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1.
Cell Rep ; 38(2): 110205, 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-34982968

RESUMO

Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase in genetic diversity over time after symptom onset in individuals. Nonsynonymous mutations are overrepresented in the pool of iSNVs but underrepresented at the single-nucleotide polymorphism (SNP) level, suggesting a two-step fitness selection process: a large number of nonsynonymous substitutions are generated in the host (positive selection), and these substitutions tend to be unfixed as SNPs in the population (negative selection). Dynamic iSNV changes in subpopulations with different gender, age, illness severity, and viral shedding time displayed a varied fitness selection process among populations. Our study highlights that iSNVs provide a mutational pool shaping the rapid global evolution of the virus.

2.
J Colloid Interface Sci ; 607(Pt 2): 1239-1252, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34583031

RESUMO

Tissue adhesives have received much attention for their effectiveness in sealing wounds or incisions in clinical surgery, especially in minimally invasive surgery. To meet the safe and smart wound management requirements, ideal tissue adhesives are expected to have high biocompatibility, and be able to accelerate wound closing and healing, and monitor wound healing process. However, few adhesives fit all of the above descriptions. It has been demonstrated that inorganic nanoparticles can directly glue biological tissue based on nano-bridging effect. In this study, self-luminescence porous silicon (LPSi) particles were prepared with degradable and biocompatible properties. In addition, the self-luminescence property of LPSi particles was discovered by In Vivo Imaging System (IVIS) for the first time, which can avoid the limitations of photoluminescence imaging. Due to the oxidation and degradation reaction, LPSi particles not only can be degraded completely in several days, but also showed satisfactory biocompatibility. And their degradation product could promote tube formation of HUVECs. Moreover, owing to the high specific surface area and the outer oxide layer of LPSi particles, LPSi tissue adhesive exhibited strong adhesive strength to pig livers. Furthermore, this adhesive closed wound rapidly, promoted angiogenesis and epidermal regeneration, and facilitated wound healing in a mouse skin incision model. Importantly, the wound healing ratio can be monitored by measuring the self-luminescence intensity of LPSi particles in the wound site. This study reveals that LPSi particles could be employed as a safe and smart wound management tissue adhesive for wound closure, as well as accelerating and monitoring wound healing.


Assuntos
Adesivos Teciduais , Animais , Luminescência , Camundongos , Porosidade , Silício , Suínos , Cicatrização
3.
IEEE Trans Pattern Anal Mach Intell ; 44(1): 129-142, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32750798

RESUMO

This paper addresses the problem of photometric stereo, in both calibrated and uncalibrated scenarios, for non-Lambertian surfaces based on deep learning. We first introduce a fully convolutional deep network for calibrated photometric stereo, which we call PS-FCN. Unlike traditional approaches that adopt simplified reflectance models to make the problem tractable, our method directly learns the mapping from reflectance observations to surface normal, and is able to handle surfaces with general and unknown isotropic reflectance. At test time, PS-FCN takes an arbitrary number of images and their associated light directions as input and predicts a surface normal map of the scene in a fast feed-forward pass. To deal with the uncalibrated scenario where light directions are unknown, we introduce a new convolutional network, named LCNet, to estimate light directions from input images. The estimated light directions and the input images are then fed to PS-FCN to determine the surface normals. Our method does not require a pre-defined set of light directions and can handle multiple images in an order-agnostic manner. Thorough evaluation of our approach on both synthetic and real datasets shows that it outperforms state-of-the-art methods in both calibrated and uncalibrated scenarios.

4.
Am J Transl Res ; 13(10): 11513-11521, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34786077

RESUMO

Deep learning (DL)-based convolutional neural networks facilitate more accurate detection and rapid analysis of MLS. Our objective was to assess the feasibility of applying a DL-based convolutional neural network to non-contrast computed tomography (CT) for automated 2D/3D brain midline shift measurement and outcome prediction after spontaneous intracerebral haemorrhage. In this retrospective study, 140 consecutive patients were referred for CT assessment of sICH from January 2014 to April 2019. The level of consciousness of patients was evaluated using the Glasgow Coma Scale (GCS) score, and the Glasgow Outcome Scale (GOS) score was calculated to classify the outcome. The distance of midline shift (MLS-D) and volume of midline shift (MLS-V) were automatically measured via DL methods. Patients were divided into three groups based on GCS scores: mild degree (GCS score: 13-15), moderate degree (GCS score: 9-12), and severe degree (GCS score: 3-8). Spearman's correlation analysis revealed statistically significant (P<0.01) positive correlation between GCS and MLS-D (r=0.709) and MLS-V (r=0.754). The AUC of MLS-V was slightly larger than that of MLS-D (0.831 vs 0.799, P=0.318) in the midline shifting group. The AUC of MLS-V was significantly larger than that of MLS-D (0.854 vs 0.736, P=0.03) in patients with severe degree GCS scores. The DL-based measurements of both MLS-D and MLS-V enable the assessment of consciousness and the prediction of the outcome of sICH. Compared to MLS-D, MLS-V measurement can better indicate mass effect and predict outcomes, particularly in severe cases.

5.
Opt Express ; 29(23): 38728-38736, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34808919

RESUMO

We described a wavelength locked and spectral narrowed high-power diode laser with a Faraday anomalous dispersion optical filter (FADOF). By an external cavity with a 85Rb FADOF, the central wavelength of the diode laser was precisely locked to the Rb resonance D2 line. The bandwidth was narrowed from the free-running 4 nm to 0.002 nm (1.2 GHz, FWHM). At 4.9 A maximal driven current, the laser produced a continuous wave (CW) output of 18 W with an external cavity efficiency of 80%, either the current or the temperature had no impact on the central wavelength of the diode laser. The Rb cell works well without any damage under a long-time running. This ultra-stable and extreme-narrowed high power diode laser would find many applications in alkali lasers pumping, metastable rare gas laser pumping, spin-exchange optical pumping, and quantum optics.

6.
Front Oncol ; 11: 752158, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34745982

RESUMO

Background: Brain invasion in meningioma has independent associations with increased risks of tumor progression, lesion recurrence, and poor prognosis. Therefore, this study aimed to construct a model for predicting brain invasion in WHO grade II meningioma by using preoperative MRI. Methods: One hundred seventy-three patients with brain invasion and 111 patients without brain invasion were included. Three mainstream features, namely, traditional semantic features and radiomics features from tumor and tumor-to-brain interface regions, were acquired. Predictive models correspondingly constructed on each feature set or joint feature set were constructed. Results: Traditional semantic findings, e.g., peritumoral edema and other four features, had comparable performance in predicting brain invasion with each radiomics feature set. By taking advantage of semantic features and radiomics features from tumoral and tumor-to-brain interface regions, an integrated nomogram that quantifies the risk factor of each selected feature was constructed and had the best performance in predicting brain invasion (area under the curve values were 0.905 in the training set and 0.895 in the test set). Conclusions: This study provided a clinically available and promising approach to predict brain invasion in WHO grade II meningiomas by using preoperative MRI.

7.
Ann Nucl Med ; 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34757570

RESUMO

OBJECTIVE: Data involved the association between myocardial ischaemia and the outcome for unrevascularized coronary chronic total occlusion (CTO) patients were limited. The purpose of this study was to evaluate the predictive value of ischaemia detected by single photon emission computed tomography (SPECT) myocardial perfusion imaging (MPI) for the adverse events in unrevascularized CTO patients. We further explored whether ischaemia generated from CTO vessel can independently predict the outcome. METHODS: Patients with at least one unrevascularized CTO on coronary angiography were enrolled in this study. Exercise stress/rest SPECT MPI was performed in all patients. All patients were then followed by telephone interview and reviewing of medical records. RESULTS: Patients with ischaemia experienced significantly higher rate of adverse events than non-ischaemia patients (40.7% vs 7.1%, P = 0.002). Ischaemia demonstrated on MPI [odds ratio (OR) = 7.656; 95% confidence interval (CI) 1.598-36.677; P = 0.011] was an independent predictor for adverse events. Moreover, CTO-ischaemia (OR = 5.466; 95% CI 1.015-29.420; P = 0.048), non-CTO ischaemia (OR = 29.174; 95% CI 3.245-262.322; P = 0.003), mixed-ischaemia (OR = 7.130, 95% CI 1.257-40.445; P = 0.027) were all independent predictors for outcome. CONCLUSION: Ischaemia demonstrated on MPI, especially CTO-ischaemia were independent predictors for the adverse events. SPECT MPI can aid to identify patients at risk of adverse events, who may benefit from subsequent CTO percutaneous coronary intervention.

8.
Nat Nanotechnol ; 16(11): 1260-1270, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34594005

RESUMO

Nutritional metal ions play critical roles in many important immune processes. Hence, the effective modulation of metal ions may open up new forms of immunotherapy, termed as metalloimmunotherapy. Here, we demonstrate a prototype of cancer metalloimmunotherapy using cyclic dinucleotide (CDN) stimulator of interferon genes (STING) agonists and Mn2+. We screened various metal ions and discovered specific metal ions augmented STING agonist activity, wherein Mn2+ promoted a 12- to 77-fold potentiation effect across the prevalent human STING haplotypes. Notably, Mn2+ coordinated with CDN STING agonists to self-assemble into a nanoparticle (CDN-Mn2+ particle, CMP) that effectively delivered STING agonists to immune cells. The CMP, administered either by local intratumoural or systemic intravenous injection, initiated robust anti-tumour immunity, achieving remarkable therapeutic efficacy with minute doses of STING agonists in multiple murine tumour models. Overall, the CMP offers a new platform for local and systemic cancer treatments, and this work underscores the great potential of coordination nanomedicine for metalloimmunotherapy.

9.
Mitochondrial DNA B Resour ; 6(10): 3064-3066, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595338

RESUMO

Smilax glabra is a perennial woody scandent shrub, of which the dried aerial tuber has been used as Chinese medicine. Here, we sequenced S. glabra and assembled its complete chloroplast (cp) genome. The genome is 157,889 bp in length and has a typical quadripartite structure. We annotated 131 genes, of which 84 were protein-coding genes, 37 were tRNAs and 8 were rRNA genes. Phylogenetic analysis of this genome with 26 representatives Liliales fully resolved S. glabra in a clade with S. china. The phylogenetic tree we constructed is largely consistent with recently published phylogenetic trees using both complete chloroplast genomes and marker gene sequences.

10.
Oncogene ; 40(49): 6680-6691, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34645979

RESUMO

Colorectal carcinoma (CRC) is the second most deadly cancer worldwide. Therapies that take advantage of DNA repair defects have been explored in various tumors but not yet systematically in CRC. Here, we found that Diphosphoinositol Pentakisphosphate Kinase 2 (PPIP5K2), an inositol pyrophosphate kinase, was highly expressed in CRC and associated with a poor prognosis of CRC patients. In vitro and in vivo functional studies demonstrated that PPIP5K2 could promote the proliferation and migration ability of CRC cells independent of its inositol pyrophosphate kinase activity. Mechanically, S1006 dephosphorylation of PPIP5K2 could accelerate its dissociation with 14-3-3 in the cytoplasm, resulting in more nuclear distribution. Moreover, DNA damage treatments such as doxorubicin (DOX) or irradiation (IR) could induce nuclear translocation of PPIP5K2, which subsequently promoted homologous recombination (HR) repair by binding and recruiting RPA70 to the DNA damage site as a novel scaffold protein. Importantly, we verified that S1006 dephosphorylation of PPIP5K2 could significantly enhance the DNA repair ability of CRC cells through a series of DNA repair phenotype assays. In conclusion, PPIP5K2 is critical for enhancing the survival of CRC cells via facilitating DNA HR repair. Our findings revealed an unrecognized biological function and mechanism model of PPIP5K2 dependent on S1006 phosphorylation and provided a potential therapeutic target for CRC patients.

11.
Front Microbiol ; 12: 738226, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34690980

RESUMO

The intestinal microbial community composition of different bee species typically has host specificity, yet little is known about the underlying formation mechanism. There are signs that dietary habits vary in different bee species, suggesting that there may be close relationships between dietary habits and intestinal microorganisms. We explored this hypothesis by comparing the dietary habits and gut microbiota of two common bee species (Apis mellifera L. and Apis cerana cerana) in China. Bee bread and midgut samples from wild and laboratory-reared bees were collected, and the differences in intestinal microbial community composition and growth and development before and after the change in dietary habits of different bee species were compared. We found that the two sympatric species had different dietary specializations and similar metagenomic diversities. The microbiota composition differed between the two species. Moreover, we revealed that changes in native dietary habits destroyed the intestinal microbiota community composition, negatively affecting the growth and development of honeybees.

12.
Sci China Mater ; : 1-9, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34692172

RESUMO

Perusing multimode luminescent materials capable of being activated by diverse excitation sources and realizing multi-responsive emission in a single system remains a challenge. Herein, we utilize a heterovalent substituting strategy to realize multimode deep-ultraviolet (UV) emission in the defect-rich host Li2CaGeO4 (LCGO). Specifically, the Pr3+ substitution in LCGO is beneficial to activating defect site reconstruction including the generation of cation defects and the decrease of oxygen vacancies. Regulation of different traps in LCGO:Pr3+ presents persistent luminescence and photo-stimulated luminescence in a synergetic fashion. Moreover, the up-conversion luminescence appears with the aid of the 4f discrete energy levels of Pr3+ ions, wherein incident visible light is partially converted into germicidal deep-UV radiation. The multi-responsive character enables LCGO:Pr3+ to response to convenient light sources including X-ray tube, standard UV lamps, blue and near-infrared lasers. Thus, a dual-mode optical conversion strategy for inactivating bacteria is fabricated, and this multi-responsive deep-UV emitter offers new insights into developing UV light sources for sterilization applications. Heterovalent substituting in trap-mediated host lattice also provides a methodological basis for the construction of multi-mode luminescent materials.

13.
Natl Sci Rev ; 8(4): nwab006, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34676097

RESUMO

After a short recovery period, COVID-19 reinfections could occur in convalescent patients, even those with measurable levels of neutralizing antibodies. Effective vaccinations and protective public health measures are recommended for the convalescent COVID-19 patients.

14.
ACS Catal ; 11(17): 11049-11058, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34513203

RESUMO

By photodeposition of CrOx on SrTiO3-based semiconductors doped with aliovalent Mg(II) and functionalized with Ni/NiOx catalytic nanoparticles (economically significantly more viable than commonly used Rh catalysts), an increase in apparent quantum yield (AQYs) from ∼10 to 26% in overall water splitting was obtained. More importantly, deposition of CrOx also significantly enhances the stability of Ni/NiO nanoparticles in the production of hydrogen, allowing sustained operation, even in intermittent cycles of illumination. In situ elemental analysis of the water constituents during or after photocatalysis by inductively coupled plasma mass spectrometry/optical emission spectrometry shows that after CrOx deposition, dissolution of Ni ions from Ni/NiOx-Mg:SrTiO3 is significantly suppressed, in agreement with the stabilizing effect observed, when both Mg dopant and CrOx are present. State-of-the-art electron microscopy and energy-dispersive X-ray spectroscopy (EDX) and electron energy-loss spectroscopy (EELS) analyses demonstrate that upon preparation, CrOx is photodeposited in the vicinity of several, but not all, Ni/NiOx particles. This implies the formation of a Ni-Cr mixed metal oxide, which is highly effective in water reduction. Inhomogeneities in the interfacial contact, evident from differences in contact angles between Ni/NiOx particles and the Mg:SrTiO3 semiconductor, likely affect the probability of reduction of Cr(VI) species during synthesis by photodeposition, explaining the observed inhomogeneity in the spatial CrOx distribution. Furthermore, by comparison with undoped SrTiO3, Mg-doping appears essential to provide such favorable interfacial contact and to establish the beneficial effect of CrOx. This study suggests that the performance of semiconductors can be significantly improved if inhomogeneities in interfacial contact between semiconductors and highly effective catalytic nanoparticles can be resolved by (surface) doping and improved synthesis protocols.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34546919

RESUMO

This paper introduces versatile filters to construct efficient convolutional neural networks that are widely used in various visual recognition tasks. Considering the demands of efficient deep learning techniques running on cost-effective hardware, a number of methods have been developed to learn compact neural networks. Most of these works aim to slim down filters in different ways, e.g. investigating small, sparse or quantized filters. In contrast, we treat filters from an additive perspective. A series of secondary filters can be derived from a primary filter with the help of binary masks. These secondary filters all inherit in the primary filter without occupying more storage, but once been unfolded in computation they could significantly enhance the capability of the filter by integrating information extracted from different receptive fields. Besides spatial versatile filters, we additionally investigate versatile filters from the channel perspective. Binary masks can be further customized for different primary filters under orthogonal constraints. We conduct theoretical analysis on network complexity and an efficient convolution scheme is introduced. Experimental results on benchmark datasets and neural networks demonstrate that our versatile filters are able to achieve comparable accuracy as that of original filters, but require less memory and computation cost.

16.
Front Immunol ; 12: 735125, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34567001

RESUMO

Background: The global outbreak of coronavirus disease 2019 (COVID-19) has turned into a worldwide public health crisis and caused more than 100,000,000 severe cases. Progressive lymphopenia, especially in T cells, was a prominent clinical feature of severe COVID-19. Activated HLA-DR+CD38+ CD8+ T cells were enriched over a prolonged period from the lymphopenia patients who died from Ebola and influenza infection and in severe patients infected with SARS-CoV-2. However, the CD38+HLA-DR+ CD8+ T population was reported to play contradictory roles in SARS-CoV-2 infection. Methods: A total of 42 COVID-19 patients, including 32 mild or moderate and 10 severe or critical cases, who received care at Beijing Ditan Hospital were recruited into this retrospective study. Blood samples were first collected within 3 days of the hospital admission and once every 3-7 days during hospitalization. The longitudinal flow cytometric data were examined during hospitalization. Moreover, we evaluated serum levels of 45 cytokines/chemokines/growth factors and 14 soluble checkpoints using Luminex multiplex assay longitudinally. Results: We revealed that the HLA-DR+CD38+ CD8+ T population was heterogeneous, and could be divided into two subsets with distinct characteristics: HLA-DR+CD38dim and HLA-DR+CD38hi. We observed a persistent accumulation of HLA-DR+CD38hi CD8+ T cells in severe COVID-19 patients. These HLA-DR+CD38hi CD8+ T cells were in a state of overactivation and consequent dysregulation manifested by expression of multiple inhibitory and stimulatory checkpoints, higher apoptotic sensitivity, impaired killing potential, and more exhausted transcriptional regulation compared to HLA-DR+CD38dim CD8+ T cells. Moreover, the clinical and laboratory data supported that only HLA-DR+CD38hi CD8+ T cells were associated with systemic inflammation, tissue injury, and immune disorders of severe COVID-19 patients. Conclusions: Our findings indicated that HLA-DR+CD38hi CD8+ T cells were correlated with disease severity of COVID-19 rather than HLA-DR+CD38dim population.


Assuntos
Linfócitos T CD8-Positivos/imunologia , COVID-19/imunologia , Doenças do Sistema Imunitário/imunologia , SARS-CoV-2 , Adulto , Idoso , Antígenos CD8/imunologia , Citocinas/imunologia , Feminino , Antígenos HLA-DR/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Índice de Gravidade de Doença , Adulto Jovem
17.
Microsyst Nanoeng ; 7: 7, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34567725

RESUMO

Ammonia synthesis using low-power consumption and eco-friendly methods has attracted increasing attention. Here, based on the Tesla turbine triboelectric nanogenerator (TENG), we designed a simple and effective self-powered ammonia synthesis system by N2 discharge. Under the driving of the simulated waste gas, the Tesla turbine TENG showed high rotation speed and high output. In addition, the performance of two Tesla turbine TENGs with different gas path connections was systematically investigated and discussed. A controllable series-parallel connection with the control of gas supply time was also proposed. Taking advantage of the intrinsic high voltage, corona discharge in a N2 atmosphere was simply realized by a Tesla turbine TENG. With the flow of N2, the generated high-energy plasma can immediately react with water molecules to directly produce ammonia. The self-powered system achieved a yield of 2.14 µg h-1 (0.126 µmol h-1) under ambient conditions, showing great potential for large-scale synthesis.

18.
Analyst ; 146(20): 6202-6210, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34519726

RESUMO

Chloride ion (Cl-), the most common anion in animal brain, has been verified to play a vital role in maintaining normal physiological processes. Thus, development of a reliable platform to determine Cl- is of great significance for brain research involving Cl-. In this work, a ratiometric electrochemical microsensor (REM) for the in vivo measurement of cerebral Cl- was designed. To prepare REM, uniform Ag nanoparticles (Ag NPs) with nano-level sizes were synthesized via an adsorption-reduction process, which served as selective recognition elements for Cl- determination, while methylene blue (MB) was absorbed and acted as an inner reference unit to avoid the environmental interference of complicated brain systems. As a result, this developed REM exhibited high sensitivity and selectivity, as well as good stability, reproducibility and anti-biofouling. This reliable approach was established to monitor Cl- in mouse brain.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Animais , Cloretos , Técnicas Eletroquímicas , Camundongos , Reprodutibilidade dos Testes , Prata
19.
ACS Appl Mater Interfaces ; 13(36): 43063-43074, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34473488

RESUMO

To develop next-generation electronic devices, novel semiconductive materials are urgently required. The transition metal dichalcogenides (TMDs) hold the promise of next generation of semiconductor materials for emerging electronic applications. As a member of the group-10 TMDs, PdS2 has a notable layer-number-dependent band structure and tremendously high carrier mobility at room temperature. Here, we demonstrate the experimental realization of centimeter-scale synthesis of the few-layer PdS2 by the combination of physical vapor deposition (PVD) and chemical vapor deposition (CVD) methods. For the first time, the optical anisotropic properties of the few-layer PdS2 were investigated through angle-resolved polarized Raman spectroscopy. Also, the evolution of Raman spectra was studied depending on the temperature in the range of 12-300 K. To further understand the electronic properties of the few-layer PdS2, the field-effect transistor (FET) devices were fabricated and investigated. The electronic measurements of such FET devices reveal that the PdS2 materials exhibit a tunable ambipolar transport mechanism with field-effect mobility of up to ∼388 cm2 V-1 s-1 and the on/off ratio of ∼800, which were not reported before in the literature. To well understand the experimental results, the electronic structure of PdS2 was determined using density functional theory (DFT) calculations. These excellent physical properties are very helpful in developing high-performance opto-electronic applications.

20.
Mol Ecol Resour ; 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34486812

RESUMO

The 15 species of small carnivorous marsupials that comprise the genus Antechinus exhibit semelparity, a rare life-history strategy in mammals where synchronized death occurs after one breeding season. Antechinus males, but not females, age rapidly (demonstrate organismal senescence) during the breeding season and show promise as new animal models of ageing. Some antechinus species are also threatened or endangered. Here, we report a chromosome-level genome of a male yellow-footed antechinus Antechinus flavipes. The genome assembly has a total length of 3.2 Gb with a contig N50 of 51.8 Mb and a scaffold N50 of 636.7 Mb. We anchored and oriented 99.7% of the assembly on seven pseudochromosomes and found that repetitive DNA sequences occupy 51.8% of the genome. Draft genome assemblies of three related species in the subfamily Phascogalinae, two additional antechinus species (Antechinus argentus and A. arktos) and the iteroparous sister species Murexia melanurus, were also generated. Preliminary demographic analysis supports the hypothesis that climate change during the Pleistocene isolated species in Phascogalinae and shaped their population size. A transcriptomic profile across the A. flavipes breeding season allowed us to identify genes associated with aspects of the male die-off. The chromosome-level A. flavipes genome provides a steppingstone to understanding an enigmatic life-history strategy and a resource to assist the conservation of antechinuses.

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