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1.
Trials ; 20(1): 729, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842982

RESUMO

BACKGROUND: A considerable amount of randomized controlled trials (RCTs) have been published on statins and/or fibrates for diabetic retinopathy, a clinical condition associated with high social and economic burden. Adherence to the CONSORT statement items is imperative to ensure transparency and reproducibility in clinical research. The aim of this study is to assess the reporting quality and the adherence to CONSORT of RCTs assessing statins and/or fibrates for diabetic retinopathy. METHODS: We conducted a critical appraisal study at Discipline of Evidence-based Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo (Unifesp). A sensitive literature search was performed to identify all relevant RCTs, with no time or language limits. Two authors independently evaluated the reporting quality of the selected RCTs using the CONSORT statement as a standard. RESULTS: Thirteen reports of RCTs were included in this study. The adherence of the reports to CONSORT items ranged from 24% to 68%. The median score was 11 (interquartile range (IQR) 8 to 13). When analyzed separately, the methods sections of the reports had a median of three items (IQR 2 to 4) judged adherent to the methods items of CONSORT (items 3 to 12). The most underreported items were those related to trial design, title and abstract, allocation concealment, implementation of the randomization sequence, and blinding. Other important items, such as the one related to the description of the inclusion criteria, also had low adherence. CONCLUSIONS: The overall adherence to the CONSORT checklist items was poor, especially in the items related to the methods section. RCT reports on statins and/or fibrates for diabetic retinopathy must be optimized to avoid reporting biases and to improve transparency and reproducibility.

2.
Trials ; 20(1): 729-735, dez., 2019. tab., graf.
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: biblio-1049617

RESUMO

BACKGROUND: A considerable amount of randomized controlled trials (RCTs) have been published on statins and/or fibrates for diabetic retinopathy, a clinical condition associated with high social and economic burden. Adherence to the CONSORT statement items is imperative to ensure transparency and reproducibility in clinical research. The aim of this study is to assess the reporting quality and the adherence to CONSORT of RCTs assessing statins and/or fibrates for diabetic retinopathy. METHODS: We conducted a critical appraisal study at Discipline of Evidence-based Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo (Unifesp). A sensitive literature search was performed to identify all relevant RCTs, with no time or language limits. Two authors independently evaluated the reporting quality of the selected RCTs using the CONSORT statement as a standard. RESULTS: Thirteen reports of RCTs were included in this study. The adherence of the reports to CONSORT items ranged from 24% to 68%. The median score was 11 (interquartile range (IQR) 8 to 13). When analyzed separately, the methods sections of the reports had a median of three items (IQR 2 to 4) judged adherent to the methods items of CONSORT (items 3 to 12). The most underreported items were those related to trial design, title and abstract, allocation concealment, implementation of the randomization sequence, and blinding. Other important items, such as the one related to the description of the inclusion criteria, also had low adherence. CONCLUSIONS: The overall adherence to the CONSORT checklist items was poor, especially in the items related to the methods section. RCT reports on statins and/or fibrates for diabetic retinopathy must be optimized to avoid reporting biases and to improve transparency and reproducibility. (AU)


Assuntos
Ensaios Clínicos como Assunto , Viés de Publicação , Inibidores de Hidroximetilglutaril-CoA Redutases , Retinopatia Diabética , Hipolipemiantes
3.
Biochem Biophys Res Commun ; 519(3): 597-604, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31540690

RESUMO

INTRODUCTION: Multiple myeloma (MM) remains incurable due to high rates of relapse after various treatment regimens. WEE1 is a cell cycle related gene that regulates the G2/M checkpoint and promotes cell cycle suspension for consequent DNA repair. To date, there are clinical studies for the evaluation of WEE1 inhibitors in the treatment of solid tumors and studies on cell lines of non-MM hematological tumors. OBJECTIVES: To perform in vitro functional studies to verify the effect of the inhibition of WEE1 on MM cell lines viability and its potential as therapeutic target. MATERIAL AND METHODS: WEE1 expression was evaluated in 22 newly diagnosed MM patients and in four MM cell lines, RPMI-8226, U266 and SKO-007 and SK-MM2, by quantitative real-time PCR (qPCR). After treatment with the WEE1 inhibitor (MK-1775), with or without proteasome inhibitor (bortezomib) pretreatment, we assessed cell viability through Prestoblue functional test, microspheres formation in soft agar, and induction of apoptosis and cell cycle alterations by flow cytometry. RESULTS: All MM cell lines showed WEE1 expression by qPCR. RPMI-8226 and U266 showed a 50% reduction in cell viability after 24 h of incubation with MK-1775, at concentrations of 5 µM and 20 µM, respectively. SKO-007 showed dose and time dependence to this drug. Combination therapy with bortezomib and MK-1775 abolished the formation of soft agar microspheres in the RPMI-8226 cell line (also responsive to the use of both drugs) and U266, but SKO-007 was resistant to all drugs, isolated and combined. However, treatment of bortezomib followed by MK-1775 (sequential treatment) versus bortezomib alone showed statistically significant impact on cell lines total apoptosis: 88.8% vs 74.1% in RPMI-8222 (confirmed by cell cycle experiments); 92.5% vs 86.6% in U266; and 60.2% 30.9% on SKO-007 (p < 0.05). CONCLUSION: The sequential combination of bortezomib and WEE1 inhibitor, MK-1775, induced apoptosis in RPMI-8226, U266, and especially SKO-007 cell lines, more efficiently than the use of the same isolated drugs, highlighting its effect in inhibition of proliferation of tumor cells in MM cell lines. Our data suggest that WEE1 can figure as a MM target and that the sequential combination of bortezomib and MK-1775 may be explored in future clinical trials.

4.
An Bras Dermatol ; 91(5): 670-672, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27828649

RESUMO

Gastric syphilis is an uncommon extracutaneous manifestation of syphilis, occurring in less than 1% of patients, presenting nonspecific clinical manifestations. In general, it occurs on secondary stage. The critical point is the recognition of the syphilitic gastric involvement, without which there may be incorrect diagnosis of malignancy of the digestive tract. In this report, a case of secondary syphilis with gastric involvement that had complete remission with benzathine penicillin will be described.


Assuntos
Gastropatias/diagnóstico , Sífilis/diagnóstico , Antibacterianos/uso terapêutico , Biópsia , Endoscopia Gastrointestinal , Humanos , Masculino , Pessoa de Meia-Idade , Penicilina G Benzatina/uso terapêutico
5.
An. bras. dermatol ; 91(5): 670-672, Sept.-Oct. 2016. graf
Artigo em Inglês | LILACS | ID: biblio-827749

RESUMO

Abstract: Gastric syphilis is an uncommon extracutaneous manifestation of syphilis, occurring in less than 1% of patients, presenting nonspecific clinical manifestations. In general, it occurs on secondary stage. The critical point is the recognition of the syphilitic gastric involvement, without which there may be incorrect diagnosis of malignancy of the digestive tract. In this report, a case of secondary syphilis with gastric involvement that had complete remission with benzathine penicillin will be described.


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Gastropatias/diagnóstico , Sífilis/diagnóstico , Penicilina G Benzatina/uso terapêutico , Biópsia , Endoscopia Gastrointestinal , Antibacterianos/uso terapêutico
6.
An Bras Dermatol ; 91(2): 231-4, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27192527

RESUMO

Sarcoidosis is a disease which still has uncertain etiology. Possible environmental causes are cited in the literature, like organic and inorganic particles and infectious agents. Recent studies have demonstrated the occurrence of sarcoidosis in patients with chronic C hepatitis; however, this association remains without statistical or causal evidence. In this report a case of sarcoidosis associated with chronic hepatitis C will be described, with subcutaneous lesions, considered rare, and good response to treatment with colchicine and prednisone. The hepatitis C virus was isolated in sarcoid tissue and the association between the two diseases will be discussed.


Assuntos
Anti-Inflamatórios/uso terapêutico , Colchicina/uso terapêutico , Glucocorticoides/uso terapêutico , Hepatite C Crônica/tratamento farmacológico , Prednisona/uso terapêutico , Sarcoidose/tratamento farmacológico , Feminino , Hepatite C Crônica/complicações , Humanos , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Sarcoidose/etiologia , Sarcoidose/patologia , Resultado do Tratamento
7.
An. bras. dermatol ; 91(2): 231-234, Mar.-Apr. 2016. graf
Artigo em Inglês | LILACS | ID: lil-781365

RESUMO

Abstract Sarcoidosis is a disease which still has uncertain etiology. Possible environmental causes are cited in the literature, like organic and inorganic particles and infectious agents. Recent studies have demonstrated the occurrence of sarcoidosis in patients with chronic C hepatitis; however, this association remains without statistical or causal evidence. In this report a case of sarcoidosis associated with chronic hepatitis C will be described, with subcutaneous lesions, considered rare, and good response to treatment with colchicine and prednisone. The hepatitis C virus was isolated in sarcoid tissue and the association between the two diseases will be discussed.


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Sarcoidose/tratamento farmacológico , Prednisona/uso terapêutico , Colchicina/uso terapêutico , Hepatite C Crônica/tratamento farmacológico , Glucocorticoides/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Sarcoidose/etiologia , Sarcoidose/patologia , Reação em Cadeia da Polimerase , Resultado do Tratamento , Hepatite C Crônica/complicações
8.
An Bras Dermatol ; 90(3 Suppl 1): 235-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26312727

RESUMO

Eccrine syringofibroadenoma is a rare benign adnexal neoplasm derived from cells of the acrosyringium of eccrine sweat glands. ESFA usually manifests as a solitary nodule on the extremities of elderly patients, but it may also present as papules, nodules or plaques. Its clinical appearance is nonspecific and malignant neoplasms should be considered in the differential diagnosis. However, histopathological findings are typical. The main treatment is surgical excision. In order to illustrate a typical presentation of the tumor, we report a case of solitary eccrine syringofibroadenoma, including the surgical treatment used and its result.


Assuntos
Glândulas Écrinas/patologia , Fibroadenoma/patologia , Neoplasias das Glândulas Sudoríparas/patologia , Siringoma/patologia , Idoso de 80 Anos ou mais , Tornozelo , Biópsia , Glândulas Écrinas/cirurgia , Feminino , Fibroadenoma/cirurgia , Humanos , Neoplasias das Glândulas Sudoríparas/cirurgia , Siringoma/cirurgia , Resultado do Tratamento
9.
An. bras. dermatol ; 90(3,supl.1): 235-238, May-June 2015. ilus
Artigo em Inglês | LILACS | ID: lil-755753

RESUMO

Abstract

Eccrine syringofibroadenoma is a rare benign adnexal neoplasm derived from cells of the acrosyringium of eccrine sweat glands. ESFA usually manifests as a solitary nodule on the extremities of elderly patients, but it may also present as papules, nodules or plaques. Its clinical appearance is nonspecific and malignant neoplasms should beconsidered in the differential diagnosis. However, histopathological findings are typical. The main treatment is surgical excision. In order to illustrate a typical presentation of the tumor, we report a case of solitary eccrine syringofibroadenoma, including the surgical treatment used and its result.

.


Assuntos
Idoso de 80 Anos ou mais , Feminino , Humanos , Glândulas Écrinas/patologia , Fibroadenoma/patologia , Neoplasias das Glândulas Sudoríparas/patologia , Siringoma/patologia , Tornozelo , Biópsia , Glândulas Écrinas/cirurgia , Fibroadenoma/cirurgia , Neoplasias das Glândulas Sudoríparas/cirurgia , Siringoma/cirurgia , Resultado do Tratamento
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