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1.
Artigo em Inglês | MEDLINE | ID: mdl-30479220

RESUMO

OBJECTIVE: To explore the cytotoxic and apoptotic activity of the pierisin-6 protein in HPV HeLa and HepG2 cell lines. METHODS: In this study, isolation and purification of cytotoxic prierisin-6 from the larvae of Pieris napi by affinity column chromatography techniques.Characterization of full-length mRNA of pierisin-6 gene was performed using 3'/5' RACE PCR. The quantitative RT-PCR used to study the developmental stage-specific expression of pierisin-6 mRNA. The most effective concentration of pierisin-6 protein was determined by measuring cell proliferation. Apoptosis was assessed using AO/Et-Br, Propidium Iodide and Rhodamine 123 assays, whereas protein levels of caspase 3, cytochrome C were evaluated by ELISA method. Pierisin-6 induced cell cycle arrest was determined using Propidium iodide by FACS. RESULTS: In this study, Pierisin-6, a novel apoptotic protein was found to have cytotoxicity against HeLa at IC50 of 2.02 ng/ml and HepG2 human cancer cell lines. Flow cytometry analysis confirms an increased percentage of apoptotic cells in sub G1 phase and cell cycle arrest at S phase. Alteration in the transmembrane potential of mitochondria, Cytochrome c released from mitochondrial membrane, and caspase substrate assay demonstrated the cleavage of Ac-DEVD-pNA signifying the activation of Caspase-3. These findings suggested that Pierisin-6 significantly induce apoptosis in HeLa and HepG2 cells and is attributed mainly through a mitochondrial pathway by activation of caspases. The developmental and stage-specific expression of pierisin-6 mRNA was one thousand-fold increased from second to third instar larvae and gradually declined before pupation. CONCLUSION: Pierisin-6 represents a promising therapeutic approach for liver cancer patients.

2.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(6): 4663-4664, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-26643789

RESUMO

The complete mitogenome of Apis cerana cerana (Hymenoptera: Apidae: Apinae) was sequenced using Illumina NextSeq500 platform and found to be 15 831 bp long. The mitogenome contains 37 genes (13 PCGs, 22 tRNAs, and 2 rRNAs) and a control region. The base composition is biased towards A-T (83.9%). The control region is 498 bp long with polyT stretch and poly [TA (A)]n-like stretch. The phylogenetic tree constructed using concatenated PCGs showed that A. cerana cerana clustered with other cavity nesting Apis species.


Assuntos
Abelhas/genética , Genes de Insetos , Genes Mitocondriais , Genoma Mitocondrial , Animais , Composição de Bases , Abelhas/classificação , Evolução Molecular , Filogenia , Análise de Sequência de DNA
3.
Genom Data ; 6: 280-2, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26697399

RESUMO

In the present study, the near complete mitochondrial genome (mitogenome) of Junonia iphita (Lepidoptera: Nymphalidae: Nymphalinae) was determined to be 14,892 bp. The gene order and orientation are identical to those in other butterfly species. The phylogenetic tree constructed from the whole mitogenomes using the 13 protein coding genes (PCGs) defines the genetic relatedness of the two J. iphita species collected from two different regions. All the Junonia species clustered together, and were further subdivided into clade one consisting of J. almana and J. orithya and clade two comprising of the two J. iphita which were collected from Indo and Indochinese subregions separated by river barrier. Comparison between the two J. iphita sequences revealed minor variations and Single Nucleotide Polymorphisms were identified at 51 sites amounting to 0.4% of the entire mitochondrial genome.

4.
J Environ Biol ; 35(2): 369-76, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24665764

RESUMO

A systematic survey on Anopheline species abundance, bionomics and habitat preference was conducted for three years in Thenzawl, Mizoram. A scoop-net method was employed for larval collection and a local made killing-jar for adults. A total of 10 species Anopheles campestris (25.8%), An. nivipes (24.0%), An. vagus (20.6%), An. jamesii (15.1%), An. jeyporiensis (11.4%), An. maculatus (1.7%), An. philippinensis (0.7%), An. annularis (0.26%), An. sinensis (0.23%) and An. peditaeniatus (0.22%) were collected. The survey site having thick tall grasses, numerous rural-huts as residents, small to relatively larger ponds and very slow running water bodies well shaded from sunlight with floating aquatic plants provided the largest area for Anopheles larvae breeding and accounted for 40% of all Anopheles larva and 25.4% total Anopheles spp. collected. An. campestris (NSK01), maculatus (NSK04), philippinensis (NSK06), nivipes (NSK10) and jeyporiensis (NSK09) were strongly anthropogenic and endophagic while vagus (NSK18) and jamesii (NSK03) were found to be highly zoophilic and exophilic and An. peditaeniatus (NSK02), annularis (NSK07) and sinensis (NSK15) were found to be highly zoophilic. Because of its abundance and bionomics, An. campestris, jeyporiensis and nivipes may have played a role in malarial transmission throughout the year. This is the first study reported on Anopheline distribution and abundance in Thenzawl, Mizoram.


Assuntos
Anopheles/fisiologia , Demografia , Ecossistema , Animais , Índia , Densidade Demográfica , Fatores de Tempo
5.
Interdiscip Sci ; 5(2): 127-35, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23740394

RESUMO

Thirty nine Bt Cry1 subgroup protein sequences were retrieved from NCBI and analyzed for physicochemical properties, active site and relationship in relation to their variations in toxicity. Cry1 proteins were found to be hydrophilic and stable. SOSUI server predicted presence of two transmembrane regions in Ag and a single transmembrane region from Aa to Ae. EMBOSS PepWheel tool analysis of the transmembrane regions showed that there were 23 highly conserved residues towards the N terminal which are hydrophobic and more than half of the residues were neutrally charged. No signal peptide was detected which classifies the Cry1 group proteins as non-secretory proteins. Cry1 proteins have very high composition of neutral amino acids and might transform into negative charge after solubilization in alkaline environment (insect midgut). The negatively charged protein might misfold causing difficultly to digest and thereby be toxic to lepidopteran. Active sites of Cry1 proteins with more than 50% neutral amino acids showed wide insecticidal spectrum and further positive correlation (r = 0.7731) was observed between neutral amino acids and insect species affected (Y = -138.21 + 2.907X). Similarity of sequences was found between Cry1 proteins based on their high or low spectrum of insecticidal activity.


Assuntos
Bacillus thuringiensis/química , Proteínas de Bactérias/toxicidade , Endotoxinas/toxicidade , Proteínas Hemolisinas/toxicidade , Inseticidas/toxicidade , Sequência de Aminoácidos , Aminoácidos/química , Animais , Proteínas de Bactérias/química , Domínio Catalítico , Endotoxinas/química , Evolução Molecular , Proteínas Hemolisinas/química , Insetos/efeitos dos fármacos , Inseticidas/química , Dados de Sequência Molecular , Sinais Direcionadores de Proteínas , Alinhamento de Sequência , Solubilidade
6.
Parasitol Res ; 111(3): 1007-17, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22565399

RESUMO

Plant-based insecticides for vector control are urgently needed for Anopheles barbirostris, Culex quinquefasciatus, and Aedes albopictus which are the primary vectors of malaria, lymphatic filariasis, and dengue, respectively, in India and other South East Asian countries. In the present study, larvicidal, adulticidal, and repellent activities of acetone root bark extract of Hiptage benghalensis were tested against the larvae and adults of the three mosquito vectors. The acetone root bark extracts of H. benghalensis was more effective as larvicides with low LC(50) (11.15-16.78 ppm) and LT50 (1.25-4.84 h at 200 and 400 ppm) values. Results of log probit analysis (at 95 % confidence level) and regression analysis of crude acetone root bark extract of H. benghalensis revealed that lethal concentration (LC(50)) values gradually decreased with the exposure periods; lethal time (LT(50)) decreased with the concentration, and the mortality is positively correlated with the concentration. The order of susceptibility of the three mosquito species was as follows: A. albopictus > A. barbirostris > C. quinquefascitus. Biochemical changes were also evidenced in third instar larvae of three mosquito species following a sublethal exposure for 24 h. The level of sugar, glycogen, lipids, and proteins was significantly (P < 0.05) reduced in larvae treated with H. benghalensis. The acetone root bark extracts of H. benghalensis is less toxic to adults and repelled laboratory-reared female A. barbirostris, A. albopictus, and C. quinquefascitus with the short median protection times of 57.66-135, 72.41-134.16, and 47.66-93 min, respectively. The present investigation proves it as a potent larvicide against A. albopictus, A. barbirostris, and C. quinquefascitus, which can be recommended to control these mosquito species on its breeding site. However, further investigations are needed to confirm the lethal effects of H. benghalensis in field conditions and its impact on the nontarget organisms.


Assuntos
Aedes/efeitos dos fármacos , Anopheles/efeitos dos fármacos , Culex/efeitos dos fármacos , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Malpighiaceae/química , Animais , Bioensaio , Repelentes de Insetos/química , Inseticidas/química , Controle de Mosquitos , Casca de Planta/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Raízes de Plantas/química
7.
Interdiscip Sci ; 4(4): 296-301, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23354819

RESUMO

PROT-PROP is a computational tool to characterize 27 physicochemical properties of a protein along with its subcellular location (intra or extra) in a single-window application. Other significant features of this software include calculation of numerical values for hydrophobicity, hydrophilicity; composition of small and large amino acids; net hydrophobic content in terms of low/high; and Navie's algorithm to calculate theoretical pI. PROT-PROP is an easy-to-install platform independent implementation of JAVA under a user-friendly interface. It is a standalone version as a virtual appliance and source code for platforms supporting Java 1.5.0 and higher versions, and downloadable from the web http://www.mzu.edu.in/schools/biotechnology.html . PROT-PROP can run under Windows and Macintosh Operating Systems. PROT-PROP is distributed with its source code so that it may be adapted or customized, if desired.


Assuntos
Biologia Computacional/métodos , Proteínas/química , Análise de Sequência de Proteína , Software , Algoritmos , Aminoácidos , Bases de Dados de Proteínas , Interações Hidrofóbicas e Hidrofílicas , Internet , Linguagens de Programação , Proteínas/metabolismo , Interface Usuário-Computador
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