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1.
Chemosphere ; 286(Pt 2): 131798, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34365175

RESUMO

Facile fractionation of lignocellulosic waste into useable forms is essential to achieve a multi-product treatment process especially when the resulting lignin streams are expected for high-value materials valorization. Despite acidic/alkaline deep eutectic solvents (DESs) are promising solvents for lignocellulosic waste fractionation, there is little information about their differences in the fractionation and lignin extraction profiles. In this work, four DESs that were cataloged to acidic types (formic acid-choline chloride, lactic acid-choline chloride) and alkaline types (monoethanolamine-choline chloride, glycerol-K2CO3) were investigated to compare their abilities of bamboo waste fractionation. Physicochemical properties of these resulting cellulose, lignin and derived lignin nanospheres (LNPs) were also assessed. Results showed that DESs could selectively extract lignin via cleaving lignin-carbohydrate linkages and lignin ether bonds. Acidic DESs pretreatments were more effective in biomass delignification (~95.0 %), while alkaline DESs showed better polysaccharide retention. Glycerol-K2CO3 LNPs exhibited much smaller sphere size (50-100 nm) while acidic DESs LNPs showed higher thermal stability due to higher extent of lignin condensation. In addition, MEA-ChCl could introduce amine groups onto lignin hydroxyl. This work provided insightful information for tailoring technique routes to selective lignocellulosic waste fractionation, while facilitating the downstream applications of the obtained cellulose/lignin.


Assuntos
Lignina , Nanosferas , Biomassa , Solventes
2.
Food Chem ; : 131614, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34848091

RESUMO

As the major byproduct of meat processing, bovine bone are produced in large amounts annually. However, the inefficient utilization with low-added value resulted in serious resource waste. The study aims to prepare high-value bovine bone power (BBP) via instant catapult steam-explosion (ICSE) treatment, taking ball milling (BM) method as control. Results showed that ICSE treatment deconstructed bovine bone with more holes emerging, and effectively promoted mineral dissolution and protein degradation while reduced energy consumption. Compared with BM-BBP, ICSE-BBP possessed more protein and essential minerals, presenting in regular elliptical shapes with narrow distribution of particle size (0.1 âˆ¼ 40 µm), and owned better solution stability and protein solubility. ICSE-BBP also exhibited higher mineral release and protein digestibility during GI digestion while revealed no obvious cytotoxicity, indicating the potential applicability in nutrition-fortified foods. Taken together, ICSE technology holds promise in reusing bovine bone, providing an efficient and eco-friendly process for BBP industrial production.

3.
Zhongguo Zhong Yao Za Zhi ; 46(20): 5362-5371, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34738440

RESUMO

The present study explored the effects and its underlying mechanisms of four active fractions of Camellia nitidissima(leaf polyphenols, leaf saponins, flower polyphenols, and flower saponins in C. nitidissima) in inhibiting the proliferation and migration of non-small cell lung cancer(NSCLC) by suppressing the epidermal growth factor receptor(EGFR). MTT assay was used to detect the effect of four active fractions on the proliferation of NCI-H1975 and HCC827 cells. Wound healing assay and Transwell assay were adopted to evaluate the effect of four active fractions on the migration of NSCLC. The effect of four active fractions on the enzyme activity of EGFR was detected. Molecular docking was carried out to explore the direct action capacity and action sites between representative components of the four active fractions and EGPR. Western blot assay was employed to investigate the effect of four active fractions on the protein expression in EGFR downstream signaling pathways. The results of the MTT assay indicated that the cell viability of NCI-H1975 and HCC827 cells was significantly inhibited by four active fractions at 50, 100, 150, and 200 µg·mL~(-1) in a dose-dependent manner. Wound healing assay and Transwell assay revealed that the migration of NCI-H1975 and HCC827 cells was significantly suppressed by four active fractions. In addition, the results of the protein activity assay showed that the enzyme activity of EGFR was significantly inhibited by four active fractions. The molecular docking results confirmed that various components in four active fractions possessed strong binding activity to EGFR enzymes. Western blot assay revealed that four active fractions down-regulated the protein expression of EGFR and its downstream signaling pathways. It is concluded that the four active fractions of C. nitidissima can inhibit NSCLC. The mechanism may be related to EGFR and its downstream signaling pathways. This study provides a new scientific basis for the clinical treatment of NSCLC with active fractions of C. nitidissima, which is of reference significance for further research on the anti-tumor mechanism of C. nitidissima.


Assuntos
Camellia , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Apoptose , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Linhagem Celular Tumoral , Proliferação de Células , Receptores ErbB/genética , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Simulação de Acoplamento Molecular
4.
Artigo em Inglês | MEDLINE | ID: mdl-34744066

RESUMO

BACKGROUND: Exercise training after lumbar fusion surgery (LFS) is important for regaining the strength in the spinal muscles, pain management, and minimizing dysfunction. It may be prudent to evaluate technologies such as web-based chat and social media apps for increasing the efficacy of post-surgery interventions in LFS patients. OBJECTIVE: To explore the effectiveness of a WeChat-based individualized post-discharge rehabilitation program in patients with LFS. METHODS: Seventy-two eligible discharged LFS patients were enrolled from October 2018 to February 2019. The experimental group (36 cases) received a 10-week WeChat-based individualized rehabilitation program, while the control group (36 cases) received routine follow-up guidance. The outcomes were measured using the Exercise Compliance Questionnaire, Numerical Rating Scale, Oswestry Disability Index and Chinese version of the self-efficacy for exercise scale. RESULTS: The analysis using generalized estimation equations method shows significant differences in the interaction effect of group*time in exercise compliance (Wald c2= 7.459, P< 0.05), group effect in pain (Wald c2= 5.811, P< 0.05) and self-efficacy (Wald c2= 16.383, P< 0.05). However, there was no significant difference between the experimental and control groups in the group effect in dysfunction improvement (Wald c2= 2.289, P> 0.05). CONCLUSIONS: The WeChat-based rehabilitation intervention can improve exercise compliance and self-efficacy, and help achieve greater pain relief compared to the routine intervention. However, the WeChat-based intervention did not offer better improvement in the self-dysfunction in the post-discharge LFS patients.

5.
Carbohydr Polym ; 274: 118656, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34702475

RESUMO

In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions. This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area. Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar. LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films. The potential inhibition mechanism was physical barrier and non-productive binding of LNPs. The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE. While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.

6.
Biomed Pharmacother ; 144: 112329, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34653759

RESUMO

Lipid metabolic disorder occurs when ApoE gene is deficient. However, the role of Docosahexaenoic acid (DHA) in relieving hepatic lipid metabolic disorder in apolipoprotein E-deficient (ApoE -/-) mice remains unknown. We fed 3-month-old C57BL/6J wild-type (C57 wt) and ApoE -/- mice respectively with normal or DHA fortified diet for 5 months. We found ApoE gene deficiency caused hepatic lipid deposition and increased lipid levels in plasma and liver. Hepatic gene expression of SRB1, CD36 and FABP5 in ApoE -/- mice, protein expression of HMGCR, LRP1 in C57 wt mice and protein expression of LRP1 in ApoE -/- mice increased after DHA intervention. In DHA-fed ApoE -/- mice, LXRα/ß and PPARα protein expression down-regulated in cytoplasm, but LXRα/ß protein expression up-regulated in nucleus. DHA treatment decreased RXRα and RXRß expression in C57 wt and ApoE -/- female mice. Deletion of ApoE gene caused lipid metabolism disorder in liver of mice. DHA treatment efficiently meliorated lipid metabolism caused by ApoE deficient through the regulation of gene and protein expressions of molecules involved in liver fatty acids transport and lipid metabolism.

7.
J Cell Mol Med ; 25(22): 10736-10746, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34697898

RESUMO

The present study aimed to explore the potential hub genes and pathways of ischaemic cardiomyopathy (ICM) and to investigate the possible associated mechanisms. Two microarray data sets (GSE5406 and GSE57338) were downloaded from the Gene Expression Omnibus (GEO) database. The limma package was used to analyse the differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, Disease Ontology (DO) and Gene Ontology (GO) annotation analyses were performed. A protein-protein interaction (PPI) network was set up using Cytoscape software. Significant modules and hub genes were identified by the Molecular Complex Detection (MCODE) app. Then, further functional validation of hub genes in other microarrays and survival analysis were performed to judge the prognosis. A total of 1065 genes were matched, with an adjusted p < 0.05, and 17 were upregulated and 25 were downregulated with|log2 (fold change)|≥1.2. After removing the lengthy entries, GO identified 12 items, and 8 pathways were enriched at adjusted p < 0.05 (false discovery rate, FDR set at <0.05). Three modules with a score >8 after MCODE analysis and MYH6 were ultimately identified. When validated in GSE23561, MYH6 expression was lower in patients with CAD than in healthy controls (p < 0.05). GSE60993 data suggested that MYH6 expression was also lower in AMI patients (p < 0.05). In the GSE59867 data set, MYH6 expression was lower in CAD patients than in AMI patients and lower in heart failure (HF) patients than in non-HF patients. However, there was no difference at different periods within half a year, and HF was increased when MYH6 expression was low (p < 0.05-0.01). We performed an integrated analysis and validation and found that MYH6 expression was closely related to ICM and HF. However, whether this marker can be used as a predictor in blood samples needs further experimental verification.

8.
Mol Psychiatry ; 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34561613

RESUMO

The spreading of misfolded alpha-synuclein (α-syn) protein has been observed in animal models of Parkinson's disease (PD) and other α-synucleinopathies that mimic human PD pathologies. In animal models, the spreading of α-syn has been associated with motor dysfunction and neuronal death. However, variability in both susceptible brain regions and cellular populations limits our understanding of the consequences of α-syn spreading and the development of associated therapies. Here, we have reviewed the physiological and pathological functions of α-syn and summarized the susceptible brain regions and cell types identified from human postmortem studies and exogenous α-syn injection-based animal models. We have reviewed the methods for inducing α-syn aggregation, the specific hosts, the inoculation sites, the routes of propagation, and other experimental settings that may affect the spreading pattern of α-syn, as reported in current studies. Understanding the spread of α-syn to produce a consistent PD animal model is vital for future drug discovery.

9.
Nat Commun ; 12(1): 5267, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34489437

RESUMO

Na-ion cathode materials operating at high voltage with a stable cycling behavior are needed to develop future high-energy Na-ion cells. However, the irreversible oxygen redox reaction at the high-voltage region in sodium layered cathode materials generates structural instability and poor capacity retention upon cycling. Here, we report a doping strategy by incorporating light-weight boron into the cathode active material lattice to decrease the irreversible oxygen oxidation at high voltages (i.e., >4.0 V vs. Na+/Na). The presence of covalent B-O bonds and the negative charges of the oxygen atoms ensures a robust ligand framework for the NaLi1/9Ni2/9Fe2/9Mn4/9O2 cathode material while mitigating the excessive oxidation of oxygen for charge compensation and avoiding irreversible structural changes during cell operation. The B-doped cathode material promotes reversible transition metal redox reaction enabling a room-temperature capacity of 160.5 mAh g-1 at 25 mA g-1 and capacity retention of 82.8% after 200 cycles at 250 mA g-1. A 71.28 mAh single-coated lab-scale Na-ion pouch cell comprising a pre-sodiated hard carbon-based anode and B-doped cathode material is also reported as proof of concept.

10.
Res Vet Sci ; 140: 233-241, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34534905

RESUMO

In modern poultry production, stress-induced immunosuppression leads to serious economic losses and harm to animals, but the molecular mechanisms governing the effects of stress on the chicken thymus have not been elucidated. In this study, we successfully constructed a stress model of 7-day-old Gushi chickens by adding exogenous corticosterone (CORT) to their diet and determined the microRNA (miRNA) expression profile of thymus tissues using RNA-seq technology. The results identified 51 differentially expressed miRNAs (DEMs), including 30 upregulated miRNAs and 21 downregulated miRNAs. A total of 164 target genes of the DEMs were predicted based on bioinformatic analysis methods, and Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of these target genes were performed. The results from the GO enrichment analysis of the target genes identified 349 significantly enriched terms, including terms associated with the stress response and immune function that are primarily involved in the negative regulation of phagocytosis, the response to stress and the cellular response to stimulus. The KEGG pathway analysis indicated that the enriched pathways related to immunity or stress included the MAPK signaling pathway, lysosomes, endocytosis, and the RIG-I-like receptor signaling pathway. Among these pathways, DEMs (such as gga-miR-2954, gga-miR-106-5p, and gga-miR-16-5p) and corresponding target genes (such as IL11Ra, SIKE1, and CX3CL1) might be strongly correlated with thymic immunity in chickens. The results of this study provide a reference for further research on the molecular regulatory mechanisms governing the effect of stress on the immune function of the chicken thymus.


Assuntos
Galinhas , MicroRNAs , Animais , Galinhas/genética , Perfilação da Expressão Gênica/veterinária , Imunidade , MicroRNAs/genética , Transcriptoma
11.
J Proteomics ; 248: 104353, 2021 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-34418580

RESUMO

Stress-induced immunosuppression is one of the most widespread problems in the poultry industry. Understanding the molecular regulatory mechanism of immunosuppression induced by stress in the chicken spleen would provide a scientific foundation for the prevention of stress reactions and antistress molecular breeding in poultry. To assess the protein expression profile of spleen tissue in a stress-included immunosuppression model, we performed a TMT-based proteomic analysis of chicken spleen tissue in a Dex-induced immunosuppression model (group C) and a control group (group A). We identified 590 differentially abundant proteins (DAPs) in chicken spleen tissue. These DAPs were significantly enriched in the following functional categories: ECM-receptor interaction, DNA replication, p53 signaling pathway, PI3K-Akt signaling pathway and NF-kappa B signaling pathway. Integrative analysis of the proteome and our previous transcriptome data revealed 62 DAPs showing correlations with the expression of their encoding mRNAs. Complementary proteome- and transcriptome-level analyses revealed a complex molecular network of stress-included immunosuppression. DPP4 and ALDH1A3 were the most significantly upregulated DAPs. GBP and OASL were identified as important nodes in the network related to stress-induced immunosuppression. The candidate genes identified in this study may be useful for the marker-based breeding of new chicken varieties with reduced stress levels. SIGNIFICANCE: This study provides a large amount of new information about the spleen proteome of the Dex-induced immunosuppression in chicks, as well as the correlation of transcriptome and proteome. Analysis of this resource has enabled us to examine mechanism of protein and transcript diversification, which expands the understanding of the complexity of the mechanism of stress-induced immunosuppression.


Assuntos
Galinhas , Proteômica , Animais , Dexametasona/farmacologia , Imunossupressão , Fosfatidilinositol 3-Quinases , Baço , Transcriptoma
12.
J Ethnopharmacol ; 279: 114398, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34242729

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Folium Artemisiae Argyi (FAA) is one kind of Chinese herbal medicine with a long history. It has widespread pharmacological activities such as antibacterial, anti-inflammatory, antioxidative, and hemostatic, among others. FAA is traditionally used for the treatment of eczema, respiratory diseases and gynecological diseases for hundreds of years. Flavonoids are reported as the main components of them. Recent studies focused on the antioxidant effect of its flavonoids in vitro, while few studies focused on the antioxidant effect in vivo, and the underlying mechanisms have not yet been elucidated. AIM OF THE STUDY: The aim of this study was to evaluate the antioxidant activity of Folium Artemisia Argyi flavonoids (FAAF) and explore its possible molecular mechanism in Caenorhabditis elegans. The research and development of its medicinal value will beneficial to the resource utilization of FAA. MATERIALS AND METHODS: Firstly, FAAF was prepared, purified and then qualitatively and quantitatively analyzed using LC-DAD-MS. Then, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), hydroxyl radical and ferric reducing antioxidant power (FRAP) assays were applied to investigate the antioxidant effect of FAAF in vitro. Meanwhile, a stress resistance assay was carried out to evaluate the antioxidant effect of FAAF in vivo. Moreover, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and reactive oxygen species (ROS) accumulation were determined to ascertain whether FAAF can increase the oxidant defense system of nematodes and reduce the accumulation of ROS. Lipofuscin and protein carbonylation assays were employed to test whether FAAF can increase the antioxidant capacity of nematodes to resist the growth health indicators related to antioxidation. At last, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate the expression of genes related to antioxidation. The expression of green fluorescent protein (GFP) was further investigated using a fluorescence microscope in transgenic strains (SOD-3::GFP, GST-4::GFP, and HSP-16.2::GFP). RESULTS: FAAF exhibited a strong antioxidant capacity and enhanced stress resistance in C. elegans. FAAF reduced ROS accumulation and improved the antioxidant defense system under acute stress. Moreover, FAAF prevented the accumulation of lipofuscin and protein carbonylation in C. elegans. FAAF also upregulated the gene expression levels of hsp-16.2, gst-4, sod-3, skn-1, daf-16, ctl-2, hsf-1 and increased SOD-3::GFP and GST-4::GFP expression. CONCLUSION: These results demonstrated that FAAF exerted antioxidant activity in C. elegans. It was perhaps regulated by the insulin/insulin-like growth factor-1(IGF-1) signaling pathway.

13.
Cancer Invest ; 39(9): 741-755, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34229540

RESUMO

To explore the expression profile and prognostic relevance of GLUT-1 in pancreatic cancer, a meta-analysis, bioinformatics analysis based on Gene Expression Omnibus (GEO), Oncomine dataset and The Cancer Genome Atlas (TCGA) database, and immunohistochemistry in tumor and normal tissue from 88 pancreatic ductal adenocarcinoma (PDAC) patients were performed. GLUT-1 was significantly overexpressed in pancreatic cancer but it could not be a significant biomarker for prognosis. TNM stage and pathological grade could be biomarker of poor prognosis of patients with pancreatic cancer.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma Ductal Pancreático/genética , Biologia Computacional/métodos , Regulação Neoplásica da Expressão Gênica , Transportador de Glucose Tipo 1/genética , Neoplasias Pancreáticas/genética , Idoso , Biomarcadores Tumorais/metabolismo , Carcinoma Ductal Pancreático/metabolismo , Carcinoma Ductal Pancreático/terapia , Feminino , Perfilação da Expressão Gênica/métodos , Transportador de Glucose Tipo 1/metabolismo , Humanos , Imuno-Histoquímica/métodos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Neoplasias Pancreáticas/metabolismo , Neoplasias Pancreáticas/terapia , Prognóstico , Estudos Retrospectivos
14.
J Med Virol ; 93(11): 6163-6171, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34260072

RESUMO

Borna disease virus (BoDV-1) can infect the hippocampus and limbic lobes of newborn rodents, causing cognitive deficits and abnormal behavior. Studies have found that neuroinflammation caused by viral infection in early life can affect brain development and impair learning and memory function, revealing the important role of neuroinflammation in cognitive impairment caused by viral infection. However, there is no research to explore the pathogenic mechanism of BoDV-1 in cognition from the direction of neuroinflammation. We established a BoDV-1 infection model in rats, and tested the learning and memory impairment by Morris water maze (MWM) experiment. RNAseq was introduced to detect changes in the gene expression profile of BoDV-1 infection, focusing on inflammation factors and related signaling pathways. BoDV-1 infection impairs the learning and memory of Sprague-Dawley rats in the MWM test and increases the expression of inflammatory cytokines in the hippocampus. RNAseq analysis found 986 differentially expressed genes (DEGs), of which 845 genes were upregulated and 141 genes were downregulated, and 28 genes were found to be enriched in the toll-like receptor (TLR) pathway. The expression of TLR4, MyD88, and IRF5 in the hippocampus was significantly changed in the BoDV-1 group. Our results indicate that BoDV-1 infection stimulates TLR4/MyD88/IRF5 pathway activation, causing the release of downstream inflammatory factors, which leads to neuroinflammation in rats. Neuroinflammation may play a significant role in learning and memory impairment caused by BoDV-1 infection.

15.
J Hum Evol ; 157: 103033, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34246050

RESUMO

In contrast to the prevailing view that the Chinese Paleolithic has been dominated by the Mode 1 technology-with a slow and conservative development from the Early to the Late Pleistocene-recent discoveries indicate that the lithic technology might have developed into an 'advanced' phase in some parts of China, at least since the early Late Pleistocene. The Xinmiaozhuang Locality 1 (XMZ1), located on the southern edge of the Nihewan Basin in northern China, is one of the examples belonging to such an 'advanced' phase. Although the stone artifacts at this site still belong to the long-existing 'small-tool' industry (core-and-flake) in this basin, some 'advanced' traits, including discoidal cores, elongated flakes, and 'Mousterian-like' triangular points and scrapers, are present. We provide a dating of the XMZ1 using the multiple elevated temperatures (MET) post infrared (pIR) infrared stimulated luminescence (IRSL) procedure (MET-pIRIR) on both multigrained single aliquots and 'individual' grains of potassium-rich feldspars (K-feldspars). The consistency between the single-aliquot and single-grain K-feldspar equivalent dose results mutually confirmed the reliability of the obtained ages. Our chronology indicates that the cultural layer falls within the period of ca. 63-75 ka, corresponding to the early stage of the Marine Isotope Stage (MIS) 4. Based on the correlation of the cultural age to the environmental proxies of loess and stalagmites from China, we suggest that the site might have witnessed dramatic fluctuations of paleoclimate during the site formation. Additionally, based on the discoidal cores distribution, a potential corridor along the Xuefeng-Wu-Tainhang-Great Khingan Mountains for ancient humans migrating between South and North China is suggested. However, more archaeological and chronological studies are required to figure out the origin and the dispersal patterns of the discoidal core associated with lithic assemblage and the tool-makers in East Asia.


Assuntos
Arqueologia , Meio Ambiente , Luminescência , China , Fósseis , História Antiga , Humanos , Reprodutibilidade dos Testes , Tecnologia/história , Comportamento de Utilização de Ferramentas
16.
Clin Rehabil ; 35(12): 1674-1693, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34227435

RESUMO

OBJECTIVE: With the increasing incidence and earlier onset of cancer, more and more cancer patients are facing the problems of return-to-work. This review is to explore the types, contents, and results of return-to-work interventions for cancer patients. METHODS: This scoping review followed Arksey and O'Malley's framework and PRISMA-ScR List. Three Chinese databases and five English databases were searched from the establishment of databases to 31 March, 2021. Article selection and data extraction were conducted by two researchers. RESULTS: Thirty-two studies and 1916 cancer patients with mainly breast and gastrointestinal cancer were included. According to the contents, interventions could be divided into four types: (1) physical interventions (n = 6), including high-intensity exercise, low-to-moderate intensity exercise, yoga, and upper limb functional training, (2) psychological interventions (n = 2), including early active individualized psychosocial support and mindfulness-based recovery, (3) vocational interventions (n = 14), including making work plans, educational leaflets, vocational consultations, electronic health intervention, and interventions targeting at employers, (4) multidisciplinary interventions (n = 10), including any combination of above interventions. Physical exercises, making working plans, vocational consultations, educational leaflets, two combinations of vocational and physical interventions were validated to have positive results in enhancing cancer patients' return-to-work. CONCLUSIONS: Return-to-work interventions for cancer patients are diversified and can be divided into physical, psychological, vocational, and multidisciplinary interventions. Medical staffs can utilize physical exercises, making working plans, vocational consultation, educational leaflets, combinations of vocational and physical interventions to enhance cancer patients' return-to-work. Other interventions still need to be developed and validated.


Assuntos
Neoplasias , Retorno ao Trabalho , Exercício Físico , Humanos , Modalidades de Fisioterapia , Reabilitação Vocacional
17.
J Neurochem ; 159(3): 414-416, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34296424

RESUMO

Iron accumulation and α-synuclein aggregates (e.g., Lewy bodies) have been linked with the pathogenesis of Parkinson's disease (PD), with yet-to-be-determined interaction. Previous studies have indicated that iron binds to α-synuclein and triggers its aggregation in vitro, and iron is found enriched in Lewy bodies. In the current study, Joppe et al. have found that the propagation of pathological α-synuclein caused by intrastriatal α-synuclein preformed fibrils (PFFs) injection was unexpectedly attenuated in rodent brains in a model of brain iron elevation (neonatal iron feeding). PFFs stimulated microglial activation was also reduced in mice with elevated iron. These results may provide new insight into the complex interaction between these two key pathologies of PD.


Assuntos
Doença de Parkinson , alfa-Sinucleína , Animais , Encéfalo/metabolismo , Ferro , Corpos de Lewy/metabolismo , Camundongos , alfa-Sinucleína/metabolismo
18.
Food Res Int ; 145: 110401, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34112404

RESUMO

As a kind of promising resource, animal bone has been widely processed into functional foods. However, there is little research about the effect of particle size on the physicochemical properties and digestibility of yak bone powder (YBP), as well as its anti-osteoporosis activity. In this study, the YBP with median particle sizes (MPS) ranging from 19.68 to 128.37 µm were prepared, and their digestibility and anti-osteoporosis activity were investigated. The results showed that smaller MPS significantly increased water holding capacity and protein solubility without changing composition. The MPS reduction greatly promoted protein digestion, producing more peptides<3 kDa and free amino acids while decreased Ca2+ and P5+ release during gastrointestinal digestion. The in vivo results revealed the positive effect of YBP on ovariectomy-induced osteoporosis in rats. The bone mineral density of ovariectomized (OVX) rats was obviously improved by regulating bone turnover markers (B-ALP, OCN, S-CTX, ES and TRAP), thus potentially shedding light on osteoporosis remission. However, different MPS exhibited a weak effect on osteoporosis in OVX rats. Therefore, YBP could be produced in relatively large particle size without sacrificing food sensory quality, the processing time of which could also be shortened for higher productivity and lower cost.


Assuntos
Osteoporose , Animais , Osso e Ossos , Bovinos , Modelos Animais de Doenças , Feminino , Humanos , Ovariectomia , Tamanho da Partícula , Pós , Ratos
19.
BMC Nurs ; 20(1): 113, 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34182971

RESUMO

BACKGROUND: The aging population has become a serious challenge for health care service and will lead to an increasing demand for nurses to work with older people. However, working with older people has always been an unpopular career choice among nursing students. This study aimed to further explore the willingness and associated factors of undergraduate nursing students to work with older people in China. METHODS: A convenience sampling method was employed from May to July 2017 among undergraduate nursing students from a nursing school in Nantong China. Demographic data, the Chinese version of the Kogan's Old Person's Scale, the Chinese version of the Facts of Aging Quiz and the motivation questionnaire were used to collect data. A series of Mann-Whitney U test, Kruskal-Wallis H test, Spearman correlation test and Ordinal logistic regression analysis were applied to analyze the data. RESULTS: Of the 853 students surveyed, 38.1 % were willing to work with older people after graduation. Expectancy, interest, attainment value, cost, prejudice, whether they like nursing profession and whether they participated in elderly-related activities were the most significant predictors of the students' willingness to work with older people. CONCLUSIONS: Nursing students expressed a low level of willingness to work with older people upon graduation. Nursing educators have an important part in challenging students' stereotype of older people and inspire their career choice in caring for older people through both well-designed curriculum and elderly-related activities, and relevant education departments should actively optimize aged-related courses, strengthen professional ethics and gratitude education, and improve nursing students' sense of identity and mission in caring for older people, so as to improve their willingness to work with older people.

20.
Res Vet Sci ; 138: 90-99, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34126450

RESUMO

With the rapid development of the poultry breeding industry and highly intensive production management, the losses caused by stress responses are becoming increasingly serious. To screen candidate genes related to chicken stress and provide a basis for future research on the molecular mechanisms governing the effects of stress on chicken immune function, we successfully constructed a chicken stress model by exogenously introducing corticosterone (CORT). RNA-seq technology was used to identify and analyze the mRNA and enrichment pathways of the thymus in the stress model group and the control group. The results showed that there were 101 significantly differentially expressed genes (SDEGs) (Padj < 0.05, |log2fold changes| ≥ 1 and FPKM >1), of which 44 were upregulated genes, while 57 were downregulated genes. Gene Ontology (GO) enrichment analysis found that the terms related to immunity or stress mainly included antigen processing and presentation, positive regulation of T cell-mediated immunity, and immune effector process. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the main pathways related to immunity or stress were the PPAR signaling pathway, NOD-like receptor signaling pathway, and intestinal immune network for IgA production. Among the SDEGs, XCL1, HSPA8, DMB1 and BAG3 are strongly related to immunity or stress and may be important genes involved in regulating stress affecting the immune function of chickens. The above results provide a theoretical reference for subsequent research on the molecular regulatory mechanisms by which stress affects the immune function of poultry.


Assuntos
Galinhas/genética , Imunidade/genética , Timo/imunologia , Transcriptoma , Animais , Galinhas/imunologia , Distribuição Aleatória , Estresse Fisiológico/imunologia , Timo/metabolismo
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