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2.
Hum Mol Genet ; 27(23): 4117-4134, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30452683

RESUMO

Pluripotent stem cells are invaluable resources to study development and disease, holding a great promise for regenerative medicine. Here we use human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) from patients with Huntington's disease (HD-iPSCs) to shed light into the normal function of huntingtin (HTT) and its demise in disease. We find that HTT binds ATF7IP, a regulator of the histone H3 methyltransferase SETDB1. HTT inhibits the interaction of the ATF7IP-SETDB1 complex with other heterochromatin regulators and transcriptional repressors, maintaining low levels of H3K9 trimethylation (H3K9me3) in hESCs. Loss of HTT promotes global increased H3K9me3 levels and enrichment of H3K9me3 marks at distinct genes, including transcriptional regulators of neuronal differentiation. Although these genes are normally expressed at low amounts in hESCs, HTT knockdown (KD) reduces their induction during neural differentiation. Notably, mutant expanded polyglutamine repeats in HTT diminish its interaction with ATF7IP-SETDB1 complex and trigger H3K9me3 in HD-iPSCs. Conversely, KD of ATF7IP in HD-iPSCs reduces H3K9me3 alterations and ameliorates gene expression changes in their neural counterparts. Taken together, our results indicate ATF7IP as a potential target to correct aberrant H3K9me3 levels induced by mutant HTT.


Assuntos
Proteína Huntingtina/genética , Doença de Huntington/genética , Metiltransferases de Proteína/genética , Fatores de Transcrição/genética , Diferenciação Celular/genética , Células-Tronco Embrionárias/metabolismo , Células-Tronco Embrionárias/patologia , Regulação da Expressão Gênica no Desenvolvimento , Técnicas de Silenciamento de Genes , Heterocromatina/genética , Histona Metiltransferases/genética , Humanos , Doença de Huntington/patologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Células-Tronco Pluripotentes Induzidas/patologia , Lentivirus/genética , Neurônios/metabolismo , Neurônios/patologia , Peptídeos/genética , Proteínas Repressoras
3.
Nat Commun ; 9(1): 2886, 2018 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-30038412

RESUMO

Induced pluripotent stem cells (iPSCs) undergo unlimited self-renewal while maintaining their potential to differentiate into post-mitotic cells with an intact proteome. As such, iPSCs suppress the aggregation of polyQ-expanded huntingtin (HTT), the mutant protein underlying Huntington's disease (HD). Here we show that proteasome activity determines HTT levels, preventing polyQ-expanded aggregation in iPSCs from HD patients (HD-iPSCs). iPSCs exhibit high levels of UBR5, a ubiquitin ligase required for proteasomal degradation of both normal and mutant HTT. Conversely, loss of UBR5 increases HTT levels and triggers polyQ-expanded aggregation in HD-iPSCs. Moreover, UBR5 knockdown hastens polyQ-expanded aggregation and neurotoxicity in invertebrate models. Notably, UBR5 overexpression induces polyubiquitination and degradation of mutant HTT, reducing polyQ-expanded aggregates in HD-cell models. Besides HTT levels, intrinsic enhanced UBR5 expression determines global proteostasis of iPSCs preventing the aggregation of misfolded proteins ensued from normal metabolism. Thus, our findings indicate UBR5 as a modulator of super-vigilant proteostasis of iPSCs.


Assuntos
Doença de Huntington/genética , Doença de Huntington/metabolismo , Células-Tronco Pluripotentes/metabolismo , Ubiquitina-Proteína Ligases/genética , Peptídeos beta-Amiloides/metabolismo , Animais , Caenorhabditis elegans , Diferenciação Celular , Variação Genética , Genótipo , Células HEK293 , Humanos , Proteína Huntingtina/genética , Proteína Huntingtina/metabolismo , Mutação , Neurônios/metabolismo , Peptídeos/metabolismo , Polimorfismo de Nucleotídeo Único , Complexo de Endopeptidases do Proteassoma/metabolismo , Desnaturação Proteica , Dobramento de Proteína , Proteômica , Proteostase
4.
Arch Gynecol Obstet ; 298(3): 655-661, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29971558

RESUMO

PURPOSE: We are conducting a prospective study trying to determine, in both sexes, the frequency of appearance of ectopic Leydig cells, their preferred location, their relationship with nerve structures and the possible causes of their appearance. METHODS: We have studied 86 cases that were removed according to different clinical indications for pathological study: uterine leyomiomas (n = 12), ovarian cystadenoma (n = 4), endometrial hyperplasia (n = 8), endometrial carcinoma (n = 12), cervical carcinoma (n = 4), seminoma (n = 4), fallopian tube ligatures (n = 24), vasectomies (n = 8), nonspecific orchiepididymitis (n = 2), and unknown (n = 8). RESULTS: We have observed ectopic Leydig cells in 13/86 cases (15.11%), 9/72 in the female samples (12.50%) and 4/14 in male samples (28.57%). The most frequent location was the mesosalpinx (4 of 13: 30.76%). CONCLUSIONS: These high figures lead us to believe that the ectopia of Leydig cells is not really a pathologic entity, but a finding related to specific functions yet to be determined.


Assuntos
Células Intersticiais do Testículo/citologia , Neoplasias Ovarianas/patologia , Testículo/citologia , Tubas Uterinas , Feminino , Humanos , Masculino , Estudos Prospectivos
5.
Sci Rep ; 8(1): 4092, 2018 03 06.
Artigo em Inglês | MEDLINE | ID: mdl-29511261

RESUMO

Human embryonic stem cells (hESCs) exhibit high levels of proteasome activity, an intrinsic characteristic required for their self-renewal, pluripotency and differentiation. However, the mechanisms by which enhanced proteasome activity maintains hESC identity are only partially understood. Besides its essential role for the ability of hESCs to suppress misfolded protein aggregation, we hypothesize that enhanced proteasome activity could also be important to degrade endogenous regulatory factors. Since E3 ubiquitin ligases are responsible for substrate selection, we first define which E3 enzymes are increased in hESCs compared with their differentiated counterparts. Among them, we find HECT-domain E3 ligases such as HERC2 and UBE3A as well as several RING-domain E3s, including UBR7 and RNF181. Systematic characterization of their interactome suggests a link with hESC identity. Moreover, loss of distinct up-regulated E3s triggers significant changes at the transcriptome and proteome level of hESCs. However, these alterations do not dysregulate pluripotency markers and differentiation ability. On the contrary, global proteasome inhibition impairs diverse processes required for hESC identity, including protein synthesis, rRNA maturation, telomere maintenance and glycolytic metabolism. Thus, our data indicate that high proteasome activity is coupled with other determinant biological processes of hESC identity.


Assuntos
Células-Tronco Embrionárias Humanas/enzimologia , Complexo de Endopeptidases do Proteassoma/análise , Ubiquitina-Proteína Ligases/análise , Ubiquitina/análise , Células Cultivadas , Perfilação da Expressão Gênica , Células-Tronco Embrionárias Humanas/química , Humanos , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Proteômica
6.
Cell Mol Life Sci ; 75(2): 275-290, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-28748323

RESUMO

Protein homeostasis, or proteostasis, is essential for cell function, development, and organismal viability. The composition of the proteome is adjusted to the specific requirements of a particular cell type and status. Moreover, multiple metabolic and environmental conditions challenge the integrity of the proteome. To maintain the quality of the proteome, the proteostasis network monitors proteins from their synthesis through their degradation. Whereas somatic stem cells lose their ability to maintain proteostasis with age, immortal pluripotent stem cells exhibit a stringent proteostasis network associated with their biological function and intrinsic characteristics. Moreover, growing evidence indicates that enhanced proteostasis mechanisms play a central role in immortality and cell fate decisions of pluripotent stem cells. Here, we will review new insights into the melding fields of proteostasis and pluripotency and their implications for the understanding of organismal development and survival.


Assuntos
Estresse do Retículo Endoplasmático , Células-Tronco Pluripotentes/metabolismo , Proteoma/metabolismo , Proteostase , Animais , Diferenciação Celular , Sobrevivência Celular , Humanos , Modelos Biológicos , Células-Tronco Pluripotentes/citologia , Resposta a Proteínas não Dobradas
8.
Int J Mol Sci ; 18(7)2017 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-28753941

RESUMO

Huntington's disease (HD) is a fatal neurodegenerative disorder characterized by motor dysfunction, cognitive deficits and psychosis. HD is caused by mutations in the Huntingtin (HTT) gene, resulting in the expansion of polyglutamine (polyQ) repeats in the HTT protein. Mutant HTT is prone to aggregation, and the accumulation of polyQ-expanded fibrils as well as intermediate oligomers formed during the aggregation process contribute to neurodegeneration. Distinct protein homeostasis (proteostasis) nodes such as chaperone-mediated folding and proteolytic systems regulate the aggregation and degradation of HTT. Moreover, polyQ-expanded HTT fibrils and oligomers can lead to a global collapse in neuronal proteostasis, a process that contributes to neurodegeneration. The ability to maintain proteostasis of HTT declines during the aging process. Conversely, mechanisms that preserve proteostasis delay the onset of HD. Here we will review the link between proteostasis, aging and HD-related changes.


Assuntos
Proteína Huntingtina/genética , Proteína Huntingtina/metabolismo , Proteostase , Envelhecimento/metabolismo , Chaperonas de Histonas , Humanos , Proteína Huntingtina/química , Doença de Huntington/genética , Chaperonas Moleculares/metabolismo , Mutação , Dobramento de Proteína , Proteólise
9.
FEBS J ; 284(3): 391-398, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-27398614

RESUMO

Embryonic stem cells (ESCs) exhibit a striking ability to replicate continuously in the absence of senescence. Despite the knowledge gained into ESC biology and cell reprogramming, the mechanisms that regulate their pluripotency, self-renewal, and differentiation remain largely unknown. Recently, cumulative evidence has highlighted the importance of protein homeostasis, or proteostasis, in the maintenance of ESC function. These findings indicate that ESCs exhibit intrinsic differences in the regulation and activity of key nodes of the proteostasis network such as global protein synthesis, folding, and degradation rates. Here, we review new insights into proteostasis of ESCs and the questions raised by these findings. In addition, we discuss the potential of these discoveries to be applied into aging and cancer research.


Assuntos
Envelhecimento/genética , Células-Tronco Embrionárias/metabolismo , Homeostase/genética , Neoplasias/metabolismo , Proteoma/metabolismo , Envelhecimento/metabolismo , Animais , Diferenciação Celular , Proliferação de Células , Reprogramação Celular , Células-Tronco Embrionárias/citologia , Humanos , Terapia de Alvo Molecular , Neoplasias/genética , Neoplasias/patologia , Neoplasias/terapia , Complexo de Endopeptidases do Proteassoma/metabolismo , Biossíntese de Proteínas , Dobramento de Proteína , Estabilidade Proteica , Proteólise , Proteoma/genética
10.
Nat Commun ; 7: 13649, 2016 11 28.
Artigo em Inglês | MEDLINE | ID: mdl-27892468

RESUMO

Human embryonic stem cells can replicate indefinitely while maintaining their undifferentiated state and, therefore, are immortal in culture. This capacity may demand avoidance of any imbalance in protein homeostasis (proteostasis) that would otherwise compromise stem cell identity. Here we show that human pluripotent stem cells exhibit enhanced assembly of the TRiC/CCT complex, a chaperonin that facilitates the folding of 10% of the proteome. We find that ectopic expression of a single subunit (CCT8) is sufficient to increase TRiC/CCT assembly. Moreover, increased TRiC/CCT complex is required to avoid aggregation of mutant Huntingtin protein. We further show that increased expression of CCT8 in somatic tissues extends Caenorhabditis elegans lifespan in a TRiC/CCT-dependent manner. Ectopic expression of CCT8 also ameliorates the age-associated demise of proteostasis and corrects proteostatic deficiencies in worm models of Huntington's disease. Our results suggest proteostasis is a common principle that links organismal longevity with hESC immortality.


Assuntos
Caenorhabditis elegans/fisiologia , Chaperonina com TCP-1/metabolismo , Longevidade , Células-Tronco Pluripotentes/metabolismo , Proteostase , Animais , Diferenciação Celular , Técnicas de Silenciamento de Genes , Células HEK293 , Células-Tronco Embrionárias Humanas/citologia , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Mutação/genética , Fenótipo , Agregados Proteicos , Subunidades Proteicas/metabolismo , Estresse Fisiológico
11.
Biometals ; 29(5): 935-44, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27567902

RESUMO

Accurate quantification depends on normalization of the measured gene expression data. In particular, gene expression studies with exposure to metals are challenging due their toxicity and redox-active properties. Here, we assessed the stability of potential reference genes in three cell lines commonly used to study metal cell metabolism: Caco-2 (colon), HepG2 (liver) and THP-1 (peripheral blood) under copper (Cu) or zinc (Zn) exposure. We used combined statistical tools to identify the best reference genes from a set of eleven candidates, which included traditional "housekeeping" genes such as GAPDH and B-ACTIN, in cell lines exposed to high and low, Zn and Cu concentrations. The expression stabilities of ATP5B (ATP synthase) and CYC1 (subunits of the cytochrome) were the highest considering the effect of Zn and Cu treatments whereas SDHA (succinate dehydrogenase) was found to be the most unstable gene. Even though the transcriptional effect of Zn and Cu is very different in term of redox properties, the same best reference genes were identified when Zn or Cu treatments were analyzed together. Our results indicate that ATP5B/CYC1 are the best candidates for reference genes after metal exposure, which can be used as a suitable starting point to evaluate gene expression with other metals or in different cell types in human models.


Assuntos
Cobre/farmacologia , Grupo dos Citocromos c/genética , ATPases Mitocondriais Próton-Translocadoras/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Zinco/farmacologia , Linhagem Celular , Grupo dos Citocromos c/metabolismo , Grupo dos Citocromos c/normas , Perfilação da Expressão Gênica , Humanos , ATPases Mitocondriais Próton-Translocadoras/metabolismo , ATPases Mitocondriais Próton-Translocadoras/normas , Reação em Cadeia da Polimerase em Tempo Real/normas , Padrões de Referência
12.
J Cell Physiol ; 229(5): 607-19, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24446197

RESUMO

Copper is an essential cofactor of complex IV of the electron transfer chain, and it is directly involved in the generation of mitochondrial membrane potential. Its deficiency induces the formation of ROS, large mitochondria and anemia. Thus, there is a connection between copper metabolism and bioenergetics, mitochondrial dynamics and erythropoiesis. Copper depletion might end in cellular apoptosis or necrosis. However, before entering into those irreversible processes, mitochondria may execute a series of adaptive responses. Mitochondrial adaptive responses (MAR) may involve multiple and diverse mechanisms for preserving cell life, such as mitochondrial dynamics, OXPHOS remodeling and bioenergetics output. In this study, a mild copper deficiency was produced in an animal model through intraperitoneal injections of bathocuproine disulfonate in order to study the MAR. Under these conditions, a new type of mitochondrial morphology was discovered in the liver. Termed the "butternut squash" mitochondria, it coexisted with normal and swollen mitochondria. Western blot analyses of mitochondrial dynamics proteins showed an up-regulation of MFN-2 and OPA1 fusion proteins. Furthermore, isolated liver mitochondria displayed OXPHOS remodeling through a decrease in supercomplex activity with a concomitant increase at an individual level of complexes I and IV, higher respiratory rates at complex I and II levels, higher oligomycin-insensitive respiration, and lower respiratory control ratio values when compared to the control group. As expected, total ATP and ATP/ADP values were not significantly different, since animal's health was not compromised. As a whole, these results describe a compensatory and adaptive response of metabolism and bioenergetics under copper deprivation.


Assuntos
Adaptação Fisiológica/fisiologia , Cobre/deficiência , Metabolismo Energético/fisiologia , Mitocôndrias Hepáticas/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Fosforilação Oxidativa , Trifosfato de Adenosina/metabolismo , Animais , Quelantes/farmacologia , Cobre/metabolismo , Masculino , Camundongos , Fenantrolinas/farmacologia , Espécies Reativas de Oxigênio
13.
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-987114

RESUMO

El presente artículo surge de la revisión documental del concepto violencias escolares en bases de datos, artículos de revista, bibliotecas y demás medios de información, dentro de la investigación: "Violencias escolares presentes en las ins tituciones educativas católicas. Una aproximación a los discursos y prácticas en el Colegio Parroquial Emaús de la ciudad de Medellín", de la Maestría en Intervenciones Psicosociales de la Fundación Universitaria Luis Amigó. La búsqueda documental arrojó tres categorías: Bullying, violencias de los escolares y conflicto escolar, que alimentaron la construcción en torno a la violencia escolar, evidenciando la necesidad de pluralizar el concepto, más allá de tipificarlo en el bullying, para abarcar sus diversas manifestaciones.


This article comes from the review of documents in databases, journal articles, libraries and other ways, in research "School Violence present in Catholic educational institutions. An approach to the discourses and practices in Emaús Parish School of Medellin", within the Maestría en Intervenciones Psicosociales de la Fundación Universitaria Luis Amigó. During the search we found three theoretical framework: Bullying, School Violence, and Conflict School, which fueled construction around school violence evidencing the need to pluralize the concept beyond typify in Bullying, to cover its various manifestations.


Assuntos
Humanos , Bullying , Estudantes/psicologia , Psicologia do Adolescente , Agressão/ética
14.
Biometals ; 26(6): 1033-40, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24170205

RESUMO

Copper is an essential micronutrient that functions as an enzymatic cofactor in a wide range of cellular processes. Although adequate Cu levels are essential for normal metabolism, excess Cu can be toxic to cells. Cellular responses to copper deficiency and overload involve changes in the expression of genes directly and indirectly involved in copper metabolism. However little is known on the effect of physiological copper concentration on gene expression changes. In the current study we aimed to establish whether the expression of genes encoding enzymes related to cholesterol (hmgcs1, hmgcr, fdft) and fatty acid biosynthesis and LDL receptor can be induced by an iso-physiological copper concentration. The iso-physiological copper concentration was determined as the bioavailable plasmatic copper in a healthy adult population. In doing so, two blood cell lines (Jurkat and THP-1) were exposed for 6 or 24 h to iso- or supraphysiological copper concentrations. Our results indicated that in cells exposed to an iso-physiological copper concentration the early induction of genes involved in lipid metabolism was not mediated by copper itself but by the modification of the cellular redox status. Thus our results contributed to understand the involvement of copper in the regulation of cholesterol metabolism under physiological conditions.


Assuntos
Colesterol/biossíntese , Cobre/farmacologia , Expressão Gênica/efeitos dos fármacos , Histidina/análogos & derivados , Compostos Organometálicos/farmacologia , RNA Mensageiro/genética , Colesterol/genética , Farnesil-Difosfato Farnesiltransferase/genética , Farnesil-Difosfato Farnesiltransferase/metabolismo , Ácido Graxo Sintase Tipo I/genética , Ácido Graxo Sintase Tipo I/metabolismo , Histidina/farmacologia , Humanos , Hidroximetilglutaril-CoA Redutases/genética , Hidroximetilglutaril-CoA Redutases/metabolismo , Hidroximetilglutaril-CoA Sintase/genética , Hidroximetilglutaril-CoA Sintase/metabolismo , Células Jurkat , Metabolismo dos Lipídeos/efeitos dos fármacos , Oxirredução , RNA Mensageiro/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptores de LDL/genética , Receptores de LDL/metabolismo
15.
Arch Esp Urol ; 60(6): 703-6, 2007.
Artigo em Espanhol | MEDLINE | ID: mdl-17847750

RESUMO

OBJECTIVE: Report of one case of desmoid tumor in a patient who had been treated of a testicular seminoma 26 months before, with excision of a retroperitoneal mass and chemotherapy. On followup he presented with a mesenteric abdominal mass which was clinically labeleled as a recurrence of the seminoma. RESULTS: Histologically it was reported as a mesenteric desmoid tumor. Differential diagnosis with gastrointestinal stromal tumor was performed with immunohistochemical studies. CONCLUSIONS: Desmoid tumor is rare. There are few cases reported in patients with history of previous testicular tumor. It should be included in the differential diagnosis of testicular tumor recurrences.


Assuntos
Fibromatose Agressiva/diagnóstico , Mesentério , Neoplasias Peritoneais/diagnóstico , Seminoma/diagnóstico , Neoplasias Testiculares/diagnóstico , Adulto , Diagnóstico Diferencial , Humanos , Masculino , Recidiva Local de Neoplasia/diagnóstico
16.
Arch. esp. urol. (Ed. impr.) ; 60(6): 703-705, jul.-ago. 2007. ilus
Artigo em Espanhol | IBECS | ID: ibc-055533

RESUMO

Objetivo: Presentar un caso de un tumor desmoide en un paciente tratado de un seminoma testicular que simulaba una recidiva del tumor testicular. Método: Presentamos el caso de un paciente de 41 años, tratado de un seminoma testicular 26 meses antes, mediante extirpación de una masa testicular retroperitoneal y quimioterapia, que presenta en el seguimiento, una masa abdominal mesentérica que se etiquetó clínicamente de recidiva de seminoma. Resultado: Histológicamente se informa de tumor desmoide mesentérico. Se hace diagnóstico diferencial con un tumor de estroma gastrointestinal mediante el estudio inmunohistoquímico. Conclusiones: El tumor desmoide es un tumor raro. Se han descrito pocos casos en pacientes afectos previamente de tumor testicular. Debe incluirse en el diagnóstico diferencial de las recidivas por tumor testicular (AU)


Objective: Report of one case of desmoid tumor in a patient who had been treated of a testicular seminoma 26 months before, with excision of a retroperitoneal mass and chemotherapy. On follow-up he presented with a mesenteric abdominal mass which was clinically labeleled as a recurrence of the seminoma. Results: Histologically it was reported as a mesenteric desmoid tumor. Differential diagnosis with gastrointestinal stromal tumor was performed with immunohistochemical studies. Conclusions: Desmoid tumor is rare. There are few cases reported in patients with history of previous testicular tumor. It should be included in the differential diagnosis of testicular tumor recurrences (AU)


Assuntos
Masculino , Adulto , Humanos , Fibromatose Agressiva/etiologia , Seminoma/complicações , Neoplasias Testiculares/complicações , Metástase Neoplásica/diagnóstico , Fibromatose Agressiva/diagnóstico , Fibromatose Agressiva/patologia , Seminoma/tratamento farmacológico , Seminoma/cirurgia , Diagnóstico Diferencial , Mesentério/patologia , Neoplasias Testiculares/tratamento farmacológico , Neoplasias Testiculares/cirurgia , Metástase Neoplásica/patologia
17.
Arch Esp Urol ; 57(6): 657-60, 2004.
Artigo em Espanhol | MEDLINE | ID: mdl-15382446

RESUMO

OBJECTIVES: To report one case of an exceptional benign prostatic pathology and its differential diagnosis with malignant tumors. METHODS: 67-year-old male who suffers an acute urinary retention requiring bladder catheterization and subsequent negative catheter removal tests. Digital rectal examination showed a small prostate, adenomatous, without nodules. PSA was 1.01 ng/ml. The patient underwent transurethral resection of the prostate because of the persistence of urinary retention. RESULTS: Pathologic study reported a hypercellular stroma, with a perivascularly distributed inflammatory infiltrate and myxoid stromal background with slightly atypical fusiform cells. Immunohistochemical studies showed positive staining of fusiform cells for vimentin and histiocytes in the lesion for CD68, and negative staining for cytokeratin. The final diagnosis was prostatic inflammatory pseudotumor. CONCLUSIONS: In spite of being an unfrequent presentation it is important to take this benign lesion under consideration to avoid unnecessary aggressive radical complementary treatments.


Assuntos
Granuloma de Células Plasmáticas/patologia , Doenças Prostáticas/patologia , Idoso , Diagnóstico Diferencial , Granuloma de Células Plasmáticas/cirurgia , Humanos , Masculino , Doenças Prostáticas/cirurgia , Ressecção Transuretral da Próstata
18.
Arch Esp Urol ; 56(5): 536-8, 2003 Jun.
Artigo em Espanhol | MEDLINE | ID: mdl-12918315

RESUMO

OBJECTIVES: We present a case of adult polycystic kidney disease, also known as autosomal dominant polycystic kidney, complicated by infection of the cysts and the formation of gas within them. METHODS/RESULTS: A 59 year old patient diagnosed of adult polycystic kidney disease with chronic renal failure on treatment with haemodialysis, who presented sepsis secondary to infection of the renal cysts. The CT scan demonstrated the presence of gas within the cysts and the microbiology revealed E. coli in one of them. Urgent nephrectomy was performed. A histological specimen of the excised organ is also presented. CONCLUSIONS: Infection of one or more cysts in adult polycystic kidney disease is a rare and serious complication which may require immediate nephrectomy, particularly if gas appears within the cysts.


Assuntos
Infecções por Escherichia coli/complicações , Rim Policístico Autossômico Dominante/complicações , Pielite/complicações , Diabetes Mellitus Tipo 2/complicações , Suscetibilidade a Doenças , Infecções por Escherichia coli/cirurgia , Gases , Humanos , Cirrose Hepática Alcoólica/complicações , Masculino , Pessoa de Meia-Idade , Nefrectomia , Rim Policístico Autossômico Dominante/cirurgia , Pielite/cirurgia , Sepse/etiologia
19.
Arch. esp. urol. (Ed. impr.) ; 56(5): 536-538, jun. 2003.
Artigo em Espanhol | IBECS | ID: ibc-25082

RESUMO

OBJETIVOS: Presentamos el caso de un riñón poliquístico del adulto o riñón poliquístico autosómico dominante, complicado con la infección de los quistes y con formación de gas dentro de ellos. RESULTADOS: Paciente de 59 años de edad diagnosticado de enfermedad renal poliquística del adulto con insuficiencia renal crónica en tratamiento con hemodiálisis, que presentó sepsis secundaria a la infección de los quistes renales. El TAC demostró la presencia de gas dentro de los quistes y el estudio microbiológico demostró la presencia de E. coli en el interior de uno de ellos. Se realizó una nefrectomía urgente. Se muestra además un detalle microscópico de la pieza de nefrectomía. CONCLUSIONES: La infección de uno o varios de los quistes de un riñón poliquístico del adulto es una complicación poco frecuente y grave que puede requerir la nefrectomía inmediata, especialmente si dentro de los quistes aparece gas. (AU)


Assuntos
Pessoa de Meia-Idade , Masculino , Humanos , Rim Policístico Autossômico Dominante , Sepse , Nefrectomia , Pielite , Suscetibilidade a Doenças , Cirrose Hepática Alcoólica , Infecções por Escherichia coli , Gases , Diabetes Mellitus Tipo 2
20.
Arch Esp Urol ; 55(5): 547-51, 2002 Jun.
Artigo em Espanhol | MEDLINE | ID: mdl-12174423

RESUMO

OBJECTIVE: To present a case of transitional cell carcinoma of the bladder with diffuse intrasinusoidal metastases to the liver that presented as fulminant hepatic failure. METHODS/RESULTS: A 65-year-old patient who presented at the emergency department of this hospital with fever and pain in the right hypochondrium and flank is described. Three months previously the patient had undergone operation in our department for a recurrence of a tumor affecting the bladder and urethra (Tis of the bladder and T1 GII of the prostatic urethra). The blood tests on admission were practically normal but showed alterations from the twelfth day onwards, suggesting acute liver failure in the differential diagnosis; the patient died 21 days later. The ultrasound and CT scans showed hepatomegaly with multiple heterogeneous areas which were not visible three months earlier and with no space-occupying lesions. At autopsy, the liver was found to be enlarged, with no macroscopic metastatic nodules. Microscopic examination revealed massive tumoral infiltration of the hepatic sinusoids with diffuse replacement of the hepatocytes. CONCLUSIONS: Secondary, metastatic liver cancer usually presents as multiple nodular lesions and only vary on very rare occasions as a diffuse infiltration leading to acute hepatic failure. This case provides the first description of the autopsy findings in a bladder transitional cell carcinoma with diffuse intrasinusoidal metastases to the liver.


Assuntos
Carcinoma de Células de Transição/secundário , Falência Hepática/etiologia , Neoplasias Hepáticas/secundário , Neoplasias da Bexiga Urinária/patologia , Idoso , Carcinoma de Células de Transição/complicações , Evolução Fatal , Humanos , Neoplasias Hepáticas/complicações , Masculino , Fumar
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