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1.
Talanta ; 203: 227-234, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31202330

RESUMO

In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO2 nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO2/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue product in the presence of H2O2. Catalytic kinetic of IrO2/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO2/rGO nanocomposites originated from their ability of electron transfer between substrate and H2O2. On the basis of high peroxidase-like activity of IrO2/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO2/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment.

2.
Nanoscale ; 11(18): 9185-9193, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31038146

RESUMO

Recent advances in nanotechnology have developed a lot of opportunities for biological applications. In this work, multifunctional MoS2/AuNR nanocomposites with unique high NIR absorption were designed via combining MoS2 nanosheets and gold nanorods (AuNRs). The nanocomposites were synthesized through electrostatic self-assembly and showed high stability and good biocompatibility. Then they were used to modulate the aggregation of amyloid-ß peptides, destabilize mature fibrils under NIR irradiation, and eliminate Aß-induced ROS against neurotoxicity. The inhibition and destabilization effects were confirmed by Thioflavin T (ThT) fluorescence assay and transmission electron microscopy (TEM). Cell viability assay and ROS assay revealed that MoS2/AuNR nanocomposites could alleviate Aß-induced oxidative stress and cell toxicity. More importantly, both MoS2 nanosheets and AuNRs can be used as NIR photothermal agents, MoS2/AuNR nanocomposites have enhanced ability of disrupting Aß fibrils and improved cell viability by generating local heat under low power NIR irradiation. Our results provide new insights into the design of new multifunctional systems for the treatment of amyloid-related diseases.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Raios Infravermelhos , Nanocompostos/química , Fragmentos de Peptídeos/metabolismo , Amiloide/química , Amiloide/toxicidade , Peptídeos beta-Amiloides/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Dissulfetos/química , Ouro/química , Humanos , Molibdênio/química , Nanocompostos/toxicidade , Nanotubos/química , Fragmentos de Peptídeos/química , Espécies Reativas de Oxigênio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier
3.
J Colloid Interface Sci ; 539: 575-584, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30611053

RESUMO

Development of effective inhibitors toward Aß aggregation and reactive oxygen species (ROS) scavengers are of crucial therapeutic implications for Alzheimer's disease (AD). Herein, a novel agent with dual enzyme mimic activities has been fabricated as a multifunctional Aß fibrillation modulator. MoO3-x nanodots were synthesized by pulsed laser ablation (PLA) method in MoS2 nanosheets solutions, which may act directly as numerous fine targets. MoO3-x nanodots showed a uniform and monodispersed morphology, and the tiny dots were around 3-5 nm with a narrow size distribution. Due to the efficient charge transition between Mo5+/Mo6+ on the dots surface, MoO3-x nanodots exhibited excellent catalase and SOD mimic activities, which were adopted to alleviate Aß-mediated oxidative stress. Moreover, MoO3-x nanodots can efficiently inhibit Aß aggregation and destabilize the preformed fibrils, and eventually protect neuronal cells from apoptosis induced by Aß. Taken together, MoO3-x nanodots with multifunctional roles can act as a potential therapeutic strategy for treatment of amyloid induced neurotoxicity.


Assuntos
Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/toxicidade , Molibdênio/química , Molibdênio/farmacologia , Nanopartículas/química , Neurônios/efeitos dos fármacos , Neurônios/patologia , Óxidos/química , Óxidos/farmacologia , Apoptose/efeitos dos fármacos , Catalase/química , Sobrevivência Celular/efeitos dos fármacos , Humanos , Molibdênio/metabolismo , Neurônios/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Óxidos/metabolismo , Tamanho da Partícula , Agregados Proteicos/efeitos dos fármacos , Espécies Reativas de Oxigênio/análise , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/química , Propriedades de Superfície , Células Tumorais Cultivadas
4.
Colloids Surf B Biointerfaces ; 162: 296-305, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29216517

RESUMO

Nanomaterials with visible light-driven photocatalytic activity have attracted much attention due to their excellent abilities in degradation of various organic pollutants as well as inactivating bacteria. Herein, graphene oxide (GO) enwrapped silver chloride/silver (AgCl/Ag) nanocomposites with high visible light absorption were designed and fabricated as efficient antibacterial agents. AgCl NPs were synthesized in the presence of GO first and Ag NPs were coated on AgCl surface by heat reduction to form GO-AgCl/Ag nanocomposites. The as prepared nanocomposites revealed improved stability, higher absorption properties in the visible light region. The enhanced antibacterial activity was observed by quantification of colony forming units (CFU) and morphological changes of bacteria. The antibacterial mechanism of GO-AgCl/Ag was also investigated by evaluating membrane permeability and ROS level. Moreover, GO-AgCl/Ag composites can eliminate bacterial biofilms more efficiently under visible light irradiation. Our results provide new insights into the design of new multifunctional systems for antibacterial applications.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Grafite/farmacologia , Nanopartículas Metálicas/química , Nanocompostos/química , Compostos de Prata/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Animais , Antibacterianos/química , Biofilmes/efeitos da radiação , Catálise , Sobrevivência Celular/efeitos dos fármacos , Grafite/química , Luz , Nanopartículas Metálicas/efeitos da radiação , Nanopartículas Metálicas/ultraestrutura , Camundongos , Células NIH 3T3 , Nanocompostos/efeitos da radiação , Nanocompostos/ultraestrutura , Óxidos , Processos Fotoquímicos , Compostos de Prata/química , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus aureus/efeitos da radiação
5.
Colloids Surf B Biointerfaces ; 159: 540-545, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-28846964

RESUMO

Inhibiting amyloid ß (Aß) aggregation has drawn much attention because it is one of the main reasons for the cause of Alzheimer's disease (AD). Here we have synthesized a nanocomposite of graphene oxide-iron oxide (GOIO) and demonstrated its ability of modulating Aß aggregation. The inhibition effects of the GOIO nanocomposite on Aß aggregates was studied by Thioflavin T fluorescence assay, circular dichroism and transmission electron microscopy, respectively. Furthermore, the cell viability study revealed that the GOIO nanocomposite can reduce the toxicity of Aß fibrils to neuroblastoma cells. Our results demonstrated that the combination of GO and IO as a nanocomposite material has a potential use for the design new therapeutic agents for the treatment of Alzheimer's disease.


Assuntos
Peptídeos beta-Amiloides/química , Compostos Férricos/química , Grafite/química , Nanocompostos/química , Óxidos/química , Fragmentos de Peptídeos/química , Amiloide/química , Linhagem Celular Tumoral , Dicroísmo Circular , Humanos , Microscopia Eletrônica de Transmissão , Tiazóis/química
6.
ACS Appl Mater Interfaces ; 9(25): 21116-21123, 2017 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-28613069

RESUMO

The complex pathogenic mechanisms of Alzheimer's disease (AD) include the aggregation of ß-amyloid peptides (Aß) into oligomers or fibrils as well as Aß-mediated oxidative stress, which require comprehensive treatment. Therefore, the inhibition of Aß aggregation and free-radical scavenging are essential for the treatment of AD. Nanoparticles (NPs) have been found to influence Aß aggregation process in vitro. Herein, we report the inhibition effects of molybdenum disulfide (MoS2) NPs on Aß aggregation. Polyvinylpyrrolidone-functionalized MoS2 NPs were fabricated by a pulsed laser ablation method. We find that MoS2 NPs exhibit multifunctional effects on Aß peptides: inhibiting Aß aggregation, destabilizing Aß fibrils, alleviating Aß-induced oxidative stress, as well as Aß-mediated cell toxicity. Moreover, we show that MoS2 NPs can block the formation of the Ca2+ channel induced by Aß fibrils in the cell membrane for the first time. Thus, these observations suggest that MoS2 NPs have great potential for a multifunctional therapeutic agent against amyloid-related diseases.

7.
Analyst ; 142(13): 2500-2506, 2017 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-28589198

RESUMO

Due to low cost and high stability, the applications of inorganic nanomaterials as efficient alternatives to natural enzymes are drawing much attention. In this work, novel CuO/Pt nanocomposites with high peroxidase-like activity were designed and applied for the colorimetric detection of ascorbic acid (AA). The nanocomposites were prepared by decorating Pt NPs on the surface of CuO nanosheets, which displayed good uniformity and showed improved distribution and stability. The catalytic activity of the prepared CuO/Pt nanocomposites was tested against various chromogenic substrates in the presence of H2O2, which displayed efficient peroxidase-like activity and high catalytic stability against temperature. The catalytic mechanism of the CuO/Pt nanocomposites was investigated by hydroxyl radical detection. The peroxidase-like activity decreased significantly in the presence of AA. On the basis of the inhibition property, a colorimetric biosensor was constructed by using the CuO/Pt nanocomposites for the detection of AA. It showed a high selectivity against amino acids, carbohydrates and normal ions. Thus, this work provides new insights into the application of inorganic nanocomposite-based nanozymes in the biosensing field.

8.
Nanoscale ; 9(18): 5927-5934, 2017 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-28436514

RESUMO

Single or few-layered MoS2 nanosheets, as a novel class of 2D nanomaterials, have received tremendous attention due to their fantastic physical and chemical properties. Here, we fabricated MoS2-PEG-CpG with a small and uniform size as a multifunctional platform for photothermal enhanced immunotherapy. MoS2 nanosheets were fabricated by chemical exfoliation and further probe sonication. To realize MoS2-based adjuvant delivery, MoS2 nanosheets were functionalized with cytosine-phosphate-guanine (CpG) and polyethylene glycol (PEG) to form MoS2-PEG-CpG nanoconjugates. As an efficient nanocarrier with excellent near infrared-light (NIR) absorbing performance, MoS2-PEG-CpG significantly promotes CpG intracellular accumulation and the effect can be further enhanced by photothermal treatment. In addition, the enhanced uptake can stimulate the production of proinflammatory cytokines and remarkably elevate the immune response level. Finally, we found that MoS2-PEG-CpG could reduce the proliferative activity of cancer cells when co-cultured with a macrophage-like cell upon NIR irradiation, implying a novel strategy for multifunctional therapeutics against cancers.


Assuntos
Ilhas de CpG , Imunoterapia , Molibdênio , Nanoconjugados , Neoplasias/tratamento farmacológico , Fototerapia , Animais , Linhagem Celular Tumoral , Camundongos , Camundongos Endogâmicos C57BL , Células RAW 264.7
9.
ACS Nano ; 10(4): 4143-53, 2016 04 26.
Artigo em Inglês | MEDLINE | ID: mdl-26982522

RESUMO

Inhibition of amyloid aggregation is important for developing potential therapeutic strategies of amyloid-related diseases. Herein, we report that the inhibition effect of a pristine peptide motif (KLVFF) can be significantly improved by introducing a terminal regulatory moiety (terpyridine). The molecular-level observations by using scanning tunneling microscopy reveal stoichiometry-dependent polymorphism of the coassembly structures, which originates from the terminal interactions of peptide with organic modulator moieties and can be attributed to the secondary structures of peptides and conformations of the organic molecules. Furthermore, the polymorphism of the peptide-organic coassemblies is shown to be correlated to distinctively different inhibition effects on amyloid-ß 42 (Aß42) aggregations and cytotoxicity.


Assuntos
Peptídeos beta-Amiloides/química , Oligopeptídeos/química , Fragmentos de Peptídeos/química , Piridinas/química , Peptídeos beta-Amiloides/toxicidade , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Microscopia de Força Atômica , Microscopia de Tunelamento , Estrutura Molecular , Fragmentos de Peptídeos/toxicidade , Agregados Proteicos , Estrutura Secundária de Proteína , Piridinas/toxicidade , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier
10.
Nanoscale ; 8(6): 3685-93, 2016 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-26811962

RESUMO

To extend the functionalities of two-dimensional graphene-like layered compounds as versatile materials, the modification of transition metal dichalcogenide nanosheets such as MoS2 with metal nanoparticles is of great and widespread interest. However, few studies are available on the preparation of bimetallic nanoparticles supported on MoS2. Herein, a facile and efficient method to synthesize MoS2-PtAg nanohybrids by decorating ultrathin MoS2 nanosheets with octahedral Pt74Ag26 alloy nanoparticles has been reported. The as-prepared MoS2-Pt74Ag26 nanohybrids were investigated as novel peroxidase mimics to catalyze the oxidation of classical peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2, producing a blue colored reaction and exhibiting typical Michaelis-Menten kinetics. MoS2-Pt74Ag26 has a higher affinity for H2O2 than horseradish peroxidase (HRP) and a higher vmax value with TMB as the substrate than MoS2. The improved catalytic activity of hybrids for colorimetric reactions could be attributed to the synergistic effects of octahedral Pt74Ag26 nanoparticles and ultrathin MoS2 nanosheets as supports. Meanwhile, the generation of active oxygen species (˙OH) by H2O2 decomposition with MoS2-Pt74Ag26 was responsible for the oxidation of TMB. On the basis of these findings, a colorimetric method based on MoS2-Pt74Ag26 nanohybrids that is highly sensitive and selective was developed for glucose detection. Lower values of the limit of detection (LOD) were obtained, which is more sensitive than MoS2 nanosheets.


Assuntos
Materiais Biomiméticos/química , Dissulfetos/química , Glucose/análise , Peróxido de Hidrogênio/análise , Membranas Artificiais , Nanopartículas Metálicas/química , Molibdênio/química , Peroxidase/química , Platina/química , Prata/química
11.
ACS Appl Mater Interfaces ; 8(4): 2511-6, 2016 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-26745637

RESUMO

Nanowires have attracted much attention due to their potential bioapplications, such as delivery of drugs or sensing devices. Here we report the development of a unique cell-capture and release platform based on nanowires. The combination of nanowires, surface-binding peptides, and cell-targeting aptamers leads to specific and efficient capture of cancer cells. Moreover, the binding processes are reversible, which is not only useful for downstream analysis but also for reusability of the substrate. Our work provides a new method in the design of the cell-capture and release platform, which may open up new opportunities of developing cell-separation and diagnosis systems based on cell-capture techniques.


Assuntos
Aptâmeros de Peptídeos/química , Nanofios/química , Adsorção , Gálio/química , Humanos , Células MCF-7 , Microscopia de Fluorescência
12.
Phys Chem Chem Phys ; 17(33): 21576-82, 2015 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-26220437

RESUMO

Today cancer is one of the most life-threatening diseases in the world. The conventional cancer therapies, including surgery, chemo- and radiation therapies, have some disadvantages, such as limited efficiency and significant side effects. It is necessary to develop new therapeutic treatments. Herein, we integrated the targeted photocatalytic and chemotherapy in a multifunctional drug-delivery platform. The aptamer-functionalized ZnO nanoparticles (NPs) were successfully synthesized. The anti-cancer drug was loaded in the aptamer-ZnO NP system. In vitro cell cytotoxicity experiments showed that combined therapy had a higher rate of death of cancer cells compared to that of single photocatalytic or chemotherapy. Furthermore, aptamer-functionalization could greatly increase the accumulation of nanoparticles within cancer cells and lead to better therapeutic effects. The results suggest that aptamer-functionalized semiconductor nanoparticles may have potential in the development of targeted photocatalytic and chemotherapy against cancer.


Assuntos
Antineoplásicos/química , Aptâmeros de Nucleotídeos/química , Portadores de Fármacos/química , Nanopartículas Metálicas/química , Óxido de Zinco/química , Antineoplásicos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Doxorrubicina/toxicidade , Células HEK293 , Humanos , Luz , Células MCF-7 , Silanos/química
13.
ACS Nano ; 8(9): 9503-10, 2014 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-25192556

RESUMO

Amyloid peptides are considered to be the main contributor for the membrane disruption related to the pathogenesis of degenerative diseases. The variation of amino acids at the carboxylic terminus of amyloid peptide has revealed significant effects on the modulation of abnormal assemblies of amyloid peptides. In this work, molecular binding agents were tethered to the C-terminus of ß-amyloid peptide 1-42 (Aß42). The molecular interaction between Aß42 and molecule tethers was identified at single molecule level by using scanning tunneling microscopy (STM). The mechanistic insight into the feature variation of the self-assembly of Aß42 peptide caused by molecular tethering at C-terminus was clearly revealed, which could appreciably affect the nucleation of amyloid peptide, thus reducing the membrane disruptions.


Assuntos
Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/metabolismo , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Peptídeos beta-Amiloides/toxicidade , Linhagem Celular Tumoral , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Humanos , Cinética , Modelos Moleculares , Fragmentos de Peptídeos/toxicidade , Agregados Proteicos/efeitos dos fármacos , Ligação Proteica , Multimerização Proteica , Estrutura Secundária de Proteína , Piridinas/metabolismo , Piridinas/farmacologia
14.
Colloids Surf B Biointerfaces ; 123: 293-301, 2014 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-25264283

RESUMO

Semiconductors are important materials used for the development of high-performance biomedical devices. Gallium nitride (GaN) is a well-known III-nitride semiconductor with excellent optoelectronic properties as well as high chemical stability and biocompatibility. The formation of tight interfaces between GaN substrates and cells would be crucial for GaN-based devices used for probing and manipulating biological processes of cells. Here we report a strategy to greatly enhance cell adhesion and survival on nanotextured GaN surface which was treated by UV illumination and fibronectin (FN) adsorption. Cell studies showed that the UV/FN treatment greatly enhanced cell adhesion and growth on nanotextured GaN surfaces. These observations suggest new opportunities for novel nanotextured GaN-based biomedical devices.


Assuntos
Fibronectinas/química , Gálio/química , Gálio/farmacologia , Animais , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Humanos , Camundongos , Células NIH 3T3 , Nanoestruturas/química , Semicondutores , Raios Ultravioleta
15.
Small ; 10(21): 4386-94, 2014 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-25059878

RESUMO

A novel and convenient method to synthesize the nanocomposites combining graphene oxides (GO) with gold nanoparticles (AuNPs) is reported and their applications to modulate amyloid peptide aggregation are demonstrated. The nanocomposites produced by pulsed laser ablation (PLA) in water show good biocompatibility and solubility. The reduced aggregation of amyloid peptides by the nanocomposites is confirmed by Thioflavin T fluorescence and atomic force microscopy. The cell viability experiments reveals that the presence of the nanocomposites can significantly reduce the cytotoxicity of the amyloid peptides. Furthermore, the depolymerization of peptide fibrils and inhibition of their cellular cytotoxicity by GO/AuNPs is also observed. These observations suggest that the nanocomposites combining GO and AuNPs have a great potential for designing new therapeutic agents and are promising for future treatment of amyloid-related diseases.


Assuntos
Proteínas Amiloidogênicas/metabolismo , Ouro/química , Grafite/química , Terapia a Laser/métodos , Lasers , Nanocompostos/química , Agregação Patológica de Proteínas/prevenção & controle , Água/química , Proteínas Amiloidogênicas/química , Sobrevivência Celular , Células Cultivadas , Humanos , Terapia a Laser/instrumentação , Teste de Materiais , Óxidos/química , Peptídeos/química , Peptídeos/metabolismo , Agregação Patológica de Proteínas/metabolismo
16.
ACS Appl Mater Interfaces ; 5(19): 9816-22, 2013 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-24073887

RESUMO

Interfacing nanowires with living cells is attracting more and more interest due to the potential applications, such as cell culture engineering and drug delivery. We report on the feasibility of using photoresponsive semiconductor gallium nitride (GaN) nanowires (NWs) for regulating the behaviors of biomolecules and cells at the nano/biointerface. The GaN NWs have been fabricated by a facile chemical vapor deposition method. The superhydrophobicity to superhydrophilicity transition of the NWs is achieved by UV illumination. Bovine serum albumin adsorption could be modulated by photoresponsive GaN NWs. Tunable cell detachment and adhesion are also observed. The mechanism of the NW surface responsible for modulating both of protein adsorption and cell adhesion is discussed. These observations of the modulation effects on protein adsorption and cell adhesion by GaN NWs could provide a novel approach toward the regulation of the behaviors of biomolecules and cells at the nano/biointerface, which may be of considerable importance in the development of high-performance semiconductor nanowire-based biomedical devices for cell culture engineering, bioseparation, and diagnostics.


Assuntos
Gálio/química , Nanotecnologia , Nanofios/química , Soroalbumina Bovina/química , Adsorção/efeitos dos fármacos , Animais , Bovinos , Adesão Celular/efeitos dos fármacos , Gálio/farmacologia , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Células NIH 3T3 , Semicondutores , Propriedades de Superfície
17.
Nanoscale ; 5(22): 11019-27, 2013 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-24068181

RESUMO

We report a general approach for the synthesis of large-scale gallium nitride (GaN) nanostructures by the graphene oxide (GO) assisted chemical vapor deposition (CVD) method. A modulation effect of GaN nanostructures on cell adhesion has been observed. The morphology of the GaN surface can be controlled by GO concentrations. This approach, which is based on the predictable choice of the ratio of GO to catalysts, can be readily extended to the synthesis of other materials with controllable nanostructures. Cell studies show that GaN nanostructures reduced cell adhesion significantly compared to GaN flat surfaces. The cell-repelling property is related to the nanostructure and surface wettability. These observations of the modulation effect on cell behaviors suggest new opportunities for novel GaN nanomaterial-based biomedical devices. We believe that potential applications will emerge in the biomedical and biotechnological fields.


Assuntos
Gálio/química , Grafite/química , Nanoestruturas/química , Animais , Plaquetas/citologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Humanos , Células MCF-7 , Camundongos , Microscopia Confocal , Células NIH 3T3 , Nanoestruturas/toxicidade , Óxidos/química , Propriedades de Superfície
18.
Nanoscale ; 4(6): 2078-82, 2012 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-22331105

RESUMO

We report a study of nanoribbons of quercetin, a phase I clinical trial anticancer drug, and their inhibitory effects on cancer cell proliferation. Novel quercetin nanoribbons have been prepared by atmospheric pressure physical vapor deposition (PVD). The nanostructures have been characterized by optical microscopy, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy, etc. Significantly enhanced solubility in PBS solution and increased drug release rate have been observed for quercetin nanoribbons in comparison to those of quercetin powder. The observed increase of inhibitory effects of quercetin nanoribbons on 4T1 cancel cell growth is correlated with an improvement in their solubility and drug release behavior.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Sobrevivência Celular/efeitos dos fármacos , Composição de Medicamentos/métodos , Nanopartículas/química , Nanopartículas/uso terapêutico , Quercetina/síntese química , Quercetina/uso terapêutico , Animais , Antioxidantes/síntese química , Antioxidantes/uso terapêutico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sinergismo Farmacológico , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Nanopartículas/ultraestrutura
19.
ACS Nano ; 5(2): 1276-81, 2011 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-21230008

RESUMO

We report on the synthesis of inorganic fullerene-like molybdenum disulfide (MoS(2)) nanoparticles by pulsed laser ablation (PLA) in water. The final products were characterized by scanning electron microscopy, X-ray diffraction, transmission electron microscopy, and resonance Raman spectroscopy, etc. Cell viability studies show that the as-prepared MoS(2) nanoparticles have good solubility and biocompatibility, which may show a great potential in various biomedical applications. It is shown that the technique of PLA in water also provides a green and convenient method to synthesize novel nanomaterials, especially for biocompatible nanomaterials.


Assuntos
Materiais Biocompatíveis/química , Dissulfetos/química , Fulerenos/química , Lasers , Molibdênio/química , Nanopartículas/química , Água/química , Materiais Biocompatíveis/toxicidade , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Humanos , Modelos Moleculares , Conformação Molecular , Nanopartículas/toxicidade , Solubilidade
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