Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 987
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Nanotechnology ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32380488

RESUMO

We theoretically investigate the coupling between a single silver nanoparticle and chiral molecular J-aggregates (TDBC). The element of the structure is composed of Ag nanoparticle coated by chiral TDBC. The results show that the coupling between Ag nanoparticle and TDBC can be tuned by the size of Ag nanoparticle. By changing the size of Ag nanoparticle, both the strong coupling effect and the weak coupling effect between Ag nanoparticle and TDBC are achieved. Circular dichroism (CD) spectra of the hybridized structures in both the strong coupling regime and the weak coupling regime present Fano line-shape, which can be represented in the form of (ϵ + q)^2/(ϵ^2 + 1). We also find that CD spectrum in the strong coupling regime is less than the ones in the weak coupling regime. Maximum of CD spectra of the hybridized structure is amplified as 130 times as the one of chiral TDBC in the strong coupling regime, and as 490 times as the one of chiral TDBC in the weak coupling regime, respectively. Much more energies are used to change the resonant wavelength of the hybridized structure in the strong coupling regime, while the energies are mainly used to enhance CD spectrum in the weak coupling regime. Our researches have great potential on molecule detections.

2.
Thyroid ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32380933

RESUMO

BACKGROUND: Thionamides have been extensively used to treat patients with hyperthyroidism worldwide. Recent pharmacovigilance studies have revealed a safety signal between carbimazole or methimazole and pancreatitis. The associated risk remains unclear. METHODS: We identified patients with newly diagnosed acute pancreatitis from 2000 to 2013 as the case group from the Taiwan Longitudinal Health Insurance Database 2000, which contains data from 1996 to 2013. Each patient with acute pancreatitis was matched for age, sex, comorbidities, and cancer with four controls through propensity score matching. A total of 52 patients without matched controls were excluded. Sensitivity analyses including the 52 excluded patients were performed using a matching ratio of 1:2. Odds ratios (ORs) along with 95% confidence intervals (CIs) for the association were estimated using multivariate logistic regression. RESULTS: We included 9,204 and 36,816 patients in the case and control groups, respectively. The proportions of patients who had used thionamides, carbimazole, methimazole, and propylthiouracil were similar in these two groups. In addition, the adjusted OR (95% CI) for the association of acute pancreatitis with thionamides was 1.03 (0.86-1.24), with carbimazole was 0.90 (0.63-1.30), with methimazole was 1.05 (0.84-1.31), and with propylthiouracil was 1.00 (0.74-1.34). The sensitivity analysis results were unchanged. CONCLUSION: We were unable to demonstrate an association between acute pancreatitis and use of thionamides.

3.
Plant Physiol Biochem ; 152: 53-61, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32388420

RESUMO

Silicon (Si) can increase salt tolerance of plants, and previous studies have focused on NaCl stress; whereas in protected facilities, nitrate (but not NaCl) accumulation is one of the major causes of secondary soil salinization. However, information on Si's effect on plant growth under nitrate stress is very limited, and the underlying mechanism is unknown. Here, we investigated Si's effect on plant growth, nitrogen assimilation and chlorophyll synthesis in cucumber. Cucumber seedlings ('Jinyou 1') were subjected to 200 mM nitrate stress without or with addition of 2 mM Si. The results showed that root application, but not foliar application of Si, could improve cucumber growth under nitrate stress. Root addition of Si increased photosynthetic rate and decreased oxidative damage of stressed plants. Under nitrate stress, Si addition decreased the accumulation of nitrate, nitrite and ammonium, and promoted the activities of nitrate reductase, nitrite reductase, glutamine synthase, glutamine-2-oxoglutarate aminotransferase and glutamate dehydrogenase in leaves. The concentrations of glutamic acid, 5-aminolevulinic acid, porphobilinogen and uroporphyrinogen Ⅲ were increased under nitrate stress, while these were decreased by added Si. Added Si increased the levels of chlorophyll and its precursors (protoporphyrin Ⅸ, Mg-protoporphyrin Ⅸ and protochlorophyllide), and expressions of genes encoding enzymes in chlorophyll synthesis (CHLH, POR and CAO) under nitrate stress. These results suggest that Si could improve cucumber growth under nitrate stress by enhancing nitrogen assimilation and chlorophyll synthesis, and imply an application of Si fertilizer in solving secondary soil salinization in protected facilities.

4.
Plant Biotechnol J ; 2020 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-32386262

RESUMO

All plant-interacting microbes must acquire metabolites from their hosts to satisfy nutritional demands for growth. With carbon being crucial for all organisms, sufficient acquisition of sugars from plants is a cardinal task of plant pathogens for successful invasion. Blocking access to host sugars seems to be a promising strategy to control plant diseases. Plant sugar retrieval strengthens plant resistance to pathogens (Yamada et al., 2016). However, it is difficult to discriminate if this is a result of blocking the pathogen's access to sugar, or a disturbance in sugar-mediated signaling in plants (Milne et al., 2019; Moore et al., 2015). Since the identification of UfHXT1 provided the first evidence of sugar uptake in rust fungi (Voegele et al., 2001), many sugar transporters have been identified from different pathogenic fungi (Saitoh et al., 2014; Schuler et al., 2015). However, the effects of sugar starvation on pathogen growth, development and pathogenicity are still unclear.

5.
Am J Chin Med ; 48(3): 651-678, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32349518

RESUMO

Cinobufagin is a Na+/K+-ATPase (NKA) inhibitor with excellent anticancer effects to prolong the survival of patients. The purpose of the present study was to clarify the underlying mechanism of the anticancer effects of cinobufagin using overexpression or inhibition of aurora kinase A (AURKA) signaling. First, high expression of Na+/K+-ATPase alpha 1 subunit (ATP1A1) and AURAK resulted in increased malignant transformation in hepatocellular carcinoma (HCC) patients using the cancer genome atlas (TCGA) data and tissue samples. After treatment with cinobufagin, we successfully screened 202, 249, and 335 changing expression proteins in Huh-7 cells under normal, overexpression, and inhibition of AURKA using tandem mass tags (TMT)-labeled quantitative proteomics coupled to 2D liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics analysis revealed that these molecules were closely associated with chromosome segregation, DNA damage, and regulation of translation processes. We further confirmed that cinobufagin induced DNA damage and chromosome segregation disorders and suppresses translational processing in oncogenes by decreasing the expression of AURKA, mechanistic target of rapamycin kinase (mTOR), p-mTOR, p-extracellular regulated protein kinases (ERK), eukaryotic translation initiation factor 4E (eIF4E), and p-eIF4E, while increasing the expression of p-eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) (S65, T37, T46, T45) and increasing the interaction between eIF4 and 4E-BP1. Our results suggested that cinobufagin performed an antitumor effects in liver cancer cells by inhibiting the AURKA-mTOR-eIF4E axis.

6.
Arch Insect Biochem Physiol ; : e21691, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32410326

RESUMO

In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32368886

RESUMO

Antibody-drug conjugates have shown great promise in active targeting for cancer therapy. The existing chemical techniques for antibody conjugation generally lack efficiency or universality. In this article, a site-specific antibody conjugation was developed by using a mild reaction between a benzoboroxole (BB) functionality and cis-diol moiety of sugar units in the antibody fragment crystallizable region under neutral pH conditions. A BB/PEG/ICG-grafted poly(aspartic acid) comb-like functional polymer was first synthesized and conjugated with transferrin (Tf) to form a transferrin-polymer-drug conjugate [Tf-P(BB)], which showed 120% increase in HepG2 hepatoma (Tf receptor overexpression) cell uptake compared to a nontargeting protein-polymer-drug conjugate [HRP-P(BB)]. The universality of this method was further demonstrated by the enhanced uptake of trastuzumab (anti-Her2 antibody)-polymer-drug conjugates in MCF-7 (295%) and MDA-MB-435S (66.4%) (Her2 positive) cells. The positive charge of the linker had great influence on the targeting ability of the antibody-polymer-drug conjugates. The in vivo studies demonstrated the distinct targeting ability of Tf-P(BB) in the HepG2 xenograft tumor, and the tumor accumulation of the Tf-P(BB) testing group increased by 92% with respect to the control group [HRP-P(BB)]. More significantly, the HepG2 cell uptake amount of the antibody-oriented conjugate [Tf-P'(BB)] was 2.4-fold higher than that of the controlled group [Tf-P'(Hex)]. On the basis of this facile site-specific conjugation method, the conjugates are able to change the antibody species easily against various cancers, while maintaining the antibody integrity and targeting ability.

8.
Foods ; 9(3)2020 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-32245273

RESUMO

Excess summer milk and a lack of product diversity are major problems facing Taiwan's dairy goat industry. Gouda and Mozzarella cheeses made with cow milk are popular products for leisure farms in Taiwan, and they produce a large amount of cheese whey as waste. Our objective is to identify the unstable phenomena of pH-adjusted goat milk through the use of Turbiscan Lab® Expert and to produce ricotta cheeses using cow cheese whey waste and excess goat milk. Delta backscattering (∆BS) profiles and the Turbiscan stability index (TSI) were used to evaluate the stability characteristics of goat milk adjusted to pH 6.7-5.2. The results show coagulation phenomena in skimmed goat milk and sedimentation phenomena in full-fat goat milk, when the pH was adjusted to 5.2. The TSI values of goat milk at pH 5.7 and 5.2 were significantly higher (p < 0.05) than that of a control. Therefore, 80/20 cow cheese whey/skimmed goat milk and 80/20 cow cheese whey/full-fat goat milk mixtures were acidified to pH 5.5 and heated at 90 °C for 30 min to produce ricotta cheeses A and B. The hardness value, moisture, protein, and ash contents of ricotta cheese A were significantly higher (p < 0.05) than that of ricotta cheese B, but no significant difference was found in terms of sensory evaluation.

9.
Neuromolecular Med ; 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32253686

RESUMO

Reactive oxygen species (ROS) are continuously produced as byproducts of aerobic metabolism. Oxidative stress (OS) plays an important role in the occurrence of several neurodegenerative diseases as well as aging because of the accumulation of ROS. Gnaq is a member of G protein α subunits. It has been reported that the expression level of Gnaq in the mouse forebrain cortex was significantly decreased with age in our previous study; therefore, we supposed that Gnaq contributes to attenuate the OS. In this study, we generated a Gnaq-overexpression cell using gene recombinant technique and lentivirus transfection technique in a neuron-like PC12 cell, and investigated whether Gnaq had antioxidant effects in PC12 cells treated with H2O2. The viability of cells, concentration of ROS, Nrf2 nuclear translocation, expression of antioxidant enzymes, activation of NF-κB and apoptosis were compared between Gnaq-PC12 cells and Vector-PC12 cells. Results showed that, compared with Vector-PC12 cells, the antioxidative ability of Gnaq-PC12 cells was significantly improved, while the ROS level in Gnaq-PC12 cells was significantly decreased. Nrf2 nuclear translocation was up-regulated and NF-κB nuclear translocation was down-regulated in Gnaq-PC12 cells after H2O2 treatment. The results suggest that Gnaq plays a crucial role in neuroprotection in PC12 cells. A possible mechanism for this would be that the overexpressed Gnaq enhances the antioxidative effect mediated by Nrf2 signal pathway and inhibits the cellular damaging effect through NF-κB signal pathway.

10.
Pestic Biochem Physiol ; 164: 196-202, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32284127

RESUMO

Lymantria dispar asiatica is a globally distributed herbivorous pest. Avermectin is a highly effective, broad-spectrum insecticide. In this study, fourth instar L. dispar asiatica larvae were exposed to a LC30 dose of avermectin. The structure and function of larval gut microbial community was analyzed to examine how gut microbiota in L. dispar asiatica larvae responded to avermectin stress. Results showed that the structure and function of gut microbial community in L. dispar asiatica larvae were varied by avermectin stress. To be precise, more than half quantity of the observed Optical Taxonomic Units (OTUs) showed significantly different abundances under avermectin stress. Linear discriminant analysis effect size (LEfSe) suggested nine bacterial genera and 12 fungal genera contributed to the different gut microbial community structure in L. dispar asiatica larvae. Gut microbial function classification (PICRUSt and FUNGuild) suggested that three bacterial function categories and a fungal function guild were significantly increased, and two fungal function guilds were significantly decreased by avermectin stress. This study furthers our understanding of the physiology of L. dispar asiatica larvae under avermectin stress, and is an essential step towards future development of potential pesticide targets.


Assuntos
Inseticidas , Lepidópteros , Mariposas , Animais , Ivermectina/análogos & derivados , Larva
11.
J Alzheimers Dis ; 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32280102

RESUMO

Many Alzheimer's disease (AD) patients suffer from persistent neuropathic pain (NP), which is mediated, at least partially, but microglia. Nevertheless, the exact underlying mechanism is unknown. Moreover, a clinically translatable approach through modulating microglia for treating AD-associated NP is not available. Here, in a doxycycline-induced mouse model (rTg4510) for AD, we showed development of NP. We found that the total number of microglia in the CA3 region was not increased, but polarized to pro-inflammatory M1-like phenotype, with concomitant increases in production and secretion of pro-inflammatory cytokines. To examine whether this microglia polarization plays an essential role in the AD-associated NP, we generated an adeno-associated virus (AAV) serotype PHP.B (capable of crossing the blood-brain barrier) carrying shRNA for DNA methyltransferase 1 (DNMT1) under a microglia-specific TMEM119 promoter (AAV-pTMEM119-shDNMT1), which specifically targeted microglia and induced a M2-like polarization in vitro and in vivo in doxycycline-treated rTg4510 mice. Intravenous infusion of AAV-pTMEM119-shDNMT1 induced M2-polarization of microglia and attenuated both AD-associated behavior impairment but also NP in the doxycycline-treated rTg4510 mice. Thus, our data suggest that AD-associated NP may be treated through M2-polarization of microglia.

12.
Small ; : e1907513, 2020 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-32307895

RESUMO

Doped 2,2',7,7'-tetrakis(N,N-di-p-methoxyphenylamine)-9,9'-spirobifluorene (spiro-MeOTAD), which acts as a hole-transporting layer (HTL), endows perovskite solar cells (PSCs) with excellent performance. However, the intrinsically hygroscopic nature of lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) dopants also aggravates the moisture instability of PSCs. In this work, the origins of the moisture instability of spiro-MeOTAD HTLs are explored and strategies to enhance moisture resistance are proposed. After 780 h of aging in air, 52% of the initial power conversion efficiency (PCE) can be sustained by prolonging the mixing time of the precursor solution of spiro-MeOTAD to reduce accumulated LiTFSI. In contrast, only 7% of the initial PCE remains if the precursor solution is mixed briefly. By thermally annealing an HTL to evaporate residual tBP in spiro-MeOTAD, pinholes are completely eliminated and 65% of the initial PCE remains after the same aging time. In this study, the significance of the initial morphology of spiro-MeOTAD HTLs on device stability is analyzed and strategies based on physical morphology for controlling PSC moisture instability induced by HTL dopants are developed.

13.
Adv Mater ; : e2001082, 2020 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-32338405

RESUMO

Graphdiyne (GDY), a newly emerging 2D carbon allotrope, has been widely explored in various fields owing to its outstanding electronic properties such as the intrinsic bandgap and high carrier mobility. Herein, GDY-based photoelectrochemical-type photodetection is realized by spin-coating ultrathin GDY nanosheets onto flexible poly(ethylene terephthalate) (PET) substrates. The GDY-based photodetectors (PDs) demonstrate excellent photo-responsive behaviors with high photocurrent (Pph , 5.98 µA cm- 2 ), photoresponsivity (Rph , 1086.96 µA W- 1 ), detectivity (7.31 × 1010 Jones), and excellent long-term stability (more than 1 month). More importantly, the PDs maintain an excellent Pph after 1000 cycles of bending (4.45 µA cm- 2 ) and twisting (3.85 µA cm- 2 ), thanks to the great flexibility of the GDY structure that is compatible with the flexible PET substrate. Density functional theory (DFT) calculations are adopted to explore the electronic characteristics of GDY, which provides evidence for the performance enhancement of GDY in alkaline electrolyte. In this way, the GDY-based flexible PDs can enrich the fundamental study of GDY and pave the way for the exploration of GDY heterojunction-based photodetection.

14.
Dalton Trans ; 49(17): 5523-5530, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32267264

RESUMO

A series of new W-Co-S clusters have been synthesized utilizing the complex [Et4N][(Tp*)WS3] (Tp* = hydridotris(3,5-dimethylpyrazol-1-yl)borate(1-)) as the synthetic template, by rational control of the stoichiometry of reactants and by employment of a reductant or precipitant as an auxiliary reagent. By solubility control or addition of a precipitant to avoid the involvement of Cl- in the reaction, the cluster [(Tp*)WS3]2Co (2) could be obtained. With different ratios of the Co source to the template, together with the use of an appropriate amount of reducing reagent, clusters [{(Tp*)WS3}2Co]1- (3) and [{(Tp*)WS3}2Co2Cl]1- (4) could be synthesized as their Et4N+ salts, and [{(Tp*)WS3}2Co4Cl2(DMF)]1- (5) could be isolated as its [Co(DMF)6]2+ salt. A common structural feature of these clusters is that they all have a pair of [(Tp*)WS3] templates chelating different numbers of Co atoms to form W-Co-S clusters with distinct nuclearities. The clusters [(Tp*)WS3]2Co (2) and [{(Tp*)WS3}2Co]1- (3) both have one Co atom chelated by two [(Tp*)WS3] templates, and the difference lies in not only the oxidation states but also the fact that the Co atom in 2 adopts an octahedral coordination environment while that in 3 bears a tetrahedral coordination sphere, which means that the [(Tp*)WS3] template is tri-dentate in 2 and bi-dentate in 3. The cluster [{(Tp*)WS3}2Co2Cl]1- (4) has two Co atoms chelated by a pair of [(Tp*)WS3] templates, in which one of the Co atoms is also terminally bound by a Cl- ligand. There are four Co atoms chelated by two [(Tp*)WS3] templates in the cluster [{(Tp*)WS3}2Co4Cl2(DMF)]1- (5), two of which are bound terminally by the Cl- ligands. Studies of redox properties by cyclic voltammetry indicate the existence of two reversible redox pairs related to clusters 2 and 3, respectively.

15.
Cells ; 9(4)2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32244728

RESUMO

The ability to perform highly sensitive and multiplexed in-situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, we here develop an approach using cleavable biotin-conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin-labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin-conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced at least 10-fold, compared with the current in-situ proteomics methods. After imaging, the fluorophore and the biotin unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be profiled in individual cells at the optical resolution. Applying this approach, we have demonstrated that multiple proteins are unambiguously detected in the same set of cells, regardless of the protein analysis order. We have also shown that this method can be successfully applied to quantify proteins in formalin-fixed paraffin-embedded (FFPE) tissues.

16.
BMC Genomics ; 21(1): 315, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32312234

RESUMO

BACKGROUND: Climate change, including higher temperatures (HT) has a detrimental impact on wheat productivity and modeling studies predict more frequent heat waves in the future. Wheat growth can be impaired by high daytime and nighttime temperature at any developmental stage, especially during the grain filling stage. Leaf chlorophyll content, leaf greenness, cell membrane thermostability, and canopy temperature have been proposed as candidate traits to improve crop adaptation and yield potential of wheat under HT. Nonetheless, a significant gap exists in knowledge of genetic backgrounds associated with these physiological traits. Identifying genetic loci associated with these traits can facilitate physiological breeding for increased yield potential under high temperature stress condition in wheat. RESULTS: We conducted genome-wide association study (GWAS) on a 236 elite soft wheat association mapping panel using 27,466 high quality single nucleotide polymorphism markers. The panel was phenotyped for three years in two locations where heat shock was common. GWAS identified 500 significant marker-trait associations (MTAs) (p ≤ 9.99 × 10- 4). Ten MTAs with pleiotropic effects detected on chromosomes 1D, 2B, 3A, 3B, 6A, 7B, and 7D are potentially important targets for selection. Five MTAs associated with physiological traits had pleiotropic effects on grain yield and yield-related traits. Seventy-five MTAs were consistently expressed over several environments indicating stability and more than half of these stable MTAs were found in genes encoding different types of proteins associated with heat stress. CONCLUSIONS: We identified 500 significant MTAs in soft winter wheat under HT stress. We found several stable loci across environments and pleiotropic markers controlling physiological and agronomic traits. After further validation, these MTAs can be used in marker-assisted selection and breeding to develop varieties with high stability for grain yield under high temperature.

17.
J Clin Neurosci ; 75: 45-51, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32229069

RESUMO

Due to the varied overall survival (OS), limited studies focus on the factors that affect the prognosis for lower grade glioma patients (LGGs) with MGMT promoter methylated. A total of 579 samples (TCGA LGGs 456; CGGA LGGs 123) were included to identify potential genes for LGGs with MGMT promoter methylated. All bioinformatics analyses were conducted using SPSS software and GraphPad Prism 6. Based on COX regression analysis, we established a four-gene signature (ALDOC, APOBEC3C, ANXA1 and ARPP21) and divided LGGs into two groups based on median risk score. The OS of LGGs in high risk group was shorter than low risk group (P < 0.0001). Furthermore, the OS in high risk group were shorter than low risk group in Grade II and III, respectively (P = 0.0003; P = 0.0104). It showed that the signature was an independent prognosis factor on multivariate Cox regression analysis (P = 0.033). Patients in high group tended to displayed high grade (GIII), IDH1 wild type and mesenchymal subtype preference. Four-gene signature was discovered for LGGs with MGMT promoter methylated. Our findings suggested that the four genes could serve as prognostic biomarkers.

18.
Sci Rep ; 10(1): 7023, 2020 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-32341406

RESUMO

An integration of field-based phenotypic and genomic data can potentially increase the genetic gain in wheat breeding for complex traits such as grain and biomass yield. To validate this hypothesis in empirical field experiments, we compared the prediction accuracy between multi-kernel physiological and genomic best linear unbiased prediction (BLUP) model to a single-kernel physiological or genomic BLUP model for grain yield (GY) using a soft wheat population that was evaluated in four environments. The physiological data including canopy temperature (CT), SPAD chlorophyll content (SPAD), membrane thermostability (MT), rate of senescence (RS), stay green trait (SGT), and NDVI values were collected at four environments (2016, 2017, and 2018 at Citra, FL; 2017 at Quincy, FL). Using a genotyping-by-sequencing (GBS) approach, a total of 19,353 SNPs were generated and used to estimate prediction model accuracy. Prediction accuracies of grain yield evaluated in four environments improved when physiological traits and/or interaction effects (genotype × environment or physiology × environment) were included in the model compared to models with only genomic data. The proposed multi-kernel models that combined physiological and genomic data showed 35 to 169% increase in prediction accuracy compared to models with only genomic data included when heading date was used as a covariate. In general, higher response to selection was captured by the model combing effects of physiological and genotype × environment interaction compared to other models. The results of this study support the integration of field-based physiological data into GY prediction to improve genetic gain from selection in soft wheat under a multi-environment context.

19.
Microb Pathog ; 144: 104201, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32325238

RESUMO

Brucellosis is a zoonotic infectious disease caused by Brucella infection. MarR-family transcription factors are closely related to diverse physiological functions necessary for many pathogens adaptation to environmental changes. However, whether the MarR-family transcription factors are involved in virulence, mediated inflammatory responses and regulated virulence gene expression in the intracellular pathogen Brucella are still unknown. Therefore, we created a 2308ΔMarR6 mutant of B. abortus 2308 (S2308). Virulence and inflammatory cytokines assays were performed using a murine macrophage cell line (RAW 264.7). We also performed chromatin immunoprecipitation of MarR6 followed by next-generation sequencing (ChIP-seq). The results showed that 2308ΔMarR6 was significantly reduced survival capability in RAW 264.7. After the macrophages were infected with 2308ΔMarR6, the levels of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), interleukin-6 (IL-6), interleukin-12 (IL-12), interferon-gamma (IFN-γ) and macrophage chemoattractant protein-1 (MCP-1) were decreased and were significantly lower than that for the S2308-infected group, indicating that the 2308ΔMarR6 mutant could reduce the secretion of inflammatory cytokines. Furthermore, we detected 122 intergenic ChIP-seq peaks of MarR6 binding distributed across the Brucella genome. Taken together, the research has recorded valuable data about MarR6. Our findings are of great significance in elucidating the function of MarR6.

20.
Cell Commun Signal ; 18(1): 44, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32164705

RESUMO

BACKGROUND: Sialic acids are widely distributed in animal tissues, and aberrantly expressed in a variety of cancer types. High expression of sialic acid contributes to tumor aggressiveness by promoting cell proliferation, migration, angiogenesis, and metastasis. Sialidases are responsible for removal of sialic acids from glycoproteins and glycolipids. METHODS: N-glycomics of bladder cancer cells were detected by MALDI-TOF mass spectrometry. Sialic acid modification in bladder cancer tissue was determined by lectin blot. The down-regulation of NEU1 in bladder cancer cells was determined by high resolution liquid chromatography mass spectrometry (HR LC-MS). The effects of sialidase NEU1 expression on proliferation and apoptosis of human bladder cancer cells were examined by western blot, RT-PCR, confocal imaging and flow cytometry. Moreover, the function of sialic acids on fibronectin-integrin α5ß1 interaction were assayed by immunoprecipitation and ELISA. The importance of NEU1 in tumor formation in vivo was performed using BALB/c-nu mice. Expression of NEU1 in primary human bladder cancer tissue samples was estimated using bladder cancer tissue microarray. RESULTS: (1) Downregulation of NEU1 was primarily responsible for aberrant expression of sialic acids in bladder cancer cells. (2) Decreased NEU1 expression was correlated with bladder cancer progression. (3) NEU1 overexpression enhanced apoptosis and reduced proliferation of bladder cancer cells. (4) NEU1 disrupted FN-integrin α5ß1 interaction and deactivated the Akt signaling pathway. (5) NEU1 significantly suppressed in vivo tumor formation in BALB/c-nu mice. CONCLUSIONS: Our data showed that NEU1 inhibited cancer cell proliferation, induced apoptosis, and suppressed tumor formation both in vitro and in vivo, by disrupting interaction of FN and integrin ß1 and inhibiting the Akt signaling pathway. Our observations indicate that NEU1 is an important modulator of the malignant properties of bladder cancer cells, and is a potential therapeutic target for prognosis and treatment of bladder cancer. Video Abstract.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA