Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 272
Filtrar
1.
Neurosci Bull ; 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34611829

RESUMO

Deficits in the clearance of amyloid ß protein (Aß) by the peripheral system play a critical role in the pathogenesis of sporadic Alzheimer's disease (AD). Impaired uptake of Aß by dysfunctional monocytes is deemed to be one of the major mechanisms underlying deficient peripheral Aß clearance in AD. In the current study, flow cytometry and biochemical and behavioral techniques were applied to investigate the effects of polysaccharide krestin (PSK) on AD-related pathology in vitro and in vivo. We found that PSK, widely used in therapy for various cancers, has the potential to enhance Aß uptake and intracellular processing by human monocytes in vitro. After administration of PSK by intraperitoneal injection, APP/PS1 mice performed better in behavioral tests, along with reduced Aß deposition, neuroinflammation, neuronal loss, and tau hyperphosphorylation. These results suggest that PSK holds promise as a preventive agent for AD by strengthening the Aß clearance by blood monocytes and alleviating AD-like pathology.

2.
Water Res ; 206: 117724, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34637974

RESUMO

Algal bloom species can live upon internal regenerated ammonium (NH4+) for growth during the nitrogen-limited period. However, the linkages between NH4+ regeneration and phytoplankton biomass and community composition dynamics remain largely unknown. To unravel the interactions between NH4+ regeneration and phytoplankton community, we measured water column NH4+ regeneration rates (REGs) during a continuous phytoplankton growing period and a contrast summer/winter turnover in eutrophic Lake Taihu. Measured REGs were higher in summer than in winter and significantly correlated to total phytoplankton biomass, Cyanophyta biomass and its biomass proportions, and the concentrations of particulate nitrogen and dissolved organic carbon as well as the relative abundance of labile components (proteins and lipids). Random forest regression analyses displayed that variation of REGs were mainly controlled by water temperature and algal-related parameters (including chlorophyll a, total phytoplankton biomass, and Cyanophyta biomass). Partial least squares path model further revealed that algal-related parameters were the direct and significant factors regulating REGs, and contributed to the largest effect of the variance in REGs. Of the algal community, Cyanophyta was the dominant phylum to accelerate REGs. Correspondingly, rapid internal NH4+ turnover may strongly support the persistence of cyanobacterial blooms, thus forming a positive feedback between cyanobacterial blooms and REGs during the nitrogen-limited summer months. We therefore deduced that the internal loop between cyanobacterial blooms and REGs during summer may be a key self-maintenance mechanism of continuous cyanobacterial blooms.

3.
BMJ ; 375: n2183, 2021 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-34610915

RESUMO

OBJECTIVE: To update a previous individual participant data meta-analysis and determine the accuracy of the Patient Health Questionnaire-9 (PHQ-9), the most commonly used depression screening tool in general practice, for detecting major depression overall and by study or participant subgroups. DESIGN: Systematic review and individual participant data meta-analysis. DATA SOURCES: Medline, Medline In-Process, and Other Non-Indexed Citations via Ovid, PsycINFO, Web of Science searched through 9 May 2018. REVIEW METHODS: Eligible studies administered the PHQ-9 and classified current major depression status using a validated semistructured diagnostic interview (designed for clinician administration), fully structured interview (designed for lay administration), or the Mini International Neuropsychiatric Interview (MINI; a brief interview designed for lay administration). A bivariate random effects meta-analytic model was used to obtain point and interval estimates of pooled PHQ-9 sensitivity and specificity at cut-off values 5-15, separately, among studies that used semistructured diagnostic interviews (eg, Structured Clinical Interview for Diagnostic and Statistical Manual), fully structured interviews (eg, Composite International Diagnostic Interview), and the MINI. Meta-regression was used to investigate whether PHQ-9 accuracy correlated with reference standard categories and participant characteristics. RESULTS: Data from 44 503 total participants (27 146 additional from the update) were obtained from 100 of 127 eligible studies (42 additional studies; 79% eligible studies; 86% eligible participants). Among studies with a semistructured interview reference standard, pooled PHQ-9 sensitivity and specificity (95% confidence interval) at the standard cut-off value of ≥10, which maximised combined sensitivity and specificity, were 0.85 (0.79 to 0.89) and 0.85 (0.82 to 0.87), respectively. Specificity was similar across reference standards, but sensitivity in studies with semistructured interviews was 7-24% (median 21%) higher than with fully structured reference standards and 2-14% (median 11%) higher than with the MINI across cut-off values. Across reference standards and cut-off values, specificity was 0-10% (median 3%) higher for men and 0-12 (median 5%) higher for people aged 60 or older. CONCLUSIONS: Researchers and clinicians could use results to determine outcomes, such as total number of positive screens and false positive screens, at different PHQ-9 cut-off values for different clinical settings using the knowledge translation tool at www.depressionscreening100.com/phq. STUDY REGISTRATION: PROSPERO CRD42014010673.

4.
Front Cardiovasc Med ; 8: 720857, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34646872

RESUMO

Background: Contrast-associated acute kidney injury (CA-AKI) is responsible for a substantial proportion of the observed mortality that occurs after percutaneous coronary intervention (PCI), particularly in elderly patients. However, there has been significant and debate over whether the optimal definition of CA-AKI persists over prolonged periods due to variations in the prevalence and effects on prognosis. In this study, we aimed to identify whether different definitions of CA-AKI exert differential impacts on long-term mortality when compared between elderly and non-elderly patients receiving elective PCI. Methods: We prospectively investigated 5,587 consenting patients undergoing elective PCI between January 2012 and December 2018. We considered two classical definitions of CA-AKI from the European Society of Urogenital Radiology (ESUR) and the Acute Kidney Injury Network (AKIN). Multivariable Cox regression analysis was used to investigate the association between CA-AKI and long-term mortality. We also performed interaction and stratified analyses according to age (≤75 or >75 years). Results: The incidence of CA-AKI according to the ESUR and AKIN definitions was 18.7 and 6.1%, respectively. After a median follow-up of 2.1 years, multivariable Cox regression analysis indicated that CA-AKI according to the AKIN definition was a risk factor for long-term mortality in the overall population [hazard ratio (HR) = 2.20; 95% confidential interval (CI): 1.51-3.22; p < 0.001]; however, this was not the case for the ESUR definition (HR = 1.27; 95% CI: 0.92-1.76; p = 0.153). Further interaction analysis identified a significant interaction between age and the ESUR definition (p = 0.040). Stratified analyses also found an association between the ESUR definition and long-term mortality in patients >75 years of age (p = 0.011), but not in patients ≤75 years of age (p = 0.657). Conclusion: As a stringent definition of CA-AKI, the AKIN definition was significantly associated with long-term mortality in both non-elderly and elderly patients. However, in elderly patients, the more lenient definition provided by the ESUR was also significantly correlated with long-term mortality, which could sensitively identify high-risk elderly patients and may provide a better alternative.

5.
Environ Sci Process Impacts ; 23(10): 1466-1475, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34669760

RESUMO

Dissolved organic matter (DOM) in petroleum refinery wastewater is an extremely complex mixture. A better understanding of chemical compositions of DOM at the molecular level is necessary for the design and optimization of wastewater treatment processes. In this study, two largely different DOM samples, one from a petroleum refinery wastewater and the other from the Suwannee river water, were characterized by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) coupled with positive-/negative-ion electrospray ionization (ESI), and positive-ion atmospheric pressure photoionization (APPI). For wastewater DOM, a total of 6226 molecular formulae were assigned in the three ionization modes. However, only 1182 molecular formulae were common in all three mass spectra, indicating that the techniques were highly complementary in the types of molecules they ionize. Acid Ox (x = 1-9) and basic N1Ox (x = 0-2) classes were dominant in the wastewater DOM detected in negative-ion and positive-ion ESI mode, respectively. And the wastewater DOM contains considerable amounts of polycyclic aromatic hydrocarbons that did not respond to ESI but can be ionized selectively by APPI. Compared with riverine DOM, the refinery wastewater DOM has a higher molecular complexity and is more enriched in hydrocarbon, and nitrogen- and sulfur-containing compounds. The results show that the major components of refinery wastewater DOM were distinctive from those of the natural organic matter. Though not quantitative, the results obtained by various ionization techniques were found to be complementary, and are helpful to our understanding of the selectivity of different ionization techniques as well as the molecular compositions of DOM.


Assuntos
Petróleo , Águas Residuárias , Pressão Atmosférica , Espectrometria de Massas , Nitrogênio
6.
Microbiol Spectr ; : e0153121, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34668747

RESUMO

The microbial carbon pump (MCP) provides a mechanistic illustration of transformation of recalcitrant dissolved organic matter (DOM) in the ocean. Here, we explored and demonstrated the key roles of algae-associated microorganisms (mainly heterotrophic bacteria) in the production and transformation of carboxyl-rich alicyclic molecule (CRAM)-like DOM through a laboratory experiment involving cultures of Skeletonema dohrnii. Without the participation of the associated bacteria, CRAM-like DOM molecules were not detected via Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in algal cultures treated with antibiotics. Similarly, CRAM-like DOM were not detected in cultures of bacteria alone. Our experimental results showed that algae-associated bacteria are important in the process of converting algal-derived organic matter into CRAM-like DOM during S. dohrnii culture. Bacteroidetes (mainly Flavobacteriia) dominated the bacterial community in the stationary and degradation phases, where the predicted metabolic pathways for bacterial assemblages were mainly involved in biosynthesis, metabolism, and degradation. Facilitated by these heterotrophic bacteria, the amount and the chemodiversity of CRAM-like DOM derived from algae varied during the growth and decomposition of algal cells, and CRAM-like DOM were enriched at the later growth stage. The properties and characteristics of these CRAM-like DOM, including molecular weight, double bond equivalent, hydrogen-carbon ratio, carbon-nitrogen ratio, carbon-sulfur ratio, and modified aromaticity index increased with the growth and decay of algal cells, indicating the transformation from active to recalcitrant DOM. In contrast, the organic matter in axenic cultures of S. dohrnii mainly existed in the form of particulate organic matters (POM), and small amounts of CRAM-like DOM were detected. This study provides the first laboratory evidence to reveal and confirm the direct involvement of algae-associated microbiomes in the production and transformation of algae-derived refractory DOM, highlighting the significance of these epiphytic bacteria in marine carbon sequestration and global carbon cycling. IMPORTANCE Dissolved organic matter (DOM) serves as a major carbon and nutrient pool in oceans, and recalcitrant DOM are the primary sources for carbon sequestration in depths. Here, we demonstrate the critical roles of algae-associated microorganisms (mainly heterotrophic bacteria) in the transformation of recalcitrant dissolved organic matter through laboratory cultures of a model diatom, Skeletonema dohrnii. Our experimental results showed that in addition to affecting the growth and the physiology of S. dohrnii, algae-associated bacteria are important in processing and converting algal DOM into CRAM-like DOM. Facilitated by the associated bacteria, the amount and the chemodiversity of DOM derived from algae varied during the growth and decomposition of algal cells, and enriched recalcitrant DOM formed in the later growth stage. The properties and diversity of DOM increased with the growth and decay of algal cells, indicating the transformation from active DOM to inert organic matter. Our results confirmed that the direct involvement of algae-associated microbes in the production of CRAM-like DOM. Detailed community structure analysis of the algae-associated bacterial community and its predicted functions confirmed the involvement of certain bacterial groups (e.g., Flavobacteriia) in biosynthesis, metabolism, and degradation.

7.
Cell Metab ; 33(10): 1957-1973.e6, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34614408

RESUMO

Skeletal aging is characterized by low bone turnover and marrow fat accumulation. However, the underlying mechanism for this imbalance is unclear. Here, we show that during aging in rats and mice proinflammatory and senescent subtypes of immune cells, including macrophages and neutrophils, accumulate in the bone marrow and secrete abundant grancalcin. The injection of recombinant grancalcin into young mice was sufficient to induce premature skeletal aging. In contrast, genetic deletion of Gca in neutrophils and macrophages delayed skeletal aging. Mechanistically, we found that grancalcin binds to the plexin-b2 receptor and partially inactivates its downstream signaling pathways, thus repressing osteogenesis and promoting adipogenesis of bone marrow mesenchymal stromal cells. Heterozygous genetic deletion of Plexnb2 in skeletal stem cells abrogated the improved bone phenotype of Gca-knockout mice. Finally, we developed a grancalcin-neutralizing antibody and showed that its treatment of older mice improved bone health. Together, our data suggest that grancalcin could be a potential target for the treatment of age-related osteoporosis.

8.
J Cardiol ; 2021 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-34551865

RESUMO

BACKGROUND: Neutrophil and albumin are well-known biomarkers of inflammation, which are highly related to contrast-associated acute kidney injury (CA-AKI). We aim to explore the predictive value of neutrophil percentage-to-albumin ratio (NPAR) for CA-AKI and long-term mortality in patients without chronic kidney disease (CKD) undergoing elective percutaneous coronary intervention (PCI). METHODS: We retrospectively observed 5083 consenting patients from January 2012 to December 2018. CA-AKI was defined as an increase in serum creatinine ≥50% or 0.3 mg/dL within 48 h after contrast medium exposure. RESULTS: The incidence of CA-AKI was 5.6% (n=286). The optimal cut-off value of NPAR for predicting CA-AKI was 15.7 with 66.8% sensitivity and 61.9% specificity [C statistic=0.679; 95% confidence interval (CI), 0.666-0.691]. NPAR displayed higher area under the curve values in comparison to neutrophil percentage (p < 0.001) and neutrophil-to-albumin ratio (NAR) (p < 0.001), but not albumin (p = 0.063). However, NPAR significantly improved the prediction of CA-AKI assessed by the continuous net reclassification improvement (NRI) and integrated discrimination improvement (IDI) compared to neutrophil percentage (NRI=0.353, 95% CI: 0.234-0.472, p < 0.001; IDI=0.017, 95% CI: 0.010-0.024, p < 0.001) and albumin (NRI=0.141, 95% CI: 0.022-0.260, p = 0.020; IDI=0.009, 95% CI: 0.003-0.015, p = 0.003) alone. After adjusting for potential confounding factors, multivariate analysis showed that NPAR >15.7 was a strong independent predictor of CA-AKI (odds ratio =1.90, 95% CI: 1.38-2.63, p < 0.001). Additionally, NPAR >15.7 was significantly associated with long-term mortality during a median of 2.9 years of follow-up (hazard ratio =1.68, 95% CI: 1.32-2.13; p < 0.001). CONCLUSIONS: NPAR was an independent predictor of CA-AKI and long-term mortality in patients without CKD undergoing elective PCI.

9.
Water Res ; 204: 117609, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34509866

RESUMO

Reservoirs have boomed for clean energy in recent decades and interrupted the natural river ecosystem severely. Riverine dissolved organic matter (DOM), which regulates aquatic food web dynamics, water quality, and carbon storage, has been significantly impacted by reservoir construction. However, the vertical evolution of DOM properties and its controlling mechanisms in large reservoirs with hydrological management are not well investigated, limiting the understanding of carbon cycling (e.g., CO2 emissions and carbon burial) in reservoirs. To fill this knowledge gap, multiple complementary techniques including optical spectroscopy and ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry were applied to track composition and property changes of DOM along the vertical profile in a large deep tributary of the world largest Three Gorges Reservoir (TGR) during the water-level rising period. The results indicated that middle and bottom water have relatively more terrestrial input and recalcitrant DOM, while surface water has relatively more autochthonous input and labile DOM. Integrated with the comprehensive analysis of DOM chemistry in a high-resolution vertical profile, the primary production and photodegradation in surface water, the density currents induced water intrusion from mainstream to tributaries, in middle water, and the biodegradation in bottom water are main factors controlling the vertical heterogeneity of reservoir DOM during the water-level rising period. This vertical increase of DOM recalcitrance likely contributes to the enhancement of organic carbon burial in TGR during the water-level rising period. All in all, this study provides new insight into the vertical variations of riverine DOM induced by reservoir construction, and emphasizes the important role of reservoir construction in carbon sequestration.


Assuntos
Ecossistema , Água , Hidrologia , Rios , Qualidade da Água
10.
J Chem Phys ; 155(9): 094305, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34496579

RESUMO

DNA strands are polymeric ligands that both protect and tune molecular-sized silver cluster chromophores. We studied single-stranded DNA C4AC4TC3XT4 with X = guanosine and inosine that form a green fluorescent Ag10 6+ cluster, but these two hosts are distinguished by their binding sites and the brightness of their Ag10 6+ adducts. The nucleobase subunits in these oligomers collectively coordinate this cluster, and fs time-resolved infrared spectra previously identified one point of contact between the C2-NH2 of the X = guanosine, an interaction that is precluded for inosine. Furthermore, this single nucleobase controls the cluster fluorescence as the X = guanosine complex is ∼2.5× dimmer. We discuss the electronic relaxation in these two complexes using transient absorption spectroscopy in the time window 200 fs-400 µs. Three prominent features emerged: a ground state bleach, an excited state absorption, and a stimulated emission. Stimulated emission at the earliest delay time (200 fs) suggests that the emissive state is populated promptly following photoexcitation. Concurrently, the excited state decays and the ground state recovers, and these changes are ∼2× faster for the X = guanosine compared to the X = inosine cluster, paralleling their brightness difference. In contrast to similar radiative decay rates, the nonradiative decay rate is 7× higher with the X = guanosine vs inosine strand. A minor decay channel via a dark state is discussed. The possible correlation between the nonradiative decay and selective coordination with the X = guanosine/inosine suggests that specific nucleobase subunits within a DNA strand can modulate cluster-ligand interactions and, in turn, cluster brightness.


Assuntos
DNA de Cadeia Simples/química , Guanosina/química , Inosina/química , Prata/química , Sítios de Ligação , Fluorescência
11.
Front Microbiol ; 12: 697860, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34354693

RESUMO

Dissolved organic matter (DOM) provides carbon substrates and energy sources for sediment microbes driving benthic biogeochemical processes. The interactions between microbes and DOM are dynamic and complex and require the understanding based on fine-scale microbial community and physicochemical profiling. In this study, we characterized the porewater DOM composition in a 300-cm sediment core from the Pearl River estuary, China, and examined the interactions between DOM and archaeal and bacterial communities. DOM composition were highly stratified and associated with changing microbial communities. Compared to bacteria, the amplicon sequence variants of archaea showed significant Pearson correlations (r ≥ 0.65, P < 0.01) with DOM molecules of low H/C ratios, high C number and double bond equivalents, indicating that the distribution of archaea was closely correlated to recalcitrant DOM while bacteria were associated with relatively labile compounds. This was supported by the presence of auxiliary enzyme families essential for lignin degradation and bcrABCD, UbiX genes for anaerobic aromatic reduction in metagenome-assembled genomes of Bathyarchaeia. Our study demonstrates that niche differentiation between benthic bacteria and archaea may have important consequences in carbon metabolism, particularly for the transformation of recalcitrant organic carbon that may be predominant in aged marine sediments.

12.
Pain Ther ; 2021 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-34278548

RESUMO

Stroke is a leading cause of death worldwide, and about a quarter of stroke patients are dead within 1 month. The prognosis is even worse for those with hemorrhagic stroke because the 1-month mortality approaches 50%. Besides, most patients who survive experience complications such as nausea, vomiting, and chronic pain. These adverse experiences, especially the existence of chronic pain, can lead to a decline in the patient's quality of life. In order to improve the treatment and prognosis of hemorrhagic stroke, there is an urgent need to understand its pathophysiological mechanism as well as the chronic pain it induces. This paper reviews studies of the molecular mechanisms of hemorrhagic stroke, especially the activation of microglia and the relationship between microglia and pain after stroke, which could shed new light on hemorrhagic stroke treatment.

13.
Nat Commun ; 12(1): 4089, 2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-34215733

RESUMO

Pediatric high-grade glioma (pHGG) is a major contributor to cancer-related death in children. In vitro and in vivo disease models reflecting the intimate connection between developmental context and pathogenesis of pHGG are essential to advance understanding and identify therapeutic vulnerabilities. Here we report establishment of 21 patient-derived pHGG orthotopic xenograft (PDOX) models and eight matched cell lines from diverse groups of pHGG. These models recapitulate histopathology, DNA methylation signatures, mutations and gene expression patterns of the patient tumors from which they were derived, and include rare subgroups not well-represented by existing models. We deploy 16 new and existing cell lines for high-throughput screening (HTS). In vitro HTS results predict variable in vivo response to PI3K/mTOR and MEK pathway inhibitors. These unique new models and an online interactive data portal for exploration of associated detailed molecular characterization and HTS chemical sensitivity data provide a rich resource for pediatric brain tumor research.


Assuntos
Heterogeneidade Genética/efeitos dos fármacos , Glioma/tratamento farmacológico , Glioma/genética , Animais , Neoplasias Encefálicas , Linhagem Celular Tumoral , Proliferação de Células , Criança , Modelos Animais de Doenças , Regulação Neoplásica da Expressão Gênica , Glioma/patologia , Ensaios de Triagem em Larga Escala , Humanos , Camundongos , Mutação , Inibidores de Proteínas Quinases/uso terapêutico , Serina-Treonina Quinases TOR , Ensaios Antitumorais Modelo de Xenoenxerto
14.
Zool Res ; 42(4): 514-524, 2021 07 18.
Artigo em Inglês | MEDLINE | ID: mdl-34254745

RESUMO

Normal spermatogenic processes require the scrotal temperature to be lower than that of the body as excessive heat affects spermatogenesis in the testes, reduces sperm quality and quantity, and even causes infertility. Endoplasmic reticulum stress (ERS) is a crucial factor in many pathologies. Although several studies have linked ERS to heat stress, researchers have not yet determined which ERS signaling pathways contribute to heat-induced testicular damage. Melatonin activates antioxidant enzymes, scavenges free radicals, and protects the testes from inflammation; however, few studies have reported on the influence of melatonin on heat-induced testicular damage. Using a murine model of testicular hyperthermia, we observed that heat stress causes both ERS and apoptosis in the testes, especially in the spermatocytes. These observations were confirmed using the mouse spermatocyte cell line GC2, where the Atf6 and Perk signaling pathways were activated during heat stress. Knockout of the above genes effectively reduced spermatocyte damage caused by heat stress. Pretreatment with melatonin alleviated heat-induced apoptosis by inhibiting the Atf6 and Perk signaling pathways. This mitigation was dependent on the melatonin receptors. In vivo experiments verified that melatonin treatment relieved heat-induced testicular damage. In conclusion, our results demonstrated that ATF6 and PERK are important mediators for heat-induced apoptosis, which can be prevented by melatonin treatment. Thus, our study highlights melatonin as a potential therapeutic agent in mammals for subfertility/infertility induced by testicular hyperthermia.


Assuntos
Fator 6 Ativador da Transcrição/metabolismo , Temperatura Alta/efeitos adversos , Melatonina/farmacologia , Espermatócitos/efeitos dos fármacos , Testículo/fisiologia , eIF-2 Quinase/metabolismo , Fator 6 Ativador da Transcrição/genética , Animais , Apoptose/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Transdução de Sinais/efeitos dos fármacos , eIF-2 Quinase/genética
15.
Chemosphere ; 286(Pt 1): 131617, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34303906

RESUMO

In petroleum refineries, the electric desalting, distillation, and stripping processes could generate large amounts of wastewaters that contain toxic substances. In this study, eight wastewater samples were collected from the three typical refining processes for comprehensive chemical characterization of the dissolved organic matter (DOM) using excitation emission matrix fluorescence spectroscopy, gas chromatography-mass spectrometry, and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Results showed that protein-like components and benzene were ubiquitous in all these wastewaters. Oxygen-containing volatile organic compounds had higher contents in crude distillation and stripping wastewater than those in electric desalting wastewater. Among the three refinery processes, molecular composition of DOM in the stripping wastewater had the highest complexity. The Ox and OxSy class species assigned from the negative-ion electrospray ionization FT-ICR MS were dominant in all wastewaters. The OxS2 class species which were effectively removed during stripping treatment had highest relative abundance in stripping influent. These results are instructive to guide the development of "divide and conquer" and would improve the treatment and management of refinery wastewater streams.

16.
Phytochemistry ; 190: 112893, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34332297

RESUMO

Four previously undescribed iridoid glycosides, including two bis-iridoid glycosides, and three undescribed lignans, together with 16 known analogues, were isolated from the fruits of Gardenia jasminoides Eills. Their structures were elucidated on basis of spectroscopic methods, and the absolute configurations of three of the unknown compounds were determined by interpretation of their electronic circular dichroic (ECD) and [α] [Formula: see text] data. The α-glucosidase inhibitory effects of the isolated compounds were evaluated and all the compounds exhibited slightly inhibitory activity with the values of IC50 greater than 50 µM.


Assuntos
Gardenia , Lignanas , Frutas , Glicosídeos/farmacologia , Glicosídeos Iridoides/farmacologia , Lignanas/farmacologia , Extratos Vegetais
17.
Water Res ; 201: 117358, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34175728

RESUMO

Dissolved organic matter (DOM) chemistry in rivers regulates aquatic food web dynamics, water quality, and carbon storage. The operation of reservoirs represents one of the major human modifications on the natural flow of rivers, which can affect DOM chemistry. Although hydrologic heterogeneity has been observed in different segments of the reservoir, whether it will structure DOM chemistry is poorly assessed, which is critical to better constrain the carbon cycle in reservoirs. By the combination of a series of techniques including stable carbon isotopes, optical spectroscopy, and ultrahigh-resolution mass spectrometry, here we showed that hydrologic heterogeneity induced changes in DOM molecular composition between two large tributaries, named Shennongxi (SR) and Xiangxi (XR) rivers, of the Three Gorges Reservoir (TGR). With water intrusion from mainstream, SR had relatively higher terrestrial and more recalcitrant DOM than XR, where no water intrusion from the mainstream was observed. In contrast, the averaged relative abundance of autochthonous input and biological lability of DOM along upstream to downstream transect were higher in XR than that in SR. The presence or absence of water intrusion from mainstream to tributaries induced by hydrologic management is likely the main factor controlling DOM chemistry in different tributaries of the TGR. By linking DOM chemistry in the water column with that in surface sediments, we suggest that hydrologic management of reservoir likely affects the preferential preservation of recalcitrant DOM in surface sediments, which further affects the organic carbon burial and the river carbon cycle. With reservoir construction increasing worldwide, further studies are encouraged to investigate the DOM chemistry under different hydrologic management of reservoirs to better constrain and predict the carbon cycling in fluvial ecosystems.


Assuntos
Ecossistema , Hidrologia , Carbono , Humanos , Rios , Qualidade da Água
18.
BMC Cancer ; 21(1): 739, 2021 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-34176477

RESUMO

BACKGROUND: The health burden of breast cancer is rising in China. The effect of informed diagnosis on long-term survival is not fully understood. This retrospective cohort study aims to explore the association between early informed diagnosis and survival time in breast cancer patients. METHODS: A total of 12,327 breast cancer patients were enrolled between October 2002 and December 2016. Potential factors, including knowing the cancer diagnosis status, sex, age, clinical stage, surgery history, grade of reporting hospital and diagnostic year were, analyzed. We followed up all participants every 6 months until June 2017. Propensity score matching (PSM) was used to balance the clinicopathologic characteristics between patients who knew their diagnosis and those who did not. RESULTS: By June 2017, 18.04% of the participants died of breast cancer. Before PSM, both the 3-year and 5-year survival rates of patients who knew their cancer diagnosis were longer (P < 0.001). After PSM, the above conclusion was still established. By stratified analysis, except for the subgroups of male patients and stage III patients, patients who knew their diagnosis showed a better prognosis in all the other subgroups (P < 0.05). Cox regression analysis showed that knowing a cancer diagnosis was an independent risk factor for survival in breast cancer patients (P < 0.001). CONCLUSIONS: Being aware of their cancer diagnosis plays a protective role in extending the survival time of breast cancer patients, which suggests that medical staff and patients' families should disclose the cancer diagnosis to patients in a timely manner. Further prospective studies need to be made to validate our findings.


Assuntos
Neoplasias da Mama/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Mama/mortalidade , Neoplasias da Mama/patologia , Feminino , Seguimentos , Humanos , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Análise de Sobrevida
19.
Sci Total Environ ; 784: 147095, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34088033

RESUMO

As one of the most dynamic and reactive compound pools, DOM plays a crucial part in various biochemical processes such as element cycling and nutrient export. Although the reservoir DOM has been investigated extensively, the variation of DOM between reservoir area and non-reservoir area induced by reservoir construction is not comprehensively assessed. By the combination of a series of complementary techniques including stable carbon isotope, optical spectroscopy, and ultrahigh-resolution mass spectrometry, here we show that hydrological alterations induced by Three Gorges Reservoir (TGR) construction were responsible for the variation in DOM molecular composition between reservoir area and non-reservoir area in Xiangxi tributary. With water intrusion from mainstream induced by reservoir construction and operation, reservoir area had relatively higher terrestrial input and more recalcitrant DOM than those in the non-reservoir area with limited influence of reservoir operation, whereas, relatively more autochthonous source and higher molecular lability of DOM were observed in the non-reservoir area. The water intrusion from mainstream to tributaries induced by reservoir construction is likely the main factor controlling DOM variation between reservoir area and non-reservoir area, and might devote to the organic carbon burial in the reservoir. This research provides new insight into spatial variations of riverine DOM composition induced by reservoir construction, underlining the important role of the reservoir in DOM cycling.

20.
Nat Prod Res ; : 1-7, 2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34100675

RESUMO

A new quinic acid derivative (1) along with seven known compounds (2-8) were isolated from the fruit of Gardenia jasminoides J.Ellis. All the structures of the isolated compounds were elucidated on the basis of the analysis of multiple spectroscopic data. The α-glucosidase inhibitory effects of the isolated compounds were investigated. The results showed that compounds 1-3, 6, and 8 have moderate α-glucosidase inhibitory activities.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...