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1.
J Dig Dis ; 20(10): 547-556, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31448533

RESUMO

OBJECTIVE: Lactulose is effective in the treatment and prevention of overt hepatic encephalopathy (OHE), but there are limited data on its use on microbiota in relations to minimal hepatic encephalopathy (MHE) recovery. The present study aimed to assess the efficacy of lactulose in recovery of MHE in aspects of cognitive function, quality of life, and impact on intestinal microbiota. METHODS: This multicenter, open-label randomized controlled trial was conducted in 11 teaching hospitals in China. Participants were randomly allocated on a 2:1 basis to receive lactulose (Gp-L) or no therapy as control (Gp-NL) for 60 days. The primary endpoint was the MHE reversal rate. Gut microbiota were compared between MHE patients and healthy volunteers, as well as lactulose-responders and non-responders. RESULTS: A total of 98 cirrhotic patients were included in the study, with 31 patients in the Gp-NL group and 67 patients in the Gp-L group. At day 60, the MHE reversal rate in Gp-L (64.18%) was significantly higher than that in Gp-NL (22.58%) (P = .0002) with a relative risk of 0.46 (95% confidence interval 0.32-0.67). Number needed to treat was 2.4. Further, there was significantly more improvement in physical functioning in Gp-L (4.62 ± 6.16) than in Gp-NL (1.50 ± 5.34) (P = .0212). Proteobacteria was significantly higher in MHE patients compared with healthy volunteers (12.27% vs 4.65%, P < .05). Significant differences were found between lactulose responders and non-responders in Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. CONCLUSIONS: Treatment with lactulose significantly improves MHE recovery rate, and gut microbiota change in MHE patients can modulate the effectiveness of this therapy. Chinese Clinical Trial Register (ChiCTR) (ID: ChiCTR-TRC-12002342).

2.
Medicine (Baltimore) ; 98(7): e14368, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30762736

RESUMO

RATIONALE: Hepatocellular carcinoma (HCC) is the fifth most common cancer and third leading cause of cancer-related deaths worldwide. Nasopharyngeal metastasis of hepatocellular carcinoma is very rare. This is the first report of posttransplantation nasopharyngeal metastasis. PATIENT CONCERNS: A 45-year-old man with a history of hepatitis B related hepatocellular carcinoma (HCC) in the right segment of the liver received an orthotopic liver transplant. Two year after the transplantation, he suffered from severe headache, and head contrast enhanced CT scans did not show clues for brain or skull metastasis. Then he developed hoarseness and dysphagia. DIAGNOSIS: The nasopharyngeal cancer was confirmed to be metastatic tumor from liver histologically according to biopsy. INTERVENTIONS: This patient underwent radiotherapy (RT) of the metastatic nasopharyngeal tumor, and there was significant symptomatic relief. OUTCOMES: The patient died 3 months after nasopharyngeal metastasis was diagnosed. LESSONS: Recurrence of hepatocellular carcinoma with metastasis of nasopharyngeal carcinoma after liver transplantation is rare, but the prognosis is very poor. Close follow-up of patients should be paid attention to prevent the occurrence of such diseases.


Assuntos
Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , Neoplasias Nasofaríngeas/secundário , Carcinoma Hepatocelular/complicações , Carcinoma Hepatocelular/cirurgia , Hepatite B/complicações , Humanos , Neoplasias Hepáticas/complicações , Neoplasias Hepáticas/cirurgia , Transplante de Fígado , Masculino , Pessoa de Meia-Idade , Neoplasias Nasofaríngeas/radioterapia
3.
Cell Death Dis ; 9(5): 526, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29743561

RESUMO

Somatic cells acquire stem cell-like properties during cancerous transformation; however, mechanisms through which committed cells develop stemness and malignancy remain largely unknown. Here we uncovered upregulated stem cell program in leukaemic lymphoblasts of patients with IKZF1 alterations by analysing the archived gene-expression profiling datasets. We then used a frequent IKZF1 deletion, IK6, as a model via transduction into human primitive haematopoietic cells, followed by xenotransplantation in mice. Immunophenotypically defined stem, pro-B, and immature/mature (IM/M)-B cells were collected from primary recipients for functional assay and transcriptome profiling. Successful reconstitution in secondary recipient mice revealed the stemness of IK6+ pro-B and IM/M-B cells. Upregulated stemness and malignancy programs in IK6+ cells confirmed IK6 effects. Interestingly, these programs corresponded to distinct canonical pathways. Remarkably, the pathway profile mapped in the modelled cells well mirrored that in patients' leukaemic cells; therefore, our study provides a seminal insight into the cancerous reprogramming of somatic cells.


Assuntos
Fator de Transcrição Ikaros , Leucemia , Linfócitos/metabolismo , Proteínas de Neoplasias , Células-Tronco Neoplásicas/metabolismo , Animais , Humanos , Fator de Transcrição Ikaros/genética , Fator de Transcrição Ikaros/metabolismo , Leucemia/genética , Leucemia/metabolismo , Leucemia/patologia , Linfócitos/patologia , Camundongos Endogâmicos NOD , Camundongos SCID , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Células-Tronco Neoplásicas/patologia
4.
Oncotarget ; 8(38): 63360-63369, 2017 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-28968996

RESUMO

Anaphase promoting complex/cyclosome (APC/C) is essential for cell cycle progression. Recently, its non-mitotic functions were also reported but less studied in several tissues including hematopoietic cells. Here, we developed an inducible Anapc2 (a core subunit of APC/C) knockout mice. The animals displayed a fatal bone marrow failure within 7 days after knockout induction. Their hematopoietic stem and progenitor cells (HSPCs) demonstrated a sharp decline and could form little colony. Further, the results of BrdU label-retaining cell assay showed that the dormant HPSCs lost rapidly. Analysis of cell cycle regulators, Skp2, P27, Cdk2, and Cyclin E1, suggested that these quiescent stem cells underwent a shift from quiescence to mitosis followed by apoptosis. We next detected Anapc2-expression in the CD34+ HSPCs of patients with aplastic anemia. CD34+ cells were markedly decreased in the bone marrow and Anapc2-expression in the residual CD34+ cells was undetectable, suggesting that APC/C was deficient and might have a relationship with the pathogenesis of aplastic anemia.

5.
Int J Hematol ; 105(5): 558-565, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28176227

RESUMO

Increasing studies have revealed that the interaction between malignant cells and the microenvironment (so called niche) in the bone marrow can influence the development and progression of the hematopoietic malignancies. Here, we reviewed the current findings in the field, focusing the niche alterations in promoting the emergency of malignancies, in interfering with the blood reconstitution of normal hematopoietic stem and progenitor cells, and in protecting leukemic stem cells from therapy which causes disease relapse. We made efforts to discuss these aspects in view of a kind of disturbance of the microecosystem within BM and thus proposed some new concepts in therapeutics of blood malignancies.


Assuntos
Medula Óssea , Neoplasias Hematológicas/patologia , Neoplasias Hematológicas/terapia , Células-Tronco Hematopoéticas , Microambiente Tumoral , Humanos
6.
Oncotarget ; 8(65): 108981-108988, 2017 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-29312584

RESUMO

The fetal liver (FL) is a source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, whether FL-HSPCs collected at distinct developmental stages reconstitute similarly or differently in the recipient bone marrow (BM) remains undetermined. We examined this problem in a congeneic mouse transplantation model. We first analyzed the lineage components of FL from 12.5 days post-fertilization (dpf) to 18.5 dpf. The myeloid and lymphoid cells were dynamic in absolute number and ratio. The largest difference was between 12.5 and 16.5 dpf. The FL-HSPCs (Lin-CD150+CD48-) at these two time points were then respectively transplanted into the recipients. The difference in lineage reconstitution was undetectable at week 4 or 6 post-transplantation and afterward, indicating that the BM environment assimilated the transplanted cells. Profiling lineage-regulation genes of input and output HSPCs showed that the expression levels were much different in the former and almost the same in the engrafted HSPCs. Therefore, the recipient BM microenvironment could determine the developmental lineage-trends of FL-HSPCs.

7.
Zhonghua Nan Ke Xue ; 23(12): 1085-1088, 2017 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29738178

RESUMO

Objective: To compare thulium laser vaporization of the prostate (TLVP) and transurethral resection of the prostate (TURP) in the treatment of benign prostate hyperplasia (BPH) analyze the risk factors for postoperative urethral stricture. METHODS: From June 2015 to June 2016, 210 BPH patients in our hospital underwent TURP (n = 126) or TLVP (n = 84). We followed up the patients for 6 months, compared the effects of the two surgical strategies and analyzed the risk factors for postoperative urethral stricture by multivariate logistic regression analysis. RESULTS: Compared with TURP, TLVP achieved significantly shorter time of operation (ï¼»78.6 ± 27.5ï¼½ vs ï¼»53.2 ± 21.6ï¼½ min, P <0.01), postoperative bladder irrigation (ï¼»31.5 ± 2.9ï¼½ vs ï¼»26.1 ± 3.7ï¼½ h, P <0.01), urethral catheterization (ï¼»5.3 ± 1.7ï¼½ vs ï¼»3.7 ± 1.5ï¼½ d, P <0.01) and postoperative hospitalization (ï¼»7.9 ± 2.1ï¼½ vs ï¼»5.5 ± 1.4ï¼½ d, P <0.01) as well as lower urinary leukocyte count at 6 months after surgery (ï¼»32.1 ± 12.6ï¼½ vs ï¼»24.9 ± 11.7ï¼½ /µl, P <0.01) and incidence rate of postoperative complications (11.9% ï¼»15/126ï¼½ vs 3.6% ï¼»3/84ï¼½, P <0.05), particularly that of urethral stricture (7.9% ï¼»10/126ï¼½ vs 1.2% ï¼»1/84ï¼½, P <0.05). Logistic regression analysis showed that the preoperative urinary leukocyte count, postoperative urethral catheterization time, and surgical method were independent risk factors for postoperative urethral stricture. CONCLUSIONS: TLVP, in comparison with TURP, has the advantages of definite effect, fast recovery, high safety and low incidence of postoperative urethral stricture. The main risk factors for postoperative urethral stricture include preoperative urinary tract infection, postoperative urethral catheterization time and surgical method.


Assuntos
Terapia a Laser/efeitos adversos , Complicações Pós-Operatórias/etiologia , Hiperplasia Prostática/cirurgia , Túlio/uso terapêutico , Ressecção Transuretral da Próstata/efeitos adversos , Estreitamento Uretral/etiologia , Humanos , Terapia a Laser/métodos , Masculino , Duração da Cirurgia , Qualidade de Vida , Análise de Regressão , Fatores de Risco , Resultado do Tratamento , Cateterismo Urinário , Infecções Urinárias/complicações
8.
Asian J Androl ; 17(1): 124-34, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25337836

RESUMO

We performed a meta-analysis to compare treatment with a combination of solifenacin plus tamsulosin oral controlled absorption system (TOCAS) with placebo or TOCAS monotherapy. The aim of the meta-analysis was to clarify the efficacy and safety of the combination treatments method for lower urinary tract symptoms (LUTS). We searched for trials of men with LUTS that were randomized to combination treatment compared with TOCAS monotherapy or placebo. We pooled data from three placebo-controlled trials meeting inclusion criteria. Primary outcomes of interest included changes in International Prostate Symptom Score (IPSS) and urinary frequency. We also assessed postvoid residual, maximum urinary flow rate, incidence of urinary retention (UR), adverse events. Data were pooled using random or fixed effect models for continuous outcomes and the Mantel-Haenszel method to generate risk ratio. Reductions in IPSS storage subscore and total urgency and frequency score (TUFS) were observed with solifenacin 6 mg plus TOCAS compared with placebo (P< 0.0001 and P< 0.0001, respectively). Reductions in IPSS storage subscore and TUFS were observed with solifenacin 9 mg plus TOCAS compared with placebo (P = 0.003 and P= 0.0006, respectively). Reductions in TUFS was observed with solifenacin 6 mg plus TOCAS compared with TOCAS (P = 0.01). Both combination treatments were well tolerated, with low incidence of UR. Solifenacin 6 mg plus TOCAS significantly improved total IPSS, storage and voiding symptoms compared with placebo. Solifenacin 6 mg plus TOCAS also improved storage symptoms compared with TOCAS alone. There was no additional benefit of solifenacin 9 mg compared with 6 mg when used in combination with TOCAS.


Assuntos
Sintomas do Trato Urinário Inferior/tratamento farmacológico , Succinato de Solifenacina/efeitos adversos , Succinato de Solifenacina/uso terapêutico , Sulfonamidas/efeitos adversos , Sulfonamidas/uso terapêutico , Administração Oral , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Humanos , Incidência , Sintomas do Trato Urinário Inferior/fisiopatologia , Masculino , Succinato de Solifenacina/administração & dosagem , Sulfonamidas/administração & dosagem , Tansulosina , Resultado do Tratamento , Retenção Urinária/epidemiologia , Retenção Urinária/fisiopatologia , Micção/fisiologia , Agentes Urológicos/administração & dosagem , Agentes Urológicos/efeitos adversos , Agentes Urológicos/uso terapêutico
9.
Tumour Biol ; 35(8): 8209-16, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24850174

RESUMO

We conducted the present meta-analysis of relevant cohort studies to evaluate whether promoter methylation of the high in normal-1 (HIN-1) gene contributes to breast cancer. The MEDLINE (1966 ~ 2013), Cochrane Library (Issue 12, 2013), EMBASE (1980 ~ 2013), CINAHL (1982 ~ 2013), Web of Science (1945 ~ 2013), and Chinese Biomedical (CBM) (1982 ~ 2013) databases were searched without any language restrictions. Meta-analyses were conducted using Stata software (version 12.0; Stata Corporation, College Station, TX, USA). Crude odds ratios (ORs) with their 95 % confidence interval (CI) were calculated. Nine clinical cohort studies that enrolled a total of 693 breast cancer patients were included in the meta-analysis. The results of our meta-analysis demonstrated that HIN-1 methylation frequency in cancer tissue was significantly higher than that of normal and benign tissues (cancer tissue vs. normal tissue: OR = 52.60, 95 % CI = 33.77 ~ 81.92, P < 0.001; cancer tissue vs. benign tissue: OR = 2.38, 95 % CI = 1.53 ~ 3.70, P < 0.001; respectively). Ethnicity-stratified analysis indicated that HIN-1 promoter methylation was correlated with the pathogenesis of breast cancer among both Asians and Caucasians (all P < 0.05). Our findings provide empirical evidence that aberrant HIN-1 promoter methylation may contribute to the pathogenesis of breast cancer. Thus, aberrant HIN-1 promoter methylation could be an independent and important biomarker used in predicting the prognosis and progression of breast cancer.


Assuntos
Neoplasias da Mama/genética , Citocinas/genética , Metilação de DNA , Predisposição Genética para Doença , Regiões Promotoras Genéticas , Proteínas Supressoras de Tumor/genética , Neoplasias da Mama/etiologia , Feminino , Humanos
10.
Zhonghua Yi Xue Za Zhi ; 93(25): 1962-4, 2013 Jul 02.
Artigo em Chinês | MEDLINE | ID: mdl-24169244

RESUMO

OBJECTIVE: To analyze the results of detection on respiratory virus of influenza-like illness ( ILI ) in Beijing from June 2010 to February 2012 and understand the virus spectrum of adult influenza-like fever. METHODS: A total of 502 swabs were collected and 279 throat swabs tested for 12 respiratory viruses with multiplex reverse transcription-polymerase chain reaction (RT-PCR). And 413 swabs were tested for pH1N1 by virus isolation influenza viruses. And the data were statistically analyzed. RESULTS: One or two viruses were detected in 26.9% (75/279) of the samples. Influenza A virus (FLU-A) accounted for 85.3% of positive samples and 22.9% (64/279) of ILI tested. The positive rate of other viruses was less than 3.0 %. The positive rates among the following subtypes were: 2.7% (11/413) for pH1N1, 2.4% (10/413) for H3 and 6.5% (27/413) for FLU-B. FLU-A was the predominant virus during the 2010-2011 influenza season and the positive rate peaked in January 2011 in Beijing and north China. FLU-B was the primary virus during the 2011-2012 influenza season and the positive rate peaked in January and February 2012. There was a significant reduction in the incidence of ILI in 2010 and 2011 when compared with 2009. During the 2009-2012 influenza seasons, the incidence peaked in December 2009, January 2011 and January and February 2012 in Beijing. CONCLUSIONS: Exposure to pH1N1 had no impact on typical influenza seasonal peaks. Influenza virus was the predominant virus of adult influenza-like fever cases after the pandemic period of influenza A (H1N1) 2009 and the positive rate peaked in January and February during the 2009-2012 influenza seasons.


Assuntos
Febre/virologia , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Influenza Humana/epidemiologia , Influenza Humana/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Humanos , Pessoa de Meia-Idade , Adulto Jovem
11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2702-5, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23285869

RESUMO

In the present paper, taking Tiegunyin tea as an example, surface enhanced Raman scattering (SERS) spectrum of tea was obtained, the effects of different active substrates were explored from the aspects of enhanced factor, background noise, repeatability, and signal to noise ratio (SNR), while the impact of adsorption time on the measurement was also discussed. The results show that it is feasible to obtain the SERS spectrum of tea, and Raman signal of SERS spectrum was greatly enhanced compared with that of regular Raman spectrum. The active substrate of silver colloid prepared by the reduction of silver nitrate and trisodium citrate has a better enhanced effect, while different adsorption times have no direct influences on the SERS measurement. The method based on SERS firstly proposed in this paper may provide an alternative method for the discriminant analysis and quality identification of tea.


Assuntos
Análise Espectral Raman , Chá/química , Citratos/química , Oxirredução , Nitrato de Prata/química , Propriedades de Superfície
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(3): 809-11, 2010 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-20496715

RESUMO

A flow injection-hydride generation-atomic absorption spectrometric method for the determination of selenium in poultry eggs was developed. Various testing conditions and effect factors for the determination of selenium were studied. The authors also discussed the content of selenium in poultry eggs and the application of scientific feed method in improving selenium content in poultry eggs. The detection limit for selenium was 0.25 microg x L(-1) under optimum conditions. The linear range for selenium was 0.25-60 microg x L(-1). The relative standard deviation was less than 2.5% and the recovery was 95%-108%. This method overcame the problem of severe matrix interferences of graphite furnace atomic absorption spectrometry, in which matrix modifier must be added to eliminate matrix interference by raising ashing temperature. Additionally this method overcame the shortcoming of slow analysis procedure, complex operation and error caused by manual injection in traditional interval hydride generation-atomic absorption spectrometry. It is a simple, rapid, highly sensitive and automatic method and has been applied to the determination of selenium in poultry eggs with satisfactory results.


Assuntos
Ovos/análise , Selênio/análise , Espectrofotometria Atômica , Animais , Grafite , Limite de Detecção , Aves Domésticas , Temperatura Ambiente
13.
J Cancer Res Clin Oncol ; 135(8): 1033-9, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19132389

RESUMO

PURPOSE: Human pepsinogen C (PGC) is an aspartic protease produced specifically by the gastric mucosa, and is considered as a mature marker of gastric epithelium. This study examined the contributions of PGC polymorphisms and the Helicobacter pylori (H. pylori) infection to the risk of gastric cancer (GC), and its precancerous conditions in a Northeast Chinese population. METHODS: The PGC insertion/deletion polymorphism was evaluated by polymerase chain reaction analysis, followed by direct DNA sequencing in 564 cases of GC, atrophic gastritis (AG), gastric ulcer (GU) and superficial gastritis (as control). All cases were frequency-matched 1:1 by gender and age (+/-5). H. pylori infection was identified by serum anti-H. pylori IgG measurement through enzyme-linked immunosorbent assay. RESULTS: Patients with a homozygous PGC allele 1 genotype had a significant risk of AG [adjusted odds ratio (OR) 3.11; 95% confidence interval (CI) 1.44-6.71] or of GC (OR 3.00; 95% CI 1.38-6.51), and a significantly elevated risk of intestinal metaplasia (OR 1.90, 95% CI 1.11-3.27). PGC polymorphism with H. pylori infection increased risk of GU (OR 8.69; 95% CI 1.01-74.69), and AG (OR 11.12; 95% CI 1.37-90.84) or GC (OR 10.61; 95% CI 1.28-87.79) in a super-multiplicative manner. The S value was 5.40, 6.48 and 4.34; and the AP value was 72.09, 7.00 and 69.69%, respectively. CONCLUSIONS: The PGC gene polymorphism increases an individual's susceptibility to GC and its precancerous conditions. Moreover, the PGC gene polymorphism shows a positive link to H. pylori infection in the development of GC.


Assuntos
Predisposição Genética para Doença/genética , Pepsinogênio C/genética , Polimorfismo Genético , Lesões Pré-Cancerosas/genética , Neoplasias Gástricas/genética , Grupo com Ancestrais do Continente Asiático/genética , Sequência de Bases , China , Ensaio de Imunoadsorção Enzimática , Gastrite Atrófica/genética , Genótipo , Infecções por Helicobacter/genética , Helicobacter pylori/patogenicidade , Humanos , Dados de Sequência Molecular , Lesões Pré-Cancerosas/etnologia , Neoplasias Gástricas/etnologia , Úlcera Gástrica/genética
14.
Zhonghua Liu Xing Bing Xue Za Zhi ; 29(12): 1225-9, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19173969

RESUMO

OBJECTIVE: To understand the biochemical characteristics and 16S rDNA genetic sequence evolution of strains isolated from diarrhea specimens so as to provide basis for classification and identification of Klebsiella pneumoniae. METHODS: Specimens were cultured using MacConkey and SS medium. All isolates were identified as K. pneumoniae by automated biochemical tests. DNA was extracted, 1500 bp fragments of the 16S rDNA gene were by amplified PCR and sequenced with K. pneumoniae 16S rDNA primer, after being cut. Fragments of 1000 bp overlapping sequences were analyzed by Blast to confirm the identity of the isolates. A phylogenetic tree was constructed by PHYLIP process to analyze the 16S rDNA sequence of the isolated strain with other relative bacteria species in the GenBank databases. RESULTS: Among 113 specimens of infectious diarrhea, 25 K. pneumoniae strains were identified by biochemical tests, of which 21 subsp. pneumoniae and 4 subsp. ozaenae, no subsp. of rhinoscleroma were isolated. Strains of subsp. pneumoniae were found having nature of resistance. All isolates were resistant to penicillin G and susceptible to polymyxin with some strains were resistant to Nitrofurantoin, Cephalothin, Kanamycin, Tobramycin. After searching in GenBank of 16S rDNA, strains biochemical identified as subsp. ozaenae shared high similarity with Salmonella strains and other intestinal bacteria. 16S rDNA phylogenetic analysis could be used to confirm subsp. pneumoniae, but could not separate other subspecies of K. pneumoniae completely. CONCLUSION: 16S rDNA phylogenetic analysis useful in identifying and classifying K. pneumoniae.


Assuntos
Diarreia/microbiologia , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Filogenia , RNA Ribossômico 16S/genética , Primers do DNA , DNA Bacteriano/genética , Evolução Molecular , Humanos , Klebsiella pneumoniae/classificação , Testes de Sensibilidade Microbiana , RNA Bacteriano
15.
J Urol ; 176(3): 1077-80, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16890694

RESUMO

PURPOSE: We evaluated the clinical value of retroperitoneal laparoscopic dismembered pyeloplasty for ureteropelvic junction obstruction compared with open surgery. MATERIALS AND METHODS: The clinical data of 56 patients who underwent retroperitoneal laparoscopic dismembered pyeloplasty were retrospectively compared with those of 40 patients who underwent open dismembered pyeloplasty through a retroperitoneal flank approach. The Student t test, Pearson chi-square test and Mann-Whitney rank sum test were applied for statistical analysis as appropriate. RESULTS: Patient demographic data were similar between the 2 groups. In the laparoscopic group operative time (80 vs 120 minutes), estimated blood loss (10 vs 150 ml), recovery of intestinal function (1 vs 2 days), analgesic requirements (diclofenac sodium suppository) (75 vs 150 mg), incision length (3.5 vs 21 cm) and postoperative hospital stay (7 vs 9 days) were better than in the open group (p <0.001 for all). No intraoperative complications occurred in either group. The incidence of postoperative complications (2 of 56, 3.6% vs 3 of 40, 7.5%, p = 0.729) and success rates (55 of 56, 98.2% vs 39 of 40, 97.5%, p = 0.058) were equivalent in the 2 groups. CONCLUSIONS: Retroperitoneal laparoscopic dismembered pyeloplasty is a minimally invasive, safe and effective therapy for ureteropelvic junction obstruction with low morbidity, shorter convalescence and excellent outcomes, and can be accomplished reasonably quickly in experienced hands.


Assuntos
Pelve Renal/cirurgia , Laparoscopia , Obstrução Ureteral/cirurgia , Adulto , Feminino , Humanos , Masculino , Espaço Retroperitoneal , Estudos Retrospectivos , Procedimentos Cirúrgicos Urológicos/métodos
16.
Zhonghua Zhong Liu Za Zhi ; 28(2): 120-2, 2006 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-16750016

RESUMO

OBJECTIVE: To study the expression and significance of interleukin-8 (IL-8), cyclooxygenase-2 (COX-2) and trefoil family factor 1 (TFF1) in the remnant stomach mucosa. METHODS: Patients after gastrectomy were examined by upper gastrointestinal endoscopy. Biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to be assessed for Hp (by H.E. and Giemsa staining) and conduct real-time semi-quantitative PCR. mRNA was extracted from the biopsy specimens to determine the IL-8, COX-2 and TFF1 gene mRNA levels by real-time PCR method. RESULTS: In the stoma, COX-2 level in Hp-positive patients was significantly higher than that in Hp-negative patients, but the difference of IL-8 levels between them was not significant. In the corpus, IL-8 and COX-2 levels in Hp-positive patients were significantly higher than those in Hp-negative patients. In Hp-negative patients, IL-8 and COX-2 levels in the stoma were significantly higher in B II anastomosis than in B I anastomosis cases; COX-2 level in the stoma was significantly higher in B II anastomosis than in B I anastomosis cases, but the difference of IL-8 levels between them was not significant. TFF1 level in the remnant stomach mucosa showed no significant difference between Hp-positive and Hp-negative patients. CONCLUSION: Hp infection and bile reflux are important risk factors for the secondary stomach carcinogenesis. Expression of IL-8 and COX-2 in the remnant stomach mucosa is related to the risk of secondary stomach carcinogenesis. The relationship between the TFF1 expression and secondary stomach carcinogenesis in the remnant stomach mucosa is still unclear and should further be studied.


Assuntos
Ciclo-Oxigenase 2/biossíntese , Coto Gástrico , Interleucina-8/biossíntese , Neoplasias Gástricas/metabolismo , Proteínas Supressoras de Tumor/biossíntese , Adulto , Idoso , Idoso de 80 Anos ou mais , Ciclo-Oxigenase 2/genética , Feminino , Gastrectomia , Mucosa Gástrica/metabolismo , Infecções por Helicobacter/metabolismo , Helicobacter pylori , Humanos , Interleucina-8/genética , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Fatores de Risco , Neoplasias Gástricas/microbiologia , Neoplasias Gástricas/cirurgia , Fator Trefoil-1 , Proteínas Supressoras de Tumor/genética
17.
Zhonghua Yi Xue Za Zhi ; 86(43): 3059-63, 2006 Nov 21.
Artigo em Chinês | MEDLINE | ID: mdl-17288837

RESUMO

OBJECTIVE: To assess the relationship between the insert-deletion polymorphism in the Human pepsinogen (PGC) gene and susceptibility to gastric cancer, together with its precursors, and to investigate the interaction between PGC polymorphism and H. pylori infection in the development of gastric cancer. METHODS: PGC gene polymorphism in 141 patients with gastric cancer and 564 matched non-cancer controls were detected by polymerase chain reaction (PCR), and the relation between the PGC polymorphism and gastric cancer was examined. Serum H. pylori-IgG was determined with ELISA method. The odds ratios and 95% confidence intervals (95% CI) were calculated using unconditional logistic regression model. The effect-modified model was used to evaluate the PGC-H. pylori interaction. RESULTS: No significant association between the frequency of homogenous allele 1 of PGC gene and the sex and age of the subjects was observed. The subjects with the homogenous allele 1 had an increased risk of developing atrophic gastritis (OR: 3.103, 95% CI: 1.440-6.686), and gastric cancer (OR: 2.962, 95% CI: 1.370-6.404), and Intestinal metaplasia (OR: 1.659, 95% CI: 0.998-2.757) comparing with those with the non-homogenous allele 1. For subjects carrying both homogenous allele 1 and H. pylori-IgG positive, there was an significant increased risk of developing atrophic gastritis and gastric cancer (likelihood test ratio test: P = 0.023, P = 0.005). CONCLUSION: PGC gene polymorphism may be associated with susceptibility to gastric cancer and atrophic gastritis. The PGC gene polymorphism and H. pylori infection seem to display an interaction in the development of gastric cancer.


Assuntos
Pepsinogênio C/genética , Polimorfismo Genético , Lesões Pré-Cancerosas/genética , Neoplasias Gástricas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Anticorpos Antibacterianos/sangue , Feminino , Mucosa Gástrica/microbiologia , Mucosa Gástrica/patologia , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Helicobacter pylori/imunologia , Humanos , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Lesões Pré-Cancerosas/microbiologia , Neoplasias Gástricas/microbiologia
18.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 21(4): 503-5, 2005 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-15989802

RESUMO

AIM: To explore the relationship between helicobacter pylori (Hp) infection and expression of interleukin-8 (IL-8) in the remnant stomach mucosa. METHODS: Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to detect Hp infection (by H.E. and Giemsa staining) and IL-8 expression (by real-time semi-quantitative RT-PCR). RESULTS: Hp infection was detected in 65.5% (38/58)of the remnant stomach mucosa. In 34 patients with Billroth I (BI) anastomosis, IL-8 in corpus was significantly higher in Hp-positive patients than that in Hp-negative patients (0.11+/-0.07 vs 0.02+/-0.01, P<0.05). In 24 patients with Billroth II (BII) anastomosis, IL-8 in stoma and corpus in Hp-positive patients was significantly higher than that in Hp-negative patients (P<0.05). CONCLUSION: Hp infection induces IL-8 expression in remnant stomach mucosa. In corpus, IL-8 mRNA expression is mainly related with Hp infection, while in stoma, IL-8 mRNA expressions may be related with bile reflux besides Hp infection.


Assuntos
Mucosa Gástrica/imunologia , Mucosa Gástrica/microbiologia , Coto Gástrico , Regulação da Expressão Gênica , Infecções por Helicobacter/genética , Helicobacter pylori , Interleucina-8/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Endoscópios Gastrointestinais , Feminino , Mucosa Gástrica/metabolismo , Mucosa Gástrica/cirurgia , Gastroenterostomia , Infecções por Helicobacter/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
19.
Zhonghua Wai Ke Za Zhi ; 43(3): 166-8, 2005 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-15842895

RESUMO

OBJECTIVE: To study the expressions and the significance of interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2) in the remnant stomach. METHODS: Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Two biopsy specimens were obtained from the stoma and the upper corpus gastric mucosa in the remnant stomach. mRNA was extracted from biopsy specimens to measure the IL-8 and COX-2 gene mRNA levels by real-time PCR method. RESULTS: IL-8 and COX-2 levels were higher in stoma than in corpus, IL-8 levels in BI anastomosis were significantly higher in stoma than in corpus (P< 0.05). In Hp-negative patients, IL-8 and COX-2 levels in stoma were significantly higher in BII anastomosis than in BI anastomosis (P < 0.05). In Hp-positive patients, IL-8 and COX-2 levels in stoma showed no significant differences between BII anastomosis and BI anastomosis. In corpus, IL-8 and COX-2 levels in Hp-positive patients were significantly higher than those in Hp-negative patients, (P < 0.05), including in BI anastomosis and in BII anastomosis. CONCLUSIONS: The risk of the secondary stomach carcinogenesis in stoma after distal gastrectomy is higher than that in corpus; The types of anastomosis may influence the risk for the secondary stomach carcinogenesis in the remnant stomach mucosa.


Assuntos
Mucosa Gástrica/metabolismo , Coto Gástrico/cirurgia , Gastroenterostomia/métodos , Interleucina-8/biossíntese , Prostaglandina-Endoperóxido Sintases/biossíntese , Neoplasias Gástricas/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Mucosa Gástrica/microbiologia , Gastroenterostomia/efeitos adversos , Infecções por Helicobacter , Helicobacter pylori , Humanos , Interleucina-8/genética , Masculino , Pessoa de Meia-Idade , Prostaglandina-Endoperóxido Sintases/genética , RNA Mensageiro/biossíntese
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 23(3): 576-8, 2003 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-12953546

RESUMO

A method for the determination of copper in hepatolenticular degeneration patient's serum and urine by flame atomic absorption spectrometry was developed, and the meaning of clinic diagnosis and therapy for copper lever of hepatolenticular degeneration patient in serum and urine was discussed. The detection limit and linear range for copper are 7.3 x 10(-3) micrograms.mL-1 and 0.008-5 micrograms.mL-1 respectively. The relative standard deviation is less than 1.4% and the recovery is 94%-104%. The experimental results show that serum copper and urine copper contents are really important reference index for diagnosing the disease, with guiding significance for timely monitoring and controlling serum copper and urine copper excretion, especially in therapy, although they do not have separate diagnosing significance for hepatolenticular degeneration disease and have to be combined with clinic symptoms and other biochemical indexes to make a comprehensive diagnosis. This method has been applied to clinic diagnosis and therapy with satisfactory results.


Assuntos
Cobre/sangue , Degeneração Hepatolenticular/sangue , Adolescente , Adulto , Biomarcadores , Criança , Cobre/urina , Feminino , Degeneração Hepatolenticular/urina , Humanos , Masculino , Valor Preditivo dos Testes , Espectrofotometria Atômica
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