Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 174
Filtrar
1.
Ann Diagn Pathol ; 56: 151847, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34742033

RESUMO

Muscle-invasive bladder carcinoma (MIBC) accounts for 25% of newly diagnosed bladder carcinomas (BCs) and presents a high risk of progression and metastasis. This study aimed to identify reliable biomarkers associated with muscle invasion and prognosis to identify potential therapeutic targets for MIBC. Four gene datasets were downloaded from the Gene Expression Omnibus, and the integrated differentially expressed genes (DEGs) were then subjected to gene ontology (GO) terms and pathway enrichment analyses. Correlation analysis between the expression of the top-ranking DEGs and pathological T stages was performed to identify the genes associated with early muscle invasion. The corresponding prognostic values were evaluated, and co-expressed genes mined in the cBioPortal database were loaded into ClueGo in Cytoscape for pathway enrichment analysis. Using data mining from the STRING and TCGA databases, protein-protein interaction and competitive endogenous RNA networks were constructed. In total, 645 integrated DEGs were identified and these were mainly enriched in 26 pathways, including cell cycle, bladder cancer, DNA replication, and PPAR signaling pathway. S100A7 expression was significantly increased from the T2 stage and showed significantly worse overall survival and disease-specific survival in patients with BC. In total, 144 genes co-expressed with S100A7 in BC were significantly enriched in the IL-17 pathway. S100A7 was predicted to directly interact with LYZ, which potentially shows competitive binding with hsa-mir-140 to affect the expression of six lncRNAs in MIBC. In conclusion, high S100A7 expression was predicted to be associated with early muscle invasion and poor survival in patients with BC.

2.
Front Public Health ; 9: 769672, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34760866

RESUMO

This article uses data from the government work reports of 31 provinces (autonomous regions and municipalities) in China and 21 cities in Guangdong Province of China to perform a fixed effect regression. It was found that economic growth target constraints and economic growth target gaps between countries and provinces, or between provinces and cities have a significant impact on the quality of public occupational health. The non-linear relationship between economic growth target setting and the quality of public occupational health was then discussed in detail, and the reliability of basic conclusions drawn was ensured by robustness and endogeneity tests. The results show that the effect of economic growth target constraints and gaps on the quality of public occupational health shows a "U-shaped" trend at both the provincial and city levels, which initially promotes and, eventually, inhibits. This relationship is closely related to the current economic system reforms, administrative reforms, and social transformation in China. Therefore, in emphasising high-quality economic development, the government should fully consider the actual state of the development of jurisdictions in setting economic goals to improve the quality of public occupational health in an orderly manner.


Assuntos
Desenvolvimento Econômico , Saúde do Trabalhador , China , Governo Local , Reprodutibilidade dos Testes
3.
Front Plant Sci ; 12: 734775, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34764966

RESUMO

Although leaf economics spectrum (LES) has been extensively tested with regional and global datasets, the correlation among functional traits of desert plants remains largely unclear. Moreover, examinations on whether and how leaf and root traits are coordinated have yielded mixed results. We investigated variations in leaf and fine-root traits across 48 species in a desert community of northern China to test the hypotheses that (1) the leaf-trait syndrome of plant species in desert shrublands follows the predictions of the global LES, and is paralleled by a similar root-trait syndrome, (2) functional traits related to nutrient contents and resource uptake are tightly coordinated between leaves and fine roots in desert ecosystems where plant growth is limited primarily by dry and nutrient-poor conditions, and (3) traits as well as their relationships vary among functional groups. Our results partially supported the LES theory. Specific leaf area (SLA) was correlated with leaf tissue density, phosphorus content, and carbon-to-nitrogen ratio, but not with leaf nitrogen content. Specific root length (SRL) was not correlated with other fine-root traits, and fine-root traits were largely independent of each other. Therefore, fine-root traits did not mirror the leaf-trait syndrome. Fine-root nitrogen and phosphorus contents, nitrogen-to-phosphorous ratio, and carbon-to-nitrogen ratio all increased with analogous leaf traits, whereas SRL was not correlated with SLA. After phylogenetic effects were considered, nutrient contents and their ratios still displayed stronger coordination between leaves and fine roots than did SRL and SLA. The overall pattern of trait variations and relationships suggested differentiation among functional groups. Our results suggest that despite the absence of a root-trait syndrome, fine-root functions in the studied desert community were probably coordinated with leaf functions with respect to nutrient allocation and use.

4.
ACS Nano ; 15(10): 16664-16672, 2021 10 26.
Artigo em Inglês | MEDLINE | ID: mdl-34636539

RESUMO

Planar, thin-layered chiral plasmonic superstructures with complex two-dimensional (2D) patterns, namely, double-layered binary stars (bi-stars) and pinwheels, were realized through DNA programmable 2D supramolecular self-assembly of gold nanorods (AuNRs). The chirality of the chiral superstructures was defined by a finite number of AuNR pairs as enantiomeric motifs, and their sizes (∼240 nm) were precisely defined by the underlying DNA template. These planar, thin-layered chiral nanoparticle superstructures exhibited prescribed shapes and sizes at the dried state on the substrate surface and are characteristic of giant anisotropy of chiroptical responses, with enhanced g-factors from the axial incident excitation as compared to the in-plane excitation. This work will inspire possibilities for the construction of 2D chiral materials, for example, chiral metasurfaces, for the on-chip manipulation of chiral light-matter interactions via programmable self-assembly of nanoparticles.


Assuntos
Nanopartículas Metálicas , Nanotubos , DNA , Ouro , Estereoisomerismo
5.
Oxid Med Cell Longev ; 2021: 7656834, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616504

RESUMO

Trigonelline is the main alkaloid with bioactivity presented in fenugreek, which was used in traditional medicine in Asian countries for centuries. It is reported that trigonelline has anti-inflammatory, anti-oxidant, and anti-pathogenic effects. We are wondering whether trigonelline have anti-aging effect. We found that 50 µM of trigonelline had the best anti-aging activity and could prolong the lifespan of Caenorhabditis elegans (C. elegans) by about 17.9%. Trigonelline can enhance the oxidative, heat, and pathogenic stress resistance of C. elegans. Trigonelline could also delay the development of neurodegenerative diseases, such as AD, PD, and HD, in models of C. elegans. Trigonelline could not prolong the lifespan of long-lived worms with loss-of-function mutations in genes regulating energy and nutrition, such as clk-1, isp-1, eat-2, and rsks-1. Trigonelline requires daf-16, hsf-1, and aak-2 to extend the lifespan of C. elegans. Trigonelline can also up-regulate the expression of daf-16 and hsf-1 targeted downstream genes, such as sod-3, gst-4, hsp-16.1, and hsp-12.6. Our results can be the basis for developing trigonelline-rich products with health benefits, as well as for further research on the pharmacological usage of trigonelline.

6.
BMC Cancer ; 21(1): 1135, 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34688260

RESUMO

BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is one of the most lethal urological malignancies, but the pathogenesis and prognosis of ccRCC remain obscure, which need to be better understand. METHODS: Differentially expressed genes were identified and function enrichment analyses were performed using three publicly available ccRCC gene expression profiles downloaded from the Gene Expression Omnibus database. The protein-protein interaction and the competing endogenous RNA (ceRNA) networks were visualized by Cytoscape. Multivariate Cox analysis was used to predict an optimal risk mode, and the survival analysis was performed with the Kaplan-Meier curve and log-rank test. Protein expression data were downloaded from Clinical Proteomic Tumor Analysis Consortium database and Human Protein Atlas database, and the clinical information as well as the corresponding lncRNA and miRNA expression data were obtained via The Cancer Genome Atlas database. The co-expressed genes and potential function of candidate genes were explored using data exacted from the Cancer Cell Line Encyclopedia database. RESULTS: Of the 1044 differentially expressed genes shared across the three datasets, 461 were upregulated, and 583 were downregulated, which significantly enriched in multiple immunoregulatory-related biological process and tumor-associated pathways, such as HIF-1, PI3K-AKT, P53 and Rap1 signaling pathways. In the most significant module, 36 hub genes were identified and were predominantly enriched in inflammatory response and immune and biotic stimulus pathways. Survival analysis and validation of the hub genes at the mRNA and protein expression levels suggested that these genes, particularly complement component 3 (C3) and fibronectin 1 (FN1), were primarily responsible for ccRCC tumorigenesis and progression. Increased expression of C3 or FN1 was also associated with advanced clinical stage, high pathological grade, and poor survival in patients with ccRCC. Univariate and multivariate Cox regression analysis qualified the expression levels of the two genes as candidate biomarkers for predicting poor survival. FN1 was potentially regulated by miR-429, miR-216b and miR-217, and constructed a bridge to C3 and C3AR1 in the ceRNA network, indicating a critical position of FN1. CONCLUSIONS: The biomarkers C3 and FN1 could provide theoretical support for the development of a novel prognostic tool to advance ccRCC diagnosis and targeted therapy.

7.
Artigo em Inglês | MEDLINE | ID: mdl-34705204

RESUMO

In this study, the groundwater (GW) in the high-fluorine area of the Southwestern Shandong Plain was divided according to the characteristics of high Na% (> 75%), and its water chemistry characteristics and causes were discussed separately, and the hydrochemical process of the formation of high-fluorine GW was determined. Finally, the GW quality of the study area was evaluated. The results proved that silicate hydrolysis can significantly promote the release of F- in fluorine-containing minerals; high %Na can be used as one of the early-warning conditions for judging high-fluoride areas. To this end, 132 GW samples were collected from 66 wells during the dry and wet seasons. The study area was found to have weakly alkaline GW (pH 7.1-8.9) and could be divided into high %Na areas (HNA) and non-HNA. GW exhibited different hydrochemical characteristics between HNA and non-HNA. In non-HNA, total hardness (TH) exceeded 200 mg/L, and total dissolved solids (TDS) ranged from 514.1 to 5246.1 mg/L; in HNA, TH was less than 200 mg/L, TDS ranged from 552.8 to 1298.3 mg/L, and Na+ increased with TDS, whereas Ca2+ and Mg2+ contents were low. The main water type in HNA was HCO3-Na and in non-HNA was SO4·Cl-Ca·Mg and SO4·Cl-Na. The study area is experiencing serious fluoride pollution. GW in HNA is mostly controlled by carbonate and silicate hydrolysis and evaporation, whereas GW in non-HNA is controlled by dolomite dissolution and cation exchange in the main. Moreover, GW in HNA has significantly been altered by albite hydrolysis, which produces Na+ and HCO3- and triggers various reactions promoting the release of F- from fluorine-containing minerals (FCM). Regarding the water quality for irrigation, GW in HNA was found to be less suitable than that in non-HNA. Nevertheless, in terms of the water quality index (WQI), GW is moderate for drinking and poor for irrigation. Therefore, extensive attention should be paid to the exploitation and management of high-sodium GW in the plain area.

8.
J Stroke Cerebrovasc Dis ; 30(11): 106071, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34482123

RESUMO

OBJECTIVE: To validate, update, and extend the role of RNF213 p.R4810K (G>A) for predicting the phenotype of moyamoya disease (MMD) patients and explore the different effects on pediatric and adult groups. METHODS: A total of 2,877 patients conducted from 2004 to 2018 were included. Review Manage 5.3 and SPSS 20.0 were applied to complete all statistical analyses. Information on age at onset, sex, initial symptom, family history and complications were obtained via retrospective chart review. Angiographic records were evaluated. RESULTS: In China, geographic proximity to Korea or Japan may affect the carrying rate of RNF213 p.R4810K. The proportion of patients with the following characteristics was significantly higher (P <0.017) in the GA than in the GG group: female, age at onset < 18 years, infarct after transient ischemic attack, family history of MMD, and posterior cerebral artery involvement. For pediatric patients, GA showed more cerebral hemorrhage (CH) (odds ratios (ORs) [95% confidence intervals (CIs)] = 3.99 (1.61-9.88), P = 0.003), more patients were in the Suzuki early and intermediate stage (P = 0.001; P = 0.001, respectively), while for the adult group, GA indicated more female (OR [95% CIs] = 1.43 [1.15-1.79], P = 0.001), fewer patients with diabetes (0.58 [0.38-0.86], P = 0.007) and intermediate Suzuki stage (P = 3.70 × 10-4). CONCLUSIONS: The incidence and carrying rates of RNF213 p.R4810K in various regions for Chinese MMD patients were obviously different. RNF213 p.R4810K has different predictive effects on phenotypes of pediatric and adult patients.

9.
PLoS Pathog ; 17(9): e1009940, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34543359

RESUMO

Viruses use diverse strategies to impair the antiviral immunity of host in order to promote infection and pathogenesis. Herein, we found that PCV2 infection promotes the infection of DNA viruses through inhibiting IFN-ß induction in vivo and in vitro. In the early phase of infection, PCV2 promotes the phosphorylation of cGAS at S278 via activation of PI3K/Akt signaling, which directly silences the catalytic activity of cGAS. Subsequently, phosphorylation of cGAS at S278 can facilitate the K48-linked poly-ubiquitination of cGAS at K389, which can been served as a signal for recognizing by the ubiquitin-binding domain of histone deacetylase 6 (HDAC6), to promote the translocation of K48-ubiquitinated-cGAS from cytosol to autolysosome depending on the deacetylase activity of HDAC6, thereby eventually resulting in a markedly increased cGAS degradation in PCV2 infection-induced autophagic cells relative to Earle's Balanced Salt Solution (EBSS)-induced autophagic cells (a typical starving autophagy). Importantly, we found that PCV2 Cap and its binding protein gC1qR act as predominant regulators to promote porcine cGAS phosphorylation and HDAC6 activation through mediating PI3K/AKT signaling and PKCδ signaling activation. Based on this finding, gC1qR-binding activity deficient PCV2 mutant (PCV2RmA) indeed shows a weakened inhibitory effect on IFN-ß induction and a weaker boost effect for other DNA viruses infection compared to wild-type PCV2. Collectively, our findings illuminate a systematic regulation mechanism by which porcine circovirus counteracts the cGAS-STING signaling pathway to inhibit the type I interferon induction and promote DNA virus infection, and identify gC1qR as an important regulator for the immunosuppression induced by PCV2.

11.
Front Oncol ; 11: 685980, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34249735

RESUMO

Bladder urothelial carcinoma (BC) is a fatal invasive malignancy and the most common malignancy of the urinary system. In the current study, we investigated the function and mechanisms of Neuropilin-1 (NRP1), the co-receptor for vascular endothelial growth factor, in BC pathogenesis and progression. The expression of NRP1 was evaluated using data extracted from GEO and HPA databases and examined in BC cell lines. The effect on proliferation, apoptosis, angiogenesis, migration, and invasion of BC cells were validated after NRP1 knockdown. After identifying differentially expressed genes (DEGs) induced by NRP1 silencing, GO/KEGG and IPA® bioinformatics analyses were performed and specific predicted pathways and targets were confirmed in vitro. Additionally, the co-expressed genes and ceRNA network were predicted using data downloaded from CCLE and TCGA databases, respectively. High expression of NRP1 was observed in BC tissues and cells. NRP1 knockdown promoted apoptosis and suppressed proliferation, angiogenesis, migration, and invasion of BC cells. Additionally, after NRP1 silencing the activity of MAPK signaling and molecular mechanisms of cancer pathways were predicted by KEGG and IPA® pathway analysis and validated using western blot in BC cells. NRP1 knockdown also affected various biological functions, including antiviral response, immune response, cell cycle, proliferation and migration of cells, and neovascularisation. Furthermore, the main upstream molecule of the DEGs induced by NRP1 knockdown may be NUPR1, and NRP1 was also the downstream target of NUPR1 and essential for regulation of FOXP3 expression to activate neovascularisation. DCBLD2 was positively regulated by NRP1, and PPAR signaling was significantly associated with low NRP1 expression. We also found that NRP1 was a predicted target of miR-204, miR-143, miR-145, and miR-195 in BC development. Our data provide evidence for the biological function and molecular aetiology of NRP1 in BC and for the first time demonstrated an association between NRP1 and NUPR1, FOXP3, and DCBLD2. Specifically, downregulation of NRP1 contributes to BC progression, which is associated with activation of MAPK signaling and molecular mechanisms involved in cancer pathways. Therefore, NRP1 may serve as a target for new therapeutic strategies to treat BC and other cancers.

12.
Ying Yong Sheng Tai Xue Bao ; 32(7): 2407-2414, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34313058

RESUMO

Evapotranspiration (ET) is an important part of water cycle and energy flow in ecosystem. Accurate estimation of ET and its components is critical for understanding the impacts of ecophysiological processes on ecosystem water balance and plant water use strategy. Using the eddy-covariance technique and the micro-lysimeter, we measured ET, evaporation (E), transpiration (T) of the Artemisia ordosica-Hedysarum fruticosum var. mongolicum shrubland in the Mu Us Desert during May 20 to September 15, 2019, quantified the ET components, and analyzed the seasonal characteristics and influencing factors of ET and its components. The results showed that T was the main component of ET in the growing season, with a T/ET of 53.1%. T/ET increased and E/ET decreased as precipitation decreased. The partitioning of evapotranspiration was regulated by precipi-tation. At the seasonal scale, the value of E was positively correlated with soil water content at 10 cm depth (SWC10) and net radiation (Rn), while SWC10 was the main factor influencing E. The value of T increased with the increases of Rn and leaf area index (LAI), and increased first and then decreased with the increases of soil water content at 30 cm layer (SWC30). T was affected by SWC30, Rn and LAI. Moisture was the main influencing factor of ET. The ET/P in the growing season was 109.2% and was 250.5% in May, indicating that the water consumption of ET in early growing season was partly from the precipitation in non-growing season.


Assuntos
Artemisia , Ecossistema , China , Transpiração Vegetal , Estações do Ano , Solo , Água
13.
J Int Med Res ; 49(7): 3000605211019879, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34308690

RESUMO

OBJECTIVE: To explore the significance of the prostate central gland to total gland volume ratio (PVc/PV) in the diagnosis of prostate cancer (PCa) in patients with prostate specific antigen (PSA) levels in the grey zone (4-10 ng/ml). METHODS: This retrospective study enrolled patients that had undergone prostate biopsy. The volume of the prostate and the central prostate gland were measured. The differences in PSA, the ratio of free to total PSA (f/tPSA), PSA density (PSAD) and PVc/PV between the PCa and non-PCa groups were compared. Receiver operating characteristic curve analysis for PCa and clinically significant PCa (csPCa) diagnosis were calculated according to PSA (reference), f/tPSA, PSAD and PVc/PV. RESULTS: This study enrolled 136 patients. There was no significant difference in PSA and f/tPSA between the PCa and non-PCa groups, while there were significant differences in PSAD and PVc/PV. The area under the curve values of PVc/PV for PCa or csPCa diagnosis were 0.876 and 0.933, respectively; and for PSAD, they were 0.705 and 0.790, respectively. These were significantly different compared with the PSA curve, whereas f/tPSA showed no significant difference from the PSA curve. CONCLUSION: PVc/PV could be a predictor of PCa when PSA is between 4-10 ng/ml.


Assuntos
Antígeno Prostático Específico , Neoplasias da Próstata , Biópsia , Humanos , Masculino , Neoplasias da Próstata/diagnóstico , Curva ROC , Estudos Retrospectivos
14.
Carbohydr Polym ; 267: 118170, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34119142

RESUMO

The lack of gluten in Tartary buckwheat has always been the main limiting factor of their development. This paper explored how to improve the processing quality of gluten-free Tartary buckwheat noodles (GF-TBNs) by introducing extruded starch into Tartary buckwheat flour (TBF) and the underlying mechanism was also elucidated. Extruded Tartary buckwheat starch (ETBS) was obtained under different extrusion conditions. The thermal properties, molecular weight, and viscosity of ETBS were examined to determine the key parameters closely related to the water distribution and rheological properties of the dough sheet, and tensile properties of GF-TBNs. The results showed that ETBS with a low molecular weight and high viscosity contributed greatly to the GF-TBNs with good tensile properties. It is proposed that ETBS with a low molecular weight and high viscosity might form a gel-entrapped network inside GF-TBNs, which was confirmed by the morphology of GF-TBNs.


Assuntos
Grão Comestível/química , Amido/química , Elasticidade , Fagopyrum/química , Farinha , Manipulação de Alimentos/métodos , Proteínas de Plantas/química , Temperatura , Resistência à Tração , Viscosidade
15.
Environ Pollut ; 285: 117482, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34082370

RESUMO

This study collected 184 groundwater (GW) samples from 92 wells during the dry and wet seasons, respectively to understand the hydrochemical characteristics, water quality, and risk of GW nitrate (NO3-) to human health in northern Shandong Peninsula (NSP), China. The results showed that GW in the NSP is weakly alkaline and classified as hard fresh water. The mean concentration of NO3- in GW exceeded 100 mg·L-1, total hardness exceeded 450 mg·L-1, and total dissolved solids (TDS) was less than 1000 mg·L-1. A Piper diagram showed that the water chemistry of GW in the NSP was mainly of the SO4·Cl-Ca·Mg type. A Gibbs diagram and ion ratio analysis indicated that the weathering of silicate rocks and agricultural production were the dominant factors affecting the hydrochemical characteristics of GW in the NSP, with cation exchange, dissolution of salt rock, and weathering of carbonate rock also making contributions. Na+ and Cl- in GW are significantly affected by seawater aerosols in coastal areas. During the wet season, the hydrodynamic conditions of the aquifer are improved, agricultural activities are strengthened, and GW becomes generally homogenized. The water quality index classified the GW quality of the NSP as mainly of medium quality. There was a relatively consistent spatial distribution of water quality during different periods. Water samples of poor water quality were mainly distributed in the lower reaches of the Huangshui River. In addition, total hardness and NO3- concentrations were the main variables affecting the quality of GW in the NSP. The assessment of the risk NO3- in GW in the NSP to human health through the ingestion of drinking water demonstrated a significant risk (infants > children > adults). These results indicate the need for local management measures to reduce the potential health risks of GW quality in the NSP.


Assuntos
Água Subterrânea , Poluentes Químicos da Água , Adulto , Criança , China , Monitoramento Ambiental , Humanos , Lactente , Poluentes Químicos da Água/análise , Qualidade da Água
16.
Artigo em Inglês | MEDLINE | ID: mdl-34129138

RESUMO

In this study, the formation mechanism and water quality of groundwater in the northwest of Nansi Lake Catchment (NNLC) were analyzed through mathematical statistics, hydrochemical analysis and entropy weighted water quality index (EWQI), and the human health risk of nitrate was also evaluated. To this end, 89 wells in the NNLC were sampled, and the groundwater samples were divided into three groups (I, II, and III) according to cluster analysis results and spatial distribution. The main results are as follows: Topographically, Groups I, II, and III correspond to the alluvial plains, apron plain, and low hills and its front margin, respectively. According to the Piper diagram, the hydrochemical types of Groups I and II groundwater are Na-SO4·Cl and Ca·Mg-HCO3, respectively, and that of Group III is more concentrated, mostly corresponding to the Ca-HCO3 type. Hydrochemical analysis indicated that the development of groundwater hydrochemistry is mainly attributable to water-rock interactions, with the primary process being the dissolution of minerals such as calcite, dolomite, gypsum, and albite. Evaporation exhibited an increasing trend from the northeast to the southwest. Groups I and III presented obvious effects of human activities, with Group I showing sulfate pollution and Group III mainly showing nitrate pollution. Analysis of the characteristics and causes of the groundwater hydrochemistry revealed the proposed approach has excellent performance for classification in areas with complex hydrogeological conditions. The results of EWQI showed that the overall water quality was good, following the order Group III > Group II > Group I. The overall human health risk of nitrate in groundwater was low, but the risk was slightly higher for children than for adults. Therefore, the effects of nitrate contamination should be considered when exploiting hilly and peri-urban groundwater for drinking water.

17.
Cell Death Dis ; 12(5): 442, 2021 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-33947842

RESUMO

Despite N6-methyladenosine (m6A) is functionally important in various biological processes, its role and the underlying regulatory mechanism in the liver remain largely unexplored. In the present study, we showed that fat mass and obesity-associated protein (FTO, an m6A demethylase) was involved in mitochondrial function during hepatic ischemia-reperfusion injury (HIRI). We found that the expression of m6A demethylase FTO was decreased during HIRI. In contrast, the level of m6A methylated RNA was enhanced. Adeno-associated virus-mediated liver-specific overexpression of FTO (AAV8-TBG-FTO) ameliorated the HIRI, repressed the elevated level of m6A methylated RNA, and alleviated liver oxidative stress and mitochondrial fragmentation in vivo and in vitro. Moreover, dynamin-related protein 1 (Drp1) was a downstream target of FTO in the progression of HIRI. FTO contributed to the hepatic protective effect via demethylating the mRNA of Drp1 and impairing the Drp1-mediated mitochondrial fragmentation. Collectively, our findings demonstrated the functional importance of FTO-dependent hepatic m6A methylation during HIRI and provided valuable insights into the therapeutic mechanisms of FTO.


Assuntos
Adenosina/análogos & derivados , Dioxigenase FTO Dependente de alfa-Cetoglutarato/metabolismo , Dinaminas/metabolismo , Fígado/irrigação sanguínea , Mitocôndrias Hepáticas/metabolismo , Traumatismo por Reperfusão/metabolismo , Adenosina/metabolismo , Animais , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias Hepáticas/patologia , Traumatismo por Reperfusão/patologia
18.
J Neurol ; 268(12): 4768-4777, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33956201

RESUMO

BACKGROUND AND PURPOSE: This study aimed to investigate the enhancement characteristics of vessel wall in patients with moyamoya disease (MMD) using 3D high-resolution magnetic resonance (MR) imaging and their relationship with initial and recurrent intracranial hemorrhage. METHODS: Consecutive patients with MMD were retrospectively analyzed and classified as intracranial hemorrhagic and non-hemorrhagic groups according to the CT or MR images. The clinical features and vessel wall characteristics were compared between the two groups. Logistic regression was performed to relate the vessel wall characteristics to the initial hemorrhage in MMD patients. Patients in hemorrhagic group were followed up after surgery to evaluate the relationship between vessel wall characteristics and recurrent hemorrhage. RESULTS: A total of 507 MMD patients including 79 hemorrhagic and 428 non-hemorrhagic MMD patients were recruited in the study. We found that hemorrhagic group had more patients with vessel wall enhancements (40.5% vs. 25.7%, p = 0.009) and more eccentric enhanced lesions (17.7% vs. 6.5%, p = 0.001) compared to those in non-hemorrhage group and vessel wall enhancements were independently associated with ipsilateral initial hemorrhage after adjusted for clinical factors (OR = 1.99, CI 1.20-3.28, p = 0.007). Furthermore, three recurrent intracranial hemorrhagic episodes in the present study were all observed in MMD patients with vessel wall enhancement during the long-term follow-up after surgery. CONCLUSIONS: Wall enhancement of intracranial vessels was significantly associated with intracranial hemorrhage in MMD patients. Our findings suggest that vessel wall enhancement may serve as a marker of intracranial hemorrhage.


Assuntos
Doença de Moyamoya , Hemorragia Cerebral , Humanos , Hemorragias Intracranianas , Imageamento por Ressonância Magnética , Doença de Moyamoya/complicações , Doença de Moyamoya/diagnóstico por imagem , Estudos Retrospectivos
19.
Vet Microbiol ; 258: 109098, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33984793

RESUMO

Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection via immunosuppression. Although the co-infection of PCV2 and porcine parvovirus (PPV) is commonly observed in worldwide, the relative immune mechanisms promoting PPV infection in PCV2-infected piglets are currently unknown. Herein, we found that PCV2 infection suppressed IFN-ß expression and promoted PPV infection in the piglets. Consistent with this finding, we confirmed that PCV2 infection significantly inhibited the induction of IFN-ß to promote PPV replication in cell level. Furthermore, PCV2 infection attenuated the K63-linked ubiquitination of STING induced by PPV, blocked the formation of complex of STING, TBK1 and IRF3, and further prevented the phosphorylation of TBK1 and IRF3, resulting in a decreased IFN-ß transcription response to PPV infection. Consistently, using cGAMP to direct stimulate STING also appeared a reduced STING-K63 ubiquitination and IFN-ß induction in PCV2-infected cells. However, we noted that knockdown of p38-MAPK signaling could markedly attenuate the inhibitory effect of PCV2 on STING-K63 ubiquitination, and improve the induction of IFN-ß in PCV2-infected whenever theses cells were challenged with PPV infection or cGAMP stimulation. Meanwhile, we found that PCV2 infection promoted the phosphorylation of USP21 to inhibit the K63 ubiquitination of STING and the transcription of IFN-ß via activation of p38-MAPK signaling. Taken together, our results demonstrate that PCV2 infection activates the p38-MAPK signaling pathway-mediated USP21 phosphorylation to inhibit the K63 ubiquitination of STING, which prevents the phosphorylation and transportation to the nucleus of IRF3, leading to an increase risk for PPV infection.


Assuntos
Circovirus/fisiologia , Fator Regulador 3 de Interferon/metabolismo , Interferon beta/metabolismo , Sistema de Sinalização das MAP Quinases/fisiologia , Proteínas de Membrana/metabolismo , Doenças dos Suínos/virologia , Animais , Linhagem Celular , Regulação da Expressão Gênica/imunologia , Fator Regulador 3 de Interferon/genética , Sistema de Sinalização das MAP Quinases/genética , Masculino , Proteínas de Membrana/genética , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Suínos , Testículo/citologia , Ubiquitinação
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 260: 119951, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34049007

RESUMO

GdNbTiO6: Sm3+ phosphors with various Sm3+ concentrations were prepared via a high temperature solid-state reaction method. The crystal structure of the samples was characterized by means of X-ray diffraction (XRD) and the as-prepared samples were confirmed to be orthorhombic phase GdNbTiO6. Photoluminescence properties were investigated by measuring the concentration- and temperature-dependent photoluminescence spectra. Concentration-dependent luminescence quenching and luminescent thermal quenching behaviors were observed and they were respectively ascribed to the electric dipole-dipole interaction between Sm3+ ions and the cooperation of energy transfer and crossover process. The chromatic characteristics were found to be dependent on the excitation wavelength and Sm3+ concentration. In addition, temperature-induced redshift of charge transfer band of GdNbTiO6 host was found in temperature-dependent excitation spectra and the opposite variations of different excitation peaks were utilized for optical thermometry. Finally, the optical transition property was studied on the basis of the diffuse reflectance spectra and Judd-Ofelt (J-O) theory, meanwhile, its accuracy was evaluated by the result of emission spectra.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...