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1.
Natl Sci Rev ; 8(7): nwaa236, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34691688

RESUMO

The minimized diffusion limitation and completely exposed strong acid sites of the ultrathin zeolites make it an industrially important catalyst especially for converting bulky molecules. However, the structure-controlled and large-scale synthesis of the material is still a challenge. In this work, the direct synthesis of the single-layer MWW zeolite was demonstrated by using hexamethyleneimine and amphiphilic organosilane as structure-directing agents. Characterization results confirmed the formation of the single-layer MWW zeolite with high crystallinity and excellent thermal/hydrothermal stability. The formation mechanism was rigorously revealed as the balanced rates between the nucleation/growth of the MWW nanocrystals and the incorporation of the organosilane into the MWW unit cell, which is further supported by the formation of MWW nanosheets with tunable thickness via simply changing synthesis conditions. The commercially available reagents, well-controlled structure and the high catalytic stability for the alkylation of benzene with 1-dodecene make it an industrially important catalyst.

2.
Mar Pollut Bull ; 173(Pt B): 113086, 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34695688

RESUMO

In this study, the sorption behaviors and mechanisms between polystyrene microplastics (micro-PS) and 4-rings polycyclic aromatic hydrocarbons (PAHs) pyrene (Pyr) and its derivatives (S-Pyr), including 1-methylpyrene (P-CH3), 1-hydroxypyrene (P-OH), 1-aminopyrene (P-NH2), 1-pyrenecarboxylic acid (P-COOH) were investigated at neutrality. The results revealed that the sorption rates of micro-PS for S-Pyr were higher than those for parent Pyr. Meanwhile, -CH3 could slightly facilitate the sorption, whereas -OH, P-NH2, and P-COOH intensively inhibit the sorption of S-Pyr onto micro-PS. The sorption capacities of Pyr/S-Pyr increased with decreasing size of micro-PS. Besides, the effects of salinity and temperature on the sorption characteristics of micro-PS for Pyr/S-Pyr depended on their substituents. Combined with experimental and computational methods, it could be concluded that the main sorption mechanisms were possibly hydrophobic interaction, π-π interaction and pore-filling. The observations reported here could improve predictions of environmental behaviors and bioavailability of PAHs and micro-PS.

3.
Ann Palliat Med ; 10(8): 8818-8826, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34488370

RESUMO

BACKGROUND: Roughly 30-40% of lung cancer (LC) patients develop bone metastasis during the course of disease. The genetic differences between primary LC and matched bone metastasis are not yet fully understood. METHODS: A total of 40 LC patients with bone metastasis were collected and 450 targeted cancer-related genes were sequenced for genomic-alteration (GA) identification. RESULTS: Among the 40 LC patients, 33 had adenocarcinomas and 7 had squamous cell carcinomas. The metastatic sites of the 33 lung adenocarcinomas (LUADs) were the pelvis (6 patients), spine (16 patients), and limbs (11 patients). A total of 425 and 422 GAs were detected in the primary and metastatic lesions, respectively. The most common GAs were epidermal growth factor receptor (EGFR) mutations, which had mutation rates of 85.0% and 72.5% in the primary and metastatic lesions, respectively, and tumor protein 53 (TP53) mutations, which had mutation rates of 52.5% and 67.5% in the primary and metastatic lesions, respectively. Metastases to the pelvis and spine were most commonly accompanied by factor receptor substrate 2 (FRS2), cyclin-dependent kinase 4 (CDK4), and murine double minute 2 (MDM2) amplification, and cyclin-dependent kinase inhibitor 2A (CDKN2A) deletion. The concordance between primary lung squamous cell carcinoma (LUSC) and corresponding metastasis was significantly higher than that of primary LUAD and corresponding metastasis (P=0.033). Compared to limb and pelvis metastases, the shared mutation in spine metastasis was significantly lower (P=0.016 and P=0.023, respectively). In matched primary LUSCs and bone metastasis lesions, there was no significant difference in the distribution of the tumor mutational burden (TMB) (P=0.9). Conversely, a significant difference of the TMB distribution was detected in pairs of primary LUAD and corresponding bone metastasis lesions (P=0.021). CONCLUSIONS: The consistency of mutation patterns between primary LC lesions and matched bone metastases may vary in terms of metastatic sites, but is very high in general. There was a significant difference in the TMB between primary LUAD and matched bone metastatic lesions. Our findings contribute to molecular understandings of primary LC and matched bone metastatic lesions.


Assuntos
Neoplasias Ósseas , Neoplasias Pulmonares , Adenocarcinoma/genética , Adenocarcinoma/patologia , Neoplasias Ósseas/genética , Neoplasias Ósseas/secundário , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Mutação , Metástase Neoplásica/genética
4.
Angew Chem Int Ed Engl ; 60(42): 22933-22939, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34431192

RESUMO

A built-in electric field in electrocatalyst can significantly accumulate higher concentration of NO3 - ions near electrocatalyst surface region, thus facilitating mass transfer for efficient nitrate removal at ultra-low concentration and electroreduction reaction (NO3 RR). A model electrocatalyst is created by stacking CuCl (111) and rutile TiO2 (110) layers together, in which a built-in electric field induced from the electron transfer from TiO2 to CuCl (CuCl_BEF) is successfully formed . This built-in electric field effectively triggers interfacial accumulation of NO3 - ions around the electrocatalyst. The electric field also raises the energy of key reaction intermediate *NO to lower the energy barrier of the rate determining step. A NH3 product selectivity of 98.6 %, a low NO2 - production of <0.6 %, and mass-specific ammonia production rate of 64.4 h-1 is achieved, which are all the best among studies reported at 100 mg L-1 of nitrate concentration to date.

5.
Sci Total Environ ; 793: 148664, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34328991

RESUMO

The influence of long-term fertilization on soil microbial communities is critical for revealing the association between belowground microbial flora and aboveground crop productivity-a relationship of great importance to food security, environmental protection, and ecosystem functions. Here, we examined shifts in soil chemical properties, microbial communities, and the nutrient uptake and yield of rice subjected to different chemical and organic fertilization treatments over a 40-year period in red paddy soil. Ten different treatments were used: a control without fertilizer, and applications of nitrogen (N), phosphorus (P), potassium (K), NP, NK, PK, NPK, double NPK, or NPK plus manure. Compared with the effects of withholding one or two nutrients (N, P, or K), the balanced application of chemical NPK and organic fertilizers markedly improved soil nutrient status and rice yield. This improvement of soil fertility and rice yield was not associated with bacterial, archaeal, or fungal alpha diversities. The bacterial abundance and community structure and archaeal abundance effectively explained the variation in rice yield, whereas those of fungi did not. The community structure of bacteria and archaea, but not that of fungi, was correlated with soil properties. Among various soil properties, P was the key factor influencing rice yield and soil microbial communities because of the extremely low content of soil available P. Seven keystones at the operational taxonomic unit level were identified: four archaea (belonging to Thermoplasmata, Methanosaeta, Bathyarchaeia, and Nitrososphaeraceae) and three bacteria (in Desulfobacteraceae and Acidobacteriales). These keystones, which were mainly related to soil C and N transformation and pH, may work cooperatively to influence rice yield by regulating soil fertility. Our results collectively suggest that four decades of balanced fertilization has sustained the bacterial and archaeal abundances, bacterial community structure, and keystones, which potentially contribute to soil fertility and rice yield in red paddy soil.


Assuntos
Microbiota , Oryza , Agricultura , Fertilização , Fertilizantes/análise , Esterco , Solo , Microbiologia do Solo
6.
Chem Commun (Camb) ; 57(62): 7669-7672, 2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34254065

RESUMO

A novel nanozyme comprised of graphene encapsuled Ru nanocrystals (Ru@G) with effective and stable peroxidase-like activity prepared using a chemical vapor deposition (CVD) method was used for the detection of glutathione at near-physiological pH.


Assuntos
Materiais Biomiméticos/química , Glutationa/análise , Grafite/química , Nanopartículas Metálicas/química , Peroxidase/metabolismo , Rutênio/química , Concentração de Íons de Hidrogênio
7.
Huan Jing Ke Xue ; 42(7): 3482-3493, 2021 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-34212675

RESUMO

As the problem of global warming becomes increasingly serious, the greenhouse gas (GHG) emission reduction measures of constructed wetlands (CWs) have drawn significant attention. Ferric-carbon micro-electrolysis exhibits an excellent effect on wastewater purification as well as the potential to reduce GHG emissions. Therefore, to explore the impact of ferric-carbon micro-electrolysis on GHG emissions from intermittent aeration constructed wetlands, four kinds of different wetlands with different fillers were constructed. The four fillers were ferric-carbon micro-electrolysis filler+gravel (CW-Ⅰ), ferric-carbon micro-electrolysis filler+zeolite (CW-Ⅱ), zeolite (CW-Ⅲ), and gravel (CW-Ⅳ). Intermittent aeration technology was used to aerate the wetland systems. The results show that ferric-carbon micro-electrolysis significantly improved the nitrogen removal efficiency of the intermittent aeration constructed wetlands and reduced GHG emissions. Compared with CW-Ⅳ, the CH4 fluxes of CW-Ⅰ, CW-Ⅱ, and CW-Ⅲ decreased by 32.81% (P<0.05), 52.66% (P<0.05), and 54.50% (P<0.05), respectively. Among them, zeolite exhibited a stronger reduction effect on CH4 emissions in both the aeration and non-aeration sections. The ferric-carbon micro-electrolysis substantially reduced N2O emissions. In comparison with CW-Ⅳ, CW-, and CW-Ⅱ achieved N2O emission reduction by 30.29%-60.63% (P<0.05) and 43.10%-73.87% (P<0.05), respectively. During a typical hydraulic retention period, the comprehensive GWP caused by CH4 and N2O emitted by each group of wetland system are (85.21±6.48), (49.24±3.52), (127.97±11.44), and (137.13±11.45) g·m-2, respectively. The combined use of ferric-carbon micro-electrolysis and zeolite effectively reduces GHG emissions in constructed wetlands. Overall, ferric-carbon micro-electrolysis combined with zeolite (CW-Ⅱ) can be regarded as one of the valuable filler combination methods for constructed wetlands, which can ensure high removal efficiency of pollutants and effective GHG emission reduction in constructed wetlands.


Assuntos
Gases de Efeito Estufa , Carbono , Dióxido de Carbono/análise , Eletrólise , Efeito Estufa , Gases de Efeito Estufa/análise , Metano/análise , Óxido Nitroso/análise , Áreas Alagadas
8.
Front Immunol ; 12: 682103, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34113354

RESUMO

Accurate prediction of neoantigens and the subsequent elicited protective anti-tumor response are particularly important for the development of cancer vaccine and adoptive T-cell therapy. However, current algorithms for predicting neoantigens are limited by in vitro binding affinity data and algorithmic constraints, inevitably resulting in high false positives. In this study, we proposed a deep convolutional neural network named APPM (antigen presentation prediction model) to predict antigen presentation in the context of human leukocyte antigen (HLA) class I alleles. APPM is trained on large mass spectrometry (MS) HLA-peptides datasets and evaluated with an independent MS benchmark. Results show that APPM outperforms the methods recommended by the immune epitope database (IEDB) in terms of positive predictive value (PPV) (0.40 vs. 0.22), which will further increase after combining these two approaches (PPV = 0.51). We further applied our model to the prediction of neoantigens from consensus driver mutations and identified 16,000 putative neoantigens with hallmarks of 'drivers'.

9.
Cardiovasc Diabetol ; 20(1): 126, 2021 06 24.
Artigo em Inglês | MEDLINE | ID: mdl-34167539

RESUMO

BACKGROUND: It remains unclear whether triglyceride-glucose (TyG) index, a surrogate marker of insulin resistance, is prospectively associated with incident peripheral arterial disease (PAD). METHODS: We included 12,320 Atherosclerosis Risk in Communities Study participants (aged 54.3 ± 5.7 years) free of a history of PAD at baseline (visit 1: 1987-1989). The TyG index was determined using ln (fasting triglycerides [mg/dL] × fasting glucose [mg/dL]/2), and measured at 5 visits between 1987 and 2013. Incident PAD was defined as the first hospitalization with PAD diagnosis or a new onset of measured ABI < 0.90 during follow-up visits. We quantified the association of both baseline and trajectories of TyG index with incident PAD using Cox regression and logistic regression analysis, respectively. RESULTS: Over a median follow-up of 23 years, 1300 participants developed PAD. After adjustment for traditional PAD risk factors, each 1-SD (0.58) increase in TyG index was associated with an 11.9% higher risk of incident PAD [hazard ratio, 1.119 (95% CI, 1.049-1.195)]. Results were similar when individuals were categorized by TyG index quartiles [hazard ratio, 1.239 (95% CI, 1.028-1.492); comparing extreme quartiles]. Four distinct trajectories of stable TyG indexes at various levels along the follow-up duration were identified [low (22.2%), moderate (43.2%), high (27.5%), and very high (7.1%) trajectory groups]. Compared with those with a TyG index trajectory at a low level, those participants with TyG index trajectories at high and very high levels had an even greater risk of future incident PAD [odds ratio (95%CI): 1.404 (1.132-1.740) and 1.742 (1.294-2.344), respectively] after multivariate adjustments for traditional PAD risk factors. CONCLUSIONS: Higher TyG index is independently associated with an increased risk of incident PAD. Long-term trajectories of TyG index help identify individuals at a higher risk of PAD who deserve specific preventive and therapeutic approaches. TRIAL REGISTRATION: Clinical trial registration number: The ARIC trial was registered at clinicaltrials.gov as NCT00005131.

10.
PLoS One ; 16(5): e0249663, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34043642

RESUMO

Long non-coding RNA (lncRNA) of plant species undergoes dynamic regulation and acts in developmental and stress regulation. Presently, there is little information regarding the identification of lncRNAs in jujube (Ziziphus jujuba Mill.), and it is uncertain whether the lncRNAs could respond to heat stress (HS) or not. In our previous study, a cultivar (Hqing1-HR) of Z. jujuba were treated by HS (45°C) for 0, 1, 3, 5 and 7 days, and it was found that HS globally changed the gene expression by RNA sequencing (RNA-seq) experiments and informatics analyses. In the current study, 8260 lncRNAs were identified successfully from the previous RNA-seq data, and it indicated that lncRNAs expression was also altered globally, suggesting that the lncRNAs might play vital roles in response to HS. Furthermore, bioinformatics analyses of potential target mRNAs of lncRNAs with cis-acting mechanism were performed, and it showed that multiple differentially expressed (DE) mRNAs co-located with DElncRNAs were highly enriched in pathways associated with response to stress and regulation of metabolic process. Taken together, these findings not only provide a comprehensive identification of lncRNAs but also useful clues for molecular mechanism response to HS in jujube.


Assuntos
RNA Longo não Codificante/metabolismo , Transcriptoma , Ziziphus/genética , Genoma de Planta , RNA de Plantas/química , RNA de Plantas/metabolismo , Análise de Sequência de RNA , Temperatura , Fatores de Tempo , Ziziphus/metabolismo
11.
Huan Jing Ke Xue ; 42(6): 2875-2884, 2021 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-34032087

RESUMO

Ferric-carbon micro-electrolysis fillers and zeolite have been increasingly used as substrates in constructed wetlands due to their good wastewater pollution-removal efficiencies. To explore the effects of different fillers on wastewater treatment in constructed wetlands, four constructed wetlands were examined with vertical subsurface flow areas filled with ferric-carbon micro-electrolysis filler+gravel (CW-A), ferric-carbon micro-electrolysis filler+zeolite (CW-B), zeolite (CW-C), and gravel (CW-D). In addition, intermittent aeration was used to improve the dissolved oxygen (DO) environment. The results showed that, compared with CW-D, the ferric-carbon micro-electrolysis filler significantly increased the dissolved oxygen (DO, P<0.05) and pH (P<0.05) of the effluent from the wetlands. The mean removal efficiency of chemical oxygen demand (COD) in the four constructed wetlands were more than 95% (P>0.05). For TN, the mean removal efficiency of CW-A,-B, and-C was 7.94% (P<0.05), 9.29% (P<0.05), and 3.63% (P<0.05) higher than that of CW-D, respectively. The contribution of ferric-carbon micro-electrolysis filler and zeolite to improving the TN removal efficiency of the constructed wetlands was 73.55% and 26.45%, respectively. The mean removal efficiency of NH4+ in the four wetlands ranged from 67.93% to 76.90%, and compared with CW-D, the other treatments significantly improved the removal efficiency of NH4+ (P<0.05). The ferric-carbon micro-electrolysis filler had an excellent removal effect on NO3-, with a removal efficiency of more than 99%, which was significantly higher than the constructed wetlands without ferric-carbon micro-electrolysis (P<0.05). Considering the treatment effect of the organic pollutants and the nitrogen-containing pollutants, CW-B achieved the best removal efficiency in constructed wetlands with intermittent aeration.

12.
Pharmazie ; 76(4): 165-171, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33849702

RESUMO

Our paper investigated the effect of berberine on the diabetic retinopathy (DR) in db/db transgenic mice and explored its possible mechanisms. During chronic intragastric administration for ten weeks, berberine could decrease the levels of fasting blood glucose, TC and TG without hepatotoxicity. Moreover, berberine could protect the retinal morphology against the hyperglycemic insults and decrease glycogen accumulation, the contents of TNF-α and IL-1ß in the retinas, as demonstrated by HE staining, PAS staining and ELISA kits, respectively. Immunofluorescence assay revealed that the protein expression of vascular endothelial growth factor (VEGF), VEGF receptor 2, hypoxia-inducible factor-1α(HIF-1α), and nuclear factor-κ B (NF-κB) p65 was upregulated in db/db retinas compared with wild type ones, whereas berberine treatment could suppress their expression. Berberine prevent DR development through modulating the glucolipid metabolism and inhibiting the HIF-1α /VEGF/NF-κ B pathway, suggesting that berberine maybe a potential agent for the treatment of DR.

13.
Aging (Albany NY) ; 13(9): 12456-12465, 2021 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-33891562

RESUMO

Inherited predispositions to acute lymphoblastic leukemia have been well investigated in pediatric patients, but studies on adults, particularly Chinese patients, are limited. In this study, we conducted a genome-wide association study in 466 all-age Chinese patients with Acute lymphoblastic leukemia (ALL) and 1,466 non-ALL controls to estimate the impact of age on ALL susceptibility in the Chinese population. Among the 17 reported loci, 8 have been validated in pediatric and 1 in adult patients. The strongest association signal was identified at ARID5B locus and gradually decreased with age, while the signal at GATA3 exhibited the opposite trend and significantly impact on adult patients. With genome-wide approaches, germline variants at 2q14.3 rank as the top inherited predisposition to adult patients (e.g., rs73956024, P = 4.3 × 10-5) and separate the genetic risk of pediatric vs. adult patients (P = 3.6 × 10-6), whereas variants at 15q25.3 (e.g., rs11638062) have a similar impact on patients in different age groups (overall P = 2.9 × 10-7). Our analysis highlights the impact of age on genetic susceptibility to ALL in Chinese patients.


Assuntos
Fatores Etários , Proteínas de Ligação a DNA/genética , Predisposição Genética para Doença/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Adulto , Grupo com Ancestrais do Continente Asiático , Criança , Pré-Escolar , Feminino , Fator de Transcrição GATA3/genética , Estudo de Associação Genômica Ampla , Humanos , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Fatores de Risco , Fatores de Transcrição/genética
14.
Huan Jing Ke Xue ; 42(3): 1559-1568, 2021 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742953

RESUMO

Investigate the effects of plastic film mulching on CH4 and N2O emissions from a vegetable field, a one-year in situ field observation was conducted using a static opaque chamber in a pepper-radish cropping system at the Key Field Station for Monitoring of Eco-Environment of Purple Soil of the Ministry of Agriculture of China at Southwest University, Chongqing. Two treatments (conventional and film mulching) were used to study the influence of film mulching on CH4 and N2O emissions. The results showed that mulching significantly increased the annual average soil pH (P<0.01), annual surface and subsurface (5 cm) temperature (P<0.05), and soil moisture content during the radish-growing season (P<0.05). The mulching also significantly reduced CH4 emissions in the field ridges (P<0.05); the average CH4 flux from ridges during the pepper-growing season was 0.110 mg·(m2·h)-1 and 0.028 mg·(m2·h)-1, and 0.011 mg·(m2·h)-1 and -0.019 mg·(m2·h)-1 during the radish-growing season, under the conventional and film mulching treatments, respectively. However, across the entire experiment, CH4 flux from field furrows was not significantly different between the two mulching treatments (P>0.05), with mean flux values during the pepper-growing season of 0.058 mg·(m2·h)-1 and 0.057 mg·(m2·h)-1, and 0.083 mg·(m2·h)-1 and 0.092 mg·(m2·h)-1 during the radish-growing season, for conventional and plastic film mulching, respectively. Except for the conventional treatment during the pepper-growing season, CH4 emissions from ridges were significantly higher than from furrows, but for other treatments, including conventional and film mulching treatments during radish-growing season and film mulching treatment during the pepper-growing season, the CH4 emissions from furrows were all significantly higher than those from ridges. This was related to the stable anoxic environment created in furrows under high rainfall conditions in Southwest China. The N2O emission flux from the ridges during the pepper-growing season was 65.41 µg·(m2·h)-1 and 68.39 µg·(m2·h)-1 under the conventional and film mulching treatments, respectively, and the N2O emission flux during the radish-growing season was 78.43 µg·(m2·h)-1 and 66.19 µg·(m2·h)-1, respectively. The N2O flux between conventional treatment and film mulching treatment in ridges or furrows were not significantly different (P>0.05), while the N2O emissions from the ridges were significantly higher than that from the furrows. CH4 emission flux was significantly positively correlated with surface and subsurface temperature, while N2O emission flux was only significantly positively correlated with alkaline nitrogen and ammonium nitrogen content.

15.
Angew Chem Int Ed Engl ; 60(22): 12498-12503, 2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-33756014

RESUMO

A Kagome structure covalent organic framework (COF) film with three-state NIR electrochromic properties was designed and synthesized. The COFTPDA-PDA film is composed of hexagonal nanosheets with high crystallinity and has three reversible color states at different applied potentials. It has high absorption spectra changes in the NIR region, ascribed to the strong intervalence charge transfer (IVCT) interaction of the Class III mixed-valence systems of the conjugated triphenylamine species. The film showed sub-second response time (1.3 s for coloring and 0.7 s for bleaching at 1050 nm) and long retention time in the NIR region. COFTPDA-PDA film shows superior NIR electrochromic properties in term of response time and stability, attributed to the highly ordered porous structure and the π-π stacking structure of the COFTPDA-PDA architecture. The COFTPDA-PDA film was applied in mimicking a flip-flop logic gate with optical memory function.

16.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 43(1): 32-36, 2021 Feb 28.
Artigo em Chinês | MEDLINE | ID: mdl-33663659

RESUMO

Objective To evaluate the effect of methylprednisolone sodium succinate combined with tropisetron on postoperative nausea and vomiting(PONV)under microvascular decompression of hemifacial spasm.Methods From January to June 2019,485 patients undergoing microvascular decompression for facial spasm at Department of Neurosurgery,Peking University People's Hospital were randomly assigned into two groups with random number table method.For group A(n=242),2 ml saline was administrated by intravenous drip before induction and 5 mg tropisetron after operation.For group B(n=243),40 mg methylprednisolone sodium succinate was administrated by intravenous drip before induction and 5 mg tropisetron after operation.The anesthesia time,operation time,and incidence of PONV in 0-24 h and 24-48 h were recorded for the comparison of the remedial treatment rate of nausea and vomiting between the two groups.Results There was no significant difference in age,gender,smoking history,body mass index value,American Society of Anesthesiologists score,medical history,surgical side,PONV history,operation time or anesthesia time between the two groups(all P > 0.05).The incidence of PONV in group A was 35.5% and 18.2% during 0-24 h and 24-48 h,respectively,which was significantly higher than that(18.5%,χ 2=7.331,P=0.007;8.2%,χ 2=4.364,P=0.037)in group B.The application rate of antiemetic drugs in group A was 15.2% and 8.7% during 0-24 h and 24-48 h,respectively,which was significantly higher than that(5.3%,χ 2=5.327,P=0.021;2.0%,χ 2=4.432,P=0.035)in group B.Conclusion The combination of methylprednisolone sodium succinate and tropisetron can effectively prevent PONV under microvascular decompression of hemifacial spasm,with the performance superior to single drug treatment.


Assuntos
Antieméticos , Espasmo Hemifacial , Cirurgia de Descompressão Microvascular , Método Duplo-Cego , Espasmo Hemifacial/tratamento farmacológico , Espasmo Hemifacial/cirurgia , Humanos , Indóis , Hemissuccinato de Metilprednisolona/uso terapêutico , Tropizetrona
17.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 43(1): 92-100, 2021 Feb 28.
Artigo em Chinês | MEDLINE | ID: mdl-33663669

RESUMO

Objective To analyze the research status,hotspots,and frontiers of atherosclerosis genomics from 2010 to 2019.Methods CiteSpace software was used to conduct data statistics and visual analysis on countries,institutions,authors,journals,co-cited papers,and keywords of the related papers published in the Web of Science from 2010 to 2019.Results A total of 1021 papers in English were included,and the annual number of publications generally showed an upward trend.The knowledge base in the research of atherosclerosis mostly focused on the genetic risk sites and biomarkers for coronary artery diseases such as coronary heart disease,myocardial infarction,and dyslipidemia.The related journals mainly involved the fields of molecular biology,biology,genetics,immunology,medicine,pharmacy,and clinical medicine.The latest research in atherosclerosis concentrated on genome-wide association study,DNA methylation,microRNA,messenger RNA and so on.The research frontiers involved long noncoding RNA,DNA methylation,and immune metabolism.Conclusion The studies in atherosclerotic genomics have gradually increased.


Assuntos
Aterosclerose , Bibliometria , Aterosclerose/genética , Biomarcadores , Estudo de Associação Genômica Ampla , Genômica , Humanos
18.
Front Microbiol ; 12: 626691, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33708183

RESUMO

Repeated stress-related diarrhea is a kind of functional bowel disorders (FBDs) that are mainly stemming from dysregulation of the microbiota-gut-brain axis mediated by a complex interplay of 5-hydroxytryptophan (5-HT). Intestinal content and intestinal mucosa microbiota belong to two different community systems, and the role of the two microbiota community systems in repeated stress-related diarrhea remains largely unknown. In order to ascertain the difference in composition and the potential function between intestinal content and intestinal mucosa microbiota response on repeated stress-related diarrhea, we collected intestinal contents and mucosa of mice with repeated stress-related diarrhea for 16S rRNA PacBio SMRT gene full-length sequencing, and with the digital modeling method of bacterial species abundance, the correlations among the two microbiota community systems and serum 5-HT concentration were analyzed. We found that the microbiotal composition differences both in intestinal contents and mucosa were consistent throughout all the phylogenetic ranks, with an increasing level of resolution. Compared with intestinal content microbiota, the diversity and composition of microbiota colonized in intestinal mucosa are more sensitive to repeated stress-related diarrhea. The PICRUSt2 of metagenomic function analysis found that repeated stress-related diarrhea is more likely to perturb the intestinal mucosa microbiota metagenomic functions involved in the neural response. We further found that the mucosal microbiota-based relative abundance model was more predictive on serum 5-HT concentration with the methods of machine-learning model established and multivariate dimensionality reduction (R 2 = 0.876). These findings suggest that the intestinal mucosa microbiota might serve as a novel potential prediction model for the serum 5-HT concentration involvement in the repeated stress-related diarrhea, in addition to focusing on its mechanism in the gastrointestinal dysfunction.

19.
Front Pharmacol ; 12: 626614, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33762946

RESUMO

Background: In postthyroidectomy patients, hypocalcemia is the most common complication to prolong hospital stay and decrease patients' satisfaction. Based on current evidence, it is recommended to supply vitamin D to patients with high risk of developing hypocalcemia. However, how to stratify the risk of patients remains challenging. Aim: We conducted a prospective study to evaluate the effect of vitamin D supplement (calcitriol) on high-risk hypocalcemia patients based on relative decline of parathyroid hormone (RDP). Method: RDP was calculated by the difference between preoperative and postoperative first-day PTH divided by preoperative PTH and presented as percentage. Patients who underwent total thyroidectomy in addition to bilateral central compartment dissection were enrolled prospectively and were divided into two cohorts: Cohort I: patients with RDP ≤70% and Cohort II: patients with RDP >70%. Patients in Cohort I were then randomly assigned to Group A or B, and patients in Cohort II were randomly assigned to Group C or D. All groups received oral calcium, and patients in Groups B and D also received calcitriol. All patients were followed for one year. In the study, standard procedure dictates that only oral calcium is given to patients whose RDP ≤70% and that oral calcium and calcitriol are given to patients whose RDP >70%. Therefore, Cohort I Group A and Cohort II Group D are controls in this study. Results: The incidence of clinical hypocalcemia in Groups A and D (the controls) was 11.0% (10/91), and 17.6% (16/91) required additional intravenous calcium. Of note, no patients developed permanent hypocalcemia. Furthermore, calcitriol supplement did not have significant impact on clinical outcomes between Group A and B in Cohort I. By contrast, calcitriol supplement distinctly improved clinical outcome by comparing Groups C and D (Cohort II), as marked by clinical hypocalcemia, need of requiring intravenous calcium, and long-termed decreased levels of PTH. Conclusion: Supplying calcitriol based on RDP cutoff of 70% may be a wise practice in thyroidectomy patients, and RDP 70% may be a useful predictor to stratify high-risk patients.

20.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(1): 188-192, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33554817

RESUMO

OBJECTIVE: To understand the carrying rate, gene mutation frequency and composition ratio of thalassemia in pregnant women in Suxian and Beihu districts of Chenzhou, Hunan Province. METHODS: Thalassemia gene in 11 212 samples was analyzed by using Next-Generation Sequencing. RESULTS: Among the 11 212 samples, 938 were diagnosed as thalassemia, in which 618 (5.51%) were diagnosed as α-thalassemia, 268 (2.39%) as ß-thalassemia, 29(0.26%)as abnormal hemoglobin and 23 (0.21%) as αß-thalassemia. The gene mutations of --SEA /αα(40.29%) and -α3.7/αα(37.7%) in α-thalassemia were the most common, while for ß- thalassemia, the most commonly gene mutation were ß41-42M/ßN(24.26%) and ß654M/ßN(23.88%). The detection rate of rare type α,ß-thalassemia gene was 0.19%(21/11 212), 0.53%(59/11 212), respectively. CONCLUSION: The carrying rate of thalassemia in pregnant women is 8.37% in Suxian and Beihu districts of Chenzhou city, and the genotypes are complex. Next-Generation Sequencing can detect rare thalassemia genes and new gene mutations effectively.


Assuntos
Hemoglobinas Anormais , Talassemia alfa , Talassemia beta , China , Feminino , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Mutação , Gravidez , Gestantes , Talassemia alfa/genética , Talassemia beta/genética
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