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1.
Braz J Biol ; 83: e247701, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34468529

RESUMO

Staphylococcus aureus is an important foodborne pathogen associated to food intoxication and other multiple infections in human being. Its presence in salted food is a serious issue due to its salt tolerance potential. A study was conducted to analyze the presence of enterotoxins producing drug resistance S. aureus in salted sea fish from Gwadar. Freshly persevered samples (n=50) of salted fish were subjected to analyze the presence of S. aureus using 16S rRNA and Nuc genes primers. The isolates were then evaluated for drug resistance and enterotoxins producing potential using specific primers for MecA (methicillin resistance gene), (SEA) staphylococcal enterotoxin A and (SEB) staphylococcal enterotoxin B genes. Total 13/50 (26%) of the samples were found positive for the presence of S. aureus, preliminary confirmed with biochemical profiling and finally with the help of target genes presence. The isolates were found showing 100% resistant to methicillin, which were molecularly confirmed by the presence of MecA gene present in genome. The isolates 5/13 (38%) were positive for SEA and 3/13 (23%) for SEB genes, whereas 2/13 (15%) were confirmed having both SEA and SEB genes in its genome. It was also confirmed that all the isolates were capable to form biofilm over the glass surfaces. It was concluded that the study confirmed the presence of enterotoxigenic methicillin resistance Staphylococcus aurous (MRSA) in salted fish product, that poses gross food safety concern. Preventive and control measures are necessary to handle this serious food safety concern.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Animais , Produtos Pesqueiros , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , RNA Ribossômico 16S , Staphylococcus aureus/genética
2.
Leukemia ; 25(7): 1103-10, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21502954

RESUMO

Estrogen receptor ß (ERß) is expressed in immune cells and studies have suggested an antiproliferative function of ERß. We detected ERß expression in murine T- and human B-cell lymphoma cell lines and analyzed the effects of estradiol and selective ERß agonists on lymphoma growth in culture and in vivo. Treating the cells with estradiol had minor effects on cell growth, whereas the selective ERß agonists diarylpropionitrile (DPN) and KB9520 showed a strong antiproliferative effect. When grafting mice with murine T-cell lymphoma cells, male mice developed larger tumors compared with female mice, a difference that was abolished following ovariectomy, showing estrogen-dependent growth in vivo. To investigate whether lymphoma growth may be inhibited in vivo by ERß agonist treatment, mice grafted with murine lymphoma cells were treated with DPN or KB9520. Both ERß-selective agonists strongly inhibited lymphoma growth. The reduced tumor size seen following either DPN or KB9520 treatment was due to reduced proliferation and increased apoptosis. Our results show an ERß ligand-dependent antiproliferative effect of lymphoma cells expressing endogenous ERß and that lymphoma cell growth in vivo can efficiently be inhibited by ERß agonists. This suggests that ERß agonists may be useful in the treatment of lymphomas.


Assuntos
Antineoplásicos Hormonais/uso terapêutico , Linfoma de Burkitt/tratamento farmacológico , Receptor beta de Estrogênio/agonistas , Linfoma de Células T/tratamento farmacológico , Proteínas de Neoplasias/agonistas , Neoplasias Hormônio-Dependentes/tratamento farmacológico , Nitrilas/uso terapêutico , Propionatos/uso terapêutico , Animais , Antineoplásicos Hormonais/farmacologia , Apoptose/efeitos dos fármacos , Linfoma de Burkitt/patologia , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Estradiol/farmacologia , Receptor beta de Estrogênio/biossíntese , Receptor beta de Estrogênio/fisiologia , Feminino , Humanos , Neoplasias Hepáticas Experimentais/patologia , Linfoma de Células T/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteínas de Neoplasias/biossíntese , Proteínas de Neoplasias/fisiologia , Transplante de Neoplasias , Nitrilas/farmacologia , Ovariectomia , Propionatos/farmacologia , Especificidade da Espécie , Ensaios Antitumorais Modelo de Xenoenxerto
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