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1.
Huan Jing Ke Xue ; 40(5): 2287-2294, 2019 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087868

RESUMO

The synergistic process and mechanism of aluminum (Al)-substituted ferrihydrites on arsenic[As(Ⅴ)] and cadmium[Cd(Ⅱ)] were studied under laboratory conditions. The results showed that synergistic adsorption and coprecipitation of As and Cd by Al-substituted ferrihydrites was clearly affected by both the pH of solution and the order in which heavy metals were added. The solution in which As co-existed with Cd for 72 hours, at a pH of 6.0 to 6.5, the As and Cd adsorption capacity of Al-substituted ferrihydrites containing 20% Al (AF20) reached 60.9 mg·g-1 and 17.1 mg·g-1, respectively. The removal rates of As and Cd were 96.0% and 73.0%, respectively. Arsenic and Cd were synergistically adsorbed into the internal pores of AF20 particles, and the synergistic adsorption effect of AF20 on As and Cd was clear. Adding Cd to the solution containing As, for 72 hours, and with a pH of 6.1 to 6.5, the As and Cd adsorption capacity of AF20 was 58.1 mg·g-1 and 12.4 mg·g-1, respectively. The removal rates of As and Cd were 96.0% and 48.3%, respectively. Adsorption of As limited the fixation of Cd by AF20. When adding As to the solution containing Cd, for 72 hours, with a pH of 9.5 to 9.8, fixed amounts of As and Cd on AF20 were 20.9 mg·g-1 and 24.4 mg·g-1, respectively. The removal rates of As and Cd were 38.8% and 98.9%, respectively. The coprecipitation of As and Cd by AF20 was clear. The resulting insoluble As and Cd compounds distributed the Cd distribution in a sparse strip and impeded the further adsorption of As. The results show that Al-substituted ferrihydrites can synergistically adsorb and coprecipitate As and Cd in contaminated environmental media.

2.
Huan Jing Ke Xue ; 39(1): 399-405, 2018 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965707

RESUMO

The objective of this study was to determine the effect of five scenarios on the accumulation of Cd in the soil-rice system, including the return of straw to the field and the lack of the return, atmospheric deposition control, use of clean water for irrigation, and the use of lime. For the field experiments, three typical paddies were selected and divided into five plots (5 m×6 m) in Xiangtan, Zhuzhou, and Liling in the Hunan province from April to October 2016. The results showed that the application of lime can increase pH by 0.87, while the available Cd concentration in the soil was decreased by 33.7%. The accumulations of Cd in roots, stems, and brown rice were decreased by 47.9%, 46.7%, and 54.8%, respectively, with a decrease in the corresponding bioconcentration factors. Irrigating with clean water and liming tended to increase the soil pH by 0.44 and 0.49, respectively, while the available Cd concentration in the soil was decreased by 18.2% and 14.5%, respectively. The Cd concentrations in roots, stems, and brown rice were decreased by 32.6%, 24.2%, and 18.0%, and 17.6%, 11.3%, and 25.4% with decreased bioconcentration factors under both treatments (irrigating with clean water and liming). The available Cd concentration in the soil was increased by 6.1% and the Cd accumulation in the rice plants also increased with the return of straw to the soil. The bioconcentration factors of the rice plants were also increased when the paddy straw was returned to the fields. The results showed that the measures, such as the use of lime, atmospheric deposition control, use of clean water for irrigation, and lack of the return of straw to the paddy soil, should be helpful for the safe production of brown rice. The possible long-term risks associated with returning straw to the paddy field should be evaluated scientifically.


Assuntos
Cádmio/análise , Oryza/química , Poluentes do Solo/análise , Solo/química , Raízes de Plantas/química , Caules de Planta/química
3.
Ecotoxicol Environ Saf ; 159: 38-45, 2018 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-29730407

RESUMO

Agricultural soils contaminated with cadmium (Cd) pose a risk to receiving surface water via drainage or runoff. A 90-day laboratory incubation experiment was conducted to investigate the release characteristics and transformation of Cd from contaminated paddy soil amended with agrochemical (NPK fertilizer) and lime (L) under water management regimes of continuous flooding (F) and drying-wetting cycles (DW). The result showed that the dissolved Cd concentrations in overlying water of the fertilizer treatment under flooding (NPK+F) and drying-wetting (NPK+DW) reached up to 81.0 µg/L and 276 µg/L, and were much higher than that from the corresponding controls without NPK fertilizer addition at the end of experiment. The Cd concentration showed significantly negative correlation with overlying water pH, but positive correlation with soil redox potential and concentrations of dissolved total nitrogen, sulfate and manganese in overlying water (P < 0.05), indicating that drying-wetting cycles and N fertilizer addition may enhance soil Cd release. The Cd concentrations in overlying water from all treatments except NPK+L+F treatment exceeded the Cd threshold limit of Chinese Environmental Quality Standards for Surface Water (10 µg/L Grade V) and poses potential risk to surface water quality. Meanwhile, the proportion of Cd in the acid-soluble fraction from all incubated soil except NPK+L+F treatment increased compared to before incubation. The results indicated that continuous flooding was a reasonable water management candidate coupled with lime addition for immobilizing soil Cd.


Assuntos
Agricultura/métodos , Cádmio/química , Compostos de Cálcio/química , Fertilizantes , Óxidos/química , Poluentes do Solo/química , Poluição da Água/prevenção & controle , Inundações , Nitrogênio/química , Oryza , Fósforo/química , Potássio/química
4.
Huan Jing Ke Xue ; 39(11): 5207-5216, 2018 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628246

RESUMO

A greenhouse experiment was conducted to study the potential of intercropping Arundo donax with Broussonetia papyrifera or Morus alba to remediate heavy metal-contaminated soil. The results showed that intercropping the herbaceous plant A. donax with woody plants B. papyrifera or M. alba was beneficial for plant growth on heavy metal-contaminated soil. This can effectively enhance the comprehensive enrichment capacity of heavy metals and improve soil enzyme activities. The photosynthetic pigment contents in the leaves of A. donax, B. papyrifera, and M. alba decreased along with remediation time under monoculture treatment for each plant. However, compared with 90 d cultivation, the chlorophyll-a and carotenoid contents in B. papyrifera leaves and chlorophyll-b and carotenoid contents in M. alba leaves under intercropping treatment after 270 d cultivation were only slightly changed. Furthermore, chlorophyll-a, chlorophyll-b, and carotenoid contents in M. alba leaves under intercropping treatment were significantly (P<0.05) increased by 99.1%, 177.1%, and 119.9%, respectively, compared with monoculture-treated M. alba, and the total biomass of M. alba increased significantly (P<0.05) by 26.1%. Compared with monoculture-treated A. donax, the total accumulation amounts of Pb and Zn in the shoots of combined plants was significantly (P<0.05) enhanced by 171% and 124% under intercropping treatment of A. donax with B. papyrifera. Compared with monoculture-treated M. alba and A. donax, the total accumulation amounts of As and Pb in the shoots of intercropped plants were significantly (P<0.05) enhanced by 150% and 76.5%, respectively, under intercropping treatment of A. donax with M. alba. Moreover, the fractions of As, Cd, Pb, and Zn in contaminated soil slightly changed under intercropping treatment of A. donax with B. papyrifera or M. alba, and soil urease, acid phosphatase, and total phosphatase activity was superior to part of the monoculture treatments after 270 d cultivation. The results further suggested that intercropping A. donax with B. papyrifera or M. alba could be effectively used for heavy metal-contaminated soil remediation, while simultaneously improving the biological quality in contaminated soil.


Assuntos
Biodegradação Ambiental , Metais Pesados/isolamento & purificação , Poaceae/metabolismo , Poluentes do Solo/isolamento & purificação , Betula/crescimento & desenvolvimento , Morus/crescimento & desenvolvimento , Solo
5.
Medicine (Baltimore) ; 96(14): e6533, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28383418

RESUMO

For Siewert type II adenocarcinoma of the esophagogastric junction (AEJ), the optimal surgical approach and extent of lymph nodes dissection remain controversial. Immunohistochemistry (IHC) has been reported to be available for identifying lymph node micrometastasis (LNMM) in patients with AEJ. This was a prospective case series of patients who underwent R0 resection and lower mediastinal lymphadenectomy from January 2010 to June 2015 in Fujian Medical University Union Hospital for Siewert type II AEJ. The outcomes were analyzed retrospectively. A total of 1325 lymph nodes were collected from 49 patients, grouped into 3 groups: lower mediastinal, paracardial, and abdominal. The former 2 groups were examined by monoclonal antibodies against Ber-Ep4 and CD44v6. The incidence of LNMM in mediastinal group was 37% (18/49) for Ber-Ep4 and 33% (16/49) for CD44v6. While in routine histological diagnosis, the number of patients with the positive lymph nodes was 7 (14%). When combining IHC with histopathology (HE) staining, the incidence of positive mediastinal lymph nodes was increased to 24%, with a total number of 37 lymph nodes from 28 patients (57%). Micrometastases indicated by Ber-Ep4 and CD44v6 were associated with the depth of tumor invasion (P = 0.020 and 0.037, respectively), histopathological nodal status (P = 0.024 and 0.01, respectively), and Lauren classification (P = 0.038 and, respectively). Expression of CD44v6 and Ber-Ep4 was positively correlated (r = 0.643, P < 0.001). The 3- and 5-year survival rates for all patients were 66% and 50%, respectively. The patients with LNMM had a lower 3-year survival rate of 51%, compared to 80% from no LNMM group; 5-year survival rate was also lower in LNMM group, which is 29% versus 68% (P = 0.006) in the no LNMM group. Patients with positive Ber-Ep4 cells had a lower survival, but not statistically significant (P = 0.058). CD44v6-positive group had a significantly reduced survival (P < 0.001). In patients group with negative lower mediastinal lymph nodes, patients without LNMM obtained a significant survival benefit (P = 0.021). Our study demonstrated that routine test for LNMM is necessary for patients with negative lymph nodes. As a positive prognostic factor, thorough lower mediastinal lymphadenectomy in an invasive approach should be considered when necessary. Ber-Ep4 and CD44v6 were shown to be great markers for detecting LNMM.


Assuntos
Biomarcadores Tumorais/análise , Receptores de Hialuronatos/análise , Linfonodos/química , Micrometástase de Neoplasia/diagnóstico , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/cirurgia , Feminino , Humanos , Excisão de Linfonodo , Linfonodos/patologia , Metástase Linfática , Masculino , Mediastino , Pessoa de Meia-Idade , Melhoria de Qualidade , Estudos Retrospectivos , Neoplasias Gástricas/patologia , Neoplasias Gástricas/cirurgia
6.
Ecotoxicol Environ Saf ; 142: 200-206, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28411515

RESUMO

High levels of vanadium (V) have long-term, hazardous impacts on soil ecosystems and biological processes. In the present study, the effects of V on soil enzymatic activities, basal respiration (BR), microbial biomass carbon (MBC), and the microbial community structure were investigated through 12-week greenhouse incubation experiments. The results showed that V content affected soil dehydrogenase activity (DHA), BR, and MBC, while urease activity (UA) was less sensitive to V stress. The average median effective concentration (EC50) thresholds of V were predicted using a log-logistic dose-response model, and they were 362mgV/kg soil for BR and 417mgV/kg soil for DHA. BR and DHA were more sensitive to V addition and could be used as biological indicators for soil V pollution. According to a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, the structural diversity of the microbial community decreased for soil V contents ranged between 254 and 1104mg/kg after 1 week of incubation. As the incubation time increased, the diversity of the soil microbial community structure increased for V contents ranged between 354 and 1104mg/kg, indicating that some new V-tolerant bacterial species might have replicated under these conditions.


Assuntos
Consórcios Microbianos/efeitos dos fármacos , Microbiologia do Solo , Poluentes do Solo/toxicidade , Solo/química , Vanádio/toxicidade , Biomassa , Análise por Conglomerados , Eletroforese em Gel de Gradiente Desnaturante , Ecossistema , Consórcios Microbianos/genética , Consórcios Microbianos/fisiologia , Modelos Teóricos , RNA Ribossômico 16S/genética , Poluentes do Solo/análise , Vanádio/análise
7.
Sci Total Environ ; 576: 292-302, 2017 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-27788444

RESUMO

An orthogonal field experiment of giant reed (Arundo donax) modified with organic complex fertiliser (OCF), and OCF and fly ash (O&F), at different planting densities was carried out in metal-contaminated soil. The available percentage of arsenic (As) and lead (Pb) in soil decreased from 8.45% to 2.19% and from 29.6% to 13.5% by OCF, respectively, and that of cadmium (Cd) was reduced from 25.3% to 6.49% by O&F. The total biomass of giant reed was 631g per individual following application of O&F in contaminated soil. The accumulation of As, Cd, and Pb in giant reed was 1.57, 4.06, and 11.25mg per individual. Urease and sucrase activity were 87.4NH4-Nµg/gd and 63.1glucosemg/gd in response to the treatments modified using OCF, while the highest dehydrogenase activity was 101 TPF (triphenyltetrazolium formazan) µg/gd in the treatments modified using O&F. Dominant bacteria (frequency>50%) were enriched with increasing planting density of giant reed. These results indicate that the phytostabilisation of metal-contaminated soil by giant reed could be improved by the application of O&F or OCF.

8.
Int J Clin Pharmacol Ther ; 54(6): 484-94, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27049053

RESUMO

OBJECTIVE: To establish a developed HPLC-ESI-MS/MS method for simultaneous determination of mesalazine (5-ASA) and its major metabolite N-Ac-5-ASA in human plasma and to investigate bioequivalence of two enteric-coated mesalazine tablets as well as the effect of high-fat food on the pharmacokinetics of 5-ASA and N-Ac-5-ASA. METHODS: In this open-label, randomized, crossover, two-states, four-period study, 20 healthy Chinese volunteers were randomized to receive a single oral dose of trial or reference preparation (2 × 250 mg) under fasting and fed state. Plasma samples were obtained at 0, 1, 2, 3, 4, 4.5, 5, 5.5, 6, 6.5, 7, 8, 10, 12, 24, and 36 hours postdose and were measured by a developed HPLC-ESI-MS/MS method. Safety and tolerability were assessed throughout the study. RESULTS: The HPLC-ESI-MS/MS method required only 7.0 minutes run time and was successfully applied in analyzing ~ 2,000 samples. High-fat-food administration prolonged tmax of 5-ASA and N-Ac-5-ASA (p < 0.05), while AUC was not significantly affected by the meal (p > 0.05). The 90% confidence intervals (CIs) of the fed/fasting and trial/reference ratios of log-transformed Cmax and AUC were within 80-125%. The two one-sided t-tests showed that the trial and reference preparation were bioequivalent (p > 0.05). CONCLUSIONS: This developed HPLC-ESI-MS/MS method is suitable for massive biomedical analysis. Trial and reference preparations are bioequivalent under fasting and fed state. High-fat-food administration delays the absorption of mesalazine while total exposure is not affected. Dietary habits should always be taken into consideration when enteric-coated mesalazine tablets were prescribed to patients.


Assuntos
Interações Alimento-Droga , Mesalamina/farmacocinética , Adulto , Área Sob a Curva , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Voluntários Saudáveis , Humanos , Masculino , Mesalamina/efeitos adversos , Espectrometria de Massas por Ionização por Electrospray , Comprimidos com Revestimento Entérico , Espectrometria de Massas em Tandem , Equivalência Terapêutica
9.
Int J Clin Pharmacol Ther ; 54(6): 455-61, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27049056

RESUMO

BACKGROUND AND PURPOSE: Spironolactone is a potassium-sparing diuretic and is a competitive antagonist of aldosterone, which is widely used in the treatment of primary aldosteronism, essential hypertension, congestive cardiac failure, and various edematous states. The purpose of this study was to compare the pharmacokinetic properties and bioequivalence of the two formulations of spironolactone tablets in healthy Chinese male subjects under fasting and fed condition. METHODS: A total of 40 male subjects were enrolled in this randomized, open-label, two-period crossover study, subjects in 2 groups (20 individuals in each group) received a single 100-mg dose of test or reference spironolactone tablet formulations with a 2-week washout period under both fasting and fed condition. The plasma concentrations of canrenone, a major active metabolite of spironolactone, were quantified by a validated high performance liquid chromatography-tandem mass spectrometry method. The pharmacokinetic parameters including AUC0-tlast, AUC0-∞, tmax, and Cmax were employed to test bioequivalence. RESULTS: The relative bioavailability was 99.2 ± 11.6% and 97.6 ± 7.4% under fasting and fed condition, respectively. The 90% confidence intervals of the adjusted geometric mean ratio (test/reference) of Cmax, AUC0-tlast, and AUC0-∞ were 89.7-113.8%, 93.9-103.3%, and 90.0-103.0% in fasting study and 87.7-102.3%, 95.1-99.5%, and 94.1-98.9% in fed study, respectively. CONCLUSIONS: Based on pharmacokinetic parameters and the Chinese Food and Drug Administration's guidance and regulatory criteria for bioequivalence, the test and reference formulations of spironolactone were bioequivalent under both fasting and fed condition. Both formulations were generally well tolerated, with no adverse reaction reported.


Assuntos
Espironolactona/farmacocinética , Adolescente , Adulto , Estudos Cross-Over , Jejum , Humanos , Masculino , Comprimidos , Equivalência Terapêutica
10.
J Zhejiang Univ Sci B ; 16(6): 524-32, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26055914

RESUMO

The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Suínos/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Especificidade de Órgãos , Suínos/genética , Distribuição Tecidual
11.
Curr Alzheimer Res ; 12(1): 80-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25523422

RESUMO

PURPOSE: To examine hippocampal differences between Alzheimer disease (AD), amnestic mild cognitive impairment (aMCI) and normal aging. MATERIALS AND METHODS: This study was approved by the local ethics review board, and informed consent was obtained from volunteers/guardians. The study comprised 60 subjects: 20 aMCI patients, 20 AD patients and 20 normal controls (NC group). Magnetic resonance imaging and diffusion kurtosis imaging of the hippocampus were performed. Nonlinear fitting routines and equations were used to calculate mean diffusion (MD) and mean kurtosis (MK). Analysis of variance was used to compare the bilateral MK/MD values/volumes of the hippocampus for the three groups using the Fisher least significant difference test (a two-sample t-test comparison). Pearson correlation coefficients were calculated for the correlations between mini-mental state examination (MMSE) scores and MK/MD values/volumes. RESULTS: There were significant differences between the AD and NC groups and between the AD and aMCI groups in terms of normalized bilateral hippocampal volume. There were significant differences between the NC, aMCI and AD groups in terms of both right and left hippocampus MK values. For the right hippocampus MD values, there was a significant difference between the NC and AD groups. For the left hippocampus MD values, there were significant differences between the AD and NC groups and between the AD and aMCI groups. Pearson correlation coefficients for all correlations between MMSE scores and hippocampus MK/MD values/volumes were significant. CONCLUSION: The bilateral hippocampal MK/MD values may be more sensitive than volumes in the diagnosis of aMCI and AD patients.


Assuntos
Envelhecimento/patologia , Doença de Alzheimer/patologia , Disfunção Cognitiva/patologia , Imagem de Difusão por Ressonância Magnética/métodos , Hipocampo/patologia , Idoso , Feminino , Lateralidade Funcional , Humanos , Imageamento Tridimensional , Imagem por Ressonância Magnética , Masculino , Entrevista Psiquiátrica Padronizada , Pessoa de Meia-Idade , Dinâmica não Linear , Estatística como Assunto
12.
Neurol Sci ; 35(12): 1963-7, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25030124

RESUMO

It was previously reported that cytokines and neurotoxins released from activated inflammatory cells induced the loss of projecting dopaminergic neurons in the substantia nigra, which triggered the pathogenesis of PD. The present study investigated the effect of treatment with tetramethylpyrazine (TMP) on the central cytokine synthesis, striatal dopamine content and glutamatergic transmission, and behavioral performance in the rotarod task in mice injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Treatment with TMP significantly improved the behavioral performance in the rotarod task in mice injected with MPTP. It also decreased the upregulation of cytokines (tumor necrosis factor-α and interleukin-1ß) in the substantia nigra and striatum in these modeled mice. Furthermore, treatment with TMP significantly improved the dopamine deficits and attenuated the upregulation of striatal basal glutamatergic strength in the striatum of mice injected with MPTP. These results indicated that TMP might serve as a novel approach for the treatment of patients with PD.


Assuntos
Citocinas/metabolismo , Dopamina/metabolismo , Transtornos Parkinsonianos , Pirazinas/uso terapêutico , Vasodilatadores/uso terapêutico , Análise de Variância , Animais , Benzofuranos , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Corpo Estriado/patologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Técnicas In Vitro , Masculino , Camundongos , Neurônios/efeitos dos fármacos , Transtornos Parkinsonianos/tratamento farmacológico , Transtornos Parkinsonianos/patologia , Transtornos Parkinsonianos/fisiopatologia , Técnicas de Patch-Clamp , Equilíbrio Postural/efeitos dos fármacos , Desempenho Psicomotor/efeitos dos fármacos , Quinolinas
13.
World J Surg ; 38(10): 2645-51, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24806624

RESUMO

BACKGROUND: Complete video-assisted thoracoscopic surgery (c-VATS) for left upper lobectomy is difficult due to the branching pattern of the left pulmonary artery. OBJECTIVE: Our purpose was to report outcomes of a modified technique of c-VATS left upper lobectomy. METHODS: We retrospectively compared the outcomes of 83 patients with stage I/II non-small-cell lung cancer (NSCLC) who received left upper lobectomy between 2008 and 2011; 32 underwent conventional c-VATS and 50 received modified c-VATS. In the modified procedure, the order in which hilum of lung was treated was from the lingular segmental artery to the superior pulmonary vein to the bronchus, and then finally the pulmonary artery. RESULTS: The mean patient age was 63.6 ± 8.4 years, and no differences were observed in age, gender, and largest tumor diameter between the two groups. No conversion occurred in either group. The surgical time for modified c-VATS was significantly shorter than that for conventional c-VATS (210 vs. 270 min, p < 0.001). Drainage time after surgery and length of hospitalization for the modified c-VATS group were significantly less than those for the conventional group (drainage 3 vs. 4 days, respectively, p = 0.041; length of hospitalization 7 versus 12 days, respectively; p < 0.001). Surgical margins were clear in all cases. Four (8.0 %) complications occurred in the modified procedure group compared with ten (31.3 %) in the conventional group (p = 0.015). CONCLUSION: This new technique offers shorter surgical and postoperative drainage time, shorter hospital stays, and fewer complications than conventional c-VATS upper left lobectomy.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/cirurgia , Neoplasias Pulmonares/cirurgia , Pneumonectomia/métodos , Artéria Pulmonar/cirurgia , Cirurgia Torácica Vídeoassistida/métodos , Idoso , Carcinoma Pulmonar de Células não Pequenas/patologia , Drenagem , Feminino , Humanos , Tempo de Internação , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Neoplasia Residual , Duração da Cirurgia , Estudos Retrospectivos
14.
Basic Clin Pharmacol Toxicol ; 112(1): 19-24, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22998524

RESUMO

Glutamate transporter-1 (GLT-1) plays a dual role in glutamate transportation: both normally devotion to the clearance of glutamate and during some pathological conditions extruding glutamate to the extracellular space. Therefore, it is uncertain whether increased expression of GLT-1 will actually be helpful against glutamate excitotoxicity. In this study, GLT-1 up-regulation was induced by ceftriaxone, and L-glutamate was added to induce glutamate toxicity in primary cultured rat cortical cells. The results showed that up-regulated GLT-1 induced by 1 µM ceftriaxone for 2 days markedly increased cell viability, decreased apoptotic cell death and alleviated ultrastructural damage induced by 50 µM glutamate 15 min. as well as promoted L-[(3) H]-glutamate uptake in cultured cells. GLT-1 up-regulation had no effect on the intracellular free calcium concentration ([Ca(2+) ](i) ) in the resting situation, while relieved intracellular calcium overloading by reducing the elevation and promoting the recovery of [Ca(2+) ](i) following stimulation of 50 µM glutamate for 2 min. Applying 100 µM dihydrokainic acid (GLT-1 antagonist) 30 sec. before glutamate eliminated the above effect of GLT-1 up-regulation on [Ca(2+) ](i) . In conclusion, GLT-1 up-regulation induced by ceftriaxone plays a positive glutamate transporting role against glutamate toxicity in primary cultured rat cortical cells.


Assuntos
Cálcio/metabolismo , Córtex Cerebral/efeitos dos fármacos , Transportador 2 de Aminoácido Excitatório/fisiologia , Ácido Glutâmico/toxicidade , Animais , Ceftriaxona/farmacologia , Células Cultivadas , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Ácido Glutâmico/metabolismo , Ratos , Ratos Wistar , Regulação para Cima
15.
Biol Trace Elem Res ; 145(2): 225-32, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21898106

RESUMO

Greenhouse experiments were conducted to study the permissible value of vanadium (V) based on the growth and physiological responses of green Chinese cabbage (Brassica chinensis L.), and effects of V on microbial biomass carbon (MBC) and enzyme activities in allitic udic ferrisols were also studied. The results showed that biomass of cabbage grown on soil treated with 133 mg V kg(-1) significantly decreased by 25.1% compared with the control (P < 0.05). Vanadium concentrations in leaves and roots increased with increasing soil V concentration. Contents of vitamin C (Vc) increased by 10.3%, while that of soluble sugar in leaves significantly decreased by 54.0% when soil V concentration was 133 mg kg(-1), respectively. The uptake of essential nutrient elements by cabbage was disturbed when soil V concentration exceeded 253 mg kg(-1). Soil MBC was significantly stimulated by 15.5%, while dehydrogenase activity significantly decreased by 62.8% and urease activity slightly changed at treatment of 133 mg V kg(-1) as compared with the control, respectively. Therefore, the permissible value of V in allitic udic ferrisols is proposed as 130 mg kg(-1).


Assuntos
Brassica/metabolismo , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Microbiologia do Solo , Solo , Vanádio/metabolismo , Vanádio/farmacologia , Brassica/crescimento & desenvolvimento , Brassica/microbiologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/microbiologia , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/microbiologia
16.
Neurosci Bull ; 25(3): 139-52, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19448688

RESUMO

The mechanisms of brain ischemic insult include glutamate excitoxicity, calcium toxicity, free radicals, nitric oxide, inflammatory reactions, as well as dysfunctions of endoplasmic reticulum and mitochondrion. These injury cascades are interconnected in complex ways, thus it is hard to compare their pathogenic importances in ischemia models. And the research in cellular and molecular pathways has spurred the studies in potential neuroprotections mainly in pharmacological fields, such as anti-excitotoxic treatment, calcium-channel antagonism, approaches for inhibition of oxidation, inflammation and apoptosis, etc. Besides, other protective interventions including thrombolysis, arteriogenesis, regeneration therapy, and ischemia preconditioning or postconditioning, are also under investigations. Despite the present difficulties, we are quite optimistic towards future clinical applications of neuroprotective agents, by optimizing experimental approaches and clinical trials.


Assuntos
Isquemia Encefálica/prevenção & controle , Isquemia Encefálica/fisiopatologia , Animais , Isquemia Encefálica/metabolismo , Humanos , Modelos Biológicos , Fármacos Neuroprotetores/farmacologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia
17.
J Environ Sci (China) ; 17(2): 332-4, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16295916

RESUMO

Through a batch experiment, the mobility and speciation of heavy metals (Cd, Cu, Zn) in two acidic forest soils from Hunan Province were studied. The results showed that the release and potential active speciation of Cd, Cu, and Zn in the tested contaminated red soil (CRS) and yellow red soil (CYRS) increased significantly with pH decreasing and ion concentrations increasing of simulated acid rain, and these effects were mainly decided by the pH value of simulated acid rain. Cd had the highest potential risk on the environment compared with Cu and Zn. Cd existed mainly in exchangeable form in residual CRS and CYRS, Cu in organically bound and Mn-oxide occluded forms, and Zn in mineral forms due to the high background values.


Assuntos
Chuva Ácida/análise , Meio Ambiente , Metais Pesados/química , Solo/análise , China , Concentração de Íons de Hidrogênio , Espectrofotometria Atômica
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