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1.
Curr Pain Headache Rep ; 25(6): 37, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33821366

RESUMO

Trigeminal neuralgia (TN) is a disease of unclear pathogenesis. It has a low incidence and is not fatal, but it can cause afflicted patients' depression or suicide. In the past, neurovascular compression was considered to be the main cause of TN, but recent studies have found that neurovascular contact is also common in asymptomatic patients and the asymptomatic side in symptomatic patients. This indicates that the neurovascular contact is not, or is only to a lesser extent, a factor in the development of TN. Thus, the study of the peripheral branches of the trigeminal nerve is necessary to understand the etiology of TN. With the development of imaging technology and the emergence of various imaging modalities, it is possible to study the etiology of TN and the pathological changes of related structures by magnetic resonance neuroimaging. This article reviews the recent advances in magnetic resonance neuroimaging of the trigeminal nerve.

2.
Cereb Cortex ; 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33822908

RESUMO

The brain exhibits highly organized patterns of spontaneous activity as measured by resting-state functional magnetic resonance imaging (fMRI) fluctuations that are being widely used to assess the brain's functional connectivity. Some evidence suggests that spatiotemporally coherent waves are a core feature of spontaneous activity that shapes functional connectivity, although this has been difficult to establish using fMRI given the temporal constraints of the hemodynamic signal. Here, we investigated the structure of spontaneous waves in human fMRI and monkey electrocorticography. In both species, we found clear, repeatable, and directionally constrained activity waves coursed along a spatial axis approximately representing cortical hierarchical organization. These cortical propagations were closely associated with activity changes in distinct subcortical structures, particularly those related to arousal regulation, and modulated across different states of vigilance. The findings demonstrate a neural origin of spatiotemporal fMRI wave propagation at rest and link it to the principal gradient of resting-state fMRI connectivity.

3.
Arch Microbiol ; 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33797591

RESUMO

A bacterial strain ODT-83 is isolated from oysters, which is capable of adsorbing norovirus (NoV) via histo-blood group antigen-like (HBGA-like) substances. To better understand its genetic background associated with the production of HBGA-like substances, the genome of the ODT-83 was completely sequenced and analyzed. The ODT-83 only contains one circular chromosome, with a length of 5,384,159 bp. Both the 16S rRNA gene phylogeny and the average nucleotide identity (ANI) analyses confirm that the ODT-83 is a new Pseudomonas oleovorans strain. The whole genome encodes a total of 5037 predicted open reading frames (ORFs), 66 tRNA genes and 12 rRNA genes. Two gene clusters are detected on the genome, which are involved in the synthesis of polysaccharides of alginate and Pel, respectively. These results lay the foundation for further research on the interaction between the P. oleovorans strain ODT-83 and NoV.

4.
Pharmacol Res ; : 105601, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33838294

RESUMO

Sucrose preference test (SPT) is a most frequently applied method for measuring anhedonia, a core symptom of depression, in rodents. However, the method of SPT still remains problematic mainly due to the primitive, irregular, and inaccurate various types of home-made equipment in laboratories, causing imprecise, inconsistent, and variable results. To overcome this issue, we devised a novel method for automatic detection of anhedonia in mice using an electronic apparatus with its program for automated detecting the behavior of drinking of mice instead of manual weighing the water bottles. In this system, the liquid surface of the bottles was monitored electronically by infrared monitoring elements which were assembled beside the plane of the water surface and the information of times and duration of each drinking was collected to the principal machine. A corresponding computer program was written and installed in a computer connected to the principal machine for outputting and analyzing the data. This new method, based on the automated system, was sensitive, reliable, and adaptable for evaluation of stress- or drug-induced anhedonia, as well as taste preference and effects of addictive drugs. Extensive application of this automated apparatus for SPT would greatly improve and standardize the behavioral assessment method of anhedonia, being instrumental in novel antidepressant screening and depression researching.

5.
Medicine (Baltimore) ; 100(11): e24315, 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33725930

RESUMO

ABSTRACT: Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, considerable attention has been paid on its epidemiology and clinical characteristics in children patients. However, it is also crucial for clinicians to summarize and investigate the co-infection of SARS-CoV-2 in children.We retrospectively reviewed the clinical manifestations, laboratory findings, and imaging characteristics of COVID-19 patients in co-infection group (CI, n = 27) and single infection group (SI, n = 54). Samples were tested for multiple pathogens.A high incidence (27/81, 33%) of co-infection in children with COVID-19 was revealed. The most frequent co-infected pathogen was mycoplasma pneumoniae (MP, 20/81, 25%), followed by virus (6/81, 7%), and bacteria (4/81, 5%). No significant difference in clinical characteristics, laboratory examinations, or hospital stay was observed between the patients with co-infections and those with monomicrobial, only lower in white blood cell counts (CI: 5.54 ±â€Š0.36 vs SI: 7.38 ±â€Š0.37, P = .002), neutrophil counts (CI: 2.20 ±â€Š0.20 vs SI: 2.92 ±â€Š0.23, P = .024) and lymphocyte counts (CI: 2.72 ±â€Š0.024 vs SI: 3.87 ±â€Š0.28, P = .006). Compared with the patients with monomicrobial, chest imaging of those with co-infections showed consolidation in more cases (CI: 29.6% vs SI: 11.1%, P = .038) and duration of positive in nucleic acid was shorter (CI: 6.69 ±â€Š0.82 vs SI: 9.69 ±â€Š0.74, P = .015).Co-infection was relatively common in children with COVID-19, almost 1/3 had co-infection, most commonly caused by MP. Co-infection did not cause a significant exacerbation in clinical manifestations.


Assuntos
/epidemiologia , Coinfecção/epidemiologia , Infecções por Adenoviridae/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Coinfecção/microbiologia , Coinfecção/virologia , Feminino , Humanos , Influenza Humana/epidemiologia , Contagem de Leucócitos , Contagem de Linfócitos , Masculino , Infecções por Moraxellaceae/epidemiologia , Mycoplasma pneumoniae , Infecções Pneumocócicas/epidemiologia , Pneumonia por Mycoplasma/epidemiologia , Infecções por Vírus Respiratório Sincicial/epidemiologia , Estudos Retrospectivos
6.
Environ Pollut ; 279: 116885, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33744634

RESUMO

It is still a great challenge to address nutrient pollution issues caused by various point sources and non-point sources on the watershed scale. Source contribution analysis based on watershed modeling can help watershed managers identify major pollution sources, propose effective management plans and make smart decisions. This study demonstrated a technical procedure for addressing watershed-scale water pollution problems in an agriculture-dominated watershed, using the Dengsha River Watershed (DRW) in Dalian, China as an example. The SWAT model was improved by considering the constraints of soil nutrient concentration, i.e., nitrogen (N) and phosphorus (P), when modeling the nutrient uptake by a typical crop, corn. Then the modified SWAT model was used to quantify the contributions of all known pollution sources to the N and P pollution in the DRW. The results showed that crop production and trans-administrative wastewater discharge were the two dominant sources of nutrient pollution. This study further examined the responses of nutrient loss and crop yield to different fertilizer application schemes. The results showed that N fertilizer was the limiting factor for crop yield and that excessive levels of P were stored in the agricultural soils of the DRW. An N fertilizer application rate of approximately 40% of the current rate was suggested to balance water quality and environmental protection with crop production. The long-term impact of legacy P was investigated with a 100-year future simulation that showed the crop growth could maintain for 12 years even after P fertilization ceased. Our study highlights the need to consider source attribution, fertilizer application and legacy P impacts in agriculture-dominated watersheds. The analysis framework used in this study can provide a scientifically sound procedure for formulating adaptive and sustainable nutrient management strategies in other study areas.

7.
Biomed Pharmacother ; 137: 111322, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33761592

RESUMO

Autism spectrum disorder (ASD) is a sort of mental disorder marked by deficits in cognitive and communication abilities. To date no effective cure for this pernicious disease has been available. Valproic acid (VPA) is a broad-spectrum, antiepileptic drug, and it is also a potent teratogen. Epidemiological studies have shown that children exposed to VPA are at higher risk for ASD during the first trimester of their gestational development. Several animal and human studies have demonstrated important behavioral impairments and morphological changes in the brain following VPA treatment. However, the mechanism of VPA exposure-induced ASD remains unclear. Several factors are involved in the pathological phase of ASD, including aberrant excitation/inhibition of synaptic transmission, neuroinflammation, diminished neurogenesis, oxidative stress, etc. In this review, we aim to outline the current knowledge of the critical pathophysiological mechanisms underlying VPA exposure-induced ASD. This review will give insight toward understanding the complex nature of VPA-induced neuronal toxicity and exploring a new path toward the development of novel pharmacological treatment against ASD.

8.
Opt Lett ; 46(6): 1389-1392, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33720194

RESUMO

Manipulation of spoof surface plasmons (SSPs) has recently intrigued enormous interest due to the capability of guiding waves with subwavelength footsteps. However, most of the previous studies, manifested for a single functionality, are not suitable for multifunctional integrated devices. Herein, a bifunctional Luneburg-fish-eye lens is proposed based on a 2D metal pillar array. First, by tuning the dimension of the metal pillars in the array, its ability to precisely manipulate the SSPs along one direction is confirmed, achieving subwavelength focusing and imaging with a resolution up to 0.14λ. Then, separately controlling the propagation of the SSPs along the orthotropic directions is further implemented, and the bifunctional Luneburg-fish-eye lens is realized. It is experimentally characterized as a Luneburg lens along the x axis, whereas in the y axis, it presents the properties of a Maxwell fish-eye lens. This bifunctional lens can reduce the system complexity and exert flexibility in multifunctional applications, while the proposed metal pillar-based design method broadens the application range of the gradient refractive-index lens in microwave, terahertz, and even optical ranges.

9.
ACS Appl Mater Interfaces ; 13(10): 11657-11671, 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33684289

RESUMO

Glioblastoma is the most destructive type of brain cancer. The blood-brain barrier (BBB) is a tremendous obstacle that hinders therapeutic agents, such as chemical drugs and antibodies, from reaching glioblastoma tissues. Meanwhile, the abnormal microenvironment of glioblastoma extremely restricts the expected therapeutic effects of accumulated drugs. Therefore, in the present study, BBB-regulating nanovesicles (BRN) are developed to achieve targeted and controlled BBB regulation, carrying adenosine 2A receptor (A2AR) agonists and perfluorocarbon (PF). The red-blood-cell membrane (RBCM) is included on the outside to avoid the premature release of therapeutic agents. In the presence of ultrasonication (US), A2AR agonists are released and induce effects on both F-actin and tight junctions of endothelial cells. Subsequently, BBB permeability is temporarily increased and enables small molecules and nanoparticles to enter brain parenchymal tissues. The high affinity between manganese dioxide and temozolomide (TMZ) is utilized to form multifunctional nanoparticles to ameliorate the hypoxic microenvironment, which yields improved glioblastoma inhibition combined with radiotherapy. Moreover, with the aid of targeted BBB regulation, programmed death ligand-1 (PD-L1) antibody induces a tumor-specific immune response. Taken together, the findings suggest that synergistic combination may have the potential in amplifying the therapeutic efficacies of clinical drugs and immune checkpoint blockade antibodies to overcome the therapeutic resistance of glioblastoma.

10.
Biol Trace Elem Res ; 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33738684

RESUMO

It is rapidly increasing to have selenium (Se) supplementation for urban elderly population in China since they are facing a widespread deficiency daily Se intake. However, until now, there is no low-cost, non-invasive, rapid, and reliable method to monitor the health improvement or risk for elderly Se-supplemented population in China. The present cross-sectional study (229 participants with older than 55 years old) performed in Beijing, China, revealed that the Se concentrations of non-supplementer users (n = 27) were 55 ± 23 µg/L in urine, 139.9 ± 102.3 µg/L in serum, and 487.6 ± 158.7 µg/kg in hair. But a significant increase on hair Se concentrations (615.4 ± 238.8 µg/kg) was observed for Se supplementer users (n = 202) (p < 0.05); there were no significant statistical differences in serum and urine between the Se-supplemented (n = 202) and Se non-supplemented groups (n = 27). This indicated the hair Se levels could be a more sensitive biomarker for Se-supplemented elderly population. Participants who consumed Se supplements for 7-12 months had the highest Se status based on hair and serum Se concentrations (p < 0.05). The present study also revealed that most elderly adults in Beijing just need to supplement 50 µg Se per day to achieve Se plateau status. Furthermore, hair Se levels were positively related with triglycerides/TG levels (p < 0.05) but not body mass index/BMI, total cholesterol/TC, and low-density lipoprotein cholesterol/LDL, implicating Se supplementation for Se sufficiency baseline in elderly population in Beijing likely posed health risk, especially on TG because of excessive Se oxidation stress. An ongoing monitoring of Se status via hair is still warranted to prevent future Se deficiency or excess in China.

11.
J Cell Physiol ; 236(7): 5352-5361, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33586215

RESUMO

In vitro culture of follicles is a promising technology to generate large quantities of mature oocytes and it could offer a novel option of assisted reproductive technologies. Here we described a 2-dimensional follicular serum-free culture system with 3-dimensional effect that can make secondary follicles develop into antral follicles (78.52%), generating developmentally mature oocytes in vitro (66.45%). The oocytes in this serum-free system completed the first meiosis; spindle assembly and chromosome congression in most oocytes matured from follicular culture were normal. However, these oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca2+ oscillations was also lower in response to parthenogenetic activation, after which a 2-cell embryonic developmental block occurred. Oocytes matured from follicular culture displayed increased abnormal mitochondrial distribution and increased reactive oxygen species levels when compared to in vivo matured oocytes. These data are important for understanding the reasons for reduced developmental potential of oocytes matured from follicular culture, and for further improving the cultivation system.

12.
Int J Mol Med ; 47(4): 1, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33576457

RESUMO

As previously reported, long intergenic non­protein­coding RNA 1006 (LINC01006) plays crucial roles in prostate, pancreatic and gastric cancers. However, whether it plays important roles in cervical cancer remains unclear. The present study thus aimed to determine the precise role of LINC01006 in cervical cancer and elucidate its regulatory mechanisms. The expression of LINC01006 in cervical cancer was examined by reverse transcription­quantitative polymerase chain reaction. Cell proliferation assay, flow cytometric analysis, Transwell migration and invasion assays, and tumor xenograft model experiments were performed to elucidate the roles of LINC01006 in cervical cancer. Bioinformatics analysis, luciferase reporter assay, RNA immunoprecipitation and rescue experiments were performed for mechanistic analyses. The expression of LINC01006 was found to be upregulated in cervical cancer and to be associated with a poor prognosis. The absence of LINC01006 inhibited the proliferation, migration and invasion of cervical cancer cells, whereas it promoted cell apoptosis in vitro. The downregulation of LINC01006 impeded tumor growth in vivo. LINC01006 was verified as an endogenous 'sponge' that competed for microRNA­28­5p (miR­28­5p), which resulted in the upregulation of the miR­28­5p target P21­activated kinase 2 (PAK2). Rescue experiments revealed that the suppression of miR­28­5p expression or the overexpression of PAK2 abrogated the effects of LINC01006 downregulation on malignant cellular functions in cervical cancer. On the whole, the present study demonstrates that LINC01006 exhibits tumor­promoting functions in cervical cancer via the regulation of the miR­28­5p/PAK2 axis. These findings may provide the basis for the identification of LINC01006­targeted clinical therapy.

13.
Protein Expr Purif ; 182: 105840, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33561520

RESUMO

Hyaluronate lyases have received extensive attention due to their applications in medical science, drug and biochemical engineering. However, few thermotolerant and pH-stable hyaluronate lyases have been found. In this study, hyaluronate lyase TcHly8B from Thermasporomyces composti DSM22891 was expressed in Escherichia coli BL21(DE3), purified, and characterized. Phylogenetic analysis revealed that TcHly8B belonged to a new subfamily in PL8. The molecular mass of recombinant TcHly8B determined by SDS-PAGE was approximately 86 kDa. The optimal temperature of TcHly8B was 70 °C, which was higher than that of previously reported hyaluronate lyases. TcHly8B was very stable at temperatures from 0 to 60 °C. The optimal pH of TcHly8B was 6.6. It could retain more than 80% of its original enzyme activity after incubation for 12 h in the pH range of 3.0-10.6. TcHly8B degraded hyaluronic acid into unsaturated disaccharides as the end products. The amino acid sequence and structure analysis of TcHly8B demonstrated that the amino acid composition and salt bridges might contribute to the thermostability of TcHly8B. Overall, this study provides an excellent example for the discovery of thermotolerant hyaluronate lyases and can be applied to the industrialized production and basic research of hyaluronate oligosaccharides.

14.
Wei Sheng Yan Jiu ; 50(1): 100-103, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33517969

RESUMO

OBJECTIVE: To observe the effect of selenomethionine(SeMet)on the selenoproteins expression in hepatocyte L02 and the synergistic effect of serine. METHODS: The L02 cells were cultured and divided into SeMet group and Serine+SeMet group. SeMet dose was set as 0. 001, 0. 01, 0. 1, 1 and 10 µmol/L. Serine and SeMet were mixed according to 2∶1 molar ratio(Serine∶SeMet=2∶1). The L02 cells were cultured for 48 h after SeMet and Serine added. Finally, the cell culture supernatants and homogenates were collected for the selenoprotein P(SEPP)and glutathione peroxidase 1(GPx1)concentrations detection by a double-antibody sandwich enzyme-linked immuno-sorbent assay(ELISA). The expression of SEPP and GPx1 in cell homogenates was detected by western blot(WB). RESULTS: ELISA and WB results showed that GPx1 and SEPP expressions were dose dependent in a low SeMet concentration range, reached their inflection points when SeMet concentration was 1 µmol/L and 0. 1 µmol/L respectively, and began to decrease when SeMet concentration was further increased to 10 µmol/L. WB result showed that serine had a synergistic effect on GPx1 and SEPP expressions as SeMet concentration was 0. 001 µmol/L to 10 µmol/L. CONCLUSION: There was an inflection point in selenium protein synthesis when SeMet acted on normal hepatocyte(L02), the specific mechanism needs to be further explored. Secondly, serine had a synergistic effect on GPx1 and SEPP expressions of SeMet in hepatocyte(L02).


Assuntos
Selênio , Selenometionina , Glutationa Peroxidase/genética , Hepatócitos , Selenoproteínas/genética , Serina
15.
Nanoscale ; 13(7): 4249-4261, 2021 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-33595022

RESUMO

Conventional prostate cancer treatment strategies, including chemotherapy and radiotherapy, cannot effectively eradicate prostate cancer, especially castration resistance prostate cancer. Herein, we developed a novel nanotherapy platform that consists of synergic photothermal and photodynamic therapy via the unique properties of photothermal conversion by gold nanorods and free radicals generation by encapsulated initiators (AIPH). Mesoporous silica was employed as a coating material, and the bombesin peptide was conjugated onto the mesoporous silica coating layer as the targeting moiety to prostate cancer via its overexpressed gastrin-releasing peptide receptors. An in vitro study with the castration resistance prostate cancer cell exhibited a significant photothermal therapeutic effect as well as enhanced thermodynamic therapy via generating free radicals. P-p38 and p-JNK proteins, as key proteins involved in the cells' stress responses, were found to be upregulated by the synergetic treatment. The in vivo study demonstrated that a significant eradication of prostate tumour could be achieved by the nanoparticle therapeutic platform with a good biocompatibility profile. This work pioneers a novel approach for high-efficient castration resistance prostate cancer treatment by combining photothermal, thermodynamic, and site-specific drug delivery directed by an integrated nanoparticle system.

16.
Curr Med Imaging ; 2021 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-33632107

RESUMO

INTRODUCTION: Although by Computed Tomography (CT) is the most convenient technology for the diagnosis of gastric lipoma, it also has a high rate of missed diagnosis of gastric lipoma. OBJECTIVE: To analyze the causes of missed diagnosis of gastric lipomas by CT. METHOD: We retrospectively studied the CT images and CT diagnosis reports of 25 cases of gastric lipoma confirmed by surgery or clinical follow-up at the Affiliated Hospital of North Sichuan Medical College and Nanchong Central Hospital from 2016 to 2020, and analyzed the causes of missed diagnosis of gastric lipomas. RESULTS: Among the 25 cases of gastric lipomas included in this study, 17 cases (68.0%) were correctly diagnosed by CT and 8 cases (32.0%) were missed, but there was no case of misdiagnosis. 18 cases (72.0%) of gastric lipomas were located in the gastric antrum, 2 cases (8.0%) at the junction of the gastric body and antrum, 5 cases (20.0%) at the fundus of the stomach, 23 cases (92.0%) under the gastric mucosa, and 2 cases (8.0 %) under the gastric serous membrane. All gastric lipoma cases were manifested as round or oval-shaped low-density shadows with clear boundaries on CT. 22 cases (88.0%) showed homogeneous low-density shadows while 3 cases (12.0%) mainly showed low-density shadows containing medium-density strips. There was no obvious enhancement in the contrast enhanced CT scan. The gastric lipoma cases missed by CT were all located under the gastric mucosa of the gastric antrum. When reading the CT images on the default upper abdominal window width and window level, all the missed lesions were similar to the gas image. And the straight meridian of three lesions was less than 2 cm. CONCLUSION: Fat density shadow in gastric antrum area mistaken for gastrointestinal gas. Improper CT image window width and window levels, and small gastric lipoma volume, along with insufficient knowledge of gastric lipomas imaging by the clinician might be the main causes of missed diagnosis of gastric lipomas by CT. Familiarity with the CT manifestations of gastric lipomas and rich clinical experience can improve the rate of correct diagnosis of gastric lipomas by CT.

17.
J Clin Invest ; 131(4)2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33586674

RESUMO

Abnormal angiogenesis and regression of the diseased retinal vasculature are key processes associated with ischemic retinopathies, but the underlying mechanisms that regulate vascular remodeling remain poorly understood. Here, we confirmed the specific expression of semaphorin 3G (Sema3G) in retinal endothelial cells (ECs), which was required for vascular remodeling and the amelioration of ischemic retinopathy. We found that Sema3G was elevated in the vitreous fluid of patients with proliferative diabetic retinopathy (PDR) and in the neovascularization regression phase of oxygen-induced retinopathy (OIR). Endothelial-specific Sema3G knockout mice exhibited decreased vessel density and excessive matrix deposition in the retinal vasculature. Moreover, loss of Sema3G aggravated pathological angiogenesis in mice with OIR. Mechanistically, we demonstrated that HIF-2α directly regulated Sema3G transcription in ECs under hypoxia. Sema3G coordinated the functional interaction between ß-catenin and VE-cadherin by increasing ß-catenin stability in the endothelium through the neuropilin-2 (Nrp2)/PlexinD1 receptor. Furthermore, Sema3G supplementation enhanced healthy vascular network formation and promoted diseased vasculature regression during blood vessel remodeling. Overall, we deciphered the endothelium-derived Sema3G-dependent events involved in modulating physiological vascular remodeling and regression of pathological blood vessels for reparative vascular regeneration. Our findings shed light on the protective effect of Sema3G in ischemic retinopathies.

18.
Transpl Immunol ; 65: 101371, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33545333

RESUMO

INTRODUCTION: Acute renal rejection usually fails to be diagnosed before the increase in the serum creatinine levels, and the resultant damage to the renal tissues occur in varying degrees. We hypothesized that the combined detection of human leucocyte antigen-G (HLA-G) 14-bp insertion/deletion genotypes and kidney injury molecule-1 (KIM-1) and osteopontin (OPN) levels in serum might facilitate the prediction of acute renal allograft rejections in kidney transplant recipients. METHODS: HLA-G 14-bp insertion/deletion genotypes and the serum KIM-1 and OPN levels of 77 kidney transplant recipients were determined and compared before operation and on days 1, 4, and 7 after the operation (32 in acute rejection [AR] group and 45 in stable allograft function [STA] group). These 3 indicators were combined to establish a model for the early prediction of AR. RESULTS: The KIM-1 levels in the serum of patients were significantly higher in the AR group than in the STA group. The area under the receiver operator characteristics (ROC) curve (AUC) of KIM-1 for the prediction of rejection was maximized on the1st day after operation, with a sensitivity of 84.4% and a specificity of 86.7%. The OPN levels in the serum of patients were significantly higher in the AR group than in the STA group only before operation and on the 7th day after operation. The AUC of OPN for the prediction of rejection was maximized on 7th day after operation, with a sensitivity of 68.8% and a specificity of 88.9%. The HLA-G + 14-bp allele frequency was also significantly higher in the AR group than in the STA group. The results of these three indicators were converted into a qualitative method. If any two of the three indicators show as positive, it was diagnosed as acute rejection, and it has the highest ability to predict acute rejection with a sensitivity and specificity of 84.38% and 91.11%, respectively. CONCLUSIONS: The HLA-G 14-bp insertion/deletion genotype and KIM-1 and OPN levels in the patients' serum were significantly different between the AR and STA groups. The power of predicting acute renal allograft rejection could be improved by combined these three biomarkers.

19.
Anal Biochem ; : 114140, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33610535

RESUMO

A facile, sensitive and unmodified Hg2+ homogeneous electrochemical sensor based on bisferrocene signal markers and catalytic hairpin self-assembly (CHA) was built on a gold disk electrode. Three hairpin probes were designed, in which thiol was labeled at both ends of the hairpin probe 1(HP1), while bisferrocene, a redox signal marker, was labeled at both ends of the hairpin probe 2(HP2) and hairpin probe 3(HP3). Due to the Hg2+ mediated thymine-Hg (II)-thymine (T-Hg2+-T) structure, when Hg2+ is introduced, the T-Hg2+-T that occurred between the probe DNA and helper DNA could open the hairpin structure of probe DNA and form a rigid DNA triangles structure by CHA. Simultaneously, four bisferrocene signal markers also reached the surface of the electrode and built potential-assisted Au-S self-assembly to achieve signal amplification. Under the optimized condition, the sensor can achieve good electrochemical response Hg2+detection, and the detection limit is as low as 0.6 pM. furthermore, this sensor has high selectivity for Hg2+ detection.

20.
Ann Hum Biol ; : 1-8, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33461338

RESUMO

BACKGROUND: The sequence polymorphisms of mitochondrial DNA (mtDNA) are valuable in forensic medicine and anthropological genetics. AIM: We analysed the sequences of the mtDNA control region in 207 unrelated Tibetan individuals from the Naqu region, Tibet Autonomous Region in the People's Republic of China, and investigated the population structure of the region by population comparison with other groups. SUBJECTS AND METHODS: Genomic DNA was extracted and hypervariable regions (HVS-I and HVS-II) were amplified and sequenced. Subsequently, sequences were aligned and compared with the revised Cambridge sequence. Moreover, population comparison was performed between the Naqu Tibetan group and the other groups. CONCLUSION: Our study provided available data for exploring the mtDNA haplotype of the Tibetan population in the Naqu region, and population comparisons found that the Naqu Tibetan population has its own unique structure.

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