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1.
J Colloid Interface Sci ; 607(Pt 2): 1391-1401, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34583044

RESUMO

Photocatalytic water splitting for hydrogen production is an important strategy to achieve clean energy development. In this report, a novel three-dimensional (3D) hierarchical hollow tubular g-C3N4/ZnIn2S4 nanosheets (HTCN/ZIS) type-Ⅱ heterojunction photocatalyst was successfully prepared and applied for photocatalytic hydrogen production under visible light irradiation. The experimental results reveal that the optimal proportion of HTCN/ZIS with the remarkable photocatalytic H2 evolution rate of 20738 µmol h-1 g-1 was obtained. The main reasons for the improvement of hydrogen production activity are as follows: (i) this unique tubular hollow structure can effectively enhances the light capturing ability by the multiple light scattering/reflection of incident light in the inner cavity; (ii) the shorten the phase plane transmission distance could reduce the path of charge transfer; (iii) the surface coated a large number of scaly ZnIn2S4 nanosheets can provide abundant reactive sites. Combining the various characterization tests, the enhanced spatial segregation of charge carriers could owning to the intimately interfacial contact and well-matched band gaps structure between g-C3N4 and ZnIn2S4 through the type-II heterojunction. This work provides a new prospect for the construction of a novel 3D hierarchical type-II heterojunction photocatalyst for highly efficient photocatalytic hydrogen production.

2.
Cancer Lett ; 525: 179-197, 2022 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-34752845

RESUMO

The transient receptor potential melastatin-subfamily member 7 (TRPM7) is a ubiquitous cation channel possessing kinase activity. TRPM7 mediates a variety of physiological responses by conducting flow of cations such as Ca2+, Mg2+, and Zn2+. Here, we show that the activation of TRPM7 channel stimulated by chemical agonists of TRPM7, Clozapine or Naltriben, inhibited autophagy via mediating Zn2+ release to the cytosol, presumably from the intracellular Zn2+-accumulating vesicles where TRPM7 localizes. Zn2+ release following the activation of TRPM7 disrupted the fusion between autophagosomes and lysosomes by disturbing the interaction between Sxt17 and VAMP8 which determines fusion status of autophagosomes and lysosomes. Ultimately, the disrupted fusion resulting from stimulation of TRPM7 channels arrested autophagy. Functionally, we demonstrate that the autophagy inhibition mediated by TRPM7 triggered cell death and suppressed metastasis of cancer cells in vitro, more importantly, restricted tumor growth and metastasis in vivo, by evoking apoptosis, cell cycle arrest, and reactive oxygen species (ROS) elevation. These findings represent a strategy for stimulating TRPM7 to combat cancer.

3.
Chemosphere ; 287(Pt 3): 132284, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34563782

RESUMO

Among polychlorinated naphthalenes (PCNs), listed by the Stockholm convention as Persistent Organic Pollutants (POPs), hexachloronaphthalenes are considered the most toxic and raise the highest concern. Of these, 1,2,3,5,6,7-hexachloronaphthalanene (PCN67) is considered the main congener affecting human health due to its hepatotoxicity and its ability to disturb the reproductive, endocrine, and hematological systems. It is also prevalent in human serum/plasma, milk, and adipose tissue. However, little is known about its neurotoxicity, despite the fact that anorectic effects have been observed in workers occupationally exposed to PCNs and in animal research on PCN67. Since dopamine is involved in many aspects of food intake, the aim of this study was to confirm whether PCN67 affects dopamine synthesis in differentiated PC12 cells, a widely used model of neurosecretion. Our results show that exposure to PCN67 resulted in diminished dopamine content and release. Moreover, PCN67 also affected the expression of tyrosine hydroxylase and lowered the expression of vesicular monoamine transporter 1 (VMAT1). In addition, significantly lower expression of antioxidant enzymes, including catalase, glutathione peroxidase and copper/zinc superoxide dismutase, was observed in comparison to the vehicle. In conclusion, PCN67 appears to disturb dopaminergic transmission by altering tyrosine hydroxylation, reducing VMAT1 expression and impairing antioxidant protection. Our study provides a potential mechanism for how PCN67 may cause dopamine deficiency and contribute to neuronal death by affecting cellular antioxidant potency; however, this conclusion requires further research.


Assuntos
Dopamina , Síndromes Neurotóxicas , Animais , Humanos , Naftalenos/toxicidade , Células PC12 , Ratos
4.
Sci Total Environ ; 806(Pt 3): 151390, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34740654

RESUMO

Antibiotic resistance gene (ARG) pollution in estuarine environment has drawn great attention, and it is not clear if the physical and chemical parameters such as salinity, total organic carbon, total nitrogen, total phosphorus and antibiotics affects the distribution of ARGs. Herein, we deciphered the ARG profiles and microbial community compositions in sediments from Jiulong River Estuary (JRE) and Min River Estuary (MRE) of China using high-throughput sequencing-based metagenomics analysis. Furthermore, we explored the influence of salinity on bacterial community and ARG profiles. The results showed that Proteobacteria, Chloroflexi, and Acidobacteria were the dominant phyla in these two estuaries. The abundance of ARGs ranged from 1.05 × 10-1 to 2.93 × 10-1 copy of ARG per copy of 16S rRNA gene in all the sediment samples and the profiles of ARGs presented similar patterns in two estuaries. Multidrug resistance genes were the dominant ARG types in both estuaries, with an overall abundance of 2.39 × 10-2-1.07 × 10-1 copy of ARG per copy of 16S rRNA gene, followed by genes conferring resistance to bacitracin and macrolide-lincosamide-streptogramin. Salinity was an important influencing factor on the bacterial community but not on the ARG profiles. Instead, stochastic processes exerted the main influence on the distribution of ARGs. The comparison of ARG profiles among estuary sediments, marine sediments, and samples from anthropogenic pollution environments revealed remarkable similarity of ARG profiles between samples from estuary sediments and those from municipal wastewater treatment plants. These results suggested that the complex emission of anthropogenic pollution could cause the stochastic ecological pattern of ARGs.

5.
Methods Mol Biol ; 2377: 423-430, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34709630

RESUMO

Computational tool composites alternative way to identify essential genes and it is low-cost and time-efficient. Based on experimental essentiality sets deposited in the databases DEG and OGEE as reference, we developed an automatically computational tool named Geptop to select essential genes from the set of protein-coding genes in a prokaryotic genome, which utilizes the strategy of reciprocally best hit for homology search and evolutionary distance for weight assigning. The latest version of Geptop is 2.0 ( http://guolab.whu.edu.cn/geptop ), which can predict gene essentiality with the mean AUC 0f 0.84 in prokaryotes and is more stable. The chapter is to briefly introduce the tool and tell how to use it.

6.
J Int Med Res ; 49(11): 3000605211058355, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34851773

RESUMO

Untreated individuals with antithrombin (AT) deficiency are at higher risk of thrombosis and adverse pregnancy outcomes. The present recommendations are mostly empirical for treating patients with AT deficiency during pregnancy because of the absence of guidelines. We report a rare case of heparin resistance due to AT deficiency in a pregnant 32-year-old Chinese woman. We also reviewed the English medical literature for AT deficiency and its association with thromboembolism and treatment. This patient suffered two early miscarriages because of thrombosis due to AT deficiency. The patient was administered the combination of adequate low molecular weight heparin with fresh frozen plasma and warfarin because of her heparin resistance. She delivered a healthy female newborn without any adverse effects of the anticoagulation therapy. Our findings suggest that the combination of adequate low molecular weight heparin with fresh frozen plasma and warfarin is effective for preventing thrombus during pregnancy.

7.
Rev Sci Instrum ; 92(11): 115003, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34852548

RESUMO

This paper proposes a synchronous demodulation technology based on sample-and-hold which shows significant advantages in the application of precision sensors. The traditional synchronous demodulation methods are discussed, and then the working principle of the proposed method is theoretically analyzed in detail. It is found that the proposed method can not only effectively suppress the harmonic components caused by the signal source but is also beneficial to improving the dynamic range of precision sensors. The eddy current sensor is adopted as an example of precision sensors, and a high-precision eddy current displacement sensor prototype was designed and tested. The results show that with the proposed method, the harmonics in the output signal are suppressed more effectively, and the demodulation circuit is relatively simplified. This synchronous demodulation method has an extensive application prospect in precision sensors.

8.
Mol Oncol ; 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34854226

RESUMO

Poly [ADP-ribose] polymerase (PARP) inhibitors can block DNA single-strand damage repair and subsequently increase double-stranded breaks (DSBs) by reducing the activity of the PARP1 protease and preventing the PARP1 protein from dissociating from chromatin. Tumors with the BRCA mutation are particularly sensitive to PARP inhibitors. So far, PARP inhibitors (olaparib) have been used to treat pancreatic cancer patients with BRCA mutation. However, these patients are prone to PARP-inhibitor resistance. Our previous studies suggest that fructose-1,6-bisphosphatase 1 (FBP1) is responsible for the sensitivity to various anticancer agents, such as gemcitabine or mitogen-activated protein kinase kinase (MEK) inhibitors. In this study, we demonstrate that FBP1 regulates the sensitivity to PARP inhibitors in pancreatic cancer. Then, we showed that nuclear FBP1 is responsible for this process by interacting with DNA (cytosine-5)-methyltransferase 1 (DNMT1) and trapping PARP1 in chromatin. Moreover, we revealed that ubiquitin carboxyl-terminal hydrolase 7 (USP7) binds to and induces the deubiquitination of FBP1, which prevented FBP1 from translocating to the nucleus. Finally, we demonstrated that USP7 inhibitors enhanced the antitumor effect of PARP inhibitors in an FBP1-dependent manner. Collectively, our results identify a novel USP7-FBP1-DNMT1 signaling axis in pancreatic cancer, which might indicate that USP7 inhibitors and PARP inhibitors might have more powerful antitumor effects than PARP inhibitors alone in pancreatic cancer patients.

9.
J Cancer Res Ther ; 17(5): 1281-1285, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34850779

RESUMO

Objectives: Wilms tumor is a common pediatric malignant tumor that accounts for approximately 95% of kidney tumors in children. The role of lipid metabolism in tumors has attracted increased attention in recent years. We examined the role of hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), a lipid metabolism enzyme, in the pathogenesis of Wilms tumor. Materials and Methods: In a previous study, we screened Wilms tumors and adjacent normal tissues for differentially expressed proteins by mass spectrometry and verified the results by western blot analysis. The Oncomine database and quantitative reverse transcription-polymerase chain reaction were used to verify the expression of HADHA at the genetic level. Immunohistochemistry and immunofluorescence were also used to validate the differential expression of the HADHA protein. The relationship between histopathological typing, clinical pathology, and HADHA expression was analyzed in 65 paraffin-embedded specimens from pediatric Wilms tumor patients. Kaplan-Meier survival curves were used to analyze the relationship between the expression of HADHA and patient prognosis. Results: HADHA was expressed at low levels in Wilms tumor tissue compared with the corresponding normal tissue. The expression of HADHA was closely associated with histopathological typing (P = 0.030). The prognostic analysis of 65 children with Wilms tumor showed that high expression of HADHA was closely associated with poor prognosis (P = 0.046). Conclusions: HADHA expression is downregulated in Wilms tumor tissues, but high expression in tumor tissues is associated with clinical stage and the prognosis of children with this tumor.

10.
Insect Biochem Mol Biol ; : 103698, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34848284

RESUMO

Histamine-gated chloride channels (HACls) mediate fast inhibitory neurotransmission in invertebrate nervous systems and have important roles in light reception, color processing, temperature preference and light-dark cycle. The fall armyworm, Spodoptera frugiperda is a main destructive pest of grain and row crops. However, the pharmacological characterization of HACls in S. frugiperda remain unknown. In this study, we identified two cDNAs encoding SfHACl1 and SfHACl2 in S. frugiperda. They had similar expression patterns and were most abundantly expressed in the head of larvae and at the egg stage. Electrophysiological analysis with the two-electrode voltage clamp method showed that histamine (HA) and γ-aminobutyric acid (GABA) activated inward currents when SfHACls were singly or collectively expressed with different ratios in Xenopus laevis oocytes. These channels were ≥2000-fold more sensitive to HA than to GABA. They were anion-selective channels, which were highly dependent on changes in external chloride concentrations, but insensitive to changes in external sodium concentrations. The insecticides abamectin (ABM) and emamectin benzoate (EB) also activated these channels with the EC50 to SfHACl1 lower than that to SfHACl2. And the EC50s of ABM and EB to the co-expressed channels gradually increased with increase in the injection ratio of SfHACl2 cRNA. Homology models and docking simulations revealed that HA bound to the large amino-terminal extracellular domain of SfHACl1 and SfHACl2 by forming 4 and 2 hydrogen bonds, respectively. The docking simulations of ABM and EB had similar binding sites in the transmembrane regions. Overall, these findings indicated that HACls act as targets for macrolide, and this study provides theoretical guidance for further derivatization of abamectin insecticides.

11.
Chemosphere ; : 132963, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34800508

RESUMO

Microbial fuel cells (MFCs) are of great interest due to their capability to directly convert organic compounds to electric energy. In particular, MFCs technology showed great potential to directly harness the energy from xylose in the form of bioelectricity and biohydrogen simultaneously. Herein, we report a yeast strain of Cystobasidium slooffiae JSUX1 enabled the reduction and assembly of graphene oxide (GO) nanosheets into three-dimensional reduced GO (3D rGO) hydrogels on the surface of carbon felt (CF) anode. The autonomously self-modified 3D rGO hydrogel anode entitled the yeast-based MFCs with two times enhancement on bioelectricity and biohydrogen production from xylose. Further analysis demonstrated that the 3D rGO hydrogel attracted more yeast cells and reduced the interfacial charge transfer resistance, which was the underlying mechanism for the improvement of MFCs performance. This work offers a new strategy to reinforce the performance of yeast-based MFCs and provides a new opportunity to efficiently harvest energy from xylose.

13.
Interdiscip Sci ; 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34817803

RESUMO

In 2002, our research group observed a gene clustering pattern based on the base frequency of A versus T at the second codon position in the genome of Vibrio cholera and found that the functional category distribution of genes in the two clusters was different. With the availability of a large number of sequenced genomes, we performed a systematic investigation of A2-T2 distribution and found that 2694 out of 2764 prokaryotic genomes have an optimal clustering number of two, indicating a consistent pattern. Analysis of the functional categories of the coding genes in each cluster in 1483 prokaryotic genomes indicated, that 99.33% of the genomes exhibited a significant difference (p < 0.01) in function distribution between the two clusters. Specifically, functional category P was overrepresented in the small cluster of 98.65% of genomes, whereas categories J, K, and L were overrepresented in the larger cluster of over 98.52% of genomes. Lineage analysis uncovered that these preferences appear consistently across all phyla. Overall, our work revealed an almost universal clustering pattern based on the relative frequency of A2 versus T2 and its role in functional category preference. These findings will promote the understanding of the rationality of theoretical prediction of functional classes of genes from their nucleotide sequences and how protein function is determined by DNA sequence.

14.
Phys Chem Chem Phys ; 23(46): 26343-26348, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34788775

RESUMO

Valence Compton profiles (CPs) (electron momentum density projections) of B-doped carbon nano-onions (CNOs) as a function of the boron doping content were obtained by recording electron energy-loss spectra at large scattering angles using a transmission electron microscope, a technique known as electron Compton scattering from solids (ECOSS). The amplitude of the CPs at zero momentum increases with increasing doping content, while the shape of the CPs becomes narrower with increasing doping content. The differences between the profiles of B-doped CNOs and that of pristine CNOs have been clearly observed. These experimental results indicate substantially greater delocalization of the ground-state charge density in B-doped CNOs than in pristine CNOs. The results clearly demonstrate that the ECOSS technique is an efficient and reliable experimental method for studying electron density distributions in solids as a function of the heteroatom doping content.

15.
Accid Anal Prev ; 164: 106502, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34837850

RESUMO

The rear-end crash is one of the most common types of crashes, and key risk factors have been broadly identified in the car-following behaviors preceding a crash. However, the relationships between rear-end crash risk and daily car-following behaviors, or habits, have not been well examined. This study aims to identify the daily car-following behaviors on urban surface roads and urban expressways that have the most influence on rear-end crashes and near-crashes (CNC). Two months of naturalistic driving study data were used to investigate the daily car-following behavior of 54 drivers. A paired t-test and a Wilcoxon matched-pairs signed rank test were conducted to find the differences in behaviors on the two road types, and basic Poisson regression and Poisson hurdle regression models were used to explore significant risk factors. Results revealed that (1) drivers' longitudinal vehicle control, time control, and emergency behaviors are significantly different on urban surface roads and urban expressways; (2) for surface roads, three key influencing factors were ranked, in descending order, as the standard deviation of relative speed, percentage of time gap less than 1 s, and maximum acceleration; (3) for expressways, four key factors were ranked: minimum time gap, maximum deceleration, percentage of TTC less than 5 s, and the percentage of large positive jerk. The knowledge achieved on risky daily driving behaviors can be applied to training drivers to improve safe practices, assist insurance companies in creating usage-based insurance strategies, and support driver assistant systems design.

17.
Nat Biomed Eng ; 5(11): 1320-1335, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34725507

RESUMO

In breast cancer, genetic heterogeneity, the lack of actionable targets and immune evasion all contribute to the limited clinical response rates to immune checkpoint blockade therapy. Here, we report a high-throughput screen based on the functional interaction of mouse- or patient-derived breast tumour organoids and tumour-specific cytotoxic T cells for the identification of epigenetic inhibitors that promote antigen presentation and potentiate T-cell-mediated cytotoxicity. We show that the epigenetic inhibitors GSK-LSD1, CUDC-101 and BML-210, identified by the screen, display antitumour activities in orthotopic mammary tumours in mice, that they upregulate antigen presentation mediated by the major histocompatibility complex class I on breast tumour cells and that treatment with BML-210 substantially sensitized breast tumours to the inhibitor of the checkpoint programmed death-1. Standardized measurements of tumour-cell killing activity facilitated by tumour-organoid-T-cell screens may help with the identification of candidate immunotherapeutics for a range of cancers.

18.
J Fungi (Basel) ; 7(11)2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34829241

RESUMO

Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host's immune system to avoid being attacked. Sporisorium scitamineum is the causative agent of sugarcane smut, the most important disease in sugarcane-producing regions worldwide. In this work, we functionally characterized the conserved effector PEP1 in S. scitamineum. The mating process and the expression of genes in the MAPK signaling pathway and the a and b loci were adversely affected in Sspep1-null mutants. The requirement for SsPEP1 in pathogenicity and symptom development was allele dosage-dependent, i.e., deleting one Sspep1 allele in the mating pair turned a normal black whip with abundant teliospores into a white whip with few teliospores; however, deleting both alleles almost abolished infectivity and whip development. ΔSspep1 mutants produced significantly less mycelium mass within infected plants. Additionally, SsPEP1 was identified as a potent inhibitor of sugarcane POD-1a peroxidase activity, implying that SsPEP1 may function to relieve reactive oxygen species-related stress within the host plant. Taken together, our work demonstrated that SsPEP1 is a multifaceted effector essential for S. scitamineum growth, development, and pathogenicity.

19.
Int J Mol Sci ; 22(22)2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34830111

RESUMO

Although some breast cancer patients die due to tumor metastasis rather than from the primary tumor, the molecular mechanism of metastasis remains unclear. Therefore, it is necessary to inhibit breast cancer metastasis during cancer treatment. In this case, after designing and synthesizing CTI-2, we found that CTI-2 treatment significantly reduced breast cancer cell metastasis in vivo and in vitro. Notably, with the treatment of CTI-2 in breast cancer cells, the expression level of E-cadherin increased, while the expression level of N-cadherin and vimentin decreased. In addition, after CTI-2 treatment, those outflow levels for p-ERK, p-p38, and p-JNK diminished, while no significant changes in the expression levels of ERK, JNK, or p38 were observed. Our conclusion suggested that CTI-2 inhibits the epithelial-mesenchymal transition (EMT) of breast carcinoma cells by inhibiting the activation of the mitogen-activated protein kinase (MAPK) signaling pathway, thereby inhibiting the metastasis of breast tumor cells. Therefore, we believe that CTI-2 is another candidate for breast tumor medication.

20.
Zhongguo Gu Shang ; 34(10): 965-70, 2021 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-34726027

RESUMO

OBJECTIVE: To summarize the complications of core drilling intramedullary nail in the treatment of femoral shaft closed fracture and explore the treatment strategy. METHODS: From August 2014 to June 2018, a total of 215 patients with closed femoral shaft fractures were treated with closed reduction core drill intramedullary nail, including 129 males and 86 females, aged from 18 to 62 years, with an average of (44.2±10.6) years old. The time from injury to operation was 3 to 21 days. There were 102 cases of AO type A fracture, 82 cases of AO type B fracture and 31 cases of AO type C fracture. The time of operation, the amount of blood loss during operation, the duration of hospitalization, the time of fracture healing and the HSS score of knee joint function at the last follow-up were recorded. The observation of complications included:iatrogenic fracture, core drill broken, core drill twist, postoperative infection, and fracture nonunion. RESULTS: The average operation time was (63.2± 15.6) min and intraoperative blood loss was (150.0±34.5) ml. All the incisions reached grade A healing. Patients were follow up for a mean of (18.5±3.2) months, the average hospital stay was (4.3±1.2) days, and the average fracture healing time was (5.6±2.3) months. At the final follow-up, the average HSS score of knee joint was 90.3±4.7. Related complications occurred in 37 cases (17.2%). The core drill related complications occurred in 13 cases (6.0%), including core drill broken in 5 cases (2.3%), core removal in 1 case and slotting in 4 cases;core drill twist in 8 cases (3.7%). After the core was cut, the core was removed. Similar complicationsof conventional intramedullary nail:iatrogenic fracture was performed in 12 cases (5.6%), including 10 cases of fracture end split and 2 cases of distal perimedullary fracture of intramedullary nail. The patients with cleavage at the fracture end were not treated after judging their stability, and the patients with fracture around the distal end of the intramedullary nail were fixed with auxiliary steel plate during operation;1 case(0.4%) with delayed infection after operation, debridement and external fixation was replaced and healed after bone transfer; fracture nonunion occurred in 11 cases (5.1%), of which 7 cases (3.3%) were hypertrophic nonunion and healed with additional plate. Atrophic nonunion occurred in 4 cases (1.9%), which healed after additional steel plate and bone graft. CONCLUSION: Core drilling intramedullary nail is an effective method for the treatment of closed femoral shaft fracture, and the complications include core drill related complications and conventional intramedullary nail similar complications. Accurate preoperative evaluation, careful operation during operation and early postoperative symptomatic treatment can effectively reduce the occurrence of related complications.


Assuntos
Fraturas do Fêmur , Fixação Intramedular de Fraturas , Fraturas Fechadas , Adulto , Pinos Ortopédicos , Feminino , Fraturas do Fêmur/cirurgia , Fixação Intramedular de Fraturas/efeitos adversos , Consolidação da Fratura , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
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