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1.
Cell Res ; 33(1): 55-70, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36588115

RESUMO

Microphthalmia transcription factor (MITF) regulates melanocyte development and is the "lineage-specific survival" oncogene of melanoma. MITF is essential for melanoma initiation, progression, and relapse and has been considered an important therapeutic target; however, direct inhibition of MITF through small molecules is considered impossible, due to the absence of a ligand-binding pocket for drug design. Here, our structural analyses show that the structure of MITF is hyperdynamic because of its out-of-register leucine zipper with a 3-residue insertion. The dynamic MITF is highly vulnerable to dimer-disrupting mutations, as we observed that MITF loss-of-function mutations in human Waardenburg syndrome type 2 A are frequently located on the dimer interface and disrupt the dimer forming ability accordingly. These observations suggest a unique opportunity to inhibit MITF with small molecules capable of disrupting the MITF dimer. From a high throughput screening against 654,650 compounds, we discovered compound TT-012, which specifically binds to dynamic MITF and destroys the latter's dimer formation and DNA-binding ability. Using chromatin immunoprecipitation assay and RNA sequencing, we showed that TT-012 inhibits the transcriptional activity of MITF in B16F10 melanoma cells. In addition, TT-012 inhibits the growth of high-MITF melanoma cells, and inhibits the tumor growth and metastasis with tolerable toxicity to liver and immune cells in animal models. Together, this study demonstrates a unique hyperdynamic dimer interface in melanoma oncoprotein MITF, and reveals a novel approach to therapeutically suppress MITF activity.


Assuntos
Melanoma , Microftalmia , Animais , Humanos , Fatores de Transcrição/metabolismo , Microftalmia/genética , Melanoma/tratamento farmacológico , Melanoma/metabolismo , Regulação da Expressão Gênica , Proteínas Oncogênicas/genética , Fator de Transcrição Associado à Microftalmia/genética , Fator de Transcrição Associado à Microftalmia/metabolismo , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica
2.
Microbiol Res ; 269: 127313, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36696866

RESUMO

Protein phosphatase 2 A (PP2A) is a major heterotrimeric serine/threonine protein phosphatase comprised of three subunits, including structural subunits (A), regulatory subunits (B), and catalytic subunits (C). PP2A has been widely shown to involve in a series of cell signal transduction processes such as cell metabolism, cell cycle regulation, DNA replication, gene transcription and protein translation in yeast and mammalian. However, the roles of PP2A in pathogenic fungi Magnaporthe oryzae still remain unclear. We here found that MoRts1, a gene encoding B regulatory subunit of PP2A homologous to Saccharomyces cerevisiae Rts1, showed up-regulated transcription during conidia and initially infectious stage. Subcellular localization revealed that MoRts1-eGFP was localized to the cytoplasm and septum. Targeted disruption of MoRts1 leads to a reduction of mycelial growth and sporulation, as well as the defects of hydrophobicity, melanin pigmentation and cell wall integrity (CWI). The MoRts1 mutants were less pathogenic to the host plants, compared to the Ku80 strain, and the transcriptional levels of several pathogenicity-related Rho GTPase genes, including MoCdc42, MoRho2, MoRho3, MoRho4, MoRhoX and MoRac1, were significantly decreased in the MoRts1 mutants. Besides, two splicing variants of MoRts1 with unique functions of regulating the growth and pathogenicity were identified, and the B56 domain is vital for determining the sporulation and pathogenicity of M. oryzae. Furthermore, MoRts1 was identified to interact with PP2A catalytic subunit MoPPG1 in vivo in M. oryzae. In summary, our results showed that MoRts1 is an important regulator contributing to the fungal development, and pathogenicity of M. oryzae.

3.
J Hazard Mater ; 446: 130746, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36630880

RESUMO

The complete detoxification and resource utilization of stainless steel slag are still facing challenges. In this paper, the harmless treatment of industrial stainless steel slag was realized by using a high temperature modification-crystallization control approach, and the influence of binary basicity (B) on selective enrichment of Cr-containing spinel in the slag and corresponding detoxification effect were systematically explored. Results demonstrated that the enrichment degree of Cr and the amount of Cr-bearing spinel separately reached 98.62 wt% and 13.32 wt% when B= 1.2. The reason was that low alkalinity promoted the reaction of Cr in slag to form Cr-containing spinel, thus improving the enrichment of Cr in the spinel and reducing the occurrence probability of Cr in the matrix phase. Furthermore, the toxicity test of modified slag powder was proceeded according to Chinese standard HJ/T 299-2007 and American standard EPA (Toxicity Characteristic Leaching Procedure, TCLP), and the feasibility of using modified slags as admixture for producing cement was discussed. Even if TCLP was adopted, the Cr leaching concentration in modified slag powder with B= 1.2 was only 1.59 mg/L, which was far lower than the national limit of heavy metal leaching concentration (15 mg/L) of solid waste. Remarkably, when the powder was formed as cement, the Cr leaching concentration was even lower than ICP-OES detection line. Meanwhile, its mechanical property was better than that of cement prepared without powder, indicating the detoxified slag powder met the requirements of concrete admixture. This paper provides a new way with certain economic value for detoxification and large-scale utilization of stainless steel slag.

4.
BMC Infect Dis ; 23(1): 35, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36670360

RESUMO

BACKGROUND: Escherichia fergusonii is a rare opportunistic pathogen in humans and animals, especially with biofilm. METHODS: In one case, E. fergusonii with biofilm was detected in the bile, and silver staining was used to prove it had biofilm. The clinical characteristics and drug susceptibility of eight cases of E. fergusonii retrieved from the literature were also summarized. RESULTS: This is a case of E. fergusonii with biofilm, which has not been reported in China. The 8 cases retrieved from the literature did not specify whether they had biofilm, but we analyzed their clinical characteristics and drug susceptibility. All patients were treated with antimicrobial drugs. 8 cases showed sensitivity to piperacillin/tazobactam and imipenem in 6 cases (75%), but poor sensitivity to levofloxacin and ciprofloxacin. CONCLUSION: The silver staining method proved biofilm in this case, which is the first case of E. fergusonii with biofilm in China.


Assuntos
Antibacterianos , Anti-Infecciosos , Animais , Humanos , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Escherichia , Biofilmes , Testes de Sensibilidade Microbiana
5.
Nat Biotechnol ; 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36702897

RESUMO

The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minimal or no modification to the optical system. We show that placing a mirror directly opposite the sample enables four-beam interference with higher spatial frequency content than 3D SIM illumination, offering near-isotropic imaging with ∼120-nm lateral and 160-nm axial resolution. We also developed a deep learning method achieving ∼120-nm isotropic resolution. This method can be combined with denoising to facilitate volumetric imaging spanning dozens of timepoints. We demonstrate the potential of these advances by imaging a variety of cellular samples, delineating the nanoscale distribution of vimentin and microtubule filaments, observing the relative positions of caveolar coat proteins and lysosomal markers and visualizing cytoskeletal dynamics within T cells in the early stages of immune synapse formation.

6.
Food Chem ; 411: 135503, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36682165

RESUMO

The effects of cellulase hydrolysis separately combined with hydroxypropylation, carboxymethylation and phosphate crosslinking on the physicochemical properties and antioxidant activity of millet bran dietary fibre (MBDF) were investigated. Compared to cellulase hydrolysis alone, these dual modifications more effectively improved the soluble fibre content, water-swelling ability, viscosity, emulsifying capacity and cation-exchange capacity of MBDF but reduced the emulsion stability, brightness and polyphenol content of MBDF (P < 0.05). MBDF modified by cellulase hydrolysis combined with hydroxypropylation showed the highest emulsifying capacity (60.03 m2/g) and oil-adsorption capacity (3.32 g/g) but the lowest nitrite ion-adsorbing ability (NIAA). MBDF modified by cellulase hydrolysis with carboxymethylation showed the highest surface hydrophobicity, cation-exchange capacity (0.352 mmol/g) and NIAA (152.89 µg/g). MBDF modified by cellulase hydrolysis combined with phosphate crosslinking exhibited excellent copper ion-adsorbing ability (19.97 mg/g) and viscosity (19.33 cp). Moreover, these dual modifications all enhanced the Fe2+ chelating ability and reducing power of MBDF (P < 0.05).

7.
Ecotoxicol Environ Saf ; 251: 114565, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36682183

RESUMO

Microplastics (MPs) and bisphenol AF (BPAF) are two environmental pollutants that usually coexist in the natural environment. Studies of MPs or BPAF have gradually increased in recent years, but few studies have focused on the combination toxic effects. In this study, the subchronic model of adult zebrafish was exposed to 1 mg/L nanolevel microplastics and 200 µg/L BPAF for 45 days; the parental zebrafish were spawning every 3 days during exposure, and the effects of continuous poisoning were examined on the offspring after 1-9 spawns. The results showed that single BPAF exposure or BPAF and nanoplastic coexposure can both decrease the number of eggs laid and the locomotor behavior of parental zebrafish and impact the hatching rate, mortality, body length and locomotor behavior of offspring zebrafish, especially in 7-9 spawn. BPAF were accumulated in parental zebrafish intestinal in 334.62 ng/g in BPAF group and 594.52 ng/g in nm+BPAF group, and accumulated in whole offspring zebrafish for 281.6 ng/g in BPAF group and 321.46 ng/g in nm+BPAF group. Neurodevelopmental, inflammation, apoptosis and oxidative stress-related genes were also significantly increased after 7-9 spawn. In addition, the exacerbated accumulation in the BPAF+nm group in parental and offspring zebrafish may be the reason for the accelerated toxic effect in the present research. In this study, we investigated the combined effects of nanoplastics and BPAF on parental and offspring zebrafish in the aquatic environment to identify the accumulative toxic effects and provide new experimental support for assessing the effects of coexposure on aquatic organisms.

8.
Photoacoustics ; 29: 100449, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36654963

RESUMO

To realize the real-time highly sensitive detection of SF6 decomposition product H2S, a multi-mechanism collaboration enhancement photoacoustic spectroscopy analyzer (MCEPA) based on acoustic resonance enhancement, cantilever enhancement and excitation light enhancement is proposed. An SF6 background gas-induced photoacoustic cell (PAC) was used for acoustic resonance (AR) enhancement of the photoacoustic signals. A fiber-optic acoustic sensor based on a silicon cantilever is optimized and fabricated. The narrow-band acoustic signal enhancement based on cantilever mechanical resonance (MR) is realized in the optimal working frequency band of the PAC. A fiber-coupled DFB cascaded an Erbium-doped fiber amplifier (EDFA) realized the light power enhancement (LPE) of the photoacoustic signals excitation source. Experimental results show that the MR of the fiber-optic silicon cantilever acoustic sensor (FSCAS) is matched with the AR of the PAC and combined with the LPE, which realizes the multi-mechanism collaboration enhancement of weak photoacoustic signals. The Allan-Werle deviation evaluation showed that the minimum detection limit of H2S in the SF6 background is 10.96 ppb when the average time is 200 s. Benefiting from the all-optimization of photoacoustic excitation and detection, the MCEPA has near-field high-sensitivity gas detection capability immune to electromagnetic interference.

9.
Chin J Nat Med ; 21(1): 36-46, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36641231

RESUMO

Bavachin is a dihydroflavonoid compound isolated from Psoralea corylifolia, and exhibits anti-bacterial, anti-inflammatory, anti-tumor and lipid-lowering activities. Recent attention has gradually drawn on bavachin-induced apoptosis in many human cancer cell lines. However, the anti-cancer effects and related mechanisms in colorectal cancer remain unknown. Here, we investigated the effects of bavachin on colorectal cancer in vivo and in vitro. The results showed that bavachin inhibited the proliferation of human colorectal cancer cells and induce apoptosis. These changes were mediated by activating the MAPK signaling pathway, which significantly up-regulated the expression of Gadd45a. Furthermore, Gadd45a silencing obviously attenuated bavachin-mediated cell apoptosis. Inhibition of the MAPK signaling pathway by JNK/ERK/p38 inhibitors also weakened the up-regulation of Gadd45a by bavachin. The anticancer effect of bavachin was also validated using a mouse xenograft model of human colorectal cancer. In conclusion, these findings suggest that bavachin induces the apoptosis of colorectal cancer cells through activating the MAPK signaling pathway.


Assuntos
Neoplasias Colorretais , Transdução de Sinais , Humanos , Flavonoides/farmacologia , Proteínas/metabolismo , Proteínas/farmacologia , Sistema de Sinalização das MAP Quinases , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Apoptose , Linhagem Celular Tumoral , Proliferação de Células , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Proteínas de Ciclo Celular/farmacologia
10.
PLoS One ; 18(1): e0280378, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36649363

RESUMO

BACKGROUND: Recent studies have reported an association between chronic renal failure and hearing impairment. Yet, the exact mechanism of action is still not fully understood. In this study, we investigated the expression of fibroblast growth factor 23 (FGF23) and D-serine in maintenance hemodialysis (MHD) patients with end-stage renal disease (ESRD) complicated with hearing impairment and further investigated the correlation between FGF23/D-serine and hearing impairment. METHODS: A total of 90 subjects, including 30 MHD patients complicated with hearing impairment, 30 MHD patients with normal hearing, and 30 controls, were included in this case-control study. Relevant data were obtained by questionnaire survey, audiometric test, enzyme-linked immunosorbent assay (ELISA) to determine FGF23 level, and high-performance liquid chromatography to determine D-serine level. RESULTS: MHD patients showed abnormally high expression of FGF23 and D-serine, where FGF23 and D-serine levels were significantly higher in the group with hearing impairment than in the group with normal hearing and normal controls (all P<0.01). Also, elevated FGF23 and D-serine were identified as risk factors for hearing impairment in ESRD, with ORs of 16.54 (95%CI, 2.75-99.55) and 15.22 (95%CI, 2.59-89.51), respectively. Further Person correlation analysis showed a moderate positive correlation between FGF23 and D-serine (r = 0.683, P<0.001). CONCLUSION: This study provides potential biomarkers for the early detection of hearing impairment complicated by chronic renal failure, and the reduction of FGF23/D-serine may provide a potential target for the treatment of hearing impairment complicated by chronic renal failure.


Assuntos
Falência Renal Crônica , Insuficiência Renal Crônica , Humanos , Estudos de Casos e Controles , Correlação de Dados , Fatores de Crescimento de Fibroblastos , Falência Renal Crônica/complicações , Falência Renal Crônica/terapia , Diálise Renal , Insuficiência Renal Crônica/complicações , Serina
11.
J Environ Sci (China) ; 123: 255-269, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36521988

RESUMO

Ambient air quality standards are the core strategic goal of ambient air quality management. Countries worldwide have given importance to research on the development of ambient air quality standards. To understand the history of the development of China's ambient air quality standards, this study analyzed the background associated with all previous formulations and revisions of the standards, classification of functional areas, standard grading, pollutants, and evolution of the standard limits over the past 40 years. The results show that since the initial release of the "Ambient Air Quality Standard" by China in 1982, it has been supplemented once, revised twice, and modified twice. The first ambient air quality standard specified the standard limits of six pollutants commonly found in ambient air. With the development of ambient air quality management, the number of pollutants has increased to ten. Since the release of the "Ambient Air Quality Standards" in 2012, the ambient air quality in China has significantly improved. However, the proportion of cities meeting these standards is still low. At present, China is suitably positioned to make the standards associated with 24 hr SO2 concentrations more stringent such that it meets the values defined in the World Health Organization (WHO) interim target-2 and the WHO air quality guideline (AQG). We further suggested that the SO2 standard should be revised promptly. Simultaneously, regions with a relatively high proportion of cities meeting the standard are encouraged to introduce more stringent interim target limits in due course to manage the local ambient air quality.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Material Particulado/análise , Poluição do Ar/análise , Cidades , China
12.
Zhen Ci Yan Jiu ; 47(11): 941-48, 2022 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-36453669

RESUMO

OBJECTIVE: To observe the effect of acupuncture on microglia polarization and inflammatory reaction in rats with cerebral ischemia-reperfusion injury (CIRI), so as to explore its mechanisms underlying improvement of CIRI. METHODS: Thirty male SD rats were randomly divided into sham operation, model, and acupuncture groups, with 10 rats in each group. The CIRI model was established by occlusion of the middle cerebral artery (MCAO) for 1 h, followed by reperfusion. After modeling, rats in the acupuncture group received manual acupuncture stimulation of "Dazhui" (GV14), "Baihui"(GV20), "Shuigou" (GV26), bilateral "Zusanli" (ST36) and "Fengchi" (GB20) by twirling the needles rapidly for 10 s/acupoint every 10 min, with the needles retained for 20 min. The treatment was conducted once daily for successive 7 days. The neurological function was evaluated according to Longa's method. The state of CIRI was observed after Nissl staining, and the expression levels of Iba-1, iNOS, Arg1, BDNF, GDNF and NeuN in the ischemic cortex tissue were detected by immunofluorescence staining. The contents of TNF-α, IL-6 and IL-10 in the ischemic tissue were assayed by ELISA. The protein expression levels of BDNF, GDNF, TLR4, MyD88 and NF-κB in the ischemic tissues were detected by Western blot. RESULTS: The neurological deficit score on the 24 h and 7th day was considerably higher in the model group than in the sham operation group (P<0.01), and evidently lower on the 7th day in the acupuncture group than in the model group (P<0.01). The number of NeuN positive cells,the area of immunofluorescence dual labelling of Arg1, BDNF and GDNF positive staining, IL-10 content, BDNF and GDNF protein expressions were significantly decreased (P<0.01), and the immunofluorescence dual labelling area of Iba-1 and iNOS, TNF-α and IL-6 contents, the pretein expression levels of TLR4, MyD88 and NF-κB considerably increased (P<0.01) in the model group relevant to the sham operation group. In contrast to the model group, the acupuncture group had a significant increase in the number of NeuN positive cells, the immunofluorescence dual labelling area of Arg1, BDNF and GDNF positive staining, IL-10 content, and BDNF and GDNF protein expressions (P<0.05, P<0.01), and an evident decrease in Iba-1 and iNOS positive staining, contents of TNF-α and IL-6, and the protein expression levels of TLR4, MyD88 and NF-κB (P<0.01, P<0.05). Nissl staining showed a marked reduction in the number of neurons, the nucleus pyknosis and nissl bodies and loose arrangement of the neuronal cells in the model group, which was relatively milder in the acupuncture group. CONCLUSION: Acupuncture intervention can improve neurological function in CIRI rats, which may be related to its effects in regulating the polarization of microglia, reducing inflammatory reaction and increasing the secretion of neurotrophic factors in the brain, inhibiting TLR4/MyD88/NF-κB signaling pathway.


Assuntos
Terapia por Acupuntura , Traumatismo por Reperfusão , Masculino , Animais , Ratos , Ratos Sprague-Dawley , Interleucina-10/genética , Microglia , NF-kappa B/genética , Fator de Necrose Tumoral alfa , Fator Neurotrófico Derivado do Encéfalo , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Interleucina-6 , Fator 88 de Diferenciação Mieloide/genética , Receptor 4 Toll-Like/genética , Infarto Cerebral , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/terapia
13.
Cereb Cortex ; 2022 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-36482789

RESUMO

It has been shown that the functional dependency of the brain exists in both direct and indirect regional relationships. Therefore, it is necessary to map higher-order coupling in brain structure and function to understand brain dynamic. However, how to quantify connections between not directly regions remains unknown to schizophrenia. The word2vec is a common algorithm through create embeddings of words to solve these problems. We apply the node2vec embedding representation to characterize features on each node, their pairwise relationship can give rise to correspondence relationships between brain regions. Then we adopt pearson correlation to quantify the higher-order coupling between structure and function in normal controls and schizophrenia. In addition, we construct direct and indirect connections to quantify the coupling between their respective functional connections. The results showed that higher-order coupling is significantly higher in schizophrenia. Importantly, the anomalous cause of coupling mainly focus on indirect structural connections. The indirect structural connections play an essential role in functional connectivity-structural connectivity (SC-FC) coupling. The similarity between embedded representations capture more subtle network underlying information, our research provides new perspectives for understanding SC-FC coupling. A strong indication that the structural backbone of the brain has an intimate influence on the resting-state functional.

14.
Front Nutr ; 9: 1005308, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36505260

RESUMO

Introduction: Autism spectrum disorder (ASD) is a serious neurodevelopmental disorder with a rising incidence. More and more studies have shown that abnormal microbiota composition may aggravate the behavioral symptoms and biological signs of ASD, and interventions of probiotics and diet have emerged as a potential improvement measure. Methods: Lactiplantibacillus plantarum ST-III-fermented milk was applied as an oral intervention in a valproic acid (VPA)-induced ASD mice model, and the effect of probiotic intake on autistic-related behaviors and gut microbiota composition was evaluated by behavioral tests and 16S rRNA gene sequencing. Results: Gender specificity was shown in VPA-induced behavioral abnormalities in a mouse model, and L. plantarum ST-III-fermented milk was effective in ameliorating the impaired social interaction in male ASD mouse models, but not for the anxiety behavior exhibited by female ASD mouse models. Meanwhile, dietary changes were found to be the main cause of the altered gut microbiota in mice, and additional intake of L. plantarum ST-III-fermented milk seemed to improve autistic-like behaviors in male ASD mouse models by modulating specific gut microbes. Discussion: These findings suggest that L. plantarum ST-III-fermented milk may play a beneficial role in improving the behavioral symptoms of ASD and is expected to be one of the candidate functional foods for ASD.

15.
RSC Adv ; 12(52): 33584-33588, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36505720

RESUMO

A metal-free catalytic oxidation of α-diazoesters via a green environmental-friendly route was developed. The α-diazoesters were converted to α-ketoesters using DMF and molecular oxygen as oxygen sources and B(C6F5)3 as the catalyst, without any additives. This protocol has a broad adaptability of substrates and good compatibility with a range of functional groups, and it offers new insight into reactions catalyzed by B(C6F5)3.

16.
Discov Oncol ; 13(1): 138, 2022 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-36512117

RESUMO

Lung adenocarcinoma is the major subtype of lung cancer, accounting for approximately 40% of lung cancers. During clinical treatment, the emergence of chemotherapy resistance seriously affects the effectiveness of treatment. Thus, finding new chemotherapeutic sensitizers is considered to be one of the effective solutions. Biochanin A, as a naturally occurring isoflavone, has been demonstrated to exhibit anticancer effects in various tumors. However, the potential mechanisms of Biochanin A to inhibit tumor development have not been clarified. In the present study, we found that the combinational treatment of cisplatin and Biochanin A exhibited strong synergistic repression on lung adenocarcinoma growth and progression in vitro and in vivo. Considering that epithelial-mesenchymal transition (EMT) is recognized to be associated with both chemoresistance and metastasis, we examined the EMT-related markers and found that Biochanin A could specifically inhibit the expression of ZEB1. Importantly, Biochanin A chemosensitizes lung adenocarcinoma and inhibits cancer cell metastasis by suppressing ZEB1. At the molecular level, Biochanin A affects the stability of ZEB1 protein through the deubiquitination pathway and thereby influences the progression of lung adenocarcinoma. In conclusion, our finding elucidates the potential efficacy of Bichanin A as a chemosensitizer and provides new strategy for the chemotherapy of advanced lung adenocarcinoma.

17.
Ann Noninvasive Electrocardiol ; : e13024, 2022 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-36573893

RESUMO

OBJECTIVE: This study aimed to analyze the conventional surface electrocardiogram (ECG) characteristics of premature ventricular contractions (PVCs) originating from the tricuspid annulus and to investigate the efficacy of locating their origins according to ECG results. METHODS: Eight patients who underwent radiofrequency ablation in the First Hospital of Shanxi Medical University (China) were included in the study. Pace mapping (PM) was used to analyze the characteristics of the PVCs originating from the tricuspid annulus recorded via 12-lead body surface ECGs. RESULTS: An R-wave was found in leads I, V5 , and V6 . The QRS wave was narrower when the PVCs originated from the septum and shifted in lead V3 (R-wave amplitude/S-wave amplitude in the precordial lead-1). The QRS wave was broadest when the PVCs originated from the 7 to 9 o'clock position. The augmented vector left lead showed RS, QS, or RSR-type waves with a low amplitude when the PVCs originated from the upper part of the annulus. When the PVCs originated from the lower part of the annulus, the augmented vector right lead reflected multidirectional and QS-type waves. CONCLUSION: The ECG-lead characteristics related to the origin of PVCs in the tricuspid annulus indicate some level of significance and can be used to formulate a specific diagnosis.

18.
J Int Med Res ; 50(12): 3000605221142403, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36482662

RESUMO

In this article, an unusual case of a 27-year-old woman, who presented with abdominal pain for the previous 2 days, is presented. Ultrasonography revealed a perforated uterus and a straw in the abdominal cavity. A foreign body in the abdominal cavity was diagnosed and removed by laparoscopic surgery, and antibiotics were administered. The patient reported no discomfort during follow-up for 1 month. This report highlights the rare case of a foreign body crossing the uterus into the abdominal cavity. The only possible action was immediate surgery to remove the foreign body from the abdominal cavity.


Assuntos
Cavidade Abdominal , Corpos Estranhos , Feminino , Humanos , Adulto , Cavidade Abdominal/diagnóstico por imagem , Cavidade Abdominal/cirurgia , Corpos Estranhos/diagnóstico por imagem , Corpos Estranhos/cirurgia
19.
Sci Rep ; 12(1): 19124, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36352047

RESUMO

The sealing ring of the external floating roof tank is prone to petroleum gas leakage due to material aging and oil corrosion. Petroleum gas leakage and diffusion easily accumulate above the floating deck. When it is within the explosion limit range, there will be the risk of explosion and fire. To deal with the explosion accident of storage tank caused by the concentration distribution of petroleum gas leakage for the sealing ring, and to study the influence of petroleum gas diffusion and concentration distribution after sealing ring leakage on the control area above the floating deck in the tank farm environment, this paper established numerical models of sealing ring leakage under different liquid level heights for 10 × 104 m3 external floating roof tank. Through numerical calculation, it is found that the diffusion concentration of petroleum gas is related to the wind speed, the range of the control area above the floating deck, and leakage when sealing rings leak at different liquid levels. Through dimensionless analysis, the functional relationship of gas leakage diffusion concentration distribution under different liquid level heights of external floating roof tank sealing rings is verified by numerical calculation results. The results show that the numerical results are consistent with those predicted by the formula.

20.
J Cell Physiol ; 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36409765

RESUMO

Colorectal cancer (CRC) is the malignant tumor with the highest incidence in the digestive system, and the gut microbiome plays a crucial role in CRC tumorigenesis and therapy. The gastrointestinal tract is the organ harboring most of the microbiota in humans. Changes in the gut microbiome in CRC patients suggest possible host-microbe interactions, thereby hinting the potential tumorigenesis, which provides new perspective for preventing, diagnosing, or treating CRC. In this review, we discuss the effects of gut microbiome dysbiosis on CRC, and reveal the mechanisms by which gut microbiome dysbiosis leads to CRC. Gut microbiome modulation with the aim to reverse the established gut microbial dysbiosis is a novel strategy for the prevention and treatment of CRC. In addition, this review summarizes that probiotic antagonize CRC tumorigenesis by protecting intestinal barrier function, inhibiting cancer cell proliferation, resisting oxidative stress, and enhancing host immunity. Finally, we highlight clinical applications of the gut microbiome, such as gut microbiome analysis-based biomarker screening and prediction, and microbe modulation-based CRC prevention, treatment enhancement, and treatment side effect reduction. This review provides the reference for the clinical application of gut microbiome in the prevention and treatment of CRC.

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