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1.
Eur Rev Med Pharmacol Sci ; 24(8): 4055, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32374004

RESUMO

The article "Long noncoding RNA NORAD promotes the progression of retinoblastoma by sponging miR-136-5p/PBX3 axis, by X.-L. Yang, Y.-J. Hao, B. Wang, X.-L. Gu, X.-X. Wang, J.-F. Sun, published in Eur Rev Med Pharmacol Sci 2020; 24(3):1278-1287. DOI: 10.26355/eurrev_202001_20185. PMID: 32096159" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause.

2.
Eur Rev Med Pharmacol Sci ; 24(3): 1278-1287, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32096159

RESUMO

OBJECTIVE: The specific roles of long noncoding RNAs (lncRNAs) have been found in human cancers, including retinoblastoma (RB). However, the function of lncRNA-NORAD has not been reported in RB. Therefore, the regulatory mechanism of lncRNA-NORAD was investigated in the development of RB. PATIENTS AND METHODS: The experimental tissues were collected from 24 RB patients and 6 patients with ruptured globes. The average age of all patients was 2.78 years (range, 2 months to 11 years). The mRNA and protein expression was measured by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) and Western blot analysis. The functional mechanism of NORAD was assessed by Cell Counting Kit-8 (CCK-8), transwell, and Dual-Luciferase reporter assays. RESULTS: Upregulation of NORAD and downregulation of miR-136-5p were found in RB. Functionally, knockdown of NORAD and miR-136-5p overexpression restrained RB cell viability, invasion, and migration. In addition, NORAD acts as a ceRNA of miR-136-5p in RB. MiR-136-5p was found to directly target PBX3. Furthermore, knockdown of PBX3 inhibited the progression of RB. More importantly, the NORAD/miR-136-5p axis is involved in RB progression by mediating PBX3. CONCLUSIONS: LncRNA NORAD, serving as a ceRNA of miR-136-5p, accelerates RB progression by upregulation of PBX3.

3.
Beijing Da Xue Xue Bao Yi Xue Ban ; 50(6): 1117-1119, 2018 Dec 18.
Artigo em Chinês | MEDLINE | ID: mdl-30562793

RESUMO

A 52-year-old man was referred to our department with a 2-year history of polyarthritis. He was diagnosed as gout due to acute arthritis of bilateral feet dorsum 2 years ago,but he didn't receive any standard treatment. 1 year ago,there were more and more joints evolved during the gout attack, and many subcutaneous nodules occurred. When he presented to our clinic 1 month ago,the urate acid level was as high as 715 µmol/L. Moreover, we could find bone erosion in the X rays of his hand and foot,as well as synovitis,double contour sign and tophus on the ultrasound examination. The diagnosis of gout was clearly and definitely. However, he had leukocytosis and thrombocytosis for 4 years in the past history, and the urate acid level was only 400 µmol/L at that time. He also had well-controlled hypertension. The family history was unremarkable. Furthermore, we found megalosplenia on his physical examination. The bone marrow examination showed myelofibrosis and JAK2 V617F gene was positive. He was diagnosed as primary myelofibrosis and treated with interferon-α, together with urate acid-lowing therapy (febuxostat 60 mg once daily). Following-up for 1 year,the dosage of febuxostat decreased to 40 mg once daily, and the patient didn't have gout attack again, some of the tophus diminished, and the urate acid level ranged from 400 to 500 µmol/L. Gout is a common disease in clinical practice,usually combined with metabolic syndrome,chronic renal failure and specific drugs using (diuretic and calcineurin inhibitors). However,it is relatively rare to see gout associated with myeloproliferative diseases, including polycythemia vera, primary thrombocythemia, primary myelofibrosis and chronic myelocytic leukemia. In these diseases, the turnover of nucleic acids is greatly augmented, and an excess of purine metabolites, including uric acid, is released. In the natural course of gout, the appearance of tophus from the first onset of arthritis usually takes several years. This patient only had one traditional risk factor, but his urate acid level was remarkably high and he developed tophus in a short term. After treatment of primary myelofibrosis, the symptom of gout partially alleviated. Careful physical examination and medical history taking lead to the diagnosis of secondary gout, which should be reminded in the daily practice.


Assuntos
Gota , Mielofibrose Primária , Artrite Gotosa/etiologia , Febuxostat/uso terapêutico , Gota/tratamento farmacológico , Gota/etiologia , Supressores da Gota/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade , Mielofibrose Primária/complicações , Ácido Úrico
4.
Eur Rev Med Pharmacol Sci ; 21(16): 3533-3537, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28925493

RESUMO

OBJECTIVE: This study was designed to investigate the clinical effects of adopting perforator pedicled propeller flap in the proper digital artery for treatment and sensory reconstruction of finger pulp defects in children. PATIENTS AND METHODS: Perforator pedicled propeller flap of proper digital arteries in thirty-one fingers from twenty-three children patients were selected for repairing the pulp defects on the 2nd to the 5th fingers. All cases were treated from September 2012 to December 2013. To properly reconstruct the pulps' feeling we needed the dorsal branch of the proper digital nerve in the flap to be consistent with the broken end of proper digital nerve in pulps' wound. A free skin graft was carried out with full-thickness skin taken from the medial thigh for flap donor area. We scheduled two post-operation return visits, one in six months and the second visit in twelve months following the operation. Parents' satisfaction with the postoperative appearance of their children's fingers was assessed based on Michigan Hand Outcome Questionnaire. RESULTS: All operations were performed successfully and all wounds healed in the first period and all flaps survived. During the final follow-up, the shape recovery of flaps and their donor areas were examined and satisfactory results were obtained. All pulps were full and round without any obvious pigmentation or scar contracture. The sensory recovery of pulps was achieved S3+, and the two-point discrimination was 4.5 to 6.0 mm (with 5.1 mm being the average value). Parents' satisfaction with the postoperative results was very promising with twenty-one cases of very satisfied and two cases of satisfied parents. CONCLUSIONS: The operation method used in this study demonstrated to be a safe and reliable procedure producing a very good flap blood supply as well as excellent sensory recovery and satisfactory postoperative appearance.


Assuntos
Traumatismos dos Dedos/cirurgia , Retalho Perfurante/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Transplante de Pele/métodos , Artérias , Criança , Pré-Escolar , Cicatriz/cirurgia , Feminino , Dedos/irrigação sanguínea , Mãos/cirurgia , Humanos , Masculino , Período Pós-Operatório , Pele , Cicatrização
5.
Dis Aquat Organ ; 125(1): 63-71, 2017 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-28627493

RESUMO

Parasitic disease is among the major causes of health problems in marine mammals. However, little information on parasitic species composition and infection levels in finless porpoises (Neophocaena spp.) is available. In this study, we report the first systematic survey on parasitic infections in the East Asian finless porpoise N. asiaeorientalis sunameri (EAFP) living off the Chinese Yellow/Bohai Sea coast. Using both morphological and molecular methods, 5 parasitic helminths were identified: Campula oblonga in the liver and pancreas; Synthesium seymouri in the pyloric stomach and duodenum ampulla; Anisakis pegreffii in the forestomach, main stomach, and esophagus; Halocercus sp. in the lungs; and Crassicauda magna in the mammary glands and muscle. Among these helminths, C. oblonga (80.7%), S. seymouri (80.7%), A. pegreffii (80.7%), and Halocercus sp. (77.4%) were the most prevalent, whereas C. magna (6.5%) were only observed in 2 EAFP individuals. All juvenile and adult EAFPs were parasitized by at least 3 parasites species (C. oblonga, S. seymouri, and A. pegreffii), whereas in neonates, only Halocercus sp. were detected. We observed no significant difference in parasite prevalence between males and females. In addition, A. pegreffii and C. magna represented new infection records in Neophocaena. A pathological examination associated with parasitic lesions in EAFPs showed damage or destruction of cells or tissues to some extent. This study represents the first systematic survey on parasitic infections in EAFPs, providing important and valuable parasitological information for the research and conservation of this coastal marine mammal.


Assuntos
Infecções por Nematoides/veterinária , Toninhas/parasitologia , Infecções por Trematódeos/veterinária , Animais , Feminino , Masculino , Nematoides/classificação , Nematoides/isolamento & purificação , Infecções por Nematoides/epidemiologia , Infecções por Nematoides/parasitologia , Oceano Pacífico/epidemiologia , Trematódeos/classificação , Trematódeos/isolamento & purificação , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/parasitologia
6.
Sci Rep ; 7: 46351, 2017 04 11.
Artigo em Inglês | MEDLINE | ID: mdl-28398291

RESUMO

The fine dissection of nerves and blood vessels in the tarsal tunnel is necessary for clinical operations to provide anatomical information. A total of 60 feet from 30 cadavers were dissected. Two imaginary reference lines that passed through the tip of the medial malleolus were applied. A detailed description of the branch pattern and the corresponding position of the posterior tibial nerve, posterior tibial artery, medial calcaneal nerve and medial calcaneal artery was provided, and the measured data were analyzed. Our results can be summarized as follows. I. A total of 81.67% of the bifurcation points of the posterior tibial nerve, which was divided into the medial and lateral plantar nerves, were located within the tarsal tunnel, not distal to the tarsal tunnel. II. The bifurcation points of the posterior tibial artery were all located in the tarsal tunnel. Almost all of the bifurcation points of the posterior tibial artery were lower than those of the posterior tibial nerve. The bifurcation point of the posterior tibial artery situated distal to the tarsal tunnel was not found. III. The number and the origin of the medial calcaneal nerves and arteries were highly variable.


Assuntos
Dissecação , Síndrome do Túnel do Tarso/cirurgia , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndrome do Túnel do Tarso/patologia , Síndrome do Túnel do Tarso/fisiopatologia , Artérias da Tíbia/patologia , Artérias da Tíbia/fisiopatologia , Nervo Tibial/patologia , Nervo Tibial/fisiopatologia , Adulto Jovem
7.
Beijing Da Xue Xue Bao Yi Xue Ban ; 47(2): 352-4, 2015 Apr 18.
Artigo em Chinês | MEDLINE | ID: mdl-25882960

RESUMO

A 36-year-old woman had an 8-year history of systemic lupus erythematosus (SLE) and was being treated with 10 mg/d of prednisone. She presented with a 6-month history of intermittent fever and multiple painful multi skin erythematous macules in her button, hips and extremities that had slowly enlarged to 8 cm×4 cm in diameter. The lesions started as painful erythematous macules, which eventually ulcerated and scared. Laboratory tests showed leukopenia, protenuria, positive anti-double strand DNA and hypocomplementemia. Cultures of the bottom ulcer were E. Coli, fugus and Tuberculous mycobacteria were both negative. Biopsy was performed and revealed necrosis of epidermis, thrombus and cellulose degeneration in epidermis with neutrophils karyorrhexis and vasculitis. Her SLE was active, so she was prescribed antibiotics for 2 weeks and prednisone was added to 60 mg/d for a month. However her skin ulcers did not relieve. When prednisone was added to 120 mg/d with combination therapy of cyclophosphamide and hydroxychloroquine, her skin ulcer cicatrized gradually.


Assuntos
Lúpus Eritematoso Sistêmico/diagnóstico , Paniculite/diagnóstico , Úlcera Cutânea/diagnóstico , Adulto , Ciclofosfamida , Feminino , Humanos , Prednisona , Úlcera Cutânea/tratamento farmacológico
8.
Eur Rev Med Pharmacol Sci ; 17(20): 2760-5, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24174357

RESUMO

AIM: The present study was conducted to investigate whether LBP had a protective effect on cerebral ischemic reperfusion injury and to determine the possible mechanisms. MATERIALS AND METHODS: Male Kunming (KM) mice were used to make the model cerebral artery occlusion/reperfusion (MCAO/R). The behavioral test was used to measure neurological deficit scores for evaluation of ischemic reperfusion damage of brain. The change of electroencephalograph (EEG) was monitored by Model SMUP-E Bio-electric Signals Processing System. The infarction area of brain was assessed in brain slices with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain. RESULTS: The results showed that LBP at doses of 20 and 40 mg/kg markedly decreased the neurological deficit scores and the infarction area in MCAO/R mice. At the same time, LBP significantly decreased MDA content, and increased SOD, GSH-Px, CAT, LDH activities in ischemic reperfusion brain. CONCLUSIONS: These suggest that LBP might act as a potential neuroprotective agent against the cerebral reperfusion-induced injury in the brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Fármacos Neuroprotetores/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Animais , Eletroencefalografia/efeitos dos fármacos , Masculino , Camundongos , Espécies Reativas de Oxigênio/metabolismo
9.
Plant Biol (Stuttg) ; 14(6): 914-22, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22672582

RESUMO

Rice Xa21 gene encodes a receptor-like kinase that confers broad-spectrum resistance against Xanthomonas oryzae pv. oryzae (Xoo). Recently, a number of genes involved in the Xa21-mediated disease resistance pathway have been identified. Based on our previous data and the literature, we chose 16 candidate proteins and made corresponding antibodies. Using Western blotting, we systematically investigated the expression profile of the proteins in Xa21-mediated disease resistance response. We found nine proteins with altered expression. We further compared their expression in resistance, susceptible and mock responses, and found that GST expression was up-regulated during the resistance process, indicating GST is a positive regulator in resistance responses. ATPsB expression was down-regulated during both the resistance and susceptible response processes, although it was higher in the resistance response than that in the susceptible response. The total amount of MYB, GAPDH, CatB, Trx and NB-ARC proteins was lower in the resistance than in the susceptible response, but their abundance per unit bacteria in the resistance response was still higher than in the susceptible response, suggesting that these proteins might be positive regulators in the resistance response. In addition, expression of another ERF was induced by inoculation with bacterial blight pathogen, and expression of Zf-LSD1 was activated by wounding stress alone. Interestingly, most proteins showed similar altered expression patterns in the resistance and susceptible responses, but differed to some extents, implying that both responses might share common molecular mechanisms. This study revealed evidence of resistance-related protein expression, providing a foundation for better understanding of their functions.


Assuntos
Resistência à Doença , Regulação da Expressão Gênica de Plantas , Oryza/imunologia , Proteínas de Plantas/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais , Anticorpos/imunologia , Antígenos de Plantas/imunologia , Western Blotting , Clonagem Molecular , Perfilação da Expressão Gênica/métodos , Genes de Plantas , Genes Reguladores , Glutationa Transferase/genética , Glutationa Transferase/imunologia , Glutationa Transferase/isolamento & purificação , Oryza/enzimologia , Oryza/metabolismo , Oryza/microbiologia , Fotossíntese , Doenças das Plantas/imunologia , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/imunologia , Espécies Reativas de Oxigênio/metabolismo , Estresse Fisiológico , Fatores de Transcrição/genética , Fatores de Transcrição/imunologia , Fatores de Transcrição/isolamento & purificação , Xanthomonas/patogenicidade
10.
Theriogenology ; 73(3): 383-91, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19945744

RESUMO

The use of ultrasonography as a noninvasive tool for assessing the reproductive status of the male Yangtze finless porpoise (YFP; Neophocaena phocaenoides asiaeorientalis) was validated by correlating ultrasonographically determined testicular volume (TV) and testicular parenchyma pixel intensity (PI) with serum testosterone (T) concentration. The testes of 13 free-ranging male YFPs from the Tian-e-Zhou Reserve and three captive animals from the Baiji Dolphinarium (Wuhan, China) were examined ultrasonographically during April 2008. Testis volume was determined using Lambert's formula for an ellipsoid. Testicular parenchyma PI was evaluated by analyzing testicular ultrasonograms using pixel analysis software (Image J). Serum T concentrations were determined using a single-antibody radioimmunoassay. The TV, PI, and serum T concentration were low and similar in animals with body length <133 cm, highest in those with body length >or=142 cm, and highly variable in those with body length from 133 to 141 cm. Both TV and PI were closely correlated with serum T concentration (r=0.91 and r=0.85, respectively; P<0.01), indicating a consistent association between structural and functional development of the testis. In conclusion, we inferred that puberty onset in male YFPs occurred when TV was >150 cm(3) and PI was >60 during the breeding season and that testicular ultrasonography and pixel analysis was an efficient, noninvasive, real-time tool to evaluate testicular function of live male YFPs.


Assuntos
Toninhas/fisiologia , Testículo/diagnóstico por imagem , Testosterona/sangue , Ultrassonografia/veterinária , Animais , Tamanho Corporal , Cruzamento , Masculino , Toninhas/anatomia & histologia , Toninhas/sangue , Estações do Ano , Testículo/crescimento & desenvolvimento
11.
Acta Biol Hung ; 59(3): 289-304, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18839696

RESUMO

This work describes the induction, purification and partial biochemical characterizations of an antimicrobial protein from the housefly larvae induced by ultrasonic wave. It has been purified to apparent homogeneity by ammonium sulfate precipitation followed by Sephadex G-75, Bio-gel P6 gel filtration, and CM-Sepharose Fast Flow cation exchange chromatography. The protein is a cationic protein with an apparent molecular weight of 16315 Da determined by no-denaturing electrophoresis and SDS-PAGE, respectively. Biochemical profile assays show that this protein has good thermal stability, and repeatedly frozen and defrosted durability. The optimum pH for antimicrobial activity is around pH5. The antimicrobial range of the protein includes Gram-positive, Gram-negative bacteria and some fungi. Results of the membrane permeability assays suggest that the probable mode of action of this protein is membrane-disrupting mechanism.


Assuntos
Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Moscas Domésticas/metabolismo , Proteínas de Insetos/isolamento & purificação , Proteínas de Insetos/farmacologia , Temperatura , Ultrassom , Fosfatase Alcalina/metabolismo , Animais , Escherichia coli/efeitos dos fármacos , Escherichia coli/enzimologia , Galactosidases/metabolismo , Concentração de Íons de Hidrogênio , Larva/metabolismo
12.
Acta Biol Hung ; 58(1): 75-86, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17385545

RESUMO

Chitosan was obtained from cuticles of the housefly (Musca domestica) larvae. Antibacterial activities of different Mw chitosans were examined against six bacteria. Antibacterial mechanisms of chitosan were investigated by measuring permeability of bacterial cell membranes and observing integrity of bacterial cells. Results show that the antibacterial activity of chitosan decreased with increase in Mw. Chitosan showed higher antibacterial activity at low pH. Ca2+ and Mg2+ could markedly reduce the antibacterial activity of chitosan. The minimum inhibitory concentrations of chitosans ranged from 0.03% - 0.25% and varied with the type of bacteria and Mw of chitosan. Chitosan could cause leakage of cell contents of the bacteria and disrupt the cell wall.


Assuntos
Antibacterianos/farmacologia , Quitosana/farmacologia , Moscas Domésticas/crescimento & desenvolvimento , Larva/fisiologia , Análise de Variância , Animais , Antibacterianos/isolamento & purificação , Quitosana/química , Quitosana/isolamento & purificação , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Peso Molecular , Viscosidade
13.
Nano Lett ; 7(2): 452-7, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17298014

RESUMO

Large strain plasticity is phenomenologically defined as the ability of a material to exhibit an exceptionally large deformation rate during mechanical deformation. It is a property that is well established for metals and alloys but is rarely observed for ceramic materials especially at low temperature ( approximately 300 K). With the reduction in dimensionality, however, unusual mechanical properties are shown by ceramic nanomaterials. In this Letter, we demonstrated unusually large strain plasticity of ceramic SiC nanowires (NWs) at temperatures close to room temperature that was directly observed in situ by a novel high-resolution transmission electron microscopy technique. The continuous plasticity of the SiC NWs is accompanied by a process of increased dislocation density at an early stage, followed by an obvious lattice distortion, and finally reaches an entire structure amorphization at the most strained region of the NW. These unusual phenomena for the SiC NWs are fundamentally important for understanding the nanoscale fracture and strain-induced band structure variation for high-temperature semiconductors. Our result may also provide useful information for further studying of nanoscale elastic-plastic and brittle-ductile transitions of ceramic materials with superplasticity.


Assuntos
Compostos Inorgânicos de Carbono/química , Cerâmica/química , Nanofios/química , Compostos de Silício/química , Elasticidade , Microscopia Eletrônica , Nanotecnologia , Nanofios/ultraestrutura , Análise Espectral , Estresse Mecânico , Temperatura
14.
Cryobiology ; 43(1): 46-53, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11812050

RESUMO

Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root was enhanced. Eight M26 single-bud sibling lines were used to assess genetic stability. Although cytological examination revealed a ploidy difference in the noncryopreserved control, the ploidy constitution remained relatively stable during the period of cryopreservation. Amplified fragment length polymorphism (AFLP) assay was performed to detect DNA-level variation. No change in DNA fragment pattern and number was observed between the control and the cryopreserved samples. In addition, methylation-sensitive amplified polymorphism (MSAP) assay was carried out to investigate the DNA methylation status during the period of cryopreservation. It was found that cryopreservation induced a decrease in DNA methylation level.


Assuntos
Criopreservação , Malus/genética , Sequência de Bases , Cromossomos/genética , Metilação de DNA , DNA de Plantas/química , DNA de Plantas/genética , Genes de Plantas , Malus/fisiologia , Biologia Molecular , Brotos de Planta/genética , Brotos de Planta/fisiologia , Ploidias , Polimorfismo Genético , Regeneração
15.
Gan To Kagaku Ryoho ; 27(9): 1403-9, 2000 Aug.
Artigo em Japonês | MEDLINE | ID: mdl-10969596

RESUMO

The expression of topoisomerase II alpha (Topo II alpha) was investigated in six human glioma cell lines as a function of doubling time, cell cycle distribution, and sensitivity to an antineoplastic agent, etoposide (ETP). The Topo II alpha level was determined by immunohistochemical and flow cytometric studies using monoclonal antibody, 8D2, specific for Topo II alpha. In all cell lines, the Topo II alpha level correlated directly with sensitivity to ETP, but not with doubling time or percentage of cells in any phase of the cell cycle. These findings suggest that the cytocidal activity of ETP may be mediated by qualitative and quantitative changes in Topo II alpha in human glioma cells. We speculate that better knowledge of Topo II alpha expression in surgical specimens may lead to more individualized use of ETP in patients with malignant glioma.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias Encefálicas/enzimologia , DNA Topoisomerases Tipo II/metabolismo , Etoposídeo/farmacologia , Glioma/enzimologia , Isoenzimas/metabolismo , Antígenos de Neoplasias , Neoplasias Encefálicas/patologia , Ciclo Celular , Divisão Celular , Proteínas de Ligação a DNA , Ensaios de Seleção de Medicamentos Antitumorais , Citometria de Fluxo , Glioma/patologia , Humanos , Imuno-Histoquímica , Células Tumorais Cultivadas/efeitos dos fármacos
16.
Shi Yan Sheng Wu Xue Bao ; 32(3): 289-95, 1999 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-12548811

RESUMO

Young ovules from 3 cultivars and undeveloped ovules in mature fruits from 8 cultivars of loose skin mandarin of Citrus were cultured on 4 different media respectively to induce embryogenic calli. Results showed that the combination of EME(MT + 500 mg/L malt extract) and MKT (EME + 10 mg/L KT) media performed well in the induction of embryogenic calli from young ovules; MGS(EME + 1 mg/L GA3 + 40 mg/L sulfate adenine) medium was better than MDB (MT + 0.01 mg/L 2,4-D + 0.1 mg/L BA) medium in inducing calli from the undeveloped ovules, and the darkness was conducive to the induction of embryogenic calli. There was no chromosome number variation in the induced calli. All of the examined cells were diploid with 2n = 2x = 18 chromosomes.


Assuntos
Citrus/crescimento & desenvolvimento , Técnicas de Cultura/métodos , Cromossomos/genética , Citrus/genética , Meios de Cultura/farmacologia , Variação Genética
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