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1.
Bioresour Technol ; : 126226, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34798250

RESUMO

To improve the efficiency of methane production from chicken manure (CM) anaerobic digestion, the mechanism of coal slime (CS) as an additive on methane production characteristics were investigated. The results showed that adding an appropriate amount of CS quickened the start of the fermentation and effectively increased the methane yield. In addition, the pH changed in a stable manner in the liquid phase, and the concentrations of total ammonia nitrogen (TAN) and free ammonia nitrogen (FAN) were reduced. Moreover, organic matter was decomposed and volatile fatty acids (VFAs) were consumed effectively. The abundance of Bacteroides in the bacterial community and Methanosarcina in the archaea was increased. In addition, the reduction of CO2 was the main methanogenic pathway, and adding CS raised the abundance of genes for key enzymes in metabolic pathways during methane metabolism. The results provide a novel method for the efficient methane production from CM.

2.
Ann Palliat Med ; 10(10): 10971-10985, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34763460

RESUMO

BACKGROUND: This study aimed to explore the clinical efficacy of dydrogesterone in treating recurrent spontaneous abortion (RSA), analyze the influence of dydrogesterone on cellular immune factors, and provide evidence for clinical medication. METHODS: We used the China National Knowledge Infrastructure (CNKI) platform, Wanfang Data resource, PubMed, Web of Science, and Embase database to conduct a literature search to screen clinical studies published between 2005 and 2021 concerning dydrogesterone treatment for RSA. Stata 16.0 was used for meta-analysis and sensitivity analysis, and Begg's funnel chart was used to test publication bias. RESULTS: Only 13 studies, which included a total of 2,454 RSA patients, met the study inclusion criteria. The experimental group was treated with dydrogesterone, and the control group was treated with progesterone, human chorionic gonadotropin (hCG), placebo, or active immunization. Meta-analysis showed that the pregnancy success rate of the experimental group was higher than the control group, and the adverse reaction rate was lower than the control group. In addition, subgroup analysis also revealed that the experimental group had a higher pregnancy success rate than the control group and a lower adverse reaction rate. Levels of progesterone and hCG in the experimental group were dramatically higher than the control group after treatment. The experimental group also had higher levels of interleukin 4 (IL-4) and interleukin 10 (IL-10) than the control group, while levels of interferon-gamma (IFN-γ) were lower. DISCUSSION: Dydrogesterone, a safe and effective synthetic progesterone drug, had a significant clinical effect on RSA and effectively improved hormone levels and related cellular immune factors in RSA patients.


Assuntos
Aborto Habitual , Didrogesterona , Aborto Habitual/tratamento farmacológico , Aborto Habitual/prevenção & controle , China , Didrogesterona/uso terapêutico , Feminino , Humanos , Fatores Imunológicos , Gravidez , Progesterona
3.
Open Life Sci ; 16(1): 1022-1036, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616915

RESUMO

Mevalonate pyrophosphate decarboxylase (MPD) is a key enzyme in terpenoid biosynthesis. MPD plays an important role in the upstream regulation of secondary plant metabolism. However, studies on the MPD gene are relatively very few despite its importance in plant metabolism. Currently, no systematic analysis has been conducted on the MPD gene in plants under the order Apiales, which comprises important medicinal plants such as Panax ginseng and Panax notoginseng. This study sought to explore the structural characteristics of the MPD gene and the effect of adaptive evolution on the gene by comparing and analyzing MPD gene sequences of different campanulids species. For that, phylogenetic and adaptive evolution analyses were carried out using sequences for 11 Campanulids species. MPD sequence characteristics of each species were then analyzed, and the collinearity analysis of the genes was performed. As a result, a total of 21 MPD proteins were identified in 11 Campanulids species through BLAST analysis. Phylogenetic analysis, physical and chemical properties prediction, gene family analysis, and gene structure prediction showed that the MPD gene has undergone purifying selection and exhibited highly conserved structure. Analysis of physicochemical properties further showed that the MPD protein was a hydrophilic protein without a transmembrane region. Moreover, collinearity analysis in Apiales showed that MPD gene on chromosome 2 of D. carota and chromosome 1 of C. sativum were collinear. The findings showed that MPD gene is highly conserved. This may be a common characteristic of all essential enzymes in the biosynthesis pathways of medicinal plants. Notably, MPD gene is significantly affected by environmental factors which subsequently modulate its expression. The current study's findings provide a basis for follow-up studies on MPD gene and key enzymes in other medicinal plants.

4.
Aging (Albany NY) ; 13(20): 23831-23841, 2021 10 29.
Artigo em Inglês | MEDLINE | ID: mdl-34716285

RESUMO

BACKGROUND: Osteosarcoma (OS) is a highly prevalent bone malignancy with poor clinical outcomes. Expression of the circular RNA, hsa_circ_0078767 (circFAM120B) is elevated in OS, however, its mechanisms in OS are unclear. METHODS: CircFAM120B levels were detected in OS tissue and cell lines. Silenced circFAM120B experiments were performed to assess its effects on OS in vitro cancer phenotypes and in vivo tumor growth. Then, bioinformatics analyses were used to predict circFAM120B target microRNAs (miRNAs) and associated genes. RESULTS: CircFAM120B and the transcription factor, PTBP1 were elevated in OS tissue and cell lines, while miR-1205 was poorly expressed. Silenced circFAM120B significantly suppressed in vitro OS cell proliferation and invasion, and inhibited in vivo tumor growth. CircFAM120B also appeared to function as an miR-1205 sponge, as miR-1205 bound to PTBP1. Interestingly, overexpressed PTBP1 (or miR-1205 inhibition) reversed the inhibitory effects mediated by circFAM120B downregulation in OS cells. CONCLUSION: We hypothesize circFAM120B functions as a miR-1205 sponge to elevate PTBP1 levels, enhancing OS progression and associated malignant phenotypes. Thus, circFAM120B may function as a crucial mediator during OS progression.

5.
Soft Matter ; 17(40): 9040-9046, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34569588

RESUMO

In previous studies, the increasing alkyl length of liquid crystalline molecules enhanced the chiral transfer and resulted in better CPL performance. But no work concerned the influence of alkyl lengths on CPL properties for non-liquid crystalline systems. In this research, three R-binaphthol-based cyanostilbene derivatives with various alkyl chains (BC-5, BC-8 and BC-12) were prepared in yields of 60-69%. They did not exhibit liquid crystalline behavior but were seen as soft materials at room temperature. They displayed excellent AIE fluorescence in aggregated states. Chiroptical investigations suggested good CD and CPL properties for their cyanostilbene units, indicating the successful chiral transfer from the binaphthol moieties to cyanostilbene units. Moreover, the values of gabs for CD signals and glum for CPL signals displayed the changing order of BC-5 > BC-8 > BC-12. These results suggested that the shorter alkyl chains for non-liquid crystalline systems led to stronger CPL emission, which was opposite to the results of the liquid crystalline molecules. This work provided a new strategy for the design and synthesis of chiroptical materials with good CPL properties based on non-liquid crystalline molecules.

6.
J Fluoresc ; 31(5): 1555-1565, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34338968

RESUMO

A novel fluorescein-bridged perylene bisimide (PBI) dimer for liquid crystal (LC) with geometrically symmetric structure was developed. The mesomorphic results indicated that the energetically stable and unstable conformers of fluorescein fragments could lead to the transformation of mesophases from a hexagonal columnar mesophase to an uncertain phase at 136.9 °C in heating, whilst a stable hexagonal columnar mesophase maintained between 175.6 °C and 58.6 °C in cooling. The selectively excited fluorescence characters in THF solution demonstrated that the fluorescence resonance energy transfer (FRET) effect between fluorescein fragments and PBI unites could provide a means to effectively impose strong fluorescence of the dimeric PBIs modified with suitable chromophore at the N-imide position, which alternatively serves as a platform for the further study of multi-functional PBI-based LCs.

7.
Molecules ; 26(14)2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34299563

RESUMO

A new terthiophene-based imidazole luminophore 5,5'-(1H-thieno[3,4-d]imidazole-4,6-diyl)bis(thiophene-2-carboxylic acid) (TIBTCH2, 5) was synthesized in one step from previously reported 4,6-di(thiophen-2-yl)-1H-thieno[3,4-d]imidazole (DTTI, 4), and their photophysical properties were studied and compared accordingly. Under solvothermal conditions, reacting 5 with Mn(OAc)2 yielded a new three-dimensional metal-organic framework (MOF, 6) which was structurally defined by single-crystal X-ray diffraction. In 6, all Mn(II) ions octahedrally bind to carboxylate-O atoms to form a linear Mn3 secondary building unit (SBU) that contains three distinct coordination modes. Importantly, 6 exhibits dual functional properties of ligand-based emission and metal-based magnetic behaviors.

8.
Bioresour Technol ; 337: 125437, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34320734

RESUMO

The anaerobic degradation of coal combined with straw biomass can promote the methane production. The biogas production potential and metabolic pathway were explored via the co-digestion simulation experiment of coal and corn straw. The results showed that 2 g of corn straw combined respectively with 4 g of bituminous coal A, 6 g of bituminous coal B and 4 g of bituminous coal C resulted in highest methane yields. The structure of lignocellulose in corn straw was partially degraded into guaiacyl and syringyl units. Meanwhile, the content of biodegradable tyrosine like protein and soluble microbial by-products in liquid phase significantly decreased. Significantly, the structure of archaea altered from aceticlastic to hydrogenotrophic methanogens when the fermentation substrate changed from high to low rank coal. The proportion of hydrogenotrophic methanogens was significantly higher than that of aceticlastic and methylotrophic methanogens, and the hydrogenotrophic pathway was dominant than the aceticlastic pathway.


Assuntos
Carvão Mineral , Zea mays , Anaerobiose , Biocombustíveis , Fermentação , Metano
9.
Bioengineered ; 12(1): 3159-3176, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34187307

RESUMO

N6-methyladenosine (m6A) RNA methylation regulators play a regulatory role in tumor pathogenesis and development. However, the role of m6A regulator genes in ovarian cancer (OC) has not been fully elucidated. This study aims to investigate the mRNA expressions, clinicopathological features, and prognostic values of m6A regulators in OC. Here, we demonstrate that the 17 m6A RNA methylation regulators are differentially expressed in ovarian cancer and normal tissues. By using consensus clustering, all ovarian cancer patients can be divided into two subgroups (cluster 1 and 2) based on the expression of 17 m6A RNA methylation regulators. Using Gene Set Enrichment Analysis, we identified that cluster 1 was most connected to oxidative phosphorylation pathways. Regression models identified that prognosis is associated with HNRNPA2B1, KIAA1429, and WTAP. qRT-PCR result show that the expression trends of HNRNPA2B1 and KIAA1429 are consistent with the predicted results. Multivariate Cox regression analysis results show that the risk score was an independent predictive factor in OV. The overall survival of high-risk patients was significantly shorter than that of low-risk patients. ROC curve analysis showed that the prognostic signature precisely predicted the 5-year survival of OV patients. A nomogram was developed to predict each patient's survival probability and well calibrated and showed a satisfactory discrimination. Dendritic fraction, macrophage fraction, and neutrophil fraction showed higher fraction in high-risk patients. In conclusion, m6A RNA methylation regulators are vital participants in ovarian cancer pathology, and three-gene mRNA levels are valuable factors for prognosis predictions.

10.
Int J Mol Med ; 48(2)2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34132355

RESUMO

Gypenoside XVII (GP­17), one of the dominant active components of Gynostemma pentaphyllum, has been studied extensively and found to have a variety of pharmacological effects, including neuroprotective properties. However, the neuroprotective effects of GP­17 against spinal cord injury (SCI), as well as its underlying mechanisms of action remain unknown. The present study aimed to investigate the effects of GP­17 on motor recovery and histopathological changes following SCI and to elucidate the mechanisms underlying its neuroprotective effects in a mouse model of SCI. Motor recovery was evaluated using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. Spinal cord edema was detected by the wet/dry weight method. H&E staining was performed to examine the effect of GP­17 on spinal cord damage. Inflammatory response production was assessed by ELISA. Candidate miRNAs were identified following the integrated analysis of the Gene Expression Omnibus (GEO) dataset GSE67515. Western blot analysis was also performed to detect the expression levels of associated proteins. The results revealed that GP­17 treatment improved functional recovery, and suppressed neuronal apoptosis and the inflammatory response in the mouse model of SCI. Moreover, it was observed that miR­21 expression was downregulated following SCI, whereas it was upregulated following the administration of GP­17. The inhibition of miR­21 eliminated the protective effects of GP­17 on SCI­induced neuronal apoptosis and the inflammatory response. In addition, phosphatase and tensin homologue (PTEN), a key molecule in the activation of the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway, was identified as a target of miR­21, and PTEN expression was downregulated by GP­17 through miR­21. Furthermore, the PTEN/AKT/mTOR pathway was inactivated by SCI, whereas it was re­activated by GP­17 through the regulation of miR­21 in mice with SCI. On the whole, the findings of the present study suggest that GP­17 plays a protective role in SCI via regulating the miR­21/PTEN/AKT/mTOR pathway.

11.
Environ Sci Technol ; 55(10): 6688-6699, 2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-33902278

RESUMO

While carboxylic acids are important components in both particle and gas phases in the atmosphere, their sources and partitioning are not fully understood. In this study, we present real-time measurements of both particle- and gas-phase concentrations for five of the most common and abundant low-molecular-weight carboxylic acids (LMWCA) in a rural region in the southeastern U.S. in Fall 2016. Through comparison with secondary organic aerosol (SOA) tracers, we find that isoprene was the most important local precursor for all five LMWCA but via different pathways. We propose that monocarboxylic acids (formic and acetic acids) were mainly formed through gas-phase photochemical reactions, while dicarboxylic acids (oxalic, malonic, and succinic acids) were predominantly from aqueous processing. Unexpectedly high concentrations of particle-phase formic and acetic acids (in the form of formate and acetate, respectively) were observed and likely the components of long-range transport organic aerosol (OA), decoupled from their gas-phase counterparts. In addition, an extraordinarily strong correlation (R2 = 0.90) was observed between a particulate LMWCA and aged SOA, which we tentatively attribute to boundary layer dynamics.


Assuntos
Atmosfera , Ácidos Carboxílicos , Aerossóis , Sudeste dos Estados Unidos
12.
Spectrochim Acta A Mol Biomol Spectrosc ; 256: 119744, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33819762

RESUMO

Although some reports on sensing ClO- had been presented, the ClO- sensor with high selectivity and sensitivity in aqueous media was still expected. Herein, an effective fluorescent sensor for ClO- in aqueous media was designed and synthesized by simple procedure based on cyanostilbene derivative (TCS). TCS exhibited strong fluorescence in aqueous media, which could be selectively quenched by ClO- among various species. The detection limit was as low as 3.2 × 10-8 M. The sensing mechanism of the oxidation of sulfur in thiophene unit was confirmed by the FT-IR spectrum, fluorescence Job's plot, 1H NMR spectrum and MS spectrum. This sensor was successfully applied on detecting ClO- in real sample and living-cell imaging, suggesting its potential application for sensing ClO- in both vitro assay and vivo environment.


Assuntos
Corantes Fluorescentes , Tiofenos , Bases de Schiff , Espectroscopia de Infravermelho com Transformada de Fourier , Água
13.
Bioresour Technol ; 332: 125072, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33826981

RESUMO

In order to increase the utilization of coal slime, realize efficient utilization of resources and protect the environment, the feasibility of anaerobic fermentation technology employing coal slime was explored. The biodegradation of coal slimes and its influence on the utilization characteristics were analyzed using biogas production simulations, drying dehydration and thermogravimetric (TG) analysis. The results showed that the organic matter in various coal slimes could be converted to biomethane. In addition, the main methanogenic pathway was the reduction of CO2. Moreover, lower the metamorphic degree of coal slimes and higher the ash content, more conducive were they to the dehydration of coal slimes. After biodegradation, the temperatures of four coal slimes during the stages of release of moisture, volatile combustion, residual coke combustion and burnout advanced to varying degrees. Moreover, the combustion performance improved. The research results provided a novel idea for the efficient utilization of coal slime.


Assuntos
Biocombustíveis , Carvão Mineral , Anaerobiose , Cinza de Carvão , Fermentação , Tecnologia
14.
Sci Rep ; 11(1): 7590, 2021 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-33828156

RESUMO

Gastro-esophageal (GE) cancers are one of the major causes of cancer-related death in the world. There is a need for novel biomarkers in the management of GE cancers, to yield predictive response to the available therapies. Our study aims to identify leading genes that are differentially regulated in patients with these cancers. We explored the expression data for those genes whose protein products can be detected in the plasma using the Cancer Genome Atlas to identify leading genes that are differentially regulated in patients with GE cancers. Our work predicted several candidates as potential biomarkers for distinct stages of GE cancers, including previously identified CST1, INHBA, STMN1, whose expression correlated with cancer recurrence, or resistance to adjuvant therapies or surgery. To define the predictive accuracy of these genes as possible biomarkers, we constructed a co-expression network and performed complex network analysis to measure the importance of the genes in terms of a ratio of closeness centrality (RCC). Furthermore, to measure the significance of these differentially regulated genes, we constructed an SVM classifier using machine learning approach and verified these genes by using receiver operator characteristic (ROC) curve as an evaluation metric. The area under the curve measure was > 0.9 for both the overexpressed and downregulated genes suggesting the potential use and reliability of these candidates as biomarkers. In summary, we identified leading differentially expressed genes in GE cancers that can be detected in the plasma proteome. These genes have potential to become diagnostic and therapeutic biomarkers for early detection of cancer, recurrence following surgery and for development of targeted treatment.


Assuntos
Neoplasias Esofágicas/genética , Neoplasias Gástricas/genética , Biomarcadores Tumorais/sangue , Bases de Dados Genéticas , Detecção Precoce de Câncer/métodos , Neoplasias Esofágicas/metabolismo , Expressão Gênica/genética , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica/genética , Redes Reguladoras de Genes/genética , Humanos , MicroRNAs/genética , Recidiva Local de Neoplasia/genética , Plasma/metabolismo , Proteoma/genética , Proteômica/métodos , Curva ROC , Reprodutibilidade dos Testes , Neoplasias Gástricas/metabolismo , Máquina de Vetores de Suporte , Transcriptoma/genética
15.
Genome Biol ; 22(1): 69, 2021 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-33618746

RESUMO

On single-cell RNA-sequencing data, we consider the problem of assigning cells to known cell types, assuming that the identities of cell-type-specific marker genes are given but their exact expression levels are unavailable, that is, without using a reference dataset. Based on an observation that the expected over-expression of marker genes is often absent in a nonnegligible proportion of cells, we develop a method called scSorter. scSorter allows marker genes to express at a low level and borrows information from the expression of non-marker genes. On both simulated and real data, scSorter shows much higher power compared to existing methods.

16.
Phys Chem Chem Phys ; 23(7): 4404-4412, 2021 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-33594400

RESUMO

Critical Casimir force (CCF) is a solvent fluctuation introduced interaction between particles dispersed in a binary solvent. Recently, it has been demonstrated that the CCF induced attraction between particles can trigger particle size-sensitive aggregation, and has thus been used as an efficient way to purify nanoparticles by size. Here, combining small angle neutron scattering and dynamic light scattering, we investigate the effects of size and concentration on this particle size separation method. Increasing the particle concentration does not significantly affect the purification method, but the solvent composition needs to be adjusted for an optimized efficiency. This purification method is further demonstrated to work also very efficiently for systems with particle size ranging from 15 nm to about 50 nm with a very large size polydispersity. These results indicate that for both short-ranged and long-ranged attraction relative to the particle diameter, the CCF introduced particle aggregation is always size sensitive. This implies that particle aggregation is strongly affected by size polydispersity for many colloidal systems. We further propose a method to use light scattering to help identify the temperature range within which this particle purification method can work efficiently instead of using neutron scattering.

17.
ACS Appl Mater Interfaces ; 13(5): 6219-6228, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33499601

RESUMO

Ir-based heterogeneous catalysts for photocatalytic CO2 reduction have rarely been reported and are worthy of investigation. In this work, TiO2 nanosheets with a higher specific surface area and more oxygen vacancies were employed to support Ir metal by impregnation (Imp) and ethylene glycol (EG) reduction methods. In comparison with Ir/TiO2 (Imp) and TiO2, Ir/TiO2 (EG) exhibited excellent photocatalytic performance toward CO2 reduction, especially for CH4 production on account of the oxygen defect of TiO2 and rich surface hydroxyl groups produced from the interaction between TiO2 nanosheets and metallic Ir. In situ ESR suggested that the oxygen defect was significant for CO2 adsorption/activation. Furthermore, metallic Ir was beneficial for photogenerated electron transfer, surface hydroxyl generation, and adsorption of the CO intermediate, generating more available electrons and reducing agents for CH4 production. In situ CO2 DRIFTS confirmed the key synergistic interaction between the oxygen defect and metallic Ir in the photoreduction from CO2 to CH4.

18.
Anal Bioanal Chem ; 413(7): 1955-1966, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33481048

RESUMO

The selective detection of salicylaldehyde skeleton is of great significance in phytochemistry and biological research but rarely reported. In this research, a simple and highly selective "turn-on" fluorescence sensor (CDB-Am) for salicylaldehyde skeleton was developed based on switch of photoinduced electron transfer (PET) and aggregation-induced emission (AIE). CDB-Am bearing amino-cyanodistyrene structure responded to salicylaldehyde in the range of 3.1 to 40 µM with a detection limit of 0.94 µM. The sensing process of formation of Schiff-base adduct CDB-SA was confirmed by 1H NMR, MS, and FT-IR spectra, revealing that a recovered AIE property accounted for the turn-on fluorescence response of CDB-Am and the intramolecular hydrogen bonding played a crucial role in the disruption of PET process. This sensing ability was successfully applied for both fluorescence qualitative test of salicylaldehyde skeleton on TLC analysis and quantitative detection of salicylaldehyde skeleton with good accuracy in the root bark of Periploca sepium, suggesting the extensive applications in phytochemistry and traditional Chinese herbal medicine. Furthermore, CDB-Am exhibited the first excellent fluorescence imaging ability in detecting salicylaldehyde skeleton in a living system. This work supplied a new strategy of preparing a novel "turn-on" fluorescence probe for detecting salicylaldehyde skeleton in complex environments and living bodies.


Assuntos
Aldeídos/análise , Corantes Fluorescentes/análise , Bases de Schiff/análise , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Cromatografia em Camada Delgada , Fluorescência , Humanos , Ligação de Hidrogênio , Radical Hidroxila , Iminas/química , Limite de Detecção , Células MCF-7 , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Medicina Tradicional Chinesa , Microscopia de Fluorescência , Imagem Óptica , Casca de Planta , Raízes de Plantas , Espectrofotometria Ultravioleta , Sais de Tetrazólio/análise , Tiazóis/análise
19.
Biochem Genet ; 59(2): 491-505, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33135088

RESUMO

Chalcone Isomerase (CHI) catalyzes the biosynthesis of flavonoids and secondary metabolism in plants. Currently, there is no systematic analysis of CHIs gene family in Fagaceae which is available. In this study, twenty-two CHI proteins were identified in five species of the Fagaceae family. The CHI superfamily in Fagaceae can be classified into three subfamilies and five groups using phylogenetic analysis, analysis of physicochemical properties, and structural prediction. Results indicated that serine (Ser) and isoleucine (Ile) residues determine the substrate preferred by active Type I Fagaceae CHI, and the chalcone isomerase-like (CHIL) of Fagaceae had active site residues. Adaptive analysis of CHIs showed that CHIs are subject to selection pressure. The active CHI gene of Fagaceae was located in the cytoplasm, and it had the typical gene structure of CHI and contains four exons. All the twenty-two identified CHIs had the conserved domain motif 3, and the different groups had their own structural characteristics. In the process of fatty acid binding protein (FAP) evolution to CHIL and CHI, the physical and chemical properties of proteins also had significant differences in addition to changes in protein functions.


Assuntos
Fagaceae/genética , Liases Intramoleculares/genética , Filogenia , Proteínas de Plantas/genética , Fagaceae/enzimologia
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 248: 119191, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33239250

RESUMO

Previously reported fluorescent sensors for Th4+ experienced emission quenching or generated false positive signal upon aggregate formation in aqueous media. Herein, a simple and novel thorium sensor (CDB-BA) based on cyanodistyrene structure was designed and synthesized, which integrated the highly emitting characteristic of AIE effect and off-on response of PET modulation for the first time to construct the "turn-on" fluorescent probe for Th4+. Besides excellent selectivity, CDB-BA exhibited remarkable fluorescent enhancement which was linearly related to the concentration of Th4+ in the range of 0.25-8 µM. The detection limit was attained 0.074 µM, which was lower than that of most previously reported sensors. The mechanism of tris-chelate complex of CDB-BA with Th4+ was confirmed by mass spectra, IR spectra and DFT calculation. The excellent Th4+ sensing ability of CDB-BA was successfully applied to detecting Th4+ on TLC plates, in real water samples and living-cell imaging. This work suggested that the combination of AIE and PET photophysical mechanism could offer the merits of minimized background and enhanced signal fidelity to develop novel "turn-on" fluorescent probe in complicated aqueous environment and biological research.


Assuntos
Corantes Fluorescentes , Água , Tomografia por Emissão de Pósitrons
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