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1.
Biotechnol J ; : e2100183, 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34499787

RESUMO

AIM: This study aims to prepare green nanocomposite (HPMC/5-FL@GO) from the most biocompatible materials, hydroxypropyl methylcellulose (HPMC) and graphene oxide (GO), to enhance the drug activity of immobilized 5- Fluorouracil (5-FU) with decreasing the side effect of long-run treatment protocols with highly efficient drug-drug activity. METHOD AND RESULTS: Different samples were characterized by ATR-FTIR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive X-ray analysis (EDX), transmission electron microscopy (TEM), Thermogravimetric analysis (TGA), and dynamic light scattering (DLS) along with cytotoxicity and anticancer study. A homogenous and compatible nanocomposite structure with a homogenous drug distribution was confirmed. The results suggested that the prepared nanocomposite has a low cytotoxicity effect against normal Vero cell lines compared with 5-FU. The antitumor activities of the same nanocomposite (20.4 and 74.3 µg/ml on A549 and HepG-2) were lower than that of 5-FU (54.1and 103 µg/ml on A549 and HepG-2). CONCLUSION AND IMPLICATIONS: According to the attained results, the HPMC/5-FL@GO can be expected to be widely applied in a biomedical application such as cancer therapy with the unique biocompatible to human cells. This article is protected by copyright. All rights reserved.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34240303

RESUMO

Lithium-ion batteries and supercapacitors are examples of energy storage technologies that have a lot of promise in a variety of applications. Herein, NiO-porous carbon composites were prepared by a green and cost-effective facile synthesis route from banana peel waste materials. The surface morphology and chemical composition of the NiO-porous carbon composite were investigated using a scanning electron microscope (SEM) and energy dispersive x-ray analysis (EDX). The prepared samples were also described through Fourier transform infrared (FTIR) spectroscopy, x-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), and surface area measurements. The electrochemical behavior of prepared materials was studied by cyclic voltammetry (CV), galvanostatic charge-discharge, and electrochemical impedance (EIS) to test their applicable suitability as supercapacitor electrode. PC-NiO (3) composite exhibits a remarkable specific capacitance of 811 F/g at a current density of 1 A/g. The specific capacitance of PC-NiO (3) is 5.3 times more than that of PC material at 1.0 A/g. Furthermore, the PC-NiO (3) composite material still exhibits a specific capacitance of 780 F/g at 5.0 A/g, high rate capability of 84.55% retention at a high current density of 10.0 A/g and superior cycle stability at 1000 cycles. Based on its high specific capacitance, the NiO-porous carbon nanocomposite is one of the most promising electrode materials for supercapacitors, according to the above results.

3.
Molecules ; 26(12)2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34200763

RESUMO

The development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(m-hydroxyphenyl)porphyrin (mTHPP) loaded onto an ethylcellulose (EC)/chitosan (Chs) nanocomposite in eradicating multi-drug resistant Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. Surface loading of the ethylcelllose/chitosan composite with mTHPP was carried out and the resulting nanocomposite was fully characterized. The results indicate that the prepared nanocomposite incorporates mTHPP inside, and that the composite acquired an overall positive charge. The incorporation of mTHPP into the nanocomposite enhanced the photo- and thermal stability. Different laser wavelengths (458; 476; 488; 515; 635 nm), powers (5-70 mW), and exposure times (15-45 min) were investigated in the antimicrobial photodynamic therapy (aPDT) experiments, with the best inhibition observed using 635 nm with the mTHPP EC/Chs nanocomposite for C. albicans (59 ± 0.21%), P. aeruginosa (71.7 ± 1.72%), and S. aureus (74.2 ± 1.26%) with illumination of only 15 min. Utilization of higher doses (70 mW) for longer periods achieved more eradication of microbial growth.


Assuntos
Antibacterianos/química , Celulose/análogos & derivados , Quitosana/química , Nanocompostos/química , Porfirinas/química , Piridonas/química , Pirróis/química , Animais , Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Linhagem Celular , Celulose/química , Chlorocebus aethiops , Lasers , Luz , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/química , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Células Vero
4.
Int J Biol Macromol ; 161: 345-354, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32526303

RESUMO

There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications. Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin. Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques. Surface morphology of uninhibited and inhibited composites was studied using SEM and EDX. Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor. The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions. The data extracted from EIS were fitted through an equivalent circuit to model the corrosion inhibition.


Assuntos
Celulose/química , Cobre/química , Corrosão , Nanocompostos/química , Niacina/química , Cloreto de Sódio/química , Eletroquímica/métodos , Estrutura Molecular , Nanocompostos/ultraestrutura , Soluções , Análise Espectral
5.
Environ Sci Pollut Res Int ; 27(21): 26742-26749, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32378099

RESUMO

A new eco-friendly compound was prepared for the treatment of textile wastewater containing mixed dyes with various ranges of toxicity. Porous silica was extracted from a black liquor by-product using a simple method and characterized by porous morphology (the pore size ranged between 12 and 41 nm). The silica is the main corrosive agent present in the black liquor; thus, the extraction of silica from the black liquor was considered detoxification process. The extracted porous silica was used as a precursor material to prepare the hybrid material based on polyvinyl alcohol (PVA) as a binder polymer and functionalized by nicotinic acid. The multifunction prepared hybrid was characterized by FT-IR, TGA, DTGA, SEM, and EDX. The porous size of the prepared hybrid varied from 96 nm to 620 nm and presents a high thermal stability in comparison with its parent materials. The adsorption of cationic and anionic dyes was carried out. The adsorption kinetics parameters were fitted with pseudo-first-order and pseudo-second-order kinetic models for methyl orange (MO) and methylene blue (MB), respectively. The adsorption parameters indicated that the Langmuir model is better to describe the adsorption of dyes on the hybrid material. The maximum adsorption capacity was 484 and 771 mg/g for MO and MB, respectively.


Assuntos
Niacina , Oryza , Poluentes Químicos da Água/análise , Adsorção , Cinética , Álcool de Polivinil , Dióxido de Silício , Espectroscopia de Infravermelho com Transformada de Fourier , Têxteis , Águas Residuárias
6.
Int J Biol Macromol ; 144: 441-448, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31862374

RESUMO

The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents. The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively. The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11). The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity. The Trp-TOC showed slightly higher antimicrobial activity than Phe-TOC especially in time required of killing performance. The Phe-TOC has required 20 h for killing all microbial population while Trp-TOC required only 12 h. The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.


Assuntos
Anti-Infecciosos , Bactérias/crescimento & desenvolvimento , Candida albicans/crescimento & desenvolvimento , Celulose , Nanocompostos/química , Fenilalanina , Triptofano , Anti-Infecciosos/síntese química , Anti-Infecciosos/química , Anti-Infecciosos/farmacocinética , Celulose/química , Celulose/farmacologia , Fenilalanina/química , Fenilalanina/farmacologia , Triptofano/química , Triptofano/farmacologia
7.
J Microbiol Methods ; 168: 105802, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31809830

RESUMO

Watermelon peel waste (WPW) is a zero value material containing a high amount of nutrients that could be utilized in microbial growth medium production. In the present work, WPW was used to produce universal fungal growth medium with great nutritional profile to use instead of other expensive media particularly the Potato dextrose agar (PDA) medium. The mechanical and physical pretreatments were used to extract the growth medium from WPW. The main fiber composition was estimated as well as the elemental analysis and energy dispersive X-ray analysis (EDX) for the extracted medium. The total proteins, lipids and reducing sugars content were measured. The extracted WPW medium showed a high value of those macronutrients 9, 0.32 and 11 gl-1 respectively. The results of this study showed that mechanical pretreatment of WPW is the best for fungal growth; where the dry biomass obtained for Rhizopus oryzae, Lichtheimia corymbifera, Aspergillus niger, Penicillium expansium and Fusarium oxysporum were 1.53, 2.03, 2.37, 2.67 and 3.12 gl-1 respectively. Taguchi design was used to optimize the medium components. The optimum growth medium composition was prepared as follows; blending 500 gl-1 fresh WPW and 10 gl-1 of dextrose. Moreover dry biomass of R. oryzae on three liquid media namely watermelon peel waste dextrose (WPWD), Potato dextrose (PD) and Czapek,s dox (CzD) were 4.49, 4.16 and 2.39 respectively. Similarly, in the case of L. corymbifera, A. niger and P. expansium the dry biomass was increased on WPWD by 276, 267 and 250% respectively, while the dry biomass of F. oxysporum on WPWD was increased by 199%. Moreover, physicochemical properties of WPWDA medium are similar to PDA and CzDA. The formulated WPWDA medium is an alternative to commonly used media such as PDA and CzDA; and very effective for the growth of fungi, also this medium is eco-friendly and cheap.


Assuntos
Citrullus/química , Meios de Cultura/química , Fungos/crescimento & desenvolvimento , Resíduos , Biomassa , Fungos/classificação , Nutrientes/análise , Reciclagem
8.
Int J Biol Macromol ; 138: 975-985, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31351958

RESUMO

Despite the advantages of bacterial cellulose (BC) over traditional cellulose, its low yield and little bioactivity makes a limitation to be used in an industrial scale. This paper was mainly dual aimed to increase the BC yield using a nanobioactive glass (NBG), and in situ synthesize BC/NBG bioactive nanocomposites by a novel and simple green method. Accordingly, the composites were prepared via in situ fermentation approach by incorporation of NBG particles into BC producing culture medium. The effect of NBG addition on the production process of cellulose, biocompatibility, bioactivity and antimicrobial activity were investigated. The results showed that NBG was enhanced and increased the BC yield and this has been achieved by maintaining these NBG on the pH value of the culture medium during the fermentation period. Moreover, it was effectively improved biocompatibility and antimicrobial properties of BC. This study evidenced that BC/NBG composite can be expected to be widely applied in biomedical industries such as bone regeneration and wound healing with the unique of being not harmful to humans.


Assuntos
Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Celulose/química , Vidro/química , Nanocompostos/química , Polissacarídeos Bacterianos/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Celulose/síntese química , Técnicas de Química Sintética , Química Verde , Humanos , Polissacarídeos Bacterianos/síntese química , Análise Espectral
9.
Carbohydr Polym ; 211: 75-83, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30824106

RESUMO

Potato peel waste (PPW) is employed as the first report on bacterial cellulose (BC) production by Gluconacetobacter xylinus. Scharification of PPW was performed by 2 M different mineral acids individually. The suitable pre-treatment conditions were determined by reducing sugar release. Although all acid PPW-hydrolysates culture media are studied to produce BCs. Nitric acid hydrolysate gives the high productivity value The influence of nitric acid PPW-hydrolysate culture condition parameters were applied throughout the Taguchi method and the optimum conditions for the highest BC yield (4.7 g/L) was observed after 6 days at 35 °C, pH 9, medium volume 55 ml and with 8% inoculum size. The instrumental analysis of PPW-BC, included FT-IR, Particle size distribution, BET, DSC, XRD and SEM are cleared that the PPW-BC recorded high crystalliny82.5%, excellent PDI. In general, this study revealed that nitric acid PPW-hydrolysate could be used as cost effective alternative medium for production of BC with sustainable processes that can overcome the environmental pollution.


Assuntos
Celulose/metabolismo , Gluconacetobacter xylinus/metabolismo , Solanum tuberosum , Química Verde , Ácido Nítrico/química , Reciclagem , Resíduos
10.
Int J Biol Macromol ; 107(Pt A): 1364-1372, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29155155

RESUMO

This article deals with evaluating the role of cellulose origin, from wood and non-wood, on preparing green CMC-Ag complex as biological active agent. Viscose pulp as well as bagasse and rice straw pulps were used in preparation of CMCs, followed by complexation with AgNO3. The complex structure (free-Ag, IR-spectra and TGA), morphology (TEM), antibiological and anti-tumor activities were studied. The data revealed that, the main interaction between CMC and silver is occurred via carboxylate groups and ether link of 1ry alcohol, with formation stable 5-membered ring structure. For the case of RS-based CMC-Ag complex the interaction between COO groups and silica included RS is also possible, via hydrogen bonds. These complexes have anti-biological especially towards gram positive bacteria (B.subtilis, NCID-3610), and uni- and multi cellular fungi. AgNPs from viscose (VCMC-Ag complex) has relatively higher anti-tumor activity for breast cancer MCF-7 in vitro than bagasse-based CMC-Ag complex (BCMC-Ag complex) with IC50 128µg/ml (as Ag). It is interesting to note that; viscose-based CMC-Ag complex (VCMC-Ag) has higher efficient behaviour as bioactive agent than literature reported agents, e.g., Pyridine derivative (∼300µg/ml).


Assuntos
Antibacterianos/química , Carboximetilcelulose Sódica/química , Bactérias Gram-Positivas/efeitos dos fármacos , Substâncias Macromoleculares/química , Antibacterianos/síntese química , Antibacterianos/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Carboximetilcelulose Sódica/síntese química , Carboximetilcelulose Sódica/farmacologia , Proliferação de Células/efeitos dos fármacos , Celulose/química , Fungos/efeitos dos fármacos , Fungos/patogenicidade , Bactérias Gram-Positivas/patogenicidade , Química Verde , Humanos , Células MCF-7 , Substâncias Macromoleculares/síntese química , Substâncias Macromoleculares/farmacologia , Nanopartículas Metálicas/química , Prata/química
11.
Carbohydr Polym ; 153: 641-651, 2016 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-27561536

RESUMO

The present study deals with synthesizing novel cellulose derivative, from modifying the carboxymethyl cellulose with amino phenylpropanoic acid (CMC-APP). The synthesized CMC-APP was evaluated as biological and anti-cancer active compound. The molecular structures of this active compound were built using the HyperChem program 7.5, together with conventional analysis (nitrogen content, FT-IR, and non-isothermal TGA analysis). Optimizing the CMC/APPA ratio was carried out as preliminary assessment step, via undetected antimicrobial activity measurement. The TEM study showed that, the synthesized cellulose CMC-APP derivative in the nano-scale particle size (range from 12.5 to 89.3nm). Among all the tested microorganisms and MCF-7 breast cancer cells, the synthesized nano-cellulose derivative is possible used as safety medicine for microbial infections and cancers. The minimal inhibitory concentration (MIC) for Gram-positive bacteria, and gram-negative bacteria are 48.82µg/mL and 97µg/mL, respectively. While, the unicellular fungi and filamentous fungi are 12.2µg/mL and 97.65µg/mL, respectively. The cytotoxic index (IC50) for MCF-7 breast cancers is 50µg/mL. Moreover, the computational study of ADMET (absorption, distribution, metabolism, elimination and toxic) properties, of the molecules showed that, this investigated nano-compound is good oral bioavailability.


Assuntos
Antibacterianos/química , Antifúngicos/química , Antineoplásicos/química , Carboximetilcelulose Sódica/análogos & derivados , Fenilpropionatos/química , Aminação , Antibacterianos/síntese química , Antibacterianos/farmacologia , Antifúngicos/síntese química , Antifúngicos/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Carboximetilcelulose Sódica/síntese química , Carboximetilcelulose Sódica/farmacologia , Fungos/efeitos dos fármacos , Humanos , Células MCF-7 , Modelos Moleculares , Micoses/tratamento farmacológico , Fenilpropionatos/síntese química , Fenilpropionatos/farmacologia
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