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1.
J Am Soc Nephrol ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32029431

RESUMO

BACKGROUND: The genes and mechanisms involved in the association between diabetes or hypertension and CKD risk are unclear. Previous studies have implicated a role for γ-adducin (ADD3), a cytoskeletal protein encoded by Add3. METHODS: We investigated renal vascular function in vitro and in vivo and the susceptibility to CKD in rats with wild-type or mutated Add3 and in genetically modified rats with overexpression or knockout of ADD3. We also studied glomeruli and primary renal vascular smooth muscle cells isolated from these rats. RESULTS: This study identified a K572Q mutation in ADD3 in fawn-hooded hypertensive (FHH) rats-a mutation previously reported in Milan normotensive (MNS) rats that also develop kidney disease. Using molecular dynamic simulations, we found that this mutation destabilizes a critical ADD3-ACTIN binding site. A reduction of ADD3 expression in membrane fractions prepared from the kidney and renal vascular smooth muscle cells of FHH rats was associated with the disruption of the F-actin cytoskeleton. Compared with renal vascular smooth muscle cells from Add3 transgenic rats, those from FHH rats had elevated membrane expression of BKα and BK channel current. FHH and Add3 knockout rats exhibited impairments in the myogenic response of afferent arterioles and in renal blood flow autoregulation, which were rescued in Add3 transgenic rats. We confirmed these findings in a genetic complementation study that involved crossing FHH and MNS rats that share the ADD3 mutation. Add3 transgenic rats showed attenuation of proteinuria, glomerular injury, and kidney fibrosis with aging and mineralocorticoid-induced hypertension. CONCLUSIONS: This is the first report that a mutation in ADD3 that alters ACTIN binding causes renal vascular dysfunction and promotes the susceptibility to kidney disease.

2.
Nat Plants ; 6(2): 107-118, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32042158

RESUMO

Hornworts, liverworts and mosses are three early diverging clades of land plants, and together comprise the bryophytes. Here, we report the draft genome sequence of the hornwort Anthoceros angustus. Phylogenomic inferences confirm the monophyly of bryophytes, with hornworts sister to liverworts and mosses. The simple morphology of hornworts correlates with low genetic redundancy in plant body plan, while the basic transcriptional regulation toolkit for plant development has already been established in this early land plant lineage. Although the Anthoceros genome is small and characterized by minimal redundancy, expansions are observed in gene families related to RNA editing, UV protection and desiccation tolerance. The genome of A. angustus bears the signatures of horizontally transferred genes from bacteria and fungi, in particular of genes operating in stress-response and metabolic pathways. Our study provides insight into the unique features of hornworts and their molecular adaptations to live on land.

3.
J Org Chem ; 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32083864

RESUMO

An asymmetric Michael/hemiketalization and Fridel-Crafts reaction has been reported through one-pot reaction. A number of structurally novel tetrahyduofuran spirooxindoles are synthesized in the presence of 10 mol % dinuclear zinc catalyst with diastereomer ratio (dr) of 3:1 to 13:1 and enantiomeric excess (ee) of 75-99%. The reaction can be performed on a gram scale without impacting its efficiency. The absolute configuration of products is confirmed by X-ray single crystal structure analysis, and a possible mechanism is proposed.

4.
Artif Cells Nanomed Biotechnol ; 48(1): 648-655, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32070139

RESUMO

Recently, the functions of circular RNAs (circRNAs) on cancer initiation and development arouse wide concern. Herein, we tested the influences of circ-ZNF652 on renal carcinoma cell growth and metastasis. Firstly, clinical renal carcinoma tissues and corresponding normal tissues were collected. The circ-ZNF652 expressions were tested. Then, the influences of silencing circ-ZNF652 on renal carcinoma A498 and ACHN cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) process, as well as Ras/Raf/MEK/ERK and JAK1/STAT3 pathways, were probed. Finally, whether miR-205 engaged in the influences of silencing circ-ZNF652 on A498 and ACHN cell were investigated. circ-ZNF652 had high expression level in clinical renal carcinoma tissues. Silencing circ-ZNF652 repressed A498 and ACHN cell proliferation and EMT process, but promoted cell apoptosis. Moreover, silencing circ-ZNF652 suppressed Ras/Raf/MEK/ERK and JAK1/STAT3 pathways in A498 and ACHN cells. Besides, the miR-205 expressions in A498 and ACHN cells were raised by silencing circ-ZNF652. Knockdown of miR-205 weakened the influences of silencing circ-ZNF652 on A498 and ACHN cell proliferation, apoptosis and EMT process. Silencing circ-ZNF652 repressed proliferation and EMT process of renal carcinoma A498 and ACHN cells via suppressing Ras/Raf/MEK/ERK and JAK1/STAT3 pathways, as well as raising miR-205 expression.

5.
Viruses ; 12(2)2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32093360

RESUMO

With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus-host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially Deformed wing virus) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology.

6.
J Hazard Mater ; 386: 121999, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31901547

RESUMO

Design and fabrication of novel adsorbents to remove heavy metal ions in continuous-flow wastewater remained a great challenge. Inspired by the hierarchical architecture and biomineralization process of nacre, we firstly constructed hydroxyapatite/chitosan (HA/CH) layered composites. The brick-and-mortar characteristics of HA/CH layered composites improved their flexure strengths up to 3.08 MPa so that the hierarchical architectures could not be destroyed even under high-pressure drop. HA/CH layered composites had the hierarchical microstructures analogous to plate towers, facilitating the separation of adsorbents from water. The interlaminar macropores in the layered composites contributed to the transfer of continuous-flow wastewater. The Pb(II), Cd(II) and Hg(II) ions in wastewater showed similar adsorption trends, and their adsorption amounts arrived at 295.96, 192.37 and 127.38 mg g-1 after 6 days, respectively. Among the above heavy metal ions, the HA/CH layered composites possessed the best Pb(II) adsorption ability due to forming lead hydroxyapatite rods and CH-Pb complexes. The Pb(II) adsorption performances of HA/CH layered composites matched well with Elovich equation, pseudo-first-order and pseudo-second-order kinetics models, revealing the heterogeneous chemisorption mechanism at adsorbent/wastewater interfaces. Therefore, the nacre-like HA/CH layered composites with appropriate mechanical property and excellent adsorption capacity are a novel platform for heavy metal removal in continuous-flow wastewater.

7.
Nat Prod Res ; : 1-8, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31960729

RESUMO

The anti-tumor effects of two compounds purified from Sapindus mukorossi Gaertn. (S. mukorossi.) on breast cancer in vitro were observed. Their chemical structures were identified as sesquiterpene glycosides, namely, Mukurozioside IIa and Mukurozioside IIb. The results of XTT assay indicated that their inhibition rates against three cancer cell lines (MCF-7, MDA-MB-231 and MDA-MB-435s) reached approximately 80% at a concentration of 200 µg/mL, which were higher than that of cyclophosphamide (below 40% at 200 µg/mL), and their 50% inhibiting concentrations were ranged from 120.73 to 154.01 µg/mL, indicating their inhibition were weaker than their parent fraction. Furthermore, the mechanism on breast cancer was predicted, and 22 targets including PTPN1, IL2 and VEGFA were relatively important. These results illustrated the anti-breast cancer activity of S. mukorossi was related to the two compounds with the structure of sesquiterpene glycosides, but they did not represent the full activity of their parent fraction.

8.
Nat Prod Res ; : 1-6, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31994913

RESUMO

This study evaluated the antibacterial activities of different extracts of Sapindus mukorossi Gaertn. (S. mukorossi) on Cutibacterium acnes (C. acnes). The extract solvent and procedure were screened, based on the yield of saponins and minimum inhibitory concentration (MIC). The results showed that the optimized product, fermentation and ethyl acetate extract by adding isoamyl alcohol from water extract of S. mukorossi (SWFEAI), had the highest yield of saponins (7.83 ± 0.26%) and the best antibacterial activity (MIC = 0.125 mg/mL) on C. acnes. The destroyed bacterial cell membrane and wall were observed by transmission electron microscopy, which then resulted in cell lysis and death. Furthermore, 20 compounds of SWFEAI were detected, among which oleanane-type triterpenoid saponins with molecular weights of 734, 750, 882, 924 and 966 were speculated to contribute to the antibacterial activities of SWFEAI. The results showed that SWFEAI could be a natural anti-acne agent.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31965787

RESUMO

The electrochemical nitrogen reduction reaction (NRR) is a very efficient method for sustainable NH3 production, but it requires effective catalysts to expedite the NRR kinetics and inhibit the concomitant hydrogen evolution reaction (HER). Two-dimensional (2D)/2D interface engineering is an effective method to design powerful catalysts due to intimate face-to-face contact of two 2D materials that facilitates the strong interfacial electronic interactions. Herein, we explored a 2D/2D MoS2/C3N4 heterostructure as an active and stable NRR catalyst. MoS2/C3N4 exhibited a conspicuously improved NRR performance with an NH3 yield of 18.5 µg h-1 mg-1 and a high Faradaic efficiency (FE) of 17.8% at -0.3 V, far better than those of the individual MoS2 or C3N4 component. Density functional theory calculations revealed that the interfacial charge transport from C3N4 to MoS2 could enhance the NRR activity of MoS2/C3N4 by promoting the stabilization of the key intermediate *N2H on Mo edge sites of MoS2 and concurrently decreasing the reaction energy barrier. Meanwhile, MoS2/C3N4 rendered a more favorable *H adsorption free energy on S edge sites than on Mo edge sites of MoS2, thereby protecting the NRR-active Mo edge sites from the competing HER and leading to a high FE.

10.
Physiol Rep ; 8(2): e14345, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31960618

RESUMO

We recently reported that KO of Dual-specificity protein phosphatase 5 (Dusp5) enhances myogenic reactivity and blood flow autoregulation in the cerebral and renal circulations in association with increased levels of pPKC and pERK1/2 in the cerebral and renal arteries and arterioles. In the kidney, hypertension-related renal damage was significantly attenuated in Dusp5 KO rats. Elevations in pPKC and pERK1/2 promote calcium influx in VSMC and facilitate vasoconstriction. However, whether DUSP5 plays a role in altering the passive mechanical properties of cerebral and renal arterioles has never been investigated. In this study, we found that KO of Dusp5 did not alter body weights, kidney and brain weights, plasma glucose, and HbA1C levels. The expression of pERK is higher in the nucleus of primary VSMC isolated from Dusp5 KO rats. Dusp5 KO rats exhibited eutrophic vascular hypotrophy with smaller intracerebral parenchymal arterioles and renal interlobular arterioles without changing the wall-to-lumen ratios. These arterioles from Dusp5 KO rats displayed higher myogenic tones, better distensibility, greater compliance, and less stiffness compared with arterioles from WT control rats. VSMC of Dusp5 KO rats exhibited a stronger contractile capability. These results demonstrate, for the first time, that DUSP5 contributes to the regulation of the passive mechanical properties of cerebral and renal arterioles and provide new insights into the role of DUSP5 in vascular function, cancer, stroke, and other cardiovascular diseases.

11.
J Minim Invasive Gynecol ; 27(1): 94-99, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31212071

RESUMO

STUDY OBJECTIVE: Cesarean scar pregnancy (CSP) is a rare type of ectopic pregnancy, and a significant concern in the management of this condition is the control and prevention of bleeding. We aimed to determine the efficacy and value of an indwelling, intrauterine Foley balloon catheter in controlling and preventing intraoperative and postoperative bleeding in patients with CSP. DESIGN: Retrospective case series. SETTING: University-affiliated hospital. PATIENTS: Between January 1, 2015 and May 31, 2017, 70 patients presented with CSP. INTERVENTIONS: All patients underwent uterine curettage under hysteroscopic guidance and ultrasound monitoring. Patients were then assigned to 2 groups: the study group, with an indwelling Foley balloon catheter placed in the uterine cavity during surgery and retained for 24 to 48 hours, and the control group, without catheter placement. Data were collected to compare the 2 groups in terms of intraoperative and postoperative complications, surgical time, and status of menstruation resumption. MEASUREMENTS AND MAIN RESULTS: The average daily volume of postoperative blood loss during the first 3 postoperative days in the study group was 23.1 mL compared with 31.5 mL observed in the control group (p = .041). Moreover, the study group had significantly shorter average duration of bleeding (p = .027) and fewer menstruation abnormalities than the control group. Uterine ultrasonography performed after resumption of menstruation showed that none of the enrolled patients had any intrauterine abnormalities. CONCLUSIONS: The use of an indwelling, intrauterine Foley balloon catheter has positive results in the management of CSP.

12.
Photosynth Res ; 2019 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-31813097

RESUMO

In this work, the main activities of the plant photosynthesis process are discussed to yield a minimized mathematical model structure with photosystem II (PSII) chlorophyll a fluorescence (ChlF) as a measurable output. After experimental validation of the model structure, we demonstrate that the states of the photosynthetic process may be observed by using this model and the extended Kalman filter method. We then show a feedback control framework that can be used to alter a given photosynthetic activity. The control framework is demonstrated with an example in which PSII ChlF is used as the feedback signal and light intensity is used as a controllable process input to regulate plastoquinone reduction. Although there are caveats, and further research is needed, the results lay the groundwork for further research on novel methods for optimization and regulation of photosynthetic activities, with a goal for sustainability.

13.
Ann Transl Med ; 7(20): 532, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31807514

RESUMO

Background: Pancreatic stellate cells (PSCs) is a highly heterogeneic stroma cell population in pancreatic cancer tissue. Interaction between PSCs and pancreatic cancer cells has not been well elucidated. This research was aimed to study the relationship between fibroblast activation protein α (FAPα)-positive (FAPα+) PSCs and the pathological features and prognosis of pancreatic cancer. The effects and mechanisms of FAPα + PSCs in pancreatic cancer were also explored. Methods: Tissue microarray analysis was used to detect FAPα expression in tumor and adjacent tissues. The relationship between FAPα expression and pancreatic pathological features and prognosis were analyzed. The effects of FAPα+ PSCs on the proliferation, migration and invasion of pancreatic cancer were detected in vitro and in vivo. A cytokine chip was used to detect the differential expression of cytokines in FAPα-positive (FAPα+) and FAPα-negative (FAPα-) PSCs. Phosphorylated tyrosine kinase receptors were detected by a human phosphotyrosine kinase receptor protein chip. The interaction between differential cytokine and tyrosine kinase receptors was detected by immunoprecipitation. Results: Compared with the adjacent tissues, pancreatic cancer stromal tissues showed high FAPα expression. FAPα was mainly expressed in the PSCs. FAPα+ PSCs were associated with lymph node metastasis. Higher numbers of FAPα+ PSCs predicted shorter survival. Pancreatic cancer cells released TGFß1 and induced PSCs to express FAPα. FAPα+ PSCs released the chemokine CXCL1 and promoted the phosphorylation of the tyrosine kinase receptors EphB1 and EphB3 in pancreatic cancer cells. CXCL1, EphrinB1, and EphrinB3 worked together to promote the migration and invasion of pancreatic cancer cells by Akt phosphorylation. Talabostat (PT100), an FAPα inhibitor, inhibited the roles of FAPα+ PSCs. Conclusions: FAPα+ PSCs can promote the migration, invasion, and metastasis of pancreatic cancer by the Akt signaling pathway. This interaction of FAPα+ PSCs with pancreatic cancer cells may become a new strategy for the comprehensive treatment of pancreatic cancer.

14.
Biomed Res Int ; 2019: 2408348, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31828095

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies. Recent studies reveal that tumor microenvironment (TME) components significantly affect HCC growth and progression, particularly the infiltrating stromal and immune cells. Thus, mining of TME-related biomarkers is crucial to improve the survival of patients with HCC. Public access of The Cancer Genome Atlas (TCGA) database allows convenient performance of gene expression-based analysis of big data, which contributes to the exploration of potential association between genes and prognosis of a variety of malignancies, including HCC. The "Estimation of STromal and Immune cells in MAlignant Tumors using Expression data" algorithm renders the quantification of the stromal and immune components in TME possible by calculating the stromal and immune scores. Differentially expressed genes (DEGs) were screened by dividing the HCC cohort of TCGA database into high- and low-score groups according to stromal and immune scores. Further analyses of functional enrichment and protein-protein interaction networks show that the DEGs are mainly involved in immune response, cell adhesion, and extracellular matrix. Finally, seven DEGs have significant association with HCC poor outcomes. These genes contain FABP3, GALNT5, GPR84, ITGB6, MYEOV, PLEKHS1, and STRA6 and may be candidate biomarkers for HCC prognosis.

15.
Immunopharmacol Immunotoxicol ; 41(6): 599-606, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31691624

RESUMO

Purpose: Immunotherapy has demonstrated durable clinical responses in various cancers by disinhibiting the immune system, largely attributed to the success of immune-checkpoint blockade. However, there are still subsets of patients across multiple cancers not showing robust responses to these agents and one significant barrier to their efficacy may be the recruitment of myeloid-derived suppressor cells (MDSCs) into the tumor microenvironment. In this study, we demonstrated that functional inhibition of MDSCs with (3 R)-5,6,7-trihydroxy-3-isopropyl-3-methylisochroman-1-one (TIMO), a potent PI3Kδ/γ inhibitor, enhanced the therapeutic efficacy of anti-PD1 antibody in the tumor model.Materials and methods: A syngeneic ovarian tumor model was established. MDSCs from the peripheral blood and tumor parenchyma were analyzed by flow cytometry. Proliferation and killing effects of T-lymphocytes were measured. IFNγ production was measured by ELISA assay. qPCR and western blot were used to detect the gene and protein expression. Furthermore, the therapeutic effects of TIMO combined with anti-PD1 antibody were assessed by the tumor model.Results: Our data demonstrated that inhibition of granulocytic myeloid-derived suppressor cells (G-MDSCs) function with TIMO could overcome MDSCs-mediated immunosuppression and promote antigen-specific T-lymphocyte responses, resulting in the restoration of cytotoxic T cell-mediated tumor control. We further demonstrated that TIMO and anti-PD1 combination therapy promoted tumor growth control in a syngeneic ovarian tumor model.Conclusions: Our results provided proof of concept for a new combination strategy involving the use of a selective inhibitor of PI3Kδ/γ to inhibit the function of MDSCs to enhance tumor responses to immune checkpoint blocking antibodies.

16.
IET Syst Biol ; 13(6): 327-332, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31778129

RESUMO

The emission of chlorophyll fluorescence (ChlF) from photosystem II (PSII) of plant leaves the couple with photoelectron transduction cascades in photosynthetic reactions and can be used to probe photosynthetic efficiency and plant physiology. Because of population increase, food shortages, and global warming, it is becoming more and more urgent to enhance plant photosynthesis efficiency by controlling plant growth rate. An effective model structure is essential for plant control strategy development. However, there is a lack of reporting on modelling and simulation of PSII activities under the interaction of both illumination light intensities and temperatures, which are the two important controllable factors affecting, plant growth, especially for a greenhouse. In this work, the authors extended their work on modelling photosynthetic activities as affected by light and temperature to cover both the interaction effects of illumination light intensities and temperature on ChlF emission. Experiments on ChlF were performed under different light intensities and temperatures and used to validate the developed model structure. The average relative error between experimental data and model fitting is <0.3%, which shows the effectiveness of the developed model structure. Simulations were performed to show the interaction effect of light and temperature effects on photosynthetic activities.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31675289

RESUMO

A Gram-stain-negative, rod-shaped, non-motile, poly-ß-hydroxybutyrate-accumulating and aerobic bacterial strain, designated CHR27T, was isolated and characterized by using the polyphasic taxonomy approach. The results of phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CHR27T is affiliated with species in the genus Sphingobium. 16S rRNA gene sequence similarity results indicated that strain CHR27T was closely related to species of the genus Sphingobium (94.3-97.0 %), and had the highest sequence similarity to Sphingobium qiguonii X23T (97.0 %). Strain CHR27T showed 19.4-22.1 % digital DNA-DNA hybridization values and 73.2-74.8 % average nucleotide identity values with the strains of other Sphingobium species. Optimal growth occurred at 25 °C, pH 7.5 and in the absence of NaCl. The major fatty acids of strain CHR27T were C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The predominant hydroxy fatty acid was C14 : 0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, two unidentified sphingoglycolipids and an unidentified aminophospholipid. Strain CHR27T contained spermidine as the major polyamine and putrescine as a minor component. The only isoprenoid quinone was ubiquinone-10. The genomic DNA G+C content of strain CHR27Twas 61.8 mol%. On the basis of the phylogenetic inference and phenotypic data, strain CHR27T was considered a representative of a novel species within the genus Sphingobium. The name Sphingobium fluviale sp. nov. is proposed, with strain CHR27T (=BCRC 81121T=LMG 30596T=KCTC 62510T) as the type strain.

18.
Ann Bot ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31641747

RESUMO

BACKGROUND: With limited agricultural land and increasing human population, it is essential to enhance overall photosynthesis, and thus, productivity. Oxygenic photosynthesis begins with light absorption, followed by excitation energy transfer to the reaction centers, primary photochemistry, electron and proton transport, ATP synthesis, and then CO2 fixation (Calvin-Benson cycle, as well as Hatch-Slack cycle). We mention here some of the discoveries related to this process, such as the existence of two light reactions and two photosystems connected by an electron transport 'chain' (the Z-scheme), chemiosmotic hypothesis for ATP synthesis, water oxidation clock for oxygen evolution, steps for carbon fixation, and finally the diverse mechanisms of regulatory processes, such as "state transitions" and "non-photochemical quenching" of the excited state of chlorophyll a. SCOPE: In this review, we emphasize that mathematical modeling is a highly valuable tool in understanding and making predictions on photosynthesis. Different mathematical models have been used to examine current theories on diverse photosynthetic processes; these have been validated through simulation(s) of available experimental data, such as chlorophyll a fluorescence induction, measured with fluorometers using continuous (or modulated) exciting light, and absorbance changes at 820 nm (ΔA820 ) related to redox changes in P700, the reaction center of Photosystem I. CONCLUSIONS: We show in this review the important role of modeling in deciphering and untangling complex photosynthesis processes taking place simultaneously, as well as in predicting possible ways to obtain higher biomass and productivity in plants, algae and cyanobacteria.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31596696

RESUMO

Strain TLA-22T, isolated from a cold spring in Taiwan, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, poly-ß-hydroxybutyrate-accumulating, motile by means of a single polar flagellum, rod-shaped and formed bright yellow colonies. Optimal growth occurred at 20-25 °C, pH 6-6.5, and in the presence of 0.5 % NaCl. The major fatty acids of TLA-22T were C18 : 1 ω7 c and C17 : 1ω6c. The predominant hydroxy fatty acids were C15 : 0 2-OH and C14 : 0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sphingoglycolipid, an unidentified aminophospholipid, an unidentified phospholipid and three unidentified lipids. TLA-22T contained spermidine as the major polyamine and putrescine as the minor component. The only isoprenoid quinone was Q-10. The genomic DNA G+C content of TLA-22T was 63.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that TLA-22T was a mem,ber of a phylogenetic lineage including members of the genus Sphingobium. TLA-22T was most closely related to Sphingobium aromaticiconvertens RW16T, with a 97.4 % 16S rRNA gene sequence similarity. TLA-22T showed 74.8-75.7 % average nucleotide identity and 20.1-22.0 % digital DNA-DNA hybridization identity with the strains of other species of the genus Sphingobium. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TLA-22T should be classified as representing a novel species of the genus Sphingobium, for which the name Sphingobium algorifonticola sp. nov. is proposed. The type strain is TLA-22T (=BCRC 81097T =LMG 30309T=KCTC 62189T).

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