioactive properties showed that the novel Ti-20Mg-5Ta alloys should be ideal for bone implants.

12.
Huan Jing Ke Xue ; 41(1): 253-261, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854926

RESUMO

This study was carried out in the ozone (O3) and biological activated carbon (BAC) section of a drinking water plant to investigate the effects of O3 on microbial and effluent disinfection by-products (DBPs) in BAC during drinking water treatment. The water quality, dissolved organic matter (DOM) characteristics, microbial activity, and DBPs formation at different O3 concentrations were analyzed. Results showed that the effect of O3 on microorganisms is mainly that it increased the utilization efficiency of DOM. However, excessive O3 increased the amount of organic matter such as protein and microbial metabolites (SMPs) in the effluent. When the O3 concentration increased from 0 mg·L-1 to 2.0 mg·L-1, the survival rate of microorganisms in the BAC decreased from 95.10% to 62.60%. However, since O3 transforms organic matter into a biodegradable substance, we found that microbial activity increased by 62.52% and that the biofiltration of the BAC was enhanced. When the O3 concentration was further increased to 4.0 mg·L-1, the microbial survival rate decreased to 49.9% and the protein and SMPs produced by the microorganisms increased. This resulted in an increased formation of carbon-containing DBPs (C-DBPs) and nitrogen-containing DBPs (N-DBPs) by 41.93% and 7.18%, respectively. In summary, an appropriate dosage of O3 was beneficial for removing DOM by O3-BAC, but we found that an excessive O3 concentration caused the formation of new DBPs precursors.


Assuntos
Carvão Vegetal , Água Potável/microbiologia , Ozônio , Purificação da Água/métodos , Desinfecção/métodos , Poluentes Químicos da Água , Qualidade da Água
13.
ACS Appl Mater Interfaces ; 11(51): 47720-47729, 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31793283

RESUMO

Regulating cell behavior and cell fate are of great significance for basic biological research and cell therapy. Carbohydrates, as the key biomacromolecules, play a crucial role in regulating cell behavior. Herein, "modular" glycopolymers were synthesized by reversible addition-fragmentation chain transfer polymerization. These glycopolymers contain sugar units (glucose), anchoring units (cholesterol), "guest" units (adamantane) for host-guest interaction, and fluorescent labeling units (fluorescein). It was demonstrated that these glycopolymers can insert into cell membranes with high efficiency and their residence time on the membranes can be regulated by controlling their cholesterol content. Furthermore, the behavior of the engineered cells can be controlled by modifying with different functional ß-cyclodextrins (CD-X) via host-guest interactions with the adamantane units. Host-guest interactions with the modular polymers were demonstrated using CD-RBITC (X = a rhodamine B isothiocyanate). The glycopolymers were modified with CD-S (X = seven sulfonate groups) and CD-M (X = seven mannose groups) and were then attached, respectively, to the surfaces of mouse embryonic stem cells for the promotion of neural differentiation and to the surfaces of cancer cells for the enhancement of the immune response. The combination of multiple anchors and host-guest interactions provides a widely applicable cell membrane modification platform for a variety of applications.

14.
Nanomaterials (Basel) ; 9(12)2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31795272

RESUMO

A comprehensive insight into the electronic and optical properties of small-lattice-mismatched InSe-GeTe heterobilayer (HBL) is performed based on the density functional theory (DFT) with van der Waals corrections from first-principles perspective. The optimization of most stable geometric stacking mode for the InSe-GeTe HBL is demonstrated. In addition, it is found that the InSe-GeTe HBL forms a type-II heterostructure of staggered-gap band alignment, resulting in an indirect band gap of 0.78 eV, which could be employed as a separator for electron-hole pairs. Moreover, the influence of biaxial strain on the electronic and optical properties of the InSe-GeTe HBL are systematically explored by calculating the band structures, density of states (PDOS), electron density differences, and optical absorption spectra of InSe-GeTe HBL under compressive and tensile biaxial strains. The results indicate that the electronic structures and optical performance of InSe-GeTe HBL could be modulated by changing the biaxial strain conveniently. Our findings provide new opportunities for the novel InSe-GeTe HBL to be applied in the electronic and optoelectronic fields.

15.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 1876-1880, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839053

RESUMO

OBJECTIVE: To investigate the expression level of T lymphocyte subsets in elderly patients with newly diagnosed multiple myeloma (NDMM), and to evaluated the prognostic value of T lymphocytic abnormalities in elderly NDMM patients. METHODS: Pretreated peripheral blood of 39 newly diagnosed elder patients with MM was tested by multi-parameter flow cytometry (MFC) to quantitatively detect T lymphocyte subsets, including CD4+T cell, CD8+T cell, and CD4/CD8 ratio. The prognostic values T-lymphocyte subset were evaluated in newly diagnosed elderly patients with MM. RESULTS: The median follow-up time was 21.5 (range, 3.0-66.0) months. Absolute counts of CD4+T cell and CD4/CD8 ratio positively correlated with prognosis. In the multivariate COX analysis, lower CD4/CD8 ratio and CD4+T cell counts were identified to be independent adverse prognostic factors for OS. CONCLUSION: Lower CD4/CD8 ratio and CD4+T cell counts at initial diagnosis are independent unfavorable prognostic factors for elderly patients with MM, and T lymphocyte subsets are crucial indicators for MM patients' prognosis.


Assuntos
Mieloma Múltiplo , Idoso , Relação CD4-CD8 , Citometria de Fluxo , Humanos , Contagem de Linfócitos , Subpopulações de Linfócitos , Prognóstico , Subpopulações de Linfócitos T
16.
Sci Rep ; 9(1): 19149, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31844132

RESUMO

Terpenes and their derivatives are sustainable, renewable chemicals that can be used as a complementary hydrocarbon. The exceptions are fossil-based feedstocks and lignin-based feedstocks. A simple method has been found to prepare allyl terpene maleate monomer by substitution reaction at lower reaction temperatures. Using terpenes from turpentine, maleic anhydride and allyl chloride as reactants, the synthesized monomer, terpene-diallyl maleate adduct, was prepared by D-A addition, hydrolysis, and substitution reaction. The resultant monomer was characterized for the first time. The synthesized product will be a versatile monomer and a very important intermediate, having broad application prospects. The synthesized monomer will replace similar aromatic compounds in certain applications because of its low-toxicity and sustainability. The synthesized monomer with two terminal olefin structures has great free radical polymerization potential, according to its physical and chemical properties and exploratory experimentation.

17.
Cogn Sci ; 43(12): e12804, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31858627

RESUMO

The temporal-focus hypothesis claims that whether people conceptualize the past or the future as in front of them depends on their cultural attitudes toward time; such conceptualizations can be independent from the space-time metaphors expressed through language. In this paper, we study how Chinese people conceptualize time on the sagittal axis to find out the respective influences of language and culture on mental space-time mappings. An examination of Mandarin speakers' co-speech gestures shows that some Chinese spontaneously perform past-in-front/future-at-back (besides future-in-front/past-at-back) gestures, especially when gestures are accompanying past-in-front/future-at-back space-time metaphors (Exp. 1). Using a temporal performance task, the study confirms that Chinese can conceptualize the future as behind and the past as in front of them, and that such space-time mappings are affected by the different expressions of Mandarin space-time metaphors (Exp. 2). Additionally, a survey on cultural attitudes toward time shows that Chinese tend to focus slightly more on the future than on the past (Exp. 3). Within the Chinese sample, we did not find evidence for the effect of participants' cultural temporal attitudes on space-time mappings, but a cross-cultural comparison of space-time mappings between Chinese, Moroccans, and Spaniards provides strong support for the temporal-focus hypothesis. Furthermore, the results of Exp. 2 are replicated even after controlling for factors such as cultural temporal attitudes and age (Exp. 3), which implies that linguistic sagittal temporal metaphors can indeed influence Mandarin speakers' space-time mappings. The findings not only contribute to a better understanding of Chinese people's sagittal temporal orientation, but also have additional implications for theories on the mental space-time mappings and the relationship between language and thought.

18.
J Clin Lab Anal ; : e23143, 2019 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-31867757

RESUMO

OBJECTIVE: The aim of this study was to establish a risk scoring system for the decreased glomerular filtration rate (GFR) in Chinese general population. METHODS: Totally 781 participants who underwent a health checkup in The First Affiliated Hospital of Nanjing Medical University from January to September 2017 were involved in the study. Significant variables chosen by multivariable logistic regression analysis were allocated the integral scores in proportion to its odds ratio (OR), and then the risk of decreased GFR was assessed based on the scores. RESULTS: The people with abnormal homocysteine (Hcy) level (OR: 1.534, 95% confidential interval [CI]: 1.075-2.190, P = .018), males (OR: 2.054, 95%CI: 1.365-3.092, P < .001), and those at the age of 46-52 years (OR: 2.943, 95%CI: 1.546-5.605, P = .001), 52-59 years (OR: 3.664, 95%CI: 1.937-6.931, P < .001) and ≥59 years (OR: 13.452, 95%CI: 7.339-24.657, P < .001) were subjected to GFR reduction. These three variables were allocated the integral scores in proportion to its OR, and four risk categories were divided according to the scores. The prevalence of the decreased GFR in people with low (score 0-4, n = 8), below the average (score 4-6, n = 37), above the average (score 6-13, n = 47), and high risks (score ≥ 13, n = 103) was 5.26%, 16.89%, 22.93% and 50.24%, respectively, and this prevalence raised with the increase of scores (P < .001). CONCLUSIONS: A risk scoring system is developed in this study, which may offer a specific risk stratification for GFR reduction in Chinese general population.

19.
Ther Adv Respir Dis ; 13: 1753466619890538, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31771432

RESUMO

Autophagy is a process of cell self-renewal that is dependent on the degradation of the cytoplasmic proteins or organelles of lysosomes. Many diseases, such as metabolic diseases, cancer, neurodegenerative diseases, and lung diseases, have been confirmed to be associated with elevated or impaired levels of autophagy. At present, studies have found that autophagy participates in the regulation of chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis, pulmonary hypertension, acute lung injury, lung cancer, and other pulmonary diseases. Using recent literature on the signal transduction mechanisms of autophagy and the effects of autophagy signalling on lung diseases, this review intends to clarify the mechanisms of lung disease to guide the treatment of related diseases. The reviews of this paper are available via the supplemental material section.

20.
Exp Ther Med ; 18(6): 4356-4362, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31772631

RESUMO

Atherosclerosis (AS) is the leading cause of cardiovascular disease and poses a threat to human health. MicroRNAs (miRNAs/miRs) are a group of endogenous small non-coding RNAs that have been identified to serve important roles in AS. However, the expression and role of miR-133a-3p in AS remains unclear. The aim of the present study was to investigate miR-133a-3p in AS and to determine its underlying mechanism. The level of miR-133a-3p expression in the blood and vascular plaque tissue of patients with AS was detected via reverse transcription-quantitative PCR (RT-qPCR). The role of miR-133a-3p in human vascular smooth muscle cells (hVSMCs) was investigated, following upregulation and downregulation of this miR in hVSMCs. Cell proliferation and apoptosis were determined using a Cell Counting kit-8 assay and flow cytometry, respectively. The results demonstrated the downregulation of miR-133a-3p in the blood and vascular plaque tissue of patients with AS. Matrix metallopeptidase-9 (MMP-9) was revealed to be a direct target gene of miR-133a-3p, which was upregulated in the blood and vascular plaque tissue of patients with AS. Furthermore, MMP-9 was determined to be negatively regulated by miR-133a-3p in hVSMCs. In addition, significant inhibition of hVSMC proliferation and induction of cell apoptosis were observed following MMP-9 downregulation and following transfection with the miR-133a-3p mimic. The effects of the miR-133a-3p mimic on hVSMC proliferation and apoptosis were reversed by MMP-9 over-expression. Overall, the results indicated that miR-133a-3p was downregulated in AS, which results in the inhibition of hVSMC proliferation and the induction of cell apoptosis via MMP-9. miR-133a-3p may therefore be a promising therapeutic target for the treatment of AS.

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