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1.
J Cell Physiol ; 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32020591

RESUMO

Long noncoding RNAs (lncRNAs) have been reported to dysregulate and involve in the pathology of hepatocellular carcinoma (HCC). Nonetheless, the functional role of lncRNA T cell leukemia/lymphoma 6 (TCL6) and its underlying mechanism in HCC remain unclear. Herein, we analyzed the expression of TCL6 and elucidated its mechanistic involvement in HCC. Bioinformatics analyses indicated TCL6 was evidently downregulated in HCC tissues compared with normal controls. TCL6 was downregulated while microRNA-106a-5p (miR-106a-5p) was upregulated in HCC cell lines. Moreover, knockdown or overexpression of TCL6 significantly raised or diminished the expression level of miR-106a-5p in HCC cells, similar to the effect of miR-106a-5p on TCL6 expression. Functionally, TCL6 inhibited the proliferative, migratory, and invasive potentials of HCC cells as analyzed by cell counting kit-8, scratch wound healing, and transwell assays, respectively. Conversely, miR-106a-5p exerted an opposite effect on the proliferative, migratory, and invasive potentials of HCC. RNA immune precipitation and luciferase reporter assays revealed TCL6 directly bound to miR-106a-5p and luciferase reporter assay verified phosphatase and tensin homolog (PTEN) was a target gene of miR-106a-5p. Mechanistically, TCL6 knockdown evidently reduced PTEN expression at both messenger RNA and protein levels, and miR-106a-5p inhibitor partially rescued this reduction effect in HCC cells. Additionally, western blot assays demonstrated miR-106a-5p downregulation or TCL6 overexpression promoted the protein level of PTEN, and suppressed the phosphorylation level of AKT, the protein level of phosphatidylinositol 3-kinase (PI3K). Collectively, these results revealed TCL6 as a tumor-suppressive lncRNA regulates PI3K/AKT signaling pathway via directly binding to miR-106a-5p in HCC. This mechanism provides a theoretical basis for HCC pathogenesis and a potential therapeutic strategy for HCC treatment.

2.
Respiration ; : 1-5, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32050197

RESUMO

Mediastinal biopsy is essential for the clinical diagnosis of mediastinal disease. Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a well-established approach for obtaining diagnostic samples from mediastinal masses or enlarged lymph nodes which is proven to be minimally invasive and effective. However, the insufficiency of intact samples acquired might restrict the diagnostic efficacy of EBUS-TBNA for mediastinal lesions such as rare malignancy and granulomatous disorder. We here present an EBUS-guided approach for the cryobiopsy of mediastinal diseases that is capable of providing larger amounts of intact tissue with few observed complications.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31981375

RESUMO

Cynomorium songaricum Rupr is widely known in China as a traditional herbal medicine. In this study, single-factor experiments and response surface methodology were used to optimize the extraction of Cynomorium songaricum Rupr glycoprotein (CSG). The results show that a maximum glycoprotein yield of 6.39 ± 0.32% was achieved at a ratio of solid to liquid 32:1 for 4.2 H at 52 °C. Then, the IR, monosaccharide composition, amino acid composition, type of glycopeptide linkage, and average molecular weight of CSG-1 purified from CSG were characterized. The results indicate that CSG-1 presented the characteristic absorption peak of polysaccharide and protein, including four monosaccharides and 17 amino acids, had O-linked glycopeptide bonds, Mw , Wn , Mw /Mn , Mp , and the z-average were 5.343 × 106 , 3.203 × 106 , 1.668, 8.911 × 106 , and 6.948 × 106 , respectively. Besides, CSG-1 solution was described by the Herschel-Bulkley model and it behaved as a shear-thinning fluid. Also, under a frequency sweep the moduli G' and G″ both increased with increasing CSG-1 concentration and the CSG-1 dispersions had weak thermal stability over the temperature sweep. These results provide a scientific basis for the further study of Cynomorium songaricum Rupr.

4.
Hum Gene Ther ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-31910043

RESUMO

Applied Genetic Technologies Corporation (AGTC) is developing a recombinant adeno-associated virus (rAAV) vector AGTC-501, also designated rAAV2tYF-GRK1-hRPGRco, to treat X-linked retinitis pigmentosa (XLRP) in patients with mutations in the retinitis pigmentosa GTPase regulator (RPGR) gene. The vector contains a codon-optimized human RPGR cDNA (hRPGRco) driven by a photoreceptor-specific promoter (G protein-coupled receptor kinase 1 [GRK1]), and is packaged in an AAV2 capsid variant with three surface tyrosine residues changed to phenylalanine (AAV2tYF). We conducted a toxicity and efficacy study of this vector administered by subretinal injection in the naturally occurring RPGR mutant (X-linked progressive retinal atrophy 2 [XLPRA2]) dog model. Sixteen RPGR mutant dogs divided into four groups of three to five animals each received either a subretinal injection of 0.07 mL of AGTC-501 at low (1.2 × 1011 vector genome [vg]/mL), mid (6 × 1011 vg/mL), or high dose (3 × 1012 vg/mL), or of vehicle control in the right eye at early-stage disease. The left eye remained untreated. Subretinal injections were well tolerated and were not associated with systemic toxicity. Electroretinography, in vivo retinal imaging, and histological analysis showed rescue of photoreceptor function and structure in the absence of ocular toxicity in the low- and mid-dose treatment groups when compared with the vehicle-treated group. The high-dose group showed evidence of both photoreceptor rescue and posterior segment toxicity. These results support the use of AGTC-501 in clinical studies with patients affected with XLRP caused by RPGR mutations and define the no-observed-adverse-effect level at 6 × 1011 vg/mL.

5.
Molecules ; 25(2)2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31952126

RESUMO

Tyrosinase inhibitors with excellent inhibitory activities and lower side effects have promising applications in the fields of medicine, agriculture, food sciences and cosmetics. In this study, a method for simultaneous separation and determination of six target compounds (puerarin, daidzin, genistein, daidzein, genistin, and formononetin) in Puerariae Lobatae Radix was established by cloud point extraction (CPE) and concentration combined with high performance liquid chromatography (HPLC). To achieve high extraction yields, an ultrasound-assisted extraction method was developed based on a salt-modified Triton X-100 system. The optimal extraction conditions are: surfactant Triton X-100 concentration 0.07 g/mL, liquid-solid ratio 80:1 (mL/g), NaCl addition amount 0.6 g, equilibrium time 40 min, equilibrium temperature 70 °C. Under the optimal conditions, the total maximum extraction yield of the six target isoflavones reached 8.92 mg/g. Using l-tyrosine and l-dopa as substrates, the effects of puerarin on the monophenolase and diphenolase activity of tyrosinase activity were investigated by the enzyme kinetics method. The results showed that puerarin inhibited monophenolase activity with an IC50 of 0.537 mg/mL and activated diphenolase activity. The inhibition type of puerarin on monophenolase and the activation type of puerarin on diphenolase were analyzed by Lineweaver-Burk plots which show that puerarin showed mixed inhibition on monophenolase and mixed activation on diphenolase. Therefore, puerarin can be used as both a tyrosinase inhibitor and a tyrosinase activator.

6.
J Org Chem ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31994395

RESUMO

An unprecedented method for the palladium (II)-catalyzed direct halogenation of benzothiadiazole derivatives is reported here, which would allow the rational tuning of their electronic properties by further transformations. The key strategy is the combination of the double functions of benzothiadiazole as both an important moiety of functional materials and modifiable directing group. Various mono/poly-chlorinated, brominated or iodinated benzothiadiazole products could be prepared efficiently which can be converted into a wide range of valuable polyaromatic compounds with potential appli-cations in material chemistry.

7.
Infect Immun ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31988176

RESUMO

Streptococcus suis (S. suis) is an emerging zoonotic agent that causes streptococcal toxic shock-like syndrome (STSLS) and meningitis in humans, with high mortality and morbidity. The pathogenesis of both STSLS and CNS infections caused by S. suis is not well understood. TRIM32, a member of the tripartite motif (TRIM) protein family, has been reported to regulate host inflammatory responses. In this study, we show that TRIM32 deficiency significantly reduced the level of bacteremia and the production of proinflammatory cytokines following severe S. suis infection, protecting infected mice from STSLS. The influence of TRIM32 gene deletion on a range of processes known to be involved in S. suis meningitis was also examined. Both bacterial loads and indications of brain hemorrhage were reduced in infected Trim32-/- mice compared with infected wild-type (WT) controls. We also found that TRIM32 deficiency increased the permeability of the blood-brain barrier (BBB) and the recruitment of inflammatory monocytes during the early course of S. suis infection, potentially limiting the development of S. suis meningitis. Our results suggest that TRIM32 sensitizes S. suis-induced infection via innate immune response regulation.

8.
J Hazard Mater ; 385: 121509, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31708288

RESUMO

Degradation of brominated flame retardants (BFRs) in waste printed circuit boards (WPCBs) occurred due to mechanical force during the crushing process. In this study, a planetary ball-milling simulation experiment was designed to explore the mechanochemical debromination process of BFRs in WPCBs. The results showed that CaO had a better debromination performance than MgO and the mixture of Fe + SiO2, and high revolution speed and low mass ratio of WPCBs to CaO promoted the degradation of BFRs. After milling for 1 h, the particle size distribution was stable while the debromination efficiency increased with the increase of milling time. Ball milling promoted the migration of bromine from the inside to the new surface of WPCBs powder, and submicron particles adhered to the micron size aggregates. The polybrominated diphenyl ethers (PBDEs) detection showed that the concentrations of most PBDE congeners decreased with the increase of milling time, and a possible degradation pathway was proposed according to the experimental results. All the results provided new data for the mechanism of degradation of BFRs in WPCBs during the mechanical crushing process.

9.
J Hazard Mater ; 385: 121508, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31732335

RESUMO

Z-scheme heterojunction can not only promote the separation of photogenerated carriers, but also retain the strong redox potential of the system, which would greatly improve the photocatalytic performance of catalyst. Herein, a Z-scheme AgI/Bi4V2O11 heterojunction photocatalyst was prepared by a hydrothermal process combined with in situ coprecipitation process. Multiple techniques were employed to investigate the morphology, composition, chemical and electronic properties of the as-prepared samples. The obtained Z-scheme AgI/Bi4V2O11 heterojunction photocatalyst exhibited remarkably enhanced photocatalytic performance towards sulfamethazine (SMZ) degradation under visible light irradiation. Especially, the 20 wt% AgI/Bi4V2O11 composites exhibited the highest photocatalytic activity for sulfamethazine (SMZ) degradation and 91.47% SMZ would be eliminated within 60 min. In comparison with NO3- and SO42-, the presence of Cl- and HCO3- presented more obviously inhibition effects on SMZ degradation. The possible degradation pathways of SMZ were speculated by identifying degradation intermediates. O2-, h+ and OH all involved in the photocatalytic degradation SMZ. The highly enhanced photocatalytic performance might be attributed to form Z-scheme junction between AgI and BVO, which are conducive to the efficient charges separation and maintain high redox potential. This work enriches Bi4V2O11-based Z-scheme heterojunction photocatalytic system and provides a reference for the preparation of effective Z-scheme junction photocatalysts.

10.
Clin Otolaryngol ; 45(1): 47-54, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31623021

RESUMO

OBJECTIVE: Neutrophil infiltration in patients with sinonasal inverted papilloma (SNIP) is significantly high. Whether IL-17, which is a potent factor mediating neutrophilic inflammation, is involved in the neutrophilic phenotype of SNIP is investigated in the current study. STUDY DESIGN: Laboratorial study. PARTICIPANTS: Nasal papilloma and inferior turbinate were collected from patients with SNIP (n = 50) and control subjects with septal deviation (n = 15). METHODS: IL-17 + cells were evaluated in tissues obtained from patients with SNIP and control subjects with septal deviation, by immunohistochemistry and flow cytometry. MAIN OUTCOME MEASURES: The IL-17 + cells were mainly localised in mononuclear cells and neutrophils, and were up-regulated in the SNIP samples compared with those in the controls. The IL-17 + T-cell subsets mainly included CD4+ (Th17, 60.0%) and CD8+ (Tc17, 30.0%), and both subsets were enhanced in the SNIP samples than controls. The total level of IL-17 + cells was significantly correlated with neutrophil infiltration in the SNIP tissues. Furthermore, the SNIP homogenates could significantly promote IL-17 production in peripheral blood mononuclear cells. CONCLUSIONS: An increase in IL-17 + cells is evident in SNIP and may be involved in neutrophil infiltration in local tissues. IL-17 could be a potential therapeutic target to relieve the neutrophilic pathological change in SNIP.

11.
Orthod Craniofac Res ; 23(1): 1-15, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31452316

RESUMO

To evaluate and compare the effectiveness of orthopaedic treatment for Class III malocclusions using skeletal anchorage or a rapid maxillary expander for maxillary protraction. Electronic databases, including PubMed, EMBASE, Cochrane Library and Web of Science, were searched for randomized controlled trials (RCTs) and non-randomized clinical trials (CCTs) for orthopaedic treatment of Class III malocclusions. Five interventions were studied: a facemask with a maxillary temporary anchorage device (MTAD), a bone-anchored rapid maxillary expansion (BARME), a rapid maxillary expansion (RME), an alternate rapid maxillary expansion and contraction (Alt-RAMEC), and a bone-anchored intermaxillary traction (BAIMT). Eight outcomes (SNA, SNB, ANB, overjet, SN-GoGn, ANS-Me, IMPA (L1-MP), and U1-PP) were statistically polled. We conducted network meta-analysis using R statistical software with the GeMTC package. Twenty-five studies met the inclusion criteria. Compared with the RME group, the Alt-RAMEC group (mean difference (MD): 1.3; 95% credibility interval (CrI): 0.26, 2.3) and MTAD group (MD: 0.85; 95% CrI: 0.065, 1.6) showed a better effect on ANB in CCTs. Regarding the vertical relationship, the BAIMT group (MD: -2.2; 95% CrI: -5.2, 0.73) showed a smaller effect regarding increasing the vertical dimension of ANS-Me. The RME, MTAD and Alt-RAMEC group showed a higher ability to decrease the angle of L1-MP. The Alt-RAMEC and MTAD protocol have a higher possibility to obtain a skeletal and tooth effect in sagittal relationships. The BAIMT protocol can acquire a better skeletal effect in sagittal relationships with less vertical and dental changes. More well-designed RCTs are needed to ensure that the conclusion is reliable.


Assuntos
Má Oclusão de Angle Classe III , Ortopedia , Teorema de Bayes , Cefalometria , Aparelhos de Tração Extrabucal , Humanos , Maxila , Meta-Análise em Rede , Técnica de Expansão Palatina
12.
Hum Gene Ther ; 31(1-2): 80-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31544533

RESUMO

Both subretinal dosing and intravitreal (IVT) dosing of adeno-associated virus (AAV) in higher species induce mild and transient inflammatory responses that increase with dose. Foreign protein and foreign DNA are known inducers of inflammation, which is also true in the immune-privileged ocular environment. We explored which component(s) of AAV vectors, viral capsid, or viral DNA drive inflammatory responses. Recombinant AAV with three tyrosine to phenylalanine substitutions in the capsid of AAV serotype 2 (rAAV2tYF), and with a generic ubiquitous promoter (cytomegalovirus [CMV]) controlling the expression of humanized green fluorescent protein (hGFP), was processed to enrich for AAV capsids containing genome (full capsids), capsids without genome (empty capsids), and residual material. Nonhuman primate eyes were injected by IVT in both eyes. During in-life, ocular inflammation and development of neutralizing antibodies (NAb) were measured. Following termination, lymph node immunophenotyping was performed, vitreous was processed for cytokine and RNAseq analyses, and ocular sections were assessed for transgene expression (by in situ hybridization) and histopathology. IVT dosing of AAV vectors transiently raised cellular inflammation in the aqueous and induced a more sustained inflammation in the vitreous. Lowering the total capsid dose by removing empty AAV capsids reduced inflammation and improved viral transduction. IVT dosing of AAV induced systemic NAb to AAV irrespective of the vector preparation. Similarly, lymph node immunophenotyping revealed identical profiles irrespective of viral preparation used for dosing. Immune cells in the vitreous were identified based on RNAseq analysis. Three months postdose, cytokine levels were low, indicative of minimal levels of inflammation in agreement with histopathological assessment of the retina.

13.
Zool Res ; 41(1): 3-19, 2020 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-31840949

RESUMO

Hypobaric hypoxia (HH) exposure can cause serious brain injury as well as life-threatening cerebral edema in severe cases. Previous studies on the mechanisms of HH-induced brain injury have been conducted primarily using non-primate animal models that are genetically distant to humans, thus hindering the development of disease treatment. Here, we report that cynomolgus monkeys ( Macaca fascicularis) exposed to acute HH developed human-like HH syndrome involving severe brain injury and abnormal behavior. Transcriptome profiling of white blood cells and brain tissue from monkeys exposed to increasing altitude revealed the central role of the HIF-1 and other novel signaling pathways, such as the vitamin D receptor (VDR) signaling pathway, in co-regulating HH-induced inflammation processes. We also observed profound transcriptomic alterations in brains after exposure to acute HH, including the activation of angiogenesis and impairment of aerobic respiration and protein folding processes, which likely underlie the pathological effects of HH-induced brain injury. Administration of progesterone (PROG) and steroid neuroprotectant 5α-androst-3ß,5,6ß-triol (TRIOL) significantly attenuated brain injuries and rescued the transcriptomic changes induced by acute HH. Functional investigation of the affected genes suggested that these two neuroprotectants protect the brain by targeting different pathways, with PROG enhancing erythropoiesis and TRIOL suppressing glutamate-induced excitotoxicity. Thus, this study advances our understanding of the pathology induced by acute HH and provides potential compounds for the development of neuroprotectant drugs for therapeutic treatment.


Assuntos
Androstanóis/farmacologia , Hipóxia/veterinária , Macaca fascicularis , Doenças dos Macacos/prevenção & controle , Progesterona/farmacologia , Transcriptoma , Androstanóis/administração & dosagem , Animais , Encefalopatias/prevenção & controle , Encefalopatias/veterinária , Cálcio/metabolismo , Regulação da Expressão Gênica , Hipóxia/patologia , Leucócitos/metabolismo , Masculino , Fármacos Neuroprotetores/farmacologia , Pressão , Progesterona/administração & dosagem
14.
J Hazard Mater ; 383: 121234, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31563045

RESUMO

Pyrolysis is an important pre-treatment technology for pyrometallurgy, which could reduce pollution and recover materials from waste printed circuit boards (WPCBs). However, present studies on mechanism of pyrolysis were insufficient, which results in the unclear of controlling reaction rate and inhibiting side reaction. To further develop pyrolysis technology, the in-depth research on the pyrolysis mechanism is necessary. In this study, we investigated the thermal decomposition process and pyrolysis pathways from macromonomers to products of WPCBs. The results showed that HBr was produced at the initial stage of pyrolysis. Then, the resin body depolymerized into macromonomers, followed by random rupture and free radical reactions to form pyrolysis products. Besides, possible mechanism for bisphenol A thermal decomposing was analyzed by bond energy. The results suggested that methyl groups in bisphenol A would be preferentially removed because of low bond energy. The six possible pathways may occur simultaneously when energy sufficient. Moreover, the mechanism function was determined by Skvára-Sesták method as: G(α)=-ln 1-α2, which indicated pyrolysis reaction agreed with the model of random nucleation followed by random growth. This study provided the theoretical basis for pollution control, process optimization and reactor design of WPCBs pyrolysis.

15.
J Hazard Mater ; 382: 121038, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31450210

RESUMO

Waste printed circuit boards mounted with electronic components (WPCB-ECs) are generated from electronic waste dismantling and recycling process. Air-borne pollutants, including particulate matter (PM) and volatile organic compounds (VOCs), can be released during thermal treatment of WPCB-CEs. In this study, organic substances from WPCB-ECs were pyrolyzed by both thermo-gravimetric analysis (TGA) and in a quartz tube furnace. We discovered that board resin and solder coating were degraded in a one-stage process, whereas capacitor scarfskin and wire jacket had two degradation stages. Debromination of brominated flame retardants occurred, and HBr and phenol were the main products during TGA processing of board resin. Dehydrochlorination occurred, and HCl, benzene and toluene were detected during the pyrolysis of capacitor scarfskin. Benzene formation was found only in the first degradation stage (272-372 °C), while toluene was formed both in the two degradation stages. PM with bimodal mass size distributions at diameters of 0.45-0.5 and 4-5 µm were emitted during heating WPCB-ECs. The PM number concentrations were highest in the size ranges of 0.3-0.35 µm and 1.6-2 µm. The research produced new data on pollutant emissions during thermal treatment of WPCB-ECs, and information on strategies to prevent toxic exposures that compromise the health of recyclers.

16.
Nature ; 577(7790): 416-420, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31875850

RESUMO

Humoral immune responses to immunization and infection and susceptibilities to antibody-mediated autoimmunity are generally lower in males1-3. However, the mechanisms underlying such sexual dimorphism are not well understood. Here we show that there are intrinsic differences between the B cells that produce germinal centres in male and female mice. We find that antigen-activated male B cells do not position themselves as efficiently as female B cells in the centre of follicles in secondary lymphoid organs, in which germinal centres normally develop. Moreover, GPR174-an X-chromosome-encoded G-protein-coupled receptor-suppresses the formation of germinal centres in male, but not female, mice. This effect is intrinsic to B cells, and correlates with the GPR174-enhanced positioning of B cells towards the T-cell-B-cell border of follicles, and the distraction of male, but not female, B cells from S1PR2-driven follicle-centre localization. Biochemical fractionation of conditioned media that induce B-cell migration in a GPR174-dependent manner identifies CCL21 as a GPR174 ligand. In response to CCL21, GPR174 triggers a calcium flux and preferentially induces the migration of male B cells; GPR174 also becomes associated with more Gαi protein in male than in female B cells. Male B cells from orchidectomized mice exhibit impaired GPR174-mediated migration to CCL21, and testosterone treatment rescues this defect. Female B cells from testosterone-treated mice exhibit male-like GPR174-Gαi association and GPR174-mediated migration. Deleting GPR174 from male B cells causes more efficient positioning towards the follicular centre, the formation of more germinal centres and an increased susceptibility to B-cell-dependent experimental autoimmune encephalomyelitis. By identifying GPR174 as a receptor for CCL21 and demonstrating its sex-dependent control of B-cell positioning and participation in germinal centres, we have revealed a mechanism by which B-cell physiology is fine-tuned to impart sexual dimorphism to humoral immunity.

17.
PeerJ ; 7: e8190, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31803544

RESUMO

Cold stress is one of the major abiotic stresses that limit crop production. The ICE-CBF-COR pathway is associated with cold stress response in a wide variety of crop species. However, the ICE-CBF-COR genes has not been well characterized in wheat (Triticum aestivum). This study identified, characterized and examined the expression profiles of the ICE, CBF and COR genes for cold defense in wheat. Five ICE (inducer of CBF expression) genes, 37 CBF (C-repeat binding factor) genes and 11 COR (cold-responsive or cold-regulated) genes were discovered in the wheat genome database. Phylogenetic trees based on all 53 genes revealed that CBF genes were more diverse than ICE and COR genes. Twenty-two of the 53 genes appeared to include 11 duplicated pairs. Twenty rice (Oryza sativa) genes and 21 sorghum (Sorghum bicolor) and maize (Zea mays) genes showed collinearity with the wheat ICE, CBF and COR genes. Transcriptome data and qRT-PCR analyses revealed tissue-specific expression patterns of the ICE, CBF and COR genes, and identified similarities in the expression pattern of genes from the same family when subjected to drought, heat, drought plus heat, and cold stress. These results provide information for better understanding the biological roles of ICE, CBF, COR genes in wheat.

18.
Brachytherapy ; 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31837988

RESUMO

PURPOSE: The aim of the study was to compare the dose distributions of combined intracavitary and interstitial (IC/IS) brachytherapy with 3-catheter IC brachytherapy in treating locally advanced (stage IIB) cervical cancer. METHODS AND MATERIALS: In total, 46 patients were included, each with stage IIB cervical cancer, local lesion sizes ≥5 cm, and tumors that had not regressed after 45 Gy/25 F external intensity-modulated radiotherapy. To identify the dosimetric advantage of delivering a local boost to high-risk (HR)-cervix in IC/IS, patients were divided into two groups: IC/IS and IC/IS + HR-cervix. The differences in dosimetric parameters were compared between the two groups. Comparisons were then made between the parameters of the four planning methods: IC (Point A), IC (three dimensional [3D]), IC/IS, and IC/IS + HR-cervix. RESULTS: In patients with IC/IS implants, the relative uterine tandem dwell time was significantly extended in the IC/IS + HR-cervix group, and the V150 and V200 volumes of HR-cervix were increased (all p < 0.001), whereas the D90 and D100 values of the IC/IS + HR-cervix group were lower than those in the IC/IS group. In pairwise comparisons, HR-cervix V150 and V200 values were lowest in the IC/IS group, followed by the IC (3D), IC/IS + HR-cervix, and IC (Point A) groups. All differences were statistically significant (p < 0.05), with the exception of IC/IS vs. IC (3D). CONCLUSIONS: When treating locally advanced cervical cancer (stage IIB, local residual volume ≥5 cm after external radiotherapy), the IC/IS + HR-cervix optimization method can meet the HR clinical target volume D90 dose requirement, normal tissue dose limits, and can escalate doses to local areas of the cervix.

19.
Zhongguo Zhong Yao Za Zhi ; 44(18): 3954-3959, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31872730

RESUMO

Through investigation,it was found that the main disease of leaves was grey mold on Dendrobium officinale in Hubei province,which has a great impact on the yield and quality of D. officinale. The identification of morphological and molecular biological was used to prove that the pathogen was Botrytis cinerea. Through test the effect of 5 plant source fungicides and 4 antibiotic fungicides on mycelial growth of strain HS1,which proved 0. 3% eugenol had the best inhibitory effect,EC50 was 0. 29 mg·L-1,the second was1% osthol and EC50 was 1. 12 mg·L-1,the EC50 of 0. 5% matrine was 9. 16 mg·L-1,the EC50 of the other six fungicides was higher than 10 mg·L-1. The field control effect test proved that 0. 3% eugenol had the best control effect,reaching 89. 44%,secondly for 1%osthole,which was 77. 17%,0. 5% matrine was in the third place with 62. 37% of effective rate. However,the control effect of the other fungicides was less than 60%. The three plant-derived fungicides were safe for the produce of D. officinale and showed no phytotoxicity. The effect of these fungicides on the growth of D. candidum was tested,and proved that all the fungicides were safe and harmless to D. candidum. This study provides a research basis for the safe and effective prevention and control gray mold of D. officinale.


Assuntos
Botrytis/patogenicidade , Dendrobium/microbiologia , Fungicidas Industriais , Doenças das Plantas/microbiologia , Alcaloides , Botrytis/efeitos dos fármacos , Cumarínicos , Eugenol , Doenças das Plantas/prevenção & controle , Folhas de Planta/microbiologia , Quinolizinas
20.
Mar Environ Res ; 152: 104823, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31676169

RESUMO

The oil spill accidents may drastically impact the environment and ecosystem at intertidal zones. The spilled oil will penetrate the sediments and accumulate to cause lethal or sublethal effects on the benthic invertebrates. An M-BACI experiment was manipulated in situ to assess the ecological responses of benthic macrofauna to different degrees of diesel oil spill. Both biotic and abiotic parameters were studied for 126 days, subjected to both "pulse" and "press" oil contaminations. The content of aliphatic hydrocarbons (displayed as ratios of n-C17/Pr and n-C18/Ph) slightly dropped then continuously existed in the sediment during the experiment time. The macrofaunal assemblage structures were dramatically altered in species number, abundance and biomass. In general, it takes longer time for the macrofauna assemblages to recover under high concentration oil spill than that under low concentration. Our results highlight the diversified strategies for survival and recolonization among dominant species, which distinguish themselves between: i) tolerant species, ii) opportunistic species, and iii) equilibrium species.

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