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1.
Glycoconj J ; 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32103424

RESUMO

Currently, the definitive diagnosis in breast cancer requires biopsy and histopathology, such the most effective markers are tissue-based. However, the advantages of saliva in collection and storage make it possible for assessing human pathology and contributing to the development of cancer-related biomarkers for clinical application. The present study validated alteration of salivary protein glycopatterns recognized by Bandeiraea simplicifolia lectin I (BS-I) in the saliva of patients with breast diseases using saliva microarrays, and the N/O-glycan profiles of their salivary glycoproteins isolated by the BS-I-magnetic particle conjugates from 259 female subjects (66 healthy volunteers (HV), 65 benign breast cyst or tumor patients (BB), 66 patients with breast cancer in stage I (BC-I) and 62 patients with breast cancer in stage II (BC-II)) were analyzed by MALDI-TOF/TOF-MS. The results showed that the expression level of galactosylated glycans recognized by BS-I was significantly increased in patients with breast cancer compared with HV (p < 0.05). Totally, there were 11/10, 10/19, 7/24 and 7/9 galactosylated N-/O-linked glycans were identified and annotated from the pooled salivary samples of HV, BB, BC-I and BC-II, respectively. One galactosylated N-glycan peak (m/z 2773.977), and 4 galactosylated O-glycan peaks (m/z 868.295, 882.243, 884.270 and 1030.348) were found only in BC-I. These findings could provide pivotal information on galactosylated N/O-linked glycans related to breast cancer, and promote the study of biomarkers for early-stage breast cancer based on precise alterations of galactosylated N/O-glycans in saliva.

2.
Breast Cancer ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-32030658

RESUMO

BACKGROUND: To date, anatomic tumor length is a key criterion for cancer staging and can be used to evaluate the effectiveness of therapies. This article describes growth pattern that can be used as a new characteristic to represent disease burden and tumor features and predict lymphatic metastasis. METHODS: Patients with breast cancer were included in this 10-year (1999-2008) hospital-based multicenter retrospective study. The pathologic length/height ratio was used to illustrate the correlation between tumor features, behaviors and treatments in breast malignancies. The most appropriate ratio was chosen based on the comprehensive evaluation of p value and changing trend of each characteristic. RESULTS: The sample consisted of 4211 women diagnosed with breast cancer. Among them, 2037 patients with complete pathologic length, width and height information were included in the final analysis. There were 2.34 ± 4.77 metastatic lymph nodes for spheroid tumors and 3.21 ± 5.82 for ellipsoid tumors when the cutoff point was 2. In addition, the proportion of ellipsoidal tumors gradually increased from 54.36 to 56.67% in the upper outer quadrant (UOQ) and from 6.7 to 9.03% in the central region with an increase in the cutoff point. The proportion of ER + PR + ellipsoid tumors significantly decreased from 50.1 to 45.35% and that of ER-PR ellipsoid tumors significantly increased from 32.73 to 36.24% with an increase in the cutoff point. Additionally, the best length/weight ratio to distinguish spheroid and ellipsoid tumors was 2. CONCLUSION: This study described for the first time how growth pattern is correlated with tumor malignancy and how it influences the selection of therapeutic strategies for patients.

3.
Environ Sci Technol ; 54(3): 1344-1352, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31766848

RESUMO

The Beijing government implemented a number of clean air action plans to improve air quality in the last 10 years, which contributed to changes in the concentration of fine particles and their compositions. However, quantifying the impacts of these interventions is challenging as meteorology masks the real changes in observed concentrations. Here, we applied a machine learning technique to decouple the effect of meteorology and evaluate the changes in the chemistry of nonrefractory PM1 (particulate matter less than 1 µm) in winter 2007, 2016, and 2017 as a result of the clean air actions. The observed mass concentrations of PM1 were 74.6, 90.2, and 36.1 µg m-3 in the three winters, while the deweathered concentrations were 74.2, 78.7, and 46.3 µg m-3, respectively. The deweathered concentrations of PM1, organics, sulfate, ammonium, chloride, SO2, NO2, and CO decreased by -38, -46, -59, -24, -51, -89, -16, and -52% in 2017 in comparison to 2007. On the contrary, the deweathered concentration of nitrates increased by 4%. Our results indicate that the clean air actions implemented in 2017 were highly effective in reducing ambient concentrations of SO2, CO, and PM1 organics, sulfate, ammonium, and chloride, but the control of nitrate and PM1 organics remains a major challenge.

4.
Int Immunopharmacol ; : 106025, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31810886

RESUMO

Plasma cell mastitis (PCM) is a special form of mastitis characterized by periductal inflammation and large-scale plasma cell infiltration. At present, the recurrence rate of PCM after excision is quite high, making PCM a major problem for mammary surgeons. However, no effective drug exists for the treatment of PCM. Numerous studies have demonstrated that Sinomenine hydrochloride (SH) has potent anti-inflammatory and immunoregulatory properties. However, the efficacy and the underlying mechanisms of SH in the treatment of PCM remain unclear. In the present study, we first investigated the therapeutic effects of SH in the PCM mouse model and clarified the possible mechanisms. We found that the levels of plasmocytes and lymphocytes infiltration were alleviated significantly in the 100 mg/kg SH group compared to the control group. In addition, few CD138+ plasma cells were found in the mammary glands of the 100 mg/kg SH group. The levels of Bcl-2 in the 100 mg/kg SH group were dramatically decreased compared with those in the saline group. Mechanistically, we demonstrated that SH inhibited the progression of PCM mainly through downregulating IL-6/JAK2/STAT3 levels. Collectively, our results suggested that SH could inhibit the progression of PCM by suppressing IL-6/JAK2/STAT3 cascades and ultimately achieve a therapeutic effect in PCM. This study provides theoretical support for the clinical application of SH in the treatment of PCM.

5.
BMC Cancer ; 19(1): 1091, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31718602

RESUMO

BACKGROUND: The features and survival of stage IV breast cancer patients with different metastatic sites are poorly understood. This study aims to examine the clinicopathological features and survival of stage IV breast cancer patients according to different metastatic sites. METHODS: Using the Surveillance, Epidemiology, and End Results database, we restricted our study population to stage IV breast cancer patients diagnosed between 2010 to 2015. The clinicopathological features were examined by chi-square tests. Breast cancer-specific survival (BCSS) and overall survival (OS) were compared among patients with different metastatic sites by the Kaplan-Meier method with log-rank test. Univariable and multivariable analyses were also performed using the Cox proportional hazard model to identify statistically significant prognostic factors. RESULTS: A total of 18,322 patients were identified for survival analysis. Bone-only metastasis accounted for 39.80% of patients, followed by multiple metastasis (33.07%), lung metastasis (10.94%), liver metastasis (7.34%), other metastasis (7.34%), and brain metastasis (1.51%). The Kaplan-Meier plots showed that patients with bone metastasis had the best survival, while patients with brain metastasis had the worst survival in both BCSS and OS (p < 0.001, for both). Multivariable analyses showed that age, race, marital status, grade, tumor subtype, tumor size, surgery of primary cancer, and a history of radiotherapy or chemotherapy were independent prognostic factors. CONCLUSION: Stage IV breast cancer patients have different clinicopathological characteristics and survival outcomes according to different metastatic sites. Patients with bone metastasis have the best prognosis, and brain metastasis is the most aggressive subgroup.

6.
Pathol Res Pract ; 215(11): 152644, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31551176

RESUMO

BACKGROUND: Chemotherapy is the predominant treatment option for patients with breast cancer. Selection of patients according to biomarker will improve chemotherapy efficacy. In the present study, we examined the relations of the expression of candidate genes and 21-recurrence score (RS) results to patients' demographic characteristics, histopathological factors, and outcomes. METHODS: A total of 146 patients were enrolled in this study. The patients underwent 21-gene RS testing. In addition, expressions of candidate genes, TYMS, RRM1, TUBB3, TOP2A, PTEN, were detected. Information was obtained on age, tumor size, TMN stage, tumor grade, and status of Ki-67, HER2, ER and PR. The treatment information on the type of endocrine therapy was also obtained. RESULTS: Results clearly showed that the 21-gene RS significantly correlated with the TNM stage of breast cancer (P = 0.047). The RS also correlated with the number of sentinel lymph node (P = 0.038). The pathological type of tumors was strongly associated with the expression of RRM1 (P < 0.015), and slightly correlated with TYMS (P = 0.095) and tumor size (P = 0.061). Further analysis showed that TYMS and RRM1 were two independent factors affecting the disease progression of patients. Besides, for HER-2 stain, staining of grade 2 or above significantly increased the risk of disease progression. CONCLUSION: Our studies showed that TNM stage and sentinel lymph node were important clinical parameters correlated with 21-gene RS results. Also, RRM1, TYMS and HER2 expressions were independent factors associated with disease progression in breast cancer patients. Future study is warranted to investigate the usefulness of these genes in treatment efficacy.

7.
Oncol Rep ; 42(5): 1699-1708, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31485677

RESUMO

Accumulated evidence indicates that aberrant microRNAs (miRNAs) expression plays an important role in the initiation and progression of various cancers, including breast cancer. Previous studies suggested that miR­139­3p might serve as a tumor suppressor and is downregulated in several cancer types. However, the expression patterns and exact role of miR­139­3p in breast cancer remain to be elucidated. In this study, we aimed to analyze the effect of miR­139­3p on the progression of breast cancer and the mechanism involved. Through bioinformatics analysis and in vitro experimental studies, we found that miR­139­3p was decreased in breast cancer tissues and cell lines, and decreased miR­139­3p is associated with a poor prognosis in breast cancer patients. Overexpression of miR­139­3p by transfection significantly inhibits cell proliferation, migration, and invasion of breast cancer cells. Bioinformatics analysis and luciferase reporter gene assay confirmed that RAB1A was a potential target of miR­139­3p. Furthermore, overexpression of RAB1A counteracted the suppressing effects of miR­139­3p on breast cancer cell migration, invasion and epithelial­to­mesenchymal transition (EMT). Taken together, these data suggest that miR­139­3p plays a tumor suppressive role in breast cancer by targeting RAB1A and may serve as a potential new biomarker for breast cancer prognosis.

8.
Medicine (Baltimore) ; 98(27): e16147, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31277115

RESUMO

BACKGROUND: As the most popular beverage in East Asia, green tea (GT) has various biological activities effects such as anti-mutation, anti-oxidation, and anti-tumor. In this study, we aimed to evaluate whether GT consumption could be an effective way to decrease the risk of breast cancer. METHODS: We had performed a systematic review and updated meta-analysis of published case-control studies to evaluate the association between GT intake and the risk of breast cancer. Searching strategies were performed by the following keywords "Breast cancer," "breast neoplasm," and "green tea," with derivations and different combinations. The following databases were searched: PubMed, Cochrane Library, EMBASE, Web of science, China National Knowledge Infrastructure, WanFang, and China Biology Medicine disc. Studies published in both English and Chinese were considered for inclusion. Risk of bias was assessed through the Newcastle-Ottawa Scale (NOS). All data were analyzed through using Review Manager 5.1 software. RESULTS: Fourteen studies fulfilled inclusion criteria for meta-analysis, yielding a total of 14,058 breast cancer patients and 15,043 control subjects. Individuals with the habit of drinking GT were found to have a negative association with the risk of future breast cancer (odds ratio 0.83; 95% confidence interval: 0.72-0.96) despite significant heterogeneity. In subgroup analyses, the negative correlation was still found in studies using registry-based controls, NOS grades ≥6 and the number of cases <500. CONCLUSIONS: GT consumption may have a decreased incidence of breast cancer despite significant heterogeneity. However, owing to the quality of available studies, more properly designed trials are warranted to clarify the association between GT consumption and breast cancer.


Assuntos
Neoplasias da Mama/prevenção & controle , Chá , Neoplasias da Mama/epidemiologia , Estudos de Casos e Controles , Feminino , Humanos
9.
Explore (NY) ; 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-31303328

RESUMO

BACKGROUND: Lymphoedema is a common complication of axillary dissection surgery, especially for breast cancer patients. Approximately 20% of breast cancer survivors develop breast cancer-related lymphoedema (BCRL). Acupuncture (AC) has become an alternative treatment for BCRL. In this study, we investigated whether AC was a good method for treating limb oedema in women after breast cancer surgery. METHODS: We performed a systematic review and meta-analysis of published randomized controlled trials (RCTs) to evaluate the effectiveness of AC in the prevention of BCRL. Searching strategies were performed with the following keywords: "Breast cancer," "Acupuncture," "neoplasm," and "lymphoedema," with derivations and different combinations of these keywords. The following databases were searched: PubMed, Cochrane Library, EMBASE, Web of Science, CNKI, WanFang, and CBM. Studies published in English and Chinese were considered for inclusion in this study. Study selection, risk of bias assessment and data extraction were independently conducted. Statistical analyses were conducted with RevMan software (version 5.3). RESULTS: Eight studies were identified by the search strategy, and 519 patients were included in this study. The effective rate was higher (odds ratios (OR): 4.23; 95% confidence interval (CI): 2.11 to 8.49; Z = 4.07, p < 0.0001) in the experimental group than that in the control group. There were no significant improvements in the front flexion (mean difference (MD): 0.19; 95% CI: -3.68 to 4.06; Z = 0.09, p = 0.92) or the back extension (MD: 0.42; 95% CI: -2.22 to 3.06; Z = 0.31, p = 0.75) movements of the shoulder between the experimental and control groups. CONCLUSIONS: AC may be an effective method for improving the condition of breast cancer-related lymphoedema. However, due to the high risk of bias and the low quality of the available studies, further high-quality RCTs are needed to confirm the efficacy of AC for breast cancer-related lymphoedema patients.

10.
Opt Lett ; 44(14): 3526-3529, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31305564

RESUMO

We propose and experimentally demonstrate a widely tunable coupled-cavity semiconductor laser based on a novel half-wave bow-tie coupler. The laser comprises two resonate cavities connected through the bow-tie coupler consisting of two back-to-back corner reflectors connected with a short waveguide, which produces a half-wave cross-coupling phase with an optimum coupling coefficient for achieving high mode selectivity. No complex grating or multiple epitaxial growth is needed. By tuning a single electrode at a fixed temperature, 29.6 nm wavelength tuning covering 38 channels with 100 GHz frequency spacing is achieved experimentally, with a side-mode suppression ratio of over 35 dB.

11.
Oncol Lett ; 17(6): 5469-5480, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31186766

RESUMO

The 21-gene recurrence score (RS) predicts the prognosis of patients with estrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative early-stage breast cancer and the effectiveness of adding adjuvant chemotherapy on the basis of endocrine therapy to avoid excessive chemotherapy. The present study aimed to analyze clinicopathological characteristics and chemotherapeutic efficacy-related target genes with the 21-gene RS in hormone receptor-positive early-stage breast cancer in China. The prognostic value of chemotherapeutic efficacy-related target genes was also examined. In addition, this study investigated the postoperative adjuvant therapeutic decision-oriented role of 21-gene RS in hormone receptor-positive and lymph node-negative early-stage breast cancer. In the present retrospective study, 110 ER+/HER2- early-stage breast cancer patients were tested with the 21-gene RS. The analyses of clinicopathological characteristics and chemotherapeutic efficacy-related target genes with the 21-gene RS were performed using the χ2 test, the Wilcoxon rank-sum test and binary logistic regression. Kaplan-Meier survival plots were drawn in www.kmplot.com. Furthermore, the McNemar χ2 test was used to compare the changes of treatment decisions before and after the 21-gene test. The median RS of 110 patients was 16 (range, 2-47), and patients were categorized as low (59.1%), intermediate (34.5%) or high risk (6.4%). The results revealed that higher body mass index, invasive ductal carcinoma type, higher histological grade, luminal B molecular type and higher thymidylate synthetase (TYMS) and DNA topoisomerase IIα (TOP2A) gene expression levels were more likely to have a higher RS. Kaplan-Meier plots suggested that expression of TYMS, tubulin ß3 class III (TUBB3) and TOP2A genes was significantly associated with relapse-free survival for ER+ breast cancer. Additionally, prior to 21-gene RS testing, 61 patients (55%) were recommended adjuvant chemotherapy and endocrine therapy; however, following 21-gene test, 32 patients (29%) were treated with only adjuvant endocrine therapy. TYMS, TUBB3 and TOP2A gene expression may have prognostic value for ER+ breast cancer. In addition, 21-gene RS testing may aid to avoid excessive postoperative adjuvant chemotherapy.

12.
Diagn Pathol ; 14(1): 35, 2019 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-31054583

RESUMO

BACKGROUND: The concordance rate of human epidermal growth factor receptor 2 (HER2) status between core needle biopsy (CNB) and subsequent excisional biopsies of the same tumor varies from 81 to 96%, which may cause inappropriate neoadjuvant therapy that impair the potential benefit from HER2 targeted therapy for patients. This study aimed to establish a nomogram to predict the HER2 status pre-operatively as an auxiliary diagnosis to CNB assessment. METHODS: Among 4211 breast cancer patients cataloged in the Nation-wide Multicenter 10-year Retrospective Clinical Epidemiological Study of Breast Cancer in China, 2291 patients with complete relevant information were included in this study, which were further randomized 3:1 and divided into a training set and a validation set. The nomogram was established based on independent predictors of HER2 positivity recognized by logistic regression analysis and further validated internally and externally. RESULTS: The multivariate logistic regression analysis showed that T-stage, N-stage, estrogen receptor (ER) status, progesterone receptor (PR) status were independent predictors for HER2 status. The nomogram was thereby constructed by those independent predictors as well as histology type. The areas under the receiver operating characteristic curve (AUC) of the training set and the validation set were 0.636 and 0.681, respectively. The calibration plots demonstrated good fitness of the nomogram for HER2 status prediction. With the optimal cutoff value, the nomogram yielded 80.0% sensitivity, 43.1% specificity in the training set and 81.1% sensitivity, 49.8% specificity in the validation set. CONCLUSIONS: The present nomogram can provide valuable information on HER2 status and combined with standard CNB assessment, clinicians could make more appropriate decision on neoadjuvant therapy of breast cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias da Mama/diagnóstico , Receptor ErbB-2/metabolismo , Biópsia com Agulha de Grande Calibre , Mama/patologia , Neoplasias da Mama/patologia , Neoplasias da Mama/terapia , China , Feminino , Humanos , Imuno-Histoquímica , Terapia Neoadjuvante , Estadiamento de Neoplasias , Nomogramas , Curva ROC , Estudos Retrospectivos , Risco , Sensibilidade e Especificidade
13.
Cancer Manag Res ; 11: 1503-1515, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30863160

RESUMO

Introduction: Aquaporins (AQPs), also called water channels, have been shown to have functions in the migration, invasion, and proliferation of human breast tumor cells. Most AQP mRNA expression levels were tested by cell lines, mouse models, and even human breast tissues. However, the mRNA expression of individual AQPs in different clinicopathologic characteristics and prognostic values according to different kinds of classifications of breast cancer patients remains unclear. Materials and methods: In the current study, we used the Oncomine database, Breast cancer Gene-Expression Miner v4.1, Kaplan-Meier Plotter, and cBioPortal to investigate the expression distribution and prognostic values of AQPs in breast cancer patients. Results: Our study revealed that the mRNA expression levels of AQP8, AQP9, and AQP10 were upregulated, while those of AQP3, AQP4, AQP5, and AQP7 were downregulated in breast cancer. The clinical database showed that lower mRNA levels of AQP1 were associated with a high Scarff-Bloom-Richardson grade, but AQP9 showed the opposite trend. Further survival analyses indicated that high mRNA expression levels of AQP0, AQP1, AQP2, AQP4, AQP6, AQP8, AQP10, and AQP11 were significantly associated with better relapse-free survival (RFS). Conversely, AQP3 and AQP9 were associated with worse RFS in breast cancer patients, suggesting that these two genes might be potential targets in future chemotherapy. Discussion: These significant AQP members might be further explored as new biomarkers for breast cancer prognosis, but this needs further study.

14.
Anal Bioanal Chem ; 411(21): 5373-5382, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30820628

RESUMO

Circulating tumor cells (CTCs) are extraordinarily rare in blood samples and represent a real-time "liquid biopsy" of tumors. Although genetic and transcriptional sequencing of single CTCs has been reported, these methods fail to provide phenotypic and functional information of CTCs such as protein levels of surface proteins. Studies of single-cell proteomic assays of CTCs have been rare because of a lack of single-cell proteomic methods to handle and analyze rare cells in a high background of non-target cells with high sensitivity, throughput, and multiplexing capacity. Here, we develop a microchip-assisted single-cell proteomic method for profiling surface proteins of CTCs based on antibody and cellular DNA barcoding strategy. We combine DNA-encoded antibody tags and cell indexes to profile 15 proteins in ~ 100 single rare cells simultaneously, and use high-throughput sequencing as the readout to generate surface protein profiles of CTCs according to their cell indexes and antibody-derived protein barcodes. A 6400-well microchip and the automated puncher are used to rapidly retrieve single CTCs from enriched CTC population with minimal cell loss (~ 10%). This technological platform integrates reliable isolation and proteomic analysis of single CTCs and can be extendable to ~ 100 proteins in hundreds of rare cells with single-cell precision.


Assuntos
Anticorpos/imunologia , Código de Barras de DNA Taxonômico , Proteínas de Membrana/metabolismo , Proteínas de Neoplasias/metabolismo , Células Neoplásicas Circulantes/metabolismo , Análise de Célula Única/métodos , Antígenos de Neoplasias/imunologia , Linhagem Celular Tumoral , Ensaios de Triagem em Larga Escala , Humanos , Proteínas de Membrana/genética , Proteínas de Membrana/imunologia , Proteínas de Neoplasias/genética , Células Neoplásicas Circulantes/imunologia , Reprodutibilidade dos Testes
15.
J Healthc Eng ; 2019: 6035920, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30886686

RESUMO

Background: Few studies have evaluated depression in female caregivers of patients with silicosis. Thus, the aim of this study was to estimate the prevalence of depression in such caregivers and to clarify the factors associated with symptoms of depression. Methods: Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression Scale (CES-D). Results: A total of 561 participants met the inclusion criteria and were enrolled in the study. The mean CES-D score was 16.68, with a standard deviation (SD) of 8.57; the sex-classified analysis indicated that the mean CES-D score of female caregivers was 17.79 (SD: 10.17), while the mean score of male caregivers was 14.98 (SD: 8.36) (p < 0.05). 68.6% caregivers who were beyond the cutoff score (16) with the following factors were more likely to report depression-related symptoms: unemployed status (OR = 1.752, 95% CI: 1.35-2.01, p=0.032) and caregiver for more than 48 months (OR = 1.26, 95% CI: 1.61-2.43, p=0.027). Conclusions: Collectively, there is statistical difference between female caregivers of patients with silicosis and male ones. More effort is needed to meet the psychosocial needs of these caregivers.

16.
Mikrochim Acta ; 186(3): 160, 2019 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-30721353

RESUMO

Strongly fluorescent polymers (FPs) were prepared from citric acid and ethylenediamine via a hydrothermal approach. The FPs display low toxicity, water solubility, a quantum yield of 91%, good photostability and stability in the physiological pH range. Ferric ions are found to quench the fluorescence which is best measured at excitation/emission wavelengths of 350/440 nm. Because ferric ions (Fe3+) can quench the fluorescence of FPs, a fluorometric method was developed for fast detection of Fe3+ and within 1 min. FPs can also be used indirectly for the detection of hydrogen peroxide because of its fast Fenton reaction with Fe2+ to generate of Fe3+. The detection limits are 8 µM for Fe(III) and 0.6 µM for H2O2. On the basis of the glucose oxidase catalyzed of glucose and the Fenton reaction, the FPs can also be used to quantify glucose with a linear response in the 0.5-10 µM concentration range. Graphical abstract A new type of polymer with high fluorescence quantum yield was prepared. It is shown to enable the fast detection of ferric ions, hydrogen peroxide and glucose based on quenching and on the glucose oxidase and Fenton reactions.


Assuntos
Fluorometria/métodos , Glucose/análise , Peróxido de Hidrogênio/análise , Compostos Férricos , Fluorescência , Glucose/metabolismo , Glucose Oxidase/metabolismo , Limite de Detecção , Polímeros/síntese química
17.
Opt Lett ; 44(2): 211-214, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30644863

RESUMO

We designed and fabricated a silica-based 16×16 cyclic arrayed waveguide grating router (AWGR) with improved channel loss uniformity using a simple mode-field converter composed of a slab coupling region and auxiliary waveguides placed at the interface between the arrayed waveguides and the output star coupler of the AWGR. The mode-field converter transforms the fundamental Gaussian-shaped mode in the arrayed waveguides to a complex mode field which produces a flat-top far field at the image plane of the output star coupler. It does not change the overall construction of the AWGR and does not increase the device size. The experimental results show that loss non-uniformity for a 16×16 AWGR with 1.6 nm wavelength channel spacing is reduced from 3 to 0.5 dB after adapting the mode-field converter, and the crosstalk is improved by about 2 dB.

18.
Opt Lett ; 44(2): 299-302, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30644885

RESUMO

We demonstrate a temperature-insensitive waveguide sensor based on a silicon-on-insulator platform. The sensor consists of a ring resonator and a Mach-Zehnder interferometer (MZI). A free spectral range of the sensing ring is designed to be slightly different from that of the MZI; hence, the Vernier effect can be employed to improve sensitivity. By optimizing structural parameters of the MZI, the envelope peak position of a cascaded transmission spectrum can be immune to the temperature variation, and only dependent on analyte change in the sensing area. The experimental results show that bulk refractive index (RI) sensitivity of the proposed sensor is 3552 nm/RI unit, while its temperature sensitivity is less than 4 pm/K, which is two orders of magnitude smaller than the conventional cascaded sensor structure without temperature compensation. The proposed temperature-insensitive waveguide sensor does not need polymer cladding or extra thermal stabilization, making it more robust in practical applications.

19.
Int J Cancer ; 144(2): 281-289, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-29752822

RESUMO

Multigene panel testing of breast cancer predisposition genes have been extensively conducted in Europe and America, which is relatively rare in Asia however. In this study, we assessed the frequency of germline mutations in 40 cancer predisposition genes, including BRCA1 and BRCA2, among a large cohort of Chinese patients with high hereditary risk of BC. From 2015 to 2016, consecutive BC patients from 26 centers of China with high hereditary risk were recruited (n = 937). Clinical information was collected and next-generation sequencing (NGS) was performed using blood samples of participants to identify germline mutations. In total, we acquired 223 patients with putative germline mutations, including 159 in BRCA1/2, 61 in 15 other BC susceptibility genes and 3 in both BRCA1/2 and non-BRCA1/2 gene. Major mutant non-BRCA1/2 genes were TP53 (n = 18), PALB2 (n = 11), CHEK2 (n = 6), ATM (n = 6) and BARD1 (n = 5). No factors predicted pathologic mutations in non-BRCA1/2 genes when treated as a whole. TP53 mutations were associated with HER-2 positive BC and younger age at diagnosis; and CHEK2 and PALB2 mutations were enriched in patients with luminal BC. Among high hereditary risk Chinese BC patients, 23.8% contained germline mutations, including 6.8% in non-BRCA1/2 genes. TP53 and PALB2 had a relatively high mutation rate (1.9 and 1.2%). Although no factors predicted for detrimental mutations in non-BRCA1/2 genes, some clinical features were associated with mutations of several particular genes.


Assuntos
Neoplasias da Mama/genética , Predisposição Genética para Doença/genética , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Mutação em Linhagem Germinativa , Humanos , Pessoa de Meia-Idade
20.
Int J Biochem Cell Biol ; 107: 6-13, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30447427

RESUMO

BACKGROUND: The expression of p38 MAPK is high in breast cancer while its subunit p38γ had been rarely reported. We aimed to explain the effect of p38γ in breast cancer from the perspective of metabolomics. METHODS: In this study, we detected the expression of p38γ in 28 breast carcinoma and para-tumor samples. Following MDA-MB-231 cell transfection with p38γ siRNAs and pc-DNA-3.1, cell viability, apoptosis, metastasis were determined through CCK-8, the cytometry analysis, transwell assay and wound healing assay. Finally, gas chromatograph-mass spectrometer (GC-MS) was used for analysis the differential metabolites. RESULTS: The expression of p38γ was significantly up-regulated in breast cancer tissues. The transfection of si-p38γs could inhibit MDA-MB-231 cell propagation, metastasis, and induced cell apoptosis while overexpressed p38γ could promote the cell propagation, metastasis, and inhibit cell apoptosis. A total of 238 metabolites were identified and 72 of them differentially expressed in three groups (all P < 0.05, FDR < 0.05). Then the metabolites were enriched in the metabolism pathway, 85 pathways were included and 27 were significant (all P < 0.05, FDR < 0.05). CONCLUSIONS: p38γ was up-regulated in breast cancer, which exerts a great influence on the cell growth, cell mobility, invasiveness, and apoptosis of MDA-MB-231 cells and also affected the metabolism.


Assuntos
Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Metabolômica , Proteína Quinase 12 Ativada por Mitógeno/metabolismo , Apoptose , Neoplasias da Mama/genética , Linhagem Celular Tumoral , Movimento Celular , Sobrevivência Celular , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Pessoa de Meia-Idade , Proteína Quinase 12 Ativada por Mitógeno/genética , Invasividade Neoplásica
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