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2.
Biochem Genet ; 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33415668

RESUMO

Inflammatory responses play significant role in infectious etiology-induced acute lung injury (ALI). Histone deacetylase 2 is found to be essential and stimulated in lipopolysaccharide (LPS)-induced ALI by regulating proinflammatory cytokines. miR-23b has been demonstrated to be downregulated in LPS-induced inflammatory injury. In this study, we aimed to explore the interaction between miR-23b and HDAC2 and their function in LPS-induced ALI. LPS treatment was induced on murine alveolar macrophage cell line MH-S. Level of miR-23b and HDAC2 were determined by real-time PCR or Western blot. Proinflammatory cytokines expression and secretion were detected by real-time PCR and ELISA assay. The levels of miR-23b and HDAC2 were manipulated by transient transfection of miRNA mimics, shRNA or overexpression vector. The interaction between miR-23b and HDAC2 were tested by Luciferase reporter assay. LPS treatment inhibited miR-23b expression, while increased HDAC2 level in MH-S cells. Proinflammatory cytokines were stimulated by LPS treatment. Knockdown of HDAC2 or overexpression of miR-23b significantly repressed the expression of proinflammatory cytokines induced by LPS. miR-23b could suppress HDAC2 expression by directly targeting to its mRNA. LPS treatment stimulated the inflammatory responses in macrophages through inhibition of miR-23b, enhanced HDAC2 expression and inducing the expression of its downstream targets TNF-α, IL-6, and IL-1ß. Overexpression of miR-23b was sufficient to suppress inflammatory responses by targeting HDAC2, making it a promising therapeutic target to ALI treatment.

3.
Cell Rep ; 34(2): 108626, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33440159

RESUMO

Macrophage-mediated inflammation is critical in the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we describe that, with high-fat, high-sucrose-diet feeding, mature TIM4pos Kupffer cells (KCs) decrease in number, while monocyte-derived Tim4neg macrophages accumulate. In concert, monocyte-derived infiltrating macrophages enter the liver and consist of a transitional subset that expresses Cx3cr1/Ccr2 and a second subset characterized by expression of Trem2, Cd63, Cd9, and Gpmnb; markers ascribed to lipid-associated macrophages (LAMs). The Cx3cr1/Ccr2-expressing macrophages, referred to as C-LAMs, localize to macrophage aggregates and hepatic crown-like structures (hCLSs) in the steatotic liver. In C-motif chemokine receptor 2 (Ccr2)-deficient mice, C-LAMs fail to appear in the liver, and this prevents hCLS formation, reduces LAM numbers, and increases liver fibrosis. Taken together, our data reveal dynamic changes in liver macrophage subsets during the pathogenesis of NASH and link these shifts to pathologic tissue remodeling.

4.
Nat Commun ; 12(1): 60, 2021 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-33397900

RESUMO

Long nanopore reads are advantageous in de novo genome assembly. However, nanopore reads usually have broad error distribution and high-error-rate subsequences. Existing error correction tools cannot correct nanopore reads efficiently and effectively. Most methods trim high-error-rate subsequences during error correction, which reduces both the length of the reads and contiguity of the final assembly. Here, we develop an error correction, and de novo assembly tool designed to overcome complex errors in nanopore reads. We propose an adaptive read selection and two-step progressive method to quickly correct nanopore reads to high accuracy. We introduce a two-stage assembler to utilize the full length of nanopore reads. Our tool achieves superior performance in both error correction and de novo assembling nanopore reads. It requires only 8122 hours to assemble a 35X coverage human genome and achieves a 2.47-fold improvement in NG50. Furthermore, our assembly of the human WERI cell line shows an NG50 of 22 Mbp. The high-quality assembly of nanopore reads can significantly reduce false positives in structure variation detection.


Assuntos
Nanoporos , Análise de Sequência de DNA , Linhagem Celular , Cromossomos Humanos/genética , Genoma Humano , Humanos , Retinoblastoma/genética , Software
5.
Ecotoxicol Environ Saf ; 210: 111853, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33422838

RESUMO

A multi-residue analysis of six diacylhydrazine insecticides in water, sediment, and aquatic products was established by liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS). The water sample was extracted with acetonitrile by low-temperature enrichment liquid-liquid extraction technology. The sediment and aquatic products were prepared using QuEChERS technique. Method validation showed perfect linearity with correlation coefficients (R) more than 0.9992 for all insecticides, and the matrix effects were nearly negligible (-1.42% to -0.27%) for water, sediment and aquatic products. The recoveries were 80.0-99.7% at three spiked levels (0.02 ng·mL-1, 0.1 ng·mL-1, 0.5 ng·mL-1; 2.0, 10, and 50 ng·g-1) and the precisions (intra-day and inter-day precision) were lower than 5.28%, with the low LODs (3.8 ~ 9.6 pg·mL-1; 0.38-0.96 ng·g-1) and LOQs (12.7 ~ 32.0 pg·mL-1; 1.27-3.20 ng·g-1) for water, sediment, and aquatic products, indicating the good accuracy and precision of the proposed method. The applicability, efficiency, and sensitivity of this method have been proved in the analysis of six diacylhydrazine insecticides in water, sediment, and crucian carp in Rice- crucian carp - integrated planting system.

6.
Int J Biol Macromol ; 171: 539-549, 2021 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-33434550

RESUMO

The present study aimed to investigate the biological functions of germinated M. oleifera seed proteins and to identify the identity of milk-clotting proteases. A total of 963 proteins were identified, and those with molecular weights between 10 and 30 kDa were most abundant. The identified proteins were mainly involved in energy-associated catalytic activity and metabolic processes, and carbohydrate and protein metabolisms. The numbers of proteins associated with the hydrolytic and catalytic activities were higher than the matured dry M. oleifera seeds reported previously. Of the identified proteins, proteases were mainly involved in the milk-clotting activity. Especially, a cysteine peptidase with a molecular mass of 17.727 kDa exhibiting hydrolase and peptidase activities was purified and identified. The identified cysteine peptidase was hydrophilic, and its secondary structure consisted of 27.60% alpha helix, 9.20% beta fold, and 63.20% irregular curl; its tertiary structure was also constructed using M. oleifera seed 2S protein as the protein template. The optimal pH and temperature of the purified protease were pH 4.0 and 60 °C, respectively. The protease had high acidic stability and good thermostability, thus could potentially be applied in the dairy industry.

7.
Arch Toxicol ; 2021 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-33458792

RESUMO

Azoxymethane (AOM) is a widely used carcinogen to study chemical-induced colorectal carcinogenesis and is an agent for studying fulminant hepatic failure. The inter-strain susceptibility to acute toxicity by AOM has been reported, but its association with host genetics or gut microbiota remains largely unexplored. Here a cohort of genetically diverse Collaborative Cross (CC) mice was used to assess the contribution of host genetics and the gut microbiome to AOM-induced acute toxicity. We observed variation in AOM-induced acute liver failure across CC strains. Quantitative trait loci (QTL) analysis revealed three chromosome regions significantly associated with AOM toxicity. Genes located within these QTL, including peroxisome proliferator-activated receptor alpha (Ppara), were enriched for enzyme activator and nucleoside-triphosphatase regulator activity. We further demonstrated that the protein level of PPARα in liver tissues from sensitive strains was remarkably lower compared to levels in resistant strains, consistent with protective role of PPAR family in liver injury. We discovered that the abundance levels of gut microbial families Anaeroplasmataceae, Ruminococcaceae, Lactobacillaceae, Akkermansiaceae and Clostridiaceae were significantly higher in the sensitive strains compared to the resistant strains. Using a random forest classifier method, we determined that the relative abundance levels of these microbial families predicted AOM toxicity with the area under the receiver-operating curve (AUC) of 0.75. Combining the three genetic loci and five microbial families increased the predictive accuracy of AOM toxicity (AUC of 0.99). Moreover, we found that Ruminococcaceae and Lactobacillaceae acted as mediators between host genetics and AOM toxicity. In conclusion, this study shows that host genetics and specific microbiome members play a critical role in AOM-induced acute toxicity, which provides a framework for analysis of the health effects from environmental toxicants.

8.
Lasers Med Sci ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33400013

RESUMO

Due to the increase of the world's population aging, how to restore youthfulness to the skin has attracted much attention. It is well known that collagen synthesis and changes in skin barrier play an important role in the process of skin aging. However, whether Q-switched 1064-nm Nd:YAG laser (1064-QSNYL) determines the involvement of miRNAs in skin collagen synthesis and skin barrier changes remains to be elucidated. Upstream miRNAs of p38 molecular pathway have been predicted by bioinformatic database and the relationship between miRNAs and p38 verified by dual-luciferase reporter gene and Western blotting. RT-qPCR analysis detected the expression of miR-24-3p and mRNA for collagen and skin barrier-related molecules, such as keratin 10 (K10), filaggrin, and Aquaporin 4 (APQ4), in mice back skin and in the keratinocyte cell line HaCaT. Western blotting and immunofluorescence (IF) have been used to detect collagen expression and to localize, as well as quantify K10, filaggrin, and APQ4, respectively. In this study, we show that p38 is the main target gene of miRNA-24-3p, and laser irradiation at 1.5 J/cm2 inhibits miR-24-3p expression. Irradiation treatment upregulates the moisture, elasticity, hydroxyproline, and superoxide dismutase content of mice skin, as well as inhibits trans-epidermal water loss. Irradiation also increases collagen, K10, filaggrin, and APQ4 in both mice skin and HaCaT cells. Interestingly, we found that miR-24-3p overexpression inhibits the effect of irradiation on collagen synthesis and skin barrier. We show for the first time that 1064-QSNYL promotes collagen synthesis and protective effects on skin barrier by downregulating miR-24-3p.

9.
Carbohydr Polym ; 251: 117062, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142614

RESUMO

In this study, Lactococcus lactis was embedded in a film of corn starch (NS) and carboxymethyl cellulose (CMC) prepared using a casting method. At a CMC:NS ratio of 5:5, the composite film had the best comprehensive properties. Scanning electron microscopy images clearly showed that L. lactis was effectively embedded. The film with 1.5 % L. lactis showed the best performance and the lowest water vapor transmission rate (5.54 × 10-11 g/m s Pa. In addition, the edible film retained a viable count of 5.64 log CFU/g of L. lactis when stored at 4 °C for 30 days. The composite film with 1.5 % L. lactis showed the highest release of nisin (3.35 mg/mL) and good antibacterial activity against Staphylococcus aureus (53.53 %) after 8 days. Therefore, this edible film is a viable alternative antimicrobial strategy for the active packaging of foods containing low moisture content.

10.
J Cosmet Dermatol ; 20(2): 481-490, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32585741

RESUMO

AIMS: This study is to evaluate the clinical efficacy and safety of a "double-layer" mode of super pulse fractional CO2 laser and a combined treatment of pinprick therapy with fractional CO2 laser for the treatment of atrophic acne scars. METHODS: A split-face and self-contrast method was applied. A total of 20 patients with atrophic acne scars randomly received the above mentioned therapy on the left or right side of the cheek for 3 times with an interval of 3 months. ECCA scores, IVA scores, patients' satisfaction, VAS scores, and adverse reactions were evaluated. RESULTS: Atrophic acne scars on both sides of the cheek were improved obviously. The ECCA scores showed a significant decrease, while IVA scores significantly increased. Compared with the super pulse fractional CO2 laser group, the decrease of ECCA scores, the increase of IVA scores, and the patients' satisfaction were significantly higher and improvement on V-shaped and U-shaped acne scars was significantly better in the combined treatment group. No patients had severe adverse reactions such as blister, infection, or hypertrophic scars. CONCLUSIONS: Super pulse fractional CO2 laser and pinprick treatment combined with fractional CO2 laser are both safe and effective therapy for the treatment of atrophic acne scars.

11.
FEBS Lett ; 595(1): 68-84, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33040326

RESUMO

Hepatocellular carcinoma (HCC) is the most common liver cancer with high mortality. Here, we found that hnRNPU is overexpressed in HCC tissues and is correlated with the poor prognosis of HCC patients. Besides, hnRNPU is of high significance in regulating the proliferation, apoptosis, self-renewal, and tumorigenic potential of HCC cells. Mechanismly, c-Myc regulates hnRNPU expression at the transcriptional level, and meanwhile, hnRNPU stabilizes the mRNA of c-MYC. We found that the hnRNPU and c-Myc regulatory loop exerts a synergistic effect on the proliferation and self-renewal of HCC, and promotes the HCC progression. Taken together, hnRNPU functions as a novel transcriptional target of c-Myc and promotes HCC progression, which may become a promising target for the treatment of c-Myc-driven HCC.

12.
Biochim Biophys Acta Gen Subj ; 1865(1): 129756, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33010351

RESUMO

BACKGROUND: Crotonase superfamily members exhibit great catalytic diversity towards various acyl-CoA substrates. A common CoA moiety binding pattern is usually observed in this family, understanding the substrate-binding mechanism would facilitate the rational engineering of crotonases for improved properties. METHODS: We applied X-ray crystallography to investigate a putative enoyl-CoA hydratase/isomerase OdaA in Pseudomonas aeruginosa. Thermal shift assay (TSA) were performed to explore the binding of OdaA with CoA thioester substrates. Furthermore, we performed molecular dynamics (MD) simulations to elucidate the dynamics of its CoA-binding site. RESULTS: We solved the crystal structures of the apo and CoA-bound OdaA. Thermal shift assay (TSA) showed that CoA thioester substrates bind to OdaA with a different degree. MD simulations demonstrated that the C-terminal alpha helix underwent a structural transition and a hinge region would associate with this conformational change. CONCLUSIONS: TSA in combination with MD simulations elucidate that the dynamics of C-terminal alpha helix in CoA-binding, and a hinge region play an important role in conformational change. GENERAL SIGNIFICANCE: Those results help to extend our knowledge about the nature of crotonases and would be informative for future mechanistic studies and industry applications.


Assuntos
Enoil-CoA Hidratase/química , Pseudomonas aeruginosa/enzimologia , Cristalografia por Raios X , Enoil-CoA Hidratase/metabolismo , Humanos , Simulação de Dinâmica Molecular , Conformação Proteica , Conformação Proteica em alfa-Hélice , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/química , Pseudomonas aeruginosa/metabolismo
13.
Age Ageing ; 50(1): 190-197, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-32556149

RESUMO

BACKGROUND: multimorbidity contributes to a large portion of the disease burden in low- and middle-income countries. However, limited research has been undertaken in China. This study has investigated the prevalence of multimorbidity and the associations of multimorbidity with activities of daily living (ADL), instrumental activities of daily living (IADL) and depression in China. METHODS: the study participants included 10,055 adults aged 45 years and older from three rounds of the China Health and Retirement Longitudinal Study 2011-2015. Random-effects logistic regression models were used to examine the association of multimorbidity with ADL limitation, IADL limitation and mental disease. RESULTS: the prevalence of multimorbidity amongst adults in China aged 45 years and older was 62.1% in 2015. The prevalence of multimorbidity was increased with older age, among women, in a higher socio-economic group and in the most deprived regions. Multimorbidity is associated with an increased likelihood of experiencing ADL limitation (adjusted odds ratio [AOR] = 5.738, 95% confidence intervals (CI) = 5.733, 5.744) and IADL limitation (AOR = 2.590, 95% CI = 2.588, 2.592) and depression (AOR = 3.352, 95% CI = 3.350, 3.354). Rural-urban disparities in functional difficulties and depression were also found amongst patients with multimorbidity. CONCLUSIONS: the burden of multimorbidity is high in China, particularly amongst the older population. Multimorbidity is associated with higher levels of functional limitations and depression. China healthcare reforms should introduce integrated care models and patient-centred healthcare delivery. The increasing need for reorientation of healthcare resources considering the distribution of multimorbidity and its adverse effect requires more attention from health policymakers in China and other developing countries.

14.
Nucleic Acids Res ; 49(D1): D998-D1003, 2021 01 08.
Artigo em Inglês | MEDLINE | ID: mdl-33084874

RESUMO

OGEE is an Online GEne Essentiality database. Gene essentiality is not a static and binary property, rather a context-dependent and evolvable property in all forms of life. In OGEE we collect not only experimentally tested essential and non-essential genes, but also associated gene properties that contributes to gene essentiality. We tagged conditionally essential genes that show variable essentiality statuses across datasets to highlight complex interplays between gene functions and environmental/experimental perturbations. OGEE v3 contains gene essentiality datasets for 91 species; almost doubled from 48 species in previous version. To accommodate recent advances on human cancer essential genes (as known as tumor dependency genes) that could serve as targets for cancer treatment and/or drug development, we expanded the collection of human essential genes from 16 cell lines in previous to 581. These human cancer cell lines were tested with high-throughput experiments such as CRISPR-Cas9 and RNAi; in total, 150 of which were tested by both techniques. We also included factors known to contribute to gene essentiality for these cell lines, such as genomic mutation, methylation and gene expression, along with extensive graphical visualizations for ease of understanding of these factors. OGEE v3 can be accessible freely at https://v3.ogee.info.

15.
Food Chem ; 344: 128632, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33229146

RESUMO

In this study, the effects of high hydrostatic pressure (HHP) and steam on biochemical composition and non-volatile taste active compounds of oysters Crassostrea hongkongensis were investigated. The moisture content in steamed oysters significantly decreased when compared to raw samples, subsequently their crude protein, crude lipid, glycogen and ash contents (% wet weight) were all increased (P < 0.05). In addition, though the moisture content in HHP oysters decreased, no significant differences were observed in proximate compositions compared to raw oysters, except crude protein. There were no significant differences in saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) profiles between raw and HHP oysters, however, C20:3n6 content in HHP oysters was significantly higher than that in raw samples (P < 0.05). The PUFA profile of steamed oysters, mostly contributed by n-3 PUFA, was significantly higher than that of both raw and HHP samples (P < 0.05). Major free amino acids (FAA) (taste activity value, TAV > 1) in oysters with three treatments were alanine, glycine, glutamic acid and histidine, and their contents were significantly higher in raw and HHP groups than that in steamed group. The 5'-inosine monophosphate (IMP) and 5'-guanosine monophosphate (GMP) in HHP and steamed oysters decreased compared to raw samples, while AMP content in steam oysters were significantly increased (P < 0.05). The equivalent umami concentration (EUC) of oysters of raw, HHP and steamed groups were 8.80, 3.66 and 1.44 g MSG/100 g, respectively, with significant differences observed among different treatments (P < 0.05). Succinic acid was the major organic acid in raw and HHP oysters, while lactic acid was the major organic acid in steamed groups. Further, Na+, K+, PO43- and Cl- were the main inorganic ions (TAV > 1), and their contents were significantly higher in raw and HHP groups than that in steamed group (P < 0.05). This study demonstrated that HHP treatment slightly influenced the changes in the biochemical composition and non-volatile taste active compounds to raw oysters, compared to steamed process.

16.
J Colloid Interface Sci ; 586: 551-562, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33246653

RESUMO

A novel in-situ N-doped carbon nanoparticles (NCNs) was prepared through direct pyrolysis of N-rich polyaniline (PANI) without using external N-containing precursor and the as-prepared materials were employed as metal-free peroxydisulfate (PDS) activator for bisphenol A (BPA) degradation. The catalyst derived from PANI carbonization at 900 °C (NCNs-9) displayed the excellent catalytic activity to activate PDS, resulting in 96.0% BPA degradation efficiency within 20 min. The catalytic activity of NCNs was closely related to their structure-composition, and higher graphitic N content and larger BET surface area were beneficial to the generation of reactive oxygen species (ROS). The quenching tests and electron paramagnetic resonance (EPR) demonstrated that BPA degradation in PDS/NCNs system was accomplished via non-radical (1O2) and radical ( ·OH, SO4·-, and O2·-) pathways, in which O2·- was the main ROS. The origin of O2·- was the conversion of dissolved oxygen and the activation of PDS. The possible degradation pathways of BPA were also proposed. This study might provide inspirations to design in-situ N-doped carbon nanoparticles as the PDS activator for efficient degradation of persistent organic compound via advanced oxidation processes (AOPs).

17.
Clin Cardiol ; 44(1): 108-115, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33179816

RESUMO

BACKGROUND: The changes in right ventricular (RV) contractility of Kawasaki disease (KD) still remain unclear. HYPOTHESIS: We aimed to determine whether RV systolic dysfunction can be detected by cardiac magnetic resonance (CMR) feature tracking and to find its association with coronary artery lesions (aneurysm, thrombosis and stenosis). METHODS: Peak systolic myocardial longitudinal, radial and circumferential strain and the strain rate (RVSL, RVSR, RVSC, RVSRL, RVSRR and RVSRC) in the global RV and three levels (basal, middle and apical) were measured in 66 patients with convalescent KD. A total of 20 controls were included. Comparisons were made with controls and among KD subgroups divided with coronary artery lesions. RESULTS: RVSC (-10.575% vs. -10.760%), RVSL (-18.150% vs. -18.712%) and RVSRC (-0.815/s vs. -0.924/s) were slightly lower in KD group without significant difference. All the strain and strain rate presented lowest in the basal level. In subgroup comparison, lower RVSL and RVSRL were observed in the giant coronary artery aneurysm (CAA) group; RVSR (15.844% vs. 16.897%), RVSRR (1.245/s vs. 1.322/s) and RVSRC (-0.715/s vs. -0.895/s) were lower in thrombosed group; RVSRL (-1.27/s vs. -1.503/s) were lower in stenosis group. All the comparison in subgroups did not reach significant difference. From the analysis of receiver operating characteristic curve, RVSRL had a better ability to identify KD with giant CAA and stenosis. For the identification of thrombosis, RVSRC had a better ability. CONCLUSIONS: Lower strain and strain rates of RV were detected in convalescent KD. More pronounced in those with persisting coronary artery lesions.

18.
Chemosphere ; 264(Pt 2): 128548, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33059291

RESUMO

The electro-assisted autohydrogenotrophic reduction of perchlorate (ClO4-) was investigated in a dual-chamber biofilm-electrode reactor (BER), in which the microbial community was inoculated from natural sediments. To avoid the effect of extreme pH and direct electron transfer on perchlorate reduction, a novel cathode configuration was designed. The pH of the cathode compartment was successfully controlled in the range of 7.2-8.4 during whole experiment. The effective biological autohydrogenotrophic reduction of perchlorate was achieved using hydrogen generated in-situ on the electrode surface, and the removal rate of 10 mg L-1 perchlorate reached 98.16% at HRT of 48 h. The highest perchlorate removal flux reached to 1498.420 mg m-2·d-1 with a 0.410 kW·h g-perchlorate-1 energy consumption. The microbial community evolution in the BER was determined by high-throughput sequencing and the results indicated that the Firmicutes and Bacteroidetes were dominant at phylum level when perchlorate concentration was 10 mg L-1 or lower. And the Proteobacteria became ascendant at the perchlorate concentration of 20 mg L-1. The functional populations for perchlorate reduction were successfully enriched including Nitrosomonas (30%), Thermomonas (9%), Comamonas (8%) and Hydrogenophaga (3%). Meanwhile, the proportion of functional population in biofilm linked to perchlorate concentration. With the increase of influent perchlorate concentration, the perchlorate-reducing bacteria (PRB) were enriched successfully and became ascendant.


Assuntos
Microbiota , Percloratos , Bactérias/genética , Biofilmes , Reatores Biológicos , Eletrodos , Nitratos , Oxirredução
19.
Mol Med Rep ; 23(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33179106

RESUMO

Triple­negative breast cancer (TNBC) is characterized by strong invasiveness, frequent local recurrence and distant metastasis, with poor prognosis. According to tumor angiogenesis theory, tumor cells can obtain blood supply not only by fusing with host blood vessels, but also by constructing a new vascular system through angiogenesis, so as to continuously obtain nutrients and oxygen supply; this is called vasculogenic mimicry (VM). In our previous study, differential expression profiles of miRNAs were examined with gene chip in TNBC and corresponding paracancer tissues, which demonstrated significant up­regulation of microRNA (miR)­93. Bioinformatics found that the target genes of miR­93 were associated with cell proliferation, invasion and migration. The present study investigated the association between miR­93, epithelial­to­mesenchymal transition (EMT) and VM formation in TNBC cell lines. The results indicated that miR­93 depletion suppressed MDA­MB­231 cell viability, invasion and migration (P<0.001). In addition, knockdown of miR­93 significantly upregulated the expression levels of EMT­associated genes such as E­cadherin and occludin, but downregulated the expression levels of vimentin and N­cadherin in MDA­MB­231 cells. VM formation assay showed a significant decrease in microtubule­forming ability of cells following miR­93 knockdown, which was associated with the occurrence of EMT, suggesting that miR­93 may promote the formation of VM via EMT and may be a therapeutic target for the treatment of TNBC.

20.
Talanta ; 221: 121498, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076101

RESUMO

Due to the ultra-weak and instantaneous chemiluminescence (CL) of luminol-H2O2 system, developing new catalysts to dramatically enhance and produce persistent CL emission is crucial. In this study, the Cu-metal-organic frameworks (Cu-MOFs) with flower morphology converted from Cu-based metal-organic gels (Cu-MOGs) could catalyze luminol-H2O2 system, exhibiting persistent CL. The possible mechanism of this persistent emission of luminol was attributed to the gradual generation of OH˙, O2˙- and 1O2 in Cu-MOFs-catalyzed luminol-H2O2 system. In addition, OH˙ and O2˙- were continuously recombined into singlet oxygen on the surface of Cu-MOFs which further prolonged the duration time of the CL leading to the persistent emission, due to the longer lifetime of 1O2 compared to OH˙ and O2˙-. Furthermore, based on the high-intensity emission of Cu-MOFs-catalyzed luminol-H2O2 system, the strategy for sensitive response to quercetin was established with good linearity within the range of 0.05-1.2 µM and a detection limit of 49.7 nM. This study provides a new idea for developing persistent CL catalysts.

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