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1.
Eur J Med Chem ; 198: 112354, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32387837

RESUMO

Inhibition of transforming growth factor ß (TGF-ß) type 1 receptor (ALK5) provides a feasible approach for the treatment of fibrotic diseases and malignant tumors. In this study, we designed and synthesized a new series of 4-(pyridin-4-oxy)-3-(3,3-difluorocyclobutyl)-pyrazole derivatives, and evaluated biologically as TGF-ß type 1 receptor inhibitors. The most potent compound 15r inhibited the ALK5 enzyme and NIH3T3 cell viability with IC50 values of 44 and 42.5 nM, respectively. Compound 15r also displayed better oral plasma exposure and excellent bioavailability than LY-3200882, and in vivo inhibited 65.7% of the tumor growth in a CT26 xenograft mouse model.

2.
J Cardiothorac Surg ; 15(1): 49, 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32293486

RESUMO

BACKGROUND: Aortic intramural hematoma (IMH) is a subset of acute aortic syndrome, and its prognosis may differ between races. This study aimed to study the prognosis of Chinese type B IMH patients and to find out risk factors. METHODS: A total of 71 type B IMH patients with or without penetrating atherosclerosis ulcer (PAU) administrated in our center between September 2013 and October 2017 were retrospectively studied. Both clinical and imaging data were collected and analyzed. The primary end point was aorta-related death, and the secondary end point was progression, which was defined as enlargement of aorta, increased aortic wall thickness, and aortic dissection or aneurysm formation. Kaplan-Meier survival analysis and Cox regression analysis were used for prognostic analysis. RESULTS: Among these 71 patients, 21 had simple type B IMH, when 50 had type B IMH in association with PAU. Twenty-five patients received optimal medical therapy (OMT) alone, while 46 patients received surgery and OMT. The mean follow-up time was 27.5 ± 13.5 months. For type B IMH patients, association with PAU indicated poor prognosis and required more intensive management (HR = 16.68, 1.96~141.87), while maximum aortic diameter (MAD) was an independent risk factor (HR = 1.096, 1.016~1.182). For patients with PAU-IMH, MAD was an independent risk factor (HR = 1.04, 1.021~1.194), while surgical treatment was independent protective factor (HR = 0.172, 0.042~0.696). CONCLUSION: Association with PAU and MAD were independent risk factors for type B IMH patients. Surgery may improve the outcomes for type B IMH in association with PAU.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32129738

RESUMO

A lactic acid bacterial strain, HBUAS57009T, isolated from traditionally fermented food (Zha-Chili) in China, was characterized to clarify its taxonomic status using a polyphasic approach. Strain HBUAS57009T was phylogenetically closely related to Lactobacillus koreensis DCY50T, Lactobacillus fujinensis 218-6T, Lactobacillus mulengensis 112-3T, Lactobacillus cerevisiae TUM BP 140423000-2250T, Lactobacillus tongjiangensis 218-10T and Lactobacillus yonginensis THK-V8T with sequence similarities of 98.6-99.3 %. The genome-to-genome distance and average nucleotide identity values between the genomes of strain HBUAS57009T and type strains of closely related Lactobacillus species were less than 32.0 and 86.0 %, respectively; this is below the threshold for species boundaries. The major cellular fatty acids (>10 %) were C16 : 0, C18 : 1 ω9c and iso-C19 : 0. The G+C content of the genomic DNA of strain HBUAS57009T was 47.8 mol%. Examination of the functional categories of the genome revealed that strain HBUAS57009T could perform both homolactic and heterolactic fermentation processes to produce lactic acid via complete glycolysis and the pentose phosphate pathway. The putative biosynthesis pathway of butane-2,3-diol and acetoin, two important flavour compounds in the food industry, were identified using kegg mapper analysis. Based on its genotypic and phenotypic features, strain HBUAS57009T (=GDMCC 1.1664T=KACC 21424T) is designated as the type strain of a novel species, for which the name Lactobacillus enshiensis sp. nov. is proposed.

4.
Curr Microbiol ; 77(5): 882-889, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31950235

RESUMO

Rice wine, a traditional fermented alcoholic beverage in China, is produced with grains such as rice, which are fermented with saccharifying starter-koji. Its flavor quality is closely associated to the starter culture-koji, which is made by mixing botanical materials with high-class glutinous rice in certain ecological context. However, there are few reports on the microbial community structure of rice wine koji. In this paper, bacterial community structures of rice wine koji were analyzed using 16S rRNA gene sequencing based on Illumina MiSeq high-throughput technology in 20 samples collected from Xiaogan area, Hubei province and Dazhu area, Sichuan province (10 from each area). We found rice wine koji flora mainly consisted of Weissella, Lactobacillus, Lactococcus, Bacillus, Enterococcus, and Cronobacter, with relative abundances of 29.49%, 10.93%, 8.85%, 4.75%, 1.16% and 1.15%, respectively, as well as an accumulative average relative abundance of 58.71%. They all belonged to Firmicutes and Proteobacteria-the two known dominant genus. Genus-level PCA (Principal component analysis) and OTU-level PCoA (Principal coordinates analysis) based on unweighted UniFrac distances showed that the bacterial community structure differed significantly between the samples from the 2 areas. 7 OTUs were detected in all samples, accounting for 4.4% of the total qualified assembly. Among the 7 OTUs, 3 OTUs were identified as Enterococcus, 2 OTUs were identified as Cronobacter, 1 OTU was identified as Bacillus and 1 OTU was identified as Alkaliphilus. Fifty-eight lactic acid bacteria (LAB) strains were isolated from the 20 koji samples with traditional microbial methods. Among them, Enterococcus faecium and Pediococcus pentosaceus were the dominant LAB isolates, with relative abundances of 51.72% and 31.03%. Despite the differences, a large number of shared bacteria were detected in samples from the two areas.

5.
World Neurosurg ; 134: e12-e15, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31465849

RESUMO

OBJECTIVE: We explored the risk factors for the occurrence of delayed facial paralysis (DFP) after microvascular decompression (MVD) for hemifacial spasm (HFS). METHODS: A retrospective study was conducted of 636 patients who had undergone MVD for HFS by the same neurosurgery department of China-Japan Friendship Hospital from January 2006 to May 2016. Of the 636 patients, 50 (7.9%) had presented with DFP, which had developed from 2 to 60 days postoperatively (average, 12.9 ± 10.0005 days). All 50 patients with DFP had recovered completely within 10-300 days (average, 88.7 ± 61.389 days) after the onset of DFP. We randomly selected 100 patients from the 586 patients without DFP as the control group. Univariate and multivariate logistic analyses were used to analyze the risk factors involved in the occurrence of DFP. RESULTS: Univariate analysis showed that the disease course was the only factor associated with the development of DFP (P = 0.003). Furthermore, on multivariate logistic analysis, the course of HFS was the only risk factor associated with the development of DFP (P = 0.01). Additionally, the Spearman test revealed a positive correlation between the onset of DFP and the duration of the DFP symptoms (rs = 0.682; P < 0.001). CONCLUSION: Although DFP frequently occurred after MVD, it can recover spontaneously. The longer the course of HFS, the more frequently DFP will occur after MVD. The earlier that DFP develops, the shorter will be the time to recovery.

6.
J Hazard Mater ; 384: 121486, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31668756

RESUMO

Metal-free mesoporous graphitic frameworks with built-in nanotubes (CPGs) were synthesized via facile co-pyrolysis of cyclodextrin and a cobalt salt with subsequent acid pickling to remove the embedded metal species. Due to the high graphitic degree and built-in few-layer nanotubes, the as-synthesized carbonaceous materials possess a higher catalytic ozonation activity than that of the state-of-the-art carbon nanotubes (CNTs) and LaMnO3 perovskite catalysts for the destruction of different aqueous contaminants. For recalcitrant oxalic acid removal, 50 mg L-1 oxalic acid was completely degraded in 20 min. Compared with other nanocarbons, the as-synthesized materials also demonstrated robust structural stability and reusability. The electron paramagnetic resonance (EPR) and selective radical quenching tests revealed that the destruction of the aqueous organics predominantly relied on surface-adsorbed complexes (O*ad and O2*) from activated ozone molecules. Owing to the occurrence of this surface oxidation pathway, the compatibility of the CPGs/O3 system was significantly enhanced for treatment of real wastewater, where the inorganic anions and organic natural organic matter would inhibit radical oxidation as radical scavengers. Furthermore, by employing organics with different ionization potentials (IPs) as the target pollutants, the CPGs/O3 system was discovered to obtain a high oxidation potential.

7.
Emerg Microbes Infect ; 8(1): 1229-1239, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31448697

RESUMO

Lysine acetylation and succinylation are both prevalent protein post-translational modifications (PTMs) in bacteria species, whereas the effect of the cross-talk between both PTMs on bacterial biological function remains largely unknown. Our previously study found lysine succinylated sites on proteins play important role on metabolic pathways in fish pathogenic Aeromonas hydrophila. A total of 3189 lysine-acetylation sites were further identified on 1013 proteins of this pathogen using LC-MS/MS in this study. Functional examination of these PTMs peptides showed associations with basal biological processes, especially metabolic pathways. Additionally, when comparing the obtained lysine acetylome to a previously obtained lysine succinylome, 1198 sites in a total of 547 proteins were found to be in common and associated with various metabolic pathways. As the autoinducer-2 (AI-2) synthase involved in quorum sensing of bacteria, the site-directed mutagenesis of LuxS at the K165 site was performed and revealed that the cross-talk between lysine acetylation and succinylation exerts an inverse influence on bacterial quorum sensing and on LuxS enzymatic activity. In summary, this study provides an in-depth A. hydrophila lysine acetylome profile and for the first time reveals the role of cross-talk between lysine acetylation and succinylation, and its potential impact on bacterial physiological functions.


Assuntos
Aeromonas hydrophila/metabolismo , Proteínas de Bactérias/metabolismo , Liases de Carbono-Enxofre/metabolismo , Lisina/metabolismo , Processamento de Proteína Pós-Traducional , Acetatos/análise , Acetilação , Aeromonas hydrophila/química , Aeromonas hydrophila/fisiologia , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Liases de Carbono-Enxofre/química , Liases de Carbono-Enxofre/genética , Cromatografia Líquida , Análise Mutacional de DNA , Lisina/química , Mutagênese Sítio-Dirigida , Percepção de Quorum , Ácido Succínico/análise , Espectrometria de Massas em Tandem
8.
J Am Chem Soc ; 141(30): 12005-12010, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31276405

RESUMO

The peroxone reaction between O3 and H2O2 has been deemed a promising technology to resolve the increasingly serious water pollution problem by virtue of the generation of superactive hydroxyl radicals (•OH), but it suffers greatly from an extremely limited reaction rate constant under acidic conditions (ca. less than 0.1 M-1 s-1 at pH 3). This article describes a heterogeneous catalyst composed of single Mn atoms anchored on graphitic carbon nitride, which effectively overcomes such a drawback by altering the reaction pathway and thus dramatically promotes •OH generation in acid solution. Combined experimental and theoretical studies demonstrate Mn-N4 as the catalytically active sites. A distinctive catalytic pathway involving HO2• formation by the activation of H2O2 is found, which gets rid of the restriction of HO2- as the essential initiator in the conventional peroxone reaction. This work offers a new pathway of using a low-cost and easily accessible single-atom catalyst (SAC) and could inspire more catalytic oxidation strategies.

9.
Int J Syst Evol Microbiol ; 69(8): 2196-2201, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30900972

RESUMO

Strain HBUAS52074T is a Gram-positive staining, aerobic bacterium that was isolated from Zha-Chili, a traditional fermented food made in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HBUAS52074T is a member of the genus Lactobacillus and closely related to Lactobacillus nantensis DSM 16982T (98.9 %), Lactobacillus heilongjiangensis DSM 28069T (98.8 %), Lactobacillus formosensis NBRC 1095009T (98.6 %), Lactobacillus futsaii JCM 17355T (98.5 %), Lactobacillus farciminis KCTC 3681T (98.5 %), Lactobacillus musae NBRC 112868T (98.5 %) and Lactobacillus crustorum LMG 23699T (98.4 %). The DNA G+C content is 36.3 mol%. The major cellular fatty acids are C16 : 0 (28.2 %), C18 : 1ω9c (30.5 %) and summed feature 7 (C19 : 1ω6c, and/or C19 : 1ω7c; 14.9 %). Average nucleotide identity and DNA-DNA hybridization (GGDC) values based on genomic comparisons between HBUAS52074T and related type species showed that the bacterium was significantly different from its closest relatives. Using polyphasic taxonomic analysis, we have shown that strain HBUAS52074T is a new species in the genus Lactobacillus, for which we propose the name Lactobacilluszhachilii sp. nov. The type strain is HBUAS52074T (=GDMCC 1.1417T=KCTC 21106T).


Assuntos
/microbiologia , Lactobacillus/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Ácido Láctico , Lactobacillus/isolamento & purificação , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
10.
ACS Appl Mater Interfaces ; 11(10): 10399-10408, 2019 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-30775907

RESUMO

Interfacial charge transfer and structural proximity effects are the two essential routes to trigger and tune numerous functionalities of perovskite oxide heterostructures. However, the cooperation and competition of these two interfacial effects in one epitaxial system have not been fully understood. Herein, we fabricate a series of La0.67Ca0.33MnO3/CaRuO3 superlattices and introduce various chemical doping in the nonmagnetic CaRuO3 interlayers. We found that Ti, Sr, and La doping in the CaRuO3 layer can effectively tune the interfacial charge transfer and octahedral rotation, thus modulating the ferromagnetism of the superlattices. Specifically, the B-site Ti doping depletes the Ru 4d band and suppresses the interfacial charge transfer, leading to a decay of ferromagnetic Curie temperature ( TC). In contrast, the A-site Sr doping maintains a sizable charge transfer and meanwhile suppresses the octahedral rotation, which facilitates ferromagnetism and significantly enhances the TC up to 291 K. The La doping turns out to localize the itinerant electrons in the CaRuO3 layer, which suppresses both the interfacial charge transfer and ferromagnetism. The observed intriguing interfacial engineering of magnetism would pave a new way to understand the collective effects of interfacial charge transfer and structural proximity on the physical properties of oxide heterostructures.

11.
Int J Syst Evol Microbiol ; 69(5): 1313-1319, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801241

RESUMO

Strain HBUAS61001T was isolated from the pickling sauce used to make a traditional fermented food product, datoucai, in China. The strain belonged to the genus Vibrio, but was placed in a clade separate from any known Vibrio species based on the 16S rRNA gene and MLSA results. The genome consisted of two chromosomes: chromosome I was 2 901 449 bp long with a G+C content of 45.4 mol%; and chromosome II was 1 107 930 bp long with a G+C content of 45.5 mol%. The most abundant fatty acids were C16 : 0 (28.1 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 29.4 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c, 10.1 %). The isoprenoid quinones detected were Q7 and Q8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain HBUAS61001T could grow in the presence of up to 17 % NaCl. The calculated average nucleotide identity and in silico DNA-DNA hybridization (GGDC) values of the strain against the closest related type strains were all lower than 95 and 70 %, respectively. Putative genes in the genome associated with survival under high salinity stress were identified. Based on whole genome sequence analysis and phenotypic characteristics, strain HBUAS61001T is a new species in the genus Vibrio, and the name Vibrio zhugei (=GDMCC 1.1416T=KCTC 62784T) is proposed.


Assuntos
/microbiologia , Microbiologia de Alimentos , Filogenia , Vibrio/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
12.
Int J Syst Evol Microbiol ; 69(1): 139-145, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30614783

RESUMO

In this study, we describe a new genus and species of yeast with high-salt tolerance. The strain was isolated from the pickling sauce used to make Datoucai, a traditional fermented food made from Brassica juncea in Xiangyang, China. Phylogenetic analysis of sequences from the D1/D2 region of the LSU rRNA gene and from the ITS region demonstrated that the strain, reference HBUAS51001T, was most closely related to members of the genera Occultifur and Cystobasidium. However, the greatest similarities between the D1/D2 and ITS nucleotide sequences of strain HBUAS51001T and the most closely related type strains from Occultifur and Cystobasidium were only 91 and 92 %, respectively. This suggests that strain HBUAS51001T does not belong to any currently described species. Strain HBUAS51001T grew readily on media in which xylose was the sole carbon source. The major ubiquinone was Q9. The genome of strain HBUAS51001T was 42.42 Mb with a G+C content of 53.93 mol%. Three candidate genes associated with xylose metabolism were identified. On the basis of genotypic and phenotypic data, strain HBUAS51001T can be considered as both a new species and a new genus, for which the name Halobasidium xiangyangense gen. nov., sp. nov. is proposed. The type strain is HBUAS51001T (=KCTC27810T=GDMCC 2.231T=CCTCC AY 2018002T).


Assuntos
Basidiomycota/classificação , Filogenia , Xilose/metabolismo , Composição de Bases , Basidiomycota/genética , Basidiomycota/isolamento & purificação , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
13.
Chemosphere ; 222: 38-45, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30690399

RESUMO

Photocatalytic ozonation is an attractive advanced oxidation process for wastewater treatment, but highly active catalysts with strong response to visible light are urgently needed to push forward its practical application. In this study, a hierarchical biomimetic monoclinic bismuth vanadate (BiVO4) with leaves morphology was synthesized by a hydrothermal method, and employed as catalyst for oxalic acid and penicillin degradation in photocatalytic ozonation. The results show that the organics degradation was more efficient using leaves shaped BiVO4 as catalyst than the bulk shaped one in photocatalytic ozonation and the synergy index is ranged from 2.8 to 3.3, indicating a superior positive synergistic effect between photocatalysis and ozonation. The higher activity of the leaves shaped BiVO4 was probably attributed to the distinctive biomimetic morphology and preferable band structure with more negative CB potential. Mechanism studies suggested that the main reactive species were h+ and OH for the degradation of persistent oxalic acid in photocatalytic ozonation. In addition, the effect of ozone concentration and inorganic ions and reusability of the material were also intensively investigated.


Assuntos
Biomimética/métodos , Bismuto/química , Luz , Ozônio , Vanadatos/química , Águas Residuárias , Catálise , Ácido Oxálico/química , Oxirredução , Penicilinas/química , Fotólise , Vanadatos/síntese química , Poluentes Químicos da Água
14.
Mol Cell Proteomics ; 18(2): 200-215, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30352804

RESUMO

Protein modification by lysine succinylation is a newly identified post-translational modification (PTM) of lysine residues and plays an important role in diverse physiological functions, although their associated biological characteristics are still largely unknown. Here, we investigated the effects of lysine succinylation on the physiological regulation within a well-known fish pathogen, Aeromonas hydrophila A high affinity purification method was used to enrich peptides with lysine succinylation in A. hydrophila ATCC 7966, and a total of 2,174 lysine succinylation sites were identified on 666 proteins using LC-MS/MS. Gene ontology analysis indicated that these succinylated proteins are involved in diverse metabolic pathways and biological processes, including translation, protein export, and central metabolic pathways. The modifications of several selected candidates were further validated by Western blotting. Using site-directed mutagenesis, we observed that the succinylation of lysines on S-ribosylhomocysteine lyase (LuxS) at the K23 and K30 sites positively regulate the production of the quorum sensing autoinducer AI-2, and that these PTMs ultimately alter its competitiveness with another pathogen, Vibrio alginolyticus Moreover, subsequent metabolomic analyses indicated that K30 succinylation on LuxS may suppress the activated methyl cycle (AMC) and that both the K23 and K30 sites are involved in amino acid metabolism. Taken together, the results from this study provide significant insights into the functions of lysine succinylation and its critical roles on LuxS in regulating the cellular physiology of A. hydrophila.


Assuntos
Aeromonas hydrophila/fisiologia , Proteínas de Bactérias/metabolismo , Liases de Carbono-Enxofre/metabolismo , Lisina/química , Metabolômica/métodos , Percepção de Quorum , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Liases de Carbono-Enxofre/química , Liases de Carbono-Enxofre/genética , Cromatografia Líquida , Ontologia Genética , Homosserina/análogos & derivados , Homosserina/metabolismo , Lactonas/metabolismo , Lisina/genética , Metaboloma , Mutagênese Sítio-Dirigida , Processamento de Proteína Pós-Traducional , Espectrometria de Massas em Tandem
15.
Artigo em Inglês | MEDLINE | ID: mdl-30460208

RESUMO

Outer membrane proteins (OMPs) play essential roles in antibiotic resistance, particularly in Gram-negative bacteria; however, they still have many unidentified functions regarding their behavior in response to antibiotic stress. In the current work, quantitative tandem mass tag labeling-based mass spectrometry was used to compare the outer membrane related proteins between an oxytetracycline-resistant (OXY-R) and its original control stain (OXY-O) in Aeromonas hydrophila. Consequently, a total of 261 commonly altered proteins in two biological repeats were identified including 29 proteins that increased and 28 that decreased. Gene ontology analysis showed that the expression of transport proteins was significantly reduced, and translation-related proteins were downregulated in the OXY-R strain. After using western blotting to validate selected altered proteins, eight OMP-related genes were knocked out and their roles in antibiotic resistance were further evaluated. The survival assays showed that some mutants such as ΔAHA_4281, ΔAHA_2766, ΔAHA_2282, ΔAHA_1181, and ΔAHA_1280 affected the susceptibility of A. hydrophila to antimicrobials. Moreover, the minimum inhibitory concentration assay showed that these candidate mutants also respond differently to other types of antibiotics. Our results reveal several novel outer membrane related proteins of A. hydrophila that play important roles in antibiotic resistance, and as such, may be helpful for screening studies to identify novel drug targets.


Assuntos
Aeromonas hydrophila/metabolismo , Proteínas da Membrana Bacteriana Externa/metabolismo , Resistência Microbiana a Medicamentos/fisiologia , Proteômica/métodos , Aeromonas hydrophila/efeitos dos fármacos , Aeromonas hydrophila/genética , Proteínas da Membrana Bacteriana Externa/efeitos dos fármacos , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/genética , Western Blotting , Regulação para Baixo , Resistência Microbiana a Medicamentos/efeitos dos fármacos , Resistência Microbiana a Medicamentos/genética , Técnicas de Inativação de Genes , Redes e Vias Metabólicas , Testes de Sensibilidade Microbiana , Oxitetraciclina/farmacologia , Proteínas Recombinantes , Espectrometria de Massas em Tandem
16.
J Hazard Mater ; 360: 481-489, 2018 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-30144767

RESUMO

Photocatalytic ozonation has great potential in wastewater treatment. However, the role of ozone and the contribution of photogenerated hole in this process have not been fully understood. Here three WO3 materials are synthesized and used as model catalysts in visible-light photocatalytic ozonation for the mineralization of pharmaceutical pollutants. A dual role of ozone in this process has been confirmed: (i) direct oxidation of the pollutant till formation of refractory intermediates, (ii) efficient trapping of photoelectron that cannot be captured by O2. The latter is crucial because it not only induces the O3--mediated pathway for hydroxyl radical (OH) formation but also separates the hole which has proven to be capable of oxidizing water into OH. Evidenced by photoluminescence results, the intrinsic charge separation ability of WO3 in photocatalytic ozonation is no more as important as that in photocatalysis with O2. Finally, this process is more applicable under acidic condition. This work contributes to a better understanding of the significance of ozone in WO3 photocatalytic ozonation and provides us an insight into the mechanism of photocatalytic ozonation.


Assuntos
Oxidantes/química , Óxidos/química , Ozônio/química , Preparações Farmacêuticas/química , Tungstênio/química , Poluentes Químicos da Água/química , Catálise , Luz , Óxidos/efeitos da radiação , Fotólise , Tungstênio/efeitos da radiação , Purificação da Água/métodos
17.
Fish Shellfish Immunol ; 82: 50-59, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30086377

RESUMO

Iron-related proteins play important roles in iron homeostasis, and they may be potential vaccine candidates against pathogenic Aeromonas hydrophila. In addition, the encapsulation of antigens in single-walled carbon nanotubes (SWCNTs) has recently been shown to effectively stimulate the host immune response. To investigate the immune response of zebrafish to iron-related proteins and SWCNT-encapsulated proteins, we overexpressed and purified four iron-related recombinant proteins (P55870, A0KGK5, A0KPP0, and A0KIY3) from A. hydrophila. We then vaccinated zebrafish with these proteins and their SWCNT-encapsulated counterparts via both intraperitoneal injection and bath immunization. The target proteins evoked an immune response in zebrafish after intraperitoneal injection, and SWCNT-encapsulation significantly increased the immune response after bath immunization. When challenged with virulent A. hydrophila, zebrafish administered 5 µg intraperitoneal injections of SWCNT-P55870, A0KGK5, A0KPP0, or A0KIY3 had remarkably high relative percent survivals (RPSs) (50%, 55.6%, 66.7%, and 94.44% respectively). The RPSs of zebrafish vaccinated via immunization bath with 40 mg/L SWCNT-encapsulated counterparts were also high (52.94%, 55.56%, 61.11%, and 86.11%, respectively). These results indicated that zebrafish vaccinated with P55870, A0KGK5, SWCNT-P55870, and SWCNT-A0KGK5 were partially protected, while A0KPP0 and A0KIY3 were promising vaccine candidates against pathogenic A. hydrophila infection.


Assuntos
Proteínas de Bactérias/farmacologia , Doenças dos Peixes/imunologia , Infecções por Bactérias Gram-Negativas/veterinária , Imunidade Inata , Nanotubos de Carbono/análise , Peixe-Zebra , Aeromonas hydrophila/fisiologia , Animais , Infecções por Bactérias Gram-Negativas/imunologia , Injeções Intraperitoneais/veterinária , Ferro , Distribuição Aleatória , Proteínas Recombinantes/farmacologia
18.
Artigo em Inglês | MEDLINE | ID: mdl-29552083

RESUMO

Danhong injection (DHI) has been widely used in China for cardiocerebrovascular diseases treatments. And in this study, we demonstrated the therapeutic effect of DHI on experimental diabetic neuropathy for the first time. Methods. Streptozotocin- (STZ-) induced SD rats were used. In experiment 1, 4-week treatment with DHI or saline started 4 weeks after STZ injection; mechanical allodynia was measured before and every 2 weeks after STZ injection. In experiment 2, chronic intrathecal infusion of U0126 was conducted during the 8th week of diabetes. Phosphorylated and total ERK1/2 in spinal cord were analyzed by western blot. BDNF level in sciatic nerve was evaluated by ELISA. Results. DHI treatment significantly alleviated mechanical allodynia at the end of the study and downregulated the expression of phosphorylated ERK1/2 in spinal cord. In addition, DHI treatment also elevated brain-derived neurotrophic factor (BDNF) level in sciatic nerve of DPN rat. In experiment 2, inhibition of ERK1/2 activation was confirmed to result in the alleviation of mechanical allodynia. Conclusions. We demonstrated that DHI was able to alleviate mechanical allodynia in diabetic neuropathy rat through inhibiting the activation of ERK1/2. The reduction of BDNF content in sciatic nerve was also partially reversed by DHI treatment.

19.
Chemosphere ; 201: 206-213, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29524821

RESUMO

Three kinds of graphitic carbon nitride materials (bulk, porous and nanosheet g-C3N4) were composited with a multiwall carbon nanotube (MWCNT) by a hydrothermal method, and the obtained b-C3N4/CNT, p-C3N4/CNT and n-C3N4/CNT materials were used in the electrodes for electro-peroxone process. It was found that the n-C3N4/CNT composite exhibited the highest efficiency in oxalate degradation, though it performed the worst in the oxygen-reduction reaction for H2O2 production. The n-C3N4/CNT composite exhibited higher activity than CNT and other composites in catalytic ozonation experiments, due to the higher pyrrolic-N content modified on the CNT surface and higher surface area. It also has higher electron transfer ability, which benefited to the electro-reduction of both O2 and O3. The result confirmed that catalytic ozonation process was an important means to enhance the degradation efficiency in the electro-peroxone process, besides peroxone process and O3-electrolysis.


Assuntos
Eletrodos , Eletrólise , Grafite/química , Nanotubos de Carbono/química , Nitrilos/química , Ozônio/química , Peróxido de Hidrogênio , Oxirredução , Poluentes Químicos da Água
20.
Plast Reconstr Surg ; 141(3): 397e-403e, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29481409

RESUMO

BACKGROUND: Peripheral nerve decompression surgery has been reported to be effective for pain reduction in patients with painful diabetic peripheral neuropathy. The aim of this study was to characterize which patients may have more pain relief benefits in the lower limbs after nerve decompression surgery. METHODS: A retrospective study was conducted. Pain levels were measured with the Numerical Rating Scale. Treatment effects were classified by either substantial relief (at least 50 percent reduction in Numerical Rating Scale score compared with preoperative Numerical Rating Scale score) or nonsubstantial relief (<50 percent reduction or worse in Numerical Rating Scale score) at 12 months based on established criteria. Sex, age, body mass index, duration of diabetes mellitus, duration of diabetic peripheral neuropathy pain, preoperative Numerical Rating Scale score, and two-point discrimination were evaluated using univariate and logistic regression analysis. RESULTS: The mean preoperative Numerical Rating Scale score (8.65 ± 1.29) decreased significantly 6 days (3.56 ± 2.22; p < 0.01), 6 months (3.03 ± 2.11; p < 0.01), and 12 months (3.44 ± 2.36; p < 0.01) after surgery; 64.7 percent of patients had substantial pain relief at 12 months. According to univariate and logistic regression analysis, better two-point discrimination was associated with substantial pain relief (OR, 3.700; p = 0.046, logistic regression analysis). CONCLUSIONS: Nerve decompression surgery was able to alleviate pain in patients with painful diabetic peripheral neuropathy. Two-point discrimination may be a predictive factor for the prognosis of painful diabetic peripheral neuropathy after nerve decompression surgery. CLINICAL QUESTION/LEVEL OF EVIDENCE: Risk, III.


Assuntos
Neuropatias Diabéticas/diagnóstico , Neuropatias Diabéticas/cirurgia , Dor Pós-Operatória/diagnóstico , Percepção do Tato/fisiologia , Adulto , Idoso , Neuropatias Diabéticas/fisiopatologia , Feminino , Seguimentos , Humanos , Modelos Logísticos , Extremidade Inferior/cirurgia , Masculino , Pessoa de Meia-Idade , Medição da Dor , Nervos Periféricos/cirurgia , Complicações Pós-Operatórias , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos
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