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1.
J Int Med Res ; 49(4): 3000605211004769, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33823631

RESUMO

OBJECTIVE: To explore the clinical importance of the distribution of pulmonary artery embolism in acute pulmonary embolism (APE). METHODS: Sixty-four patients with APE were classified into mixed-type and distal-type pulmonary embolism groups. Their right ventricular systolic pressure (RVSP) and disease duration were recorded, and the diameter of their right ventricles was measured by ultrasound. The computed tomography angiographic clot load was determined as a Mastora score. RESULTS: Patients with distal-type pulmonary embolisms had significantly lower RVSPs (44.92 ± 17.04 vs 55.69 ± 17.66 mmHg), and significantly smaller right ventricular diameters (21.08 ± 3.06 vs 23.37 ± 3.48 mm) than those with mixed-type pulmonary embolisms. Additionally, disease duration was significantly longer in patients with distal-type pulmonary embolisms (14.33 ± 11.57 vs 8.10 ± 7.10 days), and they had significantly lower Mastora scores (20.91% ± 18.92% vs 43.96% ± 18.30%) than patients with mixed-type pulmonary embolisms. After treatment, RVSPs decreased significantly in patients with both distal-type and mixed-type pulmonary embolisms. Right ventricle diameters also decreased significantly in patients with mixed-type pulmonary embolisms after treatment. CONCLUSION: Patients with mixed-type pulmonary embolisms are significantly more susceptible to pulmonary hypertension, enlarged right ventricular diameters, and shorter durations of disease than those with distal-type pulmonary embolisms. The distribution of pulmonary artery embolism in APE can provide a clinical reference.


Assuntos
Hipertensão Pulmonar , Embolia Pulmonar , Doença Aguda , Angiografia , Feminino , Humanos , Hipertensão Pulmonar/complicações , Hipertensão Pulmonar/diagnóstico por imagem , Masculino , Artéria Pulmonar/diagnóstico por imagem , Embolia Pulmonar/complicações , Embolia Pulmonar/diagnóstico por imagem
2.
Gynecol Endocrinol ; : 1-5, 2021 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-33870841

RESUMO

OBJECTIVE: To summarize the clinical characteristics of Turner syndrome (TS) with a small supernumerary marker chromosome (sSMC) and discuss the clinical significance and management of TS patients with sSMC. METHODS: A retrospective analysis was conducted on the clinical data of 244 patients with disorders of sexual development admitted to Peking Union Medical College Hospital from February 1984 to July 2020. RESULTS: Among the 244 patients with a disorder of sexual development, 69 cases of TS were identified in which 13 patients had sSMC. Their ages ranged from 3 to 28 years old with an average of 14.31 ± 6.40 years. All 13 sSMC-positive patients had typical clinical manifestations of TS except ambiguous genitalia in four cases. SRY gene testing was performed in 11sSMC-positive patients and 10 patients were positive for SRY and one was negative. Among the 10 SRY-positive patients, two cases had hirsutism and clitoral enlargement and two cases had clitoral enlargement only. Nine sSMC and SRY-positive patients underwent gonadectomy and one had left gonadal gonadoblastoma with seminoma in situ and right gonadal seminoma in situ. CONCLUSIONS: Although the sSMC positive detection rate in DSD patients is uncommon (5.33% in our sample), the positive SRY detection rate in sSMC-positive TS patients was extremely high in our TS patients. And TS patients with sSMC and SRY positive had a significantly increased risk of gonadal germ cell tumors. Routine SRY screening should be performed in TS patients with sSMC, and a gonadectomy should be performed in TS patients with sSMC and SRY positive to prevent the occurrence of tumors.

3.
Stem Cell Res ; 53: 102292, 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33774333

RESUMO

Mental retardation, X-linked 21/34 (MRX21/34), is a rare intellectual disability disease caused by mutations in the IL1RAPL1 (Interleukin-1 Receptor Accessory Protein-Like 1) gene. Using Sendai virus-mediated reprogramming, we established an induced pluripotent stem cell (iPSC) line from PBMCs collected from a ten-year-old boy with MRX21/34. The iPSCs showed stable amplification, expressed pluripotent genes, displayed a normal karyotype, and had characteristics of trilineage differentiation potential in an in vitro differentiation assay.

4.
Environ Pollut ; 273: 116435, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33460867

RESUMO

Organic fraction municipal solid waste (OFMSW) has a high potential for energy and value-added product recovery due to its carbon- and nutrient-rich composition; however, traditional value chains have treated OFMSW as an undesired by-product. This study focuses on value chain optimisation to assist the transition to resource recovery value chains. To achieve this, this work combined two stage stochastic mathematical optimisation with geographical spatial analysis and time series waste generation analysis. Existing infrastructure in England, including anaerobic digestion plants and road transportation networks, were included in the model. To account for uncertainty in waste generation, multiple scenarios and their associated probabilities were developed based on environmental variables. The optimisation problem was solved to further advance the understanding of economically optimal waste-to-resource value chains under waste generation variability. The pertinent decision variables included sizing, technology selection, waste flows and location of thermochemical treatment sites. The model highlights the potential reduction in system profitability as a result of different operating constraints, such as minimum plant operating capacity factors and landfill taxation. The latter was shown to have the largest impact on profitability as overconservative systems designs were implemented to hedge against the waste variability. Such computer-aided models offer opportunities to overcome the challenges posed by waste generation variability and waste to resource value chain transformation.

5.
J Environ Manage ; 280: 111655, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33309109

RESUMO

Rural toilet retrofitting (RTR) is a complex, dynamic system that is affected by many factors and the positive/negative feedback relationships between subsystems and variables. Traditional technologies and management methods face challenges in fundamentally describing and solving problems in RTR. To bridge this gap, this study utilizes system dynamics and causal loop diagrams to explain such problems based on data collected from the stakeholders of the RTR in Jiaozhou from 2018 to 2019. Specifically, this study examines the RTR system from the perspectives of household users, wastewater treatment plants, local governments, grassroots promoters, operation and maintenance personnel, toilet supplier and construction teams, and fecal sludge end users. The factors and processes involved in RTR are identified, and the feedback and relationships among its major stakeholders are established. Results show that the motivation of farmers to engage in RTR is a key variable that affects their final decisions regarding retrofitting and maintaining toilet functions. Meanwhile, the important variables related to the feedback and relationships among the major stakeholders of RTR are mostly focused on policies, subsidies, technology, satisfaction, and cooperation. A scientific analysis method and the updated RTR plan for toilet revolution are then formulated to promote the implementation of RTR in developing countries.


Assuntos
Aparelho Sanitário , China , Humanos , População Rural , Saneamento , Toaletes
6.
iScience ; 23(11): 101743, 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33225248

RESUMO

Urban rooftop functional design offers a promising option to enable multi-function urban land-use to deliver multiple ecosystem services, e.g., food production by rooftop agriculture and energy supply by installing photovoltaic (PV) panels. To identify the best rooftop utilization strategy considering multiple decision criteria and understand the impact of rooftop solution on the design of urban energy systems, we propose a whole system modeling framework that integrates biogeochemical simulation and multi-objective energy system optimization. We apply the framework to evaluate three rooftop agriculture options, namely, basic rooftop farming, unconditioned greenhouse, and conditioned greenhouse, and one rooftop energy supply option, i.e., PV panels, for an urban energy eco-design case in Shanghai, China. Enabling rooftop agriculture options brings more flexibility to the design and operation of energy systems. PV panels provide cost-optimal solutions, whereas conditioned greenhouse potentially delivers environmentally sustainable land-use by contributing to climate regulation ecosystem services.

7.
Curr Drug Metab ; 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33115392

RESUMO

BACKGROUND: The large interindividual variability in the genetic polymorphisms of sirolimus (SIR)-metabolizing enzymes, transporters, and receptors can lead to qualitatively and quantitatively distinct its therapeutic responses. OBJECTIVE: We examined the impact of numerous candidate single-nucleotide polymorphisms (SNPs) involved in the trough concentration of SIR-based immunosuppressant regimen. METHOD: This was a retrospective long-term cohort study involving 69 renal allograft recipients. Total DNA was isolated from recipient blood samples and trough SIR concentrations were measured by microparticle enzyme immunoassay. Genome sequence reading was targeted based on next-generation sequencing. The association of tagger SNPs to SIR trough concentrations with non-genetic covariate adjusting was analyzed using logistic regression. RESULTS: A total of 300 SNPs were genotyped in the recipient DNA samples using target sequencing analysis. Only the SNP of CYP3A4 (Ch7: 99361466 C> T, rs2242480) had a significantly higher association with SIR trough concentration as compared to the other 36 tagger SNPs. The mean trough SIR concentration of patients in the CYP3A4 rs2242480-CC group was more significant compared to that of the CYP3A4 rs2242480-TC and TT group, respectively 533.3; 157.4 and 142.5 (ng/ml)/mg/kg, P<0.0001. After adjusting the SNPs, there was no significant association between clinical factors such as age, follow-up period, incidence of delayed graft function, immunosuppression protocol, and sex with SIR trough concentration. CONCLUSION: These findings indicated the significant association of polymorphism in the CYP3A4 (Ch7: 99361466 C> T, rs2242480) with SIR trough concentration after 1-year administration in patients who have undergone kidney transplantation.

8.
Sci Total Environ ; : 142826, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33109366

RESUMO

The global food system inextricably connects human health and environmental integrity. It holds the transformative capability to significantly reduce levels of environmental degradation, caused by current food production practices, and alleviate the 'triple burden' of malnutrition, existing due to food consumption patterns. System-wide transitions are therefore paramount to tackling environmental and nutritional challenges that are exacerbated by a rapidly growing population. This work presents a novel application of Data Envelopment Analysis (DEA) to study the sustainability of food supply patterns around the world and appraise the potential to lower environmental pressure without compromising the supply of calories and nutritional quality. By relating environmental impacts to caloric availability and nutritional adequacy, DEA computes a relative performance score for 139 countries and identifies only 18 countries with per capita food supplies that are 'efficient' in transforming five environmental inputs (land use, greenhouse gas emissions, acidification potential, eutrophication potential and freshwater withdrawals) into calories and nutrition. The widespread extent of 'inefficiency' stresses that the significant opportunity and need to reduce environmental impacts from food is truly global and extensive. Results of this analysis also provide quantitative information on the varying degrees of potential to improve the ways in which each nation's population is fed and therefore offers country-specific insight for decision-makers into the integration of environmental and nutritional outcomes for sustainable development.

9.
Langmuir ; 36(39): 11528-11537, 2020 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-32883083

RESUMO

Mesoporous silica MCM-48 with rich silanol was prepared using polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) as mixed templates, and the dynamic adsorption performance of acetone was evaluated by testing breakthrough curves. The mixed micelle formed by CTAB and PVP, as well as the hydrogen bond between the carbonyl group of PVP and silanol group affected the condensation process of Si-OH group during the formation of mesoporous structure, resulting in the increase of Si-OH group number on the surface of MCM-48. Compared with MCM-48 synthesized by single template (CTAB), the acetone adsorption capacity of MCM-48 (1:3) synthesized by mixed templates (PVP:CTAB = 1:3) improved by 23.86%, which was attributed to the increase of silanol group amount and the decrease of pore size. In addition, Bangham model had the highest goodness of fit to describe the adsorption process among four kinetic models for the adsorbents, conforming to the mechanism of pore diffusion. The Langmuir and Freundlich models were used to fit the adsorption isotherm data, and the Freundlich model could better describe the adsorption of acetone. Freundlich model fitting results showed that MCM-48 with rich silanol had a strong affinity for acetone, and the adsorption of acetone on MCM-48 belonged to multilayer adsorption. The thermodynamic results showed that the adsorption of MCM-48 for acetone was physical adsorption, and the adsorption behavior was exothermic. This work provided insight into how the inherent properties of an adsorbent and environmental factors (including initial concentration and adsorption temperature) affected the adsorption performance of ketones, thus more ideas could be provided for the accurate design of adsorbents. Furthermore, silanol-rich MCM-48 synthesized by mixed templates is expected to be a promising adsorbent for acetone removal.

10.
Histol Histopathol ; 35(10): 1189-1196, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32975307

RESUMO

CX3CL1 and its receptor CX3CR1 axis are involved in the development, progression and metastasis of many types of cancers. It has been reported that CX3CL1 and CX3CR1 expression was upregulated in some solid tumors. However, their roles in thyroid cancer remain unknown. In the present study, we investigated the expression of CX3CL1 and CX3CR1 in human papillary thyroid carcinoma (PTC) and their clinical significance. In this study, using immunohistochemistry, we examined the expression of CX3CL1 and CX3CR1 in the tissues of 26 human PTC (including 17 classical or conventional (CPTC) and 9 follicular (FVPTC) variants of PTC; 15 cases without and 11 cases with lymph node metastasis) and 10 cases of nodular goiter (NG). Compared to NG, a significant increase in the expression of CX3CL1 and CX3CR1 was found in PTC overall, as well as in CPTC and FVPTC separately. Higher CX3CL1 expression was found in CPTC than in FVPTC, but there was no significant difference in CX3CR1 expression between these subtypes of PTC. When analyzing their expressions in PTC without and with lymph node metastasis, an increased expression of CX3CL1 and CX3CR1 was observed when compared to NG respectively. There was however no significant difference in CX3CL1 and CX3CR1 expressions in PTC without lymph node metastasis when compared to PTC with lymph node metastasis. Furthermore, when compared to NG, an increased expression of CX3CL1 was correlated with an increased expression of CX3CR1 in PTC. Our data indicate that CX3CL1 and CX3CR1 can be used as tumor markers for PTC and may be potential novel targets for cancer prevention and treatment.

11.
Microbiol Res ; 240: 126559, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32721821

RESUMO

Deinococcus radiodurans is able to survive under extreme conditions, including high doses of ionizing radiation, desiccation and oxidative stress. In addition to enhanced DNA repair capabilities, an effective antioxidation system plays an important role in its robustness. Previous studies have linked the radiation resistance of D. radiodurans to its prolonged desiccation tolerance phenotype, which both cause DNA damage. In the current study, we investigated the roles of dr_1172 in D. radiodurans, the gene encoding a typical group 3 LEA protein (DrLEA3) conserved within Deinococcus species. In addition to the increased transcriptional level under oxidative stress, the inactivation of dr_1172-sensitized cells to H2O2 treatments and the reduced cellular antioxidation activities suggested that dr_1172 is involved in the cellular defense against oxidative stress. Moreover, DrLEA3 was enriched at the cell membrane and bound to various types of metal ions. Cells devoid of DrLEA3 showed a decreased intracellular Mn/Fe concentration ratio, indicating that DrLEA3 also plays a role in maintaining metal ion homeostasis in vivo.

12.
Data Brief ; 30: 105593, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32382606

RESUMO

This article presents data affiliated with life cycle inventories, environmental impact and operational sustainability used in, the influence of raw material availability and utility power consumption on the sustainability of the ammonia process [1]. Scenario specific operating conditions were used to simulate the ammonia process based on unique constraints occurring within the Trinidad and Tobago energy sector. The data was collected using AspenⓇ Plus simulations and validated against plant operating data. The data consists of an economic cost evaluation as well as environmental impact using the CML-IA Baseline midpoint approach. The data was derived from life cycle inventories aligned to input/output material and energy flows within the ammonia process as well as life cycle assessment databases utilizing Ecoinvent v3.4. The data can be applied to the wider ammonia supply chain, aiding in achieving greater sustainable development within ammonia-based process industries.

13.
Water Res ; 178: 115842, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32361350

RESUMO

Due to complex composition of carbohydrates, lipid, protein, cellulose, hemicellulose and lignin, wastewater (WW) and organic fraction municipal solid waste (OFMSW) represent nutrient and carbon rich resources. Conventionally, value chains in the waste sector have considered OFMSW and WW as unwanted by-products as opposed to potential valuable resources. Full exploitation of these resources calls for a value chain transformation towards proactive resource recovery. This study focuses on the waste supply chain optimisation to recover value added products from OFMSW. The research leads to a systems-modelling approach, which integrates spatial data analyses, mathematical mixed integer linear programming (MILP) optimisation and technology performance evaluation to inform the design of waste-to-resource value chains. A UK based study on OFMSW is presented to demonstrate the efficacy of the approach. The study captures variation in OFMSW quantity and composition, incorporating over 600 existing anaerobic digestion (AD) operational plants in the UK, while potential sites for new waste-recovery facilities are identified, accounting for transportation and logistics, using a GIS-based analysis. Key outcomes are analysed (technology type, size, location, logistical connections), placing emphasis on the need to consider the value of the resource recovery potential over the lifetime of an AD or thermochemical treatment facility in the design process. Such an approach offers a promising pathway for tackling the open challenges currently hindering the waste-to-resource transformation.


Assuntos
Eliminação de Resíduos , Anaerobiose , Reatores Biológicos , Metano , Resíduos Sólidos
14.
Pharmgenomics Pers Med ; 13: 141-149, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32368128

RESUMO

Background: Tacrolimus (TAC) is beneficial for patients with idiopathic membranous nephropathy (IMN). It has a narrow therapeutic concentration range and many factors influence TAC blood concentration. CYP3A5 is the most important enzyme in TAC metabolism. The aim of this study was to analyze the effects of CYP3A5 gene polymorphisms on the efficacy and safety of TAC in IMN patients. Patients and Methods: Patients with IMN who received oral TAC (0.05-0.075mg/kg/day) combined with prednisone (0.5mg/kg/day) from March 2016 to October 2018 were included. The data of clinical characteristics, therapeutic drugs and adverse reactions of patients were collected at baseline and during 24 weeks of treatment. Patients were divided into two groups according to different CYP3A5 genetic polymorphisms. The significant differences in the efficacy and side effects between the two groups were analyzed. Results: A total of 76 patients who completed follow-up were divided into CYP3A5 nonexpresser (CYP3A5*3/*3) group and CYP3A5 expresser (CYP3A5 *1/*3) group. The significant association between the CYP3A5 phenotype and TAC metabolism was observed. A total of 43 case-times patients exhibited adverse effects. The infection rate in CYP3A5 nonexpresser group (21.95%) was remarkably higher than the rate in CYP3A5 expresser group (5.71%). Blood concentration and C0/D levels were risk factors for adverse events through logistic regression analysis. There was no statistical difference between the study groups with respect to the efficacy. Conclusion: Our results demonstrated that CYP3A5 polymorphisms had important guiding roles in the treatment of IMN with tacrolimus. CYP3A5 expressers required higher daily doses of TAC to achieve the target drug concentration, but with fewer side effects. CYP3A5 genetic polymorphism might be used for TAC dosing adjustment to optimize the treatment for patients with IMN.

15.
Ann Transl Med ; 8(5): 211, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32309358

RESUMO

Background: We aimed to explore the influence of single nucleotide polymorphisms (SNPs) in NFATC1 gene on the occurrence of biopsy-proven acute rejection (BPAR) in renal transplant recipients. Methods: Blood samples from 131 subjects with stable allograft function (STA) and 69 with BPAR episodes were collected and analyzed using target sequencing (TS) with an established panel. Odds ratios (OR) and 95% confidence intervals (95% CIs) were calculated for logistic regression models adjusted for confounding factors. Pathological changes were extracted and the relationship with tagger SNPs was calculated. Moreover, the CCK-8 assay was performed to explore the proliferation of T lymphocytes, and PCR, Western blotting and enzyme-linked immunosorbent assay were applied to identify the effect of mutant on the activation of T cells. Results: High-quality readouts were obtained for 55 NFATC1 SNPs and 14 tagger SNPs were remained for further analysis. After adjusting for clinical confounding factors, the distribution of four NFATC1 SNPs, including rs2290154, rs2304738, rs754093 and rs754096, were statistically significant between STA and BPAR groups. Pathological association analysis indicated one SNP, rs2290154, was significantly related with the Banff score and renal tubulitis. Our in vitro study suggested that NFATC1 rs2290154 mutant could remarkably promote the T cell proliferation, increase the transcription of NFATC1 mRNA and expression of NFATC1 protein, as well as the interleukin-2 (IL-2) secretion. Conclusions: We reported the crucial association of NFATC1 gene with the occurrence of acute rejection (AR) episodes. Moreover, in vitro NFATC1 rs2290154 was significantly involved in the T lymphocytes activation and proliferation through increasing the translation of NFATC1 mRNA and expression of NFATC1 protein, along with the secretion of IL-2.

16.
Anim Reprod Sci ; 214: 106287, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32087914

RESUMO

Estrogen receptor alpha (ERα) is a ligand-activated transcription factor that regulates cellular responses to estrogens and transcription processes of target genes. In this study, changes in DNA methylation and histone modifications in the promoter region and Exon 1 of the ERα gene were analyzed to ascertain epigenetic changes associated with increased ERα mRNA abundance during reproductive maturation from 90 (egg production not yet initiated) to 160 (after egg production was initiated) d of age (d post-hatching) in chicken ovaries. The results indicate there was no difference in CpG methylation at the promoter and Exon 1 except at the region analyzed with primer pairs F2 and R2, where percentage of methylated CpG of Sites 2 and 8 after reproductive maturation was greater compared with before reproductive maturation. By using the chromatin immunuoprecipitation (ChIP) assay combined with SYBR green quantitative PCR, effects of histone modifications were evaluated, including histone H3K4 di + tri methylation, H3K9 phosphorylation and trimethylation, H3K36 methylation and H3K27 acetylation on chicken ERα mRNA transcript abundance. The results indicated that there was a greater histone H3K27 acetylation and lesser H3K36 trimethylation associated with increased abundance of ERα mRNA transcript in chicken ovaries after reproductive maturation (90 compared with 160 d of age). In consistent with this finding, the relative abundance of transcriptional coactivator p300 mRNA transcript and protein in the ovaries was markedly greater in reproductively mature than immature chickens. Findings provide insights into the epigenetic regulations of the chicken ERα gene expression that is required for chicken ovarian development.

17.
Sci Total Environ ; 713: 136359, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32019007

RESUMO

Consumer demand has increased for pastured poultry products as the drive for sustainable farming practices and ethical treatments of livestock have become popular in the press. It is necessary to identify the important meteorological factors associated with the prevalence of Salmonella in the pastured poultry settings since the presence of Salmonella in the environment could lead to contamination of the final product. The objective of this study was to develop a model to describe the relationship between meteorological factors and the presence of Salmonella on the pastured poultry farms. The random forest method was used to develop a model where 83 meteorological factors were included as the predicting variables. The soil model identified humidity as the most important variable associated with Salmonella prevalence, while high wind gust speed and average temperature were identified as important meteorological variables in the feces model. The developed models were robust in predicting the prevalence of Salmonella in pastured poultry farms with the area under receiver operating characteristic (ROC) curve values of 0.884 and 0.872 for the soil model and feces model, respectively. The predictive models developed in this study can provide users with practical and effective tools to make informed decisions with scientific evidence regarding the meteorological parameters that are important to monitor for increased on-farm Salmonella prevalence.


Assuntos
Salmonelose Animal , Salmonella , Animais , Fazendas , Conceitos Meteorológicos , Aves Domésticas , Prevalência , Sudeste dos Estados Unidos
18.
Rev Sci Instrum ; 91(1): 014103, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32012638

RESUMO

PC software-based portable cyclic voltammetry (PCV) systems have the advantages of portability, high performance, and real-time detection. In this paper, the PCV system used cyclic voltammetry (CV) as the main detection and analysis method and contained the following components: a three-electrode unit, a portable potentiostat, and PC software. The PC software was used as the system control and display, and a dynamic peak position adjustment (DPPA) algorithm for E. coli measurements based on thick biofilm modification on electrodes was designed especially for this system to realize the real-time correspondence between the measured results and the modified electrodes. The performance test results obtained by setting different detection parameters in the PCV system were compared with those of commercial electrochemical workstations. The difference was less than 4.99%, with a relative standard deviation less than 0.20%. An electrochemical biosensor based on a Prussian blue-multiwalled carbon nanotube-gold nanoparticle composite was developed for E. coli detection. After constructing an antibody-BSA-E. coli electrode modification on the sensor, experimental data processed by the DPPA algorithm showed that the logarithm (lg DfE.coli) of the E. coli dilution factor and the peak current response had a linear relationship. The PCV system could quickly and accurately detect E. coli concentrations with dynamic adjustment algorithms for biofilm-modified electrodes. Furthermore, the system could detect the electrochemical activities of various high-sensitivity biomolecules, showing great detection potential for on-site monitoring and meeting the requirements of real-time and portable detection in various food safety fields.


Assuntos
Algoritmos , Biofilmes , Técnicas Eletroquímicas , Escherichia coli/fisiologia , Software , Ouro/química , Nanopartículas Metálicas/química , Nanotubos de Carbono/química
19.
Nanotechnology ; 31(16): 165403, 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-31891915

RESUMO

Flexible all-solid-state supercapacitors (ASSSs) have caught the scientific attention to meet the explosive demand for portable and wearable electronic devices. However, it is difficult for flexible electrode materials to obtain a high areal capacitance at a high mass loading, which limits their commercial applications. In this study, vanadium oxide (V2O5) nanoparticles are introduced into Ti3C2 flakes with the aid of cetyltrimethylammonium bromide (CTAB). The intercalation of V2O5 particles in the interlayer of Ti3C2 establishes a hierarchical structure and facilitates the electrolyte penetration. As a result, the prepared CT-Ti3C2@V2O5 composite electrode achieves a high areal capacitance of 2065 mF cm-2 at 3 mA cm-2 and superior active mass loading (15 mg cm-2). Meanwhile, over 93% capacitance is maintained after 6000 cycles at 18 mA cm-2. The ASSS based on CT-Ti3C2@V2O5 delivers a high areal capacitance of 477 mF cm-2 at 1 mV s-1 and exhibits stable performance at different bending states, which reaches to the advanced level for the ASSSs based on MXenes.

20.
Int J Mol Med ; 45(2): 623-633, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894313

RESUMO

The objective of the present study was to investigate the molecular mechanism underlying the role of metformin (Met) in reducing the risk of endometrial hyperplasia (EH). Reverse transcription­quantitative polymerase chain reaction, western blot and immunohistochemistry (IHC) assays were used to study the effects of Met and tamoxifen on the expression levels of urothelial cancer associated 1 (UCA1), microRNA­144 (miR­144) and other factors along the transforming growth factor­ß1 (TGF­ß1)/protein kinase B (AKT) signaling pathway. In addition, MTT and flow cytometry assays were performed to detect the effect of Met on cell proliferation and apoptosis. Tamoxifen treatment increased the weight of the uterus and the level of UCA1, while decreasing the expression of miR­144. In addition, treatment with tamoxifen (2.0 and 3.5 µg) upregulated the protein expression levels of TGF­ß and p­AKT, while downregulating the protein expression of active Caspase­3 in a dose­dependent manner. By contrast, Met reduced cell viability, promoted cell apoptosis, and reduced the expression levels of UCA1, TGF­ß and p­AKT, while upregulating the expression of miR­144 and active Caspase­3 in a dose­dependent manner. Furthermore, Met also reduced the weight of uterus. However, tamoxifen and Met did not exert any effect on the protein levels of total AKT and total Caspase­3. The levels of TGF­ß and p­AKT proteins in the EH group were much higher when compared with those in the sham group, while Met treatment reduced these protein levels to a certain extent. In addition, the expression of active Caspase­3 in the EH group was much lower than that in the sham group, while Met treatment increased its level to a certain extent. In conclusion, the current study suggested that Met reduces the risk of EH by reducing the expression levels of UCA1, TGF­ß and p­AKT, while increasing the levels of miR­144 and active Caspase­3 in a dose­dependent manner.


Assuntos
Hiperplasia Endometrial/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Metformina/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Animais , Antineoplásicos Hormonais/uso terapêutico , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Hiperplasia Endometrial/genética , Hiperplasia Endometrial/metabolismo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Tamoxifeno/uso terapêutico , Fator de Crescimento Transformador beta1/genética , Fator de Crescimento Transformador beta1/metabolismo
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