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1.
Math Biosci Eng ; 16(5): 4382-4398, 2019 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-31499667

RESUMO

Due to both the hidden nature and the irreversibility of Alzheimers disease (AD), it has become the killer of the elderly and is thus the focus of much attention in the medical field. Radiologists compare the predicted brain age with the ground truth in order to provide a preliminary analysis of AD, which helps doctors diagnose AD as early in its development as possible. In this paper, a transfer learning-based method of predicting brain age using MR images and dataset of a public brain was proposed. In order to get the best transfer results, we froze different layers and only fine-tuned the remaining layers. We used three planes of brain MR images together to predict age for the first time and experiment results showed that the proposed method performs better than the state-of-the-art method under mean absolute error metric by 0.6 years. In addition, to explore the relationship between brain MR images of different planes and predicted age accuracy, we used three different planes of brain MR images to predict age respectively for the first time and found that sagittal plane MR images outperformed two other planes in age estimation. Finally, our research identified, the effective regions that contribute to brain age estimation for cognitively normal individuals and for AD patients with deep learning. For AD patients, the effective region is mainly concentrated in the frontal lobe of the brain, verifying the relevant medical conclusions about AD.

2.
Huan Jing Ke Xue ; 40(5): 2460-2470, 2019 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087888

RESUMO

In order to evaluate the potential agricultural risks of soil contaminated by polycyclic aromatic hydrocarbons (PAHs), Lactuca sativa L. was used as a model leaf vegetable plant to investigate the enrichment characteristics of PAHs in the different tissues of Lactuca sativa L, such as its underground parts (GS) and aboveground parts (YS), which were studied through an experiment involving potted cultivation in PAHs contaminated soil that was taken from the agricultural soil around a coking enterprise area. The concentrations of the different PAHs in the soil and plant tissues were analyzed using ultrasonic oscillation extraction and high-performance liquid chromatography-mass spectrometry (GC-MS) analysis methods. The results show that the enrichment of the total PAHs (Σ16PAHs) in the YS is higher than that in the GS. The components enriched in the YS mainly consist of 3-5 ring PAHs, and those in the GS consist of 4-6ring PAHs. The coefficients of the different PAHs enriched in the YS were higher than those of the GS. The enrichment coefficient of anthracene (Ant) was the largest and that of fluoranthene (Fla) was the smallest in the YS, while the enrichment coefficient of benzene[a]pyre (Bap) was the largest and that of Fla was the smallest in the GS. The transfer coefficients of the different PAHs from the GS to the YS were greater than the rate from the initial soil (SS) to the GS; the value is less than 1 from the SS to GS. The correlations and goodness of fit were analyzed for the concentrations of PAHs in the SS, GS and YS. The Σ16PAHs in the SS showed positive correlations with the Σ16PAHs in the YS and in GS. The goodness of fit of the correlation of the PAHs in YS to those in the SS was the largest (R2=1.0), while the goodness-of-fit for the correlation between the GS and SS was lower (R2=0.71). The benzo[a]pyrene equivalent concentration values (Σ16PAHsBapeq) of YS and GS were 11.8 and 12.7 times the maximum value of Bap level allowed in food. The study indicates that contaminated farmland soil would present a high health risk when it was used to grow the plant.


Assuntos
Alface/crescimento & desenvolvimento , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes do Solo/análise , Agricultura , Medição de Risco , Solo
3.
Molecules ; 24(4)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30781736

RESUMO

Hawk tea is a rich and edible resource, traditionally used as a beverage in South China. This drink has many pharmacologic effects, such as acting as an antioxidant and reducing blood sugar and lipids. The objective of this work was to explore the active compound contents, bioactivities and their monthly changes, and optimize the harvest time. In the present study, Hawk tea from each month in 2017 was collected and extracted with 70% (v/v) ethanol. The contents of the total flavonoids and total phenols were determined using the colorimetric method. We determined the contents of seven characteristic active substances-hyperin, isoquercitrin, trifolin, quercitrin, astragalin, quercetin, and kaempferol-using high-performance liquid chromatography. The crude extract was tested for its antioxidant and inhibitory properties on enzymes involved in metabolic syndrome. Specifically, 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), ferric-reducing power assay, and the inhibition capacity test on α-glucosidase and lipase were conducted to determine the antioxidant effect in vitro, as well as the reduction of blood sugar and lipids. Monthly variations in activities and components were determined by numeric analysis and comparison. Correlation analysis revealed that antioxidant effects are significantly correlated with the total flavonoids. The hierarchical cluster analysis of bioactivities and their contents indicates that October and November are the best harvesting months, which differs with the habitual collection of Hawk tea.


Assuntos
Antioxidantes/química , Medicamentos de Ervas Chinesas/química , Síndrome Metabólica/dietoterapia , Chá/química , Antioxidantes/administração & dosagem , China , Medicamentos de Ervas Chinesas/administração & dosagem , Flavonoides/química , Inibidores de Glicosídeo Hidrolases/administração & dosagem , Inibidores de Glicosídeo Hidrolases/química , Humanos , Lipase/antagonistas & inibidores , Lipase/química , Síndrome Metabólica/enzimologia , Fenóis/química , Folhas de Planta/química , alfa-Glucosidases/química , alfa-Glucosidases/efeitos dos fármacos
4.
Chem Biodivers ; 14(12)2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28921887

RESUMO

The fruit (aril) of the endangered genus Taxus plants is an abandoned herbal resource. Traditionally, people enthusiastically focus on its bark, its renewable, tremendous arils fall into the soil with seeds after they are mature. The present research investigated the fruit of two species from the genus Taxus, Taxus chinensis var. mairei, and Taxus media, with regards to their antioxidant and antihyperglycaemic activities, safety, and bioactive constituents. Results showed that T. chinensis var. mairei and T. media both had certain biological activities with T. chinensis var. mairei better in antioxidant activity and T. media better in antihyperglycaemic activity. Correlation analysis revealed that the differences in bioactivities depended on content of their mainly chemical components. The mice acute oral toxicity test indicated that the methanol extracts of the two biotypes of Taxus were safe. And nineteen compounds were tentatively assigned from the two varieties, via tandem mass spectrometry using a LC-ESI-Q-TOF-MS instrument, which included phenols, flavonoids, and terpenes. These results indicate a possible application of Taxus fruit extracts in various fields like in food industry, however, this still needs further investigations.


Assuntos
Extratos Vegetais/química , Taxus/química , Administração Oral , Alanina Transaminase/sangue , Animais , Antioxidantes/administração & dosagem , Antioxidantes/química , Antioxidantes/isolamento & purificação , Aspartato Aminotransferases/sangue , Colesterol/sangue , Cromatografia Líquida de Alta Pressão , Flavonoides/administração & dosagem , Flavonoides/química , Flavonoides/isolamento & purificação , Frutas/química , Frutas/metabolismo , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/química , Hipoglicemiantes/isolamento & purificação , Masculino , Camundongos , Fenóis/administração & dosagem , Fenóis/química , Fenóis/isolamento & purificação , Extratos Vegetais/administração & dosagem , Espectrometria de Massas por Ionização por Electrospray , Taxus/metabolismo , Terpenos/administração & dosagem , Terpenos/química , Terpenos/isolamento & purificação , Testes de Toxicidade Aguda , Ureia/sangue , alfa-Amilases/antagonistas & inibidores , alfa-Amilases/metabolismo
5.
Reproduction ; 153(5): 509-518, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28174321

RESUMO

The ovary of Chinese goose is easily infected by microorganisms because of the mating behaviour in water, which causes decreased laying performance. This study investigated the time course effect of lipopolysaccharide (LPS) on the steroidogenesis and mRNA expression of Toll-like receptors (TLRs), a class of key pattern recognition receptor, in the breeding goose ovary. The laying geese were treated intravenously with LPS for 0, 6, 12, 24 and 36 h, and all birds were slaughtered approximately 8 h after oviposition. The expression levels of TLRs in the white and yellowish follicles, and granulosa and theca layers of hierarchical follicles were examined by real-time PCR. All 10 members of avian TLR family were differentially expressed among the different follicular tissues. Moreover, at 24 and 36 h after LPS treatment, the hierarchical follicle morphological structure was altered, but the expression levels of TLRs were still higher than the control. Furthermore, during LPS treatment period, the expression pattern of TLRs 2A and 4 genes was similar to that of TLR15 in the white follicles, TLRs 1B, 5 and 15 in the yellowish follicles, TLRs 7 and 15 in the granulosa layer, and TLRs 1A, 2B, 3, 7 and 15 in the theca layer, which had a negative correlation with the kinetics of plasma P4 and E2 concentrations. In conclusion, the mechanism by which pathogen infection inhibited goose follicular growth and further decreased egg production may involve a gradually enhanced inflammatory response and reduced endocrine function. This may be due to stimulated TLRs in the ovary.


Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Hormônios Esteroides Gonadais/metabolismo , Lipopolissacarídeos/farmacologia , Ovário/metabolismo , Receptores Toll-Like/metabolismo , Animais , Feminino , Gansos , Células da Granulosa/citologia , Células da Granulosa/efeitos dos fármacos , Células da Granulosa/metabolismo , Folículo Ovariano/citologia , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/metabolismo , Ovário/citologia , Ovário/efeitos dos fármacos , Células Tecais/citologia , Células Tecais/efeitos dos fármacos , Células Tecais/metabolismo , Fatores de Tempo , Receptores Toll-Like/genética
6.
Sci Rep ; 6: 26528, 2016 05 20.
Artigo em Inglês | MEDLINE | ID: mdl-27198490

RESUMO

Ribosomal RNA internal transcribed spacer-1 (ITS1) metabarcoding was used to investigate the distribution patterns of fungal communities and the factors influencing these patterns in subtropical Chinese seas, including the southern and northern Yellow Sea and the Bohai Sea. These seas were found to harbor high levels of fungal diversity, with 816 operational taxonomic units (OTUs) that span 130 known genera, 36 orders, 14 classes and 5 phyla. Ascomycota was the most abundant phylum, containing 72.18% and 79.61% of all OTUs and sequences, respectively, followed by Basidiomycota (19.98%, 18.64%), Zygomycota (1.10%, 0.11%), Chytridiomycota (0.25%, 0.04%) and Rozellomycota (0.12%, 0.006%). The compositions of fungal communities across these three sea regions were found to be vary, which may be attributed to sediment source, geographical distance, latitude and some environmental factors such as the temperature and salinity of bottom water, water depth, total nitrogen, and the ratio of total organic carbon to nitrogen. Among these environmental factors, the temperature of bottom water is the most important driver that governs the distribution patterns of fungal communities across the sampled seas. Our data also suggest that the cold-water mass of the Yellow Sea likely balances competitive relationships between fungal taxa rather than increasing species richness levels.


Assuntos
Código de Barras de DNA Taxonômico/métodos , Fungos/classificação , Sedimentos Geológicos/microbiologia , Microbiologia da Água , China , DNA Fúngico/genética , DNA Ribossômico/genética , DNA Espaçador Ribossômico/genética , Fungos/genética , Metagenoma , Filogenia
7.
Zhongguo Zhong Yao Za Zhi ; 41(22): 4226-4233, 2016 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-28933093

RESUMO

To study the effect of plant protein and animal protein on amino acid metabolism spectrum of Qi and Yin deficiency type 2 diabetic rats. 110 male SD rats were randomly divided into blank group (n=10), diabetic model group (n=20), disease-symptoms group (n=80). The rats of blank group received ordinary feeding, while other groups were fed with high sugar and fat diets. During the whole process of feeding, rats of disease-symptoms group were given with Qingpi-Fuzi (15.75 g•kg⁻¹) once a day through oral administration. Five weeks later, the rats were given with a low dose of STZ (40 mg•kg⁻¹) by intraperitoneal injection to establish experimental diabetic models. Then the models were randomly divided into disease-symptoms group 1 (Qi and Yin deficiency diabetic group, 15.75 g•kg⁻¹), disease-symptoms group 2 (plant protein group, 0.5 g•kg⁻¹), disease-symptoms group 3 (animal protein group, 0.5 g•kg⁻¹), disease-symptoms group 4 (berberine group, 0.1 g•kg⁻¹). The drugs were given for 4 weeks by gavage administration. After 4 weeks of protein intervention, the abdominal aortic blood was collected and serum was isolated to analyze its free amino acid by using AQC pre-column derivatization HPLC and fluorescence detector. Four weeks after the protein intervention, plant protein, animal protein and berberine had no obvious effect on body weight and blood sugar in type 2 diabetic rats. As compared with animal protein group, histidine and proline(P<0.01), serine, glycine, threonine, alanine, tyrosine, valine, methionine, bright+isoleucine, phenylalanine and lysine(P<0.05)changed a lot in rats serum of plant protein group.The results showed that gavage administration of protein would produce effects on amino acid metabolism of Qi and Yin deficiency type 2 diabetic SD rats. Symbolic differential compounds could be found through metabonomics technology, providing experimental basis for early warning of type 2 diabetes and diagnosis of Qi and Yin deficiency syndrome.


Assuntos
Aminoácidos/metabolismo , Diabetes Mellitus Experimental/tratamento farmacológico , Dieta , Medicamentos de Ervas Chinesas/farmacologia , Deficiência da Energia Yin/tratamento farmacológico , Animais , Diabetes Mellitus Tipo 2/tratamento farmacológico , Masculino , Medicina Tradicional Chinesa , Qi , Ratos , Ratos Sprague-Dawley
8.
Zhongguo Zhong Yao Za Zhi ; 41(16): 3027-3031, 2016 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-28920343

RESUMO

To investigate the microbial species, amount changes as well as the isolation and identification of domain strains at different fermentation time points of Pinelliae Rhizoma Fermentata, and provide basis for exploring the mechanism of Pinelliae Rhizoma Fermentata processing. Five samples were chosen at the time points (0, 18, 36, 54, 72 h) of Pinelliae Rhizoma Fermentata processing. Bacteria, mold and yeast from the samples were cultured; their colonies were counted, and the dominant strains were isolated and purified. The dominant bacteria and dominant fungi were identified by 16S rDNA and 26S rDNA sequencing respectively. The results showed that the bacteria count was low with slow and smooth changes in the fermentation process;while mold and yeast grew dramatically after 54 h culturing and reached 1×107 CFU•mL⁻¹ at the end of fermentation. Through the NCBI homology alignment and phylogenetic tree construction, the dominant bacteria were identified as Streptomyces sp., Bacillus pumilus, B. subtilis, B. aryabhattai and other Bacillus sp.; the dominant yeast was identified as Meyerozyma guilliermondii; the dominant mold were identified as Paecilomyces variotii, Byssochlamys spectabilis, and Aspergillus niger in the processing of Pinelliae Rhizoma Fermentata. The results indicated that multiple microbe species, especially yeast and mold, played a role in the fermentation processing of Pinelliae Rhizoma Fermentata. M. guilliermondii, P. variotii, P. variotii and A. niger and Bacillus sp. can be the crucial factors in the processing of Pinelliae Rhizoma Fermentata.


Assuntos
Bactérias/classificação , Medicamentos de Ervas Chinesas/análise , Fermentação , Fungos/classificação , Pinellia/microbiologia , Filogenia , Rizoma/microbiologia
9.
Zhongguo Dang Dai Er Ke Za Zhi ; 17(9): 984-8, 2015 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-26412184

RESUMO

OBJECTIVE: To study the effects of caffeine citrate on myelin basic protein (MBP) expression in the cerebral white matter of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the related mechanism. METHODS: Forty-eight seven-day-old Sprague-Dawley neonatal rats were randomly assigned to 3 groups: sham operation (n=16), HIBD (n=16) and HIBD+caffeine citrate (n=16). The rats in the HIBD and HIBD+caffeine citrate groups were subjected to left common carotid artery ligation, and then were exposed to 80 mL/L oxygen and 920 mL/L nitrogen for 2 hours to induce HIBD. The rats in the sham operation group were only subjected to a sham operation, without the left common carotid artery ligation or hypoxia exposure. Caffeine citrate (20 mg/kg) was injected intraperitoneally before hypoxia ischemia (HI) and immediately, 24 hours, 48 hours and 72 hours after HI. The other two groups were injected intraperitoneally with an equal volume of normal saline at the corresponding time points. On postnatal day 12, the expression of MBP in the left subcortical white matter was detected by immunohistochemistry, and the levels of adenosine A1 receptor mRNA and A2a receptor mRNA in the left brain were detected by real-time PCR. RESULTS: The expression of MBP in the left subcortical white matter in the HIBD group was lower than in the sham operation group (P<0.05). The MBP expression in the HIBD+caffeine citrate group was significantly higher than in the HIBD group, but was still lower than the sham operation group (P<0.05). Real-time PCR showed that the adenosine A1 receptor mRNA expression was significantly higher in the HIBD group than in the sham operation group, and it was significantly lower in the HIBD+caffeine citrate group than in the HIBD group (P<0.05). CONCLUSIONS: Caffeine citrate can improve brain white matter damage following HIBD in neonatal rats and the protection mechanism might be related with the down-regulation of adenosine A1 receptor expression.


Assuntos
Cafeína/farmacologia , Citratos/farmacologia , Hipóxia-Isquemia Encefálica/tratamento farmacológico , Proteína Básica da Mielina/análise , Animais , Animais Recém-Nascidos , Feminino , Hipóxia-Isquemia Encefálica/metabolismo , Hipóxia-Isquemia Encefálica/patologia , Masculino , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Receptor A1 de Adenosina/genética , Receptor A2A de Adenosina/genética , Substância Branca/química
10.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(3): 290-4, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24661524

RESUMO

OBJECTIVE: To investigate the effects of erythropoietin (EPO) on the neuronal proliferation and apoptosis in neonatal rats after infection-induced brain injury and the neuroprotective mechanism of EPO in neonatal rats with infection-induced brain injury. METHODS: Twenty-six two-day-old neonatal rats were randomly divided into 3 groups: control group (intraperitoneally given an equal volume of normal saline), lipopolysaccharide (LPS) group (intraperitoneally given LPS 0.6 mg/kg), and EPO group (intraperitoneally given LPS 0.6 mg/kg and EPO 5 000 U/kg). These groups were injected with respective drugs for 5 consecutive days. Meanwhile, each group was intraperitoneally injected with 5-bromo-2'-deoxyuridine (BrdU) (50 mg/kg) once a day for 5 consecutive days. The expression of BrdU and cleaved Caspase-3 in the hippocampal dentate gyrus was detected by immunohistochemistry at 24 hours after the last injection. RESULTS: The number of neuronal cells in the hippocampal dentate gyrus in the LPS and EPO groups was significantly greater than in the control group (P<0.05), but there was no significant difference between the LPS and EPO groups. The EPO group had a significantly higher number of BrdU-positive cells in the subgranular zone of hippocampal dentate gyrus than the LPS group (51±9 vs 29±6; P<0.05), but a significantly lower number of BrdU-positive cells than the control group (51±9 vs 67±12; P<0.05). The EPO group had a significantly lower number of cleaved Caspase-3-positive cells in the subgranular zone of hippocampal dentate gyrus than the LPS group (27.9±1.5 vs 34.0±1.3; P<0.05), but a significantly higher number of cleaved Caspase-3-positive cells than the control group (27.9±1.5 vs 21.0±1.7; P<0.05). CONCLUSIONS: EPO can promote hippocampal neuronal proliferation and reduce neuronal apoptosis in neonatal rats after infection-induced brain injury.


Assuntos
Apoptose/efeitos dos fármacos , Encefalopatias/tratamento farmacológico , Eritropoetina/farmacologia , Hipocampo/patologia , Neurônios/patologia , Animais , Animais Recém-Nascidos , Encefalopatias/patologia , Bromodesoxiuridina/metabolismo , Caspase 3/metabolismo , Proliferação de Células/efeitos dos fármacos , Eritropoetina/uso terapêutico , Ratos , Ratos Sprague-Dawley
11.
Shanghai Kou Qiang Yi Xue ; 22(4): 368-73, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24100892

RESUMO

PURPOSE: To observe the incidence of osteoclasts during early bone remodeling after orthodontic micro-implant placement. METHODS: Twenty New Zealand rabbits were randomly allotted into 4 groups. One micro-implant was implanted proximal to the epiphyseal plate of the tibia. Animals were sacrificed on day 3, 7, 14 and 28 (n=5). The sequence of histological changes around the micro-implants were evaluated by hematoxylin and eosin (HE) staining. Osteoclasts were identified by TRAP staining. The differences of the number of the osteoclasts among each time point were analyzed by one way ANOVA with SPSS 19.0 software package. RESULTS: After 3 days of implantation, a large number of erythrocytes, inflammatory cells, mesenchymal cells and bone debris were seen at the implant bone interfaces. Few osteoclasts were observed. On day 7, granular woven bone was formed and some osteoclasts were found in the Howship's lacunae. New bone formation and mineralization were apparent on day 14. Meanwhile, large amounts of osteoclasts were found in the latticed woven bone. On day 28, woven trabeculae with lamellate structures connected to lamellar bone and fewer osteoclasts were identified. Semi-quantitative analysis showed that the number of the osteoclasts was at peak on day 14. There were significant differences among each time point (P<0.01). CONCLUSIONS: Osteoclast activity is closely related to bone formation and remodeling after micro-implant insertion.


Assuntos
Remodelação Óssea , Osteoclastos , Animais , Coelhos , Tíbia
12.
Zhongguo Dang Dai Er Ke Za Zhi ; 14(11): 838-41, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23146731

RESUMO

OBJECTIVE: To study risk factors for periventricular-intraventricular hemorrhage (PVH-IVH) in premature infants treated with mechanical ventilation. METHODS: A total of 205 premature infants who were admitted to the neonatal intensive care unit (NICU) and treated with mechanical ventilation between January 2009 and December 2011 were enrolled. They were classified into PVH-IVH and non-PVH-IVH groups according to the results of head ultrasonography performed at 3 to 7 days after birth. Single factor and multivariate logistic regression analysis were used to identify risk factors for PVH-IVH. RESULTS: Single factor analysis indicated 9 factors associated with the development of PVH-IVH, including a gestational age of <32 weeks, a birth weight of <1500 g, intrauterine distress, severe asphyxia, vaginal delivery, maternal perinatal infection, premature rupture of membranes (PROM) at ≥8 hours, mechanical ventilation duration of ≥7 days and ventilator-associated pneumonia (VAP) (P<0.05). Multivariate logistic regression analysis showed that a birth weight of <1500 g (OR=2.665), intrauterine distress (OR=2.177), severe asphyxia (OR=5.653), maternal perinatal infection (OR=4.365) and VAP (OR=2.299) were independent risk factors for the development of PVH-IVH (P<0.05). CONCLUSIONS: Very low birth weight, intrauterine distress, severe asphyxia, maternal perinatal infection and VAP are closely associated with an increased risk of PVH-IVH in premature infants treated with mechanical ventilation. These clinical risk factors should be given more attention in the prevention of PVH-IVH.


Assuntos
Hemorragia Cerebral/etiologia , Doenças do Prematuro/etiologia , Respiração Artificial/efeitos adversos , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Unidades de Terapia Intensiva Neonatal , Modelos Logísticos , Masculino , Pneumonia Associada à Ventilação Mecânica/complicações , Prognóstico , Fatores de Risco
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