Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 573
Filtrar
1.
Bioresour Technol ; 307: 123204, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32224426

RESUMO

The performance of anaerobic digestion (AD) can be improved by the addition of accelerants. Three types of biomass-derived carbon-based composites (Co/C, CoO/C, and Co3O4/C) were used as accelerants to investigate the effect on AD systems in this work. These accelerants significantly improved the cumulative biogas yield (576-585 mL/g VS), and the total chemical oxygen demand degradation rate (68.48-71.11%) compared to the reference group (435.8 mL/g VS, 50.74%). The digestates with accelerants exhibited exceptional stability (59.24-63.67%) and superior fertilizer utilization (3.50-4.55%). In addition, first-principle density functional theory (DFT) calculations were conducted to provide the theoretical basis for the direct interspecies electron transfer (DIET), and a general strategy was proposed to help understand the enhanced methanogenesis pathway induced by the biomass-derived carbon-based composites. These important findings provide a novel avenue for the development of composite accelerants for AD systems.

2.
Bioengineered ; 11(1): 301-310, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32129699

RESUMO

The influence of freezing-thawing (F/T) pretreatment on the degradation of highly concentrated organic matters from dewatered sludge (DS) in microbial electrolysis cell (MEC) was investigated in this study. Extended freezing disintegrated the DS matrix and resulted in accelerated hydrolysis rate. The biogas production and stabilization were increased due to the pretreatment by 25-70% of H2 production rate and 17.8-33.8% of COD reduction rate, respectively. Fourier transform infrared spectroscopy analysis indicated that the pretreatment was unable to alter the bioelectrochemical reactions except for accelerating degradation rate. Excitation and emission matrix (EEM) spectra showed that aromatic protein and soluble microbial products (SMPs)-like materials in DS were increasingly solubilized by the pretreatment and significantly removed during electrogenesis. The F/T-pretreated DS favored the enrichment of exoelectrogens in MEC.

3.
Int Immunopharmacol ; 82: 106259, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32143000

RESUMO

Proteasome inhibitor bortezomib offers one more option for acute or chronic antibody-mediated rejection after kidney transplantation, but aggravated acute kidney injury (AKI) in some cases early after surgery using bortezomib bring new problem. Here, we evaluated the effects of bortezomib and ONX-0914 on renal tubule injury in a mouse model of ischemia-reperfusion injury. After treated with bortezomib, serum creatinine, usea nitrogen and tubular necrosis significantly increased compared with vehicle-treated mice, but decreased in ONX-0914 group mildly. Infiltration of neutrophil and macrophage were less in bortezomib and ONX-0914-treated mice than vehicle-treated group, and the same was observed on oxidative stress in the kidneys. Furthermore, the apoptosis of renal tubular epithelial cells increased in bortezomib-treated mice' kidneys compared with ONX-0914 and vehicle-treated controls. In vitro HK2 cell experiments also demonstrated the proapoptotic effect of bortezomib. The mRNA expression of several proapoptotic factors increased in kidneys of bortezomib-treated mice. In brief, bortezomib, as a proteasome inhibitor, shows a certain cytotoxicity to renal tubular epithelial cell during ischemia/reperfusion injury (IRI) through increased apoptosis. ONX-0914, as an immunoproteasome inhibitor, showed equal potency on anti-inflammation and oxidative stress relieving compared with bortezomib, while less cytotoxicity. The results render the immunoproteasome is a better target for anti-rejection and protecting kidney function in the field of organ transplantation.

4.
BMC Pregnancy Childbirth ; 20(1): 82, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32033597

RESUMO

BACKGROUND: Water requirements increases with gestational age. Insufficient water intake causes dehydration, which may adversely affect maternal health and birth outcomes. However, few related studies have been conducted. The purposes are to assess the water intake and hydration state among pregnant women, and to investigate the associations with pregnancy complications and maternal and infant outcomes. METHODS: A prospective observational cohort study will be applied. A total of 380 pregnant women will be recruited from the First Affiliated Hospital of Hainan Medical University. Hydration biomarkers and health outcomes will be tested during 15~17 weeks' gestation, 20~22 weeks' gestation, 30~32 weeks' gestation, during childbirth and 42 days after childbirth. Daily fluid intake will be collected using a 24-h fluid intake record for 7 consecutive days. A semi-quantified food frequency method will be used to assess food intake and water intake from food. Anthropometric measurement will be taken following standardized processes. Intracellular fluid (ICF) and extracellular fluid (ECF) will be measured using a body composition analyzer. Morning fasting urine and blood osmolality will be tested by laboratory physicians using an osmotic pressure molar concentration meter. Pregnancy complications will be assessed and diagnosed throughout pregnancy and childbirth. Maternal-infant outcomes will be monitored using related indicators and technologies. In order to explore the internal mechanism and interactions from the perspective of endocrine, pregnancy related hormones (estradiol, prolactin, progesterone) and the hydration-related hormones (copeptin) will be tested during pregnancy. A mixed model of repeated measures ANOVA will be analyzed using SAS 9.2. RESULTS: The results may provide basic data on water intake among pregnant women. The association between hydration state and maternal-infant outcomes will also be explored. CONCLUSIONS: This preliminary exploratory study findings will fill the gaps in the research on water intake, hydration and maternal health, birth outcomes, provide scientific reference data for updating recommendation on water adequate intake among pregnant women, and provide suggestion for developing water intake interventions. TRIAL REGISTRATION: The protocol has been registered on the website of Chinese Clinical Trial Registry. The Identifier code is ChiCTR1800019284. The Registry date is 3 November, 2018. Registry name is "Study for the correlation between hydration state and pregnancy complications, maternal and infant outcomes during pregnancy".

5.
Compr Psychiatry ; 98: 152168, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32105909

RESUMO

BACKGROUND: Insomnia is common during pregnancy but the prevalence and risk factors of insomnia in Chinese women during pregnancy is not well studied. This study aimed to examine the prevalence of insomnia and its risk factors in Chinese women during pregnancy. METHODS: In this cross sectional study, 436 Chinese pregnant women with Insomnia Severity Index (ISI) ≥ 8 were clinically assessed using the insomnia criteria based on the combination of DSM-IV (Diagnostic and Statistical Manual-4th Edition) and ICD-10 (International Classification of Dieases, 10th Edition). Beck Depression Inventory (BDI), State-Trait Anxiety Inventory (STAI), Dysfunctional Beliefs and Attitudes about Sleep Scale (DBAS), Pregnancy Pressure Scale (PPS), Perceived physical discomfort level and number, Epworth Sleepiness Scale (ESS), and a general socio-demographic questionnaire were administered. RESULTS: The results showed that about 20% of the pregnant women met the strict diagnosis criteria of insomnia. Independent-samples t-test revealed that several risk factors were correlated with the group with insomnia (N = 84) compared to the group without insomnia (N = 352). Binary Logistic regression analysis found that more significant bed partner influence (OR = 1.92, 95% CI: 1.03-3.60), depressive symptoms (OR = 1.07, 95% CI: 1.00-1.14), daytime sleepiness (OR = 1.07, 95% CI: 1.01-1.14), subjective somatic discomfort (OR = 2.27, 95% CI: 1.11-4.65), kinds of somatic discomfort (OR = 1.14, 95% CI: 1.03-1.27) and later gestation (OR = 1.05, 95% CI: 1.01-1.09) were significantly associated with insomnia. CONCLUSION: In this cohort of Chinese pregnant women, about a fifth of women suffered from clinically significant insomnia. Measures to prevent the adverse effects of insomnia should be provided to pregnant women with depressive symptoms, Sleep disturbance of the bed partner, excessive daytime sleepiness and somatic discomfort, especially late in gestation.

6.
Sci Total Environ ; 713: 136451, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32019010

RESUMO

Antimony (Sb) and arsenic (As) are toxic elements that occur widely in trace soil concentrations. Expansion of mining activities has increased Sb and As pollution, thus posing a severe threat to human welfare and ecological systems worldwide. Knowledge regarding the composition and adaptation of the microbial communities in these metal(loid) contaminated sites is still limited. In the current study, samples along a river flowing through the world's largest Sb mining area (Xikuangshan) were selected to investigate the microbial response to different Sb or As species. A comprehensive analysis of geochemical parameters, high-throughput sequencing, and statistical methods were applied to reveal the different effects of Sb and As on sedimentary microorganisms. Results suggested that the majority of the Sb and As fractions were not bioavailable. The Sb extractable fraction had a stronger effect on the microbial community compared with its As counterpart. Random forest analyses indicated that the easily exchangeable Sb fraction and specifically sorbed surface-bound fraction were the two most selective variables shaping microbial community diversity. A total of 11 potential keystone phyla, such as bacteria associated with the Bacteroidetes, Proteobacteria, and Firmicutes, were identified according to a molecular ecological network analysis. Strong correlations (|R| > 0.7, P < 0.05) were identified among the indigenous microbial community and pH (negative), sulfate (negative), and exchangeable Sb fraction (positive). Bacteria associated with the genera Geobacter, Phormidium, Ignavibacterium, Desulfobulbus, Ferruginibacter, Fluviicola, Methylotenera, and Scytonema, were predicted to tolerate or metabolize the Sb extractable fraction.


Assuntos
Microbiota , Antimônio , Arsênico , Monitoramento Ambiental , Rios , Poluentes do Solo
7.
Bioorg Chem ; 96: 103650, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32044515

RESUMO

In the current study, four pairs of new enantiomeric alkaloids (1a/1b-4a/4b) were obtained from the leaves of Isatis indigotica Fortune Ex Land. Their structures were elucidated through spectroscopic methods and quantum mechanical calculations. Biologically, all isolates were evaluated for their neuroprotective effects against H2O2-induced SH-SY5Y cell injury. As a result, 1a and 1b exhibited enantioselective neuroprotective effects, further Annexin V-FITC/PI analysis showed that apoptosis ratios of 1a and 1b were reduced to 20.93% and 17.87%, respectively.

8.
Artigo em Inglês | MEDLINE | ID: mdl-32087966

RESUMO

Lipin1 is important in lipid synthesis because of its phosphatidate phosphatase activity, and it also functions as transcriptional coactivators to regulate the expression of genes involved in lipid metabolism. We found that fld mice exhibit cognitive impairment, and it is related to the DAG-PKD-ERK pathway. We used fld mice to explore the relationship between lipin1 and cognitive function. Our results confirmed the presence of cognitive impairment in the hippocampus of lipin1-deficient mice. As shown in behavioral test, the spatial learning and memory ability of fld mice was much worse than that of wild-type mice. Electron microscopy results showed that the number of synapses in hippocampus of fld mice was significantly reduced. BDNF,SYP, PSD95 were significantly reduced. These results suggest that lipin1 impairs synaptic plasticity. Hence,a deficiency of lipin1 leads to decreased DAG levels and inhibits PKD activation, thereby affecting the phosphorylation of ERK and the CREB.

9.
Acta Biochim Biophys Sin (Shanghai) ; 52(2): 125-132, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-31951250

RESUMO

Cordycepin (3'-deoxyadenosine) is a naturally occurring adenosine analog and one of the bioactive constituents isolated from Cordyceps sinensis, species of the fungal genus Cordyceps. It has traditionally been a prized Chinese folk medicine for the human well-being. However, the actions of cordycepin against renal ischemia/reperfusion injury (I/R) are still unknown. In the present study, rats were subject to I/R and cordycepin was intragastrically administered for seven consecutive days before surgery to investigate the effects and mechanisms of cordycepin against renal I/R injury. The test results of kidney and peripheral blood samples of experimental animals showed that cordycepin significantly decreased serum blood urea nitrogen and creatinine levels and markedly attenuated cell injury. Mechanistic studies showed that cordycepin significantly regulated inflammation, apoptosis, and oxidative stress. These data provide new insights for investigating the natural product with the nephroprotective effect against I/R, which should be developed as a new therapeutic agent for the treatment of I/R in the future.

10.
Bioresour Technol ; 301: 122753, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31982852

RESUMO

Phosphorus content of the digestate is crucial for evaluating its fertilizer utilization in anaerobic digestion system. The vermiculite containing rich-phosphorus is firstly used as an accelerant in anaerobic batch co-digestion system of aloe peel waste and dairy manure. After introducing vermiculite, the cumulative biogas production (295.14-353.96 mL/g VS), chemical oxygen demand removal rate (45.53%-71.03%), and volatile solid removal rate (50.70%-52.76%) are remarkably higher than those of reference reactor (234.08 mL/g VS, 39.38%, 45.10%). The thermal and fertility analyses manifest the digestates with vermiculite possess superior stability, admirable fertilizer values (5.97%-6.81%), and excellent total phosphorus content (11.44-13.29 g/kg). The improved co-digestion performance can be attributed to the addition of vermiculite. This work introduces a novel approach for improving the performance of anaerobic co-digestion and the fertilizer utilization of digestate in the co-digestion systems.


Assuntos
Aloe , Esterco , Silicatos de Alumínio , Anaerobiose , Biocombustíveis , Reatores Biológicos , Metano , Fósforo
11.
Cerebrovasc Dis ; 49(1): 88-96, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31995799

RESUMO

BACKGROUND: The statistical association between a short-term rise in low-density lipoprotein cholesterol (LDL-C) levels and the short-term outcome of acute ischemic stroke remains unknown. We aimed to evaluate the association in acute ischemic stroke patients during hospitalization. METHODS: Patients with acute ischemic stroke who received statin at discharge were enrolled in this multicenter registry study. LDL-C values were measured on the first day after admission and on the day before discharge to determine the rise in LDL-C levels. Poor outcome was defined as a modified Ranking Scale score ≥2 at discharge. The National Institutes of Health Stroke Scale increase from admission to discharge by 2 points was defined as clinical deterioration. Logistic regression analyses were used to analyze the relationship between LDL-C rise during hospitalization and poor outcome at discharge. Variables that were significantly different between the LDL-C rise and LDL-C fall groups were considered in adjustment for confounding variables in model 1. Age, sex, and those variables in model 1 were considered in adjustment for confounding variables in model 2. RESULTS: Among the 676 patients, 110 (16.3%) showed a rise in LDL-C levels during hospitalization. Multivariate analyses showed that LDL-C at admission <1.6 mmol/L was significantly correlated with LDL-C rise during hospitalization (p < 0.001). There were significantly more patients with a poor outcome in the "LDL-C rise" group than in the "LDL-fall" group (p = 0.002). Multiple models consistently showed that LDL-C rise increased the risk of a poor outcome at discharge in model 1 (OR [95% CI] 1.351 [1.059-1.723], p = 0.016) and model 2 (OR [95% CI] 1.370 [1.071-1.751], p = 0.012). LDL-C rise also increased the risk of clinical deterioration, although its p value only was 0.043 in model 1 and 0.048 in model 2. CONCLUSIONS: Rise in LDL-C during hospitalization from acute ischemic stroke is an independent predictor of poor outcome at discharge. In particular, patients with lower LDL-C values at admission are a higher at risk, and LDL-C in these patients should thus be monitored while in hospital.

12.
Aging (Albany NY) ; 12(2): 1366-1376, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31967976

RESUMO

BACKGROUND: Autophagy is a self-digesting process that can satisfy the metabolic needs of cells, and is closely related to development of cancer. However, the effect of autophagy-related genes (ARGs) on the prognosis of breast cancer remains unclear. RESULTS: We first found that 27 ARGs were significantly associated with overall survival in breast cancer. The prognosis-related ARGs signature established using the Cox regression model consists of 12 ARGs that can be divided patients into high-risk and low-risk groups. The overall survival of patients with high-risk scores (HR 3.652, 2.410-5.533; P < 0.001) was shorter than patients with low-risk scores. The area under the receiver operating characteristic (ROC) curve for 1-year, 3-year, and 5-year survival rates were 0.739, 0.727, and 0.742, respectively. CONCLUSION: The12-ARGs marker can predict the prognosis of breast cancer and thus help individualized treatment of patients at different risks. METHODS: Based on the TCGA dataset, we integrated the expression profiles of ARGs in 1,039 breast cancer patients. Differentially expressed ARGs and survival-related ARGs were evaluated by computational difference algorithm and COX regression analysis. In addition, we also explored the mutations in these ARGs. A new prognostic indicator based on ARGs was developed using multivariate COX analysis.

13.
Plant Physiol Biochem ; 147: 280-288, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31891862

RESUMO

As one of the largest families of transcription factors in plants, the R2R3-MYB proteins play important roles in diverse biological processes including growth and development, primary and secondary metabolism such as flavonoid and anthocyanin biosynthesis as well as abiotic and biotic stress responses. However, functions of R2R3-MYB genes in rapeseed (Brassica napus L.) remain elusive. Here, we characterized BnaMYB111L, which is homologous to Arabidopsis MYB111 and encodes an R2R3-MYB protein in rapeseed. BnaMYB111L is responsive to abscisic acid (ABA), heat, cold, hydrogen peroxide and fungal pathogen Sclerotinia scelerotiorum treatments through quantitative RT-PCR assay. BnaMYB111L encodes a transcriptional activator and is localized exclusively to nuclei. Interestingly, overexpression of BnaMYB111L in tobacco (Nicotiana benthamiana) and rapeseed protoplasts promoted reactive oxygen species (ROS) production and hypersensitive response-like cell death, accumulation of malondialdehyde (MDA) as well as degradation of chlorophyll. Furthermore, BnaMYB111L expression evoked the alterations of transcript levels of genes encoding ROS-producing enzyme, vacuolar processing enzymes and genes implicated in defense responses. A further dual luciferase reporter assay indicated that BnaMYB111L activated the expression of RbohB, MC4 and ACRE132, which are involved in ROS generation, cell death as well as defense responses. Taken together, this study characterized the function of rapeseed MYB111L and identified its putative target genes involved in ROS production and cell death.

14.
Radiology ; 294(2): 299-307, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31769742

RESUMO

Background Use of contrast material-enhanced (CE) US Liver Imaging Reporting and Data System (LI-RADS) version 2017 has not been validated in large populations where hepatitis B virus (HBV) is endemic. Purpose To evaluate the diagnostic performance of CE US LI-RADS version 2017 in a population with a high prevalence of HBV infection. Materials and Methods In this retrospective study, liver nodules in patients with HBV who were evaluated from January 2004 to December 2016 were categorized as CE US LR-1 to LR-5 through LR-M. A subgroup of LR-M nodules was reclassified as LR-5, and additional analysis was performed. The reference standard consisted of histologic evaluation or composite imaging and clinical follow-up findings. Diagnostic performance was assessed with sensitivity, specificity, positive predictive value (PPV), and negative predictive value. Results A total of 2020 nodules in 1826 patients (median age, 54 years ± 12 [standard deviation]; 1642 men) were included. Of the 1159 LR-5 lesions, 1141 were hepatocellular carcinoma (HCC); three, intrahepatic cholangiocarcinomas; six, other malignancies; six, atypical hyperplasia; and three, benign lesions. The PPV of LR-5 for HCC was 98% (95% confidence interval [CI]: 98%, 99%). In LR-M nodules, 153 showed arterial phase hyperenhancement, early washout, and absence of punched-out appearance within 5 minutes, and 142 of 153 (93%; 95% CI: 89%, 97%) were HCC. If these nodules were reclassified as LR-5, LR-M specificity and PPV as a predictor of non-HCC malignancy increased from 88% (95% CI: 87%, 89%) and 36% (95% CI: 31%, 41%) to 96% (95% CI: 95%, 97%) and 58% (95% CI: 51%, 65%), respectively (P < .001). Despite reclassification, LR-5 specificity and PPV remained high (94% [95% CI: 92%, 96%] and 98% [95% CI: 97%, 99%], respectively). Conclusion The contrast-enhanced US Liver Imaging Reporting and Data System version 2017 category LR-5 is effectively predictive of the presence of hepatocellular carcinoma. In patients with hepatitis B virus infection, performance may be further improved by reclassification of category LR-M nodules with arterial phase hyperenhancement, early washout, and no punched-out appearance to LR-5. Published under a CC BY 4.0 license. Online supplemental material is available for this article. See also the editorial by Sidhu in this issue.

15.
Anal Chem ; 92(1): 1409-1415, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31829003

RESUMO

Interrogating metabolite crosstalk in live cells is important to understand the interplay between metabolic and signal transduction pathways but is challenging due to the lack of efficient analytical techniques. Here we report a sequentially activated probe design strategy resulting in probe HF-6 being capable of imaging the crosstalk between H2O2 and formaldehyde in live cells. Fluorescence of HF-6 can only be triggered by first H2O2 activation followed by binding with formaldehyde. Facilitated by this sequentially activated mechanism, HF-6 imaging revealed H2O2-induced upregulation of formaldehyde in live SH-SY5Y cells, while little change of intracellular H2O2 level was observed when cells were stimulated with formaldehyde for limited time. These results establish a link for the crosstalk between H2O2 and formaldehyde in redox signaling and provide a starting point to study broader metabolite interactions.

16.
J Exp Med ; 217(1)2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31699822

RESUMO

Blood-brain barrier (BBB) dysfunction has been suggested to play an important role in epilepsy. However, the mechanism mediating the transition from cerebrovascular damage to epilepsy remains unknown. Here, we report that endothelial cyclin-dependent kinase 5 (CDK5) is a central regulator of neuronal excitability. Endothelial-specific Cdk5 knockout led to spontaneous seizures in mice. Knockout mice showed increased endothelial chemokine (C-X-C motif) ligand 1 (Cxcl1) expression, decreased astrocytic glutamate reuptake through the glutamate transporter 1 (GLT1), and increased glutamate synaptic function. Ceftriaxone restored astrocytic GLT1 function and inhibited seizures in endothelial Cdk5-deficient mice, and these effects were also reversed after silencing Cxcl1 in endothelial cells and its receptor chemokine (C-X-C motif) receptor 2 (Cxcr2) in astrocytes, respectively, in the CA1 by AAV transfection. These results reveal a previously unknown link between cerebrovascular factors and epileptogenesis and provide a rationale for targeting endothelial signaling as a potential treatment for epilepsy.

17.
Sci Rep ; 9(1): 18248, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31796756

RESUMO

Vascular dementia (VaD) is a complex disorder caused by reduced blood flow in the brain. However, there is no effective pharmacological treatment option available until now. Here, we reported that low-dose levamlodipine besylate could reverse the cognitive impairment in VaD mice model of right unilateral common carotid arteries occlusion (rUCCAO). Oral administration of levamlodipine besylate (0.1 mg/kg) could reduce the latency to find the hidden platform in the MWM test as compared to the vehicle group. Furthermore, vehicle-treated mice revealed reduced phospho-CaMKII (Thr286) levels in the hippocampus, which can be partially restored by levamlodipine besylate (0.1 mg/kg and 0.5 mg/kg) treatment. No significant outcome on microglia and astrocytes were observed following levamlodipine besylate treatment. This data reveal novel findings of the therapeutic potential of low-dose levamlodipine besylate that could considerably enhance the cognitive function in VaD mice.

18.
Cell Death Differ ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31819157

RESUMO

Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays a critical role in many fundamental cellular processes. We recently reported that PP6 is essential for female fertility. Here, we report that PP6 is involved in meiotic recombination and that germ cell-specific deletion of PP6 by Stra8-Cre causes defective spermatogenesis. The PP6-deficient spermatocytes were arrested at the pachytene stage and defects in DSB repair and crossover formation were observed, indicating that PP6 facilitated meiotic double-stranded breaks (DSB) repair. Further investigations revealed that depletion of PP6 in the germ cells affected chromatin relaxation, which was dependent on MAPK pathway activity, consequently preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. Taken together, our results demonstrate that PP6 has an important role in meiotic recombination and male fertility.

19.
Chin Med J (Engl) ; 132(24): 2899-2904, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31855969

RESUMO

BACKGROUND: Clinical outcomes of undifferentiated arthritis (UA) are diverse, and only 40% of patients with UA develop rheumatoid arthritis (RA) after 3 years. Discovering predictive markers at disease onset for further intervention is critical. Therefore, our objective was to analyze the clinical outcomes of UA and ascertain the predictors for RA development. METHODS: We performed a prospective, multi-center study from January 2013 to October 2016 among Chinese patients diagnosed with UA in 22 tertiary-care hospitals. Clinical and serological parameters were obtained at recruitment. Follow-up was undertaken in all patients every 12 weeks for 2 years. Predictive factors of disease progression were identified using multivariate Cox proportional hazards regression. RESULTS: A total of 234 patients were recruited in this study, and 17 (7.3%) patients failed to follow up during the study. Among the 217 patients who completed the study, 83 (38.2%) patients went into remission. UA patients who developed RA had a higher rheumatoid factor (RF)-positivity (42.9% vs. 16.8%, χ = 8.228, P = 0.008), anti-cyclic citrullinated peptide (CCP) antibody-positivity (66.7% vs. 10.7%, χ = 43.897, P < 0.001), and double-positivity rate of RF and anti-CCP antibody (38.1% vs. 4.1%, χ = 32.131, P < 0.001) than those who did not. Anti-CCP antibody but not RF was an independent predictor for RA development (hazard ratio 18.017, 95% confidence interval: 5.803-55.938; P < 0.001). CONCLUSION: As an independent predictor of RA, anti-CCP antibody should be tested at disease onset in all patients with UA.

20.
J Mater Chem B ; 7(47): 7558-7565, 2019 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-31724692

RESUMO

Actuating materials can convert external stimuli (humidity, light, electricity, etc.) into mechanical energy and realize multiple forms of movements. However, a majority of current actuating materials are driven by a single stimulus with a small degree of actuation and rough control which is unfavorable for practical applications. Here, a new type of bidirectional actuating material based on carbon nanotube/PDMS composites and chitosan films is proposed. Thanks to the robust mechanical support by PDMS, due to the ultra-large water capacity in between chitosan chains and strong near-infrared light absorption by carbon nanotube layers, the actuator can be driven by humidity and light for an ultra-large actuation curvature (3.91 cm-1 in humidity actuation, 3.84 cm-1 in light actuation). The well-established light power-curvature, relative humidity-curvature profiles and a fine mechanic modelling of the actuator show the possibility of controlling the actuator's bending. A lab application as a cargo-moving device preliminarily demonstrates a robust mechanical functionality of this actuator with a low body weight.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA