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1.
Insect Sci ; 27(1): 170-184, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29938899

RESUMO

Elevated concentrations of atmospheric CO2 can alter plant secondary metabolites, which play important roles in the interactions among plants, herbivorous insects and natural enemies. However, few studies have examined the cascading effects of host plant secondary metabolites on tri-trophic interactions under elevated CO2 (eCO2 ). In this study, we determined the effects of eCO2 on the growth and foliar phenolics of Medicago truncatula and the cascading effects on two color genotypes of Acyrthosiphon pisum (pink vs. green) and their parasitoid Aphidius avenae in the field open-top chambers. Our results showed that eCO2 increased photosynthetic rate, nodule number, yield and the total phenolic content of M. truncatula. eCO2 had contrasting effects on two genotypes of A. pisum; the green genotype demonstrated increased population abundance, fecundity, growth and feeding efficiency, while the pink genotype showed decreased fitness and these were closely associated with the foliar genstein content. Furthermore, eCO2 decreased the parasitic rate of A. avenae independent of aphid genotypes. eCO2 prolonged the emergence time and reduced the emergence rate and percentage of females when associated with the green genotype, but little difference, except for increased percentage of females, was observed in A. avenae under eCO2 when associated with the pink genotype, indicating that parasitoids can perceive and discriminate the qualities of aphid hosts. We concluded that eCO2 altered plant phenolics and thus the performance of aphids and parasitoids. Our results indicate that plant phenolics vary by different abiotic and biotic stimuli and could potentially deliver the cascading effects of eCO2 to the higher trophic levels. Our results also suggest that the green genotype is expected to perform better in future eCO2 because of decreased plant resistance after its infestation and decreased parasitic rate.


Assuntos
Afídeos/fisiologia , Afídeos/parasitologia , Dióxido de Carbono/metabolismo , Medicago truncatula/química , Fenóis/metabolismo , Vespas/fisiologia , Animais , Afídeos/crescimento & desenvolvimento , Herbivoria , Interações Hospedeiro-Parasita , Larva/crescimento & desenvolvimento , Larva/fisiologia , Ninfa/crescimento & desenvolvimento , Ninfa/parasitologia , Ninfa/fisiologia , Vespas/crescimento & desenvolvimento
2.
Cancer Manag Res ; 11: 9409-9420, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31807070

RESUMO

Purpose: Red blood cell distribution width (RDW), neutrophil-to-lymphocyte ratio (NLR), and platelet count (PLT) have been reported to be associated with the prognosis of malignancies; this study aimed to evaluate the prognostic significance of the inflammatory prognostic scoring index (IPSI), comprised of RDW, N LR, and PLT for overall survival (OS) in newly diagnosed multiple myeloma patients in the bortezomib-based chemotherapy era. Patients and methods: The prognostic significance of variables associated with the OS of 175 newly diagnosed multiple myeloma patients was evaluated through univariate and multivariate analyses. The cut-off values of RDW, NLR, and PLT were obtained from references. Patients with high RDW (RDW>14) were given a score of 1; patients with high NLR (NLR>2) or low PLT (PLT≤150) were given a score of 2. According to the obtained scores, the inflammatory prognostic scoring index (IPSI) was formed, in which patients were grouped into high-risk group (4-5 points), intermediate-risk group (3 points) and low-risk group (0-2 points). Results: OS varied significantly in different IPSI groups (P< 0.001). On multivariate analysis, the IPSI was an independent prognostic factor for OS (intermediate-risk group HR 2.89, 95% CI 1.60-5.22, high risk-group HR 14.50, 95% CI 7.26-28.93, P<0.001). Importantly, with IPSI as supplement to the International Staging System (ISS), a significant difference in OS was observed among IPSI subgroups (ISS I, P<0.001; ISS II, P=0.008; ISS III, P<0.001). Conclusion: The IPSI, comprised of RDW, NLR, and PLT, played specific role in the prognosis of patients preliminarily diagnosed with multiple myeloma in the bortezomib-based chemotherapy era and could be a beneficial supplement for ISS staging.

3.
PeerJ ; 7: e7568, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31523515

RESUMO

Background & Aims: Aging is one of the risk factors of non-alcoholic fatty liver disease (NAFLD). Yet, the mechanism underlying the aging-associated NAFLD-like syndrome is not fully understood. Nicotinamide adenine dinucleotide (NAD), a ubiquitous coenzyme, has protective effects against aging. Here, we investigated the actions of NAD precursors nicotinamide riboside (NR) on the development of aging-induced NAFLD. Methods: NR supplemented food (2.5 g/kg food) was applied to aged mice for three months while normal chow to the other groups. Body weight, food intake, liver weight and fat pat mass were measured. The serum concentrations of lipid content, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and NAD were determined by biochemical assays. Pathological assessment and immunohistochemistry analysis of hepatic tissues were used to evaluate the effect of NR on NAFLD development and inflammatory infiltration. Results: NR repletion significantly reduced fat pat mass in aged mice, while not altered the body weight, food intake, and liver weight. NR repletion significantly rescued the NAD reduction in aged mice. The total cholesterol and triglyceride levels could be lowered by NR repletion in aged mice. The AST level was also significantly reduced by NR repletion in aged group, while the ALT level lowered but without significance. Notably, moderate NAFLD phenotypes, including steatosis and hepatic fibrosis could be markedly corrected by NR repletion. In addition, Kupffer cells accumulated and inflammatory infiltration could also be remarkably reversed by NR repletion in aged mice. Conclusion: Aging was associated with NAFLD-like phenotypes in mice, which could be reversed by oral NR repletion. Therefore, oral NR uptake might be a promising strategy to halt the progression of NAFLD.

4.
Hematol Oncol ; 37(4): 474-482, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31325181

RESUMO

LncRNAs play critical roles in various pathophysiological and biological processes, such as protein translation, RNA splicing, and epigenetic modification. Indeed, abundant evidences demonstrated that lncRNA act as competing endogenous RNAs (ceRNAs) to participate in tumorigenesis. However, little is known about the underlying function of lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1) in pediatric and adolescent acute myeloid leukemia (AML). The expression of LINP1 was examined in AML patient samples by qRT-PCR. Cell proliferation was examined by CCK-8 and Edu assays. ß-Galactosidase senescence assay, mGlucose uptake assay, lactate production assay, and Gene Ontology (GO) analysis were performed for functional analysis. We found that LINP1 was significantly overexpressed in AML patients at diagnosis, whereas downregulated after complete remission (CR). Furthermore, knockdown of LINP1 expression remarkably suppressed glucose uptake and AML cell maintenance. Mechanistically, LINP1 was found to inhibit the glucose metabolism by suppressing the expression of HNF4a. Both LINP1 and HNF4a knockdown reduced the expression levels of AMPK phosphorylation and WNT5A, indicating for the first time that LINP1 strengthened the HNF4a-AMPK/WNT5A signaling pathway involved in cell glucose metabolism modulation and AML cell survival. Taken together, our results indicated that LINP1 promotes the malignant phenotype of AML cells and stimulates glucose metabolism, which can be regarded as a potential prognostic marker and therapeutic target for AML.


Assuntos
Adenilato Quinase/fisiologia , Fator 4 Nuclear de Hepatócito/fisiologia , Leucemia Mieloide Aguda/genética , RNA Longo não Codificante/fisiologia , RNA Neoplásico/fisiologia , Transdução de Sinais/fisiologia , Proteína Wnt-5a/fisiologia , Adolescente , Animais , Medula Óssea/patologia , Divisão Celular , Criança , Regulação Leucêmica da Expressão Gênica , Técnicas de Silenciamento de Genes , Ontologia Genética , Glucose/metabolismo , Fator 4 Nuclear de Hepatócito/biossíntese , Fator 4 Nuclear de Hepatócito/genética , Humanos , Leucemia Mieloide Aguda/metabolismo , Leucemia Mieloide Aguda/patologia , Masculino , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Transplante de Neoplasias , Púrpura Trombocitopênica Idiopática/metabolismo , Interferência de RNA , RNA Longo não Codificante/biossíntese , RNA Longo não Codificante/genética , RNA Neoplásico/biossíntese , RNA Neoplásico/genética , RNA Interferente Pequeno/genética , Distribuição Aleatória , Indução de Remissão , Transdução de Sinais/genética , Células THP-1
6.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 47(1): 19-26, 2018 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-30146807

RESUMO

OBJECTIVE: : To investigate the effects of cysteinyl leukotrienes receptor (CysLTR) antagonists on global cerebral ischemia/reperfusion (CI/R) injury in gerbils, and to explore its mechanism. METHODS: : Totally 40 gerbils weighting 45-65 g were randomized into sham, saline, Pranlukast and HAMI 3379 groups with 10 animals in each. The CI/R model was established in gerbils by bilateral common carotid occlusion for 10 min followed by reperfusion. After ischemia, the CysLTR antagonists Pranlukast (0.1 mg/kg) and HAMI 3379 (0.1 mg/kg) were injected intraperitoneally for 5 consecutive days in the last two groups,while the former two groups were injected with saline only (10 mL/kg). After 24 h or 14 d reperfusion, neurological deficit score was evaluated and the behavioral dysfunction was assessed, respectively. And 14 d after reperfusion, the neuron morphology of cerebral cortex was observed in brain sections stained with Cresyl violet. In addition, the Iba-1 (microgila) and GFAP (astrocyte) positive cells in cerebral cortex were observed by using immunohistochemitry method. RESULTS: : CI/R models were successfully established in 21 out of 30 gerbils with 7 in saline group, 6 in Pranlukast group, and 8 in HAMI 3379 group. Compared with saline group, Pranlukast and HAMI 3379 significantly attenuated neurological deficits, improved the behavioral function 24 h after reperfusion(all P<0.01); Pranlukast and HAMI 3379 also significantly improved the behavioral function 14 days after reperfusion(P<0.05 or P<0.01). Compared with saline group, the neurological symptom scores in Pranlukast and HAMI 3379 groups presented a trend of amelioration 14 d after reperfusion, but it was not significant(P>0.05). In addition, Pranlukast and HAMI 3379 also inhibited the neuron loss and injury, suppressed microgila and astrocyte activation 14 d after reperfusion(all P<0.01). CONCLUSIONS: : CysLTR antagonists Pranlukast and HAMI 3379 have long-term neuroprotective effect on chronic brain injury induced by global cerebral ischemia/reperfusion in gerbils.


Assuntos
Lesão Encefálica Crônica , Antagonistas de Leucotrienos , Animais , Comportamento Animal/efeitos dos fármacos , Lesão Encefálica Crônica/tratamento farmacológico , Isquemia Encefálica , Gerbillinae , Antagonistas de Leucotrienos/farmacologia , Antagonistas de Leucotrienos/uso terapêutico , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêutico , Distribuição Aleatória , Receptores de Leucotrienos/metabolismo , Traumatismo por Reperfusão/tratamento farmacológico
7.
Sci Rep ; 8(1): 9964, 2018 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-29967388

RESUMO

Elevated CO2 and O3 can affect aphid performance via altering plant nutrients, however, little is known about the role of plant secondary metabolites in this process, especially for aphids feeding behaviors. We determined the effects of elevated CO2 and O3 on the growth and phenolics of alfalfa (Medicago sativa) and feeding behaviors of the pea aphids (Acyrthosiphon pisum) and cowpea aphids (Aphis craccivora). Elevated CO2 improved plant growth, but could not completely offset the negative effects of elevated O3. Elevated O3 increased foliar genistin content at the vegetative stage, increased ferulic acid at the reproductive stage, and elevated CO2 increased those at both stages. Simultaneously elevated CO2 and O3 increased foliar ferulic acid content at the reproductive stage and increased genistin content at both stages. For pea aphids, feeding efficiency was reduced under elevated CO2 at the reproductive stage and decreased under elevated O3 at the vegetative stage. For cowpea aphids, feeding efficiency was increased under elevated CO2 at the vegetative stage and decreased under elevated O3 at both stages. Simultaneously elevated CO2 and O3 decreased both aphids feeding efficiency. We concluded that CO2 and O3 independently or interactively had different effects on two aphids feeding behaviors through altering foliar ferulic acid and genistin contents.


Assuntos
Afídeos/fisiologia , Dióxido de Carbono , Comportamento Alimentar/fisiologia , Ozônio , Folhas de Planta/metabolismo , Animais , Ácidos Cumáricos/metabolismo , Isoflavonas/metabolismo , Medicago sativa/crescimento & desenvolvimento , Medicago sativa/metabolismo , Folhas de Planta/química , Metabolismo Secundário
8.
Front Plant Sci ; 9: 764, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29946327

RESUMO

Elevated ozone (O3) can alter the phenotypes of host plants particularly in induction of leaf senescence, but few reports examine the involvement of phytohormone in O3-induced changes in host phenotypes that influence the foraging quality for insects. Here, we used an ethylene (ET) receptor mutant Nr and its wild-type to determine the function of the ET signaling pathway in O3-induced leaf senescence, and bottom-up effects on the performance of Bemisia tabaci in field open-top chambers (OTCs). Our results showed that elevated O3 reduced photosynthetic efficiency and chlorophyll content and induced leaf senescence of plant regardless of plant genotype. Leaf senescence in Nr plants was alleviated relative to wild-type under elevated O3. Further analyses of foliar quality showed that elevated O3 had little effect on phytohormone-mediated defenses, but significantly increased the concentration of amino acids in two plant genotypes. Furthermore, Nr plants had lower amino acid content relative to wild-type under elevated O3. These results provided an explanation of O3-induced increase in abundance of B. tabaci. We concluded that O3-induced senescence of plant was ET signal-dependent, and positive effects of O3-induced leaf senescence on the performance of B. tabaci largely resulted from changes of nutritional quality of host plants.

9.
Mol Med Rep ; 17(2): 3288-3296, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29257228

RESUMO

Gerbils are susceptible to dietary cholesterol and prone to hypercholesterolemia and non­alcoholic fatty liver disease. The present study aimed to explore the role of sterol regulatory element binding protein (SREBP)­2 and 3­hydroxy­3­methylglutaryl CoA reductase (HMGCR) in hypercholesterolemia susceptibility in gerbils. Male gerbils were fed the normal diet or a high­fat diet (HFD) for 2 weeks, or the HFD for 2 weeks followed with the normal diet for an additional 2 weeks. Serum lipid levels and hepatic fat deposition were measured, and mRNA and protein levels of SREBP­2 and HMGCR were evaluated by quantitative polymerase chain reaction and Western blotting. In addition, the role of SREBP­2 function in cholesterol synthesis from the gerbil primary hepatic cells was also investigated by modulation of SERBP­2 expression via the transfection of SREBP­2 overexpression and knockdown plasmids, respectively. The data demonstrated that the total cholesterol and low­density lipoprotein cholesterol levels in the gerbil serum samples were rapidly and significantly elevated in response to HFD. In addition, the effect of the HFD was rapidly attenuated in the gerbils following a return to the normal diet. HMGCR expression and activation were not altered by dietary cholesterol consumption in the livers from the gerbils in model or recovery groups. HMGCR expression and activation were effectively regulated in cultured hepatic cells from the gerbils. These results indicated that the activation of SREBP­2 to HMGCR was not terminated in gerbil livers during cholesterol intake. Therefore, stable SREBP­2 expression contributes to the susceptibility of gerbils to hypercholesterolemia.


Assuntos
Expressão Gênica , Gerbillinae/genética , Hipercolesterolemia/veterinária , Proteína de Ligação a Elemento Regulador de Esterol 2/genética , Animais , Colesterol/sangue , Colesterol/metabolismo , Dieta Hiperlipídica/efeitos adversos , Regulação da Expressão Gênica , Gerbillinae/sangue , Gerbillinae/metabolismo , Hidroximetilglutaril-CoA Redutases/genética , Hidroximetilglutaril-CoA Redutases/metabolismo , Hipercolesterolemia/sangue , Hipercolesterolemia/etiologia , Hipercolesterolemia/genética , Metabolismo dos Lipídeos , Lipídeos/sangue , Fígado/metabolismo , Fígado/patologia , Masculino , Proteína de Ligação a Elemento Regulador de Esterol 2/metabolismo
10.
Animal Model Exp Med ; 1(1): 62-67, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30891548

RESUMO

Background: In the previous study, we established an ischemia-prone gerbil population (IG), which was selectively bred to increase the incidence of unilateral carotid arterial occlusion (UCO)-induced ischemia in Mongolian gerbils. However, if the characteristics of ischemia model in IG are the same as those in general gerbils (GG), and if the neurological symptoms are associated with the neurological insults in IG is still unclear. Methods: In the present study, we evaluated the UCO model in IG by analyzing neurological symptoms, neurological injury in the hippocampal CA1 region and compared with GG. Results: The data showed that the ratios of neurological symptom scores ≥ 2 in the IG and GG groups were 65.0% vs 30.0%, respectively, and were significantly different (P < .01).The neuronal damage following a UCO ischemic insult in the IG group was more severe compared to the GG group. There was a high correlation between the neurological insults' scale and the neurological symptom score in the IG and GG groups (r = .979 and .943 in the IG and GG groups, respectively). In animals with mild neurological symptom scores (2 and 3), the neuronal insults were significantly different between female and male gerbils in both IG and GG. Conclusion: Our findings suggest that IG population would likely be more advantageous to establish an ischemic model.

11.
Sci China Life Sci ; 60(8): 816-825, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28785951

RESUMO

Climatic variations are becoming important limiting factors for agriculture productivity, as they not only directly affect the plant net primary productivity but can also modulate the outbreak of plant diseases and pests. Elevated CO2 and O3 are two important climatic factors that have been widely studied before. Elevated CO2 or O3 alters the host plant physiology and affects the vector insects and plant viruses via bottom-up effects of the host plants. Many studies have shown that elevated CO2 or O3 decreases the plant nitrogen content, which modulates the characteristics of vector insects. Recent evidence also reveals that hormone-dependent signaling pathways play a critical role in regulating the response of insects and plant viruses to elevated CO2 or O3. In the current review, we describe how elevated CO2 or O3 affects the vector insects and plant viruses by altering the SA and JA signaling pathways. We also discuss how changes in the feeding behavior of vector insects or the occurrence of plant viruses affects the interactions between vector insects and plant viruses under elevated CO2 or O3. We suggest that new insights into the upstream network that regulates hormone signaling and top-down effects of natural enemies would provide a comprehensive understanding of the complex interactions taking place under elevated CO2 or O3.


Assuntos
Dióxido de Carbono/metabolismo , Insetos Vetores/fisiologia , Ozônio/metabolismo , Reguladores de Crescimento de Planta/metabolismo , Vírus de Plantas/fisiologia , Plantas/virologia , Animais , Ciclopentanos/metabolismo , Interações Hospedeiro-Patógeno , Insetos/virologia , Oxilipinas/metabolismo , Doenças das Plantas/virologia , Ácido Salicílico/metabolismo , Transdução de Sinais
12.
Exp Ther Med ; 13(5): 1725-1734, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28565759

RESUMO

Ampelopsin (AMP) is isolated from the Chinese medicinal herb Ampelopsis grossedentata (Hand-Mazz) and has been associated with numerous biological and pharmacological activities. However, it is not clear whether AMP has a direct protective effect on cerebral ischemia reperfusion injury. Therefore, the present study investigated its role in acute brain injury following focal cerebral ischemia in rats. The current study induced transient focal cerebral ischemia by performing middle cerebral artery occlusion (MCAO) for 60 min, followed by 24 h of reperfusion. Rats were exposed to 40, 80 and 160 mg/kg AMP by oral administration 30 min prior to MCAO and the cysteinyl leukotriene receptor 1-antagonist, pranlukast (0.1 mg/kg, i.p.) was used as a positive control. Neurological deficit scores were observed and an inclined board test was used to assess behavioral dysfunction. The coronal slices were stained with 3,5-triphenyltetrazolium chloride to determine the infarct volume and brain edema. Neuronal morphology was assessed in brain sections stained with cresyl violet and degenerating neurons were identified using Fluoro-Jade B staining. Blood-brain barrier permeability was determined with immunoglobulin (Ig)G immunohistochemistry. Interleukin (IL)-1ß, tumor necrosis factor-α (TNF-α) in serum and cerebrospinal fluid were measured using ELISA kits. AMP at 80 and 160 mg/kg attenuated neurological deficits, reduced infarct volume, brain edema, IgG exudation and neuron degeneration and loss. Similar to pranlukast, AMP also inhibited the MCAO-induced IL-1ß and TNF-α release. Thus, AMP has a neuroprotective effect on acute brain injury following focal cerebral ischemia in rats at an effective oral dose of 80-160 mg/kg. The results of the current study indicate a therapeutic role for AMP in the treatment of ischemic stroke.

13.
Medicine (Baltimore) ; 96(48): e8783, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29310353

RESUMO

RATIONALE: Complex pelvic ring fracture associated with floating knee is comparatively rare which usually results from high-energy trauma including vehicle-related accidence, falls from height, and earthquake-related injury. To our knowledge, few literatures have documented such injuries in the individual patient. Management of both injuries present challenges for surgical management and postoperative care. The purpose of this study is to prove the feasibility and benefits of damage control orthopedics (DCO). PATIENT CONCERN: Our case involved a 45-year-old lady who was hit by a dilapidated building. The patient was anxious, pale and hemodynamically stable at the initial examination. The pelvis was unstable and there were obvious deformities in the left lower extremities. Significant degloved injuries in the left leg were noted. Her radiographs and physical examination verified the above signs. DIAGNOSES: Unstable pelvic fractures, multiple fractures of bilateral lower limbs with floating knee injury, multiple pelvic and rib fractures and multiple degloving injuries and soft tissue contusion formed the characteristics of the multiple-injury. INTERVENTIONS: The algorithm of DCO was determined as the treatment. Early simplified procedures such as wound debridement, pelvis fixation, closed reduction and EF of the right shoulder joint, and chest wall fixation were conducted as soon as possible. After a period of time, internal fixations were applied to the fracture sites. The subsequent functional exercise was also conducted in accordance with this algorithm. OUTCOMES: This patient got recovery after the treatments which were guided by the criterion of DCO. The restoration of limb functional and the quality of life greatly improved. LESSONS: The DCO plays a decisive role in the first aid and follow-up treatment of this patient. The guidelines of management of complex pelvic ring injuries and floating knee should be established by authorities.


Assuntos
Fraturas Ósseas/terapia , Traumatismos do Joelho/terapia , Traumatismo Múltiplo/terapia , Ossos Pélvicos/lesões , Contusões/etiologia , Contusões/terapia , Desbridamento , Feminino , Primeiros Socorros , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/etiologia , Humanos , Traumatismos do Joelho/diagnóstico por imagem , Traumatismos do Joelho/etiologia , Traumatismos da Perna/diagnóstico por imagem , Traumatismos da Perna/etiologia , Traumatismos da Perna/terapia , Pessoa de Meia-Idade , Traumatismo Múltiplo/diagnóstico por imagem , Traumatismo Múltiplo/etiologia , Ossos Pélvicos/diagnóstico por imagem , Fraturas das Costelas/diagnóstico por imagem , Fraturas das Costelas/etiologia , Fraturas das Costelas/terapia
14.
Dose Response ; 14(4): 1559325816678492, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27928219

RESUMO

Salidroside (SDS) has been reported to have anti-inflammatory properties. The objective of this study was to investigate the protective effect of SDS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. BALB/c mice were pretreated with SDS 1 hour before intranasal instillation of LPS. Seven hours after LPS administration, the myeloperoxidase in histology of lungs, lung wet/dry ratio, and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of pro-inflammatory cytokines, tumor necrosis factor α (TNF-α), interleukin-1ß (IL 1ß), and IL-6 in the BALF were measured by enzyme-linked immunosorbent assay. The expression of Toll-like receptor 4 (TLR4), inhibitor of nuclear factor-kappa B (IκB-α), and nuclear factor-kappa B (NF-κB) p65 was detected by Western blot. The SDS reduced the inflammatory cells in BALF, decreased the wet/dry ratio of lungs, attenuated the LPS-induced histological alterations in the lung, and inhibited the production of TNF-α, IL-1ß, and IL-6. Western blot showed that SDS efficiently inhibited the phosphorylation of IκB-α, p65 NF-κB, and the expression of TLR4. These data show that the anti-inflammatory effects of SDS (at least 20 mg/kg) against LPS-induced ALI due to its ability to inhibit TLR4 mediated the NF-κB signaling pathways. The SDS may represent a novel strategy for treating LPS-induced ALI.

15.
Front Plant Sci ; 7: 1680, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27881989

RESUMO

Elevated atmospheric CO2 typically enhances photosynthesis of C3 plants and alters primary and secondary metabolites in plant tissue. By modifying the defensive signaling pathways in host plants, elevated CO2 could potentially affect the interactions between plants, viruses, and insects that vector viruses. R gene-mediated resistance in plants represents an efficient and highly specific defense against pathogens and herbivorous insects. The current study determined the effect of elevated CO2 on tomato plants with and without the nematode resistance gene Mi-1.2, which also confers resistance to some sap-sucking insects including whitefly, Bemisia tabaci. Furthermore, the subsequent effects of elevated CO2 on the performance of the vector whiteflies and the severity of Tomato yellow leaf curl virus (TYLCV) were also determined. The results showed that elevated CO2 increased the biomass, plant height, and photosynthetic rate of both the Moneymaker and the Mi-1.2 genotype. Elevated CO2 decreased TYLCV disease incidence and severity for Moneymaker plants but had the opposite effect on Mi-1.2 plants whether the plants were agroinoculated or inoculated via B. tabaci feeding. Elevated CO2 increased the salicylic acid (SA)-dependent signaling pathway on Moneymaker plants but decreased the SA-signaling pathway on Mi-1.2 plants when infected by TYLCV. Elevated CO2 did not significantly affect B. tabaci fitness or the ability of viruliferous B. tabaci to transmit virus regardless of plant genotype. The results indicate that elevated CO2 increases the resistance of Moneymaker plants but decreases the resistance of Mi-1.2 plants against TYLCV, whether the plants are agroinoculated or inoculated by the vector. Our results suggest that plant genotypes containing the R gene Mi-1.2 will be more vulnerable to TYLCV and perhaps to other plant viruses under elevated CO2 conditions.

16.
BMC Oral Health ; 16(1): 112, 2016 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-27793138

RESUMO

BACKGROUND: Malocclusion is a common disease of oral and maxillofacial region. The study was aimed to investigate levels changes of periodontal pathogens in malocclusion patients before, during and after orthodontic treatments, and to confirm the difference between adults and children. METHOD: One hundred and eight malocclusion patients (46 adults and 62 children at the school-age) were randomly selected and received orthodontic treatment with fixed orthodontic appliances. Subgingival plaques were Porphyromonas gingivalis (P.gingivalis), Fusobacterium nucleatum (F. nucleatum), Prevotella intermedia (P. intermedia) and Tannerella forsythensis (T. forsythensis) collected from the observed regions before and after treatment. Clinical indexes, including plaque index (PLI), gingival index (GI), sulcus bleeding index (SBI), probing depth (PD) and attachment loss (AL) of observed teeth were examined. RESULTS: The detection rates of P.gingivalis, F. nucleatum, P. intermedia and T. forsythensis increased from baseline to the third month without significant difference, and then returned to pretreatment levels 12 month after applying fixed orthodontic appliances. Adults' percentage contents of P.gingivalis, F. nucleatum, P. intermedia and T. forsythensis were significantly higher than those of children at baseline and the first month, but not obvious at the third month. PLI and SBI were increased from baseline to the first and to the third month both in adults and children groups. Besides, PD were increased from baseline to first month, followed by a downward trend in the third month; however, all patients were failed to detect with AL. CONCLUSIONS: Periodontal and microbiological statuses of malocclusion patients may be influenced by fixed orthodontic appliances in both adults and children, more significant in children than in adults. Some microbiological indexes have synchronous trend with the clinical indexes. Long-term efficacy of fixed orthodontic appliances for malocclusion should be confirmed by future researches.


Assuntos
Índice de Placa Dentária , Placa Dentária , Má Oclusão/terapia , Aparelhos Ortodônticos , Adulto , Aggregatibacter actinomycetemcomitans , Criança , Humanos , Índice Periodontal , Porphyromonas gingivalis/isolamento & purificação , Prevotella intermedia
17.
Biochem Biophys Res Commun ; 458(2): 328-33, 2015 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-25656574

RESUMO

Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies.


Assuntos
MicroRNAs/metabolismo , Mieloma Múltiplo/metabolismo , Mieloma Múltiplo/patologia , NF-kappa B/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Transdução de Sinais , Linhagem Celular Tumoral , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/genética , Mieloma Múltiplo/genética , NF-kappa B/genética , Complexo de Endopeptidases do Proteassoma/genética
18.
Haematologica ; 99(12): 1834-45, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25193962

RESUMO

Germinal center lymphoma is a heterogeneous human lymphoma entity. Here we report that constitutive activity of SHP2 (PTPN11) and its downstream kinase ERK is essential for the viability of germinal center lymphoma cells and disease progression. Mechanistically, SHP2/ERK inhibition impedes c-Myc transcriptional activity, which results in the repression of proliferative phenotype signatures of germinal center lymphoma. Furthermore, SHP2/ERK signaling is required to maintain the CD19/c-Myc loop, which preferentially promotes survival of a distinct subtype of germinal center lymphoma cells carrying the MYC/IGH translocation. These findings demonstrate a critical function for SHP2/ERK signaling upstream of c-Myc in germinal center lymphoma cells and provide a rationale for targeting SHP2 in the therapy of germinal center lymphoma.


Assuntos
Centro Germinativo/patologia , Cadeias Pesadas de Imunoglobulinas/genética , Linfoma/patologia , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo , Proteínas Proto-Oncogênicas c-myc/genética , Translocação Genética/genética , Apoptose , Western Blotting , Ciclo Celular , Proliferação de Células , Citometria de Fluxo , Imunofluorescência , Centro Germinativo/metabolismo , Humanos , Técnicas Imunoenzimáticas , Imunoprecipitação , Linfoma/genética , Linfoma/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/genética , Proteína Quinase 3 Ativada por Mitógeno/genética , Proteína Tirosina Fosfatase não Receptora Tipo 11/antagonistas & inibidores , Proteína Tirosina Fosfatase não Receptora Tipo 11/genética , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais , Células Tumorais Cultivadas
19.
Biomed Res Int ; 2014: 410480, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25006576

RESUMO

The objective of this work was to establish a novel Mongolian gerbil (Meriones unguiculatus) hyperlipidemia model and to investigate its susceptibility genetic basis. Two rodent (gerbil and rat) hyperlipidemia models were induced by feeding a high fat/high-cholesterol (HF/HC) diet. There were significant increases of serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in gerbils within a 4-week modeling period. About 10-30% of >8-month-old individuals developed hyperlipidemia spontaneously. The apolipoprotein E (ApoE) gene was cloned by merging a sequence of rapid amplification of cDNA ends (RACE) and nested polymerase chain reaction products. The results revealed an open reading frame of 948 bp, encoding a protein of 298 amino acids. The gene without a 5'-UTR region in the first intron was highly homologous to human Apo-A-I and rat Apo-A-IV. The distribution of expression of the ApoE gene in liver, brain, heart, lung, kidney, and adrenal gland was detected by an ABC immunohistochemical procedure. Three single nucleotide polymorphisms (SNPs; C97T, G781T, and A1774T) were first found using PCR-single-strand conformation polymorphism (PCR-SSCP) in a closed population containing 444 animals. Correlation analysis confirmed that new SNPs, age, and gender were associated significantly (P < 0.05) with hyperlipidemia.


Assuntos
Apolipoproteínas E/genética , Gerbillinae/genética , Hiperlipidemias/genética , Animais , Clonagem Molecular , Modelos Animais de Doenças , Frequência do Gene/genética , Predisposição Genética para Doença , Genoma/genética , Humanos , Hiperlipidemias/sangue , Hiperlipidemias/patologia , Rim/metabolismo , Rim/patologia , Masculino , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único/genética , Polimorfismo Conformacional de Fita Simples/genética , Característica Quantitativa Herdável , Ratos Sprague-Dawley , Análise de Sequência de DNA
20.
Chin Med J (Engl) ; 125(15): 2777-80, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22931993

RESUMO

The incidence of multiple noncontiguous spinal injuries (MNSI) in the cervical spine is rare but has catastrophic consequences. The patient in this report was a 34-year-old woman with five-level cervical MNSI. CT and MRI showed that injuries included atlantoaxial instability, burst fracture of C6, dislocation of C6/7, rupture of the intervertebal disc or ligamentous complex, and irreversible cord damage. The mechanism for this case was a combined pattern of hyperflexion, compression, and hyperextension injuries. A review of the literature revealed that this case is the first report in the literature of a vehicle related accident causing five-level noncontiguous injuries of the cervical spine.


Assuntos
Vértebras Cervicais/lesões , Traumatismos da Coluna Vertebral/diagnóstico , Adulto , Vértebras Cervicais/diagnóstico por imagem , Feminino , Humanos , Imagem por Ressonância Magnética , Traumatismos da Coluna Vertebral/diagnóstico por imagem , Tomografia Computadorizada por Raios X
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