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J Plant Physiol ; 264: 153487, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34358944


AtCYP38, a thylakoid lumen localized immunophilin, is found to be essential for photosystem II assembly and maintenance, but how AtCYP38 functions in chloroplast remains unknown. Based on previous functional studies and its crystal structure, we hypothesize that AtCYP38 should function via binding its targets or cofactors in the thylakoid lumen. To identify potential interacting proteins of AtCYP38, we first adopted ATTED-II and STRING web-tools, and found 12 proteins functionally related to AtCYP38. We then screened a yeast two-hybrid library including an Arabidopsis genome wide cDNA with different domain of AtCYP38, and five thylakoid lumen-localized targets were identified. In order to specifically search interacting proteins of AtCYP38 in the thylakoid lumen, we generated a yeast two-hybrid mini library including the thylakoid lumenal proteins and lumenal fractions of thylakoid membrane proteins, and we obtained six thylakoid membrane proteins and nine thylakoid lumenal proteins as interacting proteins of AtCYP38. The interactions between AtCYP38 and several potential targets were further confirmed via pull-down and co-immunoprecipitation assays. Together, a couple of new potential candidate interacting proteins of AtCYP38 were identified, and the results will lay a foundation for unveiling the regulatory mechanisms in photosynthesis by AtCYP38.

Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Cloroplastos/metabolismo , Ciclofilinas/metabolismo , Proteínas de Arabidopsis/fisiologia , Ciclofilinas/fisiologia , Imunoprecipitação , Complexo de Proteína do Fotossistema II/metabolismo , Domínios e Motivos de Interação entre Proteínas , Técnicas do Sistema de Duplo-Híbrido
Int J Mol Sci ; 22(13)2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34281206


The basic helix-loop-helix (bHLH) transcription factor family is one of the largest transcription factor gene families in Arabidopsis thaliana, and contains a bHLH motif that is highly conserved throughout eukaryotic organisms. Members of this family have two conserved motifs, a basic DNA binding region and a helix-loop-helix (HLH) region. These proteins containing bHLH domain usually act as homo- or heterodimers to regulate the expression of their target genes, which are involved in many physiological processes and have a broad range of functions in biosynthesis, metabolism and transduction of plant hormones. Although there are a number of articles on different aspects to provide detailed information on this family in plants, an overall summary is not available. In this review, we summarize various aspects of related studies that provide an overview of insights into the pleiotropic regulatory roles of these transcription factors in plant growth and development, stress response, biochemical functions and the web of signaling networks. We then provide an overview of the functional profile of the bHLH family and the regulatory mechanisms of other proteins.

Arabidopsis/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Sequência de Aminoácidos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Expressão Gênica , Genoma de Planta , Sequências Hélice-Alça-Hélice , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Análise de Sequência de DNA/métodos , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
PLoS One ; 11(10): e0165018, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27764209


The effect of cropping system on the distribution of organic carbon (OC) and nitrogen (N) in soil aggregates has not been well addressed, which is important for understanding the sequestration of OC and N in agricultural soils. We analyzed the distribution of OC and N associated with soil aggregates in three unfertilized cropping systems in a 27-year field experiment: continuously cropped alfalfa, continuously cropped wheat and a legume-grain rotation. The objectives were to understand the effect of cropping system on the distribution of OC and N in aggregates and to examine the relationships between the changes in OC and N stocks in total soils and in aggregates. The cropping systems increased the stocks of OC and N in total soils (0-40 cm) at mean rates of 15.6 g OC m-2 yr-1 and 1.2 g N m-2 yr-1 relative to a fallow control. The continuous cropping of alfalfa produced the largest increases at the 0-20 cm depth. The OC and N stocks in total soils were significantly correlated with the changes in the >0.053 mm aggregates. 27-year of cropping increased OC stocks in the >0.053 mm size class of aggregates and N stocks in the >0.25 mm size class but decreased OC stocks in the <0.053 mm size class and N stocks in the <0.25 mm size class. The increases in OC and N stocks in these aggregates accounted for 99.5 and 98.7% of the total increases, respectively, in the continuous alfalfa system. The increases in the OC and N stocks associated with the >0.25 mm aggregate size class accounted for more than 97% of the total increases in the continuous wheat and the legume-grain rotation systems. These results suggested that long-term cropping has the potential to sequester OC and N in soils and that the increases in soil OC and N stocks were mainly due to increases associated with aggregates >0.053 mm.

Agricultura/métodos , Carbono/análise , Produtos Agrícolas/crescimento & desenvolvimento , Nitrogênio/análise , Monitoramento Ambiental , Fabaceae/crescimento & desenvolvimento , Medicago sativa/crescimento & desenvolvimento , Solo/química , Triticum/crescimento & desenvolvimento
Mol Plant ; 5(3): 688-97, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22331621


Phytochromes (Phy) and phytochrome-interacting factor (PIF) transcription factors constitute a major signaling module that controls plant development in response to red and far-red light. A low red:far-red ratio is interpreted as shading by neighbor plants and induces cell elongation-a phenomenon called shade-avoidance syndrome (SAS). PAR1 and its closest homolog PAR2 are negative regulators of SAS; they belong to the HLH transcription factor family that lacks a typical basic domain required for DNA binding, and they are believed to regulate gene expressions through DNA binding transcription factors that are yet to be identified. Here, we show that light signal stabilizes PAR1 protein and PAR1 interacts with PIF4 and inhibits PIF4-mediated gene activation. DNA pull-down and chromatin immunoprecipitation (ChIP) assays showed that PAR1 inhibits PIF4 DNA binding in vitro and in vivo. Transgenic plants overexpressing PAR1 (PAR1OX) are insensitive to gibberellin (GA) or high temperature in hypocotyl elongation, similarly to the pifq mutant. In addition to PIF4, PAR1 also interacts with PRE1, a HLH transcription factor activated by brassinosteroid (BR) and GA. Overexpression of PRE1 largely suppressed the dwarf phenotype of PAR1OX. These results indicate that PAR1-PRE1 and PAR1-PIF4 heterodimers form a complex HLH/bHLH network regulating cell elongation and plant development in response to light and hormones.

Proteínas de Arabidopsis/administração & dosagem , Proteínas de Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/efeitos da radiação , Fatores de Transcrição Hélice-Alça-Hélice Básicos/administração & dosagem , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Luz , Fatores de Transcrição/metabolismo , Arabidopsis/citologia , Arabidopsis/genética , DNA de Plantas/metabolismo , Giberelinas/farmacologia , Hipocótilo/crescimento & desenvolvimento , Hipocótilo/metabolismo , Modelos Biológicos , Morfogênese/efeitos dos fármacos , Morfogênese/efeitos da radiação , Plantas Geneticamente Modificadas , Ligação Proteica/efeitos dos fármacos , Ligação Proteica/efeitos da radiação , Temperatura , Ativação Transcricional/efeitos dos fármacos , Ativação Transcricional/genética , Ativação Transcricional/efeitos da radiação
Dev Cell ; 19(6): 872-83, 2010 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-21145502


Light and brassinosteroid (BR) antagonistically regulate the developmental switch from etiolation in the dark to photomorphogenesis in the light in plants. Here, we identify GATA2 as a key transcriptional regulator that mediates the crosstalk between BR- and light-signaling pathways. Overexpression of GATA2 causes constitutive photomorphogenesis in the dark, whereas suppression of GATA2 reduces photomorphogenesis caused by light, BR deficiency, or the constitutive photomorphogenesis mutant cop1. Genome profiling and chromatin immunoprecipitation experiments show that GATA2 directly regulates genes that respond to both light and BR. BR represses GATA2 transcription through the BR-activated transcription factor BZR1, whereas light causes accumulation of GATA2 protein and feedback inhibition of GATA2 transcription. Dark-induced proteasomal degradation of GATA2 is dependent on the COP1 E3 ubiquitin ligase, and COP1 can ubiquitinate GATA2 in vitro. This study illustrates a molecular framework for antagonistic regulation of gene expression and seedling photomorphogenesis by BR and light.

Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Fator de Transcrição GATA2/metabolismo , Proteínas Nucleares/metabolismo , Arabidopsis/genética , Arabidopsis/efeitos da radiação , Sequência de Bases , Sítios de Ligação/genética , Primers do DNA/genética , Proteínas de Ligação a DNA , Fator de Transcrição GATA2/genética , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Luz , MicroRNAs/genética , Modelos Biológicos , Mutação , Processos Fototróficos/genética , Processos Fototróficos/fisiologia , Processos Fototróficos/efeitos da radiação , Reguladores de Crescimento de Plantas/metabolismo , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , RNA de Plantas/genética , Transdução de Sinais , Esteroides Heterocíclicos/metabolismo , Ubiquitina-Proteína Ligases/metabolismo