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1.
Neural Regen Res ; 17(8): 1776-1784, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35017438

RESUMO

As a highly evolutionary conserved long non-coding RNA, metastasis associated lung adenocarcinoma transcript 1 (MALAT1) was first demonstrated to be related to lung tumor metastasis by promoting angiogenesis. To investigate the role of MALAT1 in traumatic brain injury, we established mouse models of controlled cortical impact and cell models of oxygen-glucose deprivation to mimic traumatic brain injury in vitro and in vivo. The results revealed that MALAT1 silencing in vitro inhibited endothelial cell viability and tube formation but increased migration. In MALAT1-deficient mice, endothelial cell proliferation in the injured cortex, functional vessel density and cerebral blood flow were reduced. Bioinformatic analyses and RNA pull-down assays validated enhancer of zeste homolog 2 (EZH2) as a downstream factor of MALAT1 in endothelial cells. Jagged-1, the Notch homolog 1 (NOTCH1) agonist, reversed the MALAT1 deficiency-mediated impairment of angiogenesis. Taken together, our results suggest that MALAT1 controls the key processes of angiogenesis following traumatic brain injury in an EZH2/NOTCH1-dependent manner.

2.
Mol Med Rep ; 25(3)2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35039872

RESUMO

Long non­coding RNAs (lncRNAs) feature prominently in pancreatic carcinoma progression. The present study aimed to clarify the biological functions, clinical significance and underlying mechanism of lncRNA CTBP1 antisense RNA 2 (CTBP1­AS2) in pancreatic carcinoma. Reverse transcription­quantitative PCR was performed to assess the expression levels of CTBP1­AS2, microRNA (miR)­141­3p and ubiquitin­specific protease 22 (USP22) mRNA in pancreatic carcinoma tissues and cell lines. Western blotting was used to examine USP22 protein expression in pancreatic carcinoma cell lines. Loss­of­function experiments were used to analyze the regulatory effects of CTBP1­AS2 on proliferation, apoptosis, migration and invasion of pancreatic carcinoma cells. Dual­luciferase reporter assay was used to examine the binding relationship between CTBP1­AS2 and miR­141­3p, as well as between miR­141­3p and USP22. It was demonstrated that CTBP1­AS2 expression was markedly increased in pancreatic carcinoma tissues and cell lines. High CTBP1­AS2 expression was associated with advanced clinical stage and lymph node metastasis of patients. Functional experiments confirmed that knocking down CTBP1­AS2 significantly inhibited pancreatic carcinoma cell proliferation, migration and invasion, and promoted cell apoptosis. In terms of mechanism, it was found that CTBP1­AS2 adsorbed miR­141­3p as a molecular sponge to upregulate the expression level of USP22. In conclusion, lncRNA CTBP1­AS2 may be involved in pancreatic carcinoma progression by regulating miR­141­3p and USP22 expressions; in addition, CTBP1­AS2 may be a diagnostic biomarker and treatment target for pancreatic carcinoma.

3.
Artigo em Inglês | MEDLINE | ID: mdl-35042337

RESUMO

Carbonaceous materials featuring both ordered graphitic structure and disordered defects hold great promise for high-performance K-ion batteries (KIBs) due to the concurrent advantages of high electronic conductivity, fast and reversible K+ intercalation/deintercalation, and abundant active K+ storage sites. However, it has been a lingering problem and remains a big challenge because graphitization and defects are intrinsic trade-off properties of carbonaceous materials. Herein, for the first time, we propose a cobalt-catalyzed carbonization strategy to fabricate porous carbon nanofibers that incorporate disordered defects in graphitic domain layers (PCNFs-DG) for fast and durable K+ storage. The Co catalyst not only ensures the formation of highly graphitized carbon shells around the Co particles but also introduces nanopores and doping defects in the following catalyst removal process. This idea of architecting defected-ordered graphitic carbon engineering guarantees fast reaction kinetics as well as structural stability with negligible interlayer expansion/contraction owing to the uncompromised electronic conductivity, expanded interlayer spacing, and regulated K+ storage mechanism. These appealing features translate to a high reversible capacity of 318.5 mAh g-1 at 100 mA g-1 and ultrahigh stability with almost 100% capacity retention over 2000 cycles in KIBs. This work puts in perspective that defected and ordered carbonaceous materials could be simultaneously achieved, advancing their performance for next-generation energy storage systems.

4.
PLoS One ; 17(1): e0261216, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35030181

RESUMO

BACKGROUND: The global epidemic of novel coronavirus pneumonia (COVID-19) has resulted in substantial healthcare resource consumption. Since patients' hospital length of stay (LoS) is at stake in the process, an investigation of COVID-19 patients' LoS and its risk factors becomes urgent for a better understanding of regional capabilities to cope with COVID-19 outbreaks. METHODS: First, we obtained retrospective data of confirmed COVID-19 patients in Sichuan province via National Notifiable Diseases Reporting System (NNDRS) and field surveys, including their demographic, epidemiological, clinical characteristics and LoS. Then we estimated the relationship between LoS and the possibly determinant factors, including demographic characteristics of confirmed patients, individual treatment behavior, local medical resources and hospital grade. The Kaplan-Meier method and the Cox Proportional Hazards Model were applied for single factor and multi-factor survival analysis. RESULTS: From January 16, 2020 to March 4, 2020, 538 human cases of COVID-19 infection were laboratory-confirmed, and were hospitalized for treatment, including 271 (50%) patients aged ≥ 45, 285 (53%) males, and 450 patients (84%) with mild symptoms. The median LoS was 19 (interquartile range (IQR): 14-23, range: 3-41) days. Univariate analysis showed that age and clinical grade were strongly related to LoS (P<0.01). Adjusted multivariate analysis showed that the longer LoS was associated with those aged ≥ 45 (Hazard ratio (HR): 0.74, 95% confidence interval (CI): 0.60-0.91), admission to provincial hospital (HR: 0.73, 95% CI: 0.54-0.99), and severe illness (HR: 0.66, 95% CI: 0.48-0.90). By contrast, the shorter LoS was linked with residential areas with more than 5.5 healthcare workers per 1,000 population (HR: 1.32, 95% CI: 1.05-1.65). Neither gender factor nor time interval from illness onset to diagnosis showed significant impact on LoS. CONCLUSIONS: Understanding COVID-19 patients' hospital LoS and its risk factors is critical for governments' efficient allocation of resources in respective regions. In areas with older and more vulnerable population and in want of primary medical resources, early reserving and strengthening of the construction of multi-level medical institutions are strongly suggested to cope with COVID-19 outbreaks.

5.
Biodegradation ; 2022 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-35025000

RESUMO

Petroleum-based plastics (PBP) with different properties have been developed to suit various needs of modern lives. Nevertheless, these well-developed properties also present the double-edged sword effect that significantly threatens the sustainability of the environment. This work focuses on the impact of microbial cultivating conditions (the elementary compositions and temperature) to provide insightful information for the process optimization of microbial degradation. The major elementary compositions in cultivation media and temperature from the literature were radically reviewed and assessed using the constructed supervised machine learning algorithm. Fifty-two literatures were collected as a training dataset to investigate the impact of major chemical elements and cultivation temperature upon PBP biodegradation. Among six singular parameters (NH4+, K+, PO43-, Mg2+, Ca2+, and temperature) and thirty corresponding binary parameters, four singular (NH4+, K+, PO43-, and Mg2+) and six binary parameters (NH4+/K+, NH4+/PO43-, NH4+/Ca2+, K+/PO43-, PO43-/Mg2+, Mg2+/Temp) were identified as statistically significant towards microbial degradation through analysis of variance (ANOVA). The binary effect (PO43-/Mg2+) is found to be the most statistically significant towards the microbial degradation of PBP. The concentration range, which locates at 0.1-0.6 g/L for Mg2+ and 0-2.8 g/L for PO43-, was identified to contribute to the maximum PBP biodegradation. Among all the investigated elements, Mg2+ is the only element that is statistically and significantly associated with the variations of cultivation temperature. The optimal preparation conditions within ± 20% uncertainties based upon the range of collected literature reports are recommended. Five representative cultivation elementary compositions (NH4+, K+, PO43-, Mg2+, and Ca2+) and temperature were reviewed from fifty two different literature reports to investigate their impacts on the microbial degradation of PBP using supervised machine learning algorithm. The optimal cultivation conditions based upon collected literature reports to achieve biodegradation over 80% were identified.

6.
Tissue Cell ; 74: 101716, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34979377

RESUMO

Apart from the treatment potential of micro-fragmented adipose tissue (MF) in joint diseases, what's less clear is the mechanism of MF on Osteoarthritis (OA). Synoviocytes isolated from synovium tissues of 11 knee joint OA patients were identified and co-cultured with MF collected by Lipogems®. Cytokines and mRNA levels in synoviocytes were detected by enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell viability, apoptosis and apoptosis-related protein expression of Tumor Necrosis Factor-α (TNF-α)-activated synoviocytes were detected by cell counting kit-8, flow cytometry and western blot, respectively. The rescue experiments were conducted to verify the causal relationship of MF and miR-92a-3p. The relationship between miR-92a-3p and KLHL29 was verified by bioinformatics analysis, qRT-PCR, dual-luciferase reporter assay and western blot. OA synoviocytes were composed of synovial fibroblasts and synovial macrophages. After co-cultivation of synoviocytes and TNF-α, the levels of Interleukin (IL)-8 and hyaluronic acid (HA) appeared a few changes, and those of chemotactic cytokine ligand (CCL) 2, CCL3, CCL5 and matrix metalloproteinases (MMP)-9 were downregulated, while the levels of Tissue Inhibitor of Metalloproteinases (TIMP)-1, IL-10 and Prostaglandin E2 (PGE2) were up-regulated. Co-culture of MF and activated synoviocytes reversed the above-mentioned effects regulated by TNF-α and reduced the mRNA levels of inflammatory factors. However, miR-92a-3p inhibitor overturned the reversal. KLHL29 was the target gene of miR-92a-3p and its expression was suppressed in activated synoviocytes co-cultured with MF, which was reversed by down-regulated miR-92a-3p. Collectively, MF regulated the biological functions of OA synoviocytes by upregulating miR-92a-3p expression.

7.
Parasit Vectors ; 15(1): 22, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35012632

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) are important regulators of various biological and pathological processes, in particular the inflammatory response by modulating the transcriptional control of inflammatory genes. However, the role of lncRNAs in regulating the immune and inflammatory responses during infection with the protozoan parasite Toxoplasma gondii remains largely unknown. METHODS: We performed a longitudinal RNA sequencing analysis of human foreskin fibroblast (HFF) cells infected by T. gondii to identify differentially expressed long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs), and dysregulated pathways over the course of T. gondii lytic cycle. The transcriptome data were validated by qRT-PCR. RESULTS: RNA sequencing revealed significant transcriptional changes in the infected HFFs. A total of 697, 1234, 1499, 873, 1466, 561, 676 and 716 differentially expressed lncRNAs (DElncRNAs), and 636, 1266, 1843, 2303, 3022, 1757, 3088 and 2531 differentially expressed mRNAs (DEmRNAs) were identified at 1.5, 3, 6, 9, 12, 24, 36 and 48 h post-infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DElncRNAs and DEmRNAs revealed that T. gondii infection altered the expression of genes involved in the regulation of host immune response (e.g., cytokine-cytokine receptor interaction), receptor signaling (e.g., NOD-like receptor signaling pathway), disease (e.g., Alzheimer's disease), and metabolism (e.g., fatty acid degradation). CONCLUSIONS: These results provide novel information for further research on the role of lncRNAs in immune regulation of T. gondii infection.

8.
Anim Nutr ; 8(1): 256-264, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34988307

RESUMO

This study aimed to investigate effects of dietary lycopene supplementation on meat quality, antioxidant ability and muscle fiber type transformation in finishing pigs. In a 70-day experiment, 18 Duroc × Landrace × Yorkshire barrows were randomly allocated to 3 dietary treatments including a basal diet supplemented with 0, 100 and 200 mg/kg lycopene, respectively. Each dietary treatment had 6 replicates with one pig each. Results showed that dietary 200 mg/kg lycopene supplementation increased muscle redness a∗ value, intramuscular fat and crude protein contents, and decreased muscle lightness L∗ and yellowness b∗ values (P < 0.05), suggesting that addition of 200 mg/kg lycopene to the diet of finishing pigs improved color, nutritional value and juiciness of pork after slaughter. Results also showed that dietary lycopene supplementation enhanced antioxidant capacity of finishing pigs (P < 0.05). Moreover, dietary supplementation of 200 mg/kg lycopene significantly increased slow myosin heavy chain (MyHC) protein level and slow-twitch fiber percentage, and decreased fast MyHC protein level and fast-twitch fiber percentage (P < 0.05), suggesting that the addition of 200 mg/kg lycopene to the diet of finishing pigs promoted muscle fiber type conversion from fast-twitch to slow-twitch. Together, we provide the first evidence that dietary 200 mg/kg lycopene supplementation improves meat quality, enhances antioxidant capacity and promotes muscle fiber type transformation from fast-twitch to slow-twitch in finishing pigs.

9.
Adv Mater ; : e2108313, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34989444

RESUMO

Copper chalcogenides represent a class of materials with unique crystal structures, high electrical conductivity and earth abundance, and has been recognized as promising candidates for next-generation green electronics. However, their two-dimensional (2D) structures and the corresponding electronic properties have rarely been touched. Herein, we have successfully synthesized a series of ultrathin copper chalcogenide nanosheets with thicknesses down to two unit cells, including layered Cu2 Te, as well as nonlayered CuSe and Cu9 S5 , via van der Waals epitaxy, and investigated their nonvolatile memristive behavior for the first time. Benefiting from the highly active Cu ions with low migration barriers, the memristors based on ultrathin 2D copper chalcogenide crystals exhibit relatively small switching voltage (∼0.4 V), fast switching speed, high switching uniformity and wide operating temperature range (from 80 K to 420 K), as well as stable retention and good cyclic endurance. These results demonstrate their tangible applications in future low-power, cryogenic and high temperature harsh electronics. This article is protected by copyright. All rights reserved.

10.
ACS Appl Mater Interfaces ; 14(1): 1070-1076, 2022 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-34955027

RESUMO

With 2-COOH and 4-SH donors all packed onto the benzene ring, tetrasulfanyl terephthalic acid (TST) is a simple yet fully equipped ligand to move the field of metal-coordination materials─it is now accomplished. The hard-soft carboxyl-thiol synergy is leveraged here in selectively bonding the carboxyl units to Zr(IV) ions to form the same cubic net of UiO-66 (this being based on the terephthalic linker)─with the free-standing dithiolene units equipping the grid of ZrTST. The 3D network of ZrTST averages about 7.6 connections [as in Zr6O4(OH)4(C8H4O4S4)3.8], with the other 4.4 sealed by acetate ions. The ZrTST solid is stable in boiling water (it is formed in water/acetic acid/ethane dithiol) and remains ordered even above 300 °C. The thiol-enabled ZrTST (powder) takes up mercury from water with a high distribution coefficient Kd (e.g., 1.2 × 106 mL·g-1); it also shows proton conductivity (1.9 × 10-3 S·cm-1 at 90 °C and 90% relative humidity), which, most notably, increases to a highest value of 3.7 × 10-1 S·cm-1 after oxidizing the -SH into the -SO3H groups.

11.
J Asian Nat Prod Res ; 24(1): 76-87, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34937462

RESUMO

Renal fibrosis is a critical pathological process lead to a progressive loss of renal function. Jolkinolide B (JB) is a natural compound with anti-inflammatory activity from Euphorbia fischeriana Steud. The study evaluated the effect of JB on renal fibrosis in mice with unilateral ureteral obstruction (UUO). The results showed that JB could decrease renal fibrotic area, reduce phosphorylation of NF-κB p65 and the release of TNF-α, IL-6 and IL-1ß, restore the expression of vementin, α-SMA and E-cadherin, as well as TGF-ß1 and p-smad2/3. In conclusion, JB might reduce renal fibrosis by inhibiting inflammation induced by NF-κB pathway and EMT mediated by TGF-ß1/Smad pathway.


Assuntos
Obstrução Ureteral , Animais , Anti-Inflamatórios/farmacologia , Diterpenos , Transição Epitelial-Mesenquimal , Fibrose , Rim/patologia , Camundongos , Estrutura Molecular , Transdução de Sinais , Fator de Crescimento Transformador beta1/farmacologia , Fator de Crescimento Transformador beta1/uso terapêutico , Obstrução Ureteral/tratamento farmacológico , Obstrução Ureteral/patologia
12.
Food Chem ; 366: 130618, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34330028

RESUMO

The objective of this study was to compare the effects of the micronization (MT), steam explosion (SE), and gamma irradiation (GI) treatment on the chemical composition, structure, physicochemical properties, and in vitro digestibility of dietary fiber from soybean hulls. GI (1200 kGy) treatment exerted the optimum effects on improving soluble dietary fiber content, in vitro gross energy digestibility (IVGED), and reducing sugar yield (RS) in the three modification methods, increased by 342.88%, 55.24%, and 117.02%, respectively. Compared with GI treatment, MT-GI combined treatment could further enhance the degradation effect of irradiation and improve the physicochemical properties (p<0.05) in soybean fibers. From the results of correlation analysis, RS was a significant positive correlation (p<0.05) with IVGED, and RS = -112.24 + 4.90 × IVGED (r2 = 0.82, p<0.01). In summary, MT-GI combined treatment could be considered the ideal modification method to improve the quality of soybean fiber.


Assuntos
Soja , Vapor , Ração Animal/análise , Fibras na Dieta/análise , Digestão
13.
Food Chem ; 367: 130781, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34391997

RESUMO

The aim of this study was to investigate effects of dietary grape seed proanthocyanidin extract (GSPE) supplementation on meat quality, muscle fiber characteristics and antioxidant capacity of finishing pigs. The data showed GSPE increased pH24 h, redness, crude protein content and decreased shear force, drip loss48 h, lactate content and glycolytic potential in longissimus dorsi (LD) muscle, accompanied by increased contents of total polyunsaturated fatty acid (PUFA), n-3 PUFA, and the ratio of PUFA to saturated fatty acid. GSPE promoted MyHC I mRNA and slow MyHC protein expression, and increased slow-twitch fiber percentage. The activities of total antioxidant capacity, total superoxide dismutase, catalase and glutathione peroxidase in LD muscle were increased by GSPE while malondialdehyde content was decreased. Together, this study demonstrated that dietary GSPE supplementation can effectively improve the color, water-holding capacity, tenderness and nutritional value of pork, and increase slow-twitch fiber percentage and antioxidant capacity of finishing pigs.


Assuntos
Antioxidantes , Extrato de Sementes de Uva , Ração Animal/análise , Suplementos Nutricionais , Carne/análise , Fibras Musculares Esqueléticas , Proantocianidinas , Suínos
14.
Sci Total Environ ; 805: 150248, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34536865

RESUMO

Since the outbreak of COVID-19 pandemic, the lockdown policy across the globe has brought improved air quality while fighting against the coronavirus. After the closure, urban air quality was subject to emission reduction of air pollutants and rebounded to the previous level after the potency period of recession. Different response patterns exhibit divergent sensitivities of urban resilience in regard to air pollution. In this paper, we investigate the post-lockdown AQI values of 314 major cities in China to analyse their differential effects on the influence factors of urban resilience. The major findings of this paper include: 1) Cities exhibit considerable range of resilience with their AQI values which are dropped by 21.1% per day, took 3.97 days on average to reach the significantly decreased trough point, and reduced by 49.3% after the lockdown initiatives. 2) Mega cities and cities that locate as the focal points of transportation for nearby provinces, together with those with high AQI values, were more struggling to maintain a good air quality with high rebounds. 3) Urban resilience shows divergent spatial sensitivities to air pollution controls. Failing to consider multi-dimensional factors besides from geomorphological and economical activities could lead to uneven results of environmental policies. The results unveil key drivers of urban air pollution mitigation, and provide valuable insights for prediction of air quality in response to anthropogenic interference events under different macro-economic contexts. Research findings in this paper can be adopted for prevention and management of public health risks from the perspective of urban resilience and environmental management in face of disruptive outbreak events in future.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , COVID-19 , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China/epidemiologia , Cidades , Controle de Doenças Transmissíveis , Monitoramento Ambiental , Humanos , Pandemias , Material Particulado/análise , SARS-CoV-2
15.
Food Chem ; 371: 131156, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34583183

RESUMO

The spoiled dry-cured ham commonly shows unpleasant taste and odour. To deepen the understanding in the formation mechanism of unpleasant taste in spoiled ham, sensory attributes, texture parameters, protein degradation, metabolites were investigated between normal and spoiled hams; the relationship between the sensory quality and metabolites of dry-cured ham was further evaluated by partial least square discriminant analysis (PLS-DA). The scores of richness and overall acceptance were significantly lower in spoiled ham, and more than 12.5-fold values in adhesiveness were found in spoiled ham than normal ham. Myofibrillar proteins including actin, troponin-T and myosin light chain showed excessive degradation in spoiled ham. Forty-two kinds of metabolites mainly derived from protein degradation were identified by LC-MS/MS, and amino acid derivatives and oligopeptides were the key components to distinguish spoiled and normal hams demonstrated by PLS-DA. Purine metabolism, pyrimidine metabolism and protein degradation were the main metabolism pathways in spoiled ham.


Assuntos
Produtos da Carne , Carne de Porco , Cromatografia Líquida , Produtos da Carne/análise , Metabolômica , Espectrometria de Massas em Tandem
16.
Biosens Bioelectron ; 197: 113771, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34775255

RESUMO

Due to the similar clinical symptoms of influenza (Flu) and coronavirus disease 2019 (COVID-19), there is a looming infection threat of concurrent Flu viruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this work, we introduce a customized isothermal amplification integrated lateral flow strip (LFS) that is capable performing duplex reverse transcription-recombinase polymerase amplification (RT-RPA) and colorimetric LFS in a sequential manner. With customized amplification primer sets targeted to SARS-CoV-2 (opening reading frame 1a/b and nucleoprotein genes) and Flu viruses (Flu A and Flu B), the platform allows the rapid and simultaneous visual screening of SARS-CoV-2 and Flu viruses (Flu A and Flu B) without cross reactivity, false positives, and false negatives. Moreover, it maximally eases the detection, reduces the detection time (1 h), and improves the assay performance to detect as low as 10 copies of the viral RNA. Its clinical application is powerfully demonstrated with 100% accuracy for evaluating 15 SARS-CoV-2-positive clinical samples, 10 Flu viruses-positive clinical samples, and 5 negative clinical samples, which were pre-confirmed by standard qRT-PCR. We envision this portable device can meet the increasing need of online monitoring the serious infectious diseases that substantially affects health care systems worldwide.


Assuntos
Técnicas Biossensoriais , COVID-19 , Influenza Humana , Humanos , Influenza Humana/diagnóstico , Técnicas de Amplificação de Ácido Nucleico , SARS-CoV-2 , Sensibilidade e Especificidade
17.
Phytomedicine ; 94: 153832, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34781230

RESUMO

BACKGROUND: The combination of antiarrhythmic drugs with traditional Chinese formulas are used treatments for the management of paroxysmal atrial fibrillation (PAF). However, the most effective treatment for PAF has yet to be been determined. A Bayesian network meta-analysis study was thus performed for comparing the relative efficacy and tolerability of different treatment alternatives. METHODS: A comprehensive literature review of randomized controlled trials (RCTs) is performed from eight database. Maintenance rate of sinus rhythm (MRSR), p-wave dispersion (Pd), left atrium diameter (LAD), left ventricular ejection fraction (LVEF), and adverse events (AEs) were used as outcomes. We also estimated treatment rank based on the surface under the cumulative ranking curve (SUCRA). This study was performed using a Bayesian network meta-analysis with a random-effects model. FINDINGS: After screening, 59 RCTs involving 5,543 patients and 16 treatments were included. The results showed that Shensong-Yangxin capsule (SSYX) plus amiodarone (81%) was the most effective treatment for MRSR according to the value of SUCRA, followed by Wenxin-Keli granules (WXKL) plus amiodarone (73%). Meanwhile, SSYX plus amiodarone (7%) was most likely to reduce Pd, followed by SSYX plus metoprolol (23%), WXKL plus amiodarone (26%), WXKL plus bisoprolol (27%). Furthermore, SSYX plus amiodarone (4%) was more effective in improving LAD. WXKL plus amiodarone was preferred because it had the lowest toxicity. For benefit-risk ratio, amiodarone combined with WXKL or SSYX appeared to be the best option. CONCLUSION: Antiarrhythmic agents combined with traditional Chinese formulas had higher efficacy and lower toxicity than other treatment alternatives. This study might provide reference to help find the better treatment options for PAF.


Assuntos
Antiarrítmicos , Fibrilação Atrial , Antiarrítmicos/uso terapêutico , Fibrilação Atrial/tratamento farmacológico , China , Humanos , Metanálise em Rede
18.
J Ethnopharmacol ; 284: 114748, 2022 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-34662666

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The dried roots and rhizomes of Cynanchum atratum Bunge is named 'Baiwei' according to traditional Chinese medicine theory. It is also named Cynanchi atrati Radix in Chinese Pharmacopoeia. Cynanchi atrati Radix is famous for its medicinal value of clearing away heat, relieving drenching, detoxifying and treating abscesses. It was commonly used in some Asian countries for the treatment of fever, vasoconstrictive syncope, lymphangitis and other diseases, obviously due to the effect of C21 steroidal glycosides. THE AIM OF THE REVIEW: The review concentrates on the botany, ethnopharmacology, phytochemistry, pharmacology and toxicology of Cynanchum atratum. We also discuss expectations for prospective research and implementation of this herb. MATERIALS AND METHODS: Relevant information about C. atratum was gained from ancient books and records, Doctoral and master's Theses, Science Direct, Pubmed, Wiley, CNKI, WanFang DATA, Google Scholar and other domestic and foreign literature. Some electronic databases have been included. RESULTS: As a member of the Apocynaceae family, C. atratum possesses its up-and-coming biological characteristics. It is widely reported for treating of postpartum fatigue, vomiting, urine drops, nephritis, urinary tract infection, edema, bronchitis and rheumatic low back pain. By now, over 100 compounds have been identified from C. atratum, including C21 steroidal glycosides, acetophenones, alkaloids, volatile oil and other ingredients. Activities such as anti-inflammatory, anti-tumor, anti-virus, antibacterial, anti-forgetful and others have been corroborated in vivo and in vitro. In addition, many of the active ingredients, such as Cynatratoside A, Cynanversicoside A, B, D, G, p-hydroxyacetophenone, 2,4-dihydroxyacetophenone and some volatile oils have been used as quality markers. CONCLUSION: All kinds of research conducted on C.atratum, especially in field of ethnopharmacological use, phytochemicals and pharmacology have been reviewed. The herb has been used over the years in treating nephritis, urinary tract infection, bronchitis and rheumatic lumbocrural pain. Many studies have been carried out to identify compounds that play a leading role in drug activity. However, the mechanism of drug therapy remains unclear. The evidence used to prove the quality standard of medicinal materials is obviously inadequate. Besides, safety evaluation is necessary for clinical medication. Similarly, the separation of steroidal saponins and the development of new drugs will also need further discussion.

19.
Environ Res ; 204(Pt C): 112343, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34748778

RESUMO

Antibiotics pollution in lakes has been widely reported worldwide, however rare studies were concerned about antibiotics distribution in lake water - groundwater - sediment system. Here, a total of 22 antibiotics and 4 sulfonamides metabolites were detected in lake water, sediments, and different depth of groundwater surrounding Chenhu Lake during the wet and dry seasons. N4-acetylsulfonamides (Ac-SAs), fluoroquinolones (FQs), and tetracyclines (TCs) were the main groups of antibiotics in the study area. In the whole lake environment, there were more types of antibiotics in the aquatic environments than in the sediments, and the antibiotics distribution was closely related to geographical location. Specifically, the average concentration of antibiotics in groundwater decreased with an increase in sampling site distance from the lake. All antibiotics, except oxytetracycline (OTC), showed a significant decline during the dry season that could be due to the implementation of lake conservation policies, which significantly helped reducing lake pollution. There were obvious differences in the distribution of antibiotics in distinct sedimentary environments. In the surface sediments, the antibiotics content in the reclamation and the perennially flooded areas was higher than in the lakeshore area. The hydraulic interactions in the perennial flooded area facilitated the deep migration of antibiotics into lake sediments. Correlation analysis revealed a good relevance between the distribution of antibiotics in lake water and groundwater. Redundancy analysis shows that dissolved oxygen and temperature were the main factors affecting the distribution of antibiotics.

20.
Exp Ther Med ; 23(1): 85, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34938367

RESUMO

Mitochondrial malfunction leads to the remodeling of myocardial energy metabolism during myocardial ischemia (MI). However, the alterations to the mitochondrial proteome profile during this period has not yet been clarified. An acute MI model was established by high position ligation of the left anterior descending artery in 8-week-old C57BL/6N mice. After 15 min of ligation, the animals were euthanized, and their hearts were collected. The myocardial ultrastructure was observed using transmission electron microscopy (TEM). The cardiac mitochondrial proteome profile was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and bioinformatics analyses. TEM showed that the outer membrane of the mitochondria was dissolved, and the inner membrane (cristae) was corrupted and broken down extensively in the MI group. The mitochondrial membrane potential was decreased. More than 1,700 mitochondrial proteins were identified by LC-MS/MS analysis, and 119 were differentially expressed. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis showed that endopeptidase activity regulation, the mitochondrial inner membrane, oxidative phosphorylation, the hypoxia-inducible factor-1 signaling pathway, the pentose phosphate pathway and the peroxisome proliferator-activated receptor signaling pathway were involved in the pathophysiological process in the early stage of acute MI. Extensive and substantial changes in the mitochondrial proteins as well as mitochondrial microstructural damage occur in the early stages of acute MI. In the present study, the series of proteins crucially involved in the pathways of mitochondrial dysfunction and metabolism were identified. Further studies are needed to clarify the roles of these proteins in myocardial metabolism remodeling during acute MI injury.

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