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1.
HNO ; 68(1): 8-13, 2020 Jan.
Artigo em Alemão | MEDLINE | ID: mdl-31511908

RESUMO

BACKGROUND: While an abundant number of studies concerning tobacco smoke and chewing tobacco show carcinogenic potential, there is little data on the consequences of snuff, especially on the cellular level. Therefore, the mutagenic effect of snuff is difficult to estimate and the WHO assessment of snuff being not carcinogenic is based on very limited data. OBJECTIVES: This paper investigates the potential cytotoxic and genotoxic effects of snuff on human lymphocytes and nasal mucosa cells. MATERIALS AND METHODS: Two types of snuff were used: one without menthol and one with a high degree of menthol. The necessary nasal mucosa cells and lymphocytes were collected from 10 subjects undergoing nasal obstruction surgery and incubated for one hour with a snuff-DMSO mixture (range 0.01-2000 µg/ml). Methods included the trypan blue test, the comet assay, and the micronucleus test. RESULTS: The trypan blue test showed no decrease in cell viability for either cell type. The comet assay revealed a significant increase in the Olive Tail Moment for lymphocytes starting at 100 µg/ml and at 1000 µg/ml for nasal mucosa cells. There was no significant increase in micronuclei according to the micronucleus test. No differences between these two types of tobacco were observed. CONCLUSION: The present study demonstrated genotoxic damage, such as DNA strand breaks, which may be repaired, but no non-repairable elevated micronuclei. The present findings cast doubts on the WHO assessment that snuff is not carcinogenic. However, for a sound assessment of the risk potential of snuff, further research on various genotoxic endpoints in human cells is warranted.


Assuntos
Linfócitos , Mucosa Nasal , Tabaco sem Fumaça , Ensaio Cometa , Dano ao DNA , Humanos , Linfócitos/efeitos dos fármacos , Mucosa Nasal/efeitos dos fármacos , Tabaco sem Fumaça/efeitos adversos
2.
Anaesthesia ; 74(12): 1572-1579, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31508815

RESUMO

Adenotonsillectomies are commonly performed procedures and sleep-disordered breathing is becoming increasingly important as an indication for surgery. Because of the higher risks in patients with obstructive sleep apnoea, the required level of postoperative care for these patients is currently under discussion, and better identification of patients at risk may reduce unnecessary postoperative monitoring. To evaluate the influence of obstructive sleep apnoea, and other risk factors, on peri-operative complications in children requiring adenotonsillectomy, we performed a retrospective case-control study that included 1995 patients treated between January 2009 and June 2017. In our analysis, young age (OR 3.8, 95%CI 2.1-7.1), low body weight (OR 2.6, 95%CI 1.5-4.4), obstructive sleep apnoea (OR 2.4, 95%CI 1.5-3.8), pre-existing craniofacial or syndromal disorders (OR 2.3, 95%CI 1.4-3.8) and adenotonsillectomy, compared with adenoidectomy alone, (OR 7.9, 95%CI 4.7-13.1) were identified as risk factors for complications during or after surgery, p < 0.001. All 13 patients suffering from complications more than 3 h postoperatively had obstructive sleep apnoea plus at least one more of these risk factors. Patients at risk of postoperative complications can therefore be identified by several criteria pre-operatively, and should be monitored postoperatively using pulse oximetry overnight. For all other patients, postoperative observation on a surgical ward without extra monitoring is sufficient. Admission to paediatric intensive care should be reserved for patients suffering serious intra-operative complications.


Assuntos
Adenoidectomia/efeitos adversos , Complicações Pós-Operatórias/epidemiologia , Tonsilectomia/efeitos adversos , Adenoidectomia/estatística & dados numéricos , Adolescente , Fatores Etários , Peso Corporal , Estudos de Casos e Controles , Criança , Pré-Escolar , Anormalidades Craniofaciais/complicações , Feminino , Humanos , Lactente , Masculino , Monitorização Fisiológica , Oximetria , Estudos Retrospectivos , Fatores de Risco , Síndromes da Apneia do Sono/complicações , Síndromes da Apneia do Sono/epidemiologia , Tonsilectomia/estatística & dados numéricos
3.
Colloids Surf B Biointerfaces ; 181: 1019-1025, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382329

RESUMO

Nanoparticles, such as TiO2 particles, have a great potential for biomedical applications due to their ultra-small size and large specific surface area. However, their detection within cells is to date more than challenging. Thus, implementing fluorescence properties to nanoparticles via their controlled functionalisation with an organic chromophore is an original and efficient strategy to enable their visualization. In this work, a silylated coupling agent bearing a luminescent rhodamine B group was synthesised and grafted on the surface of anatase nanoparticles. The successful functionalisation was demonstrated via zeta potential, dynamic light scattering and diffuse reflectance infrared Fourier transform analyses. Remarkably, the obtained luminescent TiO2 particles showed an improved photocatalytic activity compared to the pristine nanoparticles. Both, as-synthesised and functionalised TiO2 nanoparticles samples appear to be non-toxic towards malignant and non-malignant cells. Moreover, the detection of the functionalised particles within cultured cells was proven to be easy and efficient via confocal fluorescence microscopy.


Assuntos
Fluorescência , Nanopartículas/química , Compostos de Organossilício/química , Processos Fotoquímicos , Titânio/química , Pesquisa Biomédica , Catálise , Microscopia Confocal , Microscopia de Fluorescência , Compostos de Organossilício/síntese química , Tamanho da Partícula , Rodaminas/química , Propriedades de Superfície
4.
HNO ; 67(11): 819-824, 2019 Nov.
Artigo em Alemão | MEDLINE | ID: mdl-31119330

RESUMO

BACKGROUND: Primary immunodeficiency is a rare disease of humoral and cellular immune defense, which can lead to severe and recurrent infections of different organs. The diagnosis of this disease is often difficult, and its early identification is necessary for adequate treatment and control. OBJECTIVE: This study aimed to analyze ear, nose, and throat (ENT) infections in adults and children with a primary immunodeficiency. We attempted to characterize possible warning signs that should trigger an immunologic diagnostic workup. MATERIALS AND METHODS: The current study comprised a retrospective case series of patients with primary immunodeficiencies. The type of immunodeficiency and the number of ENT infections were recorded. RESULTS: A total of 85 Patients were included in the study. 56 patients (66%) had an acute exacerbation of chronic rhinosinusitis (n = 28), cervical lymphadenitis (n = 16), acute tonsillitis (n = 14), and acute otitis media (n = 6). Reporting detailed information about the frequencies and dates of infections was not possible, due to the retrospective nature of the analysis. CONCLUSION: The prevalence of ENT infections in patients with a primary immunodeficiency is increased compared to the normal population. For the ENT specialist, these findings underline the necessity of including primary immunodeficiency in the differential diagnosis and initiating targeted diagnostic methods where indicated. Interdisciplinary collaboration with rheumatologists and immunologists is highly recommended, particularly for pediatric patients.


Assuntos
Síndromes de Imunodeficiência , Otite Média , Abscesso Peritonsilar , Sinusite , Adulto , Criança , Humanos , Síndromes de Imunodeficiência/complicações , Síndromes de Imunodeficiência/diagnóstico , Otite Média/imunologia , Abscesso Peritonsilar/imunologia , Estudos Retrospectivos , Sinusite/imunologia
6.
Colloids Surf B Biointerfaces ; 143: 7-14, 2016 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-26998862

RESUMO

Stable, non-agglomerated TiO2 nanoparticle (NP) dispersions are a crucial requirement for an accurate NP dosing in in vitro and in vivo experiments. In this study self-synthesised TiO2 NPs were stabilised in three different cell culture media (DMEM, RPMI, BEGM) with the help of stabilising agents. Cell culture tested stabilisers (bovine serum albumin, fetal bovine serum) were compared to non-tested commercial products which are commonly utilized in the cement industry (Melflux(®) 4930 F, Melpers(®) 4343, Sika(®) ViscoCrete(®)-10110178). For a quantitative evaluation and comparison of the degree of stabilisation, a sedimentation study using UV absorbance spectroscopy was carried out and the agglomerate size was measured via dynamic light scattering. The cytotoxicity of the novel surfactants and stabilised NPs was examined in a head and neck squamous cell carcinoma-derived FaDu cell line and in human mesenchymal stem cells. We successfully stabilised TiO2 NPs with Melflux(®) 4930 F in each cell culture medium, achieving perfect stability over at least one day and agglomerate sizes of less than 100nm, while the cytotoxicity of the NPs was not affected.


Assuntos
Ácidos Carboxílicos/química , Éteres/química , Nanopartículas Metálicas/química , Titânio/química , Animais , Ácidos Carboxílicos/farmacologia , Bovinos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Meios de Cultura/química , Éteres/farmacologia , Floculação/efeitos dos fármacos , Humanos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/fisiologia , Polimerização , Cultura Primária de Células , Soroalbumina Bovina/química , Soluções , Tensoativos/química , Titânio/farmacologia
7.
Oncol Rep ; 35(1): 219-26, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26530463

RESUMO

Human mesenchymal stem cells (hMSCs) have been applied therapeutically in numerous clinical trials. The pro-angiogenic effects of hMSCs, as well as their strong tumor tropism, have been shown both in vitro and in vivo. Some studies suggest using hMSCs as potential drug-carriers for tumor therapy. In previous investigations by our group, the pro-tumorigenic effects of hMSCs on head and neck squamous cell carcinoma (HNSCC) were shown. However, the influence of hMSCs on tumor vascularization as well as the possibility of its inhibition are yet to be elucidated. The cytokine patterns of the HNSCC cell line FaDu, native hMSCs (hMSCs-nat), hMSCs differentiated into adipocytes (hMSCs-adip) and osteocytes (hMSCs-ost) were evaluated. Human umbilical vein endothelial cells (HUVECs) were co-cultured with FaDu cells, hMSCs-nat, hMSCs-adip and hMSCs-ost. The capillary tube formation assay was applied. Furthermore, the migration capability of hMSCs-nat, hMSCs-adip and hMSCs-ost towards FaDu cells was measured in a Transwell system. Spheroids were cultured from hMSCs-nat, FaDu cells and DiI-labeled HUVECs. FaDu cells, hMSCs-nat, hMSCs-adip and hMSCs-ost released a wide range of cytokines and growth factors, e.g., IL-6, IL-8, IL-10, GRO and MCP. In the capillary tube formation assay, HUVECs generated significantly longer tubes after co-cultivation with hMSCs-nat as compared to HUVECs alone and FaDu. Differentiation into adipocytes and osteocytes counteracted the tube formation. The adipogenic differentiation did not alter hMSC motility, whereas osteogenic differentiation significantly inhibited hMSC migration. Generation of multi-cellular spheroids from hMSCs-nat, FaDu cells and DiI-labeled HUVECs was possible. Florescence microscopy revealed that all HUVECs were present in the spheroid core. Taken together, hMSCs-nat have a pro-angiogenic effect. The effects are counteracted by the differentiation of hMSCs towards osteogenic and adipogenic lineages. The differentiation of stem cells into different lineages may be a promising solution to generating carriers for cancer therapy without pro-tumorigenic properties.


Assuntos
Adipogenia , Células-Tronco Mesenquimais/citologia , Neovascularização Fisiológica , Osteogênese , Carcinoma de Células Escamosas/irrigação sanguínea , Carcinoma de Células Escamosas/imunologia , Diferenciação Celular , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Células Cultivadas , Técnicas de Cocultura , Citocinas/metabolismo , Neoplasias de Cabeça e Pescoço/irrigação sanguínea , Neoplasias de Cabeça e Pescoço/imunologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Técnicas In Vitro , Células-Tronco Mesenquimais/imunologia , Esferoides Celulares/citologia , Esferoides Celulares/imunologia , Carcinoma de Células Escamosas de Cabeça e Pescoço
8.
Laryngorhinootologie ; 95(5): 336-42, 2016 May.
Artigo em Alemão | MEDLINE | ID: mdl-26669579

RESUMO

BACKGROUND: The Direct-Drive-Simulation (DDS) tends to simulate the sound quality of hearing with the active middle ear implant Vibrant Soundbridge(®) (VSB). Up to now a scientific evaluation of the validity is missing. Furthermore, the test procedure has not been described yet. Aim of this study was to evaluate the test validity and to describe the test realization in detail. MATERIAL AND METHODS: 10 patients evaluated their sound impression on scales from 1 to 10 concerning sound quality during DDS, postoperative free field testing at least 3 month after the first fitting of the VSB and in the everyday life situation. 3 patients were implanted bilaterally. Together, 36 data sets could be analyzed. RESULTS: Coupling of the Floating Mass Transducer (FMT), which was placed inside of a silicone probe during DDS was successful in all cases. In 11 out of 13 cases the coupling quality was judged as "good" an only in 2 cases as "medium". None of the patients needed local anesthesia. Comparing the evaluation of the sound impression during DDS preoperatively, and with the implanted VSB in free field testing and in everyday life no significant differences were found. CONCLUSION: The DDS offers the possibility of a realistic preoperative sound simulation of the "VSB-hearing" in case of sensorineural hearing loss. Thus, the test is supposed to facilitate the patient's decision towards possible treatment options. The specialist gets additional information regarding the indication especially when audiologic indication criteria are critical. The DDS should be a basic part of the preoperative diagnostic prior to VSB-implantation.


Assuntos
Simulação por Computador , Prótese Ossicular , Espectrografia do Som/instrumentação , Percepção da Fala , Transdutores , Qualidade da Voz , Adolescente , Adulto , Idoso , Discos Compactos , Desenho de Equipamento , Feminino , Seguimentos , Humanos , Percepção Sonora , MP3-Player , Masculino , Pessoa de Meia-Idade , Música , Satisfação do Paciente , Valor Preditivo dos Testes , Inquéritos e Questionários , Adulto Jovem
9.
HNO ; 62(6): 432, 434-8, 2014 Jun.
Artigo em Alemão | MEDLINE | ID: mdl-24916351

RESUMO

Nanomaterials are not just used in various areas of scientific research, but are increasingly found in consumer products. Particularly the cosmetic and textile industries, as well as the medical branch benefit from the specific chemical and physical properties of nanoparticles (NPs). However, the knowledge base concerning the potential health hazards that nanomaterials hold for humans is far from complete. NPs mainly enter the organism via the lungs or the gastrointestinal tract, where they can accumulate. Transcutaneous penetration is most unlikely in the case of healthy skin. Chronic inflammatory reactions of the airways are particularly relevant in the context of potential risks to human health. Evidence for a geno- and cytotoxic potential of some of the most frequently used NPs is available from cell culture and animal experiments. Therefore, the risk of NP-induced cancerogenesis cannot be ruled out. Currently available nanotoxicological data is partly contradictory, due to differing characteristics of the tested substances and variable experimental settings. Long-term studies using continuous NP exposure in consumer-relevant dosages are needed. Additionally, the molecular mechanisms of NP-induced toxicity have to be elucidated in detail.


Assuntos
Bioensaio/métodos , Qualidade de Produtos para o Consumidor , Teste de Materiais/métodos , Testes de Mutagenicidade/métodos , Nanopartículas/toxicidade , Nanotecnologia/métodos , Medição de Risco/métodos , Animais , Humanos
10.
Cells Tissues Organs ; 198(5): 327-37, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24356396

RESUMO

Interactions of human mesenchymal stem cells (hMSC) with tumors are controversially discussed since there is evidence for both tumor progression as well as tumor inhibition by hMSC. The objective of the present study is to investigate whether hMSC support cell motility and cytokine secretion in a head and neck squamous cell carcinoma cell line (HLaC 78). A spheroid model was generated in which the ultrastructure of spheroids was analyzed using scanning electron microscopy (SEM). The migration capability was monitored in a monolayer as well as in a spheroid model. The variation in migration and secretion of interleukin (IL)-6, IL-8 and vascular endothelial growth factor (VEGF), as well as the expression of the multidrug resistance gene (MDR-1) was investigated. Finally, the alteration in the cell cycle was analyzed by flow cytometry. SEM showed a tight cell-cell contact with extensive secretion of extracellular matrix. The migration and invasion capability of HLaC 78 was enhanced by hMSC. Cancer cell motility was also increased by hMSC as well as secretion of the cytokines IL-6, IL-8 and VEGF. hMSC did not induce the expression of MDR-1 in HLaC 78, and there was no alteration in the cell cycle of HLaC 78 after cocultivation with hMSC. Our results confirm the important role of hMSC in cancer biology since both an enhancement of cell motility as well as cytokine secretion could be shown. However, based on these findings and those in the current literature, caution must be applied when using hMSC as a carrier for tumor therapy in cancer treatment.


Assuntos
Carcinoma de Células Escamosas/patologia , Comunicação Celular/fisiologia , Movimento Celular/fisiologia , Citocinas/metabolismo , Neoplasias de Cabeça e Pescoço/patologia , Células-Tronco Mesenquimais/citologia , Carcinoma de Células Escamosas/metabolismo , Linhagem Celular Tumoral , Neoplasias de Cabeça e Pescoço/metabolismo , Humanos , Células-Tronco Mesenquimais/metabolismo , Esferoides Celulares/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço
11.
Cells Tissues Organs ; 197(5): 384-98, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23485626

RESUMO

INTRODUCTION: Adipose tissue-derived stem cells (ASCs) have become the primary focus of tissue engineering research. To understand their functions and behavior in in vitro and in vivo models, it is mandatory to track the implanted cells and distinguish them from the resident or host cells. A common labeling method is the use of fluorescent dyes, e.g. the lipophilic carbocyanine dye, DiI. This study aimed to analyze potential DNA damage, toxicity and impairment of the functional properties of human ASCs after labeling with DiI. METHODS: Cytotoxicity was measured using the MTT assay and DNA damage was determined by means of the comet assay. Potential apoptotic effects were determined using the annexin V-propidium iodide test. Differentiation potential was evaluated by trilineage differentiation procedures in labeled and unlabeled ASCs. Proliferation as well as migration capability was analyzed, and the duration and stability of DiI labeling in ASCs during in vitro expansion was observed over a period of 35 days. RESULTS: DiI labeling did not cause genotoxic effects 15, or 30 min or 24 h after the labeling procedure, and there were no cytotoxic effects until 72 h afterwards. No impairment of proliferation or migration capability or differentiation potential could be determined. However, after 35 days, only 37% of labeled cells could be detected using the fluorescence microscope, which indicates a decrease in staining stability during in vitro expansion. CONCLUSION: DiI is a convenient method for ASCs labeling which causes no toxic effects and does not impair the proliferation, migration or differentiation potential of ASCs after the labeling procedure.


Assuntos
Tecido Adiposo/citologia , Carbocianinas/metabolismo , Carbocianinas/toxicidade , Dano ao DNA , Células-Tronco/citologia , Anexina A5/metabolismo , Morte Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Linhagem da Célula/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Humanos , Fenótipo , Propídio/metabolismo , Coloração e Rotulagem , Células-Tronco/efeitos dos fármacos , Células-Tronco/metabolismo , Azul Tripano/metabolismo
12.
Toxicol Lett ; 218(3): 207-14, 2013 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-23410960

RESUMO

Various hypotheses on the origin of cancer stem cells (CSCs) exist, including that CSCs develop from transformed human bone marrow mesenchymal stem cells (hBMSC). Since the polyether antibiotic salinomycin selectively kills CSCs, the present study aims to elucidate the effects of salinomycin on normal hBMSC. The immunophenotype of hBMSC after salinomycin exposure was observed by flow cytometry. The multi-differentiation capacity of hBMSC was evaluated by Oil Red O and van Kossa staining. Cytotoxic effects of salinomycin were monitored by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay. Furthermore, spheroid formation and migration capacity were assessed. There were no differences in the immunophenotype and multi-differentiation capacity of hBMSC induced by salinomycin treatment. Cytotoxic effects were observed at concentrations of 30 µM and above. Neither the migration capability nor the ability to form spheroids was affected. Essential functional properties of hBMSC were unaffected by salinomycin. However, dose-dependent cytotoxicity effects could be observed. Overall, low dose salinomycin showed no negative effects on hBMSC. Since mesenchymal stem cells from various sources respond differently, further in vitro studies are needed to clarify the effect of salinomycin on tissue-specific stem cells.


Assuntos
Células da Medula Óssea/efeitos dos fármacos , Células-Tronco Mesenquimais/efeitos dos fármacos , Piranos/toxicidade , Células da Medula Óssea/imunologia , Células da Medula Óssea/patologia , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Citometria de Fluxo , Humanos , Imunofenotipagem/métodos , Células-Tronco Mesenquimais/imunologia , Células-Tronco Mesenquimais/patologia
13.
Toxicol Lett ; 207(1): 89-95, 2011 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-21864657

RESUMO

Current pollution limits indicating potential harm to human health caused by nitrogen dioxide have prompted a variety of studies on the cytotoxicity and genotoxicity of nitrogen dioxide (NO2) in vitro. The present study focuses on toxic effects of NO2 at the WHO defined 1-h limit value of 200 µg NO2/m(3) air, equivalent to 0.1 ppm NO2. Nasal epithelial mucosa cells of 10 patients were cultured as an air-liquid interface and exposed to 0.1 ppm NO2 for 0.5 h, 1 h, 2 h and 3 h and synthetic air as negative control. After exposure, analysis of genotoxicity was performed by the alkaline single cell microgel electrophoresis (comet) assay and by the micronucleus test. Depression of proliferation and cytotoxic effects were checked by the micronucleus assay and the trypan blue exclusion assay. The experiments demonstrated significant DNA fragmentation even at the shortest exposure duration of half an hour in the comet assay. The amount of DNA fragmentation significantly increased with extended NO2 exposure durations. The amount of DNA fragmentation increased with extended exposure durations to synthetic air at a significantly lower level as compared to NO2 exposure. Micronucleus inductions were seen only at the longest exposure duration of 3h. There were no changes in proliferation seen in the micronucleus assay under any experimental setup. Moreover, no signs of necrosis, apoptosis or changes in viability were detected. Data demonstrate genotoxicity of NO2 at concentrations found in the urban atmosphere during short exposure durations. DNA alterations in the micronucleus assay at an exposure time of 3h indicate a significant DNA alteration possibly being hazardous to humans.


Assuntos
Fragmentação do DNA/efeitos dos fármacos , Mucosa Nasal/efeitos dos fármacos , Dióxido de Nitrogênio/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Humanos , Testes para Micronúcleos , Mucosa Nasal/citologia , Mucosa Nasal/metabolismo , Estatísticas não Paramétricas
14.
Laryngorhinootologie ; 89(8): 460-4, 2010 Aug.
Artigo em Alemão | MEDLINE | ID: mdl-20714971

RESUMO

Aasthma is one of the most common chronic diseases with a prevalence of 5% in Germany. Nearly half of the patients complain about permanent voice disorders. Mucosal changes due to the obstructive respiratory disease as well as mucus abnormalities and regularly accompanying chronic rhinosinusitis may explain these symptoms. The additional influence of laryngopharyngeal reflux is discussed controversially. Additionally, dysphonia may as well occur due to side effects of the therapy with inhaled corticosteroids: the ingredients as well as physical effects may be responsible for the development of chronic laryngitis. The concomitant therapy by an ENT specialist is important in asthma-related voice disorders to identify the basic cause of dysphonia systematically and to intervene at an early stage.


Assuntos
Asma/diagnóstico , Distúrbios da Voz/diagnóstico , Administração por Inalação , Corticosteroides/administração & dosagem , Corticosteroides/efeitos adversos , Asma/tratamento farmacológico , Asma/fisiopatologia , Disfonia/diagnóstico , Disfonia/fisiopatologia , Humanos , Refluxo Laringofaríngeo/diagnóstico , Refluxo Laringofaríngeo/fisiopatologia , Laringoscopia , Laringe/efeitos dos fármacos , Laringe/fisiopatologia , Muco/efeitos dos fármacos , Muco/fisiologia , Ventilação Pulmonar/efeitos dos fármacos , Ventilação Pulmonar/fisiologia , Fatores de Risco , Prega Vocal/efeitos dos fármacos , Prega Vocal/fisiopatologia , Distúrbios da Voz/induzido quimicamente , Distúrbios da Voz/fisiopatologia
15.
Artigo em Inglês | MEDLINE | ID: mdl-20367549

RESUMO

Numerous manufacturing techniques for autogenous fibrin glue used as scaffold material have been described. As there is no consensus regarding the influence of chemical additives on cell biology, it was the aim of this study to establish a method for manufacturing autologous fibrin glue without any additives. The serum part was separated from whole blood. After fibrinogen precipitation, centrifugation was performed to obtain the fibrinogen pellet. Various experimental series were run to examine influences of various temperatures or substituting centrifugation for sedimentation. The method as described here is effective, simple, and performed without any additives, which could potentially influence cell biology.


Assuntos
Aprotinina/química , Adesivo Tecidual de Fibrina , Fibrinogênio , Engenharia Tecidual/métodos , Tecidos Suporte , Cartilagem/química , Centrifugação , Adesivo Tecidual de Fibrina/química , Fibrinogênio/química , Géis/química , Humanos
16.
Toxicol Appl Pharmacol ; 245(2): 219-25, 2010 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-20214917

RESUMO

Cytotoxicity and genotoxicity of nitrogen dioxide (NO(2)) as part of urban exhaust pollution are widely discussed as potential hazards to human health. This study focuses on toxic effects of NO(2) in realistic environmental concentrations with respect to the current limit values in a human target tissue of volatile xenobiotics, the epithelium of the upper aerodigestive tract. Nasal epithelial cells of 10 patients were cultured as an air-liquid interface and exposed to 0.01 ppm NO(2), 0.1 ppm NO(2), 1 ppm NO(2), 10 ppm NO(2) and synthetic air for half an hour. After exposure, genotoxicity was evaluated by the alkaline single-cell microgel electrophoresis (Comet) assay and by induction of micronuclei in the micronucleus test. Depression of proliferation and cytotoxic effects were determined using the micronucleus assay and trypan blue exclusion assay, respectively. The experiments revealed genotoxic effects by DNA fragmentation starting at 0.01 ppm NO(2) in the Comet assay, but no micronucleus inductions, no changes in proliferation, no signs of necrosis or apoptosis in the micronucleus assay, nor did the trypan blue exclusion assay show any changes in viability. The present data reveal a possible genotoxicity of NO(2) in urban concentrations in a screening test. However, permanent DNA damage as indicated by the induction of micronuclei was not observed. Further research should elucidate the effects of prolonged exposure.


Assuntos
Exposição Ambiental/efeitos adversos , Mucosa Nasal/efeitos dos fármacos , Dióxido de Nitrogênio/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/ultraestrutura , Humanos , Testes de Mutagenicidade
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