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1.
World Neurosurg ; 2020 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-32294565

RESUMO

OBJECTIVE: To investigate the effect of partial facetectomy on lumbar stability using percutaneous endoscopy. METHODS: Five male adult volunteers with no history of lumbar disease participated in the study. Based on computed tomography data, a three-dimensional model of the L3-S1 segment was created using the Mimics l5.0 and Ansys 13.0 software. The use of an 8.5-mm-diameter ring saw was simulated to cut through 5 different needle insertion points (IPs) commonly used in the clinic on the left-side facet joint (FJ) of L5 to perform facetectomy. The first to third IPs were on the apex of the superior FJ, the midpoint of the ventral side of the superior FJ, and the lowest point of the ventral side of the superior FJ. The fourth and fifth IPs represented the positions of the second and third IPs (8.5 mm/2) after the radius of the ring saw was translated to the dorsal side of the superior FJ. Physiologic load was applied to the human models. The pressure on the left and right FJ of the L5 vertebra, the pressure on the L4-5 intervertebral disc, and the range of motion of the lumbar spine were recorded when normal flexion and extension and lateral flexion and rotation of the lumbar spine model after facetectomy were simulated. RESULTS: Compared with the intact group, the second IP, maximum pressure on the L4-5 intervertebral disc after facetectomy was not significantly different under any condition (P > 0.05). The maximum pressure on the left FJ of L5 showed significant differences during right rotation of the lumbar spine (P < 0.05). The pressure on the right FJ of L5 was significantly different during left rotation of the lumbar spine (P < 0.05). The range of motion of the lumbar spine was not significantly different under any condition (P > 0.05). CONCLUSIONS: The second IP at the midpoint of the ventral side of the superior FJ showed minimal effect on lumbar spine biomechanics compared with all the other IPs during percutaneous transforaminal facetectomy. Thus, it can be considered as the most suitable IP for facetectomy.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32302508

RESUMO

Background: Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity. It was determined that circular RNAs were related to the development and progression of various cancers, including OSCC. The purpose of our study was to figure out the role and potential mechanism of hsa_circ_0072387 in OSCC progression. Materials and Methods: Thirty-five patients with OSCC were involved in this study. Real-time quantitative polymerase chain reaction was used to evaluate the expression levels of hsa_circ_0072387 and microRNA (miR)-503-5p. Cell proliferation, migration, and invasion abilities were assessed by Cell Counting Kit-8 (CCK-8) and Transwell assays, respectively. The abundance of cell proliferation marker Ki-67, epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin, and vimentin was analyzed by western blot assay. Glycolysis was evaluated using commercial kits. The interaction between hsa_circ_0072387 and miR-503-5p was confirmed by bioinformatics analysis, RNA immunoprecipitation (RIP) assay, and dual-luciferase reporter assay. Results: hsa_circ_0072387 expression was significantly downregulated, and miR-503-5p was upregulated in OSCC cells and tissues. Gain of hsa_circ_0072387 or knockdown of miR-503-5p suppressed the cell proliferation, migration and invasion, EMT, and glycolysis in OSCC SCC-4 and HSC-3 cells. hsa_circ_0072387 targeted miR-503-5p and inversely regulated miR-503-5p expression. Moreover, upregulation of miR-503-5p could partially revert the tumor-suppressive effects of hsa_circ_0072387 on OSCC cells. Conclusion: hsa_circ_0072387 inhibited OSCC progression by downregulating miR-503-5p, explicating that hsa_circ_0072387 could function as a novel potential therapeutic target for OSCC.

3.
Colloids Surf B Biointerfaces ; 188: 110797, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31958621

RESUMO

A novel non-enzymatic glucose sensor was established by galvanic replacement synthesis of Pd nanosheets (PdNS) on Cu/Cu2O nanocomposites using fluorine-doped tin oxide (FTO) substrates. To prepare PdNS-Cu/Cu2O/FTO electrode, Cu/Cu2O nanocomposites were firstly deposited on FTO electrode through potential oscillating, and then Pd nanosheets were synthesized on Cu/Cu2O nanocomposites by galvanic replacement reduction. X-ray diffraction, scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy were applied to investigate the morphology and composition of PdNS-Cu/Cu2O nanohybrids. The synthesized PdNS-Cu/Cu2O exhibited great electrochemical activity toward glucose oxidation. Under optimized conditions, the proposed glucose sensor displayed a wide linear range from 0.5 µM to 2600 µM with detection limit of 0.1 µM. In addition, the proposed electrode was used to determine glucose in true blood specimen, indicating potential application for routine glucose analysis.

4.
Orthop Surg ; 12(1): 233-240, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31958880

RESUMO

OBJECTIVES: To determine whether the rates of postoperative complications, rate of readmission, cumulative transfusion volume, and length of stay (LOS) differ between simultaneous total hip arthroplasty (THA) and staged bilateral THA and to assess whether the length of the interval between staged procedures influences surgery outcome. METHODS: This was a retrospective cohort study comparing the rate of postoperative complications, readmission, cumulative transfusion volume, and LOS between simultaneous THA and staged bilateral THA in our hospital's registration database. The inclusion criteria is listed as follows: patients who underwent bilateral primary THA between January 2011 and January 2015 with minimum 3-month follow-up; simultaneous bilateral THA; staged bilateral THA; postoperative complications, readmission, cumulative transfusion volume, length of stay of the patients and the influence of the interval between stages of bilateral THA on the outcome above; and retrospective cohort study. Finally, a total of 1145 patients, including simultaneous bilateral THA in 863 patients (1726 hips) and staged bilateral THA in 282 patients (564 hips), were eligible for the present study. The patients were divided into three groups according to the interval time (≤30 days, 30-90 days, >90 days) between the two stages of bilateral THA and we compared postoperative complications, readmission rates, cumulative transfusion volume, and LOS for the three groups. All patients' medical records and outpatient notes were reviewed to extract preoperative data, perioperative complications, readmission, cumulative transfusion, and LOS. Preoperative information included patients' age, sex, diagnosis, body mass index, and American Society of Anesthesiologists (ASA) classification. Perioperative complications were sorted into two groups: (i) medical complications included cardiovascular, pulmonary, neurological, digestive, and urologic system complications, along with other miscellaneous issues; and (ii) surgical complications included dislocation, superficial wound infection, hematoma, deep periprosthetic joint infection, and nerve palsy. Patients who failed to come back to visit our hospital in the postoperative 3 months were followed up by telephone, at which point we inquired about any postoperative complications and readmission. RESULTS: Simultaneous THA was performed more often in younger men, and patients in the simultaneous group had fewer major medical complications (excluding venous thromboembolism), fewer surgical complications, and shorter hospital stays; however, patients in the simultaneous group were likelier to have a higher transfusion rate than patients in the staged group. Among patients in the staged group, there were no differences for differing time intervals, except that patients with a between-stage interval of ≤30 days required more blood transfusions. CONCLUSION: With careful patient assessment and selection, simultaneous bilateral THA is a safe procedure, and has lower rates of surgical and major medical complications than staged bilateral THA.

5.
J Ethnopharmacol ; 250: 112483, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31843573

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: San-Huang-Tang (ST), a classic prescription, has been clinically used to cure diabetes and diabetes-associated metabolic disorders. Established studies have reported that ST can alleviate inflammation, obesity, hyperglycemia and insulin resistance. AIM OF THE STUDY: To the best of our knowledge, here, we reported for the first time the underlying mechanistic therapeutic efficacy of the ST against nonalcoholic fatty liver disease (NAFLD) in high-fat induced obese and galr1-deficient diabetic mice. MATERIALS AND METHODS: The obese and galr1-deficient mice were treated with ST at a dose of 10 g/kg every day for three weeks. Then food intake, body weight and insulin resistance indexes were measured. Western blotting, qRT-PCR, and plasma biochemical analyses were applied. RESULTS: ST reduced food intake, body weight, blood glucose level and insulin resistance, improved glucose tolerance in obese and galr1-deficient mice. Mechanistically, we confirmed that ST protected against NAFLD through activation of PGC-1α and its downstream signaling pathways as shown by the attenuated hepatic adipogenesis and lipid accumulation, increased hepatic fatty acid oxidation, regulated plasma lipid parameters, and increased energy expenditure and metabolic function in fat and muscle. CONCLUSIONS: Reduction in food intake produced by ST may contribute to the observed metabolic effects. Our findings strongly suggest that ST might be a potential novel therapeutic drug against obesity/diabetes-induced NAFLD and other metabolic disorders.

6.
J Cancer ; 10(26): 6673-6680, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31777596

RESUMO

Emerging evidence revealed the critical role of systematic inflammation in pancreatic ductal adenocarcinoma (PDAC). In the present study, we reviewed the records of 279 patients with advanced PDAC. Among them, 147 cases were used as the training cohort and another 132 as the validation cohort. In the training cohort, distant metastasis, carbohydrate antigen 19-9 (CA19-9), Glasgow prognostic score (GPS), neutrophil-to-lymphocyte ratio (NLR), and lymphocyte-to-monocyte ratio (LMR) were independent prognostic factors in Cox regression. A nomogram based on these factors was generated to predict median survival time and survival probabilities at 6, 12, and 18 months. The nomogram showed a better discriminatory ability than the American Joint Committee on Cancer (AJCC) TNM staging (C-index: 0.727 vs. 0.610). In the validation cohort, a nomogram composed of the same variables also showed a high discriminatory ability (C-index: 0.784). In the low-risk group with a nomogram total point (NTP) value of more than 175, patients receiving combination therapy showed better prognosis than those receiving monotherapy (P=0.015). In conclusion, the nomogram based on inflammatory biomarkers can serve as useful prognostic tool for advanced PDAC. In addition, patients with high NTP can greater benefit from combination chemotherapy than monotherapy.

7.
Cell Tissue Bank ; 20(4): 557-568, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31583486

RESUMO

The purpose of this paper is to analyze the properties of porcine cartilage type II collagen scaffolds crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy-succinamide (EDC/NHS) under different conditions. The porous EDC/NHS-crosslinked scaffolds were obtained through a two-step freeze-drying process. To determine the optimal crosslinking condition, we used different solvents and various crosslinking temperatures to prepare the scaffolds. Three crosslinking solutions were prepared with different solvents, photographs were taken with a flash in the darkroom, and light transmission was observed. Type II collagen was crosslinked on a horizontal shaker at a speed of 60 r/min according to the above grouping conditions, and then the structural change of the scaffold in each group was observed. To investigate the swelling ratio and the in vitro degradation of the collagen scaffold, tests were also carried out by immersion of the scaffolds in a PBS solution and digestion in type II collagenase, respectively. The influence of the scaffolds on the proliferation of chondrocytes was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The morphology of the crosslinked scaffolds cocultured with chondrocytes was characterized by a scanning electron microscope. The results proved that 75% alcohol and a crosslinking temperature of 37 °C are recommended. Collagen fibrils are more densely packed after crosslinking with EDC/NHS and have a more uniform structure than that of noncrosslinked ones. The EDC-crosslinked scaffolds possessed excellent mechanical property and biocompatibility.

9.
Biochemistry ; 58(14): 1931-1941, 2019 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-30888187

RESUMO

The chaperone-usher secretion pathway is a conserved bacterial protein secretion system dedicated to the biogenesis of adhesive fibers. Usher, a multidomain-containing outer membrane protein, plays a central role in this process by acting as a molecular machine that recruits different chaperone-subunit complexes, catalyzes subunit polymerization, and forms a channel for secretion of the assembled subunits. While recent crystal structural studies have greatly advanced our understanding of the structure and function of ushers, the overall architecture of the full-length apo-usher, the molecular events that dictate conformational changes in usher during pilus biogenesis, and its activation by the specific chaperone-adhesin complex remain largely elusive. Using single-molecule fluorescence resonance energy transfer studies, we found that the substrate-free usher FimD (apo-FimD) adopts a contracted conformation that is distinct from its substrate-bound states; both the N-terminal domain (NTD) and the C-terminal domain (CTD) of apo-FimD are highly dynamic, and FimD coordinates its domain conformational changes via intramolecular domain conformation signaling. By combining these studies with in vitro photo-cross-linking studies, we further show that only the chaperone-bound adhesin (FimC:FimH) can be transferred to the CTD, dislocates the plug domain, and triggers conformational changes in the remaining FimD domains. Taken together, these studies delineate an overall architecture of the full-length apo-FimD, provide detailed mechanic insight into the activation of apo-FimD, and explain why FimD could adjust its conformational states to perform multiple functions in each cycle of pilus subunit addition and ensure that pilus assembly proceeds progressively in a cellular energy-free environment.


Assuntos
Proteínas de Escherichia coli/química , Proteínas de Fímbrias/química , Transferência Ressonante de Energia de Fluorescência/métodos , Chaperonas Moleculares/química , Conformação Proteica , Domínios Proteicos , Sistemas de Secreção Bacterianos/genética , Sistemas de Secreção Bacterianos/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteínas de Fímbrias/genética , Proteínas de Fímbrias/metabolismo , Polarização de Fluorescência , Cinética , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Mutação , Ligação Proteica , Transdução de Sinais/genética
10.
Chin J Traumatol ; 22(1): 24-28, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30744941

RESUMO

PURPOSE: To evaluate the clinical outcome in patients who received anterior cruciate ligament (ACL) reconstruction via anteromedial portal with or without posterior wall blowout. METHODS: Twenty patients with ruptured ACL, who have received ACL reconstruction via anteromedial portal between Apr 2012 and Oct 2013 were enrolled. According to the conditions of posterior wall, the patients were divided into 2 groups: posterior wall blowout group (10 patients) and posterior wall intact group (10 patients). The median follow up time were 63 (range 19-75) months and 60.5 (range 25-64) months in the 2 groups respectively. The clinical outcome was evaluated by knee joint physical examination, magnetic resonance imaging (MRI), the International Knee Documentation Committee (IKDC) 2000 subjective score, Lysholm score, Tenger score, difference of thigh circumference, KT-2000 and Biodex isokinetic dynamometer system. RESULTS: No significant differences were found in terms of the IKDC score, Lysholm score, Tegner score, Lachman test positive rate or Pivot Shift test positive rate between the two groups. In KT-2000 and Biodex isokinetic dynamometer tests, the difference of muscle strength between affected knees and unaffected knees in posterior wall blowout group was not significant less than that of posterior wall intact group (p > 0.05). In addition, there is no statistical difference between the two groups in signal/noise quotient (SNQ) of the graft (p > 0.05) in post operative MRI. CONCLUSION: Blowout of posterior wall in ACL reconstruction via anteromedial portal does not affect the clinical outcome as long as reliable fixation is taken intraoperatively.


Assuntos
Lesões do Ligamento Cruzado Anterior/cirurgia , Ligamento Cruzado Anterior/cirurgia , Procedimentos Ortopédicos/métodos , Procedimentos Cirúrgicos Reconstrutivos/métodos , Adolescente , Adulto , Ligamento Cruzado Anterior/patologia , Lesões do Ligamento Cruzado Anterior/diagnóstico , Lesões do Ligamento Cruzado Anterior/patologia , Lesões do Ligamento Cruzado Anterior/reabilitação , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
11.
Analyst ; 144(2): 685-690, 2019 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-30516176

RESUMO

In this work, copper/cuprous oxide (Cu/Cu2O) nanocomposites were electrodeposited on a fluorine doped tin oxide (FTO) glass substrate for sensitive determination of hydrogen peroxide (H2O2). The Cu/Cu2O nanocomposites were synthesized on FTO at a constant current density of -0.4 mA cm-2 in 0.3 M CuSO4 (pH 9.5) under magnetic agitation. The composition and morphology of Cu/Cu2O nanocomposites were characterized by X-ray diffraction, scanning electron microscopy, energy-dispersive spectroscopy and X-ray photoelectron spectroscopy. Taking advantage of the synergistic effects of Cu and Cu2O, the fabricated Cu/Cu2O/FTO electrode showed excellent electrocatalytic activity towards the oxidation of H2O2. The electrocatalytic performance of Cu/Cu2O/FTO was evaluated by linear sweep voltammetry and amperometry. Under optimized conditions, the developed sensor exhibited a wide linear range of 0.2-2000 µM for the determination of H2O2 with a detection limit of 0.04 µM (S/N = 3). In addition, the proposed H2O2 sensor was successfully applied for the determination of H2O2 in milk samples, indicating that the electrodeposited Cu/Cu2O nanocomposites are promising nanomaterials for electrochemical sensors.

12.
Hum Brain Mapp ; 40(4): 1049-1061, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30593684

RESUMO

The processes involved in value evaluation and self-control are critical when making behavioral choices. However, the evidence linking these two types of processes to behavioral choices in intertemporal decision-making remains elusive. As the ventromedial prefrontal cortex (vmPFC), striatum, and dorsolateral prefrontal cortex (dlPFC) have been associated with these two processes, we focused on these three regions. We employed functional magnetic resonance imaging during a delayed discounting task (DDT) using a relatively large sample size, three independent samples. We evaluated how much information about a specific choice could be decoded from local patterns in each brain area using multivoxel pattern analysis (MVPA). To investigate the relationship between the dlPFC and vmPFC/striatum regions, we performed a psychophysiological interaction (PPI) analysis. In Experiment I, we found that the vmPFC and dlPFC, but not the striatum, could determine choices in healthy participants. Furthermore, we found that the dlPFC showed significant functional connectivity with the vmPFC, but not the striatum, when making decisions. These results could be replicated in Experiment II with an independent sample of healthy participants. In Experiment III, the choice-decoding accuracy in the vmPFC and dlPFC was lower in patients with addiction (smokers and participants with Internet gaming disorder) than in healthy participants, and decoding accuracy in the dlPFC was related to impulsivity in addicts. Taken together, our findings may provide neural evidence supporting the hypothesis that value evaluation and self-control processes both guide the intertemporal choices, and might provide potential neural targets for the diagnosis and treatment of impulsivity-related brain disorders.

13.
Anal Chim Acta ; 1042: 20-28, 2018 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-30428984

RESUMO

Bimetallic Au-Ag/Co3O4 nanofibers (Au-Ag/Co3O4NFs) were synthesized by an electrospinning method and calcination treatment. Due to the admirable synergic effect of electrocatalytic ability of Au and Ag, and the large surface-to-volume ratio of Co3O4 nanofibers caused by their rough surface, the synthesized Au-Ag/Co3O4NFs were applied to a sensing platform for hydrogen peroxide oxidation. Using electrochemical techniques, the novel sensing platform based on Au-Ag/Co3O4NFs shows a wide linear concentration range with high detection sensitivity of 1241.1 µA mM-1 cm-2. This sensitive sensor was also applied to determine H2O2 from human breast cancer cells, indicating its great potential application in physiology and pathology.


Assuntos
Neoplasias da Mama/metabolismo , Cobalto/química , Ouro/química , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/metabolismo , Nanofibras/química , Óxidos/química , Prata/química , Neoplasias da Mama/patologia , Feminino , Humanos , Tamanho da Partícula , Propriedades de Superfície
14.
Acta Biochim Pol ; 65(3): 425-429, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30212594

RESUMO

Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. However, it is unknown whether MinD protein regulates the same plasmid-dependent genes. We replaced pgp5 gene in the shuttle vector pGFP::CM with minD gene of C. trachomatis (CT0582) or C. muridarum (TC0871). The recombinant plasmid was transformed into plasmid-free organisms-CMUT3 and qRT-PCR was used to detect the transcription level of plasmid-encoded and -dependent genes in these pgp5 deficient organisms. As a readout, GlgA, one of the plasmid-regulated gene products was detected by immunofluorescence assay. After recombination, transformation and plaque purification, the stable transformants CT0582R and TC0871R were generated. In these transformants, the plasmid-dependent genes were up-regulated, alike in the pgp5 premature stop mutant and pgp5 replacement with mCherry mutant. GlgA protein level was also increased in all pgp5 mutants, including CT0582R and TC0871R. Thus, our study showed that genomic MinD protein had different function than Pgp5, which was useful for further understanding the chlamydiae.


Assuntos
Proteínas de Bactérias/genética , Chlamydia muridarum/genética , Chlamydia trachomatis/genética , Genes Bacterianos , Plasmídeos/genética , Técnica Indireta de Fluorescência para Anticorpo , Vetores Genéticos , Células HeLa , Humanos , Mutação , Reação em Cadeia da Polimerase em Tempo Real
15.
Biosens Bioelectron ; 122: 239-246, 2018 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-30267982

RESUMO

Biosensing and detecting the rare circulating tumor cells (CTCs) in complex blood samples are a great challenge but necessary for cancer metastasis prevention. Here we show a novel highly-sensitive biosensing system for detecting CTCs in whole blood. The system is composed of Her2-coated immunomagnetic beads and an anti-EpCAM aptamer assembled pseudo-DNA nanocatenane (PDN) for dual targeting and separating CTCs, in conjunction with the rolling circle amplification (RCA) and molecular beacon (MB) system for CTCs signal amplification. The Her-2-coated beads separated CTCs from blood after their elution from a magnetic column. The unique PDN, which is a tailor-designed self-assembly of three circular DNAs that are inter-locked with independent and non-interfered templates for periodic RCA process, binds EpCAM-rich CTCs. In the presence of the RCA primer, phi29 DNA polymerase and MB, the system collaboratively generated the amplified fluorescent signals for highly-sensitive detection of CTCs. Through this system, we achieved the limit of detection less than 10 CTCs/mL blood, and quantified the number of CTCs in patient blood, which is proportional to the patient cancer status. Our technique is highly-sensitive, practicable and convenient enough for clinical detection of breast CTCs.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Neoplasias da Mama/sangue , Separação Imunomagnética/métodos , Células Neoplásicas Circulantes/patologia , Anticorpos Imobilizados/química , Neoplasias da Mama/diagnóstico , Linhagem Celular Tumoral , Molécula de Adesão da Célula Epitelial/análise , Feminino , Humanos , Células Neoplásicas Circulantes/química , Técnicas de Amplificação de Ácido Nucleico/métodos , Receptor ErbB-2/análise
16.
Int J Biol Sci ; 14(10): 1333-1342, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30123080

RESUMO

Background & aims: VEGFR-3 has been shown of great significance in lymph node metastasis and some malignancies, however, its expression in tumors and impact on outcome of intrahepatic cholangiocarcinoma (iCCA) remains unknown. The aim of this study was to assess the role of VEGFR-3 positive tumors for prognosis of iCCA and tumor-associated lymphangiogenesis. Methods: Clinicopathological features, prognostic factors and survival rate were analyzed to evaluate the influence of VEGFR-3 positive expression on prognosis of iCCA. In addition, tumor-associated lymphangiogenesis quantified as micro-lymphatic vessel density (MLVD) was assessed to explore the correlation between VEGFR-3 expression and lymph node metastasis for iCCA. Results: Patients with VEGFR-3 positive tumors had increased lymph node metastasis (p=0.025) and were more likely to suffer from tumor recurrence compared with VEGFR-3 negative tumors (p<0.001). VEGFR-3 expression in tumors was identified as an independent prognostic factor for both overall and recurrence-free survival in surgical resected patients with iCCA. In addition, higher MLVD was significantly associated with VEGFR-3 positive expression in tumors (p<0.001), which facilitate lymph node metastasis and significantly worse survival rates. Conclusions: Our study reveals that VEGFR-3 positive expression in tumors represents an independent prognostic factor for both overall and recurrence-free survival in hepatic resected patients with iCCA. VEGFR-3 positive tumors favor lymph node metastasis, tumor recurrence and worse outcomes through tumor-associated lymphangiogenesis.


Assuntos
Colangiocarcinoma/metabolismo , Colangiocarcinoma/patologia , Receptor 3 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Colangiocarcinoma/genética , Feminino , Humanos , Imuno-Histoquímica , Linfangiogênese/fisiologia , Metástase Linfática/genética , Metástase Linfática/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Receptor 3 de Fatores de Crescimento do Endotélio Vascular/genética
17.
Med Sci Monit ; 24: 5159-5167, 2018 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-30043770

RESUMO

BACKGROUND The anti-microbial protein cathelicidin LL-37 plays an important role in the pathogenesis of psoriasis by inducing inflammation. Our previous study showed that the chlamydial plasmid-encoded protein pGP3 forms a stable complex with LL-37 to neutralize its pro-inflammatory activity. Here, we explored whether pGP3 can inhibit the development of lesions in mice with imiquimod-induced psoriasis. MATERIAL AND METHODS The protein pGP3 was expressed in bacteria and purified using glutathione-conjugated agarose beads and a precision protease. The ability of the purified pGP3 to block chemotaxis mediated by LL-37 was tested in vitro using bone marrow-derived neutrophils. The ability of the protein to inhibit the development of psoriasis-like lesions was tested by topically or subcutaneously administering pGP3 in doses of 10 or 50 µg to mice previously treated with imiquimod. Mouse skin was evaluated using the psoriasis area and severity index (PASI) score and photography. Skin biopsies were taken on day 8 and analyzed histologically. RESULTS Purified pGP3 inhibited LL-37-mediated chemotaxis. Mice treated with 50 µg pGP3 showed clinical improvement with less severe erythema, infiltration, and scales; these mice also showed thinner dermis and less hyperkeratosis, parakeratosis, and inflammatory cell infiltration than mice treated with without 10 µg pGP3. CONCLUSIONS PGP3 can inhibit the development of psoriasis-like lesions in mice, possibly through its ability to bind LL-37. Future work should examine the mechanisms underlying this therapeutic effect.


Assuntos
Antígenos de Bactérias/farmacologia , Proteínas de Bactérias/farmacologia , Psoríase/patologia , Psoríase/terapia , Aminoquinolinas/farmacologia , Animais , Peptídeos Catiônicos Antimicrobianos/metabolismo , Peptídeos Catiônicos Antimicrobianos/farmacologia , Modelos Animais de Doenças , Feminino , Imiquimode , Camundongos , Camundongos Endogâmicos BALB C , Pele/patologia
18.
Neuroimage ; 169: 323-333, 2018 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-29221752

RESUMO

Deficits in the computational processes of reinforcement learning have been suggested to underlie addiction. Additionally, environmental uncertainty, which is encoded in the anterior cingulate cortex (ACC), modulates reward prediction errors (RPEs) during reinforcement learning and exacerbates addiction. The present study tested whether and how the ACC would have an essential role in drug addiction by failing to use uncertainty to modulate the RPEs during reinforcement learning. In Experiment I, we found that the ACC/medial prefrontal cortex (MPFC) did not modulate RPE learning according to uncertainty in smokers. The effect of uncertainty × RPE in the ACC/MPFC was correlated with the learning rate of RPEs and the duration of nicotine use. Experiment II demonstrated that serotonin, but not dopamine, receptor mRNA expression significantly decreased in the ACC of the nicotine exposed compared to the control rats. Furthermore, there was a positive correlation between learning rate and serotonin receptor mRNA expression in the ACC. Therefore, all present results suggest that impairments in uncertainty modulation in the ACC disrupt reinforcement learning processes in chronic nicotine users and contribute to maladaptive decision-making. These findings support interventions for pathological decision-making in drug addiction that strongly focus on the serotonin system in ACC.


Assuntos
Conectoma/métodos , Tomada de Decisões/fisiologia , Função Executiva/fisiologia , Giro do Cíngulo/fisiologia , Nicotina/efeitos adversos , Córtex Pré-Frontal/fisiologia , Receptores de Serotonina/efeitos dos fármacos , Incerteza , Animais , Comportamento Animal/efeitos dos fármacos , Tomada de Decisões/efeitos dos fármacos , Função Executiva/efeitos dos fármacos , Giro do Cíngulo/diagnóstico por imagem , Giro do Cíngulo/efeitos dos fármacos , Giro do Cíngulo/metabolismo , Humanos , Imagem por Ressonância Magnética , Masculino , Nicotina/administração & dosagem , Córtex Pré-Frontal/diagnóstico por imagem , Córtex Pré-Frontal/efeitos dos fármacos , Córtex Pré-Frontal/metabolismo , Ratos , Ratos Sprague-Dawley , Recompensa , Fumantes
19.
Cell Tissue Bank ; 19(1): 47-59, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28808811

RESUMO

Articular cartilage injury is a common type of damage observed in clinical practice. A matrix-induced autologous chondrocyte implant was developed to repair articular cartilage as an advancement on the autologous chondrocyte implant procedure. Here, we establish a thin double layer of collagen as a novel and effective bioscaffold for the regeneration of cartilaginous lesions. We created a collagen membrane with double layers using a cover slip, a cover slip, and the collagen was then freeze-dried under vacuum. Carbodiimide as a crosslinking agent was used to obtain a relatively stable collagen construction. The thickness of the knee joint cartilage from grown rabbits was measured from a frozen section. Both type I and type II collagens were characterized using Sodium dodecylsulfate/polyacrylamide gel electrophoresis (SDS-PAGE) and ultraviolet absorption peaks. The aperture size of the scaffold was observed using a scanning electron microscope (SEM). The degradation of the scaffolds in vitro was tested through digestion using collagenase solution. The mechanical capacity of the scaffolds was assessed under dynamic compression. The influence of the scaffold on chondrocyte proliferation was assessed using the methyl thiazolyl tetrazolium (MTT) colourimetric assay and scanning electron microscopy. The frozen sections of the rabbit femoral condyle showed that the thickness of the weight-bearing area of the articular cartilage was less than 1 mm. The results of the SDS-PAGE and ultraviolet absorption peaks of the collagens were in agreement with the standard photographs in the references. SEM showed that the aperture size of the cross-linked scaffold was 82.14 ± 15.70 µm. The in vitro degradation studies indicated that Carbodiimide cross-linking can effectively enhance the biostability of the scaffolds. The Carbodiimide cross-linking protocol resulted in a mean value for the samples that ranged from 8.72 to 15.95 MPa for the compressive strength. The results of the MTT demonstrated that the scaffold had promoted chondrocyte proliferation and SEM observations showed that the scaffold was a good adhesive and growth material for chondrocytes. Thin type I/II collagen composite scaffold can meet the demands of cartilage tissue engineering and have good biocompatibility.


Assuntos
Condrócitos/citologia , Colágeno Tipo II/química , Colágeno Tipo I/química , Tecidos Suporte/química , Animais , Materiais Biocompatíveis/química , Proliferação de Células , Células Cultivadas , Força Compressiva , Articulação do Joelho/ultraestrutura , Teste de Materiais , Coelhos , Engenharia Tecidual/métodos
20.
Int J Ophthalmol ; 10(8): 1280-1289, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28861356

RESUMO

AIM: To evaluate the efficacy and safety of anti-vascular endothelial growth factor (VEGF) combined with photodynamic therapy (PDT) versus anti-VEGF monotherapy for polypoidal choroidal vasculopathy (PCV). METHODS: We conducted a Meta-analysis of 9 studies to compare the efficacy and safety between combined therapy and anti-VEGF monotherapy for PCV. The programs of RevMan 5.3 and Stata 12.0 were used to analyze data. RESULTS: The best corrected visual acuity (BCVA) in combined therapy group were significantly better than those of anti-VEGF monotherapy group at 6, 24 and 36mo, with pooled weighted means differences (WMDs) of 0.12 (0.06, 0.18), 0.25 (0.12, 0.38) and 0.28 (0.13, 0.43), respectively. The central retinal thickness (CRT) reductions in combined therapy group were higher than that in anti-VEGF monotherapy group at 1, 3, 6 and 9mo, with pooled WMDs of 63.90 (20.41, 107.38), 33.47 (4.69, 62.24), 30.57 (0.12, 60.01) and 28.00 (2.51, 53.49), respectively. The regression rate of polyps in combined therapy group was much higher than that in anti-VEGF monotherapy group [RD: 0.47 (0.26, 0.68); P<0.0001]. The adverse event retinal hemorrhage did not differ significantly between the two groups. CONCLUSION: Our findings clearly document that anti-VEGF combined with PDT is a more effective therapy for PCV compared with anti-VEGF monotherapy. Furthermore, combined therapy does not increase the incidence of retinal hemorrhage.

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