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1.
Bioengineered ; 12(1): 7920-7928, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34622713

RESUMO

We aimed to assess the effects of dexmedetomidine (DEX) on postoperative cognitive function of sleep deprivation (SD) rats based on changes in inflammatory response. Male rats were randomly divided into blank control (C), SD, DEX, and SD+DEX groups. The SD model was established through intraperitoneal injection of DEX. The escape latency was detected through Morris water maze test daily, and the mechanical withdrawal threshold and thermal withdrawal latency were detected for 8 d. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in hippocampus homogenate were determined, and the morphological changes in neurons were detected through Nissl staining. The concentration of interleukin-1ß (IL-1ß), tumor necrosis factor-α (TNF-α), and IL-6 in the hippocampus was detected by enzyme-linked immunosorbent assay, and the Rac1/protein kinase B (AKT)/nuclear factor-κB (NF-κB) expressions were detected by Western blotting. The changes in immunofluorescence localization of NF-κB were observed by confocal microscopy. Compared with SD group, the escape latency was shortened, original platform-crossing times increased, MDA content declined, SOD activity rose, neurons were arranged orderly and number of Nissl bodies increased in the hippocampal CA1 region, levels of IL-1ß, TNF-α, and IL-6 in the hippocampus decreased, Rac1/AKT/NF-κB expressions were down-regulated, and proportion of NF-κB entering the nucleus declined in SD+DEX group (P < 0.05). DEX can effectively alleviate postoperative hippocampal inflammation and improve cognitive function of SD rats. The ability of DEX to relieve oxidative stress of hippocampal neurons, restore damaged cells, and reduce hippocampal inflammation in SD rats may be related to the Rac1/AKT/NF-κB pathway.

2.
Sci Rep ; 11(1): 19909, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34620914

RESUMO

Heavy metals contaminations in mining areas aroused wide concerns globally. Efficient evaluation of its pollution status is a basis for further soil reclamation. Visible and near-infrared reflectance (Vis-NIR) spectroscopy has been diffusely used for retrieving heavy metals concentrations. However, the reliability and feasibility of calibrated models were still doubtful. The present study estimated zinc (Zn) concentrations via the random forest (RF) and partial least squares regression (PLSR) using ground in-situ Zn concentrations as well as soil spectral reflectance at an Opencast Coal Mine of Ordos, China in February 2020. The coefficient of determination (R2), root mean square error (RMSE), mean absolute error (MAE), and the ratio of performance to deviation (RPD) were selected to assess the robustness of the methods in estimating Zn contents. Moreover, the characteristic bands were chosen by Pearson correlation analysis and Boruta Algorithm. Finally, the comparison between RF and PLSR combined with eight spectral reflectance transformation methods was conducted for four concentration groups to determine the optimal model. The results indicated that: (1) Zn contents represented a skewed distribution (coefficient of variation (CV) = 33%); (2) the spectral reflectance tended to decrease with the increase of Zn contents during 580-1850 nm based on Savitzky-Golay smoothing (SG); (3) the continuous wavelet transform (CWT) demonstrated higher effectiveness than other spectral reflectance transformation methods in enhancing spectral responses, the R2 between Zn contents and the soil spectral reflectance achieved the highest (R2 = 0.71) by using CWT; (4) the RF combined with CWT exhibited the best performance than other methods in the current study (R2 = 0.97, RPD = 3.39, RMSE = 1.05 mg kg-1, MAE = 0.79 mg kg-1). The current study supplied a scientific scheme and theoretical support for predicting heavy metals concentrations via the Vis-NIR spectral method in possible contaminated areas such as coal mines and metallic mineral deposit areas.

3.
Mol Ecol Resour ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34549890

RESUMO

Populus has a wide ecogeographical range spanning the Northern Hemisphere, and interspecific hybrids are common. Populus tomentosa Carr. is widely distributed and cultivated in the eastern region of Asia, where it plays multiple important roles in forestry, agriculture, conservation, and urban horticulture. Reference genomes are available for several Populus species, however, our goals were to produce a very high quality de novo chromosome-level genome assembly in P. tomentosa genome that could serve as a reference for evolutionary and ecological studies of hybrid speciation throughout the genus. Here, combining long-read sequencing and Hi-C scaffolding, we present a high-quality, haplotype-resolved genome assembly. The genome size was 740.2 Mb, with a contig N50 size of 5.47 Mb and a scaffold N50 size of 46.68 Mb, consisting of 38 chromosomes, as expected with the known diploid chromosome number (2n = 2x = 38). A total of 59,124 protein-coding genes were identified. Phylogenomic analyses revealed that P. tomentosa is comprised of two distinct subgenomes, which we deomonstrate is likely to have resulted from hybridization between Populus adenopoda as the female parent and Populus alba var. pyramidalis as the male parent, with an origin of approximately 3.93 Ma. Although highly colinear, significant structural variation was found between the two subgenomes. Our study provides a valuable resource for ecological genetics and forest biotechnology.

4.
J Phys Condens Matter ; 33(49)2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34517354

RESUMO

We study the global quantum discord (GQD) in the Lipkin-Meshkov-Glick (LMG) model at zero and finite temperatures, in which all spins are mutually interacted and introduced in an external magnetic field (denoted byh). We confirm that the high coordinate number is one of the most distinguishing features of the LMG model, which directly results in the nontrivial behaviors of quantum correlations. We compare the GQD with other quantum correlations measures (such as concurrence, quantum discord, and global entanglement) and find the remarkable difference between them. For instance, we find that GQD spreads in the entire system and captures more information on quantum correlations when comparing with concurrence and quantum (pairwise) discord. We discover that GQD can characterize multipartite correlations in the both broken phase (h< 1) and the symmetric phase (h⩾ 1), while global entanglement and its generalized fail. Moreover, we show that the ground-state GQD can identify second-order quantum phase transitions of the LMG model in the thermodynamic limit. By making the scaling behavior of the GQD in the LMG model analysis, we show that GQD (denoted byG) scales asG∼k⋅N+cwithk> 0 in the anisotropic cases for any fixed magnetic field. We further show that GQD behaves asG|sn∼k⋅1N+cwithk< 0 in the isotropic cases for any Dicke state |sn⟩. Hereinkandcare the fitting parameters. We also find that the thermal stability of the GQD at low temperatures depends on the energy gap. We further reveal that the extraordinary behaviors of the thermal-state GQD in the isotropic LMG model are explained by the contribution theory of the energy levels.

5.
Exp Mol Med ; 53(9): 1307-1318, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34497345

RESUMO

TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little is known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized in the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 and Cdk4 and induced the expression or activity of stromal differentiation markers Prl8a2, Prl3c1 and ALP, indicating the importance of TAZ in decidualization. Knockdown of TAZ impeded HB-EGF induction of stromal cell proliferation and differentiation. Under oxidative stress, TAZ protected stromal differentiation against oxidative damage by reducing intracellular ROS and enhancing cellular antioxidant capacity dependent on the Nrf2/ARE/Foxo1 pathway. TAZ strengthened the transcriptional activity of Nrf2 which directly bound to the antioxidant response element (ARE) of Foxo1 promoter region. Additionally, silencing TAZ caused accumulation of intracellular ROS through heightening NOX activity whose blockade by APO reversed the disruption in stromal differentiation. Further analysis revealed that TAZ might restore mitochondrial function, as indicated by the increase in ATP level, mtDNA copy number and mitochondrial membrane potential with the reduction in mitochondrial superoxide. Additionally, TAZ modulated the activities of mitochondrial respiratory chain complexes I and III whose suppression by ROT and AA resulted in the inability of TAZ to defend against oxidative damage to stromal differentiation. Moreover, TAZ prevented stromal cell apoptosis by upregulating Bcl2 expression and inhibiting Casp3 activity and Bax expression. In summary, TAZ might mediate HB-EGF function in uterine decidualization through Ccnd3 and ameliorate oxidative damage to stromal cell differentiation via Nrf2/ARE/Foxo1 pathway.

6.
Int J Biol Macromol ; 191: 359-376, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34534587

RESUMO

MYB proteins are one of the most abundant transcription factor families in the plant kingdom. Evidence has increasingly revealed that MYB-related proteins function in diverse plant biological processes. However, little is known about the genome-wide characterization and functions of MYB-related proteins in Populus, an important model and commercial tree species. In this study, 152 PtrMYBRs were identified and unevenly located on 19 Populus chromosomes. A phylogenetic analysis divided them into six major subgroups, supported by conserved gene organization, consensus motifs, and protein domain architecture. Promoter assessment and gene ontology classification results indicated that the MYB-related family is likely involved in plant development and responses to various environmental stressors. The Populus MYB-related family members showed various expression patterns in different tissues and stress conditions, implying their crucial roles in the development and stress responses in Populus. Co-expression analyses revealed that Populus MYB-related genes might participate in the regulation of antioxidant defense system and various signaling pathways in response to stress. The three-dimensional structures of different subgroup of Populus MYB-related proteins further provided functional information at the proteomic level. These findings provide valuable information for a prospective functional dissection of MYB-related proteins and genetic improvement of Populus.

7.
Bioorg Chem ; 116: 105340, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34530236

RESUMO

Cancers are highly heterogeneous and typically contain a small subset of drug-resisting cells called tumor initiating cells or cancer stem cells (CSCs). CSCs can self-renew, divide asymmetrically, and often cause tumor invasion and metastasis. Therefore, treatments specifically targeting CSCs are critical to improve patient survival. Recently, we identified a highly specific peptidomimetic (peptoid - PCS2) that selectively binds to the CSC subpopulation of lung cancer over the remaining cancer cells (non-CSCs). Subsequently, we identified plectin as the target of PCS2. Plectin is an intracellular structural protein, which is involved in tumor invasion and metastasis when it appears on cell surface. While PCS2 monomer did not display any anti-cancer activity, we designed a series of homo-dimeric versions of PCS2, and identified PCS2D1.2 optimized homo-dimer that displayed highly specific cytotoxicity towards CSCs over non-CSCs. PCS2D1.2 effectively blocked the in vitro colony formation and cell migration, hallmarks of CSCs. Furthermore, PCS2D1.2 reduced the in vivo tumor formation. In both in vitro and in vivo studies, PCS2D1.2 effectively reduced plectin expression and/or plectin-rich CSCs, but had no effect on non-CSCs. Therefore, PCS2D1.2 has the potential to be developed as a highly CSC specific drug candidate, which can be used in combination with current anti-cancer drugs.

8.
Food Funct ; 12(18): 8800-8811, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34374402

RESUMO

Polycystic ovarian syndrome (PCOS) is a complex endocrinopathy in women of reproductive age and the main cause of female infertility, but there is no universal drug for PCOS therapy. As a predominant dietary isoflavone present in soybeans, genistein (GEN) possesses estrogenic and antioxidative properties, but limited information is available regarding its therapeutic potential and underlying molecular mechanism in PCOS. In this study, we found that GEN might restore the estrous cycle of PCOS mice and ameliorate the elevation of circulating T, AMH and LH levels as well as LH/FSH ratios along with reduced cystic follicles, indicating the importance of GEN in PCOS therapy. Meanwhile, GEN improved the ovarian secretion function of PCOS mice and attenuated oxidative damage of the ovary through enhancing its antioxidant capability dependent on ER. Supplementation of GEN improved the defect of the ATP level and mitochondrial membrane potential, indicating the significance of GEN in preventing mitochondrial dysfunction. Further analysis demonstrated that GEN via ER heightened the expression of Nrf2 and Foxo1 whose blockage antagonized the defence of GEN on the secretory and mitochondrial functions of ovarian granulosa cells followed by the limited antioxidant capability and increased intracellular ROS level. Moreover, nuclear translocation and transcriptional activity of Nrf2 presented a notable enhancement after exposure to GEN. Addition of the Nrf2 inhibitor ML385 hampered the GEN induction of Foxo1. Nrf2 might directly bind to the antioxidant response element of the Foxo1 promoter region. Collectively, GEN might exhibit therapeutic potential for PCOS mice via the ER-Nrf2-Foxo1-ROS pathway.

9.
J Agric Food Chem ; 69(35): 10379-10393, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34436879

RESUMO

α-Dicarbonyls (α-DCs) are key reactive Maillard intermediates with structural diversity and are widely found in foods and in vivo, but little is known regarding the complete molecular profiles of these potentially harmful electrophiles. Herein, we reported a novel isotope-coding derivatization (ICD) strategy for the broad-spectrum, quantitative profiling of (non)target α-DC species in natural foodstuffs. It utilized differential isotope labeling (DIOL) with a reagent pair o-phenylenediamine (OPD)/OPD-d4 (deuterated) to form stable quinoxalines for class-specific fragmentation-dependent acquisition using liquid chromatography-hybrid quadrupole linear ion trap mass spectrometry (LC-QqLIT). A combination of facile one-pot quantitative labeling and convenient cleanup protocol afforded satisfactory sensitivity, linearity, accuracy (81-116%), and process recovery (86-109% with RSDs < 10%) by matrix-matched ICD-internal standard calibration, without significant matrix interference (-9 to 5%), isotopic effect (<0.5%), and cocktail effect. A more generic DIOL-based LC-QqLIT algorithm integrated double precursor ion and neutral loss scan to trigger enhanced product ions with the unique isobaric doublet tags (4 Da shift), enabling simultaneous screening and relative quantitation of nontarget α-DC analogues in a single analysis. This study has widened the vision on complex α-DC profiles in traditional botanicals, which revealed a wide occurrence of α-DCs in such processed sugar-rich products, yet their abundance varied greatly among different samples.


Assuntos
Isótopos , Espectrometria de Massas em Tandem , Cromatografia Líquida , Marcação por Isótopo
10.
Int J Mol Sci ; 22(16)2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34445669

RESUMO

Coronavirus Disease 2019 (COVID-19) remains a global health crisis, despite the development and success of vaccines in certain countries. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, uses its spike protein to bind to the human cell surface receptor angiotensin-converting enzyme 2 (ACE2), which allows the virus to enter the human body. Using our unique cell screening technology, we identified two ACE2-binding peptoid compounds and developed dimeric derivatives (ACE2P1D1 and ACE2P2D1) that effectively blocked spike protein-ACE2 interaction, resulting in the inhibition of SARS-CoV-2 pseudovirus entry into human cells. ACE2P1D1 and ACE2P2D1 also blocked infection by a D614G mutant pseudovirus. More importantly, these compounds do not decrease ACE2 expression nor its enzyme activity (which is important in normal blood pressure regulation), suggesting safe applicability in humans.


Assuntos
Enzima de Conversão de Angiotensina 2/metabolismo , COVID-19/prevenção & controle , Peptidil Dipeptidase A/metabolismo , Peptoides/farmacologia , SARS-CoV-2/efeitos dos fármacos , Internalização do Vírus/efeitos dos fármacos , COVID-19/tratamento farmacológico , COVID-19/virologia , Humanos , Células MCF-7 , Peptoides/metabolismo , Ligação Proteica/efeitos dos fármacos , SARS-CoV-2/metabolismo , SARS-CoV-2/fisiologia , Glicoproteína da Espícula de Coronavírus/metabolismo
11.
Sci Prog ; 104(3): 368504211039363, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34463585

RESUMO

The blood pump is an implantable device with strict performance requirements. Any effective structural improvement will help to improve the treatment of patients. However, the research of blood pump structure improvement is a complex optimization problem with multiple parameters and objectives. This study takes the splitter blade as the object of structural improvement. Computational fluid mechanics and neural networks are combined in research and optimization. And hydraulic experiments and micro particle image velocimetry technology were used. In the optimization study, the number of blades, axial length and circumferential offset are optimization parameters, and hydraulic performance and hemolytic prediction index are optimization targets. The study analyzes the influence of each parameter on performance and completes the optimization of the parameters. In the results, the optimal parameters of number of blades, axial length ratio, and circumferential offset are 2.6° and 0.41°, respectively. Under optimized parameters, hydraulic performance can be significantly improved. And the results of hemolysis prediction and micro particle image velocimetry experiments reflect that there is no increase in the risk of hemolytic damage. The results of this study provide a method and ideas for improving the structure of the axial spiral blade blood pump. The established optimization method can be effectively applied to the design and research of axial spiral blade blood pumps with complex, high precision, and multiple parameters and targets.

12.
J Clin Invest ; 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34464351

RESUMO

The heterogeneity of human hematopoietic stem (HSCs) and progenitor cells (HPCs) under stress conditions such as ex vivo expansion is poorly understood. Here we report that the frequencies of SCID repopulating cells (SRCs) were greatly decreased in cord blood(CB)CD34+ HSCs and HPCs upon ex vivo culture. Transcriptome analysis and metabolic profiling demonstrated that mitochondrial oxidative stress of human CB HSCs and HPCs notably increased along with loss of stemness. Limiting dilution analysis (LDA) revealed that functional human HSCs were enriched in cell populations with low levels of mitochondrial reactive oxygen species (mitoROS) during ex vivo culture. Using single cell RNA sequencing (scRNA-seq) analysis of mitoROS low cell population, we demonstrated that functional HSCs were substantially enriched in the Adhesion G protein-coupled receptor G1 positive (ADGRG1+) population of CD34+CD133+ CB cells upon ex vivo expansion stress. GSEA analysis revealed that HSC signature genes including MSI2 and MLLT3 are enriched in CD34+CD133+ ADGRG1+ CB HSCs. Our study reveals that ADGRG1 enriches for functional human HSCs under oxidative stress during ex vivo culture, which can be a reliable target for drug screening of agonists of HSC expansion.

13.
Fitoterapia ; : 104970, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34419561

RESUMO

The natural product Huperzine A isolated from Huperzia serrata is a targeted inhibitor of acetylcholinesterase that has been approved for clinical use in the treatment of Alzheimer's disease. Given the large demand for natural sources of Huperzine A, efforts have been made to explore whether Huperzine A (Hup. A) is also produced by endophytic fungi from H. serrata and, if so, identify its biosynthetic pathway. These studies have indicated that endophytic fungi from H. serrata represent a huge and largely untapped resource for natural products (including Hup. A) with chemical structures that have been optimized by evolution for biological and ecological relevance. To date, more than three hundred endophytic fungi have been isolated from H. serrata, of which 9 strains can produce Hup. A, whilst more than 20 strains produce other important metabolites, such as polyketones, xanthones, alkaloids, steroids, triterpenoids, furanone derivatives, tremulane sesquitepenes and diterpenoids. In total, 200 secondary metabolites have been characterized in endophytic fungi from H. serrata to date. Functionally, some have cholinesterase-inhibitory or antibacterial activity. This review also considers the different classes of secondary metabolites produced by endophytic fungi, along with their possible applications. We systematically describe the taxonomy, biology, and chemistry of these secondary metabolites. It also summarizes the biosynthetic synthesis of metabolites, including that of Hup. A. The review will aid researchers in obtaining a clearer understanding of this plant-endophyte relationship to better exploit the excellent resources it offers that may be utilized by pharmaceutical industries.

14.
Food Chem ; 365: 130389, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34256228

RESUMO

Leafy vegetables have high nutritional value and are very popular in China. However, the long-term variation in residues and integrated risks of synthetic pyrethroids (SPs) in these vegetables have not been well examined. In this study, a total of 1005 samples were collected from 55 markets during 2017-2019. The cumulative exposure to nine kinds of SPs in Zhejiang, China, through the consumption of nine leafy vegetables was analyzed, and the potential integrated risk was assessed by the relative potency factor. A total of 191 samples were detected with SPs residues. The most frequently detected SPs were λ-cyhalothrin and cypermethrin. The integrated risk assessment results revealed that the dietary risk for these SPs via leafy vegetable exposure is acceptable for children, adults and elderly individuals. The data provided here will be helpful for the government to formulate food policies in China.


Assuntos
Piretrinas , Verduras , Idoso , Criança , China , Contaminação de Alimentos/análise , Humanos , Folhas de Planta , Medição de Risco
15.
Ying Yong Sheng Tai Xue Bao ; 32(7): 2525-2533, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34313071

RESUMO

Based on the distribution data of Tricholoma matsutake obtained from field investigation and literature, the ecological-niche factor analysis (ENFA) and the maximum entropy model (MaxEnt) were used to simulate the distribution law and suitable area of T. matsutake in the western Sichuan Plateau. The prediction was made for the future changes in the suitable area of T. matsutake by analyzing the relationship between climate factors and dynamic distribution. The results showed that the area under curve (AUC) values of both the model training set and validation set were greater than 0.90, indicating that the model prediction results were extremely accurate. The environmental variables affecting the potential distribution of T. matsutake were mainly the lowest temperature in the coldest month, the coldest season precipitation, annual temperature difference and soil type, with accumulative contribution of 90.3%. The niche parameters of suitable distribution areas of T. matsutake were as follows: the lowest temperature in the coldest month was -18.5--5.4 ℃, the coldest season precipitation was less than 15.7 mm, the annual temperature difference was 39.5-45 ℃, and soil type was semi-leached soil, including dry red soil, cinnamon soil, gray cinnamon soil, black soil and grey forest soil. The suitable areas of T. matsutake were distributed in the southwest, south, central and east of the plateau at an altitude range of 1900-3600 m. The highly suita-ble areas were mainly distributed in some towns of Yajiang, Xiangcheng, Kangding, Jiulong, Daocheng, Litang, Batang, Danba, Maerkang, Xiaojin, Jinchuan, Lixian, Maoxian, etc. The moderately and lowly suitable areas were located in some towns of Derong, Daofu, Xinlong, Luhuo, Baiyu, Luding, Rangtang, Wenchuan, Heishui, Jiuzhaigou. The highly suitable areas were discontinuously distributed according to the direction of rivers and mountains. The moderately suitable areas were connected with the highly suitable areas, while the lowly suitable areas were the extension of the highly and moderately suitable areas. Future climate change would be beneficial to the growth of T. matsutake on the western Sichuan Plateau, while the climate-suitable areas would show an overall increasing trend. Suitable areas in the low-altitude Minjiang River Basin would be more affected by climate change than those located in high-altitude areas.


Assuntos
Tricholoma , Agaricales , China , Mudança Climática , Ecossistema
16.
J Sex Med ; 18(9): 1632-1640, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34247955

RESUMO

BACKGROUND: The number of individuals with potential gender dysphoria (GD) being referred to specialized gender identity clinics or programs is increasing internationally; these cases are initially screened using the Gender Identity/Gender Dysphoria Questionnaire for Adolescents and Adults (GIDYQ-AA). AIM: The current study aimed to assess the psychometric properties of the GIDYQ-AA in a sample of adolescents and young adults from China. METHODS: A cross-sectional study was conducted in October 2020. Sociodemographic information of the participants was first collected. Participants then completed the GIDYQ-AA, the Generalized Anxiety Disorder-7 scale, the Patient Health Questionnaire-9, and a suicidal ideation assessment. A total of 2,533 participants with a mean age of 19.30 (SD = 1.19) years were recruited. Of the participants, 841 (33.2%) were cis men, 1,589 (62.7%) were cis women, 66 (2.6%) self-identified as transgender, 17 (0.7%) self-identified as non-binary, and 20 (0.8%) self-identified as gender queer. RESULTS: The GIDYQ-AA had high internal consistency with a Cronbach's alpha = 0.89. Exploratory factor analysis showed that the GIDYQ-AA had a four-factor structure in China. The GIDYQ-AA was significantly correlated with anxiety symptoms (r = -0.32, P < .01), depressive symptoms (r = -0.33, P < .01), and suicidal ideation (r = -0.20, P < .01). CLINICAL TRANSLATION: The Chinese version of GIDYQ-AA is a useful measurement with high practical value, which could promote the assessment and research of GD across China or among Chinese migrants in other countries. STRENGTHS AND LIMITATIONS: This is the first study assessing the psychometric properties of the GIDYQ-AA in Chinese adolescents and young adults. The convergent and divergent validity of the GIDYQ-AA were not examined due to the unavailability of data. Also, the sample did not have an equal distribution of male to males and female to females. CONCLUSION: The Chinese version of GIDYQ-AA is a useful measure, which could promote both the assessment and research of GD in the Chinese population. Wang Y, Feng Y, Su D, et al. Validation of the Chinese Version of the Gender Identity/Gender Dysphoria Questionnaire for Adolescents and Adults. J Sex Med 2021;18:1632-1640.


Assuntos
Disforia de Gênero , Adolescente , Adulto , China , Estudos Transversais , Feminino , Disforia de Gênero/diagnóstico , Identidade de Gênero , Humanos , Masculino , Psicometria , Reprodutibilidade dos Testes , Inquéritos e Questionários , Adulto Jovem
17.
J Biol Chem ; 297(2): 100933, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34216622

RESUMO

Precursor messenger RNA (pre-mRNA) splicing is critical for cell growth and development, and errors in RNA splicing frequently cause cellular dysfunction, abnormal gene expression, and a variety of human diseases. However, there is currently a lack of reliable systems to noninvasively monitor the mRNA splicing efficiency in cells and animals. Here, we described the design of a genetically engineered ratiometric dual luciferase reporter to continuously quantify the changes in mRNA splice variants in vivo. This reporter system is encoded within a single polypeptide but on separate exons, thus generating two distinct luciferase signals derived from spliced and unspliced mRNAs. With this reporter, the two kinds of luciferase in the same individual can minimize the influence of indirect factors on splicing, and the ratio of these two luciferase intensities represents the dynamic splicing efficiency of pre-mRNA. Our study offers a convenient and robust tool for the screening and identification of small molecules or trans-acting factors that affect the efficiency of specific splicing reactions.

18.
J Clin Neurosci ; 90: 217-224, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34275553

RESUMO

Postoperative delirium (POD) is a significant clinical problem in neurosurgical patients after intracranial surgery. Identification of high-risk patients may optimize perioperative management, but an adequate risk model for use at early phase after operation has not been developed. In the secondary analysis of a prospective cohort study, 800 adult patients admitted to the ICU after elective intracranial surgeries were included. The POD was diagnosed as Confusion Assessment Method for the ICU positive on postoperative day 1 to 3. Multivariate logistic regression analysis was used to develop early prediction model (E-PREPOD-NS) and the final model was validated with 200 bootstrap samples. The incidence of POD in this cohort was19.6%. We identified nine variables independently associated with POD in the final model: advanced age (OR 3.336, CI 1.765-6.305, 1 point), low education level (OR 2.528, 1.446-4.419, 1), smoking history (OR 2.582, 1.611-4.140, 1), diabetes (OR 2.541, 1.201-5.377, 1), supra-tentorial lesions (OR 3.424, 2.021-5.802, 1), anesthesia duration > 360 min (OR 1.686, 1.062-2.674, 0.5), GCS < 9 at ICU admission (OR 6.059, 3.789-9.690, 1.5), metabolic acidosis (OR 13.903, 6.248-30.938, 2.5), and neurosurgical drainage tube (OR 1.924, 1.132-3.269, 0.5). The area under the receiver operator curve (AUROC) of the risk score for prediction of POD was 0.865 (95% CI 0.835-0.895). The AUROC was 0.851 after internal validation (95% CI 0.791-0.912). The model showed good calibration. The E-PREPOD-NS model can predict POD in patients admitted to the ICU after elective intracranial surgery with good accuracy. External validation is needed in the future.


Assuntos
Craniotomia/efeitos adversos , Delírio do Despertar/diagnóstico , Fatores de Risco , Adulto , Idoso , Estudos de Coortes , Procedimentos Cirúrgicos Eletivos/efeitos adversos , Delírio do Despertar/epidemiologia , Feminino , Humanos , Incidência , Unidades de Terapia Intensiva , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
19.
J Autism Dev Disord ; 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34264487

RESUMO

Interoception is believed to underlie socio-cognitive functions which are often impaired in individuals with autism spectrum disorders (ASD). In this study, Experiment 1 examined in a sample of 114 neurotypical college students the associations among autistic traits, alexithymia, and interoceptive accuracy (IA), which was assessed by a novel paradigm "Eye-tracking Interoceptive Accuracy Task (EIAT). In Experiment 2, EIAT and the Autism Spectrum Quotient were administered to 52 preschool children, 50 adolescents, and 50 adults. Experiment 1 supported the association between autistic traits and alexithymia, but failed to support their association with multidimensional interoception. Experiment 2 strongly supported the association between age and IA. We conclude that cardiac IA differs between neurotypical age groups, but shows limited relationship with autistic traits.

20.
PLoS One ; 16(7): e0254502, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34255775

RESUMO

Planting soybeans (Glycine max (L.) Merr.) in tea gardens decreased soil pH in theory but increased it in practice. This controversy was addressed in this study by treating the tea garden soil consecutively with different parts of a soybean cover crop: aboveground soybean (ASB) parts, underground soybean (USB) root residues, and the whole soybean (WSB) plants. In comparison with the control, the soil pH increased significantly after the third ASB and WSB treatments, but there was no significant change in the soil pH in the USB treatment. Concordantly, the soil exchangeable acidity decreased significantly and the soil exchangeable bases increased significantly in the ASB and WSB treatments. The exchangeable acidity increased in the USB treatment, but the amount of the increased acidity was less than that of the increased bases in the ASB treatment, resulting in a net increase in the exchangeable bases in the WSB treatment. Soybean planting and covering also increased the microbial richness and abundance significantly, which led to significantly more soil organic matters. Exchangeable K+ and Mg2+, and soil organic matters played significantly positive roles and exchangeable Al3+ played negative roles in improving soil pH. Our data suggest that consecutive plantings of soybean cover crop increase the pH of the acidified tea garden soil.

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