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1.
IUCrJ ; 9(Pt 3): 355-363, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35546804

RESUMO

The effects of synthesis time on the plasmonic properties of Ag dendritic nanoforests on Si substrate (Ag-DNF/Si) samples synthesized through the fluoride-assisted galvanic replacement reaction were investigated. The Ag-DNF/Si samples were characterized using scanning electron microscopy, energy-dispersive X-ray spectroscopy, reflection spectroscopy, X-ray diffraction and surface-enhanced Raman spectroscopy (SERS). The prolonged reaction time led to the growth of an Ag-DNF layer and etched Si hole array. SEM images and variations in the fractal dimension index indicated that complex-structure, feather-like leaves became coral-like branches between 30 and 60 min of synthesis. The morphological variation during the growth of the Ag DNFs resulted in different optical responses to light illumination, especially those of light harvest and energy transformation. The sample achieved the most desirable light-to-heat conversion efficiency and SERS response with a 30 min growth time. A longer synthesis time or thicker Ag-DNF layer on the Si substrate did not have superior plasmonic properties.

2.
Front Pharmacol ; 13: 912153, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35571073

RESUMO

Background: Evidence of osimertinib as neoadjuvant therapy for resectable non-small cell lung cancer (NSCLC) are currently lacking. This case series study aimed to assess the safety and feasibility of neoadjuvant osimertinib therapy followed by surgery for resectable NSCLC. Materials and methods: Patients with resectable NSCLC with epidermal growth factor receptor (EGFR) mutation who received osimertinib as neoadjuvant therapy followed by surgery at our center were included. Demographic features, radiologic and pathological assessment of response, surgery-related details and complications, toxicity profiles, and prognostic outcomes were extracted. Results: A total of 13 patients were included in this study. The median age at the time of surgical resection was 57 years (interquartile range: 52-64 years), and eight (61.5%) patients were female. The objective response rate (ORR) was 69.2% (9/13), and the complete resection rate was 100%. The rates of pathologic downstaging and lymph node downstaging were 100% (13/13) and 66.7% (6/9), respectively. There were no perioperative deaths and only three (23.1%) patients had postoperative complications. Seven (53.8%) and 13 (100%) patients experienced grade 1 treatment-related adverse reactions and laboratory abnormalities, respectively. No patients experienced drug withdrawal or surgical delays due to the adverse events. No patients showed grade 2 or worse toxicity profiles. One patient was lost to follow-up. The other 12 patients were alive and free of disease recurrence with a median follow-up time of 9.5 months. Conclusion: Neoadjuvant osimertinib therapy seemed to be safe and feasible for resectable EGFR-mutated NSCLC. Future large prospective studies are warranted to confirm whether osimertinib as neoadjuvant therapy outperforms standard tyrosine kinase inhibitors (TKIs) or chemotherapy for resectable EGFR-mutated NSCLC.

3.
Environ Res ; 212(Pt D): 113465, 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35594959

RESUMO

Mass production of microalgal biodiesel is hindered by microalgae harvesting efficiency and costs. In this study, Daphnia domesticated by amino acids were used to harvest microalgae via ingesting. The main factors (density of Daphnia, salinity, pH, light-environment, temperature and algal concentration) that were conducive to Daphnia feeding were optimized. Under the optimal condition, Microalgae-feeding Daphnia were domesticated by adding D-glutamic acid and L-cysteine as stimulating factors. After that, the ingestion rate of domesticated Daphnia increased by 24.93%. The presence of Daphnia as a predator can induce microalgae to mass into clusters. Combining Daphnia feeding and the inductive defense flocculation of microalgae, the harvesting rate of mixed algae (Chlorella pyrenoidosa and Scenedesmus obliquus) reached over 95% after 9 h. Overall, this work suggested that Daphnia feeding process is a green and economical approach for microalgae harvesting.

5.
Artigo em Inglês | MEDLINE | ID: mdl-35606568

RESUMO

The advent of drug-eluting stents and drug-coated balloons have significantly improved the clinical outcome of patients with vascular occlusions. However, ischemic vascular disease remains the most common cause of death worldwide. Improving the current treatment modalities demands a better understanding of the processes which govern drug uptake and retention in blood vessels. In this study, we evaluated the influence of urea and butyryl-trihexyl citrate, as excipients, on the efficacy of drug-coated balloon therapy. An integrated approach, utilizing both in-vitro and in-silico methods, was used to quantify the tracking loss, vessel adhesion, drug release, uptake, and distribution associated with the treatment. Moreover, a parametric study was used to evaluate the potential influence of different types of lesions on drug-coated balloon therapy. Despite the significantly higher tracking loss (urea: 35.5% vs. butyryl-trihexyl citrate: 8.13%) observed in the urea-based balloons, the drug uptake was almost two times greater than with its hydrophobic counterpart. Non-calcified lesions were found to delay the transmural propagation of sirolimus while calcification was shown to limit the retentive potential of lesions. Ultimately this study helps to elucidate how different excipients and types of lesions may influence the efficacy of drug-coated balloon therapy.

6.
Chemistry ; 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35608191

RESUMO

AlCl3-NaCl was utilized as an electrolyte in this work due to its low melting point and Lewis acidity, in which samarium exists in two oxidation states, Sm(III) and Sm(II), resulting in unique electrochemical behaviours. Sm metal dissolves in AlCl3-NaCl melt to form SmCl2, which is verified by electrochemical and spectroscopic techniques. As the Lewis acidity of the melt increases, the diffusion coefficient of Sm(II) gradually increases, and the activation energy of diffusion decreases. Moreover, an additional co-reduction peak of Sm3+ and AlCl4- is observed to be more positive than that of Al(0)/Al(III) in Lewis basic melt, which may be tightly correlated with the variation of Sm(II) coordination in AlCl3-NaCl melt and ligand variation from Cl- to AlCl4- and Al2Cl7- as the Lewis acidity of the AlCl3-NaCl melt increases, according to the in-situ electronic absorption spectra of Sm in this melt.

8.
HLA ; 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35524576

RESUMO

HLA-C*01:212 differs from HLA-C*01:02:01:01 by two non-synonmous nucleotide changes at positions 368 and 379 in exon 3.

9.
HLA ; 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35524579

RESUMO

HLA-B*13:157 has one nucleotide change from HLA-B*13:02:01:01 at nucleotide 323 changing Tyrosine to Phenylalanine at residue 84.

10.
HLA ; 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35524580

RESUMO

HLA-C*15:244 has one nucleotide change from HLA-C*15:05:01:01 at nucleotide 308 changing Arginine to Glutamine at residue 79.

11.
HLA ; 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35524588

RESUMO

HLA-B*35:251:02 has one nucleotide change from HLA-B*35:22:01:01 at nucleotide 363 changing Serine to Arginine at residue 97.

12.
Anal Chim Acta ; 1208: 339843, 2022 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-35525593

RESUMO

The chemistry of the metal-organic frameworks (MOFs) coating may affect the biological functionality of the encapsulated biomacromolecules in harsh environment. Enzymes encapsulated in hydrophilic MAF-7 can retain high activity in harsh environment. We conducted this study to prepare a non-invasive wearable uircase@MAF-7-based electrochemical sensor that can achieve accurate and sensitive detection of UA levels in sweat by integrating a flexible microfluidic chip and wireless electronic readout device. The flexible microfluidic chip enabled an easy and effective collection of sweat samples. MAF-7 protected enzyme activity by encapsulating uricase. The uricase@MAF-7-based electrochemical sensor enabled the highly sensitive detection of UA in the concentration range of 2 µM-70 µM with a detection limit of as low as 0.34 µM. Additionally, we evaluated the utility of the sensor for monitoring UA levels in real sweat samples by means of a high purine dietary challenge. This personalized wearable sweat sensing device has a potential to be used for monitoring disease-related metabolites in daily life.


Assuntos
Técnicas Biossensoriais , Estruturas Metalorgânicas , Dispositivos Eletrônicos Vestíveis , Suor/química , Urato Oxidase , Ácido Úrico/análise
13.
Ann Transl Med ; 10(7): 420, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35530953

RESUMO

Background: The hospitalization for asthma exacerbation has varied with seasons, however, the underlying weather reasons have not been fully explored yet. This study is aimed to explore the effect of weather factors on increased number of hospitalization due to worsening of asthma symptoms. This will provide more information to the relevant authorities to allocate appropriate medical resources as per the weather conditions in Qingdao, China. Methods: All adult patients admitted for asthma exacerbation from 1 January, 2017 to 31 December, 2019 were enrolled from 13 main hospitals of Qingdao. The clinical data, including age, sex, smoking history, etc., were collected from the electronic medical record (EMR) systems. The hourly air quality of Qingdao from 2017-2019, including the air quality index (AQI), PM2.5 and PM10, was obtained from the China National Environmental Monitoring Centre. All these parameters during 2017-2019 were compared monthly. For meteorological data, the monthly horizontal wind at 850 hPa and vertical velocity at 500 hPa during 1960-2020 were obtained from National Center for Environmental Prediction (NCEP) and the National Center for Atmospheric Research (NCAR) global reanalysis dataset. The correlation analysis was applied to determine the association between asthma hospitalizations and the environmental factors, including atmospheric pressure, humidity, vertical visibility, and etc., monthly. Results: In all, 10,549 asthmatic inpatients (45.7% males, 54.3% females) were included in the study. The inpatients number for asthma exacerbation had a plateau lasting from March to June of 2019, accompanied with high PM2.5 and PM10, as well as bad air quality from January to March of 2019, potentially governed by the El Niño event in 2018. However, there was no significance correlation between the number of asthma hospitalizations and the average value of all environmental factors. Conclusions: The high rate of hospitalization for asthma exacerbation in Qingdao during the spring of 2019 was associated with the unfavorable weather conditions, which might be linked to the atmospheric circulation in East Asia.

14.
Neural Regen Res ; 17(11): 2399-2403, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35535877

RESUMO

Spinal cord injury (SCI), either from trauma or degenerative changes, can result in severe disability and impaired quality of life. Understanding the cellular processes and molecular mechanisms that underlie SCI is imperative to identifying molecular targets for potential therapy. Recent studies have shown that non-coding RNAs, including both long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), regulate various cellular processes in SCI. In this review, we will describe the changes in lncRNA and circRNA expression that occur after SCI and how these changes may be related to SCI progression. Current evidence for the roles of lncRNAs and circRNAs in neuronal cell death and glial cell activation will also be reviewed. Finally, the possibility that lncRNAs and circRNAs are novel modulators of SCI pathogenesis will be discussed.

15.
Artigo em Chinês | MEDLINE | ID: mdl-35511621

RESUMO

Objective:To investigate the difference between manual reduction and automatic device reduction in the treatment of benign paroxysmal positional vertigo(BPPV), and to provide evidence-based medicine for the clinical choice of BPPV treatment. Methods:Two hundred and two BPPV patients who came to the hospital for diagnosis and treatment were collected and divided into two groups by random number table method. Group A had 102 cases for manual reduction, and group B had 100 cases for automatic device reduction. Both groups were given the same medicine-assisted treatment. All patients were followed up 7 to 10 days after reduction treatment. To evaluate the differences in the overall effective rate of treatment, visual analog scale(VAS), incidence of adverse reactions, treatment time were compared between the two groups. Results:The overall effective rate was 98.03% and 91.00% in group A and group B, respectively, group A was slightly higher than group B(P=0.027); the difference in VAS scores before and after treatment: group A was 6(4) points, group B was 5(3) , group A is greater than group B(P=0.002); adverse reaction rates in groups A and B were 4.90% and 8.00%, respectively, group B was slightly higher than group A(P=0.37); treatment time: group A 6.0(1.0) min in group A and 8.0(2.0) min in group B, group A was significantly shorter than group B(P<0.01). Conclusion:Both manual and fully automatic device reduction can effectively improve the clinical symptoms of BPPV patients, but for physicians with extensive clinical experience it is recommended to choose manual reduction.


Assuntos
Vertigem Posicional Paroxística Benigna , Procedimentos Cirúrgicos Reconstrutivos , Vertigem Posicional Paroxística Benigna/diagnóstico , Medicina Baseada em Evidências , Humanos , Medição da Dor/efeitos adversos , Estudos Prospectivos
16.
Int J Ophthalmol ; 15(5): 683-689, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35601157

RESUMO

AIM: To investigate the mechanism of the tight junction (TJ) disruption and the association between tumor necrosis factor (TNF)-α and matrix metalloproteinase (MMPs) under hyperosmotic condition in primary human corneal epithelial cells (HCECs). METHODS: The cultured HCECs were exposed to media which adding sodium chloride (NaCl) for hyperosmolar stress or adding rh-TNF-α (10 ng/mL). NF-κB inhibitor (5 µmol/L) or GM-6001 (potent and broad spectrum MMP inhibitor, 20 µmol/L) was added 1h before that treatment. The integrity of TJ proteins was determined by immunofluorescent (IF) staining. The mRNA levels of TNF-α and MMPs were evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and the protein expression by enzyme-linked immunosorbent assay (ELISA). RESULTS: TJ proteins ZO-1 and Occludin were disrupted in primary HCECs exposed to hyperosmotic medium. The mRNA expression and protein production of TNF-α increased significantly in hyperosmotic media at 500 mOsM. TNF-α mediated the expression and production of MMP-1, MMP-13, MMP-9, and MMP-3 stimulated by hyperosmotic stress. The production of MMPs in hyperosmolar media were increased through the increase of TNF-α. GM-6001 prevent the destruction of ZO-1 and Occludin in hyperosmolar stress and rh-TNF-α treated medium. TNF-α induced activation of MMPs was involved in the TJ disruption by hyperosmolarity. CONCLUSION: TJ proteins ZO-1 and Occludin are disrupted by hyperosmolar stress and TNF-α, but protected by MMP inhibitor (GM-6001). It suggests that TNF-α/MMP pathway mediates the TJ disruption in primary HCECs exposed to hyperosmotic stress.

17.
J Chem Inf Model ; 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35561002

RESUMO

Atomic descriptions of peptide self-assembly are crucial to an understanding of disease-related peptide aggregation and the design of peptide-assembled materials. Obtaining these descriptions through computer simulation is challenging because current force fields, which were not designed for this process and are often unable to describe correctly peptide self-assembly behavior and the sequence dependence. Here, we developed a framework using dipeptide aggregation as a model system to improve force fields for simulations of self-assembly. Aggregation-related structural properties were designed and used to guide the optimization of peptide-peptide and peptide-solvent interactions. With this framework, we developed a self-assembly force field, termed PACE-ASM, by reoptimizing a hybrid-resolution force field that was originally developed for folding simulation. With its applicability in folding simulations, the new PACE was used to simulate the self-assembly of two disease-related short peptides, Aß16-21 and PHF6, into ß-sheet-rich cross-ß amyloids. These simulations reproduced the crystal structures of Aß16-21 and PHF6 amyloids at near-atomic resolution and captured the difference in packing orientations between the two sequences, a task which is challenging even with all-atom force fields. Apart from cross-ß amyloids, the self-assembly of emerging helix-rich cross-α amyloids by another peptide PSMα3 can also be correctly described with the new PACE, manifesting the versatility of the force field. We demonstrated that the ability of the PACE-ASM to model peptide self-assembly is based largely on its improved description of peptide-peptide and peptide-solvent interactions. This was achieved with our optimization framework that can readily identify and address the deficiency in describing these interactions.

18.
Exp Hematol Oncol ; 11(1): 25, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505384

RESUMO

BACKGROUND: Acute graft-versus-host disease (aGVHD) remains the major cause of early mortality after haploidentical related donor (HID) hematopoietic stem cell transplantation (HSCT). We aimed to establish a comprehensive model which could predict severe aGVHD after HID HSCT. METHODS: Consecutive 470 acute leukemia patients receiving HID HSCT according to the protocol registered at https://clinicaltrials.gov (NCT03756675) were enrolled, 70% of them (n = 335) were randomly selected as training cohort and the remains 30% (n = 135) were used as validation cohort. RESULTS: The equation was as follows: Probability (grade III-IV aGVHD) = [Formula: see text], where Y = -0.0288 × (age) + 0.7965 × (gender) + 0.8371 × (CD3 + /CD14 + cells ratio in graft) + 0.5829 × (donor/recipient relation) - 0.0089 × (CD8 + cell counts in graft) - 2.9046. The threshold of probability was 0.057392 which helped separate patients into high- and low-risk groups. The 100-day cumulative incidence of grade III-IV aGVHD in the low- and high-risk groups was 4.1% (95% CI 1.9-6.3%) versus 12.8% (95% CI 7.4-18.2%) (P = 0.001), 3.2% (95% CI 1.2-5.1%) versus 10.6% (95% CI 4.7-16.5%) (P = 0.006), and 6.1% (95% CI 1.3-10.9%) versus 19.4% (95% CI 6.3-32.5%) (P = 0.017), respectively, in total, training, and validation cohort. The rates of grade III-IV skin and gut aGVHD in high-risk group were both significantly higher than those of low-risk group. This model could also predict grade II-IV and grade I-IV aGVHD. CONCLUSIONS: We established a model which could predict the development of severe aGVHD in HID HSCT recipients.

19.
Light Sci Appl ; 11(1): 126, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35513383

RESUMO

Signal conversion plays an important role in many applications such as communication, sensing, and imaging. Realizing signal conversion between optical and microwave frequencies is a crucial step to construct hybrid communication systems that combine both optical and microwave wireless technologies to achieve better features, which are highly desirable in the future wireless communications. However, such a signal conversion process typically requires a complicated relay to perform multiple operations, which will consume additional hardware/time/energy resources. Here, we report a light-to-microwave transmitter based on the time-varying and programmable metasurface integrated with a high-speed photoelectric detection circuit into a hybrid. Such a transmitter can convert a light intensity signal to two microwave binary frequency shift keying signals by using the dispersion characteristics of the metasurface to implement the frequency division multiplexing. To illustrate the metasurface-based transmitter, a hybrid wireless communication system that allows dual-channel data transmissions in a light-to-microwave link is demonstrated, and the experimental results show that two different videos can be transmitted and received simultaneously and independently. Our metasurface-enabled signal conversion solution may enrich the functionalities of metasurfaces, and could also stimulate new information-oriented applications.

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