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1.
Hum Mol Genet ; 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36708031

RESUMO

Oligoasthenoteratozoospermia (OAT) can result in male infertility due to reduced sperm motility and abnormal spermatozoan morphology. The Tektins are a family of highly conserved filamentous proteins expressed in the axoneme and associated structures in many different metazoan species. Earlier studies on mice identified Tektin3 (Tekt3) as a testis-enriched gene, and knockout of Tekt3 resulted in asthenozoospermia in the mice. Here, whole exome sequencing of 100 males with asthenozoospermia from unrelated families was performed, followed by Sanger sequencing, leading to the identification of TEKT3 as a candidate gene in two of these patients and their associated family members. In total, three mutations in the TEKT3 gene were identified in both these patients, including one homozygous deletion-insertion mutation (c.543_547delinsTTGAT: p.Glu182*) and one compound heterozygous mutation (c.[548G > A]; [752A > C], p.[(Arg183Gln)]; [(Gln251Pro)]). Both of these mutations resulted in the complete loss of TEKT3 expression. The patients were both found to produce sperm that, although they showed no apparent defects in the flagellar structure, had reduced progressive motility. In contrast to mice, most sperm from these two patients exhibited acrosomal hypoplasia, although this did not prevent the use of the sperm for in vitro fertilization through an ICSI approach. TEKT3 was found to bind to other TEKT proteins, suggesting that these proteins form a complex within human spermatozoa. Overall, these results suggest that a loss of TEKT3 function can contribute to OAT incidence in humans. TEKT3 deficiencies can reduce sperm motility and contribute to severe acrosomal hypoplasia in spermatozoa, compromising their normal function.

2.
Int J Mol Sci ; 24(2)2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36674732

RESUMO

MiRNAs are critical regulators of numerous physiological and pathological processes. Ascosphaera apis exclusively infects bee larvae and causes chalkbrood disease. However, the function and mechanism of miRNAs in the bee larval response to A. apis infection is poorly understood. Here, ame-miR-34, a previously predicted miRNA involved in the response of Apis mellifera larvae to A. apis invasion, was subjected to molecular validation, and overexpression and knockdown were then conducted to explore the regulatory functions of ame-miR-34 in larval body weight and immune response. Stem-loop RT-PCR and Sanger sequencing confirmed the authenticity of ame-miR-34 in the larval gut of A. mellifera. RT-qPCR results demonstrated that compared with that in the uninfected larval guts, the expression level of ame-miR-34 was significantly downregulated (p < 0.001) in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae, indicative of the remarkable suppression of host ame-miR-34 due to A. apis infection. In comparison with the corresponding negative control (NC) groups, the expression level of ame-miR-34 in the larval guts in the mimic-miR-34 group was significantly upregulated (p < 0.001), while that in the inhibitor-miR-34 group was significantly downregulated (p < 0.01). Similarly, effective overexpression and knockdown of ame-miR-34 were achieved. In addition, the body weights of 5- and 6-day-old larvae were significantly increased compared with those in the mimic-NC group; the weights of 5-day-old larvae in the inhibitor-miR-34 group were significantly decreased in comparison with those in the inhibitor-NC group, while the weights of 4- and 6-day-old larvae in the inhibitor-miR-34 group were significantly increased, indicating the involvement of ame-miR-34 in modulating larval body weight. Furthermore, the expression levels of both hsp and abct in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae were significantly upregulated after ame-miR-34 overexpression. In contrast, after ame-miR-34 knockdown, the expression levels of the aforementioned two key genes in the A. apis-infected 4-, 5-, and 6-day-old larval guts were significantly downregulated. Together, the results demonstrated that effective overexpression and knockdown of ame-miR-34 in both noninfected and A. apis-infected A. mellifera larval guts could be achieved by the feeding method, and ame-miR-34 exerted a regulatory function in the host immune response to A. apis invasion through positive regulation of the expression of hsp and abct. Our findings not only provide a valuable reference for the functional investigation of bee larval miRNAs but also reveal the regulatory role of ame-miR-34 in A. mellifera larval weight and immune response. Additionally, the results of this study may provide a promising molecular target for the treatment of chalkbrood disease.


Assuntos
Arthrodermataceae , Abelhas , MicroRNAs , Animais , Abelhas/genética , Abelhas/imunologia , Abelhas/microbiologia , Peso Corporal , Imunidade , Larva/imunologia , MicroRNAs/genética , MicroRNAs/metabolismo , Arthrodermataceae/fisiologia
3.
Science ; 379(6629): 288-294, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36656941

RESUMO

High-quality perovskite light harvesters and robust organic hole extraction layers are essential for achieving high-performing perovskite solar cells (PSCs). We introduce a phosphonic acid-functionalized fullerene derivative in mixed-cation perovskites as a grain boundary modulator to consolidate the crystal structure, which enhances the tolerance of the film against illumination, heat, and moisture. We also developed a redox-active radical polymer, poly(oxoammonium salt), that can effectively p-dope the hole-transporting material by hole injection and that also mitigates lithium ion diffusion. Power conversion efficiencies of 23.5% for 1-square-centimeter mixed-cation-anion PSCs and 21.4% for 17.1-square-centimeter minimodules were achieved. The PSCs retained 95.5% of their initial efficiencies after 3265 hours at maximum power point tracking under continuous 1-sun illumination at 70° ± 5°C.

4.
Antioxidants (Basel) ; 12(1)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36671067

RESUMO

Ascosphaera apis infects exclusively bee larvae and causes chalkbrood, a lethal fungal disease that results in a sharp reduction in adult bees and colony productivity. However, little is known about the effect of A. apis infestation on the activities of antioxidant enzymes in bee larvae. Here, A. apis spores were purified and used to inoculate Asian honey bee (Apis cerana) larvae, followed by the detection of the host survival rate and an evaluation of the activities of four major antioxidant enzymes. At 6 days after inoculation (dpi) with A. apis spores, obvious symptoms of chalkbrood disease similar to what occurs in Apis mellifera larvae were observed. PCR identification verified the A. apis infection of A. cerana larvae. Additionally, the survival rate of larvae inoculated with A. apis was high at 1-2 dpi, which sharply decreased to 4.16% at 4 dpi and which reached 0% at 5 dpi, whereas that of uninoculated larvae was always high at 1~8 dpi, with an average survival rate of 95.37%, indicating the negative impact of A. apis infection on larval survival. As compared with those in the corresponding uninoculated groups, the superoxide dismutase (SOD) and catalase (CAT) activities in the 5- and 6-day-old larval guts in the A. apis-inoculated groups were significantly decreased (p < 0.05) and the glutathione S-transferase (GST) activity in the 4- and 5-day-old larval guts was significantly increased (p < 0.05), which suggests that the inhibition of SOD and CAT activities and the activation of GST activity in the larval guts was caused by A. apis infestation. In comparison with that in the corresponding uninoculated groups, the polyphenol oxidase (PPO) activity was significantly increased (p < 0.05) in the 5-day-old larval gut but significantly reduced (p < 0.01) in the 6-day-old larval gut, indicating that the PPO activity in the larval guts was first enhanced and then suppressed. Our findings not only unravel the response of A. cerana larvae to A. apis infestation from a biochemical perspective but also offer a valuable insight into the interaction between Asian honey bee larvae and A. apis.

5.
Bioeng Transl Med ; 8(1): e10364, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36684070

RESUMO

Cartilage regeneration after injury is still a great challenge in clinics, which suffers from its avascularity and poor proliferative ability. Herein we designed a novel biocompatible cellulose nanocrystal/GelMA (gelatin-methacrylate anhydride)/HAMA (hyaluronic acid-methacrylate anhydride)-blended hydrogel scaffold system, loaded with synthetic melanin nanoparticles (SMNP) and a bioactive drug kartogenin (KGN) for theranostic purpose. We found that the SMNP-KGN/Gel showed favorable mechanical property, thermal stability, and distinct magnetic resonance imaging (MRI) contrast enhancement. Meanwhile, the sustained release of KGN could recruit bone-derived mesenchymal stem cells to proliferate and differentiate into chondrocytes, which promoted cartilage regeneration in vitro and in vivo. The hydrogel degradation and cartilage restoration were simultaneously monitored by multiparametric MRI for 12 weeks, and further confirmed by histological analysis. Together, these results validated the multifunctional hydrogel as a promising tissue engineering platform for noninvasive imaging-guided precision therapy in cartilage regenerative medicine.

6.
Heliyon ; 9(1): e12666, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36685422

RESUMO

Background: The effects of realgar against non-small cell lung cancer (NSCLC) have been massively studied, but the direct therapeutic targets of realgar remain unclear. This study aimed to identify the molecular targets of realgar against NSCLC and explore their therapeutic mechanisms based on a network pharmacology approach and experimental validations. Methods: The BATMAN-TCM and Digsee databases were used to predict realgar targets and NSCLC-related genes, respectively. A protein-protein interaction network was constructed for each gene set, and the overlapping genes were identified as potential targets of realgar against NSCLC. The correlation between potential targets and NSCLC was analyzed using The Cancer Genome Atlas and International Cancer Genome Consortium databases, and the key target was validated by in-silico and in-vitro experiments. Results: Twenty-three overlapping genes, including xanthine oxidase (XO), were identified as potential targets of realgar against NSCLC. XO was selected as the key target for validation, as it was found to be upregulated in NSCLC tumor tissue, which correlated with poor overall survival. A possible interaction between realgar and XO was revealed by molecular docking which was further validated experimentally. Realgar treatment suppressed the activity of XO in NSCLC cells, as demonstrated by the unchanged XO protein levels. Finally, the mechanism of action of XO as a target against NSCLC through the cell-cell junction organization pathway was investigated. Conclusions: Overall, this study proposes a potential molecular mechanism illustrating that XO is a target of realgar against NSCLC and highlights the usefulness of XO as a therapeutic target for NSCLC.

7.
Small ; : e2207767, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624608

RESUMO

In recent years, three-dimensionally ordered macroporous (3DOM) materials have attracted tremendous interest in the field of photocatalysis due to the periodic spatial structure and unique physicochemical properties of 3DOM catalysts. In this review, the fundamentals and principles of 3DOM photocatalysts are briefly introduced, including the overview of 3DOM materials, the photocatalytic principles based on 3DOM materials, and the advantages of 3DOM materials in photocatalysis. The preparation methods of 3DOM materials are also presented. The structure and properties of 3DOM materials and their effects on photocatalytic performance are briefly summarized. More importantly, 3DOM materials, as a supported catalyst, are extensively employed to combine with various common materials, including metal nanoparticles, metal oxides, metal sulfides, and carbon materials, to enhance photocatalytic performance. Finally, the prospects and challenges for the development of 3DOM materials in the field of photocatalysis are presented.

8.
Membranes (Basel) ; 13(1)2023 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-36676885

RESUMO

Reverse osmosis (RO) membranes are susceptible to silica scaling, resulting in irreversible degradation of membrane performance. This work covered the fabrication of MIL-101(Fe) for silicic acid adsorption to alleviate the silica scaling of RO membranes. The effect of pH, mixing time and initial concentration on silicic acid adsorption of MIL-101(Fe) was appraised in detail. The adsorption experiments demonstrated that MIL-101(Fe) possessed an excellent adsorption ability for silicic acid with the maximum adsorption capacity reaching 220.1 mgSiO2·g-1. Data fitting confirmed the pseudo-second-order equation and Freundlich equation were consistent with silicic acid adsorption on MIL-101(Fe). Finally, a simulated anti-scaling experiment was carried out using a feed solution pretreated by MIL-101(Fe) adsorption, and the permeance exhibited a much lower decline after 24 h filtration, confirming that MIL-101(Fe) exhibits an excellent application potential for silica-scale mitigation in RO systems.

9.
Food Funct ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36655918

RESUMO

Dietary oil composition determines the pathological processes of alcoholic fatty liver disease (AFLD). Oil rich in saturated fatty acids protects, whereas oil rich in polyunsaturated fatty acids aggravates the alcohol-induced liver injury. However, limited studies have been conducted to address how monounsaturated fatty acids (MUFAs) enriched oil controls the pathological development of AFLD. Therefore, this study was designed to evaluate the effect of MUFA-enriched extra virgin olive oil (OO) on AFLD. Twenty C57BL/6J mice were randomly allocated into four groups and fed modified Lieber-DeCarli liquid diets containing isocaloric maltose dextrin a non-alcohol or alcohol with corn oil and OO for four weeks. Dietary OO significantly exacerbated alcohol-induced liver dysfunction, evidenced by histological examinations and disturbed biochemical parameters. Dietary OO with alcohol decreased hormone-sensitive lipase (HSL), phosphorylated 5'-AMP-activated protein kinase (p-AMPK), and carnitine palmitoyltransferase-Iα (CPT1α) expression, and increased sterol regulatory element-binding protein-1c (SREBP-1c), diacylglycerol acyltransferase-2 (DGAT2), and very low-density lipoprotein receptor (VLDLR) expression in the liver. It also promoted the expression of hepatic interleukin-6 (IL-6) and hepatic tumour necrosis factor-alpha (TNF-α) at the transcriptional level. Additionally, adipose tissue lipolysis partially had an etiologic effect on alcohol-induced hepatic steatosis under OO pretreatment. In conclusion, MUFA-enriched OO exacerbated liver dysfunction in vivo. OO should be cautiously considered as a unique dietary oil source for individuals with AFLD.

10.
Adv Mater ; : e2211032, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36642975

RESUMO

Li metal, a promising anode for high-energy-density rechargeable batteries, typically grows along the low-surface energy (110) plane in the plating process, resulting in uncontrollable dendrite growth and unstable interface. Herein, we report an unexpected Li growth behavior by lanthanum (La) doping: the preferred orientation turns to (200) from (110) plane, enabling a two-dimensional nuclei rather than usual one-dimensional nuclei upon Li deposition and thus forming a dense and dendrite-free morphology even at an ultra-high-areal-capacity of 10 mAh cm-2 . Noticeably, the La doping further decreases the reactivity of Li metal towards electrolytes, thereby establishing a stable interface. The dendrite-free, stable Li anode enables a high average Coulombic efficiency of 99.30% at 8 mAh cm-2 for asymmetric Li||LaF3 -Cu cells. We further demonstrate a 3.1 Ah LaF3 -Li||LiNi0.8 Co0.1 Mn0.1 O2 pouch cell at a high energy density (425.73 Wh kg-1 ) with impressive cycling stability (0.0989% decay/cycle) under lean electrolyte (1.76 g Ah-1 ) and high cathode loading (5.77 mAh cm-2 ) using this doped Li anode. This article is protected by copyright. All rights reserved.

11.
Sci Rep ; 13(1): 511, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36627326

RESUMO

Tongue cracks refer to fissures with different depth and shapes on the tongue's surface, which can characterize the pathological characteristics of spleen and stomach. Tongue cracks are of great significance to the objective study of tongue diagnosis. However, tongue cracks are small and complex, existing methods are difficult to extract them effectively. In order to achieve more accurate extraction and identification of tongue crack, this paper proposes to apply a deep learning network based on image segmentation (Segmentation-Based Deep-Learning, SBDL) to extract and identify tongue crack. In addition, we have studied the quantitative description of tongue crack features. Firstly, the pre-processed tongue crack samples were amplified by using adding salt and pepper noise, changing the contrast and horizontal mirroring; secondly, the annotation tool Crack-Tongue was used to label tongue crack; thirdly, the tongue crack extraction model was trained by using SBDL; fourthly, the cracks on the tongue surface were detected and located by the segmentation network, and then the output and features of the segmentation network were put into the decision network for the classification of crack tongue images; finally, the tongue crack segmentation and identification results were quantitatively evaluated. The experimental results showed that the tongue crack extraction and recognition results based on SBDL were better than Mask Region-based Convolutional Neural Network (Mask R-CNN), DeeplabV3+, U-Net, UNet++ and Semantic Segmentation with Adversarial Learning (SegAN). This method effectively solved the inaccurate tongue crack extraction caused by the tongue crack's color being close to the surrounding tongue coating's color. This method can achieve better tongue crack extraction and recognition results on a small tongue crack data set and provides a new idea for tongue crack recognition, which is of practical value for tongue diagnosis objectification.


Assuntos
Aprendizado Profundo , Língua/diagnóstico por imagem , Excipientes , Alimentos , Redes Neurais de Computação , Processamento de Imagem Assistida por Computador
12.
Int J Biol Sci ; 19(2): 393-411, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36632462

RESUMO

Asthenoteratozoospermia is one of the major factors for male infertility, whereas the causes of large numbers of cases are still unknown. We identified compound heterozygous variants of FSIP2 in three unrelated individuals from a cohort of 105 patients with asthenoteratozoospermia by exome sequencing. Deleterious FSIP2 variations caused severe disassembly of the fibrous sheath and axonemal defects. Intriguingly, spermatozoa in our study manifested "super-length" mitochondrial sheaths, increased levels of the mitochondrial sheath outer membrane protein TOMM20 and decreased mitochondrial ATP consumption. Dislocation or deletion of the annulus and reduction or dislocation of the annulus protein SEPT4 were also observed. While the lengthened mitochondrial sheaths were not presented in men harboring SEPT4 variants. Furthermore, female partners of two of three men achieved successful pregnancies following intracytoplasmic sperm injection (ICSI). Overall, we presume that FSIP2 may not only serve as a structural protein of the fibrous sheath but also as an intra-flagellar transporter involving in the axonemal assembly, mitochondrial selection and the termination of mitochondrial sheath extension during spermatogenesis, and ICSI is an effective treatment for individuals with FSIP2-associated asthenoteratozoospermia.


Assuntos
Astenozoospermia , Infertilidade Masculina , Gravidez , Humanos , Masculino , Feminino , Astenozoospermia/genética , Sêmen , Espermatozoides , Espermatogênese , Proteínas
13.
Artigo em Inglês | MEDLINE | ID: mdl-36634370

RESUMO

Recently, two-dimensional (2D) Indium Selenide (InSe) has been receiving much attention in the scientific community due to its reduced size, extraordinary physical properties, and potential applications in various fields. In this review, we discussed the recent research advancement in the carrier and phonon transport properties of 2D InSe and its related Janus structures. We first introduced the progress in the synthesis of 2D InSe. We summarized the recent experimental and theoretical works on the carrier mobility, thermal conductivity, and thermoelectric characteristics of 2D InSe. Based on the Boltzmann transport equation, the mechanisms underlying carrier or phonon scattering of 2D InSe were discussed in detail. Moreover, the structural and transport properties of Janus structures based on InSe were also presented, with emphasis on the theoretical simulations. At last, we discussed the prospects for continued research of 2D InSe.

15.
World J Clin Cases ; 11(1): 172-176, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36687185

RESUMO

BACKGROUND: The levonorgestrel-releasing intrauterine system (LNG-IUS) is widely used in contraception, menorrhagia, dysmenorrhea and to prevent endometrial hyperplasia during estrogen supplementation. Perforation is more often seen after early postpartum placement. Perforation of the LNG-IUS occurring one month after placement is rare. CASE SUMMARY: A 42-year-old female complained of progressive dysmenorrhea and increased menstrual volume. She was diagnosed with adenomyosis and the LNG-IUS was inserted in her uterine cavity. Routine ultrasound examination one month later revealed that the intra-uterine device (IUD) was not found in the uterine cavity, and further X-ray and pelvic magnetic resonance imaging showed an abnormal signal area in the left posterior region of the uterus. Laparoscopic exploratory surgery was performed and the LNG-IUS was found in the left uterosacral ligament. CONCLUSION: Perforation of a LNG-IUS occurring one month after placement is rare, and is more common in inexperienced operators and after early postpartum placement. When the operation is difficult, ultrasound monitoring is recommended to reduce the risk of IUD perforation. For patients with inadequate surgery, postoperative imaging is recommended to detect potential risks as soon as possible.

16.
Plant Physiol Biochem ; 194: 406-417, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36493589

RESUMO

Plant growth, development, yield and quality are limited by barren soil. Soil phosphorus deficiency is one of the common factors causing soil barrenness. Plants have evolved morphological, physiological and molecular adaptations to resist to phosphorus deficiency. Wild soybean, a wild relative of cultivated soybean, has an obvious genetic relationship with cultivated soybean and has many beneficial characteristics such as strong low phosphorus resistance. Therefore, in this study, the integration analysis of transcriptome and metabolome of wild and cultivated soybean seedlings leaves were applied under phosphorus deficiency to reveal the mechanism of resistance to low phosphorus stress in wild soybean leaves, especially the key role of membrane phospholipid reuse and protection. Under phosphorus deficiency, wild soybean resisted low phosphorus stress by enhancing phosphorus reuse and strengthening membrane protection mechanisms, that is, by enhancing phospholipid metabolism, degrading membrane phospholipids, releasing phosphorus, increasing phosphorus reuse, and enhancing galactolipid biosynthesis. This, in turn, produced digalactosyl diacylglycerol to replace missing phospholipids for membrane maintenance and enhanced glutathione metabolism to protect the membrane system from damage. At the same time, phosphorus deficiency increased the levels of the intermediate metabolites glycine and ornithine, while significantly regulating the expression of transcription factors WRKY75 and MYB86. The enhancement of these metabolic pathways and the significant regulation of gene expression play an important role in improving the low phosphorus tolerance of wild soybean. This study will provide a useful theoretical basis for breeding soybean with low phosphorus tolerance.


Assuntos
Fabaceae , Soja , Soja/metabolismo , Transcriptoma , Plântula/metabolismo , Metabolômica , Melhoramento Vegetal , Metaboloma , Fabaceae/metabolismo , Folhas de Planta/metabolismo , Fósforo/metabolismo , Solo , Regulação da Expressão Gênica de Plantas
17.
Bioact Mater ; 24: 69-80, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36582352

RESUMO

Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds or even severe tissue damage. In this study, we developed a controlled-release antibacterial composite hydrogel system that can promote wound angiogenesis and inhibit inflammation by sustained releasing Cu-Epigallocatechin-3-gallate (Cu-EGCG) nano-capsules. The prepared SilMA/HAMA/Cu-EGCG hydrogel showed an obvious inhibitory effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). It could also promote the proliferation and migration of L929 fibroblasts. In vivo full-thickness infected wound healing experiments confirmed the angiogenesis and inflammation regulating effect. Accelerate collagen deposition and wound healing speed were also observed in the SilMA/HAMA/Cu-EGCG hydrogel treated group. The findings of this study show the great potential of this controlled-release antibacterial composite hydrogel in the application of chronic wound healing.

19.
Front Mol Biosci ; 9: 1019899, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36458094

RESUMO

Background and Objective: Although growing evidence indicates that non-alcoholic fatty liver disease is related to diabetic retinopathy (DR), research results significantly vary. Therefore, we conducted a meta-analysis to assess the association between the progression of non-alcoholic fatty liver disease and the onset of DR. Methods: PubMed, Embase, and Cochrane databases were searched until 7 November 2021. Combined odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the association. Results: We identified 18 studies involving 12,757 patients. The pooled effect assessment showed that liver fibrosis was positively correlated with DR (OR = 1.69, 95%CI 1.30-2.20; p < 0.0001); non-alcoholic fatty liver disease was not associated with the risk of DR (OR = 1.15, 95%CI 0.75-1.76; p = 0.51); non-alcoholic fatty liver disease was positively correlated with DR in patients with type 1 diabetes (OR = 2.96, 95%CI 1.48-5.94; p = 0.002). In patients with type 2 diabetes, there was no association between non-alcoholic fatty liver disease and DR (OR = 0.92, 95%CI 0.59-1.43; p = 0.70). Subgroup analysis showed no correlation in both Asian and Caucasian races. Conclusion: There is a significant correlation between liver fibrosis and DR. This suggests that the ocular examination of DR could be helpful in predicting whether patients with non-alcoholic fatty liver disease would progress to liver fibrosis.

20.
Pestic Biochem Physiol ; 188: 105285, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36464328

RESUMO

The oriental fruit fly, Bactrocera dorsalis (Hendel), is a notoriously agricultural pest that causes serious economic losses to fruits and vegetables. Widespread insecticide resistance in B. dorsalis is a major obstacle in successful control. Therefore, new pest control strategies, such as those targeting specific genes that can block pest development, are urgently needed. In the current study, the function of JHAMT in B. dorsalis was systematically investigated. A methyltransferase gene in B. dorsalis (BdJHAMT) that is homologous to JHAMT of Drosophila melanogaster was cloned firstly. The subsequently spatiotemporal expression analysis indicated that BdJHAMT mRNA was continuously present in the larval stage, declined sharply immediately before pupation, and then increased in the adult. Subcellular localization showed that BdJHAMT was localized in the adult corpora allata and larval intestinal wall cells. The JH III titer in B. dorsalis was closely related to the transcription level of BdJHAMT in different developmental stages. The dsBdJHAMT feeding-based RNAi resulted in a greatly decreased JH III titer that disrupted fly development. The slow growth caused by BdJHAMT silencing was partially rescued by application of the JH mimic, methoprene. These results demonstrated that BdJHAMT was crucial for JH biosynthesis and thus regulated larval development in B. dorsalis, indicating it may serve as a prospective target for the development of novel control strategies against this pest.


Assuntos
Hormônios Juvenis , Tephritidae , Animais , Hormônios Juvenis/farmacologia , Interferência de RNA , Metiltransferases/genética , Drosophila melanogaster , Tephritidae/genética , Drosophila , Larva/genética
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