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1.
Medicine (Baltimore) ; 97(1): e9334, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29505514

RESUMO

RATIONALE: Adult spinal epidermoid cyst (SEC) is a rare tumor. Lumbar laminectomy and tumor removal was a routine surgical procedure for adult spinal epidermoid cyst according to the literature, but postoperative lumbar instability and intractable low back pain may occur. In this study, we presented a brief report of an adult lumbar epidermoid cyst and introduced another surgical approach. PATIENT CONCERNS: This 28-year-old woman has been complaining of the severe right buttock pain and right thigh radiating pain for half a year. She had been diagnosed as sacroiliitis, spinal arthritis, and lumbar disc herniation at 3 different hospitals before coming to our hospital. And she received a variety of conservative treatments, including non-steroidal anti-inflammatory drugs, aspirin, acetaminophen, glucocorticoids, acupuncture, physical therapy, and so on. However, her pain did not diminish at all. Finally, we find a space-occupying lesion in her lumbar magnetic resonance images (MRI). The lesion was slightly low, equal, and uneven equal-low signals on T1WI. T2WI showed slightly higher, equal, and uneven equal-high signals. And a thin-rim enhancement was observed on Gd-DTPA-enhanced MRI. DIAGNOSES: Adult spinal epidermoid cyst. INTERVENTIONS: The patient underwent a surgery of lumbar laminectomy, tumor excision, and spinous process-vertebral plate in situ replantation. OUTCOMES: Postoperative pathology prompted that the tumor was cystoid. The patient's symptoms were completely removed 1 week after surgery. Three-month postoperative MRI confirmed that the spinal epidermoid cyst had been completely removed and three-dimensional CT prompted lumbar lamina in situ. Bony fusion occurred at 6 months after the surgery. LESSONS: Lumbar laminotomy and replantation provides an ideal option to treat adult spinal epidermoid cyst because it can completely remove the cyst and simultaneously reduce the risk of iatrogenic lumbar instability.


Assuntos
Cisto Epidérmico/cirurgia , Laminectomia/métodos , Vértebras Lombares/cirurgia , Reimplante , Doenças da Coluna Vertebral/cirurgia , Adulto , Cisto Epidérmico/diagnóstico por imagem , Feminino , Humanos , Vértebras Lombares/diagnóstico por imagem , Doenças da Coluna Vertebral/diagnóstico por imagem
2.
Biosci Rep ; 37(3)2017 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-28536312

RESUMO

The ginsenoside Rg1 is the most abundant compound in ginseng. Recent studies showed that Rg1 had neuroprotective effects on neuronal cells. The present study was to prepare Rg1-loaded alginate-chitosan microspheres and research the effects of microspheres on human bone marrow (BM) stromal cells (hBMSC). The alginate-chitosan microspheres were prepared by mechanical emulsification technique in combination with ion (Ca2+) and chitosan solidification. Subsequently, the microspheres were employed to load Rg1 ginseng extracts. The microspheres had a smooth surface and were spherical in shape. The average diameter of the microspheres was 3.95 µm. The loading efficiency was approximately 2.12%. The purity of isolated hBMSC was over 98.8%. Rg1-loaded microspheres could promote hBMSC proliferation and differentiation. Meanwhile, Rg1-loaded microspheres could also suppress hBMSC apoptosis induced by hypoxia-reoxygenation. In conclusion, these loaded microspheres may be used in the research of neuroprotective effects of Rg1.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Ginsenosídeos/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Alginatos/química , Células Cultivadas , Quitosana/química , Portadores de Fármacos/química , Medicamentos de Ervas Chinesas/administração & dosagem , Ginsenosídeos/administração & dosagem , Ácido Glucurônico/química , Ácidos Hexurônicos/química , Humanos , Células-Tronco Mesenquimais/citologia , Fármacos Neuroprotetores/administração & dosagem , Tamanho da Partícula
3.
Molecules ; 22(5)2017 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-28534831

RESUMO

Quantitative determination of multiple effective components in a given plant usually requires a very large amount of authentic natural products. In this study, we proposed a rapid and non-destructive method for the simultaneous determination of echinacoside, verbascoside, mannitol, sucrose, glucose and fructose in Cistanche tubulosa by near infrared spectroscopy (NIRS). Near infrared diffuse reflectance spectroscopy (DRS) and high performance liquid chromatography (HPLC) were conducted on 116 batches of C. tubulosa samples. The DRS data were processed using standard normal variety (SNV) and multiplicative scatter correction (MSC) methods. Partial least squares regression (PLSR) was utilized to build calibration models for components-of-interest in C. tubulosa. All models were then assessed by calculating the root mean square error of calibration (RMSEC), correlation coefficient of calibration (r). The r values of all six calibration models were determined to be greater than 0.94, suggesting each model is reliable. Therefore, the quantitative NIR models reported in this study can be qualified to accurately quantify the contents of six medicinal components in C. tubulosa.


Assuntos
Cistanche/química , Frutose/isolamento & purificação , Glucose/isolamento & purificação , Glucosídeos/isolamento & purificação , Glicosídeos/isolamento & purificação , Manitol/isolamento & purificação , Fenóis/isolamento & purificação , Sacarose/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Extração Líquido-Líquido/métodos , Metanol , Extratos Vegetais/química , Solventes , Espectroscopia de Luz Próxima ao Infravermelho , Fatores de Tempo
4.
Springerplus ; 5(1): 2004, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27933260

RESUMO

INTRODUCTION: Beijing subway line 14 includes four stations and approximately 2.8 km of tunnels between the Dongfengbeiqiao and Jingshunlu areas of the city. Due to the surface and underground space limitations of this section, a double-track running tunnel instead of two single-track running tunnels was adopted to connect the two stations. The double-track tunnels were excavated by a 10.22 m diameter earth pressure balance (EPB) shield. It was the first time that an EPB shield more than 10 m in diameter was used in Beijing subway construction. CASE DESCRIPTION: The shield, which passes underneath densely built-up areas of the city and is equipped with a spoke-type cutterhead, with balance between the ground pressure and the earth chamber pressure at the tunnel face, is of great importance. Referring to experiences gained in the EPB shield tunneling, attention was paid to the function of soil conditioning and simultaneous backfilling grouting of the shield, and some special designs were considered in manufacturing the machine. DISCUSSION AND EVALUATION: In addition to the agitating rods welded to the cutterhead, two independently driven agitators were added to fully mix everything in the earth chamber. Independent pipelines were arranged for injecting different conditioning agents. Indoor tests in combination with field tests were conducted to find suitable additives and injection ratios of the additives, and determine the mix ratio of the two-component grout for simultaneous backfilling grouting. A scheme was employed for simultaneously injecting the bentonite slurry at 8% concentration and the foam liquid at 5% concentration to condition the excavated soil. The cement-sodium silicate grout was adopted to fill the tail void and the injection volume per ring was 14.1-15.3 m3. CONCLUSIONS: The performance of the shield and evaluation of the corresponding tunneling technologies are introduced in terms of the shield tunneling induced ground surface settlements. The success of the project is of great significance to Beijing subway construction and underground space utilization. The findings serve as a useful reference for similar projects.

5.
PeerJ ; 4: e1991, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27231648

RESUMO

Physcion and chrysophanol induce defense responses against powdery mildew in cucumbers. The combination of these two compounds has synergistic interaction against the disease. We performed RNA-seq on cucumber leaf samples treated with physcion and chrysophanol alone and with their combination. We generated 17.6 Gb of high-quality sequencing data (∼2 Gb per sample) and catalogued the expressions profiles of 12,293 annotated cucumber genes in each sample. We identified numerous differentially expressed genes that exhibited distinct expression patterns among the three treatments. The gene expression patterns of the Chr and Phy treatments were more similar to each other than to the Phy × Chr treatment. The Phy × Chr treatment induced the highest number of differentially expressed genes. This dramatic transcriptional change after Phy × Chr treatment leaves reflects that physcion combined with chrysophanol treatment was most closely associated with induction of disease resistance. The analysis showed that the combination treatment caused expression changes of numerous defense-related genes. These genes have known or potential roles in structural, chemical and signaling defense responses and were enriched in functional gene categories potentially responsible for cucumber resistance. These results clearly demonstrated that disease resistance in cucumber leaves was significantly influenced by the combined physcion and chrysophanol treatment. Thus, physcion and chrysophanol are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the defense response.

6.
Zhong Yao Cai ; 38(2): 215-20, 2015 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-26415392

RESUMO

OBJECTIVE: To study the impacts of lead (Pb) stress on the leaf photosynthetic pigment and the growth of Rabdosia rubescens,in order to provide a basis for planting area selection and growth regulation. METHODS: Taking chlorophyll a, chlorophyll b, carotenoids, growth rate, biomass and Pb content as the indexes, the Ramets hydroponic experiments at Pb concentration levels (135,270 and 540 mg/L) in the time(20, 35 and 50 d) were carried out. Photosynthetic pigment content was determined by spectrophotometer, and Pb mass fraction was detected with plasma emission spectrometer. RESULTS: There was uncertain effect on chlorophyll and carotenoid synthesis in different Pb concentrations in-early period (20 and 35 d). At the time of 50 d, the chlorophyll content was higher in the low-mid Pb concentrations, significantly lower in the high Pb concentration compared with the control group, and there were no significant differences on carotenoid contents in different Pb concentrations. CONCLUSION: Low-mid Pb concentrations can promote chlorophyll synthesis, and the bioaccumulation of high Pb concentration can inhibit the chlorophyll synthesis, and then restrict the growth of Rabdosia rubescens.


Assuntos
Carotenoides/química , Clorofila/química , Isodon/efeitos dos fármacos , Chumbo/farmacologia , Fotossíntese , Biomassa , Clorofila A , Isodon/crescimento & desenvolvimento , Folhas de Planta/química
7.
Front Plant Sci ; 6: 396, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26113849

RESUMO

Rehmannia glutinosa, an herb of the Scrophulariaceae family, is widely cultivated in the Northern part of China. The tuberous root has well-known medicinal properties; however, yield and quality are threatened by abiotic and biotic stresses. Understanding the molecular process of tuberous root development may help identify novel targets for its control. In the present study, we used Illumina sequencing and de novo assembly strategies to obtain a reference transcriptome that is relevant to tuberous root development. We then conducted RNA-seq quantification analysis to determine gene expression profiles of the adventitious root (AR), thickening adventitious root (TAR), and the developing tuberous root (DTR). Expression profiling identified a total of 6794 differentially expressed unigenes during root development. Bioinformatics analysis and gene expression profiling revealed changes in phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant hormone biosynthesis during root development. Moreover, we identified and allocated putative functions to the genes involved in tuberous root development, including genes related to major carbohydrate metabolism, hormone metabolism, and transcription regulation. The present study provides the initial description of gene expression profiles of AR, TAR, and DTR, which facilitates identification of genes of interest. Moreover, our work provides insights into the molecular mechanisms underlying tuberous root development and may assist in the design and development of improved breeding schemes for different R. glutinosa varieties through genetic manipulation.

8.
ChemMedChem ; 9(4): 714-8, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24403015

RESUMO

A series of ruthenium(II) polypyridyl complexes were synthesized and evaluated for their in vitro anticancer activities. The results showed that ruthenium polypyridyl complexes, especially [Ru(bpy)2 (p-tFPIP)](2+) (2 a; bpy=bipyridine, tFPIP=2-(2-trifluoromethane phenyl)imidazole[4,5-f][1,10]phenanthroline), exhibited novel anticancer activity against human cancer cell lines, but with less toxicity to a human normal cell line. The results of flow cytometry and caspase activities analysis indicated that the 2 a-induced growth inhibition against MG-63 osteosarcoma cells was mainly caused by mitochondria-mediated apoptosis. DNA fragmentation and nuclear condensation as detected by TUNEL-DAPI co-staining further confirmed 2 a-induced apoptotic cell death. Further, fluorescence imaging revealed that ruthenium(II) polypyridyl complexes could target mitochondria to induce mitochondrial fragmentation, accompanied by depletion of mitochondrial membrane potential. Taken together, these findings suggest a potential application of theses ruthenium(II) complexes in the treatment of cancers.


Assuntos
Antineoplásicos/farmacologia , Complexos de Coordenação/farmacologia , Mitocôndrias/efeitos dos fármacos , Polímeros/química , Piridinas/química , Rutênio/química , Antineoplásicos/síntese química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Complexos de Coordenação/síntese química , Complexos de Coordenação/química , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Células MCF-7 , Mitocôndrias/metabolismo , Estrutura Molecular , Relação Estrutura-Atividade , Células Tumorais Cultivadas
9.
Zhongguo Zhong Yao Za Zhi ; 39(21): 4216-21, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25775796

RESUMO

The seeds of Rabdosia rubescens were as the materials to research the impacts of different lead (Pb2+) concentrations(0, 135, 270, 540, 1 080 mg x L(-1)) on seed germination and seedling growth. The results show that: Low concentration of lead had no obvious effect on early germination of the seed, the germination vigor and germination speed were lightly higher but not significantly differed at the level of Pb concentration 135 mg x L(-1) with control group; Mid-high concentration of Pb solution (270-1 080 mg x L(-1)) significantly inhibited the seed germination and seedling growth, which reduced the seed germination rate, germination vigor, germination index, embryo root length and shoot length, growth index with increasing of Pb concentrations. There was a inhibitory effect on embryo shoot length and root length at mid-high lead concentrations stress, and stronger inhibitory effect on root , which was more sensitive than shoot to Pb stress(P < 0.05). Pb bioaccumulation coefficient (BC) was 0.76-2.59, increased with concentration of Pb; Pb enrichment in seedling mainly caused the growth inhibition. The fitting model predictive analyses show, the critical concentration of Pb, which causes the germination rate and biomass fresh weight reducing 10%, is 195.18, 101.65 mg x L(-1).


Assuntos
Germinação/efeitos dos fármacos , Isodon/efeitos dos fármacos , Chumbo/toxicidade , Plântula/crescimento & desenvolvimento , Estresse Fisiológico , Isodon/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento
10.
Phytother Res ; 28(1): 110-9, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23512684

RESUMO

Echinacoside (ECH) is a major bioactive phenyethanoids in medicinal herba Cistanche and has been reported to have antiinflammatory activity and beneficial effect on wound healing in many experimental studies. This study was to test the efficacy of ECH-enriched extract of Cistanche tubulosa in the treatment of dextran sulphate sodium (DSS)-induced colitis, a preclinical model of ulcerative colitis. Oral administration of ECH extract significantly suppresses the development of acute colitis, indicated by lowering disease activity index (p < 0.0001, n = 8) and preventing colonic damage (p = 0.0336). Histological examinations showed that ECH extract treatment protected intestinal epithelium from inflammatory injury (p = 0.0249) but had less effect on inflammatory cellular infiltration (p = 0.1753). The beneficial effect of ECH extract treatment was associated with upregulation of transforming growth factor (TGF)-ß1 as well as with an increase in the number of Ki67(+) proliferating cells in diseased colons (p < 0.0001). In cultured MODE-K cells, the addition of ECH extract enhanced in vitro wound healing that depended on TGF-ß1 expression. These data suggest that ECH extract possesses a greater efficacy in preventing DSS-induced colitis in mice, implying the potential of ECH or its derivatives for clinically treating inflammatory bowel disease.


Assuntos
Anti-Inflamatórios/farmacologia , Cistanche/química , Colite/tratamento farmacológico , Glicosídeos/farmacologia , Extratos Vegetais/farmacologia , Animais , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Colite/induzido quimicamente , Colite/patologia , Colite Ulcerativa , Colo/efeitos dos fármacos , Colo/patologia , Sulfato de Dextrana/efeitos adversos , Modelos Animais de Doenças , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Fator de Crescimento Transformador beta1/uso terapêutico , Cicatrização
11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(3): 804-7, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23705458

RESUMO

ICP-AES technology was used to determine the major mineral elements content and analyze the dynamic and cumulative amount of the main absorption during the growth and development stage of the Kinlowii organs plant. The result showed as follows: (1)The mineral elements were changing in the different stage. The highest mineral element accumulation of K, P, Ca, Mg, Fe, Mn, Zn, Cu and Mo occured in October, their values are 3,695.90, 445.88, 9,649.32, 2,652.10 mg per plant, 324,398.29, 40,188.65, 22,383.13, 36,054.58 and 61.95 microg per plant separately. But the highest value of B occured in September and the value was 8 690.97 microg per plant. (2) the distribution of mineral element in the kirilowii plant was not even, the contents of K and P were highest in kirilowii peel, they can reach 27.65 and 2.63 mg.g-1. The contents of Ca and Mg were highest in kirilowii leaves, they can reach 33.28 and 5.73 mg .g-1. The contents of Fe, Mn, B were highest in kirilowii roots, they can reach: 4,069.74, 127.73, 24.75 microg.g-1. The contents of Zn and Mo were highest in kirilowii seeds, they can reach 68. 61 and 1.07 microg.g-1. (3) the cumulation dynamic of mineral elements in kirilowii plant during the whole stages was S-type, and the plant showed rapid growth from the mid-July to mid-Spetember. The information of this study will supply a scientific data for the quality assessment and rational fertilization of kirilowii plant and harvestment.


Assuntos
Espectrofotometria Atômica/métodos , Oligoelementos/análise , Trichosanthes/química , Trichosanthes/crescimento & desenvolvimento , Oligoelementos/química
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(3): 813-6, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23705460

RESUMO

Determination of Fe, Zn, Cu, K, P, Ca, Mg, Na, B and Al in cistanche and its extractives was carried out by the inductively coupled plasma atomic emission spectrometry (ICP-AES). The results showed that (1) the contents of Mn, K, Ca, Mg and Na in cistanche deserticola Y. C. Ma were 17.70, 16,278. 03, 1,947.25, 1,662.76, and 106.79 respectively (microg.g-1), much higher than in Cistanche tubulosa (Schrenk) Wight; (2) the contents of Fe, Cu and P in 50% methanol extract of Cistanche deserticola were 1.5, 2.4, and 1.1 times that of aqueous extract respectively, the contents of Mn, Zn, K, P, Mg and Na were 3.8, 1.2, 1.6, 2.3, 2.2, and 1.1 times that of methanol extract respectively; (3) the contents of Cu and P in 50% methanol extract of Cistanche tubulosa were 1.6 and 1.2 times that of aqueous extract, the contents of Mn, Zn, K, P, Mg, Na and Al were 2.0, 1.6, 1.6, 1.4, 1.8, 1.2, and 1.2 times that of methanol extract respectively; (4) the leaching rate of 11 mineral elements in Cistanche deserticola and Cistanche tubulosa were 60.79%-83. 10% and 44. 58%-83.84% in 50% methanol respectively, and the leaching rate of Mn, Zn, K, P, Ca, Na and Al were much higher than in water and 100% methanol extracted. The research provided the basic data of mineral element in comparative study on Cistanche deserticola and Cistanche tubulosa, and provide scientific basis for efficient utilization of mineral element in cistanche.


Assuntos
Cistanche/química , Extratos Vegetais/química , Espectrofotometria Atômica/métodos , Oligoelementos/análise , Minerais/análise
13.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(9): 2559-61, 2012 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-23240438

RESUMO

ICP-AES technique was used to determine the content, accumulation and proportion of mineral elements in L. japonica and analyze the differences after treatment by COR and Me-JA. The results show that: (1) The content of K, Mg, Na, Zn, B and Si in L. japonica were increased by 5.82%, 2.55%, 155.17%, 5.34%, 16.11% and 142.15% respectively after treatment by COR, while the content of P, Ca, Fe and Mn was reduced by 3.99%, 19.20%, 38.89% and 35.96% (2) After treatment by Me-JA, the content of K, Na, Zn, B, and Si were increased by 4.46%, 137.93%, 6.09%, 10.09% and 89.24%, however the content of P, Ca, Mg, Fe and Mn were decreased by 9.82%, 20.29%, 8.49%, 42.00% and 36.80% respectively. (3) When treated by COR and Me-JA, K:P and Na:Zn were increased, while Ca:Mg and Fe:Mn and B:Si were decreased. The results of this study define the influence of COR and Me-JA on the mineral elements of L. japonica, and provide a scientific basis for the rational use of plant growth regulators as well.


Assuntos
Acetatos/química , Ciclopentanos/química , Minerais/análise , Oxilipinas/química , Plantas/química , Espectrofotometria Atômica
14.
Se Pu ; 30(2): 211-4, 2012 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-22679839

RESUMO

A high performance liquid chromatographic (HPLC) method was developed to determine the contents of seven compounds in Lonicera japonica and Folium Lonicerae with maximum wavelength conversion program, and analyze the content differences between them. The separation was performed on an Agilent Eclipse Plus C18 column (250 mm x 4.6 mm, 5 microm) operated at normal temperature with the gradient elution by two mobile phases of water (including 0.3% (v/v) formic acid) (A) and acetonitrile (B) at a flow rate of 1 mL/min. The maximum detection wavelength was set at 330 nm and 350 nm by conversion. The contents of chlorogenic acid and caffeic acid in Lonicera new leaves were 2.572% and 1.498 per thousand respectively, both higher than those cited in Chinese Pharmacopoeia, indicating that Lonicera new leaves have the necessity to be further studied and developed. This method is simple, rapid and highly sensitive. It is suitable for the simultaneous determination of the seven compounds and the quality control of Lonicera japonica.


Assuntos
Ácidos Cafeicos/análise , Ácido Clorogênico/análise , Cromatografia Líquida de Alta Pressão/métodos , Lonicera/química , Ácidos Cafeicos/isolamento & purificação , Ácido Clorogênico/isolamento & purificação , Extratos Vegetais/análise , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Rutina/análise , Rutina/isolamento & purificação
15.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(2): 529-31, 2012 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-22512204

RESUMO

UNLABELLED: ICP-AES technique was used to determine the mineral elements in Bupleurum at different habitat. The results show that: (1) In Bupleurum, the content and accumulation of K was the highest among 5 macroelements, the content and accumulation of Fe was the highest among 5 microelements. (2) In Bupleurum, the content of Ca, Mg, P, Na and Cu was high in habitat of Beijing, the content can respectively reach to 6.40, 3.84, 3.45, 4.97 mg x g(-1), and 25.20 microg x g(-1); while the content of K, Ca, Mg, P, Zn, Mn and Cu was low in habitat of Wanrong,and the content was only 12.43, 4.57, 1.92, 1.79 mg x g(-1) and 50.04, 32.21, 15.43 microg x g respectively. (3) In Bupleurum, the content of P : K, Zn : Fe, Cu and Mn was significantly different at different habitat, while Mg and Ca showed little difference. CONCLUSION: In Bupleurum, the content, accumulation and proportion of mineral elements were difference at different habitat.


Assuntos
Bupleurum , Minerais , Espectrofotometria Atômica , Ecossistema
16.
J Pharmacol Sci ; 118(1): 99-108, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22186624

RESUMO

Cistanche deserticola MA (C. deserticola) has been widely used as a laxative herbal in herbal medicine for the treatment of irritable bowel syndrome or constipation, and echinacoside (ECH) is one of the major bioactive ingredients in this herbal. Our aim was to investigate the effect of ECH on intestinal epithelial cell growth and death. MODE-K, an intestinal epithelial cell line, was used as an in vitro model of the intestine. Cell proliferation was measured by methylthiazol tetrazolium (MTT) assay. Cell apoptosis was determined with Annexin-V staining. Here we showed that in cultured MODE-K cells, ECH significantly stimulated cell proliferation and enhanced cell survival by reducing cell apoptosis in the presence of H(2)O(2) or the mixture of pro-inflammatory cytokines, while transforming growth factor (TGF)-ß1 expression was up-regulated in a dose-dependent manner. Knockdown of TGF-ß1 expression disrupted both the proliferative and cytoprotective activities of ECH, which was further confirmed by neutralization of TGF-ß1 activity using anti-TGF-ß1 antibody. These data suggest that ECH as one of bioactive ingredients in herbal C. deserticola and others may improve mucosal tissue repair by stimulating intestinal epithelial cell proliferation and preventing cell death via up-regulation of TGF-ß.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Células Epiteliais/efeitos dos fármacos , Glicosídeos/farmacologia , Mucosa Intestinal/citologia , Fator de Crescimento Transformador beta1/biossíntese , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Cistanche , Células Epiteliais/metabolismo , Técnicas de Silenciamento de Genes , Camundongos , RNA Mensageiro/metabolismo , Fator de Crescimento Transformador beta1/genética , Regulação para Cima
17.
Phytother Res ; 26(6): 812-9, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22072545

RESUMO

Cistanche deserticola has commonly been used in traditional Chinese medicine to treat many health problems including irritable bowel syndrome or constipation. This study was designed to test the efficacy of a water-extract of C. deserticola in the prevention of colorectal cancer in a mouse model. Polysaccharide-rich water-extract of C. deserticola was prepared by boiling its stem powder in distilled water. Tgfb1Rag2 null mice were used as an experimental model. Here we showed that feeding of water-extract of C. deserticola significantly reduced the number of mucosal hyperplasia and intestinal helicobacter infection in mice. This beneficial effect correlated with significant stimulation of the immune system, evidenced by the enlargement of the spleens with increased number of splenic macrophage and natural killer cells, and with more potent cytotoxicity of splenocytes. In vitro water-extract of C. deserticola enhanced the cytotoxicity of naïve splenocytes against a human colon cancer cell line, and in macrophage cultures up-regulated nitric oxide synthase II expression and stimulated phagocytosis. In conclusion, our data indicate that oral administration of C. deserticola extract reduces inflammatory hyperplastic polyps and helicobacter infection in mice by its immune-stimulatory activity, suggesting that C. deserticola extract may have potential in preventing intestinal inflammation disorders including colorectal cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Cistanche/química , Neoplasias do Colo/tratamento farmacológico , Hiperplasia/tratamento farmacológico , Fitoterapia , Extratos Vegetais/farmacologia , Animais , Antineoplásicos Fitogênicos/química , Contagem de Células , Linhagem Celular Tumoral , Neoplasias do Colo/imunologia , Neoplasias do Colo/microbiologia , Ensaios de Seleção de Medicamentos Antitumorais , Infecções por Helicobacter/tratamento farmacológico , Infecções por Helicobacter/imunologia , Infecções por Helicobacter/microbiologia , Helicobacter hepaticus/imunologia , Helicobacter hepaticus/patogenicidade , Humanos , Hiperplasia/imunologia , Hiperplasia/microbiologia , Células Matadoras Naturais/imunologia , Macrófagos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neoplasias Experimentais , Óxido Nítrico Sintase Tipo II/imunologia , Óxido Nítrico Sintase Tipo II/metabolismo , Fagocitose , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Caules de Planta/química , Polissacarídeos/química , Baço/efeitos dos fármacos , Baço/imunologia , Água
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2824-7, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23285895

RESUMO

The contents of mineral elements in Cistanche tubulosa from different areas and in the soil in which they grew were determined by ICP-AES The results showed that: (1) the contents of K, P, Ca, Mg, Na, Fe, Mn, Zn and B were rich among different samples collected in five locations. (2) the concentrations of 5 macroelements were high values, in which the content of K was the highest in different aeras. the content of Fe was higher than other microelements and specilally, the Fe content from Xinjiang sample reached to 433.56 microg x g(-1). (3) the mineral elements absorption rate of Cistanche tubulosa is different in different areas and the absorption ability of K, Na and P was higher than other elements in Cistanche tubulosa. (4) the concentrations and utilization rate of mineral elements in Cistanche tubulosa from Xinjiang was higher than other areas. The results maybe provided scientific data and suggestion for the quality control of Cistanche tubulosa.


Assuntos
Cistanche/química , Minerais/análise , Solo/química , Espectrofotometria Atômica , China , Ferro/análise , Potássio/análise , Especificidade da Espécie
19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2828-30, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23285896

RESUMO

ICP-AES technique was used to analyse the mineral elements in different organs of Chrysanthemum indicum L. The results show that: (1) The content of K in root, stem, leaves and flower was the highest among macroelements, and can respectively reach to 15.84, 17.74, 31.52 and 37.55 mg x g(-1), while the content of Fe was the highest in microelements in root, stem, leaves and flower, and can respectively reach to 3 219.90, 433.36, 1 519.46, and 1 426.63 microg x g(-1). (2) The accumulation of K, P, Ca, Mg, Mn, Zn and Mo was highest in stem of Chrysanthemum indicum L, and can respectively reach to 240.61, 19.67, 74.87, 18.31 mg x plant(-1) and 893.00, 1 039.08, 2.85 microg x plant(-1), while the accumulation of Na, Fe and Cu was highest in root, and can respectively reach to 11.51 mg x plant(-1), 11 725.27 microg x plant(-1), and 235.24 microg x plant(-1). (3) The proportion of K:P, Ca:Mg, Fe:Mn, Zn:Cu was varied in different organs. The results of the study pinpoint the mineral elements in different organs of Chrysanthemum indicum L. and will be a scientific basis for fertilization.


Assuntos
Chrysanthemum/química , Minerais/análise , Espectrofotometria Atômica , Cálcio/análise , Flores/química , Fósforo/análise , Raízes de Plantas/química , Caules de Planta/química , Potássio/análise , Espectrofotometria Atômica/métodos
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(10): 2831-3, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23285897

RESUMO

Mineral elements content,absoption proportion and requirement of Sophora alopecuroides L. at different stages were studied by ICP-AES technology. The results showed that (1) During mature period, the average proportion for N:K:Ca: Mg:P:Na was 11.91:8.27:7.54:1.02:1.00, and the average proportion for Fe:Zn:B:Cu:Mn was 28.91:2.12:1.40:1.18:1.00; (2) Mineral elements content of every part differs in each period, and stems at vegetative growth stage and seeds at mature period have the highest mineral elements content proportions of the whole plant which were about 45.6% and 36.7% respectively; (3) Sophora alopecuroides L. plants at vegetative growth stage have the largest fertilizer requirement, followed by the flowering period, and the smallest during pod period.


Assuntos
Minerais/análise , Sophora/química , Espectrofotometria Atômica , Oligoelementos/análise , Sementes/química , Sophora/crescimento & desenvolvimento
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