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1.
Artigo em Inglês | MEDLINE | ID: mdl-33512817

RESUMO

Proteoglycans are involved in tumor development and may regulate the Hedgehog (HH) pathway. This study aimed to investigate the gene and protein expression of glypican-1 (GPC1), -3 (GPC3), and -5 (GPC5) in oral squamous cell carcinoma (OSCC) and tumor-free lateral margins (TM) and their association with the HH pathway. Quantitative PCR was performed for GPC1, GPC3, GPC5, SHH, PTCH1, SMO, and GLI1 genes in samples of OSCC (n=31), TM (n=12), and non-neoplastic oral mucosa (NNM) of healthy patients (n=6), alongside an immunohistochemical evaluation of GPC1, GPC3, and GPC5 proteins and HH proteins SHH and glioma-associated oncogene homolog 1 (GLI1). Double staining for GPC3/SHH, GPC5/SHH, GPC3/tubulin [ac Lys40], GPC5/Tubulin [ac Lys40], and GPC5/GLI1 was also performed. Overexpression of GPC1 and GPC5 in tumor samples and underexpressed levels of GPC3 gene transcripts were observed when compared with TM (standard sample). HH pathway mRNA aberrant expression in OSCC samples and a negative correlation between GPC1 and GPC5 at transcription levels were detected. GPC1 staining was rare in OSCC, but positive cells were found in NNM and TM. Otherwise positive immunostaining for GPC3 and GPC5 was observed in OSCCs, but not in NNM and TM. Blood vessels adjacent to tumor islands were positive for GPC1 and GPC5. Co-localization of GPC3-positive and GPC5-positive cells with SHH and Tubulin [ac Lys40] proteins was noted, as well as of GPC5 and GLI1. The absence of the GPC1 protein in neoplastic cells, underexpression of the GPC3 gene, and co-localization of GPCs and HH proteins may indicate the maintenance of aberrant HH pathway activation in OSCC.

2.
J Mol Histol ; 51(6): 675-684, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33000351

RESUMO

The purpose of this study was to evaluate the expression of Hedgehog (HH) signaling molecules (SHH and GLI-1) by cancer-associated fibroblasts (CAF) in oral squamous cell carcinoma (OSCC). Immunohistochemistry was used to detect molecular HH signaling and CAF-related protein expression, including α-SMA and S100A4, in 70 samples of human OSCC. The colocalization of α-SMA and S100A4 with SHH was also evaluated by double-staining. In vitro study was performed using primary normal oral fibroblast (NOF) and CAF through immunofluorescence and Western Blot for CAF-proteins, SHH, and GLI-1. Forty-five cases (64.28%) were positive for α-SMA exclusively in tumor stroma, and S100A4 was identified in the cytoplasm of CAFs in 94.28% (n = 66) of the cases. With respect to stromal cells, 64 (91.43%) OSCC cases were positive for SHH, and 31 were positive for GLI-1 (44.29%); positive correlations were found between SHH and α-SMA (p < 0.0001, φ = 0.51), as well as between SHH and S100A4 (p = 0.087, φ = 0.94). Protein expression of SHH and GLI-1 was observed in primary CAFs and NOFs. Although SHH was found to be localized in the cellular cytoplasm of both cell types, GLI-1 was present only in the nuclei of CAF. Our results indicate that CAFs are not only potential sources of HH ligands in tumor stroma, but may also respond to HH signaling through nuclear GLI-1 activation. We further observed that elevated SHH expression by OSCC cells was associated with higher CAF density, reinforcing the chemoattractant role played by these molecules.

3.
J Robot Surg ; 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33009987

RESUMO

To compare tissue trauma between Retropubic Radical Prostatectomy and Robotically Assisted Laparoscopic Radical Prostatectomy by inflammatory mediators. Serum samples from 40 patients submitted to RALP and 20 patients submitted to RRP were withdrawn at four different time points. The cytokines IL-4, IL-8, IL-6, IL-1B, IL-10 and TNF-α were detected using ELISA/Multiplex assays and xMAP-Luminex®. With both techniques, IL-10 and IL-6 were higher in T4 than in T1-T3 (p = 0.001). IL-10 and IL-6 were higher in T4 in open surgery than in robotic surgery (p = 0.000 and p = 0.001, respectively). Compared with both groups, IL-6 and IL-10 were higher in T4 in open surgery than in robotic surgery. Thus, we can postulate that RALP causes less tissue trauma than classical RRP, as indicated by the more limited increase in inflammatory mediators such as IL-6 and IL-10.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32769440

RESUMO

In oral squamous cell carcinoma (OSCC), involvement and activation of the Hedgehog pathway (HH) may be related to epithelial-mesenchymal transition and cell proliferation. The present study aimed to evaluate epithelial-mesenchymal transition and proliferative potential in OSCC cases demonstrating activation of the HH pathway. Twenty-three GLi-1-positive OSCC cases were submitted to immunohistochemical detection of Snail, Slug, N-cadherin, E-cadherin, ß-catenin, and MCM3 proteins. Clinical-pathologic immunoexpression data were obtained from the invasion front and tumor islets, and then compared. At the invasion front, OSCC cases presented positive Snail, Slug, and MCM3 expression in the nuclei of tumor cells. Loss of membrane and cytoplasmic expression of E-cadherin and ß-catenin was also observed. Positive N-cadherin expression was observed in 31.78% of the cases. GLi-1 immunoexpression was associated with loss of membrane E-cadherin (P<0.001), membrane ß-catenin (P<0.001), and cytoplasmic ß-catenin (P=0.02) expression. In the tumor islets, we observed nuclear expression of GLi-1, Snail, Slug, and MCM3. E-cadherin and ß-catenin showed positivity in tumor cell membranes. Statistically significant positive correlations between GLi-1 and Snail (P=0.05), E-cadherin (P=0.01), and cytoplasmic ß-catenin (P=0.04) were found. GLi-1 was associated with clinical staging, while membrane ß-catenin expression was related to the presence of metastasis in lymph nodes and to clinical staging. The HH pathway may be involved in regulating the expression of the mesenchymal phenotype. The loss of membrane E-cadherin and ß-catenin expression was observed at the tumor front region, whereas cell adhesion protein expression was detected in tumor islets regardless of MCM3.

6.
Bernoche, Claudia; Timerman, Sergio; Polastri, Thatiane Facholi; Giannetti, Natali Schiavo; Siqueira, Adailson Wagner da Silva; Piscopo, Agnaldo; Soeiro, Alexandre de Matos; Reis, Amélia Gorete Afonso da Costa; Tanaka, Ana Cristina Sayuri; Thomaz, Ana Maria; Quilici, Ana Paula; Catarino, Andrei Hilário; Ribeiro, Anna Christina de Lima; Barreto, Antonio Carlos Pereira; Azevedo, Antonio Fernando Barros de Filho; Pazin, Antonio Filho; Timerman, Ari; Scarpa, Bruna Romanelli; Timerman, Bruno; Tavares, Caio de Assis Moura; Martins, Cantidio Soares Lemos; Serrano, Carlos Vicente Junior; Malaque, Ceila Maria Sant'Ana; Pisani, Cristiano Faria; Batista, Daniel Valente; Leandro, Daniela Luana Fernandes; Szpilman, David; Gonçalves, Diego Manoel; Paiva, Edison Ferreira de; Osawa, Eduardo Atsushi; Lima, Eduardo Gomes; Adam, Eduardo Leal; Peixoto, Elaine; Evaristo, Eli Faria; Azeka, Estela; Silva, Fabio Bruno da; Wen, Fan Hui; Ferreira, Fatima Gil; Lima, Felipe Gallego; Fernandes, Felipe Lourenço; Ganem, Fernando; Galas, Filomena Regina Barbosa Gomes; Tarasoutchi, Flavio; Souza, Germano Emilio Conceição; Feitosa, Gilson Soares Filho; Foronda, Gustavo; Guimarães, Helio Penna; Abud, Isabela Cristina Kirnew; Leite, Ivanhoé Stuart Lima; Linhares, Jaime Paula Pessoa Filho; Moraes, Junior João Batista de Moura Xavier; Falcão, João Luiz Alencar de Araripe; Ramires, Jose Antônio Franchini; Cavalini, José Fernando; Saraiva, José Francisco Kerr; Abrão, Karen Cristine; Pinto, Lecio Figueira; Bianchi, Leonardo Luís Torres; Lopes, Leonardo Nícolau Geisler Daud; Piegas, Leopoldo Soares; Kopel, Liliane; Godoy, Lucas Colombo; Tobase, Lucia; Hajjar, Ludhmila Abrahão; Dallan, Luís Augusto Palma; Caneo, Luiz Fernando; Cardoso, Luiz Francisco; Canesin, Manoel Fernandes; Park, Marcelo; Rabelo, Marcia Maria Noya; Malachias, Marcus Vinícius Bolívar; Gonçalves, Maria Aparecida Batistão; Almeida, Maria Fernanda Branco de; Souza, Maria Francilene Silva; Favarato, Maria Helena Sampaio; Carrion, Maria Julia Machline; Gonzalez, Maria Margarita; Bortolotto, Maria Rita de Figueiredo Lemos; Macatrão-Costa, Milena Frota; Shimoda, Mônica Satsuki; Oliveira-Junior, Mucio Tavares de; Ikari, Nana Miura; Dutra, Oscar Pereira; Berwanger, Otávio; Pinheiro, Patricia Ana Paiva Corrêa; Reis, Patrícia Feitosa Frota dos; Cellia, Pedro Henrique Moraes; Santos Filho, Raul Dias dos; Gianotto-Oliveira, Renan; Kalil Filho, Roberto; Guinsburg, Ruth; Managini, Sandrigo; Lage, Silvia Helena Gelas; Yeu, So Pei; Franchi, Sonia Meiken; Shimoda-Sakano, Tania; Accorsi, Tarso Duenhas; Leal, Tatiana de Carvalho Andreucci; Guimarães, Vanessa; Sallai, Vanessa Santos; Ávila, Walkiria Samuel; Sako, Yara Kimiko.
Arq. bras. cardiol ; 113(3): 449-663, Sept. 2019. tab, graf
Artigo em Português | LILACS, Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: biblio-1038561
7.
Environ Sci Pollut Res Int ; 26(30): 31000-31013, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31452122

RESUMO

The performance of both adsorption and heterogeneous photo-Fenton processes, combined for the first time for the treatment of a real winery wastewater (WW), was evaluated under different operational conditions. A Portuguese natural Ca-smectite (Ca-Sm) was applied in both processes, however, with different purposes: (1) as an adsorbent, which reveals great capacity to retain organic acids, and (2) as catalyst support for the production of an iron-based catalyst (Fe-Sm). Both Ca-Sm and Fe-Sm materials were characterized by XRD, FTIR, low temperature N2 adsorption isotherms, and SEM-EDX. The adsorption process was investigated under different experimental conditions, namely, different pH, adsorbent dosages, and different concentrations of the organic contaminant. The adsorption isotherm was successfully described by Jovanovich isothermal model (R2 = 0.990), which predicted a maximum adsorption capacity of 161 mg C/g. Regarding to the heterogeneous photo-Fenton process, the higher TOC removal percentage obtained (78.7% - 240 min) was achieved at pH 4.0, with a H2O2 concentration of 98 mM and a catalyst dosage (S:L, solid:liquid ratio) corresponding to 6.00 g/L (UV-C). As a result, the combination of both treatment processes, using the optimized conditions, allowed a total TOC removal of 90%, where the initial TOC0 (825 mg C/L) was reduced by 54%, through the adsorption process, and by 36% by means of heterogeneous photo-Fenton process [TOC0 = 825 mg C/L (1) - TOCf-ads = 380 mg C/L (2) - TOCf-pF = 81 mg C/L (3)].


Assuntos
Indústria de Processamento de Alimentos , Eliminação de Resíduos Líquidos/métodos , Adsorção , Catálise , Peróxido de Hidrogênio/química , Concentração de Íons de Hidrogênio , Resíduos Industriais , Ferro/química , Oxirredução , Silicatos , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Águas Residuárias/química , Vinho , Difração de Raios X
8.
Rev Soc Bras Med Trop ; 52: e20180522, 2019 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-31340358

RESUMO

Listeria is an unusual pathogen that causes neonatal infection with high morbidity and mortality. We present the case of a premature newborn whose mother had a rash during pregnancy; the newborn had severe early sepsis because of Listeria monocytogenes and histopathologically suggestive findings of the placenta. Obstetricians and neonatologists should suspect listeriosis in cases with compatible epidemiological history, clinical features, and examination findings of the placenta.


Assuntos
Doenças do Recém-Nascido/diagnóstico , Listeria monocytogenes/isolamento & purificação , Listeriose/microbiologia , Sepse/microbiologia , Adulto , Feminino , Humanos , Recém-Nascido , Doenças do Recém-Nascido/microbiologia , Transmissão Vertical de Doença Infecciosa , Unidades de Terapia Intensiva Neonatal , Listeriose/diagnóstico , Listeriose/transmissão , Masculino , Gravidez , Complicações Infecciosas na Gravidez , Sepse/diagnóstico , Adulto Jovem
9.
J. Health Biol. Sci. (Online) ; 7(3): 320-323, jul.-set. 2019.
Artigo em Português | LILACS | ID: biblio-1005681

RESUMO

Relato de caso: Relatar um caso de canal acessório do Canalis Sinuosos, localizado por palatino à raiz do dente 21 que, na radiografia panorâmica, foi observada como uma área radiolúcida sobreposta aos terços médio-apical dente 21, indicando reabsorção radicular, sendo indispensável uma investigação acurada por tomografia computadorizada de feixe cônico (TCFC). Conclusão: A TCFC se mostrou eficiente na identificação do Canalis Sinuosos em relação à radiografia panorâmica, possibilitando um correto diagnóstico para o caso.


Case report: This report describes a case of Canalis sinuosus accessory canal, lingually to the root of the tooth 21, that was observed on panoramic radiograph as a radiolucent area superimposed over the middle and apical thirds, indicating root resorption. However, cone-beam computed tomography (CBCT) recommended for an accurated diagnosis of the case disclose the presence of this anatomic variation. Conclusion: CBCT was more accurate in detection of Canalis sinuosus than panoramic radiography.


Assuntos
Nervo Maxilar , Tomografia Computadorizada de Feixe Cônico , Variação Anatômica
10.
Saúde Soc ; 28(2): 310-325, abr.-jun. 2019. tab, graf
Artigo em Português | LILACS | ID: biblio-1014576

RESUMO

Resumo Apesar dos esforços de despoluição desde os anos 1990, não houve percepção, por parte da opinião pública, de que houve evolução do deteriorado quadro social da região da bacia da Baía de Guanabara (RJ). A importância dos indicadores sociais emerge em um momento em que se faz necessário prestar contas à população quanto aos investimentos e resultados obtidos, direcionar as ações às questões sociais e locais em situação de maior urgência e acompanhar os resultados para a identificação de ajustes nas ações para o alcance de melhores resultados. A atual agenda de discussão das questões sociais do entorno da baía configurou a base para a elaboração de um sistema de indicadores sociais. Três bacias hidrográficas em processo de despoluição também foram estudadas, com foco em suas abordagens quanto às questões sociais. Um modelo teórico de indicadores foi elaborado e testado em um recorte da bacia da Baía de Guanabara, por intermédio da utilização de dados públicos disponíveis. O modelo se mostrou ferramenta útil para uma abordagem holística da bacia ao informar sobre o melhor direcionamento de ações de despoluição para resultados mais efetivos em ambas as questões, sociais e ambientais.


Abstract Despite the efforts in the depollution since the 1990s, evolution of the damaged social scenario in the region of the watershed of the Guanabara Bay is not perceived by the public opinion. The importance of social indicators emerges in a moment in which rendering account to the population regarding investiments and results obtained, orienting actions for emergency social and local issues and monitoring results for identification of adjustments to the actions for the achievement of better results is necessary. The current agenda for debating social issues of the region of the bay represented the basis for the creation of a system of social indicators. Three watersheds in depollution process were also studied, focusing on their approaches regarding social issues. A theoretical model of indicators was developed and tested in a draft of the Guanabara Bay watershed, using the public data available. The model proved to be a useful tool for an holistic approach of the bay by providing information on the better orientation of depollution actions for more effective results in both social and environmental issues.


Assuntos
Bacias Hidrográficas , Saúde Ambiental , Purificação da Água , Indicadores Sociais
11.
Rev. Soc. Bras. Med. Trop ; 52: e20180522, 2019. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1013320

RESUMO

Abstract Listeria is an unusual pathogen that causes neonatal infection with high morbidity and mortality. We present the case of a premature newborn whose mother had a rash during pregnancy; the newborn had severe early sepsis because of Listeria monocytogenes and histopathologically suggestive findings of the placenta. Obstetricians and neonatologists should suspect listeriosis in cases with compatible epidemiological history, clinical features, and examination findings of the placenta.

12.
Tumour Biol ; 40(11): 1010428318803011, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30400755

RESUMO

The acquisition of a castration-resistant prostate cancer phenotype by prostate cancer cells is the alteration that has the worst prognosis for patients. The aim of this study was to evaluate the role of the microRNAs-23b/-27b as well as the possible CCNG1 target gene in tissue samples from patients with localized prostate cancer that progressed to castration-resistant prostate cancer and in a castration-resistant prostate cancer cell line (PC-3). The microRNAs and target gene expression levels of the surgical specimens were analyzed by quantitative real-time polymerase chain reaction. The prostate cancer cell line, PC-3, was transfected with pre-miR-23b, pre-miR-27b, and their respective controls using Lipofectamine RNAiMAX and exposed or not to flutamide. After transfections, expression levels of both the microRNAs and the gene, CCNG1, were analyzed by quantitative real-time polymerase chain reaction. The apoptosis and cell cycle assays were performed on the mini MUSE cytometer. MicroRNAs-23b/-27b were underexpressed in surgical specimens of prostate cancer; however, their target gene, CCNG1, was overexpressed in 69% of the cases. After transfection with the microRNAs-23b/-27b and flutamide, we observed a reduction in gene expression compared with cells that were treated only with microRNAs or only with flutamide. In the apoptosis assay, we demonstrated cell sensitization following transfection with microRNAs-23b/-27b and potentiation when co-administered with flutamide. The number of cells in apoptosis was almost three times higher with the simultaneous treatments (miR + flutamide) compared with the control (p < 0.05). In the cell cycle assay, only flutamide treatment showed better results; a higher number of cells were found in the G0-G1 phase, and a lower percentage of cells completed the final phase of the cycle (p < 0.05). We conclude that microRNAs-23b/-27b are downexpressed in prostate cancer, and their target gene, CCNG1, is overexpressed. We postulated that microRNAs-23b/-27b sensitize the PC-3 cell line and that after the addition of flutamide in the apoptosis assay, we would observe synergism in the treatments between miR and flutamide. In the cell cycle assay, the use of flutamide was sufficient to decrease the number of cells in mitosis. Therefore, we postulate that microRNAs, along with other drugs, may become very useful therapeutic tools in the treatment of castration-resistant prostate cancer.


Assuntos
Apoptose/efeitos dos fármacos , Apoptose/genética , Ciclina G1/genética , Flutamida/metabolismo , MicroRNAs/genética , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , Neoplasias de Próstata Resistentes à Castração/genética , Linhagem Celular Tumoral , Fase G1/efeitos dos fármacos , Fase G1/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Pessoa de Meia-Idade , Mitose/efeitos dos fármacos , Mitose/genética , Próstata/efeitos dos fármacos , Fase de Repouso do Ciclo Celular/efeitos dos fármacos , Fase de Repouso do Ciclo Celular/genética , Transfecção/métodos
13.
Cien Saude Colet ; 23(10): 3413-3420, 2018 Oct.
Artigo em Português | MEDLINE | ID: mdl-30365860

RESUMO

The scope of this article is to estimate the prevalence and factors associated with alcohol use during pregnancy. It involved a cross-sectional study in a sample of 361 pregnant women in a reference service for gynecological and prenatal care. The data related to socio-demographic characteristics, alcohol use and potential associated factors were collected through face-to-face interviews. Poisson regression with robust variance was used to identify factors associated with the outcome analyzed. The consumption of alcohol in the sample was 17.7% (95% CI: 95% CI: 14.1% to 22.0%). A history of pre-gestational or gestational diabetes, suicidal ideation and tobacco use in the last 30 days was associated with alcohol use during pregnancy (p < 0.05). The study showed a high prevalence of alcohol use during the current pregnancy and its association with important factors. Actions such as screening for alcohol and advice on problems associated with the use of this substance, especially during the prenatal period, can contribute to effective reduction of alcohol use in pregnant women and related maternal and fetal injuries.


Assuntos
Consumo de Bebidas Alcoólicas/epidemiologia , Programas de Rastreamento/métodos , Complicações na Gravidez/epidemiologia , Adolescente , Adulto , Consumo de Bebidas Alcoólicas/prevenção & controle , Brasil/epidemiologia , Estudos Transversais , Diabetes Gestacional/epidemiologia , Feminino , Maternidades , Humanos , Entrevistas como Assunto , Gravidez , Cuidado Pré-Natal/métodos , Prevalência , Fatores de Risco , Ideação Suicida , Uso de Tabaco/epidemiologia , Adulto Jovem
14.
Oncotarget ; 9(69): 33077-33097, 2018 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-30237853

RESUMO

Novel biomarkers are needed to complement prostate specific antigen (PSA) in prostate cancer (PCa) diagnostic screening programs. Glycoproteins represent a hitherto largely untapped resource with a great potential as specific and sensitive tumor biomarkers due to their abundance in bodily fluids and their dynamic and cancer-associated glycosylation. However, quantitative glycoproteomics strategies to detect potential glycoprotein cancer markers from complex biospecimen are only just emerging. Here, we describe a glycoproteomics strategy for deep quantitative mapping of N- and O-glycoproteins in urine with a view to investigate the diagnostic value of the glycoproteome to discriminate PCa from benign prostatic hyperplasia (BPH), two conditions that remain difficult to clinically stratify. Total protein extracts were obtained, concentrated and digested from urine of six PCa patients (Gleason score 7) and six BPH patients. The resulting peptide mixtures were TMT-labeled and mixed prior to a multi-faceted sample processing including hydrophilic interaction liquid chromatography (HILIC) and titanium dioxide SPE based enrichment, endo-/exoglycosidase treatment and HILIC-HPLC pre-fractionation. The isolated N- and O-glycopeptides were detected and quantified using high resolution mass spectrometry. We accurately quantified 729 N-glycoproteins spanning 1,310 unique N-glycosylation sites and observed 954 and 965 unique intact N- and O-glycopeptides, respectively, across the two disease conditions. Importantly, a panel of 56 intact N-glycopeptides perfectly discriminated PCa and BPH (ROC: AUC = 1). This study has generated a panel of intact glycopeptides that has a potential for PCa detection.

15.
Rev Soc Bras Med Trop ; 51(4): 508-512, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30133635

RESUMO

INTRODUCTION: This study reports the genotype prevalence of hepatitis C virus (HCV) in Pará, Brazil. METHODS: A retrospective cross-sectional study was conducted on 344 plasma samples sent to the Lacen-Pará for diagnostics by molecular techniques. RESULTS: HCV genotypes identified in the different regions of Pará were 1b (47.7%), 3 (23.3%), 1a (18%), and 2 (4.4%). Genotype 1 occurred in 41.6% of men and 30.8% of women in the 18-86-year-old group. CONCLUSIONS: Genotype 1 is the most predominant in Pará, which reinforces the idea of its relationship with late-diagnosed chronic infection.


Assuntos
Hepacivirus/genética , Hepatite C Crônica/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Brasil/epidemiologia , Estudos Transversais , Feminino , Genótipo , Hepatite C Crônica/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , RNA Viral/genética , Estudos Retrospectivos , Adulto Jovem
16.
Rev. Soc. Bras. Med. Trop ; 51(4): 508-512, July-Aug. 2018. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1041479

RESUMO

Abstract INTRODUCTION This study reports the genotype prevalence of hepatitis C virus (HCV) in Pará, Brazil. METHODS: A retrospective cross-sectional study was conducted on 344 plasma samples sent to the Lacen-Pará for diagnostics by molecular techniques. RESULTS: HCV genotypes identified in the different regions of Pará were 1b (47.7%), 3 (23.3%), 1a (18%), and 2 (4.4%). Genotype 1 occurred in 41.6% of men and 30.8% of women in the 18-86-year-old group. CONCLUSIONS: Genotype 1 is the most predominant in Pará, which reinforces the idea of its relationship with late-diagnosed chronic infection.

17.
Int J STD AIDS ; 29(7): 658-664, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29393006

RESUMO

The objective of this study was to investigate risk behaviors for sexually transmitted infections (STIs) in noninjecting drug users (NIDUs), using STI diagnosis history as an indicator. A cross-sectional study was conducted in 323 NIDUs of two facilities for alcohol and/or drug dependence treatment in the Goiás State, Central Brazil. All participants were interviewed about risk behaviors and STI history. Multivariable analysis was performed in order to identify predictors of STIs. Adjusted prevalence ratio (APR) with confidence intervals of 95% was obtained using a Poisson regression model. Prevalence of self-reported STIs in the previous 12 months was 25.4% (95% confidence interval [95% CI]: 21.0-30.4%). A multivariable model verified that age (APR: 1.01; 95% CI: 1.00-1.01), sexual contact with partners diagnosed with STIs (APR: 1.27; 95% CI: 1.12-1.45) and injecting drug users (IDUs) (APR: 1.14; 95% CI: 1.14; 95% CI: 1.01-1.31), exchange sex for money and/or drugs (APR: 1.12; 95% CI: 1.02-1.21), and a history of sexual violence (APR: 1.04; 95% CI: 1.04-1.32) were predictors of STIs. Elevated rates of STI history and risk behaviors were observed in NIDUs, supporting the vulnerability of this group for these infections. Public policies and health outreach should be intensified in this population, principally regular STI testing of individuals in treatment for drug dependence and their sexual partners.


Assuntos
Usuários de Drogas/psicologia , Assunção de Riscos , Comportamento Sexual , Doenças Sexualmente Transmissíveis/epidemiologia , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Adolescente , Adulto , Brasil/epidemiologia , Usuários de Drogas/estatística & dados numéricos , Feminino , Humanos , Drogas Ilícitas , Masculino , Fatores de Risco , Doenças Sexualmente Transmissíveis/diagnóstico , Transtornos Relacionados ao Uso de Substâncias/psicologia
18.
Ciênc. Saúde Colet ; 23(10): 3413-3420, Out. 2018. tab
Artigo em Português | LILACS | ID: biblio-974676

RESUMO

Resumo O objetivo deste artigo é estimar a prevalência e os fatores associados ao uso de álcool durante a gestação. Estudo de corte transversal em uma amostra de 361 gestantes de um serviço de referência à assistência ginecológica e pré-natal. Os dados relacionados às características sociodemográficas, uso de álcool e potenciais fatores associados foram coletados por meio de entrevista face a face. Análise de regressão de Poisson com variância robusta foi utilizada para verificar os fatores associados ao desfecho analisado. O consumo de álcool na amostra estudada foi de 17,7% (IC 95%: IC 95%: 14,1-22,0%). Antecedentes de diabetes pré-gestacional ou gestacional, ideação suicida e uso de tabaco nos últimos 30 dias foi associado ao uso de álcool durante a gestação (p < 0,05). O estudo apontou alta prevalência de uso de álcool na gestação atual e a sua associação com importantes fatores. Ações como rastreio para o álcool e aconselhamentos sobre os problemas associados ao uso dessa substância principalmente no pré-natal podem contribuir para redução efetiva ou anulação do seu uso em gestantes e agravos materno-fetais relacionados.


Abstract The scope of this article is to estimate the prevalence and factors associated with alcohol use during pregnancy. It involved a cross-sectional study in a sample of 361 pregnant women in a reference service for gynecological and prenatal care. The data related to socio-demographic characteristics, alcohol use and potential associated factors were collected through face-to-face interviews. Poisson regression with robust variance was used to identify factors associated with the outcome analyzed. The consumption of alcohol in the sample was 17.7% (95% CI: 95% CI: 14.1% to 22.0%). A history of pre-gestational or gestational diabetes, suicidal ideation and tobacco use in the last 30 days was associated with alcohol use during pregnancy (p < 0.05). The study showed a high prevalence of alcohol use during the current pregnancy and its association with important factors. Actions such as screening for alcohol and advice on problems associated with the use of this substance, especially during the prenatal period, can contribute to effective reduction of alcohol use in pregnant women and related maternal and fetal injuries.


Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto , Adulto Jovem , Complicações na Gravidez/epidemiologia , Consumo de Bebidas Alcoólicas/epidemiologia , Programas de Rastreamento/métodos , Cuidado Pré-Natal/métodos , Brasil/epidemiologia , Consumo de Bebidas Alcoólicas/prevenção & controle , Prevalência , Estudos Transversais , Entrevistas como Assunto , Fatores de Risco , Diabetes Gestacional/epidemiologia , Ideação Suicida , Uso de Tabaco/epidemiologia , Maternidades
19.
BMC Urol ; 17(1): 113, 2017 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-29202733

RESUMO

BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is the third most common urological cancer in adults. Our aim is to evaluate genes and miRNAs expression profiles involved with angiogenesis and tumor characteristics in ccRCC. METHODS: The expression levels of miRNAs miR-99a, 99b, 100; 199a; 106a; 106b; 29a; 29b; 29c; 126; 200a, 200b and their respective target genes: mTOR, HIF1-α, VHL, PDGF, VEGF, VEGFR1 and VEGFR2 were analyzed using qRT-PCR in tumor tissue samples from 56 patients with ccRCC. Five samples of benign renal tissue were utilized as control. The expression levels of miRNAs and genes were related to tumor size, Fuhrman nuclear grade and microvascular invasion. RESULTS: miR99a was overexpressed in most samples and its target gene mTOR was underexpressed, this also occurs for miRNAs 106a, 106b, and their target gene VHL. An increase in miR-200b was correlated with high-risk tumors (p = 0.01) while miR-126 overexpression was associated with Fuhrman's low grade (p = 0.03). CONCLUSIONS: Our results show that in ccRCC there are changes in miRNAs expression affecting gene expression that could be important in determining the aggressiveness of this lethal neoplasia.


Assuntos
Carcinoma de Células Renais/genética , Regulação Neoplásica da Expressão Gênica/genética , Neoplasias Renais/genética , MicroRNAs/genética , Neovascularização Patológica/genética , Carcinoma de Células Renais/metabolismo , Feminino , Humanos , Neoplasias Renais/metabolismo , Masculino , MicroRNAs/biossíntese , Pessoa de Meia-Idade , Neovascularização Patológica/metabolismo
20.
Materials (Basel) ; 10(10)2017 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-28934136

RESUMO

A synthesized functionalized pillared porous phosphate heterostructure (PPH), surface functionalized phenyl group, has been used to remove the dye Acid Blue 113 from wastewater. X-ray photoemission spectroscopy XPS and X-ray diffraction (XRD) were used to study its structure. The specific surface area of this was 498 m²/g. The adsorption capacities of PPH and phenyl surface functionalized (Φ-PPH) were 0.0400 and 0.0967 mmol/g, respectively, with a dye concentration of 10-5 M when well fitted with SIPS and Langmuir isotherms respectively (pH 6.5, 25 °C). The incorporation of the dye to the adsorbent material was monitored by the S content of the dye. It is suggested as an alternative for Acid Blue 113 remediation.

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