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1.
Chem Biodivers ; : e2100520, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34585845

RESUMO

Gut dysbiosis contributes to hepatic fibrosis. Emerging evidence revealed the major role of traditional Chinese medicine (TCM) in gut microbiota homeostasis. Here, we aimed to investigate the anti-fibrotic activity and underlying mechanism of ganshuang granules (GS), particularly regarding gut microbiota homeostasis. CCl4 -induced hepatic fibrosis models were allocated into 4 groups receiving normal saline (model), 1.0, 2.0, or 4.0 g/kg GS for 5 weeks. As result, GS treatment alleviated liver injury in CCl4 -induced hepatic fibrosis, presenting as decreases of the liver index, alanine aminotransferase, and aspartate transaminase. Histological staining and expression revealed that the enhanced oxidative stress, inflammatory and hepatic fibrosis in CCl4 -induced models were attenuated by GS. Immunohistochemical staining showed that tight junction-associated proteins in intestinal mucosa were up-regulated by GS. 16S rRNA sequencing showed that GS rebalanced the gut dysbiosis manifested as improving alpha and beta diversity of gut microbiota, reducing the ratio of Firmicutes to Bacteroidetes, and regulating the relative abundance of various bacteria. In summary, GS decreased the intestinal permeability and rebalanced the gut microbiota to reduce the oxidative stress and inflammation, eventually attenuating CCl4 -induced hepatic fibrosis.

2.
J Biomed Nanotechnol ; 17(7): 1284-1292, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34446132

RESUMO

This study aimed to introduce nano-gold PCR for detection of TERT methylation, and explore the correlation between TERT methylation and prognosis of hepatocellular carcinoma (HCC). From March 2016 to March 2018, 154 HBV carriers treated in our hospital were enrolled in the study and divided into HCC (68 cases), cirrhosis (45 cases) and chronic hepatitis (CH) groups (41 cases) based on clinical disease. HCC patients were further divided into methylation (30 cases) and non-methylation (38 cases) subgroup based on methylation status of the TERT. TERT methylation of HCC specimens were 44.12% and 35.24% by nano-PCR and conventional PCR, respectively. The TERT methylation and TERT expression in HCC specimens were higher than for cirrhosis and CH specimens. A significant positive correlation was observed between TERT methylation and TERT expression. AFP, Edmondson classification, tumor size, hilar lymph node and intrahepatic metastasis, and TNM staging in the methylation group were higher than in non-methylation group. Further, overall survival and progression-free survival were significantly shorter. Nano-gold PCR is more sensitive in detecting TERT methylation. As CHB progresses, TERT methylation increases. Greater methylation of the gene is associated with worse prognosis in HCC patients.


Assuntos
Carcinoma Hepatocelular , Hepatite B Crônica , Hepatite B , Neoplasias Hepáticas , Telomerase , Carcinoma Hepatocelular/genética , Metilação de DNA , Ouro , Vírus da Hepatite B/genética , Hepatite B Crônica/genética , Humanos , Neoplasias Hepáticas/genética , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , Telomerase/genética
3.
J Chromatogr A ; 1653: 462428, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34329956

RESUMO

Three novel hypercrosslinked polymers (HCPs) were synthesized via Friedel-Crafts reaction employing 1,3,5-tris(bromomethyl)-2,4,6-trimethylbenzene as alkylating agent, and triphenylbenzene, tetraphenylethylene and p-quaterphenyl as the aromatic units, respectively. The prepared HCPs were applied as solid-phase microextraction coatings for direct immersion extraction of polycyclic aromatic hydrocarbons (PAHs) and their oxygenated and nitrated derivatives in environmental water samples. The key factors affecting the extraction efficiency including extraction time, extraction temperature, stirring rate, ionic strength and desorption conditions, were carefully studied. Coupled with gas chromatography mass spectrometry analysis, a new method for determining PAHs and their derivatives was developed. Under the optimized conditions, the limits of detection (S/N=3) and limits of quantitation (the lowest concentration for quantification) of the method were in the range of 2.5-25.0 and 7.5-75.0 ng L-1, respectively. The recoveries of spiked samples were in the range of 73.1-118.3% with relative standard deviations less than 13.0%. The developed method was applied for the simultaneous determination of nine PAHs and their derivatives in environmental water samples, showing good accuracy and reliability.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas , Hidrocarbonetos Policíclicos Aromáticos , Microextração em Fase Sólida , Poluentes Químicos da Água , Limite de Detecção , Hidrocarbonetos Policíclicos Aromáticos/análise , Polímeros , Reprodutibilidade dos Testes , Poluentes Químicos da Água/análise
4.
J Chromatogr A ; 1651: 462301, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34107399

RESUMO

In this work, a core-shell structured magnetic covalent organic framework named as M-TpDAB was constructed with 3,3'-diaminobenzidine (DAB) and 1,3,5-triformylphloroglucinol (Tp) as building units. M-TpDAB was characterized by infrared spectroscopy, nitrogen adsorption-desorption isotherms, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy. Using the M-TpDAB as adsorbent, a simple and highly effective method was proposed for preconcentrating phenylurea herbicides before high performance liquid-phase chromatography analysis. In the optimized conditions, a good linearity was achieved within the range of 0.15-100 ng mL-1 for water sample, 1.0-100.0 ng mL-1 for tea drink samples. The limits of detection for the analytes were 0.05-0.15 ng mL-1 for water sample and 0.30-0.50 ng mL-1 for drink samples. Satisfactory recoveries of spiked target compounds were in the range of 84.6%-105% for water sample and 80.3%-102% for tea drink samples. Finally, the M-TpDAB based method was successfully used to determine phenylurea herbicides in tea drinks and water samples, demonstrating a good alternative for analyzing trace level of phenylurea herbicides in water samples.


Assuntos
Herbicidas/isolamento & purificação , Estruturas Metalorgânicas/química , Compostos de Fenilureia/isolamento & purificação , Extração em Fase Sólida/métodos , Água/química , Adsorção , Cromatografia Líquida de Alta Pressão , Água Potável/química , Herbicidas/análise , Fenômenos Magnéticos , Magnetismo/métodos , Compostos de Fenilureia/análise , Difração de Raios X
5.
Chin J Physiol ; 64(1): 24-31, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33642341

RESUMO

Accumulating evidence has already indicated that traditional Chinese medicine (TCM) possesses tremendous potential for treating neurodegenerative diseases. Astragalus, also named Huangqi, is a famous traditional medical herb that can be applied to treat cerebral ischemia and prevent neuronal degeneration. Nevertheless, the underlying mechanisms remain largely unexplored. In the present study, Astragalus-containing serum (ASMES) was prepared and added into the culture medium of PC12 cells to explore its neuroprotective effect on 6-hydroxydopamine (6-OHDA)-caused neuronal toxicity. Our data showed that ASMES significantly ameliorated the cellular viability of cultured PC12 cells against the neurotoxicity induced by 6-OHDA (P < 0.05). Moreover, ASMES significantly decreased the cell apoptosis triggered by 6-OHDA (P < 0.01). Furthermore, 2',7'-dichlorofluorescin diacetate assay was performed to detect the changes in oxidative stress, and we showed that 6-OHDA elevated the production of reactive oxygen species (ROS), whereas ASMES significantly reversed these changes (P < 0.01). Besides, mitochondrial membrane potential (MMP) assay showed that ASMES could restore 6-OHDA-damaged MMP in cultured PC12 cells (P < 0.05). In conclusion, Astragalus could protect PC12 cells from 6-OHDA-caused neuronal toxicity, and possibly, the ROS-mediated apoptotic pathway participated in this process. Collectively, our findings provided valuable insights into the potential in treatment of neurodegenerative diseases.


Assuntos
Fármacos Neuroprotetores , Animais , Apoptose , Sobrevivência Celular , Potencial da Membrana Mitocondrial , Fármacos Neuroprotetores/farmacologia , Oxidopamina/toxicidade , Células PC12 , Ratos , Espécies Reativas de Oxigênio
6.
J Chromatogr A ; 1641: 461972, 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33611110

RESUMO

Design and synthesis of novel coatings for solid phase microextraction (SPME) is urgently needed for sample pretreatment. In this study, three hypercrosslinked polymers (HCPs) were constructed by the facile Friedel-Crafts alkylation reactions between tetraphenylethylene (TPE) and 1,4-bis(chloromethyl)benzene (BCMB), 4,4'-bis(chloromethyl)-1,1'-biphenyl (BCMBP), and cyanuric chloride (CC), respectively. The newly-synthesized HCPs were employed as SPME coatings for the extraction of phthalate esters (PAEs). Various parameters influencing the SPME efficiencies, including extraction time and temperature, ionic strength, stirring rate, desorption temperature and time were optimized. Under the optimal conditions, low limits of detection (0.003-0.033 µg L - 1), wide linearity (0.01-10 µg L - 1) and good repeatability (4.1-9.3%) were achieved. The HCPs-based SPME method was successfully applied for the determination of eight PAEs in environmental water and bottled water samples with recoveries from 75.3% to 116%. This method provides a good alternative for monitoring trace level of PAEs in water samples.


Assuntos
Reagentes para Ligações Cruzadas/química , Ésteres/análise , Ácidos Ftálicos/análise , Polímeros/química , Microextração em Fase Sólida/métodos , Poluentes Químicos da Água/análise , Água/química , Água Potável/química , Limite de Detecção , Nanosferas/ultraestrutura , Sais/química , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Fatores de Tempo
7.
Artigo em Inglês | MEDLINE | ID: mdl-33144872

RESUMO

Kang-Xian (KX) pills have been clinically used for the treatment of chronic hepatic injury (CHI). However, the mechanisms of KX on CHI remain unknown. The aim of this study mainly focused on the anti-inflammatory effects of KX in a CHI mouse model based on modulating gut microbiota and gut permeability. We first established a CHI model using carbon tetrachloride (CCl4) and treated it with KX. The anti-inflammatory effects of KX on CHI model mice and the changes in gut permeability after KX treatment were also investigated. 16S rRNA analysis was used to study the changes of gut microbiota composition after KX treatment. In addition, gut microbiota was depleted using a combination of antibiotics in order to further confirm that KX could inhibit the inflammatory response and decrease gut permeability to treat CHI by modulating the gut microbiota. Results showed that KX treatment significantly improved liver function in CHI model mice. KX could also increase the levels of tight junction proteins in the colon and decrease the expression of proinflammatory cytokines in the liver. 16S rRNA analysis indicated that KX treatment affected the alpha and beta diversities in CHI model mice. Further analysis of 16S rRNA sequencing indicated that KX treatment increased the ratio of Firmicutes to Bacteroidetes at the phylum level. At the genus level, KX treatment increased the relative abundance of Lactobacillus, Bacteroides, and Akkermansia and decreased the relative abundance of Ralstonia, Alloprevotella, and Lachnoclostridium. However, KX could not alleviate CHI after depleting the gut microbiota. The effects of KX on gut permeability and inflammatory response in the liver were also decreased following the depletion of gut microbiota. In conclusion, our current study demonstrated that gut microbiota was significantly affected during CHI progression. KX could inhibit the inflammatory response and decrease the gut permeability in CHI model mice through modulating the gut microbiota.

9.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(8): 988-993, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32912415

RESUMO

OBJECTIVE: To establish a predictive model and investigate its value in evaluating short-term prognosis of patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF). METHODS: Patients with HBV-ACLF admitted to Tianjin Second People's Hospital and Beijing Youan Hospital, Capital Medical University from May 2015 to October 2018 were enrolled. The data of gender, age, laboratory markers at admission, model for end-stage liver disease (MELD) score and clinical complications were collected for analysis. According to the prognosis on 12-week, patients were divided into survival group and death group. Univariate analysis and binary Logistic regression analysis were used to test the risk factors for short-term prognosis of the patients with HBV-ACLF, and a prediction model was established. The accuracy of each index and the established model were verified by the receiver operating characteristic (ROC) curve. RESULTS: A total of 148 patients with HBV-ACLF were enrolled in the study, 91 cases survived while 57 cases died during the 12-week period. The age, total bilirubin (TBIL), neutrophil percentage (NEUT%), hepatitis B surface antigen (HBsAg), MELD score of death group were higher than those of survival group [age (years old): 50.00 (44.50, 55.00) vs. 43.00 (34.00, 53.00), TBIL (µmol/L): 310.30 (240.70, 405.70) vs. 266.40 (184.20, 360.20), NEUT%: (74.52±13.05)% vs. (66.64±12.35)%, lgHBsAg (kU/L): 3.72 (3.29, 3.92) vs. 2.97 (2.49, 3.78), MELD score: 24.27 (19.71, 27.40) vs. 21.88 (18.83, 24.38), all P < 0.05], while albumin (ALB), total cholesterol (CHO), prothrombin activity (PTA) and alpha-fetoprotein (AFP) were lower than those of survival group [ALB (g/L): 29.80 (27.05, 31.05) vs. 30.80 (28.00, 33.90), CHO (mmol/L): 1.98 (1.50, 2.38) vs. 2.49 (2.05, 3.01), PTA: (30.37±7.09)% vs. (32.94±6.03)%, AFP (µg/L): 21.54 (9.28, 51.54) vs. 66.16 (24.50, 152.80), all P < 0.05]. Logistic regression analysis showed that NEUT%, HBsAg and AFP were independent risk factors for short-term prognosis of patients with HBV-ACLF [odds ratio (OR) was 77.843, 1.439, 0.995, respectively, all P < 0.05]. According to the results of regression analysis, the NHA-ACLF model (NEUT%+HBsAg+AFP) was established. The formula was logit (NHA-ACLF) = -5.441+5.688×NEUT%+0.430×lgHBsAg-0.005×AFP. The area under the ROC curve (AUC) of the NHA-ACLF model for pred HBV-ACLF patients was 0.790, which was better than NEUT% (AUC = 0.696), lgHBsAg (AUC = 0.670), AFP (AUC = 0.703) and MELD score (AUC = 0.640). When the cut-off value of NHA-ACLF model score was 0.459, the sensitivity was 73.7%, and the specificity was 79.1%. CONCLUSIONS: NEUT%, HBsAg and AFP are independent predictive indicator for short-term prognosis in patients with HBV-ACLF. Compared with MELD score, the risk assessment model NHA-ACLF has a greater value in predicting the short-term prognosis of patients with HBV-ACLF.


Assuntos
Insuficiência Hepática Crônica Agudizada , Vírus da Hepatite B , Humanos , Prognóstico , Curva ROC , Fatores de Risco
10.
J Int Med Res ; 48(9): 300060520931288, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32993381

RESUMO

OBJECTIVE: To identify potentially effective bacterial components of gold juice, a traditional Chinese medicine treatment used for fecal microbiota transplantation. METHODS: Fecal samples were collected from five healthy children (two boys and three girls; mean age, 7.52 ± 2.31 years). The children had no history of antibiotic use or intestinal microecological preparation in the preceding 3 months. Fresh fecal samples were collected from children to prepare gold juice in mid-to-late November, in accordance with traditional Chinese medicine methods, then used within 7 days. Finally, 16S rDNA sequence analysis was used to identify potentially effective bacterial components of gold juice. QIIME software was used for comparisons of microbial species among gold juice, diluent, filtrate, and loess samples. RESULTS: Microflora of gold juice exhibited considerable changes following "ancient method" processing. Microbial components significantly differed between gold juice and filtrate samples. The gold juice analyzed in our study consisted of microbes that synthesize carbohydrates and amino acids by degrading substances, whereas the filtrate contained probiotic flora, Bacteroides, and Prevotella 9. CONCLUSIONS: This study of microbial components in gold juice and filtrate provided evidence regarding effective bacterial components in gold juice, which may aid in clinical decisions concerning fecal microbiota transplantation.


Assuntos
Microbioma Gastrointestinal , Ouro , Criança , Pré-Escolar , Transplante de Microbiota Fecal , Fezes , Feminino , Humanos , Masculino , RNA Ribossômico 16S/genética
11.
Mikrochim Acta ; 187(9): 521, 2020 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-32856135

RESUMO

Graphene-carbon nanosphere composite (G@CNS) was prepared via a simple hydrothermal method. The G@CNS nanocomposite was characterized by X-ray diffraction, scanning electron microscope, surface area, and porosity analysis. The G@CNS was applied as a new sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) prior to quantitative determination by high-performance liquid chromatography with ultraviolet detection at 208 nm. Some experimental parameters including desorption conditions, sample pH, sample volume, and loading rate were studied carefully. Under the optimized condition, the method provided good linearity ranging from 0.3 to 100.0 ng mL-1 with low limits of detection of 0.10-0.20 ng mL-1 for grape juice, 0.10-0.30 ng mL-1 for blend fruit juice, and 0.10-0.20 ng mL-1 for water sample. Good method recoveries (80.2-110%) with relative standard deviations less than 7.2% and high enrichment factors (167-293) were achieved. Results demonstrated that this novel G@CNS can serve as a promising alternative sorbent for more applications. In this work, a graphene-carbon nanosphere (G@CNS) composite was synthesized via a simple hydrothermal method. Then, the G@CNS was served as a novel sorbent for solid-phase extraction of five carbamate pesticides (tsumacide, carbaryl, isoprocarb, bassa, diethofencarb) in juice and environmental water samples, followed by their quantitative analysis with high-performance liquid chromatography-ultraviolet detection.

12.
Sci Total Environ ; 733: 138869, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32450376

RESUMO

Training samples is fundamental for crop mapping from remotely sensed images, but difficult to acquire in many regions through ground survey, causing significant challenge for crop mapping in these regions. In this paper, a transfer learning (TL) workflow is proposed to use the classification model trained in contiguous U.S.A. (CONUS) to identify crop types in other regions. The workflow is based on fact that same crop growing in different regions of world has similar temporal growth pattern. This study selected high confidence pixels across CONUS in the Cropland Data Layer (CDL) and corresponding 30-m 15-day composited NDVI time series generated from harmonized Landat-8 and Sentinel-2 (HLS) data as training samples, trained the Random Forest (RF) classification models and then applied the models to identify crop types in three test regions, namely Hengshui in China (HS), Alberta in Canada (AB), and Nebraska in USA (NE). NDVI time series with different length were used to identify crops, the effect of time-series length on classification accuracies were then evaluated. Furthermore, local training samples in the three test regions were collected and used to identify crops (LO) for comparison. Results showed that overall classification accuracies in HS, AB and NE were 97.79%, 86.45% and 94.86%, respectively, when using TL with NDVI time series of the entire growing season for classification. However, LO could achieve higher classification accuracies earlier than TL. Because the training samples were collected across USA containing multiple growth conditions, it increased the potential that the crop growth environment in test regions could be similar to those of the training samples; but also led to situation that different crops had similar NDVI time series, which caused lower TL classification accuracy in HS at early-season. Generally, this study provides new options for crop classification in regions of training samples shortage.

13.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(12): 1496-1501, 2020 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-33541504

RESUMO

OBJECTIVE: To verify the accuracy of the model for end-stage liver disease-sodium (MELD-Na), chronic liver failure consortium organ failure score (CLIF-C OFs), Chinese Group on the Study of Severe Hepatitis B-Acute-on-chronic Liver Failure score (COSSH-ACLFs) and neutrophil-to-lymphocyte ratio (NLR) scoring systems in patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) and to explore its value in clinical application. METHODS: The clinical data (gender, age, disease stage) and laboratory indicators [alanine transferase (ALT), glutamate transaminase (AST), total bilirubin (TBil), albumin (ALB), blood urea nitrogen (BUN), creatinine (Cr), serum sodium (Na), prothrombin activity (PTA), international standardized ratio (INR), neutrophils count (NEU) and lymphocytes count (LYM)] of 163 patients with HBV-ACLF from July 2010 to July 2018 in Tianjin Second People's Hospital were retrospectively analyzed. After 8 weeks of admission, the patients were divided into death group (90 cases) and survival group (73 cases) according to survival status. The MELD-Na, CLIF-C OFs, COSSH-ACLFs scores and NLR of death group and survival group were compared, and a multivariate Logistic regression analysis was used to analyze the independent risk factors for HBV-ACLF. Propensity score analysis was used to demonstrate the accuracy of the method and receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of the independent risk factors. RESULTS: There were no significant differences in gender, disease stage, ALB, BUN, Cr, Na, NEU on admission between the two groups (all P > 0.05). The age [years old: 43.00 (34.00, 53.00) vs. 50.00 (42.50, 55.00)] and serum levels of ALT [U/L: 252.90 (61.43, 613.33) vs. 359.10 (115.15, 784.70)], AST [U/L: 146.15 (90.88, 449.30) vs. 237.80 (109.00, 635.05)], TBil [µmol/L: 265.10 (183.10, 347.60) vs. 307.50 (229.90, 405.55)] and INR [2.13 (1.91, 2.46) vs. 2.29 (2.02, 2.94)] in survival group were lower than those in death group and the PTA [%: 34.00 (28.00, 38.00) vs. 31.00 (24.00, 36.00)] and LYM [×109/L: 1.37 (0.72, 1.79) vs. 0.85 (0.51, 1.39)] levels were significantly higher than those in death group (both P < 0.05). The MELD-Na [17.99 (16.60, 19.63) vs. 19.16 (17.43, 20.80)], CLIF-C OFs [9.00 (8.00, 9.00) vs. 9.00 (9.00, 10.00)], COSSH-ACLFs [4.87 (4.63, 5.48) vs. 5.47 (5.07, 5.80)] and NLR [2.86 (2.21, 5.19) vs. 4.38 (2.54, 8.46)] were lower in survival group than those of the death group (all P < 0.05). Logistic regression analysis showed that CLIF-C OFs [odds ratio (OR) = 0.532, 95% confidence interval (95%CI) was 0.380-0.744, P < 0.05] and NLR (OR = 0.901, 95%CI was 0.835-0.972, P < 0.05) were the independent risk factors for the prognosis of HBV-ACLF. After propensity score matching, the data of 59 cases in each group were successfully matched, there were no significant differences in age, gender, disease stage, ALT, AST, TBil, ALB, BUN, Cr, Na, PTA, INR and NEU between the two groups (all P > 0.05), and statistically significant difference in the baseline LYM [×109/L: 1.35 (0.74, 1.73) vs. 0.81 (0.51, 1.30)] were found between the survival group and the death group. The CLIF-C OFs, COSSH-ACLFs scores and NLR were lower in survival group compared with those of the death group [CLIF-C OFs: 9.00 (8.00, 9.00) vs. 9.00 (8.00, 10.00), COSSH-ACLFs: 4.99 (4.69, 5.64) vs. 5.34 (5.03, 5.81), NLR: 2.85 (2.21, 5.72) vs. 4.38 (2.47, 10.20), all P < 0.05] and CLIF-C OFs (OR = 0.593, 95%CI was 0.401-0.878, P < 0.05) and NLR (OR = 0.593, 95%CI was 0.401-0.878, P < 0.05) were still as the independent risk factors for the prognosis of HBV-ACLF. The sensitivity of CLIF-C OFs ≥ 9 and NLR ≥ 3.14 to forecast the 8-week clinical outcome of HBV-ACLF patients were 76.7% and 67.1%, the specificity were 48.9% and 56.7%, and AUC were 0.662 and 0.623. CLIF-C OFs was combined with NLR to increase the specificity of forecasting the 8-week clinical outcome of HBV-ACLF patients to 77.8%. CONCLUSIONS: CLIF-C OFs and NLR scores are independent risk factors affecting the clinical outcome of HBV-ACLF, and have better clinical value in predicting the prognosis of HBV-ACLF. Combined application of the two scores will be more beneficial to the prognosis of HBV-ACLF.


Assuntos
Insuficiência Hepática Crônica Agudizada , Doença Hepática Terminal , Insuficiência Hepática Crônica Agudizada/diagnóstico , Vírus da Hepatite B , Humanos , Linfócitos , Neutrófilos , Prognóstico , Curva ROC , Estudos Retrospectivos , Índice de Gravidade de Doença , Sódio
14.
Int J Nanomedicine ; 14: 8989-9006, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819414

RESUMO

Objective: The objective of this study was to develop magnetic embolic microspheres that could be visualized by clinical magnetic resonance imaging (MRI) scanners aiming to improve the efficiency and safety of embolotherapy. Methods and discussion: Magnetic ferrite nanoclusters (FNs) were synthesized with microwave-assisted solvothermal method, and their morphology, particle size, crystalline structure, magnetic properties as well as T2 relaxivity were characterized to confirm the feasibility of FNs as an MRI probe. Magnetic polymer microspheres (FNMs) were then produced by inverse suspension polymerization with FNs embedded inside. The physicochemical and mechanical properties (including morphology, particle size, infrared spectra, elasticity, etc.) of FNMs were investigated, and the magnetic properties and MRI detectable properties of FNMs were also assayed by vibrating sample magnetometer and MRI scanners. Favorable biocompatibility and long-term MRI detectability of FNMs were then studied in mice by subcutaneous injection. FNMs were further used to embolize rabbits' kidneys to evaluate the embolic property and detectability by MRI. Conclusion: FNMs could serve as a promising MRI-visualized embolic material for embolotherapy in the future.


Assuntos
Embolização Terapêutica , Compostos Férricos/química , Imageamento por Ressonância Magnética , Magnetismo , Microesferas , Nanopartículas/química , Polímeros/química , Animais , Difusão Dinâmica da Luz , Elasticidade , Feminino , Ferro/metabolismo , Rim/diagnóstico por imagem , Rim/patologia , Masculino , Camundongos , Nanopartículas/ultraestrutura , Tamanho da Partícula , Espectroscopia Fotoeletrônica , Coelhos
15.
Environ Sci Pollut Res Int ; 26(32): 33607-33620, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31587163

RESUMO

This study aimed to improve the pore size, porosity, and hydrophobicity of polyvinylidene fluoride (PVDF) membranes for desalination by vacuum membrane distillation (VMD). New membranes were prepared via etching PVDF/calcium carbonate (CaCO3) composite membranes using hydrochloric acid (HCl), depending on the chemical reaction of CaCO3 and HCl. Etched membranes were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), contact angle (CA), atomic force microscope (AFM), and scanning electron microscopy (SEM). The results showed that CaCO3 of composite membranes was completely reacted by 1.5 mol/L HCl after composite membranes had been etched 90 min. The crystallinity of etched membranes was the same as that of PVDF/CaCO3 composite membranes, and no new functional groups appeared in etched membranes, which indicated that etched membranes had good chemical stability. The surface roughness increased and led to the increase of contact angle, which means the hydrophobicity of etched membranes was enhanced. As a result, the increment of permeation flux had been improved in a VMD process. It was found that the maximum flux of etched membrane was enhanced and up to 1.65 times of composite membrane when the concentration of sodium chloride (NaCl) solution was 5.0 wt%, and the maximum flux reached up to 30.9 kg m-2 h-1.


Assuntos
Ácido Clorídrico/química , Membranas Artificiais , Polivinil/química , Carbonato de Cálcio/química , Destilação , Interações Hidrofóbicas e Hidrofílicas , Microscopia Eletrônica de Varredura , Porosidade , Cloreto de Sódio , Espectroscopia de Infravermelho com Transformada de Fourier
16.
Int J Phytoremediation ; 21(14): 1407-1414, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31272200

RESUMO

The heavy metals and deleterious element (Pd, Cd, Cu, As, and Hg) in Chinese peony (Paeonia lactiflora Pall.) were determined by Tessier's sequence extraction method. Pb mainly existed in carbonate fraction. The main fraction of Cd in different tissues and different month is quite different. Cu mainly exists as exchangeable carbonate fractions. Five forms of Hg all exist in leaf, stem, and root. The total absorbable fraction of Pd, Cd, Cu, As, and Hg was different in different tissues. The total content of heavy metals can migrate from different tissues and the content of different speciation of heavy metal also can change during the growing period of plants. The results showed that different parts of plants and different elements resulted in different distribution and mobility. Base on this, it is more scientific and reasonable to clarify the migration and enrichment and to analyses the speciation of heavy metals during growing period of plant medicine. It is more scientific and reasonable to clarify the migration and enrichment, and to analyses the speciation of heavy metals during growing period of plant medicine.


Assuntos
Metais Pesados , Paeonia , Biodegradação Ambiental , China , Monitoramento Ambiental
17.
Mikrochim Acta ; 186(7): 464, 2019 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-31230150

RESUMO

Graphene oxide was covalently modified with p-phenylenediamine via a diazonium reaction. The resulting material was employed as a sorbent for the solid-phase extraction of six phenylurea herbicides (metoxuron, monuron, chlortoluron, isoproturon, monolinuron, and buturon) from environmental water and lettuce leafs. Some key factors that influence the extraction efficiency were studied, including sample loading rate, sample pH, and desorption conditions. Following desorption with acetonitrile, the analytes were quantified by HPLC with UV detection. Under optimized conditions, response to phenylurea herbicides is linear in the 2.0-100 ng mL-1 concentration range for water samples, and 5.0-100 ng g-1 for leaf lettuces. The limits of detection are 0.10-0.25 ng mL-1 for water samples, and 1.5-2.5 ng g-1 for leaf lettuces. The sorbent was also applied to the preconcentration of organic compounds including nitroimidazoles, chlorophenols, phenylurea insecticides and phthalates. This shows that this sorbent has a large potential for the enrichment of organic pollutants. Graphical abstract A graphene oxide/p-phenylenediamine (GO@PA) composite was prepared via a simple and environmentally-friendly diazonium reaction between p-phenylenediamine diazonium salt and graphene oxide. It was used as the solid phase extraction (SPE) adsorbent to extract phenylurea herbicides with the extraction efficiency higher than that of commercial C18, multi-walled carbon nanotube and polystyrene polymer. The SPE method was combined with HPLC for simultaneous determination of six phenylurea herbicides in environment water and vegetable samples.

18.
Prep Biochem Biotechnol ; 48(10): 946-953, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30388920

RESUMO

The extraction of daidzein and genistein from soybean has been studied and the kinetic modeling was established using four modeling equations. The goodness of fit was evaluated by statistical errors including the standard error of means (SEM), the adjusted correlation coefficient (R2), and chi-square (χ2). The best model was considered to be the So and Macdonald model and it could give the most adequate description of solid-liquid extraction of daidzein and genistein from soybean sample. The effect of process parameters on extraction yields of daidzein and genistein also has been investigated. The optimized extraction condition was at 333.2 K using 70% ethanol solvent at a solvent-to-solid ratio of 20 mL g-1 with an agitation speed of 300 rpm. The highest extraction yields of daidzein and genistein from soybean were 0.126 ± 0.006 and 0.184 ± 0.013 mg g-1, respectively. The activation energies for extraction kinetics of soybean were found to be 11.10 kJ mol-1 (washing step) and 13.96 kJ mol-1 (diffusion step) for daidzein, 10.47 kJ mol-1 (washing step) and 19.70 kJ mol-1 (diffusion step) for genistein, respectively.


Assuntos
Genisteína/isolamento & purificação , Isoflavonas/isolamento & purificação , Modelos Químicos , Soja/química , Genisteína/química , Isoflavonas/química , Cinética
19.
Front Neurol ; 9: 862, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30459701

RESUMO

Background and objective: Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the best known and the most common monogenic small vessel disease (SVD). Cognitive impairment is an inevitable feature of CADASIL. Total SVD score and global cortical atrophy (GCA) scale were found to be good predictors of poor cognitive performance in community-dwelling adults. We aimed to estimate the association between the total SVD score, GCA scale and the cognitive performance in patients with CADASIL. Methods: We enrolled 20 genetically confirmed CADASIL patients and 20 controls matched by age, gender, and years of education. All participants underwent cognitive assessments to rate the global cognition and individual domain of executive function, information processing speed, memory, language, and visuospatial function. The total SVD score and GCA scale were rated. Results: The CADASIL group performed worse than the controls on all cognition measures. Neither global cognition nor any separate domain of cognition was significantly different among patients grouped by total SVD score. Negative correlations between the GCA score and cognitive performance were observed. Approximately 40% of the variance was explained by the total GCA score in the domains of executive function, information processing speed, and language. The superficial atrophy score was associated with poor performance in most of the domains of cognition. Adding the superficial atrophy score decreased the prediction power of the deep atrophy score on cognitive impairment alone. Conclusions: The GCA score, not the total SVD score, was significantly associated with poor cognitive performance in patients with CADASIL. Adding the superficial atrophy score attenuated the prediction power of the deep atrophy score on cognitive impairment alone.

20.
Molecules ; 23(10)2018 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-30347858

RESUMO

Traditional ionic liquids (ILs) catalysts suffer from the difficulty of product purification and can only be used in homogeneous catalytic systems. In this work, by reacting ILs with co-catalyst (ZnBr2), we successfully converted three polyether imidazole ionic liquids (PIILs), i.e., HO-[Poly-epichlorohydrin-methimidazole]Cl (HO-[PECH-MIM]Cl), HOOC-[Poly-epichlorohydrin-methimidazole]Cl (HOOC-[PECH-MIM]Cl), and H2N-[Poly-epichlorohydrin-methimidazole]Cl (H2N-[PECH-MIM]Cl), to three composite PIIL materials, which were further immobilized on ZSM-5 zeolite by chemical bonding to result in three immobilized catalysts, namely ZSM-5-HO-[PECH-MIM]Cl/[ZnBr2], ZSM-5-HOOC-[PECH-MIM]Cl/[ZnBr2], and ZSM-5-H2N-[PECH-MIM]Cl/[ZnBr2]. Their structures, thermal stabilities, and morphologies were fully characterized by Fourier-transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The amount of composite PIIL immobilized on ZSM-5 was determined by elemental analysis. Catalytic performance of the immobilized catalysts was evaluated through the catalytic synthesis of propylene carbonate (PC) from CO2 and propylene oxide (PO). Influences of reaction temperature, time, and pressure on catalytic performance were investigated through the orthogonal test, and the effect of catalyst circulation was also studied. Under an optimal reaction condition (130 °C, 2.5 MPa, 0.75 h), the composite catalyst, ZSM-5-HOOC- [PECH-MIM]Cl/[ZnBr2], exhibited the best catalytic activity with a conversion rate of 98.3% and selectivity of 97.4%. Significantly, the immobilized catalyst could still maintain high heterogeneous catalytic activity even after being reused for eight cycles.


Assuntos
Imidazóis/química , Líquidos Iônicos/química , Propano/análogos & derivados , Zeolitas/química , Alcenos/química , Apatitas/química , Dióxido de Carbono/química , Catálise , Imidazóis/síntese química , Líquidos Iônicos/síntese química , Microscopia Eletrônica de Varredura , Propano/síntese química , Propano/química , Difração de Raios X
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