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1.
PLoS One ; 18(1): e0280815, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36689428

RESUMO

Mechanical products are becoming more diversified with the continuous development of precision processing and materials technologies. The friction force generated by the O-ring seal in a hydraulic cylinder was once considered redundant. However, its utilization has recently been proposed. The hardness of the O-ring and the inner diameter of its groove directly affect the normal pressure between the O-ring and the inner wall of the cylinder, thereby affecting the friction behavior. In order to explore this friction behavior, a strain-based friction force measurement system is developed in this study, and the steady-state and dynamic friction values under different working conditions are studied and discussed in depth. This research on the friction behavior in the cylinder provides a theoretical basis for more convenient design and utilization of the friction force generated between the O-ring and the inner wall of the cylinder.


Assuntos
Fricção , Dureza , Pesquisa Empírica
2.
Chem Commun (Camb) ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36629501

RESUMO

[(CH3)3PCH2CH2CH3]2(n-C4H9NH3)Bi2Br9 (1) was obtained by a mixed organic cation strategy. The introduction of phosphonium cations increases the potential energy barrier for the motion of cations, which raises the ferroelastic phase transition temperature of 1 above room temperature, unlike the low-temperature ferroelasticity of [n-C4H9NH3]2[BiBr5]. This provides a new idea for exploring and designing molecular ferroelastic materials with excellent performance.

3.
Chemistry ; 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36598368

RESUMO

The remarkable material stability and structural diversity of two-dimensional (2D) organic-inorganic hybrid perovskites (OIHPs) constitute a vast available library of versatile materials. In particular, ferroelastic property, for which the spontaneous strain can be transformed by applying mechanical stress, is very promising for extensive nanotechnological applications. However, integrating ferroelastic property into 2D OIHPs is still in its infancy. Herein, we designed two new 2D OIHPs (C3H5CH2NH3)2[MCl4] (M = Mn for 1 and Cd for 2), which undergo reversible ferroelastic phase transitions with an Aizu expression 4/mmmFmmm. The templating influence of the more distorted inorganic framework on the disordering of organic cations and the stronger hydrogen bonds has a key role in the striking improvement of Curie temperature from 246 K in 1 to 273 K in 2. Meanwhile, the minimized alteration of structural motif ensures the well maintaining of the ferroelastic performance in the forms of crystals and thin films, as demonstrated by the identifiable evolution of domain structures. This work will provide a fertile new ground for enlarging the limited number of 2D ferroelastic OIHPs with better practical utility.

4.
ESC Heart Fail ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631006

RESUMO

AIMS: Recovery of blood flow is a therapeutic approach for myocardial infarction but paradoxically induces injury to the myocardium. Exosomes (exos) are pivotal mediators for intercellular communication that can be released by different cells and are involved in cardiovascular diseases. This study aimed to explore the possible effects and mechanisms of miR-155-5p loaded by serum-derived exos in myocardial infarction reperfusion injury (MIRI). METHODS AND RESULTS: Exos were isolated from mouse serum after induction of ischaemia reperfusion (I/R) and injected into I/R-treated mice to assess cardiac function, infarction size, and cardiomyocyte apoptosis. Primary cardiomyocytes were transfected with miR-155-5p inhibitor before treatment with oxygen-glucose deprivation and re-oxygenation (OGD/R) and exos derived from the serum of I/R-treated mice (I/R-Exos), in which Bcl-2, Bax, and cleaved-caspase-3 levels were detected. The interactions among miR-155-5p, NEDD4, and CypD were evaluated. miR-155-5p level was evidently increased in I/R-Exos than in exos from the serum of sham-operated mice (P < 0.05). In comparison with the I/R group, the I/R-Exos + I/R group had increased infarct size, elevated miR-155-5p expression, and boosted apoptotic rate in mouse myocardium (P < 0.05). In mice treated with I/R-Exos and I/R, miR-155-5p inhibition reduced cardiac infarct size and apoptosis (P < 0.05). NEDD4 was a target gene of miR-155-5p and promoted CypD ubiquitination. Cardiomyocyte apoptosis was markedly increased in the miR-155-5p inhibitor + shNEDD4 + OGD/R group versus the miR-155-5p inhibitor + OGD/R group (P < 0.05), but decreased in the miR-155-5p inhibitor + shNEDD4 + shCypD + OGD/R group than in the miR-155-5p inhibitor + shNEDD4 + OGD/R group (P < 0.05). CONCLUSIONS: miR-155-5p in I/R-Exos may facilitate MIRI by inhibiting CypD ubiquitination via targeting NEDD4.

5.
Cells ; 12(1)2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36611986

RESUMO

Feeding is crucial for the growth and survival of animals, including humans, but relatively little is known about how it is regulated. Here, we show that larval feeding in Ostrinia furnacalis is regulated by neuropeptide F (NPF, the homologous peptide of mammalian NPY) via the insulin signalling pathway in the midgut. Furthermore, the genes pi3k and mtor in the insulin pathway positively regulate α-amylase and lipase of the midgut by recruiting the transcription factors c-Myc and PPARγ for binding to the promotors of these two enzymes. Importantly, we find that the feeding behaviour and the digestive system of midgut in O. furnacalis larvae are closely related and interactive in that knocking down α-amylase or lipase induces a reduction in larval feeding, while food-deprived larvae lead to fewer expressions of α-amylase and lipase. Importantly, it is the gut NPF that regulates the α-amylase and lipase, while variations of α-amylase and lipase may feed back to the brain NPF. This current study reveals a molecular feedback mechanism between feeding behaviour and the digestive system that is regulated by the conserved NPF via insulin signalling systems in the midgut of O. furnacalis larvae.


Assuntos
Insulinas , Mariposas , Animais , Humanos , Larva/genética , Lipase , Digestão , alfa-Amilases/genética , Mamíferos
6.
Inorg Chem ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36691390

RESUMO

Two cationic zinc(II) complexes with carbazole-type counter-anions, namely, [Zn(tpy)2]2+[CAZ-p-BF3-]2 (Zn-p) and [Zn(tpy)2]2+[CAZ-o-BF3-]2 (Zn-o), have been designed and synthesized, where tpy is 2,2':6',2″-terpyridine, CAZ-p-BF3- is 4-((9H-carbazol-9-yl)phenyl)trifluoroborate, and CAZ-o-BF3- is (2-(9H-carbazol-9-yl)phenyl)trifluoroborate. The complex cation [Zn(tpy)2]2+ (as the acceptor) and the carbazole-type counter-anion CAZ-p-BF3- or CAZ-o-BF3- (as the donor) form an intracomplex donor/acceptor pair. Single-crystal structures reveal that compared to Zn-p, Zn-o exhibits a stronger π-π stacking interaction between the carbazole group (as the donor unit) of the counter-anion and the tpy ligand (as the acceptor unit) of [Zn(tpy)2]2+ because of the different anchoring position of the BF3- anion in the counter-anion. In a doped film, Zn-p and Zn-o afford an isolated exciplex formed between the carbazole group and the tpy ligand within the single complex, which gives green-yellow emission with a thermally activated delayed fluorescence (TADF) feature. In crystalline states, Zn-p and Zn-o afford exciplexes with blue emission centered at 468 nm and green-blue emission centered at 508 nm, respectively. The Zn-p crystalline sample shows a relatively large singlet-triplet energy gap (ΔEST) (0.33 eV) and no TADF, whereas the Zn-o crystalline sample exhibits a small ΔEST (0.06 eV) and distinct TADF, with a reverse intersystem crossing rate at 3.3 × 105 s-1. Zn-p and Zn-o both exhibit intriguing mechanochromic luminescence, with largely red-shifted (by over 70 nm) emission and modulated TADF properties upon mechanically grinding the crystalline samples. The work demonstrates that donor/acceptor pairs affording exciplexes can be formed within cationic metal complexes using counter-anions with donor nature, which opens a new avenue toward photo-active metal complexes with rich photophysical properties.

7.
Clin Appl Thromb Hemost ; 29: 10760296231151696, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36683414

RESUMO

OBJECTIVE: To investigate the risk factors of pulmonary embolism in patients with lung cancer and develop and validate a novel nomogram scoring system-based prediction model. METHOD: We retrospectively analyzed the clinical data and laboratory characteristics of 900 patients with lung cancer who were treated, including patients with lung cancer without pulmonary embolism (LC) and patients with lung cancer with pulmonary embolism (LC + PE). The patients were randomly divided into derivation and internal validation groups in a 7:3 ratio. Using logistic regression analysis, a diagnostic model of the nomogram scoring system was developed by incorporating selected variables in the derivation group and validated in the internal and external validation groups (n = 108). RESULT: Seven variables (adenocarcinoma, stage III-IV LC, indwelling central venous catheter, chemotherapy, and the levels of serum albumin, hemoglobin, and D-dimer) were identified as valuable parameters for developing the novel nomogram diagnostic model for differentiating patients with LC and LC + PE. The scoring system demonstrated good diagnostic performance in the derivation (area under the curve [AUC]; 95% confidence interval [CI], 0.918; 0.893, 0.943; sensitivity, 88.5%; specificity, 80.5%), internal validation (AUC; 95% CI, 0.921; 0.884, 0.958; sensitivity, 90.5%; specificity, 80.4%), and external validation (AUC; 95% CI, 0.929; 0.875, 0.983; sensitivity; 85.0%; specificity; 87.5%) groups. CONCLUSION: In this study, we constructed and validated a nomogram scoring system based on 7 clinical parameters. The scoring system exhibits good accuracy and discrimination between patients with LC and LC + PE and can effectively predict the risk of PE in patients with LC.


Assuntos
Neoplasias Pulmonares , Embolia Pulmonar , Humanos , Estudos Retrospectivos , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/patologia , Embolia Pulmonar/diagnóstico , Embolia Pulmonar/etiologia , Nomogramas , Fatores de Risco
8.
Water Res ; 230: 119493, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36634530

RESUMO

The anaerobic/aerobic/anoxic (A/O/A) process is a promising biotechnology to intensify denitrification in low carbon/nitrogen (C/N) wastewater treatment, but the neglected typical rate-limiting step-nitrification-would hinder its wider application. Heterotrophic nitrification driven by intracellular carbon (PHAs) could enhance nitrification and achieve endogenous simultaneous nitrification and denitrification (ESND) in the A/O/A process, but its feasibility remains unexamined. Here we established four A/O/A-SBRs at different C/N ratios (3, 7.5, 12, and 16.5) to address the above-mentioned knowledge gaps. The results showed that organic supplementation promoted both nitrification and denitrification (performance and relevant enzymatic activities) until organic overdose (C/N = 16.5) exacerbated niche competitions from other non-functional heterotrophs. qPCR and batch tests indicated that high C/N ratios inhibited autotrophic nitrifiers, and heterotrophic nitrifiers (HNB) dominated in the enhanced nitrification. Given the high HNB contribution (43.7%) and low COD variation (< 10 mg L-1) in the SND (76.4%) of CN12, we proposed a potential SND pathway based on heterotrophic nitrification and denitrification driven by PHAs and verified it with batch tests. Microbial and functional analyses suggested that CN12 favored the intracellular carbon transformation and harbored the minimum autotrophic nitrifiers, supporting the dominance of ESND in the enhanced SND. Our findings expand the understanding of the relationships between intracellular carbon transformation and SND and provide a novel nitrogen removal pathway for the practical application of the A/O/A process.


Assuntos
Desnitrificação , Nitrificação , Anaerobiose , Reatores Biológicos , Nitrogênio/metabolismo , Carbono/metabolismo , Suplementos Nutricionais
9.
Bioresour Technol ; 371: 128601, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36632852

RESUMO

The aim of this study was to remove the refractory organics from high-temperature anaerobic digestate food waste effluent by the coupling system of hydrolysis-acidification and denitrification. Iron-based materials (magnetite, zero-valent iron, and iron-carbon) were used to enhance the performance of thermophilic hydrolysis-acidification. Compared with the control group, magnetite had the best strengthening effect, increasing volatile fatty acids concentration and fluorescence intensity of easily biodegradable organics in the effluent by 47.6 % and 108.4 %, respectively. The coupling system of magnetite-enhanced thermophilic hydrolysis-acidification and denitrification achieved a nitrate removal efficiency of 91.2 % (influent NO3--N was 150 mg L-1), and reduced the fluorescence intensity of refractory organics by 33.8 %, compared with influent. Microbiological analysis indicated that magnetite increased the relative abundance of thermophilic hydrolytic acidifying bacteria, and coupling system enriched some genera simultaneously removing nitrate and refractory organics. This study provided fresh information on refractory organics and nitrogen removal of thermophilic wastewater biologically.

10.
BMC Musculoskelet Disord ; 24(1): 50, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36670364

RESUMO

BACKGROUND AND OBJECTIVES: Frozen shoulder (FS) is characterized by pain and significant loss of active and passive shoulder motion. Strengthening exercises are among the standard exercises used for FS. Neuromuscular exercise (NME) effectively improved pain and the range of motion in shoulder. However, no prior research has looked into the effects of NME compared to strengthening exercises in FS rehabilitation. The aim of the present study was to evaluate the effects of NME compared to strengthening exercises on pain and active range of motion (AROM) in individuals with idiopathic frozen shoulder. METHODS: Forty individuals with idiopathic frozen shoulder were randomly assigned to either the experimental group (NME with regular physical therapy, n = 20) or the control group (strengthening exercises with regular physical therapy, n = 20). In both groups, the interventions were performed once a day, 5 days a week for 8 weeks. Pain scores on the visual analogue scale (VAS) and AROM of the shoulder were assessed at baseline and after the 8-week treatment. The primary analysis was the group × time interaction. RESULTS: Two-by-two mixed analysis of variance (ANOVA) revealed a significant group × time interaction for VAS (F = 29.67; p < 0.01); AROM in flexion (F = 12.05; p < 0.01), internal rotation (F = 6.62; p < 0.05) and external rotation (F = 16.93; p < 0.01) in favor of the experimental group. The two-by-two mixed ANOVA revealed a significant main effect of time for VAS (F = 1648.47; p < 0.01); AROM in flexion (F = 591.70; p < 0.01), extension (F = 114.57; p < 0.01), abduction (F = 1602.04; p < 0.01), internal rotation (F = 664.14; p < 0.01) and external rotation (F = 1096.92; p < 0.01). No other significant differences were found. CONCLUSIONS: NME is superior to strengthening exercises in terms of pain and AROM of shoulder flexion, internal rotation and external rotation in individuals with idiopathic FS. NME could be used to treat individuals with FS. TRIAL REGISTRATION: Trial registration number: ChiCTR2100054453. Registration date: 17/12/2021.


Assuntos
Bursite , Terapia por Exercício , Humanos , Ombro , Dor , Amplitude de Movimento Articular , Bursite/terapia , Dor de Ombro/diagnóstico , Dor de Ombro/terapia , Resultado do Tratamento
11.
Inorg Chem ; 62(3): 1279-1285, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36609157

RESUMO

Ferroelastic materials have received special attention because of their great promise for mechanical switches, piezoelectric sensors, and data storage applications. Here, we report a novel ferroelastic semiconducting hybrid organic-inorganic perovskite (C3H7N2S)PbBr3 (1) [(C3H7N2S)+ is 2-amino-2-thiazolinium] with a ferroelastic phase transition at 395 K and an optical band gap of 3.43 eV. 1 has a one-dimensional BaNiO3-type structure and undergoes a high-temperature ferroelastic phase transition with an Aizu notation of mmmF2/m. Meanwhile, 1 exhibits dielectric switch near the phase transition temperature. By introducing the thioether group, the motion of the molecules or ions of 1 is hindered after the sorption of Pd(II) metal ions, which leads to the disappearance of the high-temperature ferroelastic phase transition and dielectric switch. This is the first reported ferroelastic semiconductor material with Pd(II) adsorption property, by studying the influence of Pd(II) adsorption on high-temperature ferroelastic phase transition, it may be enlightening to further uncover the mechanism of phase transition or the origin of ferroelasticity, which represents an important step toward multifunctional applications of lead-hybrid perovskite-based ferroelastic materials.


Assuntos
Temperatura , Adsorção , Transição de Fase , Temperatura de Transição
12.
Anal Chem ; 95(2): 1132-1139, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36533834

RESUMO

Extracellular vesicles (EVs) have emerged as a potential biomarker in liquid biopsy. However, cancer heterogeneity poses significant challenge to precise molecular diagnosis based on single-parameter input. Hence, strategies for analyzing multiple inputs with molecular computing were developed with the aim of improving diagnostic accuracy in liquid biopsy. In the present study, based on the surface of aptamer-encoded EVs, three toe-hold extended DNA aptamers served as specific inputs to perform AND-logic-gating to distinguish between healthy and cancerous EVs. In addition, this strategy has been successfully employed to analyze circulating EVs in clinical samples from colorectal cancer patients and healthy donors. The developed method has a promising future in the analysis of multiplex EV membrane proteins and the identification of early cancer.


Assuntos
Aptâmeros de Nucleotídeos , Neoplasias Colorretais , Vesículas Extracelulares , Humanos , Biomarcadores Tumorais/metabolismo , Vesículas Extracelulares/metabolismo , Biópsia Líquida/métodos , Aptâmeros de Nucleotídeos/metabolismo , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/metabolismo
13.
Environ Technol ; : 1-13, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36511617

RESUMO

Advanced oxidation processes (AOPs) based on ozone are gaining continuously growing popularity in wastewater treatment. This study explored the treatment of coking wastewater using a combination of ozonation (O3), ultraviolet (UV), and hydrogen peroxide (H2O2) process expressed by % chemical oxygen demand (COD) removal, % total organic carbon (TOC), % UV254, % fluorescence intensity removal and its electrical energy consumption. The obtained results demonstrated that, the combination of O3, UV, and H2O2 which is denoted by O3/UV/H2O2 in this study achieved great success in COD removal (92.08%), TOC removal (78.25%), and reduction of fluorescence intensity (99.82%). Compared with the O3 and O3/UV processes, O3/UV/H2O2 improved the COD removal by approximately 54-69% and 38-51%, respectively. In addition, the energy consumption was reduced by 53-67%. The TOC removal rate in the effluent ranged 71% and 83%, while the UV254 removal rate was up to 90%. The fluorescence spectroscopy showed that the O3/UV/H2O2 combination process reduced the fluorescence intensity by almost 97% within 10 min. Furthermore, the total polycyclic aromatic hydrocarbons (PAHs) concentration in the effluent was less than 10µg/L (removal efficiency > 80%) and the most toxic benzo(a)pyrene (BaP) was less than 0.03 µg/L (0.018µg/L). In addition, the energy consumption of the O3/UV/H2O2 process was 53-67% lower than those of O3 and O3/UV processes. Furthermore, the energy consumption was 80.26 kWh m-3 after 60 min of reaction time when the COD (69.3 mg/L) met the standard discharge. Finally, the O3/UV/H2O2 process could be an effective method for improving the mineralisation of refractory organic matter.

14.
Ecotoxicol Environ Saf ; 249: 114375, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36508836

RESUMO

Microplastics (MPs) are widespread in the environment and can be ingested through food, water, and air, posing a threat to human health. In addition, MPs can have a potential combined effect with other toxic compounds. Polystyrene (PS) has been shown to enhance the cytotoxicity of okadaic acid (OA). However, it remains unclear whether this enhancement effect is related to the size of PS particles. In this study, we investigated the mechanism of the combined effect of PS microplastics (PS-MPs) or PS nanoplastics (PS-NPs) and OA on Caco-2 cells. The results indicated that PS-NPs enhanced the cytotoxicity of OA and induced endoplasmic reticulum (ER) stress-mediated apoptosis in Caco-2 cells, compared to PS-MPs. Specifically, PS-NPs and OA cause more severe oxidative stress, lactate dehydrogenase (LDH) release, and mitochondrial membrane depolarization. Furthermore, it induced intracellular calcium overload through store-operated channels (SOCs) and activated the PERK/ATF-4/CHOP pathway to cause ER stress. ER stress promoted mitochondrial damage and finally activated the caspase family to induce apoptosis. This study provided an indirect basis for the assessment of the combined toxicity of MPs or NPs with OA.


Assuntos
Poliestirenos , Poluentes Químicos da Água , Humanos , Poliestirenos/toxicidade , Poliestirenos/metabolismo , Microplásticos/toxicidade , Plásticos , Células CACO-2 , Ácido Okadáico/toxicidade , Apoptose
15.
J Adv Res ; 43: 187-203, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36585108

RESUMO

INTRODUCTION: Atherosclerotic complications represent the leading cause of cardiovascular mortality globally. Dysfunction of endothelial cells (ECs) often initiates the pathological events in atherosclerosis. OBJECTIVES: In this study, we sought to investigate the transcriptional profile of atherosclerotic aortae, identify novel regulator in dysfunctional ECs and hence provide mechanistic insights into atherosclerotic progression. METHODS: We applied single-cell RNA sequencing (scRNA-seq) on aortic cells from Western diet-fed apolipoprotein E-deficient (ApoE-/-) mice to explore the transcriptional landscape and heterogeneity of dysfunctional ECs. In vivo validation of SOX4 upregulation in ECs were performed in atherosclerotic tissues, including mouse aortic tissues, human coronary arteries, and human renal arteries. Single-cell analysis on human aortic aneurysmal tissue was also performed. Downstream vascular abnormalities induced by EC-specific SOX4 overexpression, and upstream modulators of SOX4 were revealed by biochemical assays, immunostaining, and wire myography. Effects of shear stress on endothelial SOX4 expression was investigated by in vitro hemodynamic study. RESULTS: Among the compendium of aortic cells, mesenchymal markers in ECs were significantly enriched. Two EC subsets were subsequently distinguished, as the 'endothelial-like' and 'mesenchymal-like' subsets. Conventional assays consistently identified SOX4 as a novel atherosclerotic marker in mouse and different human arteries, additional to a cancer marker. EC-specific SOX4 overexpression promoted atherogenesis and endothelial-to-mesenchymal transition (EndoMT). Importantly, hyperlipidemia-associated cytokines and oscillatory blood flow upregulated, whereas the anti-diabetic drug metformin pharmacologically suppressed SOX4 level in ECs. CONCLUSION: Our study unravels SOX4 as a novel phenotypic regulator during endothelial dysfunction, which exacerbates atherogenesis. Our study also pinpoints hyperlipidemia-associated cytokines and oscillatory blood flow as endogenous SOX4 inducers, providing more therapeutic insights against atherosclerotic diseases.


Assuntos
Aterosclerose , Células Endoteliais , Humanos , Camundongos , Animais , Células Endoteliais/metabolismo , Aterosclerose/genética , Aterosclerose/metabolismo , Aterosclerose/patologia , Aorta/metabolismo , Citocinas/metabolismo , Análise de Célula Única , Fatores de Transcrição SOXC/genética , Fatores de Transcrição SOXC/metabolismo
16.
Rice (N Y) ; 15(1): 62, 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36463341

RESUMO

Previous studies in Arabidopsis reported that the PPR protein SOAR1 plays critical roles in plant response to salt stress. In this study, we reported that expression of the Arabidopsis SOAR1 (AtSOAR1) in rice significantly enhanced salt tolerance at seedling growth stage and promoted grain productivity under salt stress without affecting plant productivity under non-stressful conditions. The transgenic rice lines expressing AtSOAR1 exhibited increased ABA sensitivity in ABA-induced inhibition of seedling growth, and showed altered transcription and splicing of numerous genes associated with salt stress, which may explain salt tolerance of the transgenic plants. Further, we overexpressed the homologous gene of SOAR1 in rice, OsSOAR1, and showed that transgenic plants overexpressing OsSOAR1 enhanced salt tolerance at seedling growth stage. Five salt- and other abiotic stress-induced SOAR1-like PPRs were also identified. These data showed that the SOAR1-like PPR proteins are positively involved in plant response to salt stress and may be used for crop improvement in rice under salinity conditions through transgenic manipulation.

17.
BMC Biotechnol ; 22(1): 38, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36471312

RESUMO

BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform. RESULTS: A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics. CONCLUSION: The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry.


Assuntos
Doença de Newcastle , Vírus da Doença de Newcastle , Animais , Vírus da Doença de Newcastle/genética , DNA Complementar/genética , Galinhas/genética , Vacinas Combinadas
18.
BMC Urol ; 22(1): 211, 2022 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-36566200

RESUMO

BACKGROUND: To assess the characteristics, predictive risk factors, and prognostic effect of secondary bladder cancer (SBCa) following radical nephroureterectomy (RNU) in upper tract urothelial carcinoma (UTUC). METHODS: Using the Surveillance, Epidemiology, and End Results database, the authors analyzed clinicopathologic characteristics and survival data from 472 UTUC patients with SBCa after RNU, between 2004 and 2017. Cox's proportional hazard regression model was implemented to identify independent predictors associated with post-recurrence outcomes. The threshold for statistical significance was p < 0.05. RESULTS: In total, 200 Ta-3N0M0 localized UTUC patients with complete data were finally included. With a median follow-up of 71.0 months (interquartile ranges [IQR] 36.0 -103.8 months), 52.5% (n = 105) had died, with 30.5% (n = 61) dying of UTUC. The median time interval from UTUC to SBCa was 13.5 months (IQR 6.0-40.8 months). According to multivariable Cox regression analysis, patients with SBCa located at multiple sites, advanced SBCa stage, higher SBCa grade, elderly age and a shorter recurrence time, encountered worse cancer-specific survival (CSS), all p < 0.05. CONCLUSION: For primary UTUC patients with SBCa after radical surgery, advanced age, multiple SBCa sites, shorter recurrence time, higher SBCa stage, and grade proved to be significant independent prognostic factors of CSS. We ought to pay more attention to SBCa prevention as well as to earlier signs which may increase the likelihood of early detection. Having the ability to manage what may be seen as the superficial SBCa signs may enable us to improve survival but further research is required.


Assuntos
Carcinoma de Células de Transição , Neoplasias Ureterais , Neoplasias da Bexiga Urinária , Humanos , Idoso , Nefroureterectomia/métodos , Carcinoma de Células de Transição/patologia , Neoplasias da Bexiga Urinária/cirurgia , Estudos Retrospectivos , Prognóstico , Fatores de Risco , Recidiva Local de Neoplasia/cirurgia , Neoplasias Ureterais/patologia
19.
Arch Microbiol ; 205(1): 20, 2022 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-36482126

RESUMO

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a zoonotic pathogen that causes severe gastroenteritis. The 5'-nucleotidases of pathogens can dephosphorylate adenosine phosphates, boost adenosine levels and suppress the pro-inflammatory immune response. In our previous study, an extracellular nuclease, 5'-nucleotidase, was identified in the extracellular proteins of S. Typhimurium. However, the nuclease activity and the function of the 5'-nucleotidase of S. Typhimurium have not been explored. In the present study, deletion of the 5'-nucleotidase gene is dispensable for S. Typhimurium growth, even under environmental stress. Fluorescence microscopy revealed that the 5'-nucleotidase mutant induced more macrophage extracellular traps (METs) than the wild type did. Furthermore, recombinant 5'-nucleotidase protein (r5Nuc) could degrade λDNA, and the nuclease activity of r5Nuc was optimum at 37 °C and pH 6.0-7.0. The Mg2+ enhanced the nuclease activity of r5Nuc, whereas Zn2+ inhibited it. Meanwhile, deletion of the 5'-nucleotidase gene increased the bactericidal activity of METs, and r5Nuc could degrade METs and inhibit the bactericidal activity of METs. In conclusion, S. Typhimurium growth was independent of 5'-nucleotidase, but the nuclease activity of 5'-nucleotidase assisted S. Typhimurium to evade macrophage-mediated extracellular killing through degrading METs.


Assuntos
Armadilhas Extracelulares , Salmonella typhimurium , Salmonella typhimurium/genética , Macrófagos
20.
Hepatol Commun ; 6(12): 3578-3591, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36349484

RESUMO

CircRNAs have been reported to play crucial roles in tumor progression and recurrence, showing potential as biomarkers in cancer. However, the global abundance of circRNA and their involvement in hepatocellular carcinoma (HCC) development have not been fully explored. Whole transcriptome sequencing was performed on tumor and peritumor from 60 patients with HCC to quantify the expression of circRNAs, and the global circRNA abundance was calculated by circRNA index (CRI). Gene-set enrichment analysis and weighted gene co-expression network analysis were used to reveal the biological signaling pathways associated with the global circRNA abundance. The correlation between the global circRNA abundance and the infiltration level of CD8+ T cells was explored by immunohistochemical assays. Small interfering RNA was used to knock down the pre-messenger RNA spliceosome in HCC cell lines to verify the regulation of spliceosome in global circRNA abundance. We found that dysregulation of global circRNA abundance in both tumor and peritumor could lead to worse prognosis. The immunohistochemical assay further revealed that the dysregulation of global circRNA abundance in both tumor and peritumor would obstruct the CD8+ T cells from invading into the tumor, which might explain its correlation with HCC prognosis. We also demonstrated that the spliceosome genes were the main factors to regulate the global circRNA abundance in HCC, and these results were also confirmed by knockdown experiments. Conclusion: This study revealed the association between the global circRNA abundance and patients' prognosis and its underlying mechanism.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , MicroRNAs , Humanos , Carcinoma Hepatocelular/genética , RNA Circular/genética , Neoplasias Hepáticas/genética , Spliceossomos/genética , Linfócitos T CD8-Positivos/metabolismo , MicroRNAs/genética , Regulação Neoplásica da Expressão Gênica/genética , Prognóstico
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