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1.
Artigo em Inglês | MEDLINE | ID: mdl-31895669

RESUMO

Background: Infection, including mixed infection, is not uncommon in orthopedic surgical incision. This study aimed to investigate the epidemiology and drug resistance of mixed infections after orthopedic surgical procedures. Methods: We retrospectively analyzed 533 orthopedic surgical site infections (SSIs) in a university hospital from 2012 to 2017. Eighty-six patients (218 strains) with bacterial culture results showing more than one strain were screened to explore their epidemiology and drug resistance. Results: Of 218 bacterial strains, 2-7 bacterial infections were noted in each wound. Most infections were caused by two kinds of bacteria (65.1%). The number of infections decreased with increased number of strains. The combinations of pathogenic micro-organisms were all gram-negative, 55.81%; gram-positive and gram-negative, 30.23%; all gram-positive, 12.79%; and gram-positive and fungi, 1.16%. Their resistance is consistent with the bacterial resistance of 447 cases of single bacterial SSI during the same period. Hospitalization duration was longer (9.8-20.6 d). Conclusion: Our study shows no significant changes in epidemiology and drug resistance caused by mixed infections in the orthopedic surgical site because of coordination and competition among micro-organisms. These bacteria are difficult to control, leading to extended hospitalization. Antibiotic agents should be chosen strictly according to drug sensitivity, and ineffective antibiotic agents must be avoided.

2.
Medicine (Baltimore) ; 98(44): e17740, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689822

RESUMO

To identify independent factors associated with prolonged hospital length of stay (LOS) in elderly patients undergoing first-time elective open posterior lumbar fusion surgery.We retrospectively analyzed the data of 303 elderly patients (age range: 60-86 years) who underwent first-time elective open lumbar posterior fusion surgery at our center from December 2012 to December 2017. Preoperative and perioperative variables were extracted and analyzed for all patients, and multivariate stepwise regression analysis was used to determine the variables affecting the LOS and important predictors of LOS prolongation (P < .001).The mean age of the patients was 67.0 ±â€Š5.5 years, and the mean LOS was 18.5 ±â€Š11.8 days, ranging from 7 to 103 days. Of the total, 166 patients (54.8%) were men and 83 patients (27.4%) had extended LOS. Multiple linear regression analysis determined that age (P < .001), preoperative waiting time ≥7 days (P < .001), pulmonary comorbidities (P = .010), and diabetes (P = .010) were preoperative factors associated with LOS prolongation. Major complications (P = .002), infectious complications (P = .001), multiple surgeries (P < .001), and surgical bleeding (P = .018) were perioperative factors associated with LOS prolongation. Age (P < .001), preoperative waiting time ≥7 days (P < .001), infectious complications (P < .001), and multiple surgeries (P < .001) were important predictors of LOS prolongation.Extended LOS after first-time elective open posterior lumbar fusion surgery in elderly patients is associated with factors including age, preoperative waiting time, infectious complications, and multiple surgeries. Surgeons should recognize and note these relevant factors while taking appropriate precautions to optimize the modifiable factors, thereby reducing the LOS as well as hospitalization costs.


Assuntos
Procedimentos Cirúrgicos Eletivos/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Fusão Vertebral/estatística & dados numéricos , Fatores de Tempo , Tempo para o Tratamento/estatística & dados numéricos , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Procedimentos Cirúrgicos Eletivos/efeitos adversos , Procedimentos Cirúrgicos Eletivos/métodos , Feminino , Humanos , Vértebras Lombares/cirurgia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Complicações Pós-Operatórias/etiologia , Período Pré-Operatório , Análise de Regressão , Estudos Retrospectivos , Fatores de Risco , Fusão Vertebral/efeitos adversos , Fusão Vertebral/métodos , Listas de Espera
3.
Biochem Biophys Res Commun ; 513(1): 119-126, 2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-30935692

RESUMO

Peritoneal fibrosis (PF) is a crucial cause of the loss of peritoneal function in patients with peritoneal dialysis. To better understand the underlying mechanism of PF, we selected AV310809, which is one of the most highly upregulated lncRNA in fibrotic peritoneal tissue, for functional analysis. We used co-expression analysis to explore the potential relationship between AV310809 and coding genes. qPCR, WB and IF were applied to evaluate the expression and localization of AV310809, epithelial markers and proteins involved in the Wnt2/ß-catenin signaling pathway. The interaction between AV310809 and ß-catenin was examined using an RNA pulldown assay. The expression level of AV310809 was upregulated in fibrotic peritoneum and TGF-ß1 induced EMT in HPMCs. Ectopic overexpression of AV310809 promoted EMT and activated the Wnt2/ß-catenin signaling pathway. Furthermore, we demonstrated that AV310809 could interact with ß-catenin and blocking ß-catenin inhibited the augmentation of EMT by AV310809. These findings indicated that AV310809 promoted TGF-ß1-induced EMT in HPMCs through the activation of the Wnt2/ß-catenin signaling pathway, possibly by targeting ß-catenin. We suggest that AV310809 may be a new therapeutic target for the management of peritoneal dialysis-associated PF.

4.
Medicine (Baltimore) ; 98(5): e14196, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30702572

RESUMO

RATIONALE: Spinal epidural abscess (SEA) is a rare condition that shows a high prevalence in immunocompromised patients. The clinical presentation of SEA includes the "classic triad" of pain, fever, and neurological dysfunction. However, these nonspecific features can lead to a high rate of misdiagnosis. SEA may lead to paralysis or even death; thus, prognosis of these patients remains unfavorable. PATIENT CONCERNS: We report a case of a multilevel (T6-T12) SEA in a 22-year-old woman. DIAGNOSIS: The patient was initially diagnosed with spinal tuberculosis at a local hospital based on a history of tuberculosis exposure, as well as radiography and computed tomography. Histopathological examination of the tissue resected during laminectomy confirmed the diagnosis of SEA in this patient. INTERVENTIONS: The patient underwent multilevel laminectomy combined with long-term antibiotic therapy. OUTCOMES: Physical examination performed 16 months postoperatively revealed that superficial and deep sensation was restored to normal levels in the lower extremities with improvement in the patient's motor function (muscle strength 2/5). LESSONS: This case report indicates that whole spine magnetic resonance imaging is warranted in patients with SEA and that prompt surgical intervention is important at symptom onset. Long-term antibiotic therapy is also essential postoperatively.


Assuntos
Doenças Ósseas Infecciosas/diagnóstico , Abscesso Epidural/diagnóstico , Vértebras Torácicas , Antibacterianos/uso terapêutico , Doenças Ósseas Infecciosas/tratamento farmacológico , Doenças Ósseas Infecciosas/cirurgia , Diagnóstico Diferencial , Abscesso Epidural/tratamento farmacológico , Abscesso Epidural/cirurgia , Feminino , Humanos , Laminectomia/métodos , Imagem por Ressonância Magnética , Adulto Jovem
5.
Orthopade ; 48(4): 343-347, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30194630

RESUMO

BACKGROUND: An osteoblastoma is a rare benign bone tumor characterized by formation of osteoid tissue and primitive bone and occurs more often in men than in women. They are often secondary to an osteoid osteoma and can be located at any site on the skeleton. Lesions generally involve the posterior elements of the spine, such as the pedicle and the lamina. CASE PRESENTATION: This study reports the case of a 25-year-old female who suffered from an osteoblastoma of the right sacrum with repeated swelling and pain in the right lumbosacral region for approximately 6 months. Computed tomography (CT) and magnetic resonance imaging (MRI) of the pelvis revealed a segmented, expansive, multiseptate lesion. Resection with wide margins was performed and a huge cavity of approximately 15â€¯× 8â€¯× 4.4 cm in the right sacrum and pelvis was formed after complete curettage of the tumor. The pathological analysis of the resected tissue was consistent with a benign osteoblastoma. A follow-up was performed 2 years later and the patient was eventually relieved of the pain, the mobility of the right leg was improved and the CT scan demonstrated no evidence of recurrence. CONCLUSION: Osteoblastomas most commonly occur in the spine but rarely also in the sacrum. Large core needle biopsies play an important role in the diagnostics. Intralesional surgery can be performed for treatment of osteoblastomas.


Assuntos
Neoplasias Ósseas/diagnóstico , Osteoblastoma/diagnóstico , Sacro , Adulto , Feminino , Humanos , Extremidade Inferior , Recidiva Local de Neoplasia , Osteoma Osteoide
6.
Medicine (Baltimore) ; 97(38): e12496, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30235755

RESUMO

BACKGROUND: Modic changes (MCs) associated with low back pain (LBP) have been assessed in a few studies. It has been documented that patients with LBP have MCs in a specific segment, but the relationship between facet joint or disc degeneration and MCs is still disputed. Thus, we aimed to evaluate the correlation between MC and facet joint or disc degeneration using imaging. METHODS: Imaging data of patients were retrospectively analyzed at the Orthopedic Department of the First Affiliated Hospital of Nanchang from January 2014 to August 2017. MCs, facet joint degeneration, and disc degeneration in L3-S1 were evaluated by lumbar MRI. χ test and contingency correlation coefficient were used for the statistical analyses, and a P value < .05 was considered statistically significant. RESULTS: In the descriptive statistical analysis, MCs were found to have the highest incidence in the L4-5 segment. Type II MCs had a higher incidence than type I and type III MCs regardless of whether they were in the L3-4, L4-5, or L5-S1 segment. On one hand, MCs were more frequently distributed in grades 3, 4, and 5 of the degenerative lumbar discs regardless of whether they were in the L3-4, L4-5, or L5-S1 segment (P < .000, V: contingency coefficient >0); particularly, type II MCs were closely related to lumbar disc degeneration (P < .05, V > 0). On the other hand, MCs were more frequently distributed in grades 1, 2, and 3 of the degenerative lumbar facet regardless of whether they were in the L3-4, L4-5, or L5-S1 segment (P < .05, V > 0). Particularly, type II MCs were frequently distributed in grades 1, 2, and 3 of the facet joint in the L4-5 and L5-S1 segments (P < .05, V > 0). CONCLUSION: MCs are correlated with the grade of lumbar spine degeneration, including lumbar disc and facet joint degeneration. MCs, especially type II, frequently occurred in severe degeneration of the lumbar disc and facet joint. Thus, MC may be one of the manifestations of lumbar disc and facet joint degeneration.


Assuntos
Degeneração do Disco Intervertebral/diagnóstico por imagem , Dor Lombar/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Processamento de Sinais Assistido por Computador , Idoso , Feminino , Humanos , Degeneração do Disco Intervertebral/complicações , Dor Lombar/etiologia , Vértebras Lombares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Articulação Zigapofisária/diagnóstico por imagem
7.
Medicine (Baltimore) ; 97(25): e11210, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29924047

RESUMO

RATIONALE: Osteosarcoma is a rare neoplasm in the lumbar spine. Although osteosarcoma can arise in any portion of the skeleton, it very rarely arises in the spinal canal, which accounts for <0.1% of all cases of adult sarcomas. Here, we describe a case of osteosarcoma arising in the L4-5 spinal canal. PATIENT CONCERNS: The present report describes the case of a 55-year-old female patient with osteosarcoma of the L4-5 spinal canal. DIAGNOSES: The patient was initially diagnosed with lumbar spinal stenosis and underwent lumbar fusion at a local hospital. At the 4-month follow-up, the patient reported a marked increase in numbness and pain in the lumbar region and lower limbs. Based on magnetic resonance imaging, we diagnosed a postoperative infectious lesion of the lumbar spine. INTERVENTIONS: The patient underwent surgery for complete removal of the mass lesion. The mass measured 3 × 2.5 × 0.7 cm in size and was located in the L4-5 spinal canal. OUTCOMES: Based on histological and immunohistochemical findings, the diagnosis of osteosarcoma was confirmed by an expert pathology consultant. The patient then received chemotherapy. Postoperative follow-up at 6 months revealed no evidence of recurrent disease or residual side effects from therapy. LESSONS: Osteosarcoma in the L4-5 spinal canal is extremely rare and very difficult to distinguish histologically from benign nervous and fibrous tissue. This is a very valuable case, which highlights the need for orthopedic surgeons to consider this when diagnosing patients with spinal tumors.


Assuntos
Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/patologia , Osteossarcoma/patologia , Canal Vertebral/patologia , Fusão Vertebral/efeitos adversos , Neoplasias da Coluna Vertebral/patologia , Estenose Espinal/cirurgia , Assistência ao Convalescente , Erros de Diagnóstico , Tratamento Farmacológico/métodos , Feminino , Humanos , Imuno-Histoquímica/métodos , Vértebras Lombares/cirurgia , Região Lombossacral/patologia , Região Lombossacral/cirurgia , Imagem por Ressonância Magnética/métodos , Pessoa de Meia-Idade , Osteossarcoma/metabolismo , Complicações Pós-Operatórias/microbiologia , Fusão Vertebral/métodos , Neoplasias da Coluna Vertebral/cirurgia , Estenose Espinal/diagnóstico , Resultado do Tratamento
8.
Oncol Lett ; 15(3): 3221-3225, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29435061

RESUMO

Although Beclin-1, a well-known key regulator of autophagy, has been demonstrated to serve a function in a number of disorders, including cancer, aging and degenerative diseases, its biological function in Ewing sarcoma (ES) remains unresolved. The objective of the present study was to determine the in vitro effect of Beclin-1 knockdown on the growth and malignant phenotype of ES SK-ES-1 cells, which have increased endogenous expression of Beclin-1 compared with RD-ES cells, and to investigate the underlying molecular mechanism. Cell proliferation, invasion and migration were investigated using CCK-8, Boyden chamber Transwell, and wound healing assays, respectively. Western blot analysis was used to detect expression levels of matrix metalloproteinase (MMP)-2 and MMP-9, which are associated with the malignant phenotype. Beclin-1 knockdown significantly inhibited proliferation, invasion and migration of SK-ES-1 cells. Western blot analysis revealed that Beclin-1 knockdown caused a significant reduction in the expression of MMP-9; no marked changes in MMP-2 expression were observed in the si-Beclin-1 group compared with the control group. The results of the present study suggest that Beclin-1 serves a function in proliferation, tumor progression and inhibition of autophagy in ES, and demonstrates it's potential as a target to increase the efficacy of anticancer agents.

9.
Am J Physiol Renal Physiol ; 314(6): F1087-F1095, 2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29357421

RESUMO

Although epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells was recognized as the key process of peritoneal fibrosis, which is a major cause of peritoneal failure related to peritoneal dialysis (PD), mechanisms underlying these processes remain largely unknown. In this study, we found that miR-200a was significantly downregulated in peritoneal tissues with fibrosis in a rat model of PD. In vitro, transforming growth factor (TGF)-ß1-induced EMT, identified by de novo expression of α-smooth muscle actin and a loss of E-cadherin in human peritoneal mesothelial cells (HPMCs), was associated with downregulation of miR-200a but upregulation of zinc finger E-box-binding homeobox 1/2 (ZEB1/2), suggesting a close link between miR-200a and ZEB1/2 in TGF-ß1-induced EMT. It was further demonstrated that miR-200a was able to bind to the 3'UTR of ZEB1/2, and overexpression of miR-200a blocked TGF-ß1-induced upregulation of ZEB1/2 and, therefore, inhibited EMT and collagen expression. In contrast, overexpression ZEB1/2 blocked miR-200a inhibition of EMT and collagen expression in HMPCs. In conclusion, miR-200a could negatively regulate TGF-ß1-induced EMT by targeting ZEB1/2 in peritoneal mesothelial cells. Blockade of EMT in HPMCS indicates the therapeutic potential of miR-200a as a treatment for peritoneal fibrosis associated with PD.


Assuntos
Células Epiteliais/efeitos dos fármacos , Transição Epitelial-Mesenquimal , MicroRNAs/metabolismo , Fibrose Peritoneal/metabolismo , Peritônio/efeitos dos fármacos , Fator de Crescimento Transformador beta1/farmacologia , Homeobox 2 de Ligação a E-box com Dedos de Zinco/metabolismo , Homeobox 1 de Ligação a E-box em Dedo de Zinco/metabolismo , Regiões 3' não Traduzidas , Animais , Sítios de Ligação , Linhagem Celular , Modelos Animais de Doenças , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Masculino , MicroRNAs/genética , Fibrose Peritoneal/genética , Fibrose Peritoneal/patologia , Peritônio/metabolismo , Peritônio/patologia , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Homeobox 2 de Ligação a E-box com Dedos de Zinco/genética , Homeobox 1 de Ligação a E-box em Dedo de Zinco/genética
10.
BMC Musculoskelet Disord ; 18(1): 427, 2017 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-29100514

RESUMO

BACKGROUND: Osteoarthritis research has been most commonly performed in the setting of the articular cartilage of the knee. To the best of our knowledge, no studies have evaluated the role of adiponectin in osteoarthritis of the lumbar facet joint (FJOA). Therefore, in this study, we explored whether adiponectin was expressed in the lumbar facet joints and evaluated the role of adiponectin in FJOA. METHODS: We enrolled patients who underwent lumbar computed tomography (CT) and magnetic resonance imaging (MRI) at the Orthopedic Department of the First Affiliated Hospital of Nanchang from May 2015 to June 2016. Lumbar facet joints were obtained from 135 patients at the time of lumbar fusion surgery and divided into three groups according to the Weishaupt grade. Cytokine levels in the subchondral bones were evaluated by enzyme-linked immunosorbent assays (ELISAs), and adiponectin levels were determined by immunohistochemistry, western blotting, and quantitative polymerase chain reaction (qPCR). RESULTS: By ELISA, adiponectin levels were examined in the subchondral bone for lumbar facet joint, and adiponectin was found to be negatively correlated with BMI in 52 patients (p < 0.001, r = -0.861). By immunohistochemistry analysis, adiponectin was found to be expressed in the subchondral bone of the lumbar facet, whereas the cartilage area was negative for adiponectin expression. Immunostaining intensity and area was related to the degeneration of the lumbar facet joint, and, in our research, considerably decreased staining intensity and area were observed in more severely degenerated lumbar facet joints. Furthermore, the expression of adiponectin was also reduced in degenerated lumbar facet joints, and the level of decline corresponded to degeneration detected by western blotting and qPCR analysis (n = 27, p < 0.0001). CONCLUSIONS: Adiponectin expression was observed in the subchondral bone of the lumbar facet joint and decreased as the degree of degeneration increased. Thus, the results of this study provide new insights into the relationship between adiponectin and osteoarthritis.


Assuntos
Adiponectina/metabolismo , Vértebras Lombares/metabolismo , Osteoartrite da Coluna Vertebral/metabolismo , Articulação Zigapofisária/metabolismo , Adolescente , Adulto , Idoso , Humanos , Interleucina-1beta/metabolismo , Leptina/metabolismo , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/metabolismo , Adulto Jovem
11.
J Orthop Surg Res ; 12(1): 116, 2017 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-28724387

RESUMO

BACKGROUND: Currently, many scholars are concerned about the treatment of postoperative infection; however, few have completed multivariate analyses to determine factors that contribute to the risk of infection. Therefore, we conducted a multivariate analysis of a retrospectively collected database to analyze the risk factors for acute surgical site infection following lumbar surgery, including fracture fixation, lumbar fusion, and minimally invasive lumbar surgery. METHODS: We retrospectively reviewed data from patients who underwent lumbar surgery between 2014 and 2016, including lumbar fusion, internal fracture fixation, and minimally invasive surgery in our hospital's spinal surgery unit. Patient demographics, procedures, and wound infection rates were analyzed using descriptive statistics, and risk factors were analyzed using logistic regression analyses. RESULTS: Twenty-six patients (2.81%) experienced acute surgical site infection following lumbar surgery in our study. The patients' mean body mass index, smoking history, operative time, blood loss, draining time, and drainage volume in the acute surgical site infection group were significantly different from those in the non-acute surgical site infection group (p < 0.05). Additionally, diabetes mellitus, chronic obstructive pulmonary disease, osteoporosis, preoperative antibiotics, type of disease, and operative type in the acute surgical site infection group were significantly different than those in the non-acute surgical site infection group (p < 0.05). Using binary logistic regression analyses, body mass index, smoking, diabetes mellitus, osteoporosis, preoperative antibiotics, fracture, operative type, operative time, blood loss, and drainage time were independent predictors of acute surgical site infection following lumbar surgery. CONCLUSIONS: In order to reduce the risk of infection following lumbar surgery, patients should be evaluated for the risk factors noted above.


Assuntos
Fixação Interna de Fraturas/efeitos adversos , Vértebras Lombares/cirurgia , Fraturas da Coluna Vertebral/cirurgia , Fusão Vertebral/efeitos adversos , Infecção da Ferida Cirúrgica/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos , Estudos Retrospectivos , Fatores de Risco
12.
Orthopade ; 46(1): 85-89, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27858091

RESUMO

Cryptococcal spine infections are rare infections that are easy to misdiagnose and difficult to cure. Therefore, we report the case of a 25-year-old man who presented with nonspecific spinal lesions at L1 and S1. The patient underwent surgical removal of the lesions, and specimens were submitted for microbial identification, which identified a cryptococcal infection that was susceptible to amphotericin B. The patient exhibited marked improvement after receiving intravenous amphotericin B and remained asymptomatic (no back pain, fever, or other symptoms) at the 3­ and 9­month follow-ups. Similar cases of cryptococcal spine infections are rare, and we believe that our diagnostic findings and treatment experience may help improve the management of this rare disease.


Assuntos
Criptococose/diagnóstico , Criptococose/tratamento farmacológico , Vértebras Lombares/diagnóstico por imagem , Sacro/diagnóstico por imagem , Espondilite/diagnóstico , Espondilite/tratamento farmacológico , Adulto , Criptococose/microbiologia , Diagnóstico Diferencial , Humanos , Vértebras Lombares/microbiologia , Masculino , Sacro/microbiologia , Espondilite/microbiologia , Resultado do Tratamento
13.
Oncol Lett ; 12(5): 3423-3428, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27900015

RESUMO

Osteosarcoma (OS) is a primary malignant tumor of the bone, with a tendency to metastasize early. Despite the advances in treatment options, more than 30% of patients develop distant metastases, and the prognosis of these patients with metastases is extremely poor. Celastrol has been demonstrated to manifest multiple pharmacological activities, including induction of apoptosis in numerous types of cancer cell lines. Our previous studies have also suggested that Celastrol is capable of inducing apoptosis of human osteosarcoma cells via the mitochondrial-dependent pathway. The purpose of this study was to investigate the effects of Celastrol on the migration and invasion of human osteosarcoma U-2OS cells in vitro. Cell migration and invasion were investigated using wound healing and Boyden chamber Transwell assays. We observed that Celastrol suppressed cell invasion and migration in human osteosarcoma U-2OS cells. Furthermore, protein expression levels of phosphorylated phosphatidylinositol 3-kinase (PI3K), Akt, inhibitor of κB kinase α/ß, inhibitor of κB α, nuclear factor-κB (NF-κB subunit p65) and matrix metalloproteinase (MMP)-2 and -9 were measured by western blot analysis. We observed that the PI3K/Akt/NF-κB signaling pathway was inhibited following Celastrol treatment. In addition, the expression levels of MMP-2 and -9 proteins were also reduced significantly following Celastrol treatment. Therefore, we confirmed that Celastrol suppressed osteosarcoma U-2OS cell metastasis via downregulation of the PI3K/Akt/NF-κB signaling pathway in vitro.

14.
Oncol Lett ; 12(4): 2769-2771, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27698855

RESUMO

An aneurysmal bone cyst (ABC) is a rare, non-neoplastic, destructive, hemorrhagic and expansile lesion accounting for 1% of all bone tumors. This type of lesion predominantly affects long bones and vertebrae. ABC of the metatarsal is rare and only a few cases have been reported in the literature to date. The present study reports a rare case of ABC of the third metatarsal occurring in a 27-year-old male patient, who presented with repeated foot swelling that had lasted for ~1 year. Other clinical manifestations included limping, multiple lumps (defined as masses on or below the skin, as detected by imageological diagnosis) and progressively increasing local pain in his right foot. Magnetic resonance imaging of the right metatarsal revealed a segmented, expansile, multiseptated lesion with fluid-fluid levels. An en bloc resection was performed and the defect was replaced with a tricortical iliac autograft. Pathological analysis of the resected tissue suggested ABC. The present study aims to describe a case of ABC of the metatarsal, a condition that often poses a diagnostic challenge, and to underline the importance of radiological and histological examinations for the accuracy of that diagnosis.

15.
Oncol Lett ; 12(3): 1921-1924, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27588141

RESUMO

Pleomorphic rhabdomyosarcoma (PRMS) is a rare type of soft tissue tumor accounting for <2% of all adult sarcomas. The present study describes a case of a 28-year-old male patient with primary PRMS of the right thigh. The patient was initially diagnosed with a schwannoma and underwent conservative therapy at a local hospital. At the 6-month follow-up, the patient reported a marked increase in the size of the mass. Finally, the patient underwent fine-needle aspiration and total tumor resection. The tumor measured 11×9×5 cm3 in size and was located in the vastus intermedius muscle. According to histological and immunohistochemical findings, a diagnosis of PRMS was confirmed by an expert pathology consultant. Postoperative follow-up at 3 months revealed no evidence of recurrent disease or residual side effects from therapy. However, it is imperative that such cases are closely monitored following surgery, in order to evaluate the long-term efficacy of the procedure, since misdiagnosis may increase the risk of recurrence and metastasis. The present case is noteworthy due to the misdiagnosis of PRMS, the large size of the mass and the young age of the patient.

16.
Chin Med J (Engl) ; 129(12): 1439-46, 2016 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-27270539

RESUMO

BACKGROUND: Brain acid soluble protein 1 (BASP1) is identified as a novel potential tumor suppressor in several cancers. However, its role in thyroid cancer has not been investigated yet. In the present study, the antitumor activities of BASP1 against the growth and migration of thyroid cancer cells were evaluated. METHODS: BASP1 expression in thyroid cancer tissues and normal tissues were examined by immunohistochemical staining and the association between its expression and prognosis was analyzed. pcDNA-BASP1 carrying full length of BASP1 cDNA was constructed to restore the expression of BASP1 in thyroid cancer cell lines (BHT-101 and KMH-2). The cell proliferation in vitro and in vivo was evaluated by WST-1 assay and xenograft tumor models, respectively. Cell cycle distribution after transfection was analyzed using flow cytometry. Cell apoptosis after transfection was examined by annexin V/propidium iodide assay. The migration was examined using transwell assay. RESULTS: BASP1 expression was abundant in normal tissues while it is significantly decreased in cancer tissues (P = 0.000). pcDNA-BASP1 restored the expression of BASP1 and significantly inhibited the growth of BHT-101 and KMH-2 cells as well as xenograft tumors in nude mice (P = 0.000). pcDNA-BASP1 induced G1 arrest and apoptosis in BHT-101 and KMH-2 cells. In addition, pcDNA-BASP1 significantly inhibited the cell migration. CONCLUSIONS: Downregulation of BASP1 expression may play a role in the tumorigenesis of thyroid cancer. Restoration of BASP1 expression exerted extensive antitumor activities against growth and migration of thyroid cancer cells, which suggested that BASP1 gene might act as a potential therapeutic agent for the treatment of thyroid cancer.


Assuntos
Proteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Proteínas Repressoras/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Idoso , Animais , Apoptose/genética , Apoptose/fisiologia , Proteínas de Ligação a Calmodulina/genética , Proteínas de Ligação a Calmodulina/metabolismo , Ciclo Celular/genética , Ciclo Celular/fisiologia , Linhagem Celular Tumoral , Movimento Celular/genética , Movimento Celular/fisiologia , Proliferação de Células/genética , Proliferação de Células/fisiologia , Proteínas do Citoesqueleto/genética , Proteínas do Citoesqueleto/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Regulação Neoplásica da Expressão Gênica/fisiologia , Humanos , Masculino , Proteínas de Membrana/genética , Camundongos , Camundongos Nus , Pessoa de Meia-Idade , Proteínas do Tecido Nervoso/genética , Proteínas Repressoras/genética , Neoplasias da Glândula Tireoide/patologia , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Oncol Lett ; 11(2): 1481-1485, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26893764

RESUMO

The patella is an unusual location for primary and metastatic bone tumors to develop. The most frequently encountered primary osteolytic lesions at the patella include giant cell tumors of the bone (GCT), chondroblastoma and aneurysmal bone cysts (ABC). However, the presentation of an ABC originating secondary to a GCT at the patella is rare. The present study describes such a case in a 46-year-old female. The differential diagnosis of the condition was extensive. The patient underwent curettage and the addition of bone cement to fill the defect. Pathological analysis of the resected tissue demonstrated that the lesion was consistent with an ABC forming secondary to a GCT. A 3-month follow-up was completed subsequent to the surgery, with a computed tomography scan demonstrating no evidence of recurrence. However, frequent and continuous observations of the patient following diagnosis are planned in order to evaluate the long-term efficacy of the surgical treatment. To the best of our knowledge, the present study describes the third reported case in the literature of this rare, double synchronous, benign tumor located at the patella.

18.
Oncol Lett ; 12(6): 4749-4755, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28105184

RESUMO

Increasing evidence has revealed a correlation between chronic inflammation and gallbladder cancer (GBC). However, the underlying molecular mechanisms remain to be elucidated. In the present study, secretion of interleukin (IL)-1ß was examined in tissues of GBC, chronic cholecystitis and normal gallbladder, as well as in the supernatant of GBC-SD, SGC996 and HIBEpiC cells. The effect of IL-1ß on the proliferation and migration of GBC cell lines was also evaluated. In addition, the role of Twist in IL-1ß-induced proliferation of GBC cells was also studied. It was observed that the level of IL-1ß protein in normal gallbladder tissue was low, while it was significantly increased in GBC and chronic cholecystitis tissues. The level of IL-1ß protein and mRNA in GBC-SD and SGC996 cells was markedly higher than those in HIBEpiC cells. Exogenous IL-1ß promoted the proliferation of GBC-SD and SGC996 cells in vitro and in vivo, and also promoted migration in vitro. The level of Twist protein was significantly increased following treatment with exogenous IL-1ß. In addition, gene silencing of Twist blocked IL-1ß-induced proliferation and migration of GBC-SD and SGC996 cells. Taken together, these results indicate that IL-1ß promotes proliferation and migration of GBC cells via Twist activation.

19.
Surg Laparosc Endosc Percutan Tech ; 25(5): 399-402, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26429049

RESUMO

OBJECTIVE: To evaluate the efficacy of laparoscopy combined with transanal endoscopic microsurgery (TEM) in the treatment of rectal cancer. METHODS: A total of 60 rectal cancer patients who underwent radical resection from December 2009 to December 2013 were enrolled in this study. All patients were randomly divided to receive either laparoscopic surgery (LA group) or laparoscopy combined with TEM (LT group). Demographics including age and sex, and tumor characteristics including tumor size, distance from anal verge, and preoperative staging were all recorded. The operation time, hospital stay, cases with intraoperative blood transfusion, the number of resected lymph node, postoperative out-of-bed activity, passage of gas by anus, fasting time, and postoperative complications were assessed. RESULTS: Compared with the LA group, patients in the LT group had shorter operation time (118.5±22.0 vs. 138.1±23.8 min, P=0.002), earlier out-of-bed activity (60.4±19.2 vs. 83.6±9.6 h, P=0.001) and passage of gas by anus (81.4±5.4 vs. 86.2±8.7 h, P=0.013), and shorter hospital stay (8.0±2.8 vs. 11.0±3.5 d, P=0.001). Patients were followed up for a median time of 28 months (range, 3 to 48 mo). No local recurrence and distant metastasis occurred in all cases. CONCLUSIONS: Laparoscopy combined with TEM is a feasible and effective treatment for radical excision of middle-upper rectal cancer due to the advantages of lower morbidity and earlier recovery.


Assuntos
Adenocarcinoma/cirurgia , Colectomia/métodos , Laparoscopia/métodos , Neoplasias Retais/cirurgia , Reto/cirurgia , Microcirurgia Endoscópica Transanal/métodos , Adenocarcinoma/diagnóstico , Idoso , Feminino , Humanos , Tempo de Internação/tendências , Masculino , Estadiamento de Neoplasias , Duração da Cirurgia , Proctoscopia , Estudos Prospectivos , Neoplasias Retais/diagnóstico , Reto/patologia , Método Simples-Cego , Resultado do Tratamento
20.
Oncol Rep ; 34(3): 1129-36, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26165547

RESUMO

Celastrol is an active compound extracted from the root bark of Triptergium wilfordii Hook F., also known as ̔Thunder of God Vine̓. It is a well-known Chinese medicinal herb that was found to inhibit tumor cell growth and promote apoptosis in several tumor cell lines. However, research into its effects on osteosarcoma cell apoptosis is still extremely limited. The present study was undertaken to determine the effect of celastrol on viability and apoptosis of osteosarcoma cells and furthermore, to illuminate the molecular mechanism of celastrol-induced osteosarcoma cell apoptosis. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay was used to evaluate the viability of the cells following treatment with celastrol. The effect of celastrol on the apoptotic rate of the cells was evaluated by flow cytometry using Annexin V-PE/7-AAD staining assay. Fluorescence microscopy was used to detect the morphological changes in the human osteosarcoma U-2OS cell lines. The expression of Bcl-2 family proteins, caspase-3, caspase-8, caspase-9, cytochrome c and PARP was measured by western blotting. We found that celastrol significantly inhibited the growth of osteosarcoma cells in a dose-dependent manner, particularly U-2OS cells. Furthermore, we observed that celastrol upregulated the expression of the pro-apoptotic proteins Bax and cytochrome c and altered the ratio of Bax/Bcl-2, and triggered the mitochondrial apoptotic pathway, resulting in caspase-3 and -9 activation and PARP cleavage. To conclude, the results indicate that celastrol inhibits the proliferation of human osteosarcoma cancer cells by inducing apoptosis via the mitochondrial-dependent pathway.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Osteossarcoma/patologia , Triterpenos/farmacologia , Western Blotting , Neoplasias Ósseas/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citometria de Fluxo , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos
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