Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 690
Filtrar
1.
Talanta ; 206: 120212, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514841

RESUMO

Graphene oxide-Fe3O4 (GO-Fe3O4) nanocomposite was synthesized by a facile chemical co-precipitation method. The GO-Fe3O4 was used as magnetic sorbent to extract the eight psychoactive drugs from urine samples. The analytes are morphine (MOR), 6-monoacetylmorphine (6-MAM), amphetamine (AMP), methamphetamine (MAMP), codeine, cocaine, dolantin and benzoylecgonine (BZE), which were determined by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). This method has high selectivity for the target analytes. The limit of detection (LOD) and limit of quantification (LOQ) were 0.02-0.2 µg L-1 and 0.05-0.5 µg L-1, respectively. The Mandel's fitting test revealed good linearity within all linear ranges. The linear ranges were calculated as 0.05-1000 µg L-1 for AMP, MAMP, cocaine and dolantin; 0.1-1000 µg L-1 for 6-MAM and codein; and 0.5-1000 µg L-1 for MOR and BZE. The recoveries ranged in 80.4-105.5%. The intra-day and inter-day RSDs are in the range of 2.7-13.1% and 3.9-13.7%, respectively. Magnetic solid phase extraction (MSPE) with GO-Fe3O4 provides a convenient, rapid and green sample pretreatment method for extracting the target psychoactive drugs from urine. This methodology can be used for simultaneous or individual detection of eight major psychoactive drugs with high sensitivity. This method has high potential in clinical and forensic areas for psychoactive drugs analysis.

2.
Sci Total Environ ; 698: 134229, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505341

RESUMO

Polycyclic aromatic hydrocarbons (PAHs) in the environment are of significant concerns due to their high toxicity to human health. PAHs measurements at limited air quality monitoring stations alone are insufficient to gain a complete understanding of ambient levels and public exposure of PAHs in China. This study simulated the concentrations of PAHs in China, identified the source contributions, and estimated the health risks. Anthropogenic emissions of 16 priority PAHs directly associated with health risks were generated from the global high-resolution PKU-FUEL-2007 inventory. Open biomass burning emissions were generated from the Fire Inventory from NCAR (FINN). PAHs concentrations in January, April, July, and October 2013 were simulated using the Community Multiscale Air Quality (CMAQ) model after incorporation of chemistry, partitioning, and deposition of PAHs. Predicted PAHs were in good agreement with seasonal and annual averaged observations from previous studies. The surface concentrations of 16-PAHs were higher in winter, with population weight average of 0.8 µg/m3 and peak value of 2.0 µg/m3 in urban areas in the North China Plain (NCP) and the Yangtze River Delta (YRD). Summer and spring exhibited lower concentrations of approximately 0.2 µg/m3 in most areas. The most important sources to PAHs were biomass burning and coal combustion in winter and industrial processes and oil and gas activities in summer. The cancer risk due to inhalation exposure of naphthalene (NAPH) and seven carcinogenic PAHs was significant, with the incremental lifetime cancer risk (ILCR) of >5 × 10-4 in many urban and industrial areas. Exposure to PAHs was estimated to result in 15,198 excess lifetime cancer cases in China. Oil and gas burning associated with transport, residential and commercial activities were major contributors to ILCR in China. Coal combustion was predominant in Shanxi but less important in other regions.

3.
J Photochem Photobiol B ; 202: 111668, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31734435

RESUMO

Fraxinus rhynchophylla belongs to the family of Oleaceae and also called as Chinese ash wood possesses various pharmacological properties such as neuroprotective, antimicrobial, anti-inflammatory, etc. Therefore we synthesized ZnO nanoparticles using Fraxinus rhynchophylla wood extract as reducing and capping agent. The synthesized nanoparticles were characterized with the aid of UV-Spec, DLS, FT-IR and TEM analysis. Green synthesized ZnO nanoparticles were then assessed for anti-nociceptive property by using various nociception models such as thermal stress-induced, acetic acid, glutamate, capsaicin, and formalin-induced nociception. The sedative effect of synthesized ZnO nanoparticles was evaluated with an open field test. UV-Spectroscopic analysis confirms the formation of ZnO nanoparticles and the characterization studies DLS, FT-IR, and TEM analysis prove it has ideal nanoparticle can be used as a nano-drug. Results of both thermal stress-induced methods hot plate and tail immersion nociception test verified the synthesized ZnO nanoparticles are a potent antinociceptive drug. ZnO nanoparticles effectively reduced the abdominal writhes in acetic acid-induced nociception and it also significantly decreased the nociception activity in another glutamate, capsaicin, and formalin-induced nociception models. Open field experiment proved that synthesized ZnO nanoparticles are less sedative compared to the standard antinociceptive drug morphine. Overall our findings authentically confirm ZnO nanoparticles synthesized from Fraxinus rhynchophylla wood extract is a novel drug that persuasively reduces nociception in different nociceptive induced mice models and can be the best alternative for allopathic drugs which renders severe side effects.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31755252

RESUMO

In this paper, a new kind of anti-icing materials: liquid-infused micro-nanostructured MOF coating (LIMNSMC) was designed and prepared. The porous micro-nanostructures of MOF coating were first utilized to immobilize lubricating liquid. The anti-icing performance of LIMNSMC could be tuned by the viscosity, the amount of lubricating liquid and the surface morphology. Under appropriate condition, the LIMNSMC shows high anti-icing performance with the condensed water freezing temperature of approximately -39 ℃ and the ice adhesion strength of approximately 10 kPa, because the micro-nanostructures of MOF coating reduce the contact area and hinder the heat transfer between surface and water droplets, and the lubricating layer effectively reduce the heterogeneous nucleation sites on surface, as well as reduce the ice adhesion. LIMNSMCs exhibit good durability due to the lubricating liquid could be effectively immobilized by the nanopores of MOFs. So the high anti-icing performance of LIMNSMCs could maintain throughout ten freezing/melting cycles and six icing-deicing cycles, and slightly decrease after high speed centrifugation and 50 abrasion cycles.

5.
Front Immunol ; 10: 2408, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31681297

RESUMO

Astrocyte-mediated inflammation and oxidative stress elicit cerebral ischemia-reperfusion (IR) injury after stroke. Nuclear factor (NF)-κB activates astrocytes and generates pro-inflammatory factors. The purpose of the present study is to elucidate the effect of pterostilbene (PTE, a natural stilbene) on astrocytic inflammation and neuronal oxidative injury following cerebral ischemia-reperfusion injury. A middle cerebral artery occlusion-reperfusion (MCAO/R) mouse model and HT22/U251 co-culture model subjected to oxygen-glucose deprivation and re-introduction (OGD/R) were employed, with or without PTE treatment. The data showed that PTE delivery immediately after reperfusion, at 1 h after occlusion, decreased infarct volume, brain edema, and neuronal apoptosis and improved long-term neurological function. PTE decreased oxidation (i.e., production of reactive oxygen species, malondialdehyde) and inflammatory mediators (tumor necrosis factor-α, interleukin-1ß, and interleukin-6) and increased anti-oxidative enzyme activities (i.e., of superoxide dismutase, glutathione peroxidase), by inhibiting phosphorylation and nuclear translocation of NF-κB. In conclusion, PTE attenuated astrocyte-mediated inflammation and oxidative injury following IR via NF-κB inhibition. Overall, PTE is a promising neuroprotective agent.

6.
J Periodontol ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670835

RESUMO

BACKGROUND: This systematic review endeavored to investigate the effect of soft tissue phenotype modification therapy (PhMT-s) at sites with a tooth or an implant supported fixed dental prosthesis. METHODS: A comprehensive literature search was conducted by two independent examiners to identify relevant studies reporting differences in clinical, esthetic, or radiographic outcomes of interest between sites underwent PhMT-s and sites that remained untreated. Risk of bias assessment was calculated for all included studies. Meta-analyses involving endpoints of interest were performed when feasible. RESULTS: No controlled studies pertaining to tooth sites were identified. A total of six articles reporting on the outcomes of buccal soft tissue phenotype modification around implants were selected, of which, five were included in the meta-analyses. Quantitative analyses showed a weighted mean difference (WMD) of 0.98 mm (95% CI = 0.25 to 1.72 mm, P = 0.009) for change of tissue thickness; a WMD of -4.87% (95% CI = -34.27 to 24.53%, P = 0.75) for bleeding on probing (BOP); a WMD of 0.36 mm (95% CI = 0.12 to 0.59 mm, P = 0.003) for mucosal recession (MR); a WMD of 0.13 mm (95% CI = -0.11 to 0.36 mm, P = 0.30 for probing depth (PD); a WMD of 1.08 (95% CI = -0.39 to 2.55, P = 0.15) for pink esthetic score (PES), and a WMD of 0.40 mm (95% CI = -0.34 to 1.14 mm, P = 0.28) for marginal bone loss (MBL). CONCLUSIONS: Surgical modification of peri-implant soft tissue phenotype via PhMT-s may decrease the amount of MR. Future clinical trials are needed to warrant the clinical benefits of modifying soft tissue phenotype around tooth-supported restorations.

7.
J Chromatogr A ; : 460725, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31767260

RESUMO

In this work, a new analytical method based on polydopamine functionalized magnetic graphene (PDA@MG) adsorbent material has been developed to determine three triazole fungicides in water samples. As previous step, a novel polydopamine functionalized PDA@MG adsorbent material has been successfully prepared, which was characterized by fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). Based on this novel material, a new magnetic solid phase extraction (MSPE) method coupled with high performance liquid chromatography (HPLC) has been established for the determination of triazole fungicides in water samples. The main factors which could affect the experimental results were optimized. Under the optimal conditions, good linarites has been achieved in the range of 0.2-50 µg L-1, with the correlation coefficients (R2) were between 0.9962 and 0.9996. The limits of detections (LODs) were 0.0048-0.0084 µg L-1, and the relative standard deviations (RSDs) were between 1.7% and 4.8%. In addition, enrichment factors (EFs) were 572-916 times, which showed triazole fungicides residues could be accurately extracted and analyzed in this way. In the final experiment, the established method was applied to the detection of target analyzes in water samples. Satisfied results could be obtained for tebuconazole, propiconazole, and flusilazole. The recoveries of five water samples were between 69.4% and 106.4%, and the RSD were between 1.0% and 6.5%. The development method is more easy, effective, green and environmental-friendly, and has potential for application.

8.
Environ Sci Technol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31689090

RESUMO

Rejection of endocrine-disrupting compounds (EDCs) by thin-film composite (TFC) polyamide membranes remains a challenging issue in wastewater reclamation applications because of the unfavorable hydrophobic interaction between EDCs and membranes. Herein, we investigated the incorporation of hydrophilic metal organic frameworks (MOFs) into the polyamide layer to create water/EDC selective nanochannels for enhancing EDC rejection. Using MIL-101(Cr) MOF as a nanofiller, the water flux of the MOF0.20 TFC membrane (0.20 wt/v % MOF in n-hexane) was 2.3 times that of the control. The rejection rates against EDCs involving methylparaben, propylparaben, benzylparaben, and bisphenol A (BPA) by MOF0.20 were also significantly higher than the respective values of the control membrane, with the water/EDC selectivity (e.g., A/BBPA) of MOF0.20 approximately doubled compared to that of the control. Further single salt rejection and gold nanoparticle filtration tests confirmed that the hydrophilic nanochannels created by MOFs played a critical role in membrane transport, accounting for the significant enhancement of EDC rejection of the modified TFC membrane. This study demonstrates a promising membrane modification protocol using hydrophilic MOFs for achieving selective removal of EDCs and high-efficient wastewater reclamation using TFC membranes.

9.
Pathol Oncol Res ; 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31612378

RESUMO

Norcantharidin is a cantharidin demethylated analog with antitumor effects in many tumors, including cholangiocarcinoma. Autophagy suppression is known to increase chemosensitivity in cholangiocarcinoma. This study aimed to determine whether autophagy suppression accelerates apoptosis induced by norcantharidin in human cholangiocarcinoma cells. The human cholangiocarcinoma cell line QBC939 was incubated in RPMI 1640 medium with or without norcantharidin. Autophagy was induced using HBSS media with Ca2+ and Mg2+ supported by 10 mM HEPES or suppressed by treatment with 3-MA or transfection with siRNA against Atg5. The comparison was drawn between these conditions in mitochondrial membrane potential disturbance, the levels of reactive oxygen species (ROS), apoptotic proteins, and apoptosis. Cholangiocarcinoma cell apoptosis was accelerated by norcantharidin. Autophagy suppression up-regulated norcantharidin's pro-apoptotic effect, but autophagy induction weakened it. As apoptosis was accelerated, ROS production was up-regulated. Bax protein expression, cytochrome c levels and localization, mitochondrial membrane disturbance, and the levels of caspase-9, caspase-3, and cleaved PARP were higher when autophagy was suppressed, and all of those were down-regulated when autophagy was induced. To sum up, it was found that norcantharidin induced cholangiocarcinoma cell death, and autophagy suppression enhanced the pro-apoptotic action of norcantharidin, which appears to involve the mitochondrial apoptosis pathway activation and ROS generation.

10.
Environ Toxicol Pharmacol ; 73: 103271, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31627035

RESUMO

Tributyltin (TBT), a proven endocrine disrupter, was widely used in industry and agriculture. Previous research showed that TBT could alter the balance between osteogenesis and adipogenesis, which may have significant consequences for bone health. Herein, we exposed male rats to TBT chloride (TBTCl) to evaluate the deleterious effects of TBT on bone. Exposure to 50 µg kg-1 TBT resulted in a significant decrease in bone mineral density (BMD) at the femur diaphysis region in the rat. A dose-dependent increase in lipid accumulation and adipocyte number was observed in the bone marrow (BM) of the femur. Meanwhile, TBTCl treatment significantly enhanced the expression of PPARγ and attenuated the expression of Runx2 and ß-catenin in BM. In addition, serum ALP activity of TBT-exposed rats also showed a dose-dependent decrease. These results suggest that TBT could reduce BMD via inhibition of the Wnt/ß-catenin pathway and skew the adipo-osteogenic balance in the BM of rats.

11.
Phys Rev E ; 100(2-1): 023110, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31574777

RESUMO

The laminar-turbulent transition induced by two-dimensional steplike roughness is investigated focusing on the pattern of breakdown. The statistics of the turbulent burst rate is found to be significantly different from the prediction of the classical theory. A systematic investigation of the pattern of breakdown is motivated by this phenomenon. It is identified with heuristic analysis that a pattern of distributed breakdown is responsible for the deviation, in contrast to the concentrated breakdown hypothesis in the classical theory. The pattern indicates that the steps probably induced a bypass transition in present experimental setup, which is different from the current understanding about the step-induced transition. Cellular-automaton simulations are carried out to validate the heuristic analysis. The influences of quasiconcentration and non-Poisson process in spot generation on the breakdown statistics are also discussed based on the simulation results.

12.
Org Lett ; 21(22): 9133-9137, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31663763

RESUMO

A light-induced intramolecular iodine-atom transfer radical addition was reported toward iodine-substituted fluorene derivatives. A thioxanthone derivative was employed as a visible light sensitizer for this 5-exo-dig exclusive radical addition, and the newly formed vinyl iodide compounds were further proved to be effective partners for several cross-coupling reactions.

13.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(8): 689-694, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31638565

RESUMO

Objective To study the effect of deletion of Toll-like receptor 2 (TLR2) gene on insulin resistance and polarization of macrophages in mice. Methods The wild-type (WT) and TLR2 knockout (TLR2-/-) C57BL/6 male mice, aged 28 days, were selected, with 12 mice in each group. The genotype of each mouse was identified by PCR. After mice were fed with basic diet for 3 months, the glucose tolerance test (GTT) and insulin resistance test (ITT) were performed. The mononuclear cells isolated from peripheral blood were stimulated with GM-CSF/IFN-γ and M-CSF/IL-4/IL-13, respectively, to induce differentiation to M1-like and M2-like macrophages. The CD11b, F4/80, CD11c, CD206 and early growth response 2 (EGR2) were detected by flow cytometry to determine the phenotype of macrophages. The levels of TNF-α, IL-6 and IL-10 in the culture supernatant of macrophages were detected using ELISA. Results The result of PCR identification was consistent with the genotype of mice. Compared to WT mice, TLR2-/- mice exhibited the significantly improved glycemic control at 30 min during GTT and the significantly increased insulin sensitivity at 15 minutes during ITT. The flow cytometry showed that M1 markers decreased and M2 macrophages increased in the TLR2-/- mice. ELISA showed that the levels of IL-6 and TNF-α significantly decreased in the culture supernatant of M1 macrophages, while the level of IL-10 significantly increased in the culture supernatant of M2 macrophages in TLR2-/- mice compared with WT mice. Conclusion TLR2 signal has an effect on the polarization of macrophages and makes macrophages tend to switch to M1 phenotype. A higher number of pro-inflammatory factors secreted by M1 macrophages contribute to a low-grade inflammation state in the body, which leads to a decrease in glucose tolerance and insulin sensitivity.


Assuntos
Resistência à Insulina , Macrófagos , Receptor 2 Toll-Like , Animais , Resistência à Insulina/genética , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptor 2 Toll-Like/genética
14.
Chem Commun (Camb) ; 55(91): 13661-13664, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31603447

RESUMO

We report a handy, simple and inexpensive paper device for extremely sensitive detection of peroxide-based explosives. The sensing device fabricated using a curcumin derivative was capable of ultrafast sensing of triacetone triperoxide. The detection time was below 5 s. Moreover, the sensor retained full function under storage at ambient temperature for at least 120 days.

15.
Sci Rep ; 9(1): 13160, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31511592

RESUMO

Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UTI89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the dissociation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance.

16.
Sci Rep ; 9(1): 13046, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506455

RESUMO

The fabella is a sesamoid bone of the knee that can degenerate in some patients with osteoarthritis. The purpose of this study was to examine the prevalence and degeneration grades of fabellae in the Chinese population and to analyse their relationships with subject ages and knee osteoarthritis grades. The anteroposterior and lateral knee roentgenograms of 1150 subjects were recruited from the institutional database. The Kellgren-Lawrence scoring system was used to evaluate knee osteoarthritis. The degeneration grades of fabellae were scored in lateral roentgenograms by screening their shapes, sizes, subchondral sclerosis and osteophyte formation. The prevalence and degeneration of fabellae among ages, genders and knee sides were analysed by the Pearson Chi-Square test, and their relationships with knee osteoarthritis were analysed by the Spearman nonparametric correlation test. The overall prevalence of fabellae was 48.6% in 1359 knees. There was no significant difference in fabellar prevalence between the two sides (χ² = 0.025, P = 0.87437) and genders (χ² = 3.647, P = 0.05617), while the prevalence increased with the increasing ages of the subjects (χ² = 213.868, P < 0.001). The fabellar degeneration grades were correlated with age (r = 0.5288, P < 0.001) and knee osteoarthritis scores (r = 0.6892, P < 0.001). These results suggested that the fabellar prevalence and degeneration grades were correlated with age and knee osteoarthritis scores.

17.
Clin Lung Cancer ; 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31474376

RESUMO

PURPOSE: To develop a prediction model based on 18F-fludeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT) for solid pulmonary nodules (SPNs) with high malignant probability. PATIENTS AND METHODS: We retrospectively reviewed the records of CT-undetermined SPNs, which were further evaluated by PET/CT between January 2008 and December 2015. A total of 312 cases were included as a training set and 159 as a validation set. Logistic regression was applied to determine independent predictors, and a mathematical model was deduced. The area under the receiver operating characteristic curve (AUC) was compared to other models. Model fitness was assessed based on the American College of Chest Physicians guidelines. RESULTS: There were 215 (68.9%) and 127 (79.9%) malignant lesions in the training and validation sets, respectively. Eight independent predictors were identified: age [odds ratio (OR) = 1.030], male gender (OR = 0.268), smoking history (OR = 2.719), lesion diameter (OR = 1.067), spiculation (OR = 2.530), lobulation (OR = 2.614), cavity (OR = 2.847), and standardized maximum uptake value of SPNs (OR = 1.229). Our AUCs (training set, 0.858; validation set, 0.809) was better than those of previous models (Mayo: 0.685, P = .0061; Peking University People's Hospital: 0.646, P = .0180; Herder: 0.708, P = .0203; Zhejiang University: 0.757, P = .0699). The C index of the nomogram was 0.858. Our model reduced the diagnosis of indeterminate nodules (26.4% vs. 79.2%, 53.5%, 39.6%, and 34.0%, respectively) while improved sensitivity (81.3% vs. 16.4%, 49.2%, 62.5%, and 68.0%, respectively) and accuracy (65.4% vs. 16.4%, 39.6%, 52.8%, and 58.5%, respectively). CONCLUSION: Our model could permit accurate diagnoses and may be recommended to identify malignant SPNs with high malignant probability, as our data pertain to a very high-prevalence cohort only.

18.
J Int Acad Periodontol ; 21(2): 82-96, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31522155

RESUMO

AIM: Sup-epithelial connective tissue graft (SCTG) and de-epithelialized gingival graft (DGG) approaches have been investigated with a focus on post-operative morbidity but not from a clinical outcome standpoint. The aim of this systematic review was to systematically investigate the literature for coronally advanced flaps (CAFs) combined with SCTG or DGG. MATERIALS AND METHODS: Electronic and hand searches were performed to identified randomized controlled trials (RCTs) investigating the treatment of gingival recession (GRs) using CAF, with at least a 1-year of follow-up. The primary outcome was the mean root coverage (mRC), while the secondary outcomes included recession reduction (Rec Red), keratinized tissue (KT) gain, probing depth (PD) change, and clinical attachment level (CAL) gain. RESULTS: Ten RCTs with a total of 408 gingival recessions were included. The meta-analysis demonstrated that CAF + DGG is associated with superior mRC, Rec Red, KT gain, PD reduction and CAL gain. The mRC for SCTG and DGG at 1-year was 89.3% and 94.0% respectively, while the mean difference of the other clinical parameters between the two approaches was within 1 mm in favor of the DGG group. CONCLUSIONS: Limited evidence is available when comparing the two techniques, however the usage of DGG may be considered as the preferred technique of choice for autologous CTG harvesting when incorporated with a CAF.

19.
Int Dent J ; 69 Suppl 2: 7-11, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31478572

RESUMO

In implant dentistry, plaque control and oral hygiene practices are essential to limit the risk of complication and failure in the long term. All conditions around an implant in function that influence the load and pathogenicity of the microbiota are considered local risk indicators. They concern the prosthetic suprastructure design and the possibility for the patient to easily access each implant for plaque removal. Use of cemented prostheses should be limited to avoid excess cement acting as a foreign body and leading to peri-implant disease. The crown margins should be supramucosal, and the connection should be precise to avoid a gap between the implant and the suprastructure. Every implant system is characterised by a specific design, surface texture and connection type. These features may influence peri-implantitis development and progression, and the clinician should consider the risk of infection when selecting an implant. The soft-tissue conditions around the implant, the width of keratinised mucosa, and the phenotype and thickness of the mucosa are also considered major risk indicators, as the presence of any mucosal defect around an implant can increase plaque accumulation and result in tissue inflammation. The pathogenicity of the microbiota around an implant is primarily dependent on pocket depth. Deep pockets around implants should be avoided and, if present, closely monitored and/or reduced. Proximity to natural teeth presenting endodontic and/or periodontal lesions may result in implant contamination, but the influence of the type of edentulism on perio-pathogen presence is still unclear. These local conditions around an implant have a clear influence on peri-implant diseases development and progression, but there is still only limited evidence regarding their role as true risk indicators.


Assuntos
Implantes Dentários , Peri-Implantite , Consenso , Índice de Placa Dentária , Humanos , Higiene Bucal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA