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1.
Sci Total Environ ; : 151400, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34742802

RESUMO

In grasslands, roots of different plant species decay in combination in the presence of living plants, besides, most root decomposition studies are conducted on how roots of plants decomposed alone or in artificial compositions in the absence of living plants. Therefore, we evaluated how roots of different perennial plants induced effects on decomposition process under living plants and their associated mechanisms. By using litter bag technique, we determined the root decomposition process of three perennial plants, Leymus chinensis, Phragmites australis, and Kalimeris integrifolia grown in monocultures, bi- and tri-species mixtures, after 12 months of incubation under living plants and bare soil communities. We found both additive and non-additive effects on decomposition dynamics indicating that root mass losses of compositions cannot be calculated from decaying rates of individual species. The rich-nutrient roots of K. integrifolia in monocultures and in mixtures with other plant species decayed faster. Compared with bare soil, microbial activities were enhanced under living plant communities and hence stimulated decomposition rates. Our results indicated that microbial activities are important but secondary factors to root physico-chemical properties impacting root decomposition rates. In conclusion, the empirical relationships developed here are helpful to better understand the effects of root properties and microbial activities on decay rates.

2.
Exp Brain Res ; 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34661743

RESUMO

This study aimed to establish the role of miR-129 and miR-384-5p in cerebral ischemia-induced apoptosis. Using PC12 cells transfected with miR-129 or miR-384-5p mimics or inhibitors, oxygen glucose deprivation (OGD) conditions were applied for 4 h to simulate transient cerebral ischemia. Apoptotic phenotypes were assessed via lactate dehydrogenase (LDH) assay, MTT cell metabolism assay, and fluorescence-activated cell sorting (FACS). The effect of miR overexpression and inhibition was evaluated by protein and mRNA detection of bcl-2 and caspase-3, critical apoptosis factors. Finally, the direct relationship of miR-129 and bcl-2 and miR-384-5p and caspase-3 was measured by luciferase reporter assay. The overexpression of miR-384-5p and miR-129 deficiency significantly enhanced cell viability, reduced LDH release, and inhibited apoptosis. By contrast, overexpression of miR-129 and miR-384-5p deficiency aggravated hypoxia-induced apoptosis and cell injury. miR-129 overexpression significantly reduced mRNA and protein levels of bcl-2 and miR-129 inhibition significantly increased mRNA and protein levels of bcl-2 in hypoxic cells.miR-384-5p overexpression significantly reduced protein levels of caspase-3 while miR-384-5p deficiency significantly increased protein levels of caspase-3. However, no changes were observed in caspase-3 mRNA in either transfection paradigm. Finally, luciferase reporter assay confirmed caspase-3 to be a direct target of miR-384-5p; however, no binding activity was detected between bcl-2 and miR-129.Transient cerebral ischemia induces differential expression of miR-129 and miR-384-5p which influences apoptosis by regulating apoptotic factors caspase-3 and bcl-2, thereby participating in the pathological mechanism of cerebral ischemia, and becoming potential targets for the treatment of ischemic cerebral injury in the future.

3.
Colloids Surf B Biointerfaces ; 208: 112069, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34478957

RESUMO

Antimicrobial peptides/DNA complexes were designed based on AMPs chensinin-1b and its corresponding lipo-chensinin-1b conjugated with an aliphatic acid with different chain lengths and therapeutic genes. The main goal of such a complex includes two aspects: first, antimicrobial peptides deliver therapeutic genes to cancer cells and genes expressed in targeted tissue for cancer gene therapy, and, second, the antimicrobial peptide kills cancer cells when used alone as an anticancer agent. This study presents a model composed of chensinin-1b and its lipo-chensinin-1b and eGFP plasmids, which were used as reporter genes, and the final peptide/eGFP plasmid complexes were analyzed by TEM and DLS. The gene transfection efficiency of the complex was evaluated by a microplate reader, FACS and CLSM. Compared with Lipo2000, the antimicrobial peptide showed specific selectivity for transfection against cancer cells and mammalian cells. The peptides chensinin-1b and lipo-chensinin-1b binding with the eGFP plasmid displayed optimal transfection efficiencies at a mass ratio of 8. In addition, PA-C1b can deliver p53-eGFP plasmids into MCF-7 cancer cells, and the proliferation of cells was inhibited and even caused cell death. Overall, PA-C1b was screened and found to have the highest transfection efficiency for gene delivery and good cellular uptake capability. The in vivo transfection ability of PA-C1b was investigated using a tumor-bearing mouse model, and the transfection efficiency reflected by the fluorescence of expressed GFP was determined by in vivo imaging. Conclusively, the antimicrobial peptide PA-C1b could be used as the nonviral vector with high efficiency for cancer gene therapy.


Assuntos
Técnicas de Transferência de Genes , Neoplasias , Acilação , Animais , DNA/genética , Camundongos , Neoplasias/genética , Neoplasias/terapia , Plasmídeos/genética , Proteínas Citotóxicas Formadoras de Poros
4.
Sci Rep ; 11(1): 18347, 2021 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-34526604

RESUMO

To investigate the ability of CT-based radiomics signature for pre-and postoperatively predicting the early recurrence of intrahepatic mass-forming cholangiocarcinoma (IMCC) and develop radiomics-based prediction models. Institutional review board approved this study. Clinicopathological characteristics, contrast-enhanced CT images, and radiomics features of 125 IMCC patients (35 with early recurrence and 90 with non-early recurrence) were retrospectively reviewed. In the training set of 92 patients, preoperative model, pathological model, and combined model were developed by multivariate logistic regression analysis to predict the early recurrence (≤ 6 months) of IMCC, and the prediction performance of different models were compared using the Delong test. The developed models were validated by assessing their prediction performance in test set of 33 patients. Multivariate logistic regression analysis identified solitary, differentiation, energy- arterial phase (AP), inertia-AP, and percentile50th-portal venous phase (PV) to construct combined model for predicting early recurrence of IMCC [the area under the curve (AUC) = 0.917; 95% CI 0.840-0.965]. While the AUC of pathological model and preoperative model were 0.741 (95% CI 0.637-0.828) and 0.844 (95% CI 0.751-0.912), respectively. The AUC of the combined model was significantly higher than that of the preoperative model (p = 0.049) or pathological model (p = 0.002) in training set. In test set, the combined model also showed higher prediction performance. CT-based radiomics signature is a powerful predictor for early recurrence of IMCC. Preoperative model (constructed with homogeneity-AP and standard deviation-AP) and combined model (constructed with solitary, differentiation, energy-AP, inertia-AP, and percentile50th-PV) can improve the accuracy for pre-and postoperatively predicting the early recurrence of IMCC.

5.
Semin Dial ; 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34585446

RESUMO

OBJECTIVE: To evaluate the efficacy and safety of nine pharmacological interventions for restless legs syndrome (RLS) in dialysis patients. METHODS: An electronic database was used to retrieve eligible trials from PubMed, Cochrane, Embase, and Web of Science. Stata 14.2 software was used to perform network meta-analysis. The primary measure was the RLS score, and the secondary measure was used to evaluate the side effects of the drug. The surface under the cumulative ranking curve method was used to rank the merits of intervention measures. A comparison of the two interventions is shown on a league table. RESULTS: Finally, nine randomized controlled trials (RCTs) with a total of 377 participants were included. From the results of the network meta-analysis, all treatments ranked higher than placebo in terms of improving clinical symptoms, but only vitamin C (standardized mean difference [SMD] = -1.47 95% confidence interval [CI] -2.89, -0.05) showed significant differences compared with placebo. In terms of safety, there were no serious adverse reactions to any of the treatments compared to placebo. CONCLUSION: Currently, existing evidence suggests that vitamin C may be the most ideal drug to improve the symptoms of RLS in dialysis patients.

6.
Br J Clin Pharmacol ; 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34214221

RESUMO

Acute pancreatitis refers to pancreatic enzyme activation caused by a variety of aetiologies, and mainly characterized by local inflammation of the pancreas, with or without diseases of other organ function changes. The main clinical features are abdominal pain and elevated trypsin levels in the blood. Common causes of acute pancreatitis include cholelithiasis, alcohol consumption and hyperlipidaemia, among which drugs are considered to be one of the rare causes of pancreatitis. The patient in this case was a 16-year-old adolescent female who developed acute severe pancreatitis during the treatment of cryptococcal meningitis with voriconazole for 35 days. Following diagnosis that pancreatitis was induced by voriconazole, the drug was immediately stopped and the patient was discharged after symptomatic treatment. The phenomenon of voriconazole-induced pancreatitis is extremely rare, but we hope that this report can arouse greater attention and vigilance of the majority of medical personnel to improve the safety of patients' medication, especially for children or minors.

7.
Bioelectrochemistry ; 141: 107846, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34087545

RESUMO

Early finding and diagnosis are critical for prevention and treatment of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a typical biomarker of HCC. Since AFP level can reflect the severity of HCC, it is essential to ensure the accurate detection of AFP. In this study, through a combination of the advantages exhibited by ordered mesoporous carbon (OMC)@gold nanoparticles (AuNPs) composites and AuPt-methylene blue (AuPt-MB), a disposable ultrasensitive sandwich-configuration electrochemical immunosensor for determination of AFP was designed. Characterized by excellent conductivity, highly ordered pore distribution and great surface area, OMC can be effective in promoting electron transfer and loading a large number of AuNPs. In the meantime, AuNPs can also immobilize AFP-Ab1 through Au-N bonds. As a new redox-active species, rod-like AuPt-MB demonstrates high conductivity, uniform morphology and excellent biocompatibility, which makes it capable not only to fix AFP-Ab2, but also to release electrochemical signals. A wide linearity of 10 fg mL-1-100 ng mL-1 and a low detection limit of 3.33 fg mL-1 (S/N = 3) were obtained. Moreover, the proposed immunosensor exhibited acceptable selectivity, high stability and reproducibility. The excellent performance in detecting serum samples endows the proposed immunosensor with broad prospects of extensive application in the detection of disease biomarkers.

8.
Oxid Med Cell Longev ; 2021: 8840896, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34007406

RESUMO

Salvia miltiorrhiza (SM) coupled with Dalbergia odorifera (DO) has been used to relieve cardiovascular diseases in China for many years. Our previous studies have integrated that SM-the volatile oil of DO (SM-DOO)-has a cardioprotective effect on chronic myocardial ischemia based on a pharmacological method, but the cardioprotective mechanism has not been elucidated completely in the metabonomic method. In the present study, a metabonomic method based on high-performance liquid chromatography time-of-flight mass spectrometry (HPLC-Q-TOF-MS) was performed to evaluate the effects of SM-DOO on chronic myocardial ischemia induced by an ameroid constrictor, which was placed on the left anterior descending coronary artery (LAD) of pigs. Pigs were divided into three groups: sham, model, and SM-DOO group. With multivariate analysis, a clear cluster among the different groups was obtained and the potential biomarkers were recognized. These biomarkers were mainly related to energy metabolism, glucose metabolism, and fatty acid metabolism. Furthermore, the protein expressions of phosphorylated AMP-activated protein kinase (p-AMPK) and glucose transporter-4 (GLUT4) were significantly upregulated by SM-DOO. The result indicated that SM-DOO could regulate the above biomarkers and metabolic pathways, especially energy metabolism and glucose metabolism. By analyzing and verifying the biomarkers and metabolic pathways, further understanding of the cardioprotective effect of SM-DOO with its mechanism was evaluated. Metabonomic is a reliable system biology approach for understanding the cardioprotective effects of SM-DOO on chronic myocardial ischemia and elucidating the mechanism underlying this protective effect.


Assuntos
Dalbergia/química , Metabolômica/métodos , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Salvia miltiorrhiza/química , Animais , Modelos Animais de Doenças , Masculino , Suínos
9.
Ann Transl Med ; 9(8): 664, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33987362

RESUMO

Background: LncRNAs play an important regulatory function in the occurrence and progression of papillary thyroid cancer (PTC). This study aimed to investigate the role and mechanism of ATP binding cassette subfamily C member 6 pseudogene 1 (ABCC6P1) in PTC. Methods: Cancerous and paracancer normal thyroid tissues were collected from 18 patients with PTC, who were operated at the Second Affiliated Hospital of Harbin Medical University. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to investigate the levels of ABCC6P1. Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8) and colony formation assays. Wound healing and Transwell invasion assays were performed to examine cell migratory and invasive ability. Western blotting analysis was used to detect the expression levels of EMT-related markers and Wnt/ß-catenin signaling pathway-related proteins. Results: The expression of ABCC6P1 was upregulated in PTC tissues and cells. ABCC6P1 silencing could significantly suppress the proliferation, colony formation ability, migratory and invasive ability in PTC cells. Moreover, knockdown of ABCC6P1 induced cell cycle arrest at G0/G1 phase and inhibited epithelial-mesenchymal transition (EMT) process of PTC cells by increasing the E-cadherin expression, but downregulating N-cadherin and vimentin expression. In addition, knockdown of ABCC6P1 caused a significant decrease in levels of Wnt/ß-catenin signaling pathway members (including ß-catenin, c-myc, and cyclin D1) in PTC cells. Conclusions: Our study confirms that ABCC6P1 exerts an oncogenic activity in PTC which may be mediated by the Wnt/ß-catenin pathway, suggesting that ABCC6P1 may be a promising therapeutic target for PTC.

10.
Front Physiol ; 12: 639454, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33841179

RESUMO

Background and Aims: Previous studies have demonstrated the anti-hypertensive effect of chronic intermittent hypobaric hypoxia (CIHH) in hypertensive rats. The present study investigated the anti-hypertensive effect of CIHH in spontaneously hypertensive rats (SHR) and the role of the renin-angiotensin system (RAS) in anti-hypertensive effect of CIHH. Methods: Fifteen-week-old male SHR and WKY rats were divided into four groups: the SHR without CIHH treatment (SHR-CON), the SHR with CIHH treatment (SHR-CIHH), the WKY without CIHH treatment (WKY-CON), and the WKY with CIHH treatment (WKY-CIHH) groups. The SHR-CIHH and WKY-CIHH rats underwent 35-days of hypobaric hypoxia simulating an altitude of 4,000 m, 5 h per day. Arterial blood pressure and heart rate were recorded by biotelemetry, and angiotensin (Ang) II, Ang1-7, interleukin (IL)-6, tumor necrosis factor-alpha (TNF)-α, and IL-10 in serum and the mesenteric arteries were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. The microvessel tension recording technique was used to determine the contraction and relaxation of the mesenteric arteries. Hematoxylin and eosin and Masson's staining were used to observe vascular morphology and fibrosis. Western blot was employed to detect the expression of the angiotensin-converting enzyme (ACE), ACE2, AT1, and Mas proteins in the mesenteric artery. Results: The biotelemetry result showed that CIHH decreased arterial blood pressure in SHR for 3-4 weeks (P < 0.01). The ELISA and immunohistochemistry results showed that CIHH decreased Ang II, but increased Ang1-7 in serum and the mesenteric arteries of SHR. In the CIHH-treated SHR, IL-6 and TNF-α decreased in serum and the mesenteric arteries, and IL-10 increased in serum (P < 0.05-0.01). The microvessel tension results revealed that CIHH inhibited vascular contraction with decreased Ang1-7 in the mesenteric arteries of SHR (P < 0.05-0.01). The staining results revealed that CIHH significantly improved vascular remodeling and fibrosis in SHR. The western blot results demonstrated that CIHH upregulated expression of the ACE2 and Mas proteins, and downregulated expression of the ACE and AT1 proteins (P < 0.05-0.01). Conclusion: CIHH decreased high blood pressure in SHR, possibly by inhibiting RAS activity, downregulating the ACE-Ang II-AT1 axis and upregulating the ACE2-(Ang1-7)-Mas axis, which resulted in antagonized vascular remodeling and fibrosis, reduced inflammation, and enhanced vascular relaxation.

11.
Life Sci ; 278: 119540, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-33930369

RESUMO

AIM: The aim of our study was to clarify the cardioprotection of chronic intermittent hypobaric hypoxia (CIHH) and the underlying mechanism in spontaneously hypertensive rats (SHR). MAIN METHODS: Adult male rats were divided into normal blood pressure Wistar-Kyoto rats (WKY) control (WKY-CON), WKY rats with CIHH treatment (WKY-CIHH), SHR control (SHR-CON) and SHR with CIHH treatment (SHR-CIHH) groups. SHR-CIHH and WKY-CIHH rats were subjected to hypobaric hypoxia simulating 4000-m altitude for 35 days, 5 h per day. Arterial blood pressure and cardiac function parameters, including ejection fraction, fractional shortening and left ventricular (LV) wall thickness, were evaluated. Cardiac pathomorphology and myocardial fibrosis were determined. The expression of angiotensin-converting enzyme (ACE), ACE2, Ang II, Ang1-7, AT1 receptor, Mas receptor, IL-6, TNF-α,IL-10, SOD and MDA were assayed in myocardium. KEY FINDINGS: CIHH significantly decreased arterial blood pressure, alleviated LV hypertrophy, and improved cardiovascular function in SHR (P < 0.05-0.01). Also, CIHH protected SHR heart against morphological changes and fibrosis. In addition, CIHH significantly down-regulated the ACE/Ang II/AT1 receptor axis and up-regulated the ACE2/Ang1-7/Mas axis of renin-angiotensin system (RAS) in SHR (P < 0.05-0.01). CIHH significantly reduced IL-6, TNF-α, and MDA levels, but increased IL-10 and SOD in SHR myocardium (P < 0.05-0.01). SIGNIFICANCE: The CIHH treatment protected the heart of SHR against LV remodelling and myocardial fibrosis, which might be carried out through a balance in the ACE/Ang II/AT1 axis and the ACE2/Ang1-7/Mas axis of the RAS to reduce inflammation, and inhibit oxidative stress.


Assuntos
Doença da Altitude/epidemiologia , Hipertensão/epidemiologia , Hipertensão/patologia , Miocárdio/patologia , Sistema Renina-Angiotensina , Remodelação Ventricular , Altitude , Animais , Fibrose , Hipertensão/metabolismo , Masculino , Miocárdio/metabolismo , Oxigênio/metabolismo , Fatores de Proteção , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY
12.
Zhongguo Gu Shang ; 34(1): 63-7, 2021 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-33666022

RESUMO

OBJECTIVE: To compare the clinical outcomes of hydraulic perfusion pump and traditional water fhushing in percutaneous endoscopic lumbar discectomy. METHODS: From January 2016 to December 2018, 72 patients with lumbar disc herniation failed to conservative treatment were enrolled in this study. The patients were divided into hydraulic perfusion pump group and traditional water flushing group, 36 cases in each group. There were no significant differences in gender, age, prominent segment, clinical classification, preoperative visual analogue scale (VAS) and Japanese Orthopaedic Association(JOA) score between two groups (P>0.05). All operations were performed by the same surgeon, and the operations were completed by hydraulic perfusion pumpflushing or traditional water flushing assisted surgery. The operation time, intraoperative blood loss, hospital stay were recorded;VAS and JOA scores were observed before and after operation;the incidences of postoperative complications were compared between two groups. RESULTS: All the patients were followed up for 12 to 24 (15.7±5.1) months. Compared with the traditional water flushing group, the operation time of the hydraulic perfusion pump group was shorter [(65.5±21.3) min vs (74.8±19.9) min, t=9.465, P=0.003], and intraoperative blood loss was less [(21.2±12.9)ml vs (27.4± 14.1) ml, t=8.331, P=0.012], there was no statistical difference in hospital stay between two groups. Postoperative VAS scores at 1 day, 2 weeks and the final follow-up and postoperative JOA scores at the final follow-up of the two groups of patients improved compared with those before operation, but there was no significant difference between two groups (P>0.05). In the traditional water flushing group, the intracranial hypertensionoccurred during operation and the operation time was forced to shorten in 5 cases, the nerve root adventitia injury in 4 cases, and the postoperative recurrence in 4 cases. No intracranial hypertension during operation was found in the hydraulic perfusion pump group, nerve root adventitia injury occurred in 1 case, postoperative recurrence in 2 cases. The incidence of complication of the hydraulic perfusion pump group was lower than that ofthe traditional water flushing group. CONCLUSION: Both hydraulic perfusion pump and traditional water flushing assisted percutaneous endoscopic lumbar disc herniation can achieve satisfactory clinical results, but the former has shorter operation time, clearer intraoperative vision, less bleeding, and fewer intraoperative and postoperative complications.


Assuntos
Discotomia Percutânea , Deslocamento do Disco Intervertebral , Discotomia , Endoscopia , Humanos , Bombas de Infusão , Deslocamento do Disco Intervertebral/cirurgia , Vértebras Lombares/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Água
13.
Zhongguo Zhong Yao Za Zhi ; 46(5): 1128-1133, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33787106

RESUMO

On the basis of the previous work of the research group, the orthogonal design method was further used to optimize the processing technology for reducing toxicity of fried Tripterygium wilfordii in Lysimachia christinae Decoction. A total of 9 processed products of T.wilfordii in L.christinae decoction were prepared by four factors and three levels orthogonal design table. The contents of triptolide in T.wilfordii were determined by high performance liquid chromatography(HPLC) before and after processing: 4.27, 3.92, 3.57, 2.75, 2.42, 2.66, 3.51, 1.87, 1.75, 2.03 µg·g~(-1). On this basis, the above processed products were orally given to mice for 28 days. 12 hours after the last administration, food fasting except water was provided, and 24 hours later, the eyeballs were taken for blood and liver tissue. Serum biochemical indexes, liver lipid peroxidation and antioxidant related indexes were detected by kit method. Twenty-eight days after oral administration of raw T.wilfordii, the levels of serum alanine aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and liver malondialdehyde(MDA) in mice increased by 91%(P<0.01), 46%(P<0.05), 73%(P<0.01) and 99%(P<0.01), while the liver antioxidant indexes such as superoxide dismutase(SOD), glutathione(GSH), glutathione peroxidase(GPX) and glutathione-S transferase(GST) significantly decreased(P<0.01). After administration of the processed products, the above indexes were significantly reversed(P<0.01 or P<0.05). Especially, the processing conditions of A_3B_2C_1D_3 had the best detoxification effect on T.wilfordii, which decreased the high levels of AST, ALT, ALP and MDA by 49%(P<0.01), 32%(P<0.01), 42%(P<0.01), and 17%(P<0.05). Therefore, the best processing conditions for T.wilfordii in L.christinae decoction were A_3B_2C_1D_3, namely "15% mass fraction of L.christinae, 1 h moistening time, 160 ℃ frying temperature, and 9 min frying time".


Assuntos
Primulaceae , Tripterygium , Animais , Antioxidantes , Fígado , Camundongos , Tecnologia
14.
Sci Rep ; 11(1): 6933, 2021 03 25.
Artigo em Inglês | MEDLINE | ID: mdl-33767315

RESUMO

To explore the value of contrast-enhanced CT texture analysis in predicting isocitrate dehydrogenase (IDH) mutation status of intrahepatic cholangiocarcinomas (ICCs). Institutional review board approved this study. Contrast-enhanced CT images of 138 ICC patients (21 with IDH mutation and 117 without IDH mutation) were retrospectively reviewed. Texture analysis was performed for each lesion and compared between ICCs with and without IDH mutation. All textural features in each phase and combinations of textural features (p < 0.05) by Mann-Whitney U tests were separately used to train multiple support vector machine (SVM) classifiers. The classification generalizability and performance were evaluated using a tenfold cross-validation scheme. Among plain, arterial phase (AP), portal venous phase (VP), equilibrium phase (EP) and Sig classifiers, VP classifier showed the highest accuracy of 0.863 (sensitivity, 0.727; specificity, 0.885), with a mean area under the receiver operating characteristic curve of 0.813 in predicting IDH mutation in validation cohort. Texture features of CT images in portal venous phase could predict IDH mutation status of ICCs with SVM classifier preoperatively.


Assuntos
Neoplasias dos Ductos Biliares/diagnóstico por imagem , Colangiocarcinoma/diagnóstico por imagem , Isocitrato Desidrogenase/genética , Tomografia Computadorizada por Raios X/métodos , Adulto , Idoso , Neoplasias dos Ductos Biliares/genética , Colangiocarcinoma/genética , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Estudos Retrospectivos , Máquina de Vetores de Suporte
15.
Chemosphere ; 272: 129635, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33486456

RESUMO

Phenanthrene (Phe) is a representative polycyclic aromatic hydrocarbon, and its ubiquity makes the risk assessment of Phe in aquatic ecosystems important. To assess the long-term effects of early-life Phe exposure on fish, the embryos of the model organism, zrbrafish (Danio rerio) were exposed to Phe at 0.05, 0.5, 5 and 50 nmol/L for 96 h and then raised to adulthood in clean water. Gonad development and reproductive functions were investigated in 120 day-old fish. The results showed that the percentage of spermatozoa in males and mature oocytes in females were decreased. The spawned egg numbers and the fertilization rate were reduced when the treated fish were mated with untreated fish. The transcription of genes involved in the brain-pituitary-gonadal axis was downregulated. The levels of both 17ß-estradiol and testosterone were significantly decreased in the 5 and 50 nmol/L groups compared with the control group. The methylation levels in the promotor of gnrh3 (encoding gonadotropin releasing hormone) were significantly elevated in the adult fish in the 5 and 50 nmol/L treatments, which might be associated with the downregulation of gnrh3 transcription. These results suggested that embryonic exposure to Phe can inhibit the reproductive ability of adult fish, which should be adequately emphasized in its risk assessment.


Assuntos
Fenantrenos , Poluentes Químicos da Água , Animais , Ecossistema , Feminino , Gônadas , Masculino , Fenantrenos/toxicidade , Reprodução , Poluentes Químicos da Água/toxicidade , Peixe-Zebra
16.
Toxins (Basel) ; 13(1)2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33450861

RESUMO

Dry-cured meat products are worldwide food with high-salt content, and filamentous fungi are beneficial to the maturation process. However, some salt-tolerant strains of Aspergillus and Penicillium produce ochratoxin A (OTA) on these products and thus threaten food safety. In our study, proteomic analysis was performed to reveal the mechanism of adaptability to high-salt environment by Aspergillus ochraceus. Twenty g/L and 70 g/L NaCl substrates were used to provide medium- and high-NaCl content environments, respectively. The NaCl addition could induce fungal growth, but only 20 g/L NaCl addition could induce spore production while 70 g/L repressed it. Proteomics analysis identified 2646 proteins in A. ochraceus fc-1, of which 237 and 251 were differentially expressed with 20 g/L and 70 g/L NaCl addition, respectively. Potential factors affecting fungal growth and development were identified by GO and KEGG analyses of biological process, cellular component, and molecular function terms. The results revealed that ergosterol synthesis pathway was significantly upregulated with 20 g/L and 70 g/L NaCl addition. However, fungal growth and development including OTA production were complex processes associated with many factors including nutrient uptake, cell membrane integrity, cell cycle, energy metabolism, intracellular redox homeostasis, protein synthesis and processing, autophagy, and secondary metabolism. Reactive oxygen species may be an important window to understand the mechanism that medium-salt content was conducive to intracellular signal transduction while high-salt content caused oxidative stress. The findings would help to improve the processes and storage conditions of dry-cured meat products.


Assuntos
Aspergillus ochraceus/fisiologia , Ocratoxinas/análise , Proteoma/análise , Cloreto de Sódio/química , Animais , Meios de Cultura/química , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Micotoxinas/análise
17.
Cell Metab ; 33(3): 581-597.e9, 2021 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-33440166

RESUMO

The health effect of dietary fat has been one of the most vexing issues in the field of nutrition. Few animal studies have examined the impact of high-fat diets on lifespan by controlling energy intake. In this study, we found that compared to a normal diet, an isocaloric moderately high-fat diet (IHF) significantly prolonged lifespan by decreasing the profiles of free fatty acids (FFAs) in serum and multiple tissues via downregulating FFA anabolism and upregulating catabolism pathways in rats and flies. Proteomics analysis in rats identified PPRC1 as a key protein that was significantly upregulated by nearly 2-fold by IHF, and among the FFAs, only palmitic acid (PA) was robustly and negatively associated with the expression of PPRC1. Using PPRC1 transgenic RNAi/overexpression flies and in vitro experiments, we demonstrated that IHF significantly reduced PA, which could upregulate PPRC1 through PPARG, resulting in improvements in oxidative stress and inflammation and prolonging the lifespan.

18.
Am J Prev Med ; 60(2): e85-e94, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33168338

RESUMO

CONTEXT: This systematic review aims to (1) characterize strategies used to identify individuals at increased risk for hereditary breast and ovarian cancer syndrome and Lynch syndrome outside of oncology and clinical genetic settings, (2) describe the extent to which these strategies have extended the reach of genetic services to underserved target populations, and (3) summarize indicators of the potential scalability of these strategies. EVIDENCE ACQUISITION: Investigators searched PubMed, EMBASE, and PsycINFO for manuscripts published from October 2005 to August 2019. Eligible manuscripts were those published in English, those that described strategies to identify those at risk for hereditary breast and ovarian cancer syndrome or Lynch syndrome, those implemented outside of an oncology or genetic specialty clinic, and those that included measures of cancer genetic services uptake. This study assessed strategies used to increase the reach of genetic risk screening and counseling services. Each study was evaluated using the 16-item quality assessment tool, and results were reported according to the PRISMA guidelines. EVIDENCE SYNTHESIS: Of the 16 eligible studies, 11 were conducted in clinical settings and 5 in public health settings. Regardless of setting, most (63%, 10/16) used brief screening tools to identify people with a family history suggestive of hereditary breast and ovarian cancer syndrome or Lynch syndrome. When reported, genetic risk screening reach (range =11%-100%) and genetic counseling reach (range =11%-100%) varied widely across studies. Strategies implemented in public health settings appeared to be more successful (median counseling reach=65%) than those implemented in clinical settings (median counseling reach=26%). Most studies did not describe fundamental components relevant for broad scalability. CONCLUSIONS: Efforts to expand cancer genomic services are limited outside of traditional oncology and genetic clinics. This is a missed opportunity because evidence thus far suggests that these efforts can be successful in expanding the reach of genetic services with the potential to reduce health inequities in access. This review highlights the need for accelerating research that applies evidence-based implementation strategies and frameworks along with process evaluation to understand barriers and facilitators to scalability of strategies with high reach.


Assuntos
Aconselhamento Genético , Neoplasias , Aconselhamento , Testes Genéticos , Genômica , Humanos , Neoplasias/diagnóstico , Neoplasias/genética
19.
Neuroinformatics ; 19(2): 267-284, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-32754778

RESUMO

The extreme complexity of mammalian brains requires a comprehensive deconstruction of neuroanatomical structures. Scientists normally use a brain stereotactic atlas to determine the locations of neurons and neuronal circuits. However, different brain images are normally not naturally aligned even when they are imaged with the same setup, let alone under the differing resolutions and dataset sizes used in mesoscopic imaging. As a result, it is difficult to achieve high-throughput automatic registration without manual intervention. Here, we propose a deep learning-based registration method called DeepMapi to predict a deformation field used to register mesoscopic optical images to an atlas. We use a self-feedback strategy to address the problem of imbalanced training sets (sampling at a fixed step size in nonuniform brains of structures and deformations) and use a dual-hierarchical network to capture the large and small deformations. By comparing DeepMapi with other registration methods, we demonstrate its superiority over a set of ground truth images, including both optical and MRI images. DeepMapi achieves fully automatic registration of mesoscopic micro-optical images, even macroscopic MRI datasets, in minutes, with an accuracy comparable to those of manual annotations by anatomists.


Assuntos
Mapeamento Encefálico/métodos , Encéfalo/diagnóstico por imagem , Processamento de Imagem Assistida por Computador/métodos , Imageamento por Ressonância Magnética/métodos , Rede Nervosa/diagnóstico por imagem , Redes Neurais de Computação , Animais , Mapeamento Encefálico/normas , Bases de Dados Factuais/normas , Humanos , Processamento de Imagem Assistida por Computador/normas , Imageamento Tridimensional/métodos , Imageamento Tridimensional/normas , Imageamento por Ressonância Magnética/normas , Camundongos , Camundongos Endogâmicos C57BL , Neuroimagem/métodos , Neuroimagem/normas , Projetos de Pesquisa/normas
20.
Magn Reson Med ; 85(3): 1455-1467, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-32989816

RESUMO

PURPOSE: To accelerate T2 mapping with highly sparse sampling by integrating deep learning image priors with low-rank and sparse modeling. METHODS: The proposed method achieves high-speed T2 mapping by highly sparsely sampling (k, TE)-space. Image reconstruction from the undersampled data was done by exploiting the low-rank structure and sparsity in the T2 -weighted image sequence and image priors learned from training data. The image priors for a single TE were generated from the public Human Connectome Project data using a tissue-based deep learning method; the image priors were then transferred to other TEs using a generalized series-based method. With these image priors, the proposed reconstruction method used a low-rank model and a sparse model to capture subject-dependent novel features. RESULTS: The proposed method was evaluated using experimental data obtained from both healthy subjects and tumor patients using a turbo spin-echo sequence. High-quality T2 maps at the resolution of 0.9 × 0.9 × 3.0 mm3 were obtained successfully from highly undersampled data with an acceleration factor of 8. Compared with the existing compressed sensing-based methods, the proposed method produced significantly reduced reconstruction errors. Compared with the deep learning-based methods, the proposed method recovered novel features better. CONCLUSION: This paper demonstrates the feasibility of learning T2 -weighted image priors for multiple TEs using tissue-based deep learning and generalized series-based learning. A new method was proposed to effectively integrate these image priors with low-rank and sparse modeling to reconstruct high-quality images from highly undersampled data. The proposed method will supplement other acquisition-based methods to achieve high-speed T2 mapping.


Assuntos
Aprendizado Profundo , Algoritmos , Encéfalo/diagnóstico por imagem , Voluntários Saudáveis , Humanos , Processamento de Imagem Assistida por Computador , Imageamento por Ressonância Magnética
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